AlfalfaGEDB | Msa0500300

M. sativa cultivar zhongmu-4 / Msa0500300

KEGG: K14491
GO: GO:0000156 GO:0000160 GO:0001101 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009755 GO:0009888 GO:0009889 GO:0009966 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010073 GO:0010074 GO:0010075 GO:0010082 GO:0010380 GO:0010468 GO:0010492 GO:0010556 GO:0010646 GO:0019219 GO:0019222 GO:0019827 GO:0022622 GO:0023051 GO:0023052 GO:0031323 GO:0031326 GO:0031537 GO:0032501 GO:0032502 GO:0032870 GO:0035556 GO:0040008 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048364 GO:0048367 GO:0048507 GO:0048509 GO:0048580 GO:0048583 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051193 GO:0051239 GO:0051252 GO:0051716 GO:0060089 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071368 GO:0071495 GO:0080022 GO:0080036 GO:0080050 GO:0080090 GO:0080113 GO:0090056 GO:0098727 GO:0099402 GO:0140110 GO:1901401 GO:1901463 GO:1901700 GO:1903506 GO:1905392 GO:2000026 GO:2000112 GO:2000241 GO:2000280 GO:2001141
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500300	KEH35774.1	92.935	184	13	0	35	218	1	184	4.17e-114	333

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500300	sp\|O49397\|ARR10_ARATH	53.604	222	57	1	40	215	11	232	7.76e-73	233

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500300	A0A072V176	92.935	184	13	0	35	218	1	184	1.99e-114	333

TFs/TRs:

Gene ID	Type	Classification
Msa0500300	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500300	MtrunA17_Chr3g0134481	92.935	184	13	0	35	218	1	184	3.84e-118	333
Msa0500300	MtrunA17_Chr3g0134461	73.846	195	40	2	35	218	1	195	5.86e-89	260
Msa0500300	MtrunA17_Chr3g0137101	84.211	133	21	0	35	167	1	133	2.27e-73	234
Msa0500300	MtrunA17_Chr3g0137101	76.471	51	12	0	165	215	197	247	7.03e-20	87.4
Msa0500300	MtrunA17_Chr3g0123131	45.339	236	67	2	42	215	13	248	2.00e-60	198
Msa0500300	MtrunA17_Chr8g0376881	45.763	236	68	2	40	215	23	258	7.60e-60	196
Msa0500300	MtrunA17_Chr2g0294911	43.404	235	75	2	39	215	26	260	1.78e-59	196
Msa0500300	MtrunA17_Chr2g0304841	66.187	139	44	1	29	167	8	143	5.52e-58	194
Msa0500300	MtrunA17_Chr2g0304841	66.667	51	17	0	165	215	212	262	5.70e-16	76.3
Msa0500300	MtrunA17_Chr1g0149841	69.767	129	39	0	39	167	2	130	2.71e-57	188
Msa0500300	MtrunA17_Chr1g0149841	74.510	51	13	0	165	215	190	240	8.27e-18	81.3
Msa0500300	MtrunA17_Chr1g0149851	66.923	130	43	0	39	168	2	131	8.37e-55	183
Msa0500300	MtrunA17_Chr7g0275891	67.692	130	41	1	39	167	2	131	1.35e-54	177
Msa0500300	MtrunA17_Chr7g0275891	51.562	64	18	1	165	215	190	253	8.16e-13	66.2
Msa0500300	MtrunA17_Chr4g0067981	58.647	133	55	0	37	169	26	158	1.48e-52	179
Msa0500300	MtrunA17_Chr4g0067981	66.667	51	17	0	165	215	214	264	2.16e-16	77.4
Msa0500300	MtrunA17_Chr7g0275401	57.051	156	63	1	39	194	2	153	7.27e-51	166
Msa0500300	MtrunA17_Chr7g0275401	62.745	51	19	0	165	215	191	241	3.91e-15	72.4
Msa0500300	MtrunA17_Chr4g0074411	59.854	137	53	1	31	165	5	141	8.96e-50	171
Msa0500300	MtrunA17_Chr4g0074411	64.286	56	19	1	160	215	196	250	1.28e-15	75.1
Msa0500300	MtrunA17_Chr4g0074401	64.516	124	44	0	42	165	17	140	1.62e-48	167
Msa0500300	MtrunA17_Chr4g0074401	64.706	51	18	0	165	215	202	252	4.13e-15	73.6
Msa0500300	MtrunA17_Chr1g0161431	57.042	142	56	1	38	174	7	148	2.89e-46	161
Msa0500300	MtrunA17_Chr1g0161431	64.706	51	18	0	165	215	199	249	3.90e-16	76.6
Msa0500300	MtrunA17_Chr4g0009851	36.916	214	99	2	37	215	6	218	8.00e-42	144
Msa0500300	MtrunA17_Chr4g0009901	34.928	209	100	2	42	215	10	217	1.69e-36	129
Msa0500300	MtrunA17_Chr4g0074421	68.000	75	24	0	91	165	1	75	1.20e-30	111
Msa0500300	MtrunA17_Chr4g0074421	62.745	51	19	0	165	215	132	182	1.40e-15	72.4
Msa0500300	MtrunA17_Chr4g0009981	35.976	164	70	1	87	215	2	165	6.27e-30	111
Msa0500300	MtrunA17_Chr6g0471041	32.701	211	108	2	41	217	13	223	3.35e-29	112
Msa0500300	MtrunA17_Chr1g0149831	70.149	67	20	0	101	167	1	67	1.28e-26	102
Msa0500300	MtrunA17_Chr1g0149831	64.706	51	15	1	165	215	127	174	1.24e-13	67.4
Msa0500300	MtrunA17_Chr8g0391911	28.692	237	107	5	42	217	9	244	1.08e-24	97.8
Msa0500300	MtrunA17_Chr3g0127941	37.302	126	74	2	46	167	56	180	8.67e-21	90.1
Msa0500300	MtrunA17_Chr4g0030271	39.669	121	70	1	46	163	89	209	8.93e-21	90.1
Msa0500300	MtrunA17_Chr1g0181811	38.017	121	72	2	46	163	85	205	1.15e-20	89.7
Msa0500300	MtrunA17_Chr8g0383961	35.294	136	83	1	41	176	13	143	3.97e-20	85.9
Msa0500300	MtrunA17_Chr8g0345901	38.655	119	70	1	48	163	38	156	4.43e-19	85.1
Msa0500300	MtrunA17_Chr7g0276361	37.226	137	75	3	35	163	1	134	1.36e-18	83.6
Msa0500300	MtrunA17_Chr6g0456631	32.540	126	82	3	40	163	7	131	1.18e-17	77.0
Msa0500300	MtrunA17_Chr4g0061021	33.065	124	80	1	46	166	33	156	6.04e-17	79.0
Msa0500300	MtrunA17_Chr4g0011071	28.676	136	62	1	92	192	39	174	6.17e-17	75.5
Msa0500300	MtrunA17_Chr3g0091641	31.884	138	89	2	32	166	12	147	1.43e-16	77.8
Msa0500300	MtrunA17_Chr8g0350761	30.882	136	85	2	45	171	20	155	3.32e-15	73.6
Msa0500300	MtrunA17_Chr5g0400241	38.211	123	70	3	42	162	14	132	1.26e-14	72.0
Msa0500300	MtrunA17_Chr5g0400241	50.980	51	25	0	165	215	310	360	1.53e-11	63.2
Msa0500300	MtrunA17_Chr6g0473731	28.148	135	56	1	122	215	10	144	8.01e-14	69.7
Msa0500300	MtrunA17_Chr4g0031961	52.941	51	24	0	165	215	130	180	1.18e-12	65.9
Msa0500300	MtrunA17_Chr4g0054981	52.941	51	24	0	165	215	309	359	1.09e-11	63.5
Msa0500300	MtrunA17_Chr4g0054981	33.607	122	77	2	42	162	14	132	1.54e-11	63.2
Msa0500300	MtrunA17_Chr4g0040481	49.020	51	26	0	165	215	157	207	1.50e-11	62.8
Msa0500300	MtrunA17_Chr7g0255711	49.020	51	26	0	165	215	90	140	2.06e-11	62.0
Msa0500300	MtrunA17_Chr2g0324891	49.020	51	26	0	165	215	160	210	2.69e-11	62.4
Msa0500300	MtrunA17_Chr8g0352871	28.986	138	78	3	48	165	11	148	4.28e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0500300	AT4G31920.1	53.604	222	57	1	40	215	11	232	7.89e-74	233
Msa0500300	AT2G25180.1	69.841	126	38	0	40	165	11	136	7.82e-58	192
Msa0500300	AT2G25180.1	66.667	51	17	0	165	215	194	244	3.66e-16	77.0
Msa0500300	AT5G58080.1	46.414	237	69	3	36	215	8	243	1.44e-54	184
Msa0500300	AT1G67710.1	43.644	236	70	5	43	215	8	243	3.78e-50	171
Msa0500300	AT3G16857.1	54.861	144	63	1	42	185	33	174	5.69e-50	172
Msa0500300	AT3G16857.1	68.627	51	16	0	165	215	236	286	2.14e-16	77.8
Msa0500300	AT3G16857.2	54.861	144	63	1	42	185	33	174	7.14e-50	172
Msa0500300	AT3G16857.2	68.627	51	16	0	165	215	236	286	2.29e-16	77.8
Msa0500300	AT2G01760.1	57.031	128	54	1	39	165	4	131	3.30e-43	149
Msa0500300	AT2G01760.1	62.745	51	19	0	165	215	199	249	3.60e-16	76.6
Msa0500300	AT2G01760.2	46.795	156	54	2	39	165	4	159	3.08e-37	134
Msa0500300	AT2G01760.2	61.538	52	20	0	164	215	226	277	4.14e-16	76.6
Msa0500300	AT3G16857.3	55.238	105	45	1	81	185	2	104	3.32e-34	129
Msa0500300	AT3G16857.3	68.627	51	16	0	165	215	166	216	2.26e-16	77.8
Msa0500300	AT5G49240.1	31.535	241	104	3	39	218	35	275	2.75e-29	110
Msa0500300	AT1G67710.2	41.011	178	42	5	101	215	1	178	5.26e-28	110
Msa0500300	AT3G62670.1	32.218	239	103	4	34	215	29	265	6.09e-27	107
Msa0500300	AT1G49190.1	30.000	240	103	4	41	215	29	268	1.40e-26	105
Msa0500300	AT1G49190.2	37.600	125	78	0	41	165	29	153	1.61e-22	95.5
Msa0500300	AT5G24470.1	34.899	149	88	3	44	184	48	195	9.96e-22	93.2
Msa0500300	AT2G01760.3	62.121	66	24	1	101	165	1	66	1.50e-20	88.2
Msa0500300	AT2G01760.3	62.745	51	19	0	165	215	134	184	2.71e-16	76.6
Msa0500300	AT2G46790.1	37.705	122	71	2	46	163	37	157	4.46e-20	88.2
Msa0500300	AT2G46790.3	37.705	122	71	2	46	163	37	157	4.69e-20	88.2
Msa0500300	AT5G60100.7	38.017	121	72	1	46	163	64	184	1.94e-19	85.9
Msa0500300	AT5G60100.6	38.017	121	72	1	46	163	64	184	1.94e-19	85.9
Msa0500300	AT5G60100.4	38.017	121	72	1	46	163	64	184	1.94e-19	85.9
Msa0500300	AT5G02810.1	38.843	121	71	1	46	163	78	198	2.51e-19	86.3
Msa0500300	AT5G60100.2	38.017	121	72	1	46	163	64	184	2.71e-19	85.9
Msa0500300	AT5G60100.5	38.017	121	72	1	46	163	64	184	3.33e-19	85.9
Msa0500300	AT5G60100.3	38.017	121	72	1	46	163	64	184	3.33e-19	85.9
Msa0500300	AT5G60100.1	38.017	121	72	1	46	163	64	184	3.33e-19	85.9
Msa0500300	AT5G61380.1	34.677	124	78	1	46	166	19	142	9.16e-17	79.0
Msa0500300	AT1G67710.3	34.810	158	40	5	121	215	12	169	1.04e-15	75.5
Msa0500300	AT5G44190.1	58.824	51	21	0	165	215	146	196	1.14e-13	69.3
Msa0500300	AT5G44190.2	58.824	51	21	0	165	215	146	196	1.15e-13	69.3
Msa0500300	AT5G07210.2	31.373	153	93	4	46	196	16	158	1.66e-13	69.3
Msa0500300	AT5G59570.2	51.923	52	25	0	164	215	139	190	2.28e-12	65.1
Msa0500300	AT5G59570.1	51.923	52	25	0	164	215	139	190	2.28e-12	65.1
Msa0500300	AT2G40970.1	50.980	51	25	0	165	215	104	154	2.62e-12	64.7
Msa0500300	AT4G18020.8	35.385	130	75	3	40	162	12	139	2.65e-12	65.9
Msa0500300	AT4G18020.11	35.385	130	75	3	40	162	12	139	2.65e-12	65.9
Msa0500300	AT4G18020.4	35.385	130	75	3	40	162	12	139	2.65e-12	65.9
Msa0500300	AT4G18020.5	35.385	130	75	3	40	162	12	139	2.65e-12	65.9
Msa0500300	AT4G18020.10	35.385	130	75	3	40	162	12	139	2.65e-12	65.9
Msa0500300	AT4G18020.7	35.385	130	75	3	40	162	12	139	2.65e-12	65.9
Msa0500300	AT3G46640.2	52.941	51	24	0	165	215	143	193	2.71e-12	65.1
Msa0500300	AT3G46640.1	52.941	51	24	0	165	215	143	193	2.71e-12	65.1
Msa0500300	AT3G46640.3	52.941	51	24	0	165	215	143	193	2.91e-12	65.1
Msa0500300	AT4G18020.3	35.385	130	75	3	40	162	12	139	2.94e-12	65.5
Msa0500300	AT4G18020.2	35.385	130	75	3	40	162	12	139	2.94e-12	65.5
Msa0500300	AT4G18020.1	35.385	130	75	3	40	162	12	139	2.94e-12	65.5
Msa0500300	AT4G18020.6	35.385	130	75	3	40	162	12	139	2.94e-12	65.5
Msa0500300	AT4G18020.9	35.385	130	75	3	40	162	12	139	2.94e-12	65.5
Msa0500300	AT5G07210.1	30.488	164	88	6	46	196	16	166	4.71e-12	65.1
Msa0500300	AT2G20570.1	56.863	51	22	0	165	215	152	202	5.04e-12	64.7
Msa0500300	AT2G20570.2	62.222	45	17	0	171	215	174	218	8.58e-12	63.9
Msa0500300	AT5G05090.1	49.020	51	26	0	165	215	80	130	2.06e-11	62.4
Msa0500300	AT3G10760.1	49.020	51	26	0	165	215	104	154	2.70e-11	62.4

Find 79 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCTCCTTCTCTCTTGCTTT+TGG	0.205158	3_4:-40593124	Msa0500300:CDS
CGGGCTATATTCAGATAATT+TGG	0.210547	3_4:+40589833	None:intergenic
AAGGTCTTCAATATTATTTA+TGG	0.259242	3_4:+40591770	None:intergenic
CTTGAAACTGTTAATCAGTT+AGG	0.311031	3_4:-40592363	Msa0500300:CDS
CTGAAAAGAATGGATGGTTC+TGG	0.324289	3_4:-40592071	Msa0500300:three_prime_UTR
ACAGATGGCATGTCCTTGTT+AGG	0.347468	3_4:-40591856	Msa0500300:three_prime_UTR
CGTTAATCAGAATATACTTC+AGG	0.360139	3_4:-40591958	Msa0500300:three_prime_UTR
CTGAAGTATATTCTGATTAA+CGG	0.378117	3_4:+40591959	None:intergenic
CCTCTCATACATAGAATTTG+TGG	0.379490	3_4:+40591810	None:intergenic
GCTCCACAGACCAAACAAAC+TGG	0.386812	3_4:+40592400	None:intergenic
TCTGCTGAAACCTGTGAGAT+TGG	0.389884	3_4:-40592469	Msa0500300:CDS
TGGAGGAACTAAGGAACATT+TGG	0.394946	3_4:-40592449	Msa0500300:CDS
ATAAGAAGTCCCAGTTTGTT+TGG	0.408211	3_4:-40592410	Msa0500300:CDS
TAGTAGAGAAGAGAGAATAA+TGG	0.426818	3_4:-40593202	Msa0500300:CDS
ATTATTCTCTCTTCTCTACT+AGG	0.429390	3_4:+40593204	None:intergenic
AACACTCAGCTGGTCTGAAC+GGG	0.433884	3_4:-40591990	Msa0500300:three_prime_UTR
ACATTTGGCAGCATGTAATT+CGG	0.438242	3_4:-40592434	Msa0500300:CDS
TACTTCAGGGAATGTCCATA+TGG	0.451003	3_4:-40591944	Msa0500300:three_prime_UTR
TGTTATTGCTTTACTAGTTG+TGG	0.457990	3_4:+40592808	None:intergenic
GGAACTTGTAGGACTTGAAA+TGG	0.458110	3_4:-40592696	Msa0500300:CDS
AGCTGGTCTGAACGGGCAGC+TGG	0.466082	3_4:-40591983	Msa0500300:three_prime_UTR
AGCTTGAGTCCATCCATGTC+AGG	0.472593	3_4:+40592722	None:intergenic
AACCATGCCACTAGGTGAAA+AGG	0.475002	3_4:+40592022	None:intergenic
CAGAAAATCACGGGCACCAT+GGG	0.476586	3_4:+40592488	None:intergenic
TGAGAAAACCATCTCTGTTA+AGG	0.477191	3_4:+40593623	None:intergenic
TTGCAAGTGATCAAACCATA+TGG	0.480135	3_4:+40591929	None:intergenic
AGACGTGGCGACCCATCTCC+AGG	0.480850	3_4:-40592194	Msa0500300:CDS
CTTAGTTCCTCCAATCTCAC+AGG	0.481151	3_4:+40592459	None:intergenic
TAGACTCTTTCTGAAAAGAA+TGG	0.483472	3_4:-40592081	Msa0500300:three_prime_UTR
CTGTGAGATTGGAGGAACTA+AGG	0.487418	3_4:-40592458	Msa0500300:CDS
ACTAGAAAAGTGCCTGGAGA+TGG	0.493120	3_4:+40592182	None:intergenic
GTCCCAGTTTGTTTGGTCTG+TGG	0.493912	3_4:-40592403	Msa0500300:CDS
CTCAAGCTGCTGGAACTTGT+AGG	0.500097	3_4:-40592707	Msa0500300:CDS
TGATACGGAGCTGGTGATGA+AGG	0.507591	3_4:-40592517	Msa0500300:CDS
CAAAACTTTGCAACCTAACA+AGG	0.511241	3_4:+40591843	None:intergenic
CATGGATGGACTCAAGCTGC+TGG	0.519635	3_4:-40592717	Msa0500300:CDS
GTTAATCAGAATATACTTCA+GGG	0.520670	3_4:-40591957	Msa0500300:three_prime_UTR
TGTGAACTTCAGCGATTACC+AGG	0.522457	3_4:+40592747	None:intergenic
CTAGAAAAGTGCCTGGAGAT+GGG	0.532146	3_4:+40592183	None:intergenic
CTCGCCAACTTACTTATGAC+AGG	0.542488	3_4:+40592668	None:intergenic
CACAAATTCTATGTATGAGA+GGG	0.544290	3_4:-40591809	Msa0500300:three_prime_UTR
CAGATAATTTGGAGGGAGCA+AGG	0.544768	3_4:+40589844	None:intergenic
CTCCACAGACCAAACAAACT+GGG	0.546619	3_4:+40592401	None:intergenic
TCCACTGATCGGTTTCCCAT+CGG	0.547632	3_4:-40593174	Msa0500300:CDS
CTCTTTCTGAAAAGAATGGA+TGG	0.549493	3_4:-40592077	Msa0500300:three_prime_UTR
AGCGAGAACACGCATTCCGA+TGG	0.550357	3_4:+40593158	None:intergenic
TGGTAATCTGAACACTCAGC+TGG	0.550977	3_4:-40592000	Msa0500300:three_prime_UTR
TAAAGGACTAGAAAAGTGCC+TGG	0.553641	3_4:+40592176	None:intergenic
GCTATATTCAGATAATTTGG+AGG	0.554294	3_4:+40589836	None:intergenic
TGAATGTTGAAAATATTACA+AGG	0.562722	3_4:-40592219	Msa0500300:CDS
GAACACTCAGCTGGTCTGAA+CGG	0.563794	3_4:-40591991	Msa0500300:three_prime_UTR
GCTGAAACCTGTGAGATTGG+AGG	0.565413	3_4:-40592466	Msa0500300:CDS
CCACAAATTCTATGTATGAG+AGG	0.570704	3_4:-40591810	Msa0500300:three_prime_UTR
TCCAAAAGCAAGAGAGAAGG+AGG	0.572741	3_4:+40593123	None:intergenic
GTTGTCTGTATATAGTGATA+CGG	0.576810	3_4:-40592532	Msa0500300:CDS
AGATCGTGCCTTAACAGAGA+TGG	0.578031	3_4:-40593631	Msa0500300:intron
GACCGTGGTATTGACATGAT+CGG	0.585397	3_4:+40593091	None:intergenic
CAGGTTTCAGCAGAAAATCA+CGG	0.587467	3_4:+40592478	None:intergenic
TAACAGTTTCAAGAAACTTG+CGG	0.590533	3_4:+40592373	None:intergenic
GTTTCCAAAAGCAAGAGAGA+AGG	0.592241	3_4:+40593120	None:intergenic
GCAGAAAATCACGGGCACCA+TGG	0.594928	3_4:+40592487	None:intergenic
TGTATATAGTGATACGGAGC+TGG	0.596919	3_4:-40592526	Msa0500300:CDS
TAATGGATGATTCCACTGAT+CGG	0.598611	3_4:-40593185	Msa0500300:CDS
AGATTACCAACCATGCCACT+AGG	0.600367	3_4:+40592014	None:intergenic
TCCGATGGGAAACCGATCAG+TGG	0.602032	3_4:+40593173	None:intergenic
AGGTTTCAGCAGAAAATCAC+GGG	0.606322	3_4:+40592479	None:intergenic
TGATCACTTGCAAAACAGCA+AGG	0.610848	3_4:-40591919	Msa0500300:three_prime_UTR
GCGAGAACACGCATTCCGAT+GGG	0.611220	3_4:+40593159	None:intergenic
TACTAGGCACAAACTCAGAA+CGG	0.611344	3_4:+40593220	None:intergenic
GTTCACATGCCTGACATGGA+TGG	0.616361	3_4:-40592731	Msa0500300:CDS
CTATATTCAGATAATTTGGA+GGG	0.621088	3_4:+40589837	None:intergenic
GTGATGAAGGCAATTTCCCA+TGG	0.624988	3_4:-40592504	Msa0500300:CDS
TGAAGTTCACATGCCTGACA+TGG	0.628648	3_4:-40592735	Msa0500300:CDS
GAATGTTGAAAATATTACAA+GGG	0.643931	3_4:-40592218	Msa0500300:CDS
AAATATTACAAGGGAAGACG+TGG	0.654849	3_4:-40592209	Msa0500300:CDS
AAACATAGACATGTTTGACC+TGG	0.663608	3_4:-40592765	Msa0500300:CDS
GTAAATATTAAAACAGAACA+AGG	0.664926	3_4:+40591751	None:intergenic
CGTGGTATTGACATGATCGG+AGG	0.677686	3_4:+40593094	None:intergenic
CTCCGATCATGTCAATACCA+CGG	0.680497	3_4:-40593093	Msa0500300:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AACAATCTTTATTTTTTTTA+AGG	-	chr3_4:40590185-40590204	Msa0500300:intron	10.0%
!!!	AAAATAAAGATTGTTTTTAG+TGG	+	chr3_4:40590180-40590199	None:intergenic	15.0%
!!!	ATAAATAAAGATTGTTTTAG+TGG	+	chr3_4:40590236-40590255	None:intergenic	15.0%
!!!	ATATCTATCTTATTTGTTTA+TGG	-	chr3_4:40590648-40590667	Msa0500300:intron	15.0%
!!	TTATAAAAATTAAAGCGTAA+AGG	+	chr3_4:40591339-40591358	None:intergenic	15.0%
!!	GTACAAAGTATAATGAAAAT+TGG	+	chr3_4:40590496-40590515	None:intergenic	20.0%
!!!	TATCTTATTTGTTTATGGTT+TGG	-	chr3_4:40590653-40590672	Msa0500300:intron	20.0%
!!!	ATCTTATTTGTTTATGGTTT+GGG	-	chr3_4:40590654-40590673	Msa0500300:intron	20.0%
!!!	ATCAAACCAAAAATCTTTTT+GGG	+	chr3_4:40591257-40591276	None:intergenic	20.0%
!!	TGAATGTTGAAAATATTACA+AGG	-	chr3_4:40591276-40591295	Msa0500300:intron	20.0%
!!	GAATGTTGAAAATATTACAA+GGG	-	chr3_4:40591277-40591296	Msa0500300:intron	20.0%
!!	AAGGTCTTCAATATTATTTA+TGG	+	chr3_4:40591728-40591747	None:intergenic	20.0%
!!	GTAAATATTAAAACAGAACA+AGG	+	chr3_4:40591747-40591766	None:intergenic	20.0%
!!!	GGTTTTATTTCAAGATTTTA+GGG	+	chr3_4:40592026-40592045	None:intergenic	20.0%
!!!	TGGTTTTATTTCAAGATTTT+AGG	+	chr3_4:40592027-40592046	None:intergenic	20.0%
!!	AAATCTTGAAATAAAACCAA+TGG	-	chr3_4:40592028-40592047	Msa0500300:three_prime_UTR	20.0%
!!	AATCTTGAAATAAAACCAAT+GGG	-	chr3_4:40592029-40592048	Msa0500300:three_prime_UTR	20.0%
!!!	GGATTGTTGAATTATTTTTT+GGG	+	chr3_4:40592829-40592848	None:intergenic	20.0%
!!!	CTATAAAAAAGGTATTCATT+TGG	+	chr3_4:40592959-40592978	None:intergenic	20.0%
!!	TCTTATTAAAAATAAGACGT+GGG	+	chr3_4:40593433-40593452	None:intergenic	20.0%
!!!	TAGTCATGTAATTTTATTTG+AGG	-	chr3_4:40593495-40593514	Msa0500300:intron	20.0%
!	TCGTTTCTATATATAGGTAT+TGG	-	chr3_4:40589951-40589970	Msa0500300:intron	25.0%
!!!	TCTATATATAGGTATTGGAT+TGG	-	chr3_4:40589956-40589975	Msa0500300:intron	25.0%
!!!	TTAGTCTTATACACGTAAAT+TGG	+	chr3_4:40590453-40590472	None:intergenic	25.0%
!	AATGAAGAAACAGAACAAAT+AGG	+	chr3_4:40590878-40590897	None:intergenic	25.0%
!	GATCGTTGTTATAAATTGTT+TGG	-	chr3_4:40590933-40590952	Msa0500300:intron	25.0%
!!!	TCATGTTAATGTTGTTTTAC+TGG	-	chr3_4:40591220-40591239	Msa0500300:intron	25.0%
!!	CATCAAACCAAAAATCTTTT+TGG	+	chr3_4:40591258-40591277	None:intergenic	25.0%
!!!	ATGTTTTCTGGTTTATGAAA+TGG	-	chr3_4:40591380-40591399	Msa0500300:intron	25.0%
!	CTGAAGTATATTCTGATTAA+CGG	+	chr3_4:40591539-40591558	None:intergenic	25.0%
!	GTTAATCAGAATATACTTCA+GGG	-	chr3_4:40591538-40591557	Msa0500300:intron	25.0%
!	GTACTCATATTATCAATGAA+AGG	+	chr3_4:40591813-40591832	None:intergenic	25.0%
!!!	GGGATTGTTGAATTATTTTT+TGG	+	chr3_4:40592830-40592849	None:intergenic	25.0%
!	TCTCTCTTCATCTATAAAAA+AGG	+	chr3_4:40592970-40592989	None:intergenic	25.0%
!	TCCAATAGAAGAAACATAAT+CGG	-	chr3_4:40593014-40593033	Msa0500300:intron	25.0%
!	TTAATGTTATTCGTTCTATG+TGG	+	chr3_4:40593386-40593405	None:intergenic	25.0%
!	CTCTTATTAAAAATAAGACG+TGG	+	chr3_4:40593434-40593453	None:intergenic	25.0%
!	CTATATTCAGATAATTTGGA+GGG	+	chr3_4:40593661-40593680	None:intergenic	25.0%
	TAGTGCTCGTTTCTATATAT+AGG	-	chr3_4:40589945-40589964	Msa0500300:intron	30.0%
	TATTATCGTTGGAGGTAAAA+TGG	+	chr3_4:40590077-40590096	None:intergenic	30.0%
	TTTACCTCCAACGATAATAA+CGG	-	chr3_4:40590078-40590097	Msa0500300:intron	30.0%
	TAAACAACCGTTATTATCGT+TGG	+	chr3_4:40590088-40590107	None:intergenic	30.0%
!	TCTTAACCAATTTTTGCGAA+CGG	+	chr3_4:40590132-40590151	None:intergenic	30.0%
	ATTATTCTCTCTTCTCTACT+AGG	+	chr3_4:40590294-40590313	None:intergenic	30.0%
	TAGTAGAGAAGAGAGAATAA+TGG	-	chr3_4:40590293-40590312	Msa0500300:intron	30.0%
	TGTTATTGCTTTACTAGTTG+TGG	+	chr3_4:40590690-40590709	None:intergenic	30.0%
	GTTGTCTGTATATAGTGATA+CGG	-	chr3_4:40590963-40590982	Msa0500300:intron	30.0%
	TAACAGTTTCAAGAAACTTG+CGG	+	chr3_4:40591125-40591144	None:intergenic	30.0%
	CTTGAAACTGTTAATCAGTT+AGG	-	chr3_4:40591132-40591151	Msa0500300:intron	30.0%
!!!	TTTTTTCGCTGAATGTTTTC+TGG	-	chr3_4:40591368-40591387	Msa0500300:intron	30.0%
!	TAGACTCTTTCTGAAAAGAA+TGG	-	chr3_4:40591414-40591433	Msa0500300:intron	30.0%
	CGTTAATCAGAATATACTTC+AGG	-	chr3_4:40591537-40591556	Msa0500300:intron	30.0%
!	TGTCTAAAACATTCACTTGA+GGG	+	chr3_4:40591622-40591641	None:intergenic	30.0%
!!	AAGTGAATGTTTTAGACAGA+TGG	-	chr3_4:40591624-40591643	Msa0500300:intron	30.0%
	CACAAATTCTATGTATGAGA+GGG	-	chr3_4:40591686-40591705	Msa0500300:intron	30.0%
	TATTAGATGCTTGAGACTAT+AGG	-	chr3_4:40591773-40591792	Msa0500300:three_prime_UTR	30.0%
	ATTGCAGATATCTTCACTAA+GGG	+	chr3_4:40592058-40592077	None:intergenic	30.0%
	AATTGCAGATATCTTCACTA+AGG	+	chr3_4:40592059-40592078	None:intergenic	30.0%
	AGATATCTGCAATTTGATAG+CGG	-	chr3_4:40592065-40592084	Msa0500300:three_prime_UTR	30.0%
	TTTATTTATGCACAATCCGA+TGG	+	chr3_4:40592560-40592579	None:intergenic	30.0%
!	ATCTTTGGTTTAGTATCAAC+CGG	-	chr3_4:40592697-40592716	Msa0500300:CDS	30.0%
!!	GAGTTTGCTATGAAAGATTT+GGG	+	chr3_4:40592850-40592869	None:intergenic	30.0%
!!	TGAGTTTGCTATGAAAGATT+TGG	+	chr3_4:40592851-40592870	None:intergenic	30.0%
	TGATATCATCTACATACAGT+AGG	-	chr3_4:40592926-40592945	Msa0500300:intron	30.0%
	TTCTGCGAAAATCATTGTAT+GGG	+	chr3_4:40593077-40593096	None:intergenic	30.0%
	AGGCATAAAGAAAAATCTGA+TGG	+	chr3_4:40593357-40593376	None:intergenic	30.0%
!	TTCGTTCTATGTGGATTTTT+AGG	+	chr3_4:40593377-40593396	None:intergenic	30.0%
	CGAATAACATTAACATCATG+AGG	-	chr3_4:40593393-40593412	Msa0500300:intron	30.0%
	TAACATTAACATCATGAGGA+CGG	-	chr3_4:40593397-40593416	Msa0500300:intron	30.0%
	CTAAGTAACACATTGGAAAA+TGG	+	chr3_4:40593478-40593497	None:intergenic	30.0%
	TACATGACTAAGTAACACAT+TGG	+	chr3_4:40591950-40591969	None:intergenic	30.0%
	TTGTATCCATGATGATTTGA+TGG	-	chr3_4:40593532-40593551	Msa0500300:intron	30.0%
!	ACTTAGTCATGTGTCTTTTT+AGG	+	chr3_4:40593562-40593581	None:intergenic	30.0%
	GCTATATTCAGATAATTTGG+AGG	+	chr3_4:40593662-40593681	None:intergenic	30.0%
	TGAGAAAACCATCTCTGTTA+AGG	+	chr3_4:40589875-40589894	None:intergenic	35.0%
!	TTAACAGAGATGGTTTTCTC+AGG	-	chr3_4:40589874-40589893	Msa0500300:intron	35.0%
!	TTTTCTCAGGTTAGGGTTTA+GGG	-	chr3_4:40589887-40589906	Msa0500300:intron	35.0%
	AATGACTGCTTATGCTCTTT+GGG	-	chr3_4:40590018-40590037	Msa0500300:intron	35.0%
	AACGGTTGTTTACTCCTTTA+TGG	-	chr3_4:40590096-40590115	Msa0500300:intron	35.0%
	ACGGTACTTAACTACCATAA+AGG	+	chr3_4:40590113-40590132	None:intergenic	35.0%
!	TAAGTACCGTTCGCAAAAAT+TGG	-	chr3_4:40590123-40590142	Msa0500300:intron	35.0%
!	TAATGGATGATTCCACTGAT+CGG	-	chr3_4:40590310-40590329	Msa0500300:intron	35.0%
	ACTTTGTACCTGTTATTGCT+TGG	-	chr3_4:40590507-40590526	Msa0500300:intron	35.0%
!	TCTGAAATCCAAGCAATAAC+AGG	+	chr3_4:40590518-40590537	None:intergenic	35.0%
	TAGACTTTGAATGCATATCC+AGG	+	chr3_4:40590610-40590629	None:intergenic	35.0%
	AAACATAGACATGTTTGACC+TGG	-	chr3_4:40590730-40590749	Msa0500300:intron	35.0%
	TCTTCCTGTCATAAGTAAGT+TGG	-	chr3_4:40590823-40590842	Msa0500300:intron	35.0%
	ACATTTGGCAGCATGTAATT+CGG	-	chr3_4:40591061-40591080	Msa0500300:intron	35.0%
!	ATAAGAAGTCCCAGTTTGTT+TGG	-	chr3_4:40591085-40591104	Msa0500300:intron	35.0%
!!!	TAATGTTGTTTTACTGGCAG+AGG	-	chr3_4:40591226-40591245	Msa0500300:intron	35.0%
!	GCTGTACCCAAAAAGATTTT+TGG	-	chr3_4:40591248-40591267	Msa0500300:intron	35.0%
	AAATATTACAAGGGAAGACG+TGG	-	chr3_4:40591286-40591305	Msa0500300:intron	35.0%
!	CTCTTTCTGAAAAGAATGGA+TGG	-	chr3_4:40591418-40591437	Msa0500300:intron	35.0%
	TTGCAAGTGATCAAACCATA+TGG	+	chr3_4:40591569-40591588	None:intergenic	35.0%
!	TTGAGGGGAAATAATTTGTC+TGG	+	chr3_4:40591606-40591625	None:intergenic	35.0%
!	GTCTAAAACATTCACTTGAG+GGG	+	chr3_4:40591621-40591640	None:intergenic	35.0%
!	CTGTCTAAAACATTCACTTG+AGG	+	chr3_4:40591623-40591642	None:intergenic	35.0%
	CAAAACTTTGCAACCTAACA+AGG	+	chr3_4:40591655-40591674	None:intergenic	35.0%
	CCTCTCATACATAGAATTTG+TGG	+	chr3_4:40591688-40591707	None:intergenic	35.0%
	CCACAAATTCTATGTATGAG+AGG	-	chr3_4:40591685-40591704	Msa0500300:intron	35.0%
!	GATATCTGCAATTTGATAGC+GGG	-	chr3_4:40592066-40592085	Msa0500300:three_prime_UTR	35.0%
	GTTAGTGCCATATATCTATC+CGG	+	chr3_4:40592193-40592212	None:intergenic	35.0%
!!	AACTAAAGTAGCCTTGTGAA+TGG	-	chr3_4:40592225-40592244	Msa0500300:CDS	35.0%
!!	ACTAAAGTAGCCTTGTGAAT+GGG	-	chr3_4:40592226-40592245	Msa0500300:CDS	35.0%
!!	TTGGTTACGAAACTTGCTTT+TGG	+	chr3_4:40592263-40592282	None:intergenic	35.0%
	ATGTGGTTTCTGAATCTTGT+TGG	+	chr3_4:40592282-40592301	None:intergenic	35.0%
	ACAAGATTCAGAAACCACAT+TGG	-	chr3_4:40592282-40592301	Msa0500300:intron	35.0%
	AGATAAAGTAGGTTGTCGTT+TGG	-	chr3_4:40592339-40592358	Msa0500300:intron	35.0%
	TCTGGTTACTGTGTATTTCT+TGG	+	chr3_4:40592388-40592407	None:intergenic	35.0%
	AAGAAATACACAGTAACCAG+AGG	-	chr3_4:40592387-40592406	Msa0500300:CDS	35.0%
	TGCACTGCATAAGAAATATC+AGG	-	chr3_4:40592589-40592608	Msa0500300:intron	35.0%
	ATAGAGAAGGGTCTTCAAAT+AGG	-	chr3_4:40592656-40592675	Msa0500300:intron	35.0%
	TAGAGAAGGGTCTTCAAATA+GGG	-	chr3_4:40592657-40592676	Msa0500300:intron	35.0%
!!!	TTCAAATAGGGTGCTACTTT+TGG	-	chr3_4:40592669-40592688	Msa0500300:intron	35.0%
	AATTATTGAACGTGCTGGAA+TGG	+	chr3_4:40592746-40592765	None:intergenic	35.0%
	GAAGAAATTATTGAACGTGC+TGG	+	chr3_4:40592751-40592770	None:intergenic	35.0%
	TGAGACAAAAATAATCCACC+AGG	-	chr3_4:40592784-40592803	Msa0500300:CDS	35.0%
	GTGATATGATAGACTTTCGA+AGG	-	chr3_4:40592881-40592900	Msa0500300:intron	35.0%
	TATCATCTACATACAGTAGG+AGG	-	chr3_4:40592929-40592948	Msa0500300:intron	35.0%
	GCCGATTATGTTTCTTCTAT+TGG	+	chr3_4:40593018-40593037	None:intergenic	35.0%
	CTTCTGCGAAAATCATTGTA+TGG	+	chr3_4:40593078-40593097	None:intergenic	35.0%
	GTATACATGCATCAACCATT+AGG	+	chr3_4:40593135-40593154	None:intergenic	35.0%
!	TGAAGAATGCATTTTTGCAC+GGG	+	chr3_4:40593173-40593192	None:intergenic	35.0%
!	GTGAAGAATGCATTTTTGCA+CGG	+	chr3_4:40593174-40593193	None:intergenic	35.0%
!!	CATTCTTCACATCAAGTTGA+TGG	-	chr3_4:40593184-40593203	Msa0500300:CDS	35.0%
!!	TTCACATCAAGTTGATGGAT+TGG	-	chr3_4:40593189-40593208	Msa0500300:CDS	35.0%
	GGATAAAGCAAGACTAAGAA+CGG	-	chr3_4:40593221-40593240	Msa0500300:CDS	35.0%
!	AATCTGATGGTTCTTTTGAG+CGG	+	chr3_4:40593344-40593363	None:intergenic	35.0%
	AACATTAACATCATGAGGAC+GGG	-	chr3_4:40593398-40593417	Msa0500300:intron	35.0%
	AACACATTGGAAAATGGCTA+TGG	+	chr3_4:40593472-40593491	None:intergenic	35.0%
	GGATATCCATCAAATCATCA+TGG	+	chr3_4:40593541-40593560	None:intergenic	35.0%
	CGGGCTATATTCAGATAATT+TGG	+	chr3_4:40593665-40593684	None:intergenic	35.0%
!	AGAGATGGTTTTCTCAGGTT+AGG	-	chr3_4:40589879-40589898	Msa0500300:intron	40.0%
!	GAGATGGTTTTCTCAGGTTA+GGG	-	chr3_4:40589880-40589899	Msa0500300:intron	40.0%
!	GTTTTCTCAGGTTAGGGTTT+AGG	-	chr3_4:40589886-40589905	Msa0500300:intron	40.0%
	TTTAGGGAAGTTGCTATCGA+TGG	-	chr3_4:40589903-40589922	Msa0500300:intron	40.0%
!	CAAAGAGCATAAGCAGTCAT+TGG	+	chr3_4:40590019-40590038	None:intergenic	40.0%
	CAATGACTGCTTATGCTCTT+TGG	-	chr3_4:40590017-40590036	Msa0500300:intron	40.0%
	ACAACCGTTATTATCGTTGG+AGG	+	chr3_4:40590085-40590104	None:intergenic	40.0%
	TACTAGGCACAAACTCAGAA+CGG	+	chr3_4:40590278-40590297	None:intergenic	40.0%
	GTTTCCAAAAGCAAGAGAGA+AGG	+	chr3_4:40590378-40590397	None:intergenic	40.0%
	TATCAAAAAGAAGCAGACCG+TGG	+	chr3_4:40590422-40590441	None:intergenic	40.0%
	GGAACTTGTAGGACTTGAAA+TGG	-	chr3_4:40590799-40590818	Msa0500300:intron	40.0%
	TGTATATAGTGATACGGAGC+TGG	-	chr3_4:40590969-40590988	Msa0500300:intron	40.0%
	AGGTTTCAGCAGAAAATCAC+GGG	+	chr3_4:40591019-40591038	None:intergenic	40.0%
	CAGGTTTCAGCAGAAAATCA+CGG	+	chr3_4:40591020-40591039	None:intergenic	40.0%
	TGGAGGAACTAAGGAACATT+TGG	-	chr3_4:40591046-40591065	Msa0500300:intron	40.0%
!!	TAAAGGACTAGAAAAGTGCC+TGG	+	chr3_4:40591322-40591341	None:intergenic	40.0%
	CTGAAAAGAATGGATGGTTC+TGG	-	chr3_4:40591424-40591443	Msa0500300:intron	40.0%
!	TTCAGATCCTTTTCACCTAG+TGG	-	chr3_4:40591466-40591485	Msa0500300:intron	40.0%
	TACTTCAGGGAATGTCCATA+TGG	-	chr3_4:40591551-40591570	Msa0500300:intron	40.0%
	TGATCACTTGCAAAACAGCA+AGG	-	chr3_4:40591576-40591595	Msa0500300:intron	40.0%
	GCGCACAAAACATATCGAAA+TGG	+	chr3_4:40592152-40592171	None:intergenic	40.0%
!	TTTCGATATGTTTTGTGCGC+TGG	-	chr3_4:40592152-40592171	Msa0500300:three_prime_UTR	40.0%
	GGGATTTCCGGATAGATATA+TGG	-	chr3_4:40592183-40592202	Msa0500300:CDS	40.0%
!!	CTAAAGTAGCCTTGTGAATG+GGG	-	chr3_4:40592227-40592246	Msa0500300:CDS	40.0%
	ATTACATGTCCCCATTCACA+AGG	+	chr3_4:40592239-40592258	None:intergenic	40.0%
	GCACTTGAACGAGATAAAGT+AGG	-	chr3_4:40592328-40592347	Msa0500300:intron	40.0%
!	TTGGTGACAACTTACTCTCA+TGG	+	chr3_4:40592369-40592388	None:intergenic	40.0%
	AAATACACAGTAACCAGAGG+TGG	-	chr3_4:40592390-40592409	Msa0500300:CDS	40.0%
	TGCTTACACAGATGCAGATT+GGG	+	chr3_4:40592440-40592459	None:intergenic	40.0%
	TTGCTTACACAGATGCAGAT+TGG	+	chr3_4:40592441-40592460	None:intergenic	40.0%
	GTGTGCCTTGAATATATCTC+AGG	-	chr3_4:40592506-40592525	Msa0500300:CDS	40.0%
	CGCATCCTGAGATATATTCA+AGG	+	chr3_4:40592514-40592533	None:intergenic	40.0%
!	AGAAATATCAGGCCTTGTGA+AGG	-	chr3_4:40592600-40592619	Msa0500300:intron	40.0%
	TTGTAAACCATGTCCTACAC+CGG	+	chr3_4:40592719-40592738	None:intergenic	40.0%
	TCATAGCAAACTCAGAGCTA+AGG	-	chr3_4:40592857-40592876	Msa0500300:intron	40.0%
	ATCTACATACAGTAGGAGGT+AGG	-	chr3_4:40592933-40592952	Msa0500300:intron	40.0%
	GCATCAACCATTAGGTTTCA+AGG	+	chr3_4:40593127-40593146	None:intergenic	40.0%
!	GAAGAATGCATTTTTGCACG+GGG	+	chr3_4:40593172-40593191	None:intergenic	40.0%
	GTCTTGCTTTATCCAAAGCA+TGG	+	chr3_4:40593215-40593234	None:intergenic	40.0%
	AAGACTAAGAACGGTTCGAA+TGG	-	chr3_4:40593230-40593249	Msa0500300:CDS	40.0%
	ACATTAACATCATGAGGACG+GGG	-	chr3_4:40593399-40593418	Msa0500300:intron	40.0%
	AGATCGTGCCTTAACAGAGA+TGG	-	chr3_4:40589864-40589883	Msa0500300:intron	45.0%
!!	AATGACGCCGCTGACTTATT+GGG	-	chr3_4:40589979-40589998	Msa0500300:intron	45.0%
	AGTACGACCCAATAAGTCAG+CGG	+	chr3_4:40589989-40590008	None:intergenic	45.0%
	ACTTATTGGGTCGTACTCTG+TGG	-	chr3_4:40589992-40590011	Msa0500300:intron	45.0%
	CTTATTGGGTCGTACTCTGT+GGG	-	chr3_4:40589993-40590012	Msa0500300:intron	45.0%
!	TCCTCCTTCTCTCTTGCTTT+TGG	-	chr3_4:40590371-40590390	Msa0500300:intron	45.0%
	TCCAAAAGCAAGAGAGAAGG+AGG	+	chr3_4:40590375-40590394	None:intergenic	45.0%
	CTCCGATCATGTCAATACCA+CGG	-	chr3_4:40590402-40590421	Msa0500300:intron	45.0%
!!	GACCGTGGTATTGACATGAT+CGG	+	chr3_4:40590407-40590426	None:intergenic	45.0%
	ATTGCTTTCTGTTCTGCTCC+TGG	-	chr3_4:40590589-40590608	Msa0500300:intron	45.0%
	TGTGAACTTCAGCGATTACC+AGG	+	chr3_4:40590751-40590770	None:intergenic	45.0%
	TGAAGTTCACATGCCTGACA+TGG	-	chr3_4:40590760-40590779	Msa0500300:intron	45.0%
	CTCGCCAACTTACTTATGAC+AGG	+	chr3_4:40590830-40590849	None:intergenic	45.0%
	GTGATGAAGGCAATTTCCCA+TGG	-	chr3_4:40590991-40591010	Msa0500300:intron	45.0%
	TCTGCTGAAACCTGTGAGAT+TGG	-	chr3_4:40591026-40591045	Msa0500300:intron	45.0%
	CTTAGTTCCTCCAATCTCAC+AGG	+	chr3_4:40591039-40591058	None:intergenic	45.0%
	CTGTGAGATTGGAGGAACTA+AGG	-	chr3_4:40591037-40591056	Msa0500300:intron	45.0%
	CTCCACAGACCAAACAAACT+GGG	+	chr3_4:40591097-40591116	None:intergenic	45.0%
!!	CTAGAAAAGTGCCTGGAGAT+GGG	+	chr3_4:40591315-40591334	None:intergenic	45.0%
!!	ACTAGAAAAGTGCCTGGAGA+TGG	+	chr3_4:40591316-40591335	None:intergenic	45.0%
!	ATCCTTTTCACCTAGTGGCA+TGG	-	chr3_4:40591471-40591490	Msa0500300:intron	45.0%
	AACCATGCCACTAGGTGAAA+AGG	+	chr3_4:40591476-40591495	None:intergenic	45.0%
!	TTTTCACCTAGTGGCATGGT+TGG	-	chr3_4:40591475-40591494	Msa0500300:intron	45.0%
	AGATTACCAACCATGCCACT+AGG	+	chr3_4:40591484-40591503	None:intergenic	45.0%
	TGGTAATCTGAACACTCAGC+TGG	-	chr3_4:40591495-40591514	Msa0500300:intron	45.0%
	ACAGATGGCATGTCCTTGTT+AGG	-	chr3_4:40591639-40591658	Msa0500300:intron	45.0%
!!	TCTGCAATTTGATAGCGGGA+TGG	-	chr3_4:40592070-40592089	Msa0500300:three_prime_UTR	45.0%
	GTTCGTGAAAAAGTAGCTCG+TGG	+	chr3_4:40592118-40592137	None:intergenic	45.0%
	ATATCGAGGAGTTGCCAATG+TGG	+	chr3_4:40592299-40592318	None:intergenic	45.0%
!	AGTGCTGAAGCAGAATATCG+AGG	+	chr3_4:40592313-40592332	None:intergenic	45.0%
	GCTTACACAGATGCAGATTG+GGG	+	chr3_4:40592439-40592458	None:intergenic	45.0%
	ATTCAAGGCACACGTGAGTA+TGG	+	chr3_4:40592499-40592518	None:intergenic	45.0%
	TATATCTCAGGATGCGTCGA+AGG	-	chr3_4:40592518-40592537	Msa0500300:CDS	45.0%
	ATATCTCAGGATGCGTCGAA+GGG	-	chr3_4:40592519-40592538	Msa0500300:CDS	45.0%
	TACAGTACCTCACCTTCACA+AGG	+	chr3_4:40592615-40592634	None:intergenic	45.0%
!!	TTGTGAAGGTGAGGTACTGT+AGG	-	chr3_4:40592614-40592633	Msa0500300:intron	45.0%
!!	TGTGAAGGTGAGGTACTGTA+GGG	-	chr3_4:40592615-40592634	Msa0500300:intron	45.0%
!!	CTACTTTTGGCGCTCATCTT+TGG	-	chr3_4:40592682-40592701	Msa0500300:CDS	45.0%
	GGTTTAGTATCAACCGGTGT+AGG	-	chr3_4:40592703-40592722	Msa0500300:CDS	45.0%
	AGACTTTCGAAGGTCATCAG+AGG	-	chr3_4:40592891-40592910	Msa0500300:intron	45.0%
	CGCAGAAGACATACATGATG+TGG	-	chr3_4:40593090-40593109	Msa0500300:intron	45.0%
	ATACATGATGTGGACGCTCA+GGG	-	chr3_4:40593100-40593119	Msa0500300:CDS	45.0%
	TCAGGGTCCTTGAAACCTAA+TGG	-	chr3_4:40593117-40593136	Msa0500300:CDS	45.0%
!!	TTGATGGATTGGCCATGCTT+TGG	-	chr3_4:40593200-40593219	Msa0500300:CDS	45.0%
!!	ATGGTTGCCGGTTTAACAAC+TGG	-	chr3_4:40593249-40593268	Msa0500300:intron	45.0%
!!	TGGTTGCCGGTTTAACAACT+GGG	-	chr3_4:40593250-40593269	Msa0500300:intron	45.0%
	GTTCAGCCCAGTTGTTAAAC+CGG	+	chr3_4:40593259-40593278	None:intergenic	45.0%
!!	CAACAGGTTGGTGTAGATTG+TGG	+	chr3_4:40593288-40593307	None:intergenic	45.0%
	CAGATAATTTGGAGGGAGCA+AGG	+	chr3_4:40593654-40593673	None:intergenic	45.0%
!!	ATTATTAAAAGAATATTAAT+AGG	+	chr3_4:40591925-40591944	None:intergenic	5.0%
!	TGGTGATTTTCGCAGAGGAG+CGG	-	chr3_4:40589833-40589852	Msa0500300:three_prime_UTR	50.0%
	TTGCTATCGATGGAACGCAG+TGG	-	chr3_4:40589913-40589932	Msa0500300:intron	50.0%
!!	GAATGACGCCGCTGACTTAT+TGG	-	chr3_4:40589978-40589997	Msa0500300:intron	50.0%
!	TCCACTGATCGGTTTCCCAT+CGG	-	chr3_4:40590321-40590340	Msa0500300:intron	50.0%
!!	CGTGGTATTGACATGATCGG+AGG	+	chr3_4:40590404-40590423	None:intergenic	50.0%
	GTTCACATGCCTGACATGGA+TGG	-	chr3_4:40590764-40590783	Msa0500300:intron	50.0%
	AGCTTGAGTCCATCCATGTC+AGG	+	chr3_4:40590776-40590795	None:intergenic	50.0%
	CTCAAGCTGCTGGAACTTGT+AGG	-	chr3_4:40590788-40590807	Msa0500300:intron	50.0%
!	TGATACGGAGCTGGTGATGA+AGG	-	chr3_4:40590978-40590997	Msa0500300:intron	50.0%
	CAGAAAATCACGGGCACCAT+GGG	+	chr3_4:40591010-40591029	None:intergenic	50.0%
	GCTGAAACCTGTGAGATTGG+AGG	-	chr3_4:40591029-40591048	Msa0500300:intron	50.0%
!	GTCCCAGTTTGTTTGGTCTG+TGG	-	chr3_4:40591092-40591111	Msa0500300:intron	50.0%
	GCTCCACAGACCAAACAAAC+TGG	+	chr3_4:40591098-40591117	None:intergenic	50.0%
!	GAACACTCAGCTGGTCTGAA+CGG	-	chr3_4:40591504-40591523	Msa0500300:intron	50.0%
!	AACACTCAGCTGGTCTGAAC+GGG	-	chr3_4:40591505-40591524	Msa0500300:intron	50.0%
	CTTCACTAAGGGTCTCCCAT+TGG	+	chr3_4:40592047-40592066	None:intergenic	50.0%
!!	GATAGCGGGATGGAACATGT+AGG	-	chr3_4:40592080-40592099	Msa0500300:three_prime_UTR	50.0%
	GGGATGGAACATGTAGGACA+CGG	-	chr3_4:40592086-40592105	Msa0500300:three_prime_UTR	50.0%
!!	TATGTTTTGTGCGCTGGTGC+TGG	-	chr3_4:40592158-40592177	Msa0500300:three_prime_UTR	50.0%
	AGGTGGATCGTCTGGTATCT+GGG	-	chr3_4:40592407-40592426	Msa0500300:CDS	50.0%
	TACACAGATGCAGATTGGGG+AGG	+	chr3_4:40592436-40592455	None:intergenic	50.0%
	GCGTGCATGTGATCATCCAT+CGG	-	chr3_4:40592541-40592560	Msa0500300:intron	50.0%
!	TATCAGGCCTTGTGAAGGTG+AGG	-	chr3_4:40592605-40592624	Msa0500300:intron	50.0%
	CGAGGCTGCGATATAGAGAA+GGG	-	chr3_4:40592644-40592663	Msa0500300:intron	50.0%
	GTATCAACCGGTGTAGGACA+TGG	-	chr3_4:40592709-40592728	Msa0500300:CDS	50.0%
	ATTGCTGTCACACGTCATCC+TGG	+	chr3_4:40592805-40592824	None:intergenic	50.0%
	AAGCGTTTGTACCATGCTCG+AGG	-	chr3_4:40593039-40593058	Msa0500300:intron	50.0%
	AGCGTTTGTACCATGCTCGA+GGG	-	chr3_4:40593040-40593059	Msa0500300:intron	50.0%
	CATACATGATGTGGACGCTC+AGG	-	chr3_4:40593099-40593118	Msa0500300:CDS	50.0%
	AGAACGGTTCGAATGGTTGC+CGG	-	chr3_4:40593237-40593256	Msa0500300:intron	50.0%
	CTGCACCATCACCTACAAGT+CGG	-	chr3_4:40593310-40593329	Msa0500300:intron	50.0%
	TGCACCATCACCTACAAGTC+GGG	-	chr3_4:40593311-40593330	Msa0500300:intron	50.0%
!	AAAGCCCGACTTGTAGGTGA+TGG	+	chr3_4:40593318-40593337	None:intergenic	50.0%
!	GATTTTCGCAGAGGAGCGGT+TGG	-	chr3_4:40589837-40589856	Msa0500300:three_prime_UTR	55.0%
	ATCGATGGAACGCAGTGGAG+TGG	-	chr3_4:40589918-40589937	Msa0500300:intron	55.0%
	TCCGATGGGAAACCGATCAG+TGG	+	chr3_4:40590325-40590344	None:intergenic	55.0%
	GCGAGAACACGCATTCCGAT+GGG	+	chr3_4:40590339-40590358	None:intergenic	55.0%
	AGCGAGAACACGCATTCCGA+TGG	+	chr3_4:40590340-40590359	None:intergenic	55.0%
!	CATGGATGGACTCAAGCTGC+TGG	-	chr3_4:40590778-40590797	Msa0500300:intron	55.0%
	GCAGAAAATCACGGGCACCA+TGG	+	chr3_4:40591011-40591030	None:intergenic	55.0%
	ACAACCTCCCGCATCAACTG+CGG	+	chr3_4:40591987-40592006	None:intergenic	55.0%
!	CAGTTGATGCGGGAGGTTGT+CGG	-	chr3_4:40591987-40592006	Msa0500300:three_prime_UTR	55.0%
!!	TTTTGTGCGCTGGTGCTGGA+CGG	-	chr3_4:40592162-40592181	Msa0500300:three_prime_UTR	55.0%
	GTAACCAGAGGTGGATCGTC+TGG	-	chr3_4:40592399-40592418	Msa0500300:CDS	55.0%
	GATACCAGACGATCCACCTC+TGG	+	chr3_4:40592406-40592425	None:intergenic	55.0%
	GAGGTGGATCGTCTGGTATC+TGG	-	chr3_4:40592406-40592425	Msa0500300:CDS	55.0%
	TCTCTATATCGCAGCCTCGC+TGG	+	chr3_4:40592643-40592662	None:intergenic	55.0%
	GCGAGGCTGCGATATAGAGA+AGG	-	chr3_4:40592643-40592662	Msa0500300:intron	55.0%
	GCGTTTGTACCATGCTCGAG+GGG	-	chr3_4:40593041-40593060	Msa0500300:intron	55.0%
	TCAAACAAGCCCCTCGAGCA+TGG	+	chr3_4:40593053-40593072	None:intergenic	55.0%
	GATGGTGCAGGTCAACAGGT+TGG	+	chr3_4:40593300-40593319	None:intergenic	55.0%
!	AGGTGATGGTGCAGGTCAAC+AGG	+	chr3_4:40593304-40593323	None:intergenic	55.0%
!	CGACTTGTAGGTGATGGTGC+AGG	+	chr3_4:40593312-40593331	None:intergenic	55.0%
!	CGGCATAAAGCCCGACTTGT+AGG	+	chr3_4:40593324-40593343	None:intergenic	55.0%
	GATGGAACGCAGTGGAGTGG+AGG	-	chr3_4:40589921-40589940	Msa0500300:intron	60.0%
!	TCACTCCCCCGCAGTTGATG+CGG	-	chr3_4:40591976-40591995	Msa0500300:three_prime_UTR	60.0%
	AACCTCCCGCATCAACTGCG+GGG	+	chr3_4:40591985-40592004	None:intergenic	60.0%
	CAACCTCCCGCATCAACTGC+GGG	+	chr3_4:40591986-40592005	None:intergenic	60.0%
!	TTTGTGCGCTGGTGCTGGAC+GGG	-	chr3_4:40592163-40592182	Msa0500300:three_prime_UTR	60.0%
!	CTGGTGCTGGACGGGATTTC+CGG	-	chr3_4:40592171-40592190	Msa0500300:exon	60.0%
	GCTGTCACACGTCATCCTGG+TGG	+	chr3_4:40592802-40592821	None:intergenic	60.0%
	AGACGTGGCGACCCATCTCC+AGG	-	chr3_4:40591301-40591320	Msa0500300:intron	65.0%
!	AGCTGGTCTGAACGGGCAGC+TGG	-	chr3_4:40591512-40591531	Msa0500300:intron	65.0%
!	CACTCCCCCGCAGTTGATGC+GGG	-	chr3_4:40591977-40591996	Msa0500300:three_prime_UTR	65.0%
	ACCTCCCGCATCAACTGCGG+GGG	+	chr3_4:40591984-40592003	None:intergenic	65.0%
!	TCCCCCGCAGTTGATGCGGG+AGG	-	chr3_4:40591980-40591999	Msa0500300:three_prime_UTR	70.0%
!	GGTACTGTAGGGCGCCAGCG+AGG	-	chr3_4:40592626-40592645	Msa0500300:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	40589832	40593685	40589832	ID=Msa0500300;Name=Msa0500300
chr3_4	mRNA	40589832	40593685	40589832	ID=Msa0500300-mRNA-1;Parent=Msa0500300;Name=Msa0500300-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0\|0.83\|0.71\|0.85\|0.66\|0.57\|7\|455\|218
chr3_4	exon	40589832	40589865	40589832	ID=Msa0500300-mRNA-1:exon:8315;Parent=Msa0500300-mRNA-1
chr3_4	exon	40591739	40591890	40591739	ID=Msa0500300-mRNA-1:exon:8314;Parent=Msa0500300-mRNA-1
chr3_4	exon	40591914	40592271	40591914	ID=Msa0500300-mRNA-1:exon:8313;Parent=Msa0500300-mRNA-1
chr3_4	exon	40592355	40592555	40592355	ID=Msa0500300-mRNA-1:exon:8312;Parent=Msa0500300-mRNA-1
chr3_4	exon	40592681	40592833	40592681	ID=Msa0500300-mRNA-1:exon:8311;Parent=Msa0500300-mRNA-1
chr3_4	exon	40593094	40593253	40593094	ID=Msa0500300-mRNA-1:exon:8310;Parent=Msa0500300-mRNA-1
chr3_4	exon	40593632	40593685	40593632	ID=Msa0500300-mRNA-1:exon:8309;Parent=Msa0500300-mRNA-1
chr3_4	CDS	40593632	40593685	40593632	ID=Msa0500300-mRNA-1:cds;Parent=Msa0500300-mRNA-1
chr3_4	CDS	40593094	40593253	40593094	ID=Msa0500300-mRNA-1:cds;Parent=Msa0500300-mRNA-1
chr3_4	CDS	40592681	40592833	40592681	ID=Msa0500300-mRNA-1:cds;Parent=Msa0500300-mRNA-1
chr3_4	CDS	40592355	40592555	40592355	ID=Msa0500300-mRNA-1:cds;Parent=Msa0500300-mRNA-1
chr3_4	CDS	40592183	40592271	40592183	ID=Msa0500300-mRNA-1:cds;Parent=Msa0500300-mRNA-1
chr3_4	three_prime_UTR	40591914	40592182	40591914	ID=Msa0500300-mRNA-1:three_prime_utr;Parent=Msa0500300-mRNA-1
chr3_4	three_prime_UTR	40591739	40591890	40591739	ID=Msa0500300-mRNA-1:three_prime_utr;Parent=Msa0500300-mRNA-1
chr3_4	three_prime_UTR	40589832	40589865	40589832	ID=Msa0500300-mRNA-1:three_prime_utr;Parent=Msa0500300-mRNA-1

Gene Sequence

>Msa0500300

ATGGTGATTTTCGCAGAGGAGCGGTTGGACTCAGATCGTGCCTTAACAGAGATGTTTCATAGTTTCCGTTCTGAGTTTGTGCCTAGTAGAGAAGAGAGAATAATGGATGATTCCACTGATCGGTTTCCCATCGGAATGCGTGTTCTCGCTGTCGACGACGATCCTCCTTCTCTCTTGCTTTTGGAAACTCTCCTCCGATCATGTCAATACCACGTAACCACAACTAGTAAAGCAATAACAGCATTGACTATGTTGCGAGAAAACATAGACATGTTTGACCTGGTAATCGCTGAAGTTCACATGCCTGACATGGATGGACTCAAGCTGCTGGAACTTGTAGGACTTGAAATGGATCTTCCTGTCATAACGTTGTCTGTATATAGTGATACGGAGCTGGTGATGAAGGCAATTTCCCATGGTGCCCGTGATTTTCTGCTGAAACCTGTGAGATTGGAGGAACTAAGGAACATTTGGCAGCATGTAATTCGGAATAAGAAGTCCCAGTTTGTTTGGTCTGTGGAGCTGCACCGCAAGTTTCTTGAAACTGTTAATCAGTTAGGAGTTGACAAGGCTGTACCCAAAAAGATTTTTGGTTTGATGAATGTTGAAAATATTACAAGGGAAGACGTGGCGACCCATCTCCAGGCACTTTTCTAG

Protein sequence

>Msa0500300

MVIFAEERLDSDRALTEMFHSFRSEFVPSREERIMDDSTDRFPIGMRVLAVDDDPPSLLLLETLLRSCQYHVTTTSKAITALTMLRENIDMFDLVIAEVHMPDMDGLKLLELVGLEMDLPVITLSVYSDTELVMKAISHGARDFLLKPVRLEELRNIWQHVIRNKKSQFVWSVELHRKFLETVNQLGVDKAVPKKIFGLMNVENITREDVATHLQALF*