AlfalfaGEDB | Msa0501740

M. sativa cultivar zhongmu-4 / Msa0501740

KEGG: K12606
GO: -
PFAM: PF04078.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0501740	XP_013459842.1	96.838	253	6	1	21	271	2	254	7.89e-172	487

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0501740	sp\|Q5PQL2\|CNOT9_RAT	75.664	226	55	0	44	269	24	249	2.35e-122	354

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0501740	A0A072UXT2	96.838	253	6	1	21	271	2	254	3.77e-172	487

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TFs/TRs:

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Gene ID	Type	Classification
Msa0501740	TR	Rcd1-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0501740	MtrunA17_Chr3g0099561	96.838	253	6	1	21	271	2	254	7.25e-176	487
Msa0501740	MtrunA17_Chr1g0177151	62.992	254	92	1	9	262	2	253	7.56e-108	313
Msa0501740	MtrunA17_Chr8g0392781	56.794	287	87	2	16	265	8	294	4.16e-99	294
Msa0501740	MtrunA17_Chr7g0224601	64.602	226	76	2	43	264	28	253	4.33e-93	277
Msa0501740	MtrunA17_Chr6g0477171	65.534	206	71	0	64	269	45	250	4.07e-90	268
Msa0501740	MtrunA17_Chr5g0444621	54.464	224	102	0	48	271	25	248	3.96e-76	233
Msa0501740	MtrunA17_Chr3g0083821	57.377	122	44	2	62	179	13	130	1.62e-38	131
Msa0501740	MtrunA17_Chr3g0083811	55.405	74	33	0	191	264	1	74	5.18e-19	80.5
Msa0501740	MtrunA17_Chr5g0444631	66.038	53	18	0	117	169	4	56	7.51e-18	75.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0501740	AT3G20800.1	83.774	265	40	1	10	271	3	267	2.19e-155	436
Msa0501740	AT5G12980.1	82.278	237	42	0	35	271	27	263	1.91e-143	405
Msa0501740	AT2G32550.2	34.632	231	141	5	44	264	51	281	4.29e-36	131
Msa0501740	AT2G32550.3	35.407	209	127	4	64	264	34	242	1.66e-34	126
Msa0501740	AT2G32550.1	35.294	187	113	4	86	264	1	187	2.94e-29	111

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Find 77 sgRNAs with CRISPR-Local

Find 458 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCATCTAACCTGGAGC+AGG	0.510559	3_4:+43258732	None:intergenic
AAAGATTCTGTTGGACGATG+TGG	0.506606	3_4:-43255966	Msa0501740:CDS
AAATGAATTCCATAGCAATG+GGG	0.741455	3_4:+43259731	None:intergenic
AACAACAACTCTTCTTCATT+CGG	0.406492	3_4:-43262400	Msa0501740:CDS
AACTCATCTAACCTGGAGCA+GGG	0.624665	3_4:+43258733	None:intergenic
AACTGATTCACAATGTTGGA+GGG	0.575243	3_4:-43254896	Msa0501740:exon
AAGATCTAGCCCCATTGCTA+TGG	0.486924	3_4:-43259740	Msa0501740:CDS
AAGATTCTGTTGGACGATGT+GGG	0.563510	3_4:-43255965	Msa0501740:CDS
AATCAGTACACTCACCGTTT+TGG	0.342035	3_4:+43256725	None:intergenic
AATCGGGTTCCTGCACTGCA+AGG	0.603945	3_4:-43254873	Msa0501740:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAACAACTCTTCTTCATT+CGG	-	chr3_4:43254726-43254745	Msa0501740:intron	30.0%
GAAAAAGGTGAAAGAGAAAA+TGG	+	chr3_4:43254891-43254910	None:intergenic	30.0%
TAAATACGTGATTAGGGTTA+TGG	-	chr3_4:43254953-43254972	Msa0501740:intron	30.0%
TTGTTATGTCATGTGTTGAA+AGG	-	chr3_4:43255177-43255196	Msa0501740:intron	30.0%
ATGTCATGTCATATAATGTC+CGG	+	chr3_4:43255309-43255328	None:intergenic	30.0%
TATGTGTTGAAACTTTGTTC+TGG	-	chr3_4:43255449-43255468	Msa0501740:intron	30.0%
CACCACATTTACGAAATTTA+CGG	-	chr3_4:43256060-43256079	Msa0501740:intron	30.0%
AACCGTAAATTTCGTAAATG+TGG	+	chr3_4:43256065-43256084	None:intergenic	30.0%
ACAAAACTACGCACAATAAA+GGG	+	chr3_4:43256093-43256112	None:intergenic	30.0%
TACAAAACTACGCACAATAA+AGG	+	chr3_4:43256094-43256113	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	43254669	43262479	43254669	ID=Msa0501740;Name=Msa0501740
chr3_4	mRNA	43254669	43262479	43254669	ID=Msa0501740-mRNA-1;Parent=Msa0501740;Name=Msa0501740-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0\|0.87\|0.88\|1\|0.75\|0.77\|9\|218\|275
chr3_4	exon	43254669	43254718	43254669	ID=Msa0501740-mRNA-1:exon:9171;Parent=Msa0501740-mRNA-1
chr3_4	exon	43254745	43254949	43254745	ID=Msa0501740-mRNA-1:exon:9170;Parent=Msa0501740-mRNA-1
chr3_4	exon	43255818	43256008	43255818	ID=Msa0501740-mRNA-1:exon:9169;Parent=Msa0501740-mRNA-1
chr3_4	exon	43256740	43256835	43256740	ID=Msa0501740-mRNA-1:exon:9168;Parent=Msa0501740-mRNA-1
chr3_4	exon	43257197	43257303	43257197	ID=Msa0501740-mRNA-1:exon:9167;Parent=Msa0501740-mRNA-1
chr3_4	exon	43258223	43258265	43258223	ID=Msa0501740-mRNA-1:exon:9166;Parent=Msa0501740-mRNA-1
chr3_4	exon	43258745	43258834	43258745	ID=Msa0501740-mRNA-1:exon:9165;Parent=Msa0501740-mRNA-1
chr3_4	exon	43259707	43259778	43259707	ID=Msa0501740-mRNA-1:exon:9164;Parent=Msa0501740-mRNA-1
chr3_4	exon	43262288	43262479	43262288	ID=Msa0501740-mRNA-1:exon:9163;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43262288	43262479	43262288	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43259707	43259778	43259707	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43258745	43258834	43258745	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43258223	43258265	43258223	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43257197	43257303	43257197	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43256740	43256835	43256740	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43255818	43256008	43255818	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	CDS	43254913	43254949	43254913	ID=Msa0501740-mRNA-1:cds;Parent=Msa0501740-mRNA-1
chr3_4	three_prime_UTR	43254745	43254912	43254745	ID=Msa0501740-mRNA-1:three_prime_utr;Parent=Msa0501740-mRNA-1
chr3_4	three_prime_UTR	43254669	43254718	43254669	ID=Msa0501740-mRNA-1:three_prime_utr;Parent=Msa0501740-mRNA-1

Gene Sequence

>Msa0501740

ATGTCTACATCACTAGCCACATGTGAAAATTGCCCCCAATCCGTGTCTTCTTCTATGAACAACAACTCTTCTTCATTCGGTTCTATTCCTCCTTCTCCCGCTTCTTCCAACTCGATGGCTTCTTCTGAACAACTCGTTCTCGAACTCAGCAATCCCGAACTCCGTGAAAACGCTCTTCTCGAACTCTCTAAGAAGAGGGAGCAATTTCAAGATCTAGCCCCATTGCTATGGAATTCATTTGGTACTATTGCAGCACTTCTGCAGGAAATTGTTTCAATATACCCTGTTTTGTCTCCTCCCAACCTCACTCCAGCACAATCAAATCGAGTGTGCAATGCTCTTGCCCTGCTCCAGTGTGTAGCATCTCACCCTGATACAAGGATGCTATTCCTGAATGCGCACATTCCTCTGTATTTGTATCCGTTCCTGAACACAACAAGCAAATCGAGACCTTTTGAGTACTTGAGGCTTACTAGTCTTGGTGTCATCGGTGCATTAGTGAAGGTTGATGATACTGAGGTTATTAGTTTTCTGCTTTCGACTGAAATAATTCCCCTGTGCCTGCGCACTATGGAGATGGGGAGTGAGCTCTCCAAAACGGTTGCAACATTTATTGTTCAAAAGATTCTGTTGGACGATGTGGGCTTGGACTATATTTGTACTACAGCTGAGCGCTTTTTCGCTGTTGGTCGAGTTCTTGGAAACATGGTGGCAGCTCTTGCTGAGCAACCATCATCTCGTCTGTTGAAGCATATCATTCGATGCTATCTTCGTCTGTCAGATAACCCGAGGAGGACCCAACAACTAGGAAGTGGCTGCAACAACTGA

Protein sequence

>Msa0501740

MSTSLATCENCPQSVSSSMNNNSSSFGSIPPSPASSNSMASSEQLVLELSNPELRENALLELSKKREQFQDLAPLLWNSFGTIAALLQEIVSIYPVLSPPNLTPAQSNRVCNALALLQCVASHPDTRMLFLNAHIPLYLYPFLNTTSKSRPFEYLRLTSLGVIGALVKVDDTEVISFLLSTEIIPLCLRTMEMGSELSKTVATFIVQKILLDDVGLDYICTTAERFFAVGRVLGNMVAALAEQPSSRLLKHIIRCYLRLSDNPRRTQQLGSGCNN*