Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0502630 | XP_003600264.2 | 92.000 | 225 | 12 | 2 | 1 | 225 | 1 | 219 | 8.48e-148 | 432 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0502630 | sp|Q9SJ41|C3H18_ARATH | 30.603 | 232 | 141 | 8 | 1 | 225 | 1 | 219 | 8.82e-22 | 96.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0502630 | G7J214 | 92.000 | 225 | 12 | 2 | 1 | 225 | 1 | 219 | 4.05e-148 | 432 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0502630 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0502630 | MtrunA17_Chr3g0101151 | 92.000 | 225 | 12 | 2 | 1 | 225 | 1 | 219 | 7.80e-152 | 432 |
| Msa0502630 | MtrunA17_Chr5g0445571 | 48.387 | 248 | 98 | 8 | 1 | 225 | 1 | 241 | 3.66e-61 | 200 |
| Msa0502630 | MtrunA17_Chr3g0090551 | 27.711 | 249 | 119 | 7 | 1 | 188 | 1 | 249 | 1.14e-18 | 84.3 |
| Msa0502630 | MtrunA17_Chr4g0020571 | 28.111 | 217 | 118 | 7 | 1 | 188 | 1 | 208 | 3.44e-14 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0502630 | AT3G52980.1 | 29.237 | 236 | 127 | 6 | 1 | 225 | 1 | 207 | 1.61e-23 | 97.4 |
| Msa0502630 | AT2G05160.3 | 30.603 | 232 | 141 | 8 | 1 | 225 | 1 | 219 | 7.67e-23 | 96.3 |
| Msa0502630 | AT2G05160.2 | 30.603 | 232 | 141 | 8 | 1 | 225 | 1 | 219 | 8.97e-23 | 96.3 |
| Msa0502630 | AT2G05160.1 | 30.603 | 232 | 141 | 8 | 1 | 225 | 1 | 219 | 8.97e-23 | 96.3 |
| Msa0502630 | AT3G51950.1 | 33.163 | 196 | 99 | 7 | 1 | 188 | 1 | 172 | 3.55e-20 | 89.0 |
| Msa0502630 | AT3G51950.3 | 33.163 | 196 | 99 | 7 | 1 | 188 | 1 | 172 | 3.55e-20 | 89.0 |
| Msa0502630 | AT3G51950.2 | 33.163 | 196 | 99 | 7 | 1 | 188 | 1 | 172 | 3.55e-20 | 89.0 |
| Msa0502630 | AT3G51950.4 | 33.163 | 196 | 99 | 7 | 1 | 188 | 1 | 172 | 3.55e-20 | 89.0 |
| Msa0502630 | AT3G63450.7 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 6.83e-14 | 70.5 |
| Msa0502630 | AT3G63450.3 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 7.12e-14 | 70.5 |
| Msa0502630 | AT3G63450.4 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 7.12e-14 | 70.5 |
| Msa0502630 | AT3G63450.5 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 7.12e-14 | 70.5 |
| Msa0502630 | AT3G63450.2 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 7.15e-14 | 70.5 |
| Msa0502630 | AT3G63450.1 | 52.381 | 63 | 30 | 0 | 1 | 63 | 1 | 63 | 7.15e-14 | 70.5 |
| Msa0502630 | AT3G21100.2 | 33.136 | 169 | 86 | 5 | 1 | 146 | 1 | 165 | 4.54e-13 | 68.2 |
| Msa0502630 | AT3G21100.1 | 33.136 | 169 | 86 | 5 | 1 | 146 | 1 | 165 | 4.54e-13 | 68.2 |
| Msa0502630 | AT3G21100.4 | 33.136 | 169 | 86 | 5 | 1 | 146 | 1 | 165 | 4.68e-13 | 68.2 |
| Msa0502630 | AT3G21100.3 | 33.136 | 169 | 86 | 5 | 1 | 146 | 1 | 165 | 4.68e-13 | 68.2 |
Find 37 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTGCCGGCGAAGAATTTC+CGG | 0.247410 | 3_4:+45148524 | None:intergenic |
| TGCTAAATTAGCTTCGTTTC+CGG | 0.258908 | 3_4:-45148622 | Msa0502630:CDS |
| GTGCCTATTGTCATAAATTC+TGG | 0.297480 | 3_4:+45148453 | None:intergenic |
| ATGATCTTTGTAGCATGTTC+TGG | 0.302836 | 3_4:+45148684 | None:intergenic |
| ACCATGATGCAGATTGTAAT+AGG | 0.338016 | 3_4:+45148485 | None:intergenic |
| GATCCGTGAAGTTGCCCTTA+AGG | 0.396350 | 3_4:-45148592 | Msa0502630:CDS |
| ACAATGCAGTGCGGGTTTGA+TGG | 0.410144 | 3_4:+45148424 | None:intergenic |
| GACATAGTCTCTCATATGTA+TGG | 0.411648 | 3_4:-45148097 | Msa0502630:CDS |
| ATTTCAGCAAAGGATATTGC+AGG | 0.419277 | 3_4:-45148165 | Msa0502630:CDS |
| GCGCAACTAGAGTCGGAAAT+CGG | 0.439780 | 3_4:-45148025 | Msa0502630:CDS |
| AGTCCAGAATTTATGACAAT+AGG | 0.443283 | 3_4:-45148456 | Msa0502630:CDS |
| GCAGGCATGGAAATAGTTGT+AGG | 0.444946 | 3_4:-45148147 | Msa0502630:CDS |
| TCTAGTTGCGCCAGTGATCC+AGG | 0.446585 | 3_4:+45148037 | None:intergenic |
| CCATGATGCAGATTGTAATA+GGG | 0.449338 | 3_4:+45148486 | None:intergenic |
| GAACATGCTACAAAGATCAT+TGG | 0.453611 | 3_4:-45148681 | Msa0502630:CDS |
| GAGTGAACCTGAAAACTTGC+CGG | 0.457349 | 3_4:+45148510 | None:intergenic |
| AACGATGTCACCTGGATCAC+TGG | 0.458759 | 3_4:-45148047 | Msa0502630:CDS |
| CAAACCCGCACTGCATTGTT+CGG | 0.459652 | 3_4:-45148420 | Msa0502630:CDS |
| TGGCCTTAAGGGCAACTTCA+CGG | 0.473570 | 3_4:+45148589 | None:intergenic |
| CAAAACTGAGCTTCAAAAGA+TGG | 0.480853 | 3_4:-45148568 | Msa0502630:CDS |
| GCTCGCCGCTATCTTGCATA+AGG | 0.491407 | 3_4:+45148652 | None:intergenic |
| AGCAAAGGATATTGCAGGCA+TGG | 0.511519 | 3_4:-45148160 | Msa0502630:CDS |
| CCCTATTACAATCTGCATCA+TGG | 0.512386 | 3_4:-45148486 | Msa0502630:CDS |
| CCGCACTGCATTGTTCGGTT+CGG | 0.517240 | 3_4:-45148415 | Msa0502630:CDS |
| CGAACCGAACAATGCAGTGC+GGG | 0.526153 | 3_4:+45148416 | None:intergenic |
| GTAGCTGTAGCCAATTTGAA+TGG | 0.531666 | 3_4:-45148244 | Msa0502630:intron |
| AGTTGTAGGTTCTATCATCA+TGG | 0.533176 | 3_4:-45148133 | Msa0502630:CDS |
| ACTTCACGGATCAAGTTATC+CGG | 0.533559 | 3_4:+45148603 | None:intergenic |
| TATCTACATAGTTCAAAAGC+AGG | 0.534771 | 3_4:-45148321 | Msa0502630:intron |
| TGGATATTTCTGAATGTACA+AGG | 0.547604 | 3_4:-45148737 | Msa0502630:CDS |
| ACGAGATCGATAATCTCGTC+CGG | 0.562404 | 3_4:-45148543 | Msa0502630:CDS |
| ATCACTGGCGCAACTAGAGT+CGG | 0.573776 | 3_4:-45148032 | Msa0502630:CDS |
| CTCGTCCGGAAATTCTTCGC+CGG | 0.593097 | 3_4:-45148529 | Msa0502630:CDS |
| ACATGTCACTATTTCAGCAA+AGG | 0.593665 | 3_4:-45148175 | Msa0502630:CDS |
| GATGAACAAACGATGTCACC+TGG | 0.614780 | 3_4:-45148055 | Msa0502630:CDS |
| TATCTCCTTATGCAAGATAG+CGG | 0.677531 | 3_4:-45148657 | Msa0502630:CDS |
| CCGAACCGAACAATGCAGTG+CGG | 0.731305 | 3_4:+45148415 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATGTATGGAAAAATTGAA+TGG | - | chr3_4:45148487-45148506 | Msa0502630:CDS | 20.0% |
| ! | ATGAACACAAATATCTACAT+TGG | - | chr3_4:45148458-45148477 | Msa0502630:CDS | 25.0% |
| !! | ACCAATTTTTCCATTCAAAT+TGG | + | chr3_4:45148522-45148541 | None:intergenic | 25.0% |
| ! | TGAATGGAAAAATTGGTAAA+AGG | - | chr3_4:45148525-45148544 | Msa0502630:CDS | 25.0% |
| !!! | AAATAGTGACATGTTTTGAA+TGG | + | chr3_4:45148572-45148591 | None:intergenic | 25.0% |
| ! | TGGATATTTCTGAATGTACA+AGG | - | chr3_4:45148016-45148035 | Msa0502630:CDS | 30.0% |
| AGTCCAGAATTTATGACAAT+AGG | - | chr3_4:45148297-45148316 | Msa0502630:intron | 30.0% | |
| TATCTACATAGTTCAAAAGC+AGG | - | chr3_4:45148432-45148451 | Msa0502630:CDS | 30.0% | |
| GCCAATTTGAATGGAAAAAT+TGG | - | chr3_4:45148518-45148537 | Msa0502630:CDS | 30.0% | |
| ATGATCTTTGTAGCATGTTC+TGG | + | chr3_4:45148072-45148091 | None:intergenic | 35.0% | |
| GAACATGCTACAAAGATCAT+TGG | - | chr3_4:45148072-45148091 | Msa0502630:CDS | 35.0% | |
| TATCTCCTTATGCAAGATAG+CGG | - | chr3_4:45148096-45148115 | Msa0502630:CDS | 35.0% | |
| ! | TGCTAAATTAGCTTCGTTTC+CGG | - | chr3_4:45148131-45148150 | Msa0502630:CDS | 35.0% |
| !!! | CATCTTTTGAAGCTCAGTTT+TGG | + | chr3_4:45148187-45148206 | None:intergenic | 35.0% |
| CAAAACTGAGCTTCAAAAGA+TGG | - | chr3_4:45148185-45148204 | Msa0502630:CDS | 35.0% | |
| CCATGATGCAGATTGTAATA+GGG | + | chr3_4:45148270-45148289 | None:intergenic | 35.0% | |
| ACCATGATGCAGATTGTAAT+AGG | + | chr3_4:45148271-45148290 | None:intergenic | 35.0% | |
| GTGCCTATTGTCATAAATTC+TGG | + | chr3_4:45148303-45148322 | None:intergenic | 35.0% | |
| ! | ACATTGGTCTCTCATATGTA+TGG | - | chr3_4:45148474-45148493 | Msa0502630:CDS | 35.0% |
| ACATGTCACTATTTCAGCAA+AGG | - | chr3_4:45148578-45148597 | Msa0502630:CDS | 35.0% | |
| ATTTCAGCAAAGGATATTGC+AGG | - | chr3_4:45148588-45148607 | Msa0502630:CDS | 35.0% | |
| AGTTGTAGGTTCTATCATCA+TGG | - | chr3_4:45148620-45148639 | Msa0502630:CDS | 35.0% | |
| GACATAGTCTCTCATATGTA+TGG | - | chr3_4:45148656-45148675 | Msa0502630:CDS | 35.0% | |
| ACTTCACGGATCAAGTTATC+CGG | + | chr3_4:45148153-45148172 | None:intergenic | 40.0% | |
| !!! | AAGCTCAGTTTTGGCCTTAA+GGG | + | chr3_4:45148178-45148197 | None:intergenic | 40.0% |
| CCCTATTACAATCTGCATCA+TGG | - | chr3_4:45148267-45148286 | Msa0502630:intron | 40.0% | |
| GTAGCTGTAGCCAATTTGAA+TGG | - | chr3_4:45148509-45148528 | Msa0502630:CDS | 40.0% | |
| !!! | GAAGCTCAGTTTTGGCCTTA+AGG | + | chr3_4:45148179-45148198 | None:intergenic | 45.0% |
| ACGAGATCGATAATCTCGTC+CGG | - | chr3_4:45148210-45148229 | Msa0502630:CDS | 45.0% | |
| GAGTGAACCTGAAAACTTGC+CGG | + | chr3_4:45148246-45148265 | None:intergenic | 45.0% | |
| AGCAAAGGATATTGCAGGCA+TGG | - | chr3_4:45148593-45148612 | Msa0502630:CDS | 45.0% | |
| GCAGGCATGGAAATAGTTGT+AGG | - | chr3_4:45148606-45148625 | Msa0502630:CDS | 45.0% | |
| GATGAACAAACGATGTCACC+TGG | - | chr3_4:45148698-45148717 | Msa0502630:CDS | 45.0% | |
| GATCCGTGAAGTTGCCCTTA+AGG | - | chr3_4:45148161-45148180 | Msa0502630:CDS | 50.0% | |
| ! | TGGCCTTAAGGGCAACTTCA+CGG | + | chr3_4:45148167-45148186 | None:intergenic | 50.0% |
| ACTTGCCGGCGAAGAATTTC+CGG | + | chr3_4:45148232-45148251 | None:intergenic | 50.0% | |
| ! | TTCTTCGCCGGCAAGTTTTC+AGG | - | chr3_4:45148236-45148255 | Msa0502630:CDS | 50.0% |
| ACAATGCAGTGCGGGTTTGA+TGG | + | chr3_4:45148332-45148351 | None:intergenic | 50.0% | |
| CAAACCCGCACTGCATTGTT+CGG | - | chr3_4:45148333-45148352 | Msa0502630:CDS | 50.0% | |
| AACGATGTCACCTGGATCAC+TGG | - | chr3_4:45148706-45148725 | Msa0502630:CDS | 50.0% | |
| ATCACTGGCGCAACTAGAGT+CGG | - | chr3_4:45148721-45148740 | Msa0502630:CDS | 50.0% | |
| GCGCAACTAGAGTCGGAAAT+CGG | - | chr3_4:45148728-45148747 | Msa0502630:CDS | 50.0% | |
| GCTCGCCGCTATCTTGCATA+AGG | + | chr3_4:45148104-45148123 | None:intergenic | 55.0% | |
| CTCGTCCGGAAATTCTTCGC+CGG | - | chr3_4:45148224-45148243 | Msa0502630:CDS | 55.0% | |
| CGAACCGAACAATGCAGTGC+GGG | + | chr3_4:45148340-45148359 | None:intergenic | 55.0% | |
| CCGAACCGAACAATGCAGTG+CGG | + | chr3_4:45148341-45148360 | None:intergenic | 55.0% | |
| CCGCACTGCATTGTTCGGTT+CGG | - | chr3_4:45148338-45148357 | Msa0502630:CDS | 55.0% | |
| TCTAGTTGCGCCAGTGATCC+AGG | + | chr3_4:45148719-45148738 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 45148015 | 45148760 | 45148015 | ID=Msa0502630;Name=Msa0502630 |
| chr3_4 | mRNA | 45148015 | 45148760 | 45148015 | ID=Msa0502630-mRNA-1;Parent=Msa0502630;Name=Msa0502630-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|228 |
| chr3_4 | exon | 45148322 | 45148760 | 45148322 | ID=Msa0502630-mRNA-1:exon:9609;Parent=Msa0502630-mRNA-1 |
| chr3_4 | exon | 45148015 | 45148262 | 45148015 | ID=Msa0502630-mRNA-1:exon:9608;Parent=Msa0502630-mRNA-1 |
| chr3_4 | CDS | 45148322 | 45148760 | 45148322 | ID=Msa0502630-mRNA-1:cds;Parent=Msa0502630-mRNA-1 |
| chr3_4 | CDS | 45148015 | 45148262 | 45148015 | ID=Msa0502630-mRNA-1:cds;Parent=Msa0502630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
