AlfalfaGEDB | Msa0503720

M. sativa cultivar zhongmu-4 / Msa0503720

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006950 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009909 GO:0010048 GO:0031974 GO:0031981 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043565 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048580 GO:0048831 GO:0050789 GO:0050793 GO:0050896 GO:0051239 GO:0065007 GO:0070013 GO:0097159 GO:1901363 GO:2000026 GO:2000241
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0503720	XP_003600455.1	84.507	284	40	2	1	283	1	281	1.10e-170	483

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0503720	sp\|Q8L3W1\|VRN1_ARATH	26.686	341	179	11	8	283	1	335	5.76e-29	115

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0503720	G8A2Z2	84.507	284	40	2	1	283	1	281	5.25e-171	483

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TFs/TRs:

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Gene ID	Type	Classification
Msa0503720	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0503720	MtrunA17_Chr3g0103181	75.618	283	65	2	1	283	1	279	5.69e-151	423
Msa0503720	MtrunA17_Chr3g0103191	80.952	252	45	3	1	250	1	251	5.19e-141	397
Msa0503720	MtrunA17_Chr3g0103201	86.364	220	29	1	65	283	1	220	2.47e-136	384
Msa0503720	MtrunA17_Chr3g0103171	68.966	261	79	1	25	283	5	265	3.61e-125	357
Msa0503720	MtrunA17_Chr3g0103151	77.640	161	36	0	1	161	1	161	1.68e-88	260
Msa0503720	MtrunA17_Chr3g0103161	69.421	121	37	0	162	282	2	122	3.25e-53	169
Msa0503720	MtrunA17_Chr1g0163071	35.714	294	144	10	26	283	2	286	6.29e-46	156
Msa0503720	MtrunA17_Chr8g0390841	33.657	309	155	8	10	283	37	330	3.37e-45	159
Msa0503720	MtrunA17_Chr8g0390841	49.231	65	32	1	219	283	454	517	4.19e-11	63.2
Msa0503720	MtrunA17_Chr1g0208771	33.106	293	147	7	25	283	12	289	2.36e-41	148

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0503720	AT3G18990.1	26.686	341	179	11	8	283	1	335	5.86e-30	115
Msa0503720	AT3G18990.2	26.686	341	179	11	8	283	1	335	1.49e-29	115
Msa0503720	AT1G49475.1	40.336	119	67	3	11	127	33	149	1.25e-18	82.0
Msa0503720	AT4G01580.1	31.884	138	86	2	11	146	29	160	2.12e-17	78.6
Msa0503720	AT3G18960.2	38.000	100	60	1	11	108	29	128	9.22e-16	74.3
Msa0503720	AT3G18960.1	38.000	100	60	1	11	108	29	128	9.28e-16	74.7
Msa0503720	AT3G18960.3	40.476	84	50	0	25	108	8	91	4.48e-13	66.6
Msa0503720	AT4G33280.1	23.333	300	200	9	10	283	23	318	8.20e-13	68.2
Msa0503720	AT3G53310.1	33.077	130	74	4	1	118	1	129	7.35e-12	64.7
Msa0503720	AT2G16210.1	27.660	282	179	9	12	280	19	288	7.86e-12	64.7

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Find 56 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTCGAACTTTCTTATG+TGG	0.519561	3_4:+47268535	None:intergenic
AAAGAACTTCATCAATGAAT+TGG	0.387908	3_4:-47268398	Msa0503720:CDS
AAATACCAAATGAGTTTACT+AGG	0.476109	3_4:-47268915	Msa0503720:CDS
AAATATGTGTACATCAAACC+TGG	0.553885	3_4:+47268714	None:intergenic
AAGAATGTGATGCTGCTGAT+TGG	0.482287	3_4:-47268143	Msa0503720:CDS
AATCTATTTCCACTGCATTC+CGG	0.411258	3_4:+47268689	None:intergenic
AATGAGTTTACTAGGAGACA+TGG	0.629813	3_4:-47268907	Msa0503720:CDS
AATTGGCCGTCGCCTTTCCG+CGG	0.479288	3_4:-47268075	Msa0503720:CDS
ACCAGCCCGCGGAAAGGCGA+CGG	0.528367	3_4:+47268069	None:intergenic
ACTCTTTCATCCATGGCCAG+AGG	0.664430	3_4:-47269247	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCACTAAATGGAAAGTTTAT+TGG	-	chr3_4:47268369-47268388	Msa0503720:CDS	30.0%
AAAAAATCGATGGTGAGATT+TGG	-	chr3_4:47268393-47268412	Msa0503720:CDS	30.0%
TTGAATATAAAGGAACTTCC+AGG	-	chr3_4:47268480-47268499	Msa0503720:intron	30.0%
AAATATGTGTACATCAAACC+TGG	+	chr3_4:47268501-47268520	None:intergenic	30.0%
TTGATGTACACATATTTGAC+CGG	-	chr3_4:47268504-47268523	Msa0503720:intron	30.0%
TAAAACAGAGAAGAAACTCT+TGG	+	chr3_4:47268753-47268772	None:intergenic	30.0%
GTATGTTCTACCAGATTAAT+TGG	+	chr3_4:47268915-47268934	None:intergenic	30.0%
TACTTATTTGCAAGAGAAAG+TGG	-	chr3_4:47269165-47269184	Msa0503720:intron	30.0%
GCACTAAATGGAAAGTTTAT+TGG	-	chr3_4:47268369-47268388	Msa0503720:CDS	30.0%
AAAAAATCGATGGTGAGATT+TGG	-	chr3_4:47268393-47268412	Msa0503720:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	47267971	47269263	47267971	ID=Msa0503720;Name=Msa0503720
chr3_4	mRNA	47267971	47269263	47267971	ID=Msa0503720-mRNA-1;Parent=Msa0503720;Name=Msa0503720-mRNA-1;_AED=0.06;_eAED=0.06;_QI=6\|0.33\|0.25\|1\|1\|1\|4\|0\|283
chr3_4	exon	47267971	47268204	47267971	ID=Msa0503720-mRNA-1:exon:10156;Parent=Msa0503720-mRNA-1
chr3_4	exon	47268296	47268441	47268296	ID=Msa0503720-mRNA-1:exon:10155;Parent=Msa0503720-mRNA-1
chr3_4	exon	47268539	47268938	47268539	ID=Msa0503720-mRNA-1:exon:10154;Parent=Msa0503720-mRNA-1
chr3_4	exon	47269186	47269263	47269186	ID=Msa0503720-mRNA-1:exon:10153;Parent=Msa0503720-mRNA-1
chr3_4	five_prime_UTR	47269258	47269263	47269258	ID=Msa0503720-mRNA-1:five_prime_utr;Parent=Msa0503720-mRNA-1
chr3_4	CDS	47269186	47269257	47269186	ID=Msa0503720-mRNA-1:cds;Parent=Msa0503720-mRNA-1
chr3_4	CDS	47268539	47268938	47268539	ID=Msa0503720-mRNA-1:cds;Parent=Msa0503720-mRNA-1
chr3_4	CDS	47268296	47268441	47268296	ID=Msa0503720-mRNA-1:cds;Parent=Msa0503720-mRNA-1
chr3_4	CDS	47267971	47268204	47267971	ID=Msa0503720-mRNA-1:cds;Parent=Msa0503720-mRNA-1

Gene Sequence

>Msa0503720

ATGGCCAGAGGAAGTTCTATCCTTCCCATCCGTTTCTTCAAAATCATTCTCCAAACTAATCTTCAAAAGATTAAAATACCAAATGAGTTTACTAGGAGACATGGAGTTCATCTATCAAATCCAGTGTTGATAAAGTCTCTTGATGGCACTAAATGGAAAGTTTATTGGAAAAAAATCGATGGTGAGATTTGGTTTGAAAAGGGTTGGAAAACTTTTACTGAAAATTACTCTCTTGGACATGGCTCTTTGGTGGTGTTTGAATATAAAGGAACTTCCAGGTTTGATGTACACATATTTGACCGGAATGCAGTGGAAATAGATTATGATTCTTCATGTGACACTGATGATGAAAAAGACAATGTTGATGATAGTGATGATGAATCAGTGGAGATTTTGGATGAATGGCTTAATCGGAAGAAGGCTAGACAAAGATCACCTTCCGTTTCTCCTCGACCACATAAGAAAGTTCGAGGTGATCATGAGAAGACTACTAAAAGAACTTCATCAATGAATTGGCCAAAGGATGCTAGAGCTCAGGAAGTGGCTCAAAATTTCATCTCATGCAATCATTTTTTCACAATTCTCATCAAGCCAATTAATCTGGTAGAACATACTCTGTCTGTACCGGATTTTAAAGGGGTTATTGAGAACAAGGAGAAGAATGTGATGCTGCTGATTGGGAAAATATCATGGAATGTGAAACTACTTCGTTCTTATGAGAGCAAAATTGGCCGTCGCCTTTCCGCGGGCTGGTACTTATTTGCAAGAGAAAGTGGACTGAAATCAGGAGATGTTTGTGTCTTTGAACTGATAAACAAGAAAGATTTGGTTTTTAAGGTTCATGTTTTCTAA

Protein sequence

>Msa0503720

MARGSSILPIRFFKIILQTNLQKIKIPNEFTRRHGVHLSNPVLIKSLDGTKWKVYWKKIDGEIWFEKGWKTFTENYSLGHGSLVVFEYKGTSRFDVHIFDRNAVEIDYDSSCDTDDEKDNVDDSDDESVEILDEWLNRKKARQRSPSVSPRPHKKVRGDHEKTTKRTSSMNWPKDARAQEVAQNFISCNHFFTILIKPINLVEHTLSVPDFKGVIENKEKNVMLLIGKISWNVKLLRSYESKIGRRLSAGWYLFARESGLKSGDVCVFELINKKDLVFKVHVF*