AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0504930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504930 MtrunA17_Chr3g0105711 88.095 546 59 5 1 544 1 542 0.0 922
Msa0504930 MtrunA17_Chr3g0136081 49.217 575 196 13 7 543 2 518 9.18e-165 478
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0504930 AT5G16100.2 25.253 297 178 6 267 528 153 440 1.14e-23 104

Find 110 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAAGTCCATGGTAATTT+TGG 0.212819 3_4:-50114245 None:intergenic
GGGTCCATTATTGCTATTAA+TGG 0.250205 3_4:-50114220 None:intergenic
ATTGCTATTAATGGACAAAT+TGG 0.250376 3_4:-50114211 None:intergenic
CATGGACATGGTAGCAATAT+TGG 0.250557 3_4:+50114432 Msa0504930:CDS
CAAAATAATAATGTTTCATT+TGG 0.253731 3_4:+50114786 Msa0504930:CDS
TTGTCCATTAATAGCAATAA+TGG 0.260366 3_4:+50114216 Msa0504930:CDS
GGTCCTATCGATAATTATTC+AGG 0.280430 3_4:+50115421 Msa0504930:CDS
TGGATTGCGGTTGCTCAAAT+TGG 0.290960 3_4:-50114187 None:intergenic
AGACGAATCATTTACTCTAA+TGG 0.306498 3_4:+50114936 Msa0504930:CDS
ATTGTTGGGCCTCCATTAAC+AGG 0.321028 3_4:-50115121 None:intergenic
TATGAAAATCTTGAGATTCA+TGG 0.321881 3_4:+50115058 Msa0504930:CDS
CAAGAGCAAGTTGGTGAGAT+TGG 0.322818 3_4:+50115982 Msa0504930:CDS
AACGTTGCACCTTCGATACT+TGG 0.325070 3_4:-50114039 None:intergenic
CGCCATGTGACACCTGTTAA+TGG 0.325291 3_4:+50115109 Msa0504930:CDS
TTGGATTGATGATATTAATA+TGG 0.329852 3_4:+50115396 Msa0504930:CDS
CACACTACTGCTGCTGATAA+TGG 0.341115 3_4:-50116620 None:intergenic
CTCTTTGCACCGGATATGAT+TGG 0.357597 3_4:+50115377 Msa0504930:CDS
CTTGAGATTCATGGAAGCTT+GGG 0.357672 3_4:+50115067 Msa0504930:CDS
CATGCTTACAGATGAGATAA+TGG 0.387376 3_4:+50115945 Msa0504930:intron
GATATTAAATTGTAATTGAA+TGG 0.400636 3_4:-50114606 None:intergenic
TGATGAAATAAAGAACTTCC+TGG 0.412875 3_4:+50115282 Msa0504930:CDS
CTATTAATGGACAAATTGGT+TGG 0.419381 3_4:-50114207 None:intergenic
AGCAATACTTGAAAATCAAT+TGG 0.419584 3_4:+50116467 Msa0504930:CDS
TGGAATAAGTAACTTTGAAG+TGG 0.427695 3_4:+50114806 Msa0504930:CDS
CGATTACTTTCATCTACCTA+TGG 0.438638 3_4:+50116243 Msa0504930:CDS
TGTATATATCATTTAGTGTA+GGG 0.456932 3_4:-50114113 None:intergenic
CTTCCTGGATCAGTATTGTA+TGG 0.458075 3_4:+50115297 Msa0504930:CDS
CAATCGTTCGATCAATTGTT+GGG 0.465946 3_4:-50115135 None:intergenic
TCTTGAGATTCATGGAAGCT+TGG 0.468339 3_4:+50115066 Msa0504930:CDS
CACCAAAATTACCATGGACT+TGG 0.468970 3_4:+50114243 Msa0504930:CDS
AGATGTGCACAATTTACATT+GGG 0.476510 3_4:+50116211 Msa0504930:CDS
TCAATCGTTCGATCAATTGT+TGG 0.476535 3_4:-50115136 None:intergenic
ATTCAGTTGTGGCATTGCAT+TGG 0.484885 3_4:-50114472 None:intergenic
CAACAAAATTACCATGGACT+TGG 0.485409 3_4:+50114327 Msa0504930:CDS
GGAAGTTCTTTATTTCATCA+AGG 0.486733 3_4:-50115279 None:intergenic
GGAATAAGTAACTTTGAAGT+GGG 0.489373 3_4:+50114807 Msa0504930:CDS
TCAATTGGACAGGCAAGATC+CGG 0.489751 3_4:+50116482 Msa0504930:CDS
ATCATCAATCCAATCATATC+CGG 0.493113 3_4:-50115386 None:intergenic
TGTATGGAACATAATTCAGC+AGG 0.494565 3_4:+50115313 Msa0504930:CDS
TTGATCAAGTGCCAACGCTT+CGG 0.497530 3_4:-50114865 None:intergenic
CCATGGCATTCAACATGGAC+GGG 0.499629 3_4:+50114569 Msa0504930:CDS
CCAATGGTTCAAAATCAACT+TGG 0.499953 3_4:+50114759 Msa0504930:CDS
CCATGTCCATGTTGAATACC+AGG 0.503325 3_4:-50114711 None:intergenic
GTAGAACAAAATTAAGAAGA+TGG 0.504860 3_4:+50116320 Msa0504930:CDS
CTAGCCGCTGCTTCTTCCAT+AGG 0.508372 3_4:-50116259 None:intergenic
AGAGTCCTTTCCCTTGTCGC+CGG 0.514274 3_4:-50116501 None:intergenic
ATGTATATATCATTTAGTGT+AGG 0.514577 3_4:-50114114 None:intergenic
AAGATGTGCACAATTTACAT+TGG 0.515263 3_4:+50116210 Msa0504930:CDS
CGATTACAATTTAGTATCGC+CGG 0.515726 3_4:+50114539 Msa0504930:CDS
AAGCGTTGGCACTTGATCAA+TGG 0.517228 3_4:+50114868 Msa0504930:CDS
AAGAAGATGGCCACACAGAA+AGG 0.518680 3_4:+50116333 Msa0504930:CDS
GATTGATGATATTAATATGG+AGG 0.518874 3_4:+50115399 Msa0504930:CDS
TACTTGAAAATCAATTGGAC+AGG 0.519249 3_4:+50116472 Msa0504930:CDS
ACCATGGCATTCAACATGGA+CGG 0.528445 3_4:+50114568 Msa0504930:CDS
GTTCCATACAATACTGATCC+AGG 0.531567 3_4:-50115300 None:intergenic
CACCAGAGTGTGGTTGAACA+TGG 0.539370 3_4:+50114414 Msa0504930:CDS
GCGTTCAACACGGACGGAGT+AGG 0.542691 3_4:+50114502 Msa0504930:CDS
ATGTCACAACAAAATGATCC+TGG 0.543235 3_4:+50114693 Msa0504930:CDS
GCGACATAATTCAACGCAGT+TGG 0.549096 3_4:-50116579 None:intergenic
TCATGGTATTCAACAAGGGC+AGG 0.551808 3_4:+50114641 Msa0504930:CDS
AAGATCCGGCGACAAGGGAA+AGG 0.552910 3_4:+50116496 Msa0504930:CDS
ATATCACCAGAAAATGATCA+TGG 0.553458 3_4:+50114624 Msa0504930:CDS
CTCCATTAACAGGTGTCACA+TGG 0.555997 3_4:-50115111 None:intergenic
CCCGTCCATGTTGAATGCCA+TGG 0.556371 3_4:-50114569 None:intergenic
GACTTGGAATACTTCAACGA+GGG 0.562322 3_4:+50114259 Msa0504930:CDS
CAGGCAAGATCCGGCGACAA+GGG 0.569973 3_4:+50116491 Msa0504930:CDS
TACATGAATAACAATAACAA+TGG 0.572167 3_4:+50114132 Msa0504930:CDS
AATGACCATGGCATTCAACA+TGG 0.573215 3_4:+50114564 Msa0504930:CDS
ATTGATGATATTAATATGGA+GGG 0.585774 3_4:+50115400 Msa0504930:CDS
GGTATTCAACAAGGGCAGGA+TGG 0.589069 3_4:+50114645 Msa0504930:CDS
GATACTAAATTGTAATCGAA+TGG 0.589440 3_4:-50114534 None:intergenic
TCCGTCCGTGTTGAACGCCA+TGG 0.589945 3_4:-50114497 None:intergenic
GTCCATGTTCAACCACACTC+TGG 0.590094 3_4:-50114416 None:intergenic
GAGCAAGAGCAAGAGCAAGT+TGG 0.592584 3_4:+50115973 Msa0504930:CDS
TTGAGATTCATGGAAGCTTG+GGG 0.597747 3_4:+50115068 Msa0504930:CDS
AATGATCATGGTATTCAACA+AGG 0.598505 3_4:+50114636 Msa0504930:CDS
GTAGAAGTATTCCAAGTCCA+TGG 0.598550 3_4:-50114338 None:intergenic
CCTGGTATTCAACATGGACA+TGG 0.598984 3_4:+50114711 Msa0504930:CDS
GTTGAAGTATTCCAAGTCCA+TGG 0.599108 3_4:-50114254 None:intergenic
ACAGGCAAGATCCGGCGACA+AGG 0.599603 3_4:+50116490 Msa0504930:CDS
ACGCCATGGTCATTCAGTTG+TGG 0.599712 3_4:-50114483 None:intergenic
AAACCTGAATAATTATCGAT+AGG 0.600017 3_4:-50115424 None:intergenic
TCAACATCAGACCGAAGCGT+TGG 0.600663 3_4:+50114854 Msa0504930:CDS
TATTATGAGACTCTTTGCAC+CGG 0.606478 3_4:+50115367 Msa0504930:CDS
GGCATTCAACATGGACGGGG+TGG 0.611696 3_4:+50114573 Msa0504930:CDS
CTATCCCAACAAAATTACCA+TGG 0.618972 3_4:+50114321 Msa0504930:CDS
ACCATGGCGTTCAACACGGA+CGG 0.619182 3_4:+50114496 Msa0504930:CDS
GGACTTGGAATACTTCTACA+AGG 0.619567 3_4:+50114342 Msa0504930:CDS
AATGATCCTGGTATTCAACA+TGG 0.622879 3_4:+50114705 Msa0504930:CDS
CAACAACAAAATTCTTGCAG+TGG 0.625689 3_4:+50114900 Msa0504930:CDS
CATGGCATTCAACATGGACG+GGG 0.625970 3_4:+50114570 Msa0504930:CDS
AATGACCATGGCGTTCAACA+CGG 0.633314 3_4:+50114492 Msa0504930:CDS
CAAGACATGATTGATGATTG+TGG 0.634370 3_4:+50116546 Msa0504930:CDS
CATGTGACACCTGTTAATGG+AGG 0.638273 3_4:+50115112 Msa0504930:CDS
TGGACAAATTGGTTGGATTG+CGG 0.639366 3_4:-50114200 None:intergenic
TCTACCTATGGAAGAAGCAG+CGG 0.639381 3_4:+50116255 Msa0504930:CDS
TGCTGCTGATAATGGTGCGG+CGG 0.640455 3_4:-50116612 None:intergenic
GGACCCCACCAAAATTACCA+TGG 0.646800 3_4:+50114237 Msa0504930:CDS
GGAATACTTCTACAAGGACA+TGG 0.662894 3_4:+50114348 Msa0504930:CDS
TACTGCTGCTGATAATGGTG+CGG 0.670583 3_4:-50116615 None:intergenic
ATGATCATGGTATTCAACAA+GGG 0.672665 3_4:+50114637 Msa0504930:CDS
GTATGGAACATAATTCAGCA+GGG 0.676056 3_4:+50115314 Msa0504930:CDS
ATAAAATTACCTGCTCTGAG+AGG 0.686791 3_4:-50116023 None:intergenic
GACAAGGGAAAGGACTCTAG+AGG 0.686791 3_4:+50116506 Msa0504930:CDS
CCAACAAAATCACCAGAGTG+TGG 0.691943 3_4:+50114404 Msa0504930:CDS
GTATCGCCGGAAAATGACCA+TGG 0.708673 3_4:+50114552 Msa0504930:CDS
ATGCCACAACTGAATGACCA+TGG 0.716852 3_4:+50114480 Msa0504930:CDS
AGTGTGGTTGAACATGGACA+TGG 0.753622 3_4:+50114420 Msa0504930:CDS
GACATGATTGATGATTGTGG+CGG 0.760229 3_4:+50116549 Msa0504930:CDS
GGACTTGGAATACTTCAACG+AGG 0.774898 3_4:+50114258 Msa0504930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTCATATTGAAAAAATTT+GGG - chr3_4:50114092-50114111 None:intergenic 15.0%
!! TTACTCATATTGAAAAAATT+TGG - chr3_4:50114093-50114112 None:intergenic 15.0%
!! GATATTAAATTGTAATTGAA+TGG - chr3_4:50114609-50114628 None:intergenic 15.0%
!! CAAAATAATAATGTTTCATT+TGG + chr3_4:50114786-50114805 Msa0504930:CDS 15.0%
!! CATATATATATCAAAATAAC+TGG - chr3_4:50115009-50115028 None:intergenic 15.0%
!!! GTTTATTTTGATTCTTATAT+AGG + chr3_4:50115028-50115047 Msa0504930:intron 15.0%
!!! CTTTTAGAAATATATTTGTT+TGG + chr3_4:50115576-50115595 Msa0504930:intron 15.0%
!!! TATCATAATGATTTTGATTT+GGG + chr3_4:50115813-50115832 Msa0504930:intron 15.0%
!!! TTTTTTGTGTGTATTATTTT+TGG + chr3_4:50116365-50116384 Msa0504930:intron 15.0%
!! TGTATATATCATTTAGTGTA+GGG - chr3_4:50114116-50114135 None:intergenic 20.0%
!! ATGTATATATCATTTAGTGT+AGG - chr3_4:50114117-50114136 None:intergenic 20.0%
!! TACATGAATAACAATAACAA+TGG + chr3_4:50114132-50114151 Msa0504930:CDS 20.0%
!!! TTTCTTGTAGTGATTAATTT+TGG - chr3_4:50114985-50115004 None:intergenic 20.0%
!!! TTGGATTGATGATATTAATA+TGG + chr3_4:50115396-50115415 Msa0504930:CDS 20.0%
!!! ATTGATGATATTAATATGGA+GGG + chr3_4:50115400-50115419 Msa0504930:CDS 20.0%
!!! CTTTTAGAAATACATTTGTT+TGG + chr3_4:50115669-50115688 Msa0504930:intron 20.0%
!!! GTATCATAATGATTTTGATT+TGG + chr3_4:50115812-50115831 Msa0504930:intron 20.0%
!!! ATCATAATGATTTTGATTTG+GGG + chr3_4:50115814-50115833 Msa0504930:intron 20.0%
!!! TTTTTAATGTTATTACGTTG+AGG + chr3_4:50115905-50115924 Msa0504930:intron 20.0%
!! ATATTGTATGTATTGAATAG+AGG + chr3_4:50116185-50116204 Msa0504930:intron 20.0%
!! TGTGTCTTAAAATTAAGTAT+AGG + chr3_4:50116422-50116441 Msa0504930:intron 20.0%
! ATTGAAAAAATTTGGGTCAA+TGG - chr3_4:50114085-50114104 None:intergenic 25.0%
!! ATTGGAATGATGATTTTGTT+GGG - chr3_4:50114172-50114191 None:intergenic 25.0%
!! AATTGGAATGATGATTTTGT+TGG - chr3_4:50114173-50114192 None:intergenic 25.0%
!! ATTGCTATTAATGGACAAAT+TGG - chr3_4:50114214-50114233 None:intergenic 25.0%
! TTGTCCATTAATAGCAATAA+TGG + chr3_4:50114216-50114235 Msa0504930:CDS 25.0%
! GATACTAAATTGTAATCGAA+TGG - chr3_4:50114537-50114556 None:intergenic 25.0%
! TATGAAAATCTTGAGATTCA+TGG + chr3_4:50115058-50115077 Msa0504930:CDS 25.0%
! TGTAATATTACACTAAGACT+TGG - chr3_4:50115169-50115188 None:intergenic 25.0%
!! GATTGATGATATTAATATGG+AGG + chr3_4:50115399-50115418 Msa0504930:CDS 25.0%
! AAACCTGAATAATTATCGAT+AGG - chr3_4:50115427-50115446 None:intergenic 25.0%
! GTAGAACAAAATTAAGAAGA+TGG + chr3_4:50116320-50116339 Msa0504930:CDS 25.0%
! CACACATTAAATTAGCAAAA+TGG - chr3_4:50116406-50116425 None:intergenic 25.0%
! AGCAATACTTGAAAATCAAT+TGG + chr3_4:50116467-50116486 Msa0504930:CDS 25.0%
!! AAAAACAATCCAAGTATCGA+AGG + chr3_4:50114030-50114049 Msa0504930:CDS 30.0%
! TTTTGTTGGGACAAATTTGT+GGG - chr3_4:50114159-50114178 None:intergenic 30.0%
! ATTTTGTTGGGACAAATTTG+TGG - chr3_4:50114160-50114179 None:intergenic 30.0%
! CTATTAATGGACAAATTGGT+TGG - chr3_4:50114210-50114229 None:intergenic 30.0%
! AAGTCCATGGTAATTTTGTT+GGG - chr3_4:50114328-50114347 None:intergenic 30.0%
ATATCACCAGAAAATGATCA+TGG + chr3_4:50114624-50114643 Msa0504930:CDS 30.0%
! TGAATACCATGATCATTTTC+TGG - chr3_4:50114633-50114652 None:intergenic 30.0%
AATGATCATGGTATTCAACA+AGG + chr3_4:50114636-50114655 Msa0504930:CDS 30.0%
ATGATCATGGTATTCAACAA+GGG + chr3_4:50114637-50114656 Msa0504930:CDS 30.0%
!! ATTTTGTTGTGACATTGCAT+GGG - chr3_4:50114688-50114707 None:intergenic 30.0%
! TGAAACATTTTCTCTTCCAA+TGG + chr3_4:50114743-50114762 Msa0504930:CDS 30.0%
! TGGAATAAGTAACTTTGAAG+TGG + chr3_4:50114806-50114825 Msa0504930:CDS 30.0%
!! GGAATAAGTAACTTTGAAGT+GGG + chr3_4:50114807-50114826 Msa0504930:CDS 30.0%
AGACGAATCATTTACTCTAA+TGG + chr3_4:50114936-50114955 Msa0504930:CDS 30.0%
!! TGTATCAGTTTTTCTGAGAA+AGG + chr3_4:50115256-50115275 Msa0504930:intron 30.0%
! GGAAGTTCTTTATTTCATCA+AGG - chr3_4:50115282-50115301 None:intergenic 30.0%
TGATGAAATAAAGAACTTCC+TGG + chr3_4:50115282-50115301 Msa0504930:CDS 30.0%
ATCATCAATCCAATCATATC+CGG - chr3_4:50115389-50115408 None:intergenic 30.0%
AAGATGTGCACAATTTACAT+TGG + chr3_4:50116210-50116229 Msa0504930:CDS 30.0%
AGATGTGCACAATTTACATT+GGG + chr3_4:50116211-50116230 Msa0504930:CDS 30.0%
! AATACTTTTACCTTTCTGTG+TGG - chr3_4:50116346-50116365 None:intergenic 30.0%
! TATAGGTGAAATGTCTTTTG+AGG + chr3_4:50116439-50116458 Msa0504930:intron 30.0%
TACTTGAAAATCAATTGGAC+AGG + chr3_4:50116472-50116491 Msa0504930:CDS 30.0%
! GGGTCCATTATTGCTATTAA+TGG - chr3_4:50114223-50114242 None:intergenic 35.0%
!! AAGTCCATGGTAATTTTGGT+GGG - chr3_4:50114244-50114263 None:intergenic 35.0%
! TTCCAAGTCCATGGTAATTT+TGG - chr3_4:50114248-50114267 None:intergenic 35.0%
!!! ATTTTGTTGGGATAGTGGAT+TGG - chr3_4:50114316-50114335 None:intergenic 35.0%
! TGGTAATTTTGTTGGGATAG+TGG - chr3_4:50114321-50114340 None:intergenic 35.0%
CTATCCCAACAAAATTACCA+TGG + chr3_4:50114321-50114340 Msa0504930:CDS 35.0%
! CAAGTCCATGGTAATTTTGT+TGG - chr3_4:50114329-50114348 None:intergenic 35.0%
CAACAAAATTACCATGGACT+TGG + chr3_4:50114327-50114346 Msa0504930:CDS 35.0%
CGATTACAATTTAGTATCGC+CGG + chr3_4:50114539-50114558 Msa0504930:CDS 35.0%
!! CATTTTGTTGTGACATTGCA+TGG - chr3_4:50114689-50114708 None:intergenic 35.0%
ATGTCACAACAAAATGATCC+TGG + chr3_4:50114693-50114712 Msa0504930:CDS 35.0%
AATGATCCTGGTATTCAACA+TGG + chr3_4:50114705-50114724 Msa0504930:CDS 35.0%
!!! CCAAGTTGATTTTGAACCAT+TGG - chr3_4:50114762-50114781 None:intergenic 35.0%
CCAATGGTTCAAAATCAACT+TGG + chr3_4:50114759-50114778 Msa0504930:CDS 35.0%
!!! GAATTTTGTTGTTGTTCGAG+TGG - chr3_4:50114894-50114913 None:intergenic 35.0%
CAACAACAAAATTCTTGCAG+TGG + chr3_4:50114900-50114919 Msa0504930:CDS 35.0%
CAATCGTTCGATCAATTGTT+GGG - chr3_4:50115138-50115157 None:intergenic 35.0%
TCAATCGTTCGATCAATTGT+TGG - chr3_4:50115139-50115158 None:intergenic 35.0%
TGTATGGAACATAATTCAGC+AGG + chr3_4:50115313-50115332 Msa0504930:CDS 35.0%
GTATGGAACATAATTCAGCA+GGG + chr3_4:50115314-50115333 Msa0504930:CDS 35.0%
TATTATGAGACTCTTTGCAC+CGG + chr3_4:50115367-50115386 Msa0504930:CDS 35.0%
GGTCCTATCGATAATTATTC+AGG + chr3_4:50115421-50115440 Msa0504930:CDS 35.0%
GTTTGTCTTCGAAATCATGA+AGG + chr3_4:50115443-50115462 Msa0504930:intron 35.0%
CATGCTTACAGATGAGATAA+TGG + chr3_4:50115945-50115964 Msa0504930:intron 35.0%
AGATTGGAACACCTGAAAAA+AGG + chr3_4:50115998-50116017 Msa0504930:CDS 35.0%
! ATAAAATTACCTGCTCTGAG+AGG - chr3_4:50116026-50116045 None:intergenic 35.0%
TTGAACAATATATACTCGCG+AGG + chr3_4:50116060-50116079 Msa0504930:intron 35.0%
CGATTACTTTCATCTACCTA+TGG + chr3_4:50116243-50116262 Msa0504930:CDS 35.0%
! CAAGACATGATTGATGATTG+TGG + chr3_4:50116546-50116565 Msa0504930:CDS 35.0%
TGGACAAATTGGTTGGATTG+CGG - chr3_4:50114203-50114222 None:intergenic 40.0%
!! AGTCCATGGTAATTTTGGTG+GGG - chr3_4:50114243-50114262 None:intergenic 40.0%
! CAAGTCCATGGTAATTTTGG+TGG - chr3_4:50114245-50114264 None:intergenic 40.0%
CACCAAAATTACCATGGACT+TGG + chr3_4:50114243-50114262 Msa0504930:CDS 40.0%
! GTTGAAGTATTCCAAGTCCA+TGG - chr3_4:50114257-50114276 None:intergenic 40.0%
GACTTGGAATACTTCAACGA+GGG + chr3_4:50114259-50114278 Msa0504930:CDS 40.0%
!! GTAGAAGTATTCCAAGTCCA+TGG - chr3_4:50114341-50114360 None:intergenic 40.0%
GGACTTGGAATACTTCTACA+AGG + chr3_4:50114342-50114361 Msa0504930:CDS 40.0%
GGAATACTTCTACAAGGACA+TGG + chr3_4:50114348-50114367 Msa0504930:CDS 40.0%
! CACACTCTGGTGATTTTGTT+GGG - chr3_4:50114406-50114425 None:intergenic 40.0%
CATGGACATGGTAGCAATAT+TGG + chr3_4:50114432-50114451 Msa0504930:CDS 40.0%
ATTCAGTTGTGGCATTGCAT+TGG - chr3_4:50114475-50114494 None:intergenic 40.0%
!! TGAATGCCATGGTCATTTTC+CGG - chr3_4:50114561-50114580 None:intergenic 40.0%
AATGACCATGGCATTCAACA+TGG + chr3_4:50114564-50114583 Msa0504930:CDS 40.0%
TCTTGAGATTCATGGAAGCT+TGG + chr3_4:50115066-50115085 Msa0504930:CDS 40.0%
CTTGAGATTCATGGAAGCTT+GGG + chr3_4:50115067-50115086 Msa0504930:CDS 40.0%
TTGAGATTCATGGAAGCTTG+GGG + chr3_4:50115068-50115087 Msa0504930:CDS 40.0%
CTTCCTGGATCAGTATTGTA+TGG + chr3_4:50115297-50115316 Msa0504930:CDS 40.0%
GTTCCATACAATACTGATCC+AGG - chr3_4:50115303-50115322 None:intergenic 40.0%
!!! TCTGAGAGGTTCCTTTTTTC+AGG - chr3_4:50116012-50116031 None:intergenic 40.0%
! GACATGATTGATGATTGTGG+CGG + chr3_4:50116549-50116568 Msa0504930:CDS 40.0%
AACGTTGCACCTTCGATACT+TGG - chr3_4:50114042-50114061 None:intergenic 45.0%
TGGATTGCGGTTGCTCAAAT+TGG - chr3_4:50114190-50114209 None:intergenic 45.0%
GGACTTGGAATACTTCAACG+AGG + chr3_4:50114258-50114277 Msa0504930:CDS 45.0%
! CCACACTCTGGTGATTTTGT+TGG - chr3_4:50114407-50114426 None:intergenic 45.0%
CCAACAAAATCACCAGAGTG+TGG + chr3_4:50114404-50114423 Msa0504930:CDS 45.0%
AGTGTGGTTGAACATGGACA+TGG + chr3_4:50114420-50114439 Msa0504930:CDS 45.0%
ATGCCACAACTGAATGACCA+TGG + chr3_4:50114480-50114499 Msa0504930:CDS 45.0%
AATGACCATGGCGTTCAACA+CGG + chr3_4:50114492-50114511 Msa0504930:CDS 45.0%
ACCATGGCATTCAACATGGA+CGG + chr3_4:50114568-50114587 Msa0504930:CDS 45.0%
TCATGGTATTCAACAAGGGC+AGG + chr3_4:50114641-50114660 Msa0504930:CDS 45.0%
CCATGTCCATGTTGAATACC+AGG - chr3_4:50114714-50114733 None:intergenic 45.0%
CCTGGTATTCAACATGGACA+TGG + chr3_4:50114711-50114730 Msa0504930:CDS 45.0%
TTGATCAAGTGCCAACGCTT+CGG - chr3_4:50114868-50114887 None:intergenic 45.0%
!! AAGCGTTGGCACTTGATCAA+TGG + chr3_4:50114868-50114887 Msa0504930:CDS 45.0%
! CTCCATTAACAGGTGTCACA+TGG - chr3_4:50115114-50115133 None:intergenic 45.0%
CATGTGACACCTGTTAATGG+AGG + chr3_4:50115112-50115131 Msa0504930:CDS 45.0%
ATTGTTGGGCCTCCATTAAC+AGG - chr3_4:50115124-50115143 None:intergenic 45.0%
CTCTTTGCACCGGATATGAT+TGG + chr3_4:50115377-50115396 Msa0504930:CDS 45.0%
GGGTTGTGACTGAGTATTTC+TGG + chr3_4:50115834-50115853 Msa0504930:intron 45.0%
!! CAAGAGCAAGTTGGTGAGAT+TGG + chr3_4:50115982-50116001 Msa0504930:CDS 45.0%
AAAAAGGAACCTCTCAGAGC+AGG + chr3_4:50116014-50116033 Msa0504930:CDS 45.0%
TCTACCTATGGAAGAAGCAG+CGG + chr3_4:50116255-50116274 Msa0504930:CDS 45.0%
AAGAAGATGGCCACACAGAA+AGG + chr3_4:50116333-50116352 Msa0504930:CDS 45.0%
TCAATTGGACAGGCAAGATC+CGG + chr3_4:50116482-50116501 Msa0504930:CDS 45.0%
GCGACATAATTCAACGCAGT+TGG - chr3_4:50116582-50116601 None:intergenic 45.0%
!! TACTGCTGCTGATAATGGTG+CGG - chr3_4:50116618-50116637 None:intergenic 45.0%
GGACCCCACCAAAATTACCA+TGG + chr3_4:50114237-50114256 Msa0504930:CDS 50.0%
CACCAGAGTGTGGTTGAACA+TGG + chr3_4:50114414-50114433 Msa0504930:CDS 50.0%
GTCCATGTTCAACCACACTC+TGG - chr3_4:50114419-50114438 None:intergenic 50.0%
ACGCCATGGTCATTCAGTTG+TGG - chr3_4:50114486-50114505 None:intergenic 50.0%
GTATCGCCGGAAAATGACCA+TGG + chr3_4:50114552-50114571 Msa0504930:CDS 50.0%
CCATGGCATTCAACATGGAC+GGG + chr3_4:50114569-50114588 Msa0504930:CDS 50.0%
CATGGCATTCAACATGGACG+GGG + chr3_4:50114570-50114589 Msa0504930:CDS 50.0%
GGTATTCAACAAGGGCAGGA+TGG + chr3_4:50114645-50114664 Msa0504930:CDS 50.0%
TCAACATCAGACCGAAGCGT+TGG + chr3_4:50114854-50114873 Msa0504930:CDS 50.0%
CGCCATGTGACACCTGTTAA+TGG + chr3_4:50115109-50115128 Msa0504930:CDS 50.0%
GAGCAAGAGCAAGAGCAAGT+TGG + chr3_4:50115973-50115992 Msa0504930:CDS 50.0%
AAGCGACAGACTTGTCTGCA+TGG + chr3_4:50116123-50116142 Msa0504930:intron 50.0%
GACAAGGGAAAGGACTCTAG+AGG + chr3_4:50116506-50116525 Msa0504930:CDS 50.0%
ACCATGGCGTTCAACACGGA+CGG + chr3_4:50114496-50114515 Msa0504930:CDS 55.0%
CCCGTCCATGTTGAATGCCA+TGG - chr3_4:50114572-50114591 None:intergenic 55.0%
CTAGCCGCTGCTTCTTCCAT+AGG - chr3_4:50116262-50116281 None:intergenic 55.0%
AAGATCCGGCGACAAGGGAA+AGG + chr3_4:50116496-50116515 Msa0504930:CDS 55.0%
AGAGTCCTTTCCCTTGTCGC+CGG - chr3_4:50116504-50116523 None:intergenic 55.0%
!! TGCTGCTGATAATGGTGCGG+CGG - chr3_4:50116615-50116634 None:intergenic 55.0%
TCCGTCCGTGTTGAACGCCA+TGG - chr3_4:50114500-50114519 None:intergenic 60.0%
GCGTTCAACACGGACGGAGT+AGG + chr3_4:50114502-50114521 Msa0504930:CDS 60.0%
GGCATTCAACATGGACGGGG+TGG + chr3_4:50114573-50114592 Msa0504930:CDS 60.0%
ACAGGCAAGATCCGGCGACA+AGG + chr3_4:50116490-50116509 Msa0504930:CDS 60.0%
CAGGCAAGATCCGGCGACAA+GGG + chr3_4:50116491-50116510 Msa0504930:CDS 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 50114018 50116638 50114018 ID=Msa0504930;Name=Msa0504930
chr3_4 mRNA 50114018 50116638 50114018 ID=Msa0504930-mRNA-1;Parent=Msa0504930;Name=Msa0504930-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0|0|0|1|1|1|6|0|550
chr3_4 exon 50114018 50114957 50114018 ID=Msa0504930-mRNA-1:exon:10808;Parent=Msa0504930-mRNA-1
chr3_4 exon 50115050 50115158 50115050 ID=Msa0504930-mRNA-1:exon:10809;Parent=Msa0504930-mRNA-1
chr3_4 exon 50115264 50115442 50115264 ID=Msa0504930-mRNA-1:exon:10810;Parent=Msa0504930-mRNA-1
chr3_4 exon 50115956 50116035 50115956 ID=Msa0504930-mRNA-1:exon:10811;Parent=Msa0504930-mRNA-1
chr3_4 exon 50116205 50116354 50116205 ID=Msa0504930-mRNA-1:exon:10812;Parent=Msa0504930-mRNA-1
chr3_4 exon 50116444 50116638 50116444 ID=Msa0504930-mRNA-1:exon:10813;Parent=Msa0504930-mRNA-1
chr3_4 CDS 50114018 50114957 50114018 ID=Msa0504930-mRNA-1:cds;Parent=Msa0504930-mRNA-1
chr3_4 CDS 50115050 50115158 50115050 ID=Msa0504930-mRNA-1:cds;Parent=Msa0504930-mRNA-1
chr3_4 CDS 50115264 50115442 50115264 ID=Msa0504930-mRNA-1:cds;Parent=Msa0504930-mRNA-1
chr3_4 CDS 50115956 50116035 50115956 ID=Msa0504930-mRNA-1:cds;Parent=Msa0504930-mRNA-1
chr3_4 CDS 50116205 50116354 50116205 ID=Msa0504930-mRNA-1:cds;Parent=Msa0504930-mRNA-1
chr3_4 CDS 50116444 50116638 50116444 ID=Msa0504930-mRNA-1:cds;Parent=Msa0504930-mRNA-1
Gene Sequence

>Msa0504930

ATGGCAAACAGAAAAAACAATCCAAGTATCGAAGGTGCAACGTTTAAATTTAATGATGATGATGCCATTGACCCAAATTTTTTCAATATGAGTAACCCTACACTAAATGATATATACATGAATAACAATAACAATGGACCCACAAATTTGTCCCAACAAAATCATCATTCCAATTTGAGCAACCGCAATCCAACCAATTTGTCCATTAATAGCAATAATGGACCCCACCAAAATTACCATGGACTTGGAATACTTCAACGAGGGCAACAAAGTAGTAATTTCAATAGTCACTTTTCCAATCCACTATCCCAACAAAATTACCATGGACTTGGAATACTTCTACAAGGACATGGTAGTAGTAATGAACAATTTCATACTACACTATCCCAACAAAATCACCAGAGTGTGGTTGAACATGGACATGGTAGCAATATTGGAAATGTCTCATTTAATTCCAATGCAATGCCACAACTGAATGACCATGGCGTTCAACACGGACGGAGTAGGAATTACGATCCATTCGATTACAATTTAGTATCGCCGGAAAATGACCATGGCATTCAACATGGACGGGGTGGTAATTATGATCCATTCAATTACAATTTAATATCACCAGAAAATGATCATGGTATTCAACAAGGGCAGGATGGTAATAATGATGTATTTTCCCATGCAATGTCACAACAAAATGATCCTGGTATTCAACATGGACATGGTAGTACAAATGAAACATTTTCTCTTCCAATGGTTCAAAATCAACTTGGTCAGCAAAATAATAATGTTTCATTTGGAATAAGTAACTTTGAAGTGGGTAGTTCGTCACGAATGAATGAGAATCAACATCAGACCGAAGCGTTGGCACTTGATCAATGGCCACTCGAACAACAACAAAATTCTTGCAGTGGTAGTCAAGTTCTTAGACGAATCATTTACTCTAATGGTTTTCAATATGAAAATCTTGAGATTCATGGAAGCTTGGGGTTGATTACACATGCAGTTCGCCATGTGACACCTGTTAATGGAGGCCCAACAATTGATCGAACGATTGATTTTTCTGAGAAAGGCCTTGATGAAATAAAGAACTTCCTGGATCAGTATTGTATGGAACATAATTCAGCAGGGTACTTTCTTATACAAGATTCAATGTGTGCGTATTATGAGACTCTTTGCACCGGATATGATTGGATTGATGATATTAATATGGAGGGTCCTATCGATAATTATTCAGATGAGATAATGGAGCAAGAGCAAGAGCAAGAGCAAGTTGGTGAGATTGGAACACCTGAAAAAAGGAACCTCTCAGAGCAGAGGAAAAGATGTGCACAATTTACATTGGGTGATTTCTGCGATTACTTTCATCTACCTATGGAAGAAGCAGCGGCTAGAGTGAACATAGCTGCATCAACTCTAAAGAAAATTTGTCGTAGAACAAAATTAAGAAGATGGCCACACAGAAAGGTGAAATGTCTTTTGAGGCAAATAGCAATACTTGAAAATCAATTGGACAGGCAAGATCCGGCGACAAGGGAAAGGACTCTAGAGGAAATTAAGCAGCTTAAACAAGACATGATTGATGATTGTGGCGGACATACACCAACTGCGTTGAATTATGTCGCTAACTTCTTGCCGCCGCACCATTATCAGCAGCAGTAG
Protein sequence

>Msa0504930

MANRKNNPSIEGATFKFNDDDAIDPNFFNMSNPTLNDIYMNNNNNGPTNLSQQNHHSNLSNRNPTNLSINSNNGPHQNYHGLGILQRGQQSSNFNSHFSNPLSQQNYHGLGILLQGHGSSNEQFHTTLSQQNHQSVVEHGHGSNIGNVSFNSNAMPQLNDHGVQHGRSRNYDPFDYNLVSPENDHGIQHGRGGNYDPFNYNLISPENDHGIQQGQDGNNDVFSHAMSQQNDPGIQHGHGSTNETFSLPMVQNQLGQQNNNVSFGISNFEVGSSSRMNENQHQTEALALDQWPLEQQQNSCSGSQVLRRIIYSNGFQYENLEIHGSLGLITHAVRHVTPVNGGPTIDRTIDFSEKGLDEIKNFLDQYCMEHNSAGYFLIQDSMCAYYETLCTGYDWIDDINMEGPIDNYSDEIMEQEQEQEQVGEIGTPEKRNLSEQRKRCAQFTLGDFCDYFHLPMEEAAARVNIAASTLKKICRRTKLRRWPHRKVKCLLRQIAILENQLDRQDPATRERTLEEIKQLKQDMIDDCGGHTPTALNYVANFLPPHHYQQQ*