AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0508670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0508670 MtrunA17_Chr3g0113121 92.593 216 16 0 30 245 1 216 1.00e-141 395
Msa0508670 MtrunA17_Chr5g0436501 41.401 157 78 6 50 200 48 196 9.41e-21 88.2
Msa0508670 MtrunA17_Chr5g0429521 37.209 129 68 2 78 200 80 201 2.28e-17 79.7
Msa0508670 MtrunA17_Chr5g0440731 57.143 77 27 4 121 196 102 173 3.42e-17 79.0
Msa0508670 MtrunA17_Chr3g0104071 33.516 182 98 4 19 200 58 216 5.62e-17 78.6
Msa0508670 MtrunA17_Chr5g0409841 36.567 134 75 3 67 196 32 159 1.54e-16 76.3
Msa0508670 MtrunA17_Chr5g0440741 58.904 73 24 4 125 196 101 168 5.37e-16 75.5
Msa0508670 MtrunA17_Chr7g0241761 31.443 194 92 8 26 200 38 209 5.63e-16 75.9
Msa0508670 MtrunA17_Chr5g0409831 43.023 86 43 2 90 169 37 122 9.51e-16 73.9
Msa0508670 MtrunA17_Chr3g0135671 38.462 117 56 2 79 195 58 158 7.33e-15 71.6
Msa0508670 MtrunA17_Chr1g0210471 29.240 171 94 4 29 199 49 192 9.41e-14 68.6
Msa0508670 MtrunA17_Chr4g0059701 33.333 117 66 2 53 169 25 129 1.55e-13 68.6
Msa0508670 MtrunA17_Chr1g0162081 45.570 79 36 2 121 199 119 190 1.36e-12 65.9
Msa0508670 MtrunA17_Chr6g0488471 37.692 130 57 6 112 241 87 192 1.43e-12 65.9
Msa0508670 MtrunA17_Chr2g0331031 39.759 83 43 1 115 197 100 175 1.62e-12 66.2
Msa0508670 MtrunA17_Chr4g0055931 36.607 112 52 2 88 199 71 163 4.28e-12 64.7
Msa0508670 MtrunA17_Chr4g0062741 44.304 79 37 2 121 199 135 206 5.80e-12 64.3
Msa0508670 MtrunA17_Chr5g0415191 55.102 49 22 0 121 169 99 147 6.49e-12 64.3
Msa0508670 MtrunA17_Chr8g0365011 35.948 153 64 7 61 199 42 174 8.79e-12 63.5
Msa0508670 MtrunA17_Chr8g0389241 44.000 75 34 1 121 195 102 168 1.52e-11 62.8
Msa0508670 MtrunA17_Chr5g0432921 44.792 96 44 5 93 187 85 172 1.79e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0508670 AT5G05790.2 41.406 128 63 3 78 201 82 201 7.61e-20 86.3
Msa0508670 AT5G05790.1 41.406 128 63 3 78 201 82 201 7.61e-20 86.3
Msa0508670 AT3G11280.2 35.870 184 90 9 25 201 35 197 5.61e-19 83.6
Msa0508670 AT3G11280.1 35.870 184 90 9 25 201 35 197 5.61e-19 83.6
Msa0508670 AT5G01200.1 45.299 117 50 4 85 200 112 215 3.49e-18 81.6
Msa0508670 AT2G38090.1 37.069 116 63 2 88 200 102 210 2.55e-16 77.0
Msa0508670 AT5G58900.1 36.424 151 72 5 63 200 71 210 4.24e-16 76.3
Msa0508670 AT5G08520.1 36.207 174 84 9 41 198 23 185 1.46e-15 74.7
Msa0508670 AT4G09450.1 39.837 123 61 4 99 215 66 181 4.06e-14 69.3
Msa0508670 AT5G04760.1 49.367 79 33 2 121 199 96 167 1.88e-13 67.8
Msa0508670 AT1G19000.2 50.877 57 28 0 113 169 91 147 6.02e-13 67.4
Msa0508670 AT1G19000.1 50.877 57 28 0 113 169 91 147 6.02e-13 67.4
Msa0508670 AT5G47390.1 35.878 131 66 3 115 245 87 199 9.64e-13 67.4
Msa0508670 AT1G70000.1 41.096 73 41 1 99 169 71 143 1.70e-12 65.9
Msa0508670 AT1G70000.2 41.096 73 41 1 99 169 71 143 1.70e-12 65.9
Msa0508670 AT3G10580.1 46.835 79 33 1 121 199 92 161 3.68e-12 65.1
Msa0508670 AT3G10580.2 46.835 79 33 1 121 199 92 161 3.89e-12 64.7
Msa0508670 AT1G49010.1 45.570 79 36 2 121 199 131 202 4.85e-12 65.1
Msa0508670 AT5G56840.1 52.632 57 27 0 121 177 88 144 2.78e-11 62.0
Msa0508670 AT5G61620.1 45.570 79 36 1 121 199 105 176 5.10e-11 62.0
Msa0508670 AT1G74840.2 55.102 49 22 0 121 169 93 141 6.75e-11 60.8
Msa0508670 AT1G74840.1 55.102 49 22 0 121 169 93 141 7.58e-11 61.2

Find 35 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCTTGATCACATTCT+TGG 0.190525 3_4:-56754963 None:intergenic
TCAACCAATAATCATCTTCT+TGG 0.216049 3_4:-56754296 None:intergenic
ATTTCCAAGAAGATGATTAT+TGG 0.238910 3_4:+56754292 Msa0508670:CDS
GACTCATATGCGTGGTTATT+TGG 0.243758 3_4:+56754880 Msa0508670:CDS
ATAACTACTCTTGATCTTCT+TGG 0.251399 3_4:+56754850 Msa0508670:CDS
AAAGAAATCATTCAAGAAAA+TGG 0.280931 3_4:+56754506 Msa0508670:CDS
AGTTCTATTGTTATTATTAT+CGG 0.281602 3_4:-56754463 None:intergenic
TAGAATGGTAGAGAATATTC+TGG 0.354560 3_4:+56754242 Msa0508670:CDS
TTTGAGCATGGCTTGCTACT+TGG 0.356659 3_4:-56754747 None:intergenic
CATTCAAGAAAATGGTTCAT+TGG 0.366973 3_4:+56754514 Msa0508670:CDS
AAAATATCAAAAGGAAACTT+AGG 0.373972 3_4:+56754812 Msa0508670:CDS
TTGAGCATGGCTTGCTACTT+GGG 0.417939 3_4:-56754746 None:intergenic
TATTTGTAGGCGTTTCTTGA+AGG 0.450413 3_4:+56754654 Msa0508670:intron
TCAAACTCAAATAATAGTTA+TGG 0.460594 3_4:+56754189 Msa0508670:CDS
AAATATCAAAAGGAAACTTA+GGG 0.461487 3_4:+56754813 Msa0508670:CDS
AGATGATTATTGGTTGATGA+AGG 0.467158 3_4:+56754302 Msa0508670:CDS
AAAGTGGAAAGAAATCTCTA+AGG 0.472931 3_4:+56754699 Msa0508670:CDS
ATTTGTAGGCGTTTCTTGAA+GGG 0.494586 3_4:+56754655 Msa0508670:intron
GATCAAGAGTAGTTATATCA+TGG 0.499404 3_4:-56754843 None:intergenic
TACACGGCAAAGACGGAAAG+TGG 0.504210 3_4:+56754683 Msa0508670:CDS
GCATGGCTTGCTACTTGGGA+TGG 0.505418 3_4:-56754742 None:intergenic
TGTTGTCGTCTTCAATCACT+TGG 0.511632 3_4:-56754906 None:intergenic
CATCGAAGAAAGTAGTAGAA+TGG 0.523965 3_4:+56754227 Msa0508670:CDS
AGAATGGTAGAGAATATTCT+GGG 0.545240 3_4:+56754243 Msa0508670:CDS
CAATAGAACTACGAATATTG+TGG 0.554412 3_4:+56754476 Msa0508670:CDS
GGACTTCTACACGGCAAAGA+CGG 0.559413 3_4:+56754676 Msa0508670:CDS
ACTCAGCATTAACAATGGAA+TGG 0.588865 3_4:+56754145 None:intergenic
ATACATATGAACATATCAAG+TGG 0.590413 3_4:-56754998 None:intergenic
TTCTTGAAGGGACTTCTACA+CGG 0.594579 3_4:+56754667 Msa0508670:CDS
GAAGACGACAACAATAACAA+TGG 0.605151 3_4:+56754916 Msa0508670:CDS
TCAGCACAACATCGATGAAG+TGG 0.609150 3_4:+56754356 Msa0508670:CDS
TTATTATTATCGGTTGCATG+AGG 0.632701 3_4:-56754453 None:intergenic
GCACAACATCGATGAAGTGG+TGG 0.668571 3_4:+56754359 Msa0508670:CDS
AATATCAAAAGGAAACTTAG+GGG 0.670754 3_4:+56754814 Msa0508670:CDS
GAAGTGATGACTCATATGCG+TGG 0.764866 3_4:+56754872 Msa0508670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTCTATTGTTATTATTAT+CGG - chr3_4:56754466-56754485 None:intergenic 15.0%
!!! AGTTCTATTGTTATTATTAT+CGG - chr3_4:56754466-56754485 None:intergenic 15.0%
!! TCAAACTCAAATAATAGTTA+TGG + chr3_4:56754189-56754208 Msa0508670:CDS 20.0%
!!! TTTCTTGAATGATTTCTTTT+TGG - chr3_4:56754505-56754524 None:intergenic 20.0%
!! AAAGAAATCATTCAAGAAAA+TGG + chr3_4:56754506-56754525 Msa0508670:CDS 20.0%
!! ATGATAGCAAAAATATCAAA+AGG + chr3_4:56754803-56754822 Msa0508670:CDS 20.0%
!! AAAATATCAAAAGGAAACTT+AGG + chr3_4:56754812-56754831 Msa0508670:CDS 20.0%
!! AAATATCAAAAGGAAACTTA+GGG + chr3_4:56754813-56754832 Msa0508670:CDS 20.0%
!! TCAAACTCAAATAATAGTTA+TGG + chr3_4:56754189-56754208 Msa0508670:CDS 20.0%
!!! TTTCTTGAATGATTTCTTTT+TGG - chr3_4:56754505-56754524 None:intergenic 20.0%
!! AAAGAAATCATTCAAGAAAA+TGG + chr3_4:56754506-56754525 Msa0508670:CDS 20.0%
!! ATGATAGCAAAAATATCAAA+AGG + chr3_4:56754803-56754822 Msa0508670:CDS 20.0%
!! AAAATATCAAAAGGAAACTT+AGG + chr3_4:56754812-56754831 Msa0508670:CDS 20.0%
!! AAATATCAAAAGGAAACTTA+GGG + chr3_4:56754813-56754832 Msa0508670:CDS 20.0%
! ATTTCCAAGAAGATGATTAT+TGG + chr3_4:56754292-56754311 Msa0508670:CDS 25.0%
! GAAACAATAAACGATGAAAA+AGG - chr3_4:56754563-56754582 None:intergenic 25.0%
!!! GACATGGATTTTTTATTTGT+AGG + chr3_4:56754641-56754660 Msa0508670:intron 25.0%
! AATATCAAAAGGAAACTTAG+GGG + chr3_4:56754814-56754833 Msa0508670:CDS 25.0%
! ATTTCCAAGAAGATGATTAT+TGG + chr3_4:56754292-56754311 Msa0508670:CDS 25.0%
! GAAACAATAAACGATGAAAA+AGG - chr3_4:56754563-56754582 None:intergenic 25.0%
!!! GACATGGATTTTTTATTTGT+AGG + chr3_4:56754641-56754660 Msa0508670:intron 25.0%
! AATATCAAAAGGAAACTTAG+GGG + chr3_4:56754814-56754833 Msa0508670:CDS 25.0%
TAGAATGGTAGAGAATATTC+TGG + chr3_4:56754242-56754261 Msa0508670:CDS 30.0%
AGAATGGTAGAGAATATTCT+GGG + chr3_4:56754243-56754262 Msa0508670:CDS 30.0%
TCAACCAATAATCATCTTCT+TGG - chr3_4:56754299-56754318 None:intergenic 30.0%
!! AGATGATTATTGGTTGATGA+AGG + chr3_4:56754302-56754321 Msa0508670:CDS 30.0%
! TTATTATTATCGGTTGCATG+AGG - chr3_4:56754456-56754475 None:intergenic 30.0%
CAATAGAACTACGAATATTG+TGG + chr3_4:56754476-56754495 Msa0508670:CDS 30.0%
CATTCAAGAAAATGGTTCAT+TGG + chr3_4:56754514-56754533 Msa0508670:CDS 30.0%
AAAGTGGAAAGAAATCTCTA+AGG + chr3_4:56754699-56754718 Msa0508670:CDS 30.0%
!!! TTTTTTCGTACTTTTGAGCA+TGG - chr3_4:56754762-56754781 None:intergenic 30.0%
! ATGCTCAAAAGTACGAAAAA+AGG + chr3_4:56754761-56754780 Msa0508670:CDS 30.0%
GATCAAGAGTAGTTATATCA+TGG - chr3_4:56754846-56754865 None:intergenic 30.0%
ATAACTACTCTTGATCTTCT+TGG + chr3_4:56754850-56754869 Msa0508670:CDS 30.0%
TAGAATGGTAGAGAATATTC+TGG + chr3_4:56754242-56754261 Msa0508670:CDS 30.0%
AGAATGGTAGAGAATATTCT+GGG + chr3_4:56754243-56754262 Msa0508670:CDS 30.0%
TCAACCAATAATCATCTTCT+TGG - chr3_4:56754299-56754318 None:intergenic 30.0%
!! AGATGATTATTGGTTGATGA+AGG + chr3_4:56754302-56754321 Msa0508670:CDS 30.0%
! TTATTATTATCGGTTGCATG+AGG - chr3_4:56754456-56754475 None:intergenic 30.0%
CAATAGAACTACGAATATTG+TGG + chr3_4:56754476-56754495 Msa0508670:CDS 30.0%
CATTCAAGAAAATGGTTCAT+TGG + chr3_4:56754514-56754533 Msa0508670:CDS 30.0%
AAAGTGGAAAGAAATCTCTA+AGG + chr3_4:56754699-56754718 Msa0508670:CDS 30.0%
!!! TTTTTTCGTACTTTTGAGCA+TGG - chr3_4:56754762-56754781 None:intergenic 30.0%
! ATGCTCAAAAGTACGAAAAA+AGG + chr3_4:56754761-56754780 Msa0508670:CDS 30.0%
GATCAAGAGTAGTTATATCA+TGG - chr3_4:56754846-56754865 None:intergenic 30.0%
ATAACTACTCTTGATCTTCT+TGG + chr3_4:56754850-56754869 Msa0508670:CDS 30.0%
CATCGAAGAAAGTAGTAGAA+TGG + chr3_4:56754227-56754246 Msa0508670:CDS 35.0%
TATTTGTAGGCGTTTCTTGA+AGG + chr3_4:56754654-56754673 Msa0508670:intron 35.0%
ATTTGTAGGCGTTTCTTGAA+GGG + chr3_4:56754655-56754674 Msa0508670:intron 35.0%
GAAGACGACAACAATAACAA+TGG + chr3_4:56754916-56754935 Msa0508670:CDS 35.0%
CATGATCTTGATCACATTCT+TGG - chr3_4:56754966-56754985 None:intergenic 35.0%
CATCGAAGAAAGTAGTAGAA+TGG + chr3_4:56754227-56754246 Msa0508670:CDS 35.0%
TATTTGTAGGCGTTTCTTGA+AGG + chr3_4:56754654-56754673 Msa0508670:intron 35.0%
ATTTGTAGGCGTTTCTTGAA+GGG + chr3_4:56754655-56754674 Msa0508670:intron 35.0%
GAAGACGACAACAATAACAA+TGG + chr3_4:56754916-56754935 Msa0508670:CDS 35.0%
CATGATCTTGATCACATTCT+TGG - chr3_4:56754966-56754985 None:intergenic 35.0%
TTCTTGAAGGGACTTCTACA+CGG + chr3_4:56754667-56754686 Msa0508670:CDS 40.0%
GACTCATATGCGTGGTTATT+TGG + chr3_4:56754880-56754899 Msa0508670:CDS 40.0%
TGTTGTCGTCTTCAATCACT+TGG - chr3_4:56754909-56754928 None:intergenic 40.0%
TTCTTGAAGGGACTTCTACA+CGG + chr3_4:56754667-56754686 Msa0508670:CDS 40.0%
GACTCATATGCGTGGTTATT+TGG + chr3_4:56754880-56754899 Msa0508670:CDS 40.0%
TGTTGTCGTCTTCAATCACT+TGG - chr3_4:56754909-56754928 None:intergenic 40.0%
TCAGCACAACATCGATGAAG+TGG + chr3_4:56754356-56754375 Msa0508670:CDS 45.0%
!! TCGACGAGCGTTTCACTTTT+TGG + chr3_4:56754399-56754418 Msa0508670:CDS 45.0%
!! TTGAGCATGGCTTGCTACTT+GGG - chr3_4:56754749-56754768 None:intergenic 45.0%
!! TTTGAGCATGGCTTGCTACT+TGG - chr3_4:56754750-56754769 None:intergenic 45.0%
GAAGTGATGACTCATATGCG+TGG + chr3_4:56754872-56754891 Msa0508670:CDS 45.0%
TCAGCACAACATCGATGAAG+TGG + chr3_4:56754356-56754375 Msa0508670:CDS 45.0%
!! TCGACGAGCGTTTCACTTTT+TGG + chr3_4:56754399-56754418 Msa0508670:CDS 45.0%
!! TTGAGCATGGCTTGCTACTT+GGG - chr3_4:56754749-56754768 None:intergenic 45.0%
!! TTTGAGCATGGCTTGCTACT+TGG - chr3_4:56754750-56754769 None:intergenic 45.0%
GAAGTGATGACTCATATGCG+TGG + chr3_4:56754872-56754891 Msa0508670:CDS 45.0%
GCACAACATCGATGAAGTGG+TGG + chr3_4:56754359-56754378 Msa0508670:CDS 50.0%
GCGCACACATGTGTTTGACA+TGG + chr3_4:56754625-56754644 Msa0508670:intron 50.0%
GGACTTCTACACGGCAAAGA+CGG + chr3_4:56754676-56754695 Msa0508670:CDS 50.0%
TACACGGCAAAGACGGAAAG+TGG + chr3_4:56754683-56754702 Msa0508670:CDS 50.0%
GCACAACATCGATGAAGTGG+TGG + chr3_4:56754359-56754378 Msa0508670:CDS 50.0%
GCGCACACATGTGTTTGACA+TGG + chr3_4:56754625-56754644 Msa0508670:intron 50.0%
GGACTTCTACACGGCAAAGA+CGG + chr3_4:56754676-56754695 Msa0508670:CDS 50.0%
TACACGGCAAAGACGGAAAG+TGG + chr3_4:56754683-56754702 Msa0508670:CDS 50.0%
!! GCATGGCTTGCTACTTGGGA+TGG - chr3_4:56754745-56754764 None:intergenic 55.0%
!! GCATGGCTTGCTACTTGGGA+TGG - chr3_4:56754745-56754764 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 56754159 56755005 56754159 ID=Msa0508670;Name=Msa0508670
chr3_4 mRNA 56754159 56755005 56754159 ID=Msa0508670-mRNA-1;Parent=Msa0508670;Name=Msa0508670-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|2|0|245
chr3_4 exon 56754159 56754553 56754159 ID=Msa0508670-mRNA-1:exon:12804;Parent=Msa0508670-mRNA-1
chr3_4 exon 56754663 56755005 56754663 ID=Msa0508670-mRNA-1:exon:12805;Parent=Msa0508670-mRNA-1
chr3_4 CDS 56754159 56754553 56754159 ID=Msa0508670-mRNA-1:cds;Parent=Msa0508670-mRNA-1
chr3_4 CDS 56754663 56755005 56754663 ID=Msa0508670-mRNA-1:cds;Parent=Msa0508670-mRNA-1
Gene Sequence

>Msa0508670

ATGGAATGGATGAATTCTTCTTTGAACTCATCAAACTCAAATAATAGTTATGGCTACATATATGACAGCATCGAAGAAAGTAGTAGAATGGTAGAGAATATTCTGGGTTACAGTCTTCATAGCATGATGAATAATTTCCAAGAAGATGATTATTGGTTGATGAAGGAAACTATGCTATGTAGTGTTGATCAAATCAATCAGCACAACATCGATGAAGTGGTGGTAGAAAATAATATTGCATCGACGAGCGTTTCACTTTTTGGATATGATTCAGTTGATTCTTTCAACTTCTCTCCTCATGCAACCGATAATAATAACAATAGAACTACGAATATTGTGGAATCCAAAAAGAAATCATTCAAGAAAATGGTTCATTGGTCACGTGATGAACACATGCGTTTCTTGAAGGGACTTCTACACGGCAAAGACGGAAAGTGGAAAGAAATCTCTAAGGACTATGTAAAAACAAAGACTCCATCCCAAGTAGCAAGCCATGCTCAAAAGTACGAAAAAAGGCAAAAGCAAAGACTTGATGATGATAGCAAAAATATCAAAAGGAAACTTAGGGGTAGCATCCATGATATAACTACTCTTGATCTTCTTGGAAGTGATGACTCATATGCGTGGTTATTTGGTGACCAAGTGATTGAAGACGACAACAATAACAATGGATCACTCTCAGTCACAACTTTCAACCAAGAATGTGATCAAGATCATGAAGTTGTTGCTTCCACTTGA

Protein sequence

>Msa0508670

MEWMNSSLNSSNSNNSYGYIYDSIEESSRMVENILGYSLHSMMNNFQEDDYWLMKETMLCSVDQINQHNIDEVVVENNIASTSVSLFGYDSVDSFNFSPHATDNNNNRTTNIVESKKKSFKKMVHWSRDEHMRFLKGLLHGKDGKWKEISKDYVKTKTPSQVASHAQKYEKRQKQRLDDDSKNIKRKLRGSIHDITTLDLLGSDDSYAWLFGDQVIEDDNNNNGSLSVTTFNQECDQDHEVVAST*