AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0514090


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0514090 MtrunA17_Chr3g0122071 90.598 117 11 0 213 329 106 222 5.70e-73 225
Msa0514090 MtrunA17_Chr3g0122071 69.744 195 14 2 1 184 1 161 8.46e-54 176
Msa0514090 MtrunA17_Chr4g0064011 54.494 178 57 9 1 174 1 158 2.52e-42 146
Msa0514090 MtrunA17_Chr4g0064011 61.069 131 48 3 203 332 104 232 6.88e-39 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0514090 AT5G22990.1 51.807 83 40 0 220 302 232 314 1.02e-19 88.6
Msa0514090 AT5G22990.1 51.282 78 24 2 136 213 232 295 3.98e-16 78.6
Msa0514090 AT2G18490.1 53.425 73 33 1 207 279 142 213 6.15e-17 79.7
Msa0514090 AT2G18490.1 60.000 60 23 1 123 182 142 200 5.48e-15 73.9
Msa0514090 AT2G18490.2 53.425 73 33 1 207 279 192 263 7.52e-17 80.1
Msa0514090 AT2G18490.2 58.065 62 25 1 123 184 192 252 7.62e-15 74.3
Msa0514090 AT2G15740.1 52.564 78 32 2 209 286 219 291 1.14e-16 80.1
Msa0514090 AT2G15740.1 60.000 60 23 1 125 184 219 277 9.03e-16 77.4
Msa0514090 AT5G42640.1 52.113 71 29 2 213 279 218 287 3.16e-15 75.5
Msa0514090 AT5G42640.1 39.216 102 57 2 85 182 174 274 5.40e-13 68.9

Find 38 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTAATATTTATATTAA+TGG 0.098823 3_4:+64752726 None:intergenic
TCAATTGGTACCAATAATTT+TGG 0.136685 3_4:-64752838 Msa0514090:CDS
AGCATGACTTCCAAAATTAT+TGG 0.299809 3_4:+64752828 None:intergenic
AGCCTACCTCACAAGAAATA+TGG 0.344617 3_4:-64752126 Msa0514090:CDS
AGCCTACCTCACAAGAAATA+TGG 0.344617 3_4:-64752478 Msa0514090:CDS
GAGATTCTTGAAGGTCAGTT+TGG 0.349001 3_4:-64752784 Msa0514090:CDS
ATCGTTCTTGTTCCGATGCA+TGG 0.373109 3_4:+64752200 None:intergenic
ATTTCTTGTGAGGTAGGCTA+TGG 0.375103 3_4:+64752130 None:intergenic
ATTTCTTGTGAGGTAGGCTA+TGG 0.375103 3_4:+64752482 None:intergenic
ACAATAATAGATTGTTTCAA+TGG 0.400008 3_4:-64752894 Msa0514090:CDS
TATTAATATTAATTATTTGA+AGG 0.413280 3_4:+64751814 None:intergenic
AGATTCTTGAAGGTCAGTTT+GGG 0.424623 3_4:-64752783 Msa0514090:CDS
TCGTTCTTGTTCCGATGCAT+GGG 0.447785 3_4:+64752201 None:intergenic
TTATCAGTACAAGCAATAAC+AGG 0.454560 3_4:+64751919 None:intergenic
ACTCATTCATCATCATCAAT+TGG 0.471297 3_4:-64752853 Msa0514090:CDS
AGCAATGAAAATATTGATGA+TGG 0.472887 3_4:-64752159 Msa0514090:CDS
AGCAATGAAAATATTGATGA+TGG 0.472887 3_4:-64752511 Msa0514090:CDS
ACATCTCCAACATCAGCAAC+AGG 0.474279 3_4:+64752565 None:intergenic
AATATTCCTGTTGCTGATGT+TGG 0.486932 3_4:-64752571 Msa0514090:CDS
TCTCATCTTGATCTCCGTGT+AGG 0.496262 3_4:-64752634 Msa0514090:CDS
AAAGCGATGGAGATTCTTGA+AGG 0.521307 3_4:-64752793 Msa0514090:CDS
CTCTTACCCCTCTTCCAAGT+GGG 0.526442 3_4:-64751855 Msa0514090:CDS
TTTCTTGTGAGGTAGGCTAT+GGG 0.526908 3_4:+64752131 None:intergenic
TTTCTTGTGAGGTAGGCTAT+GGG 0.526908 3_4:+64752483 None:intergenic
AGATTGTTTCAATGGATGCA+TGG 0.544477 3_4:-64752886 Msa0514090:CDS
ATGTTAACAAGAACGATATG+AGG 0.552379 3_4:-64752546 Msa0514090:CDS
GGAATATTAATCATTTGAGA+AGG 0.562733 3_4:+64752586 None:intergenic
ATCGGAACAAGAACGATATG+AGG 0.569024 3_4:-64752194 Msa0514090:CDS
TACGGTCCATATTTCTTGTG+AGG 0.577081 3_4:+64752120 None:intergenic
TACGGTCCATATTTCTTGTG+AGG 0.577081 3_4:+64752472 None:intergenic
GCTCTTACCCCTCTTCCAAG+TGG 0.581741 3_4:-64751856 Msa0514090:CDS
GTCCATATTTCTTGTGAGGT+AGG 0.582227 3_4:+64752124 None:intergenic
GTCCATATTTCTTGTGAGGT+AGG 0.582227 3_4:+64752476 None:intergenic
CAGATTCAAAAGCTCAATGA+CGG 0.593887 3_4:-64751958 Msa0514090:CDS
ATTGCTCTTACCCCATGCAT+CGG 0.621144 3_4:-64752212 Msa0514090:CDS
TCCGGGAATCCTAAAAGCGA+TGG 0.625731 3_4:-64752806 Msa0514090:CDS
CGTTCTTGTTCCGATGCATG+GGG 0.640029 3_4:+64752202 None:intergenic
ATTGTGATGATAAGCCTACA+CGG 0.769789 3_4:+64752620 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATAATAGATTGTTTCAA+TGG - chr3_4:64751826-64751845 Msa0514090:CDS 20.0%
!!! TTTTGTTACATTTTGAACAT+GGG + chr3_4:64752626-64752645 None:intergenic 20.0%
!!! ATTTTGTTACATTTTGAACA+TGG + chr3_4:64752627-64752646 None:intergenic 20.0%
!! ACAATAATAGATTGTTTCAA+TGG - chr3_4:64751826-64751845 Msa0514090:CDS 20.0%
!!! TTTTGTTACATTTTGAACAT+GGG + chr3_4:64752626-64752645 None:intergenic 20.0%
!!! ATTTTGTTACATTTTGAACA+TGG + chr3_4:64752627-64752646 None:intergenic 20.0%
!! TCAATTGGTACCAATAATTT+TGG - chr3_4:64751882-64751901 Msa0514090:CDS 25.0%
! GGAATATTAATCATTTGAGA+AGG + chr3_4:64752137-64752156 None:intergenic 25.0%
! AGCAATGAAAATATTGATGA+TGG - chr3_4:64752209-64752228 Msa0514090:CDS 25.0%
!! TTTTGTTGCATTTTGAACAT+GGG + chr3_4:64752274-64752293 None:intergenic 25.0%
!! ATTTTGTTGCATTTTGAACA+TGG + chr3_4:64752275-64752294 None:intergenic 25.0%
! AGAAAAAGAGATACATTTCT+AGG - chr3_4:64752365-64752384 Msa0514090:CDS 25.0%
! AGCAATGAAAATATTGATGA+TGG - chr3_4:64752209-64752228 Msa0514090:CDS 25.0%
! GAAAAAAGAGATACATTTCT+AGG - chr3_4:64752718-64752737 Msa0514090:CDS 25.0%
!! TCAATTGGTACCAATAATTT+TGG - chr3_4:64751882-64751901 Msa0514090:CDS 25.0%
! GGAATATTAATCATTTGAGA+AGG + chr3_4:64752137-64752156 None:intergenic 25.0%
! AGCAATGAAAATATTGATGA+TGG - chr3_4:64752209-64752228 Msa0514090:CDS 25.0%
!! TTTTGTTGCATTTTGAACAT+GGG + chr3_4:64752274-64752293 None:intergenic 25.0%
!! ATTTTGTTGCATTTTGAACA+TGG + chr3_4:64752275-64752294 None:intergenic 25.0%
! AGAAAAAGAGATACATTTCT+AGG - chr3_4:64752365-64752384 Msa0514090:CDS 25.0%
! AGCAATGAAAATATTGATGA+TGG - chr3_4:64752209-64752228 Msa0514090:CDS 25.0%
! GAAAAAAGAGATACATTTCT+AGG - chr3_4:64752718-64752737 Msa0514090:CDS 25.0%
ACTCATTCATCATCATCAAT+TGG - chr3_4:64751867-64751886 Msa0514090:CDS 30.0%
AGCATGACTTCCAAAATTAT+TGG + chr3_4:64751895-64751914 None:intergenic 30.0%
ATGTTAACAAGAACGATATG+AGG - chr3_4:64752174-64752193 Msa0514090:CDS 30.0%
TTATCAGTACAAGCAATAAC+AGG + chr3_4:64752451-64752470 None:intergenic 30.0%
! TTACATTTTGAACATGGGTA+CGG + chr3_4:64752621-64752640 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG + chr3_4:64752451-64752470 None:intergenic 30.0%
AAGTGGGATAAAAATCAAGT+CGG - chr3_4:64752881-64752900 Msa0514090:CDS 30.0%
ACTCATTCATCATCATCAAT+TGG - chr3_4:64751867-64751886 Msa0514090:CDS 30.0%
AGCATGACTTCCAAAATTAT+TGG + chr3_4:64751895-64751914 None:intergenic 30.0%
ATGTTAACAAGAACGATATG+AGG - chr3_4:64752174-64752193 Msa0514090:CDS 30.0%
TTATCAGTACAAGCAATAAC+AGG + chr3_4:64752451-64752470 None:intergenic 30.0%
! TTACATTTTGAACATGGGTA+CGG + chr3_4:64752621-64752640 None:intergenic 30.0%
TTATCAGTACAAGCAATAAC+AGG + chr3_4:64752451-64752470 None:intergenic 30.0%
AAGTGGGATAAAAATCAAGT+CGG - chr3_4:64752881-64752900 Msa0514090:CDS 30.0%
AGATTGTTTCAATGGATGCA+TGG - chr3_4:64751834-64751853 Msa0514090:CDS 35.0%
!! TAATTTTGGAAGTCATGCTC+CGG - chr3_4:64751896-64751915 Msa0514090:CDS 35.0%
AGATTCTTGAAGGTCAGTTT+GGG - chr3_4:64751937-64751956 Msa0514090:CDS 35.0%
ATTGTGATGATAAGCCTACA+CGG + chr3_4:64752103-64752122 None:intergenic 35.0%
AATATTCCTGTTGCTGATGT+TGG - chr3_4:64752149-64752168 Msa0514090:CDS 35.0%
! TTGCATTTTGAACATGGGTA+CGG + chr3_4:64752269-64752288 None:intergenic 35.0%
CAGATTCAAAAGCTCAATGA+CGG - chr3_4:64752409-64752428 Msa0514090:CDS 35.0%
CAGATTCAAAAGCTCAATGA+CGG - chr3_4:64752409-64752428 Msa0514090:CDS 35.0%
! TTTTTATCCCACTTGGAAGA+GGG + chr3_4:64752875-64752894 None:intergenic 35.0%
! ATTTTTATCCCACTTGGAAG+AGG + chr3_4:64752876-64752895 None:intergenic 35.0%
! GACTTGATTTTTATCCCACT+TGG + chr3_4:64752882-64752901 None:intergenic 35.0%
AGATTGTTTCAATGGATGCA+TGG - chr3_4:64751834-64751853 Msa0514090:CDS 35.0%
!! TAATTTTGGAAGTCATGCTC+CGG - chr3_4:64751896-64751915 Msa0514090:CDS 35.0%
AGATTCTTGAAGGTCAGTTT+GGG - chr3_4:64751937-64751956 Msa0514090:CDS 35.0%
ATTGTGATGATAAGCCTACA+CGG + chr3_4:64752103-64752122 None:intergenic 35.0%
AATATTCCTGTTGCTGATGT+TGG - chr3_4:64752149-64752168 Msa0514090:CDS 35.0%
! TTGCATTTTGAACATGGGTA+CGG + chr3_4:64752269-64752288 None:intergenic 35.0%
CAGATTCAAAAGCTCAATGA+CGG - chr3_4:64752409-64752428 Msa0514090:CDS 35.0%
CAGATTCAAAAGCTCAATGA+CGG - chr3_4:64752409-64752428 Msa0514090:CDS 35.0%
! TTTTTATCCCACTTGGAAGA+GGG + chr3_4:64752875-64752894 None:intergenic 35.0%
! ATTTTTATCCCACTTGGAAG+AGG + chr3_4:64752876-64752895 None:intergenic 35.0%
! GACTTGATTTTTATCCCACT+TGG + chr3_4:64752882-64752901 None:intergenic 35.0%
!! AATTTTGGAAGTCATGCTCC+GGG - chr3_4:64751897-64751916 Msa0514090:CDS 40.0%
!! CAAGAATCTCCATCGCTTTT+AGG + chr3_4:64751926-64751945 None:intergenic 40.0%
AAAGCGATGGAGATTCTTGA+AGG - chr3_4:64751927-64751946 Msa0514090:CDS 40.0%
GAGATTCTTGAAGGTCAGTT+TGG - chr3_4:64751936-64751955 Msa0514090:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG + chr3_4:64752240-64752259 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG + chr3_4:64752241-64752260 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG - chr3_4:64752242-64752261 Msa0514090:intron 40.0%
GTCCATATTTCTTGTGAGGT+AGG + chr3_4:64752247-64752266 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG + chr3_4:64752251-64752270 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG + chr3_4:64752403-64752422 None:intergenic 40.0%
ATCGGAACAAGAACGATATG+AGG - chr3_4:64752526-64752545 Msa0514090:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG + chr3_4:64752240-64752259 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG + chr3_4:64752241-64752260 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG - chr3_4:64752242-64752261 Msa0514090:intron 40.0%
GTCCATATTTCTTGTGAGGT+AGG + chr3_4:64752247-64752266 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG + chr3_4:64752251-64752270 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG + chr3_4:64752403-64752422 None:intergenic 40.0%
! TTTTATCCCACTTGGAAGAG+GGG + chr3_4:64752874-64752893 None:intergenic 40.0%
!! AATTTTGGAAGTCATGCTCC+GGG - chr3_4:64751897-64751916 Msa0514090:CDS 40.0%
!! CAAGAATCTCCATCGCTTTT+AGG + chr3_4:64751926-64751945 None:intergenic 40.0%
AAAGCGATGGAGATTCTTGA+AGG - chr3_4:64751927-64751946 Msa0514090:CDS 40.0%
GAGATTCTTGAAGGTCAGTT+TGG - chr3_4:64751936-64751955 Msa0514090:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG + chr3_4:64752240-64752259 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG + chr3_4:64752241-64752260 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG - chr3_4:64752242-64752261 Msa0514090:intron 40.0%
GTCCATATTTCTTGTGAGGT+AGG + chr3_4:64752247-64752266 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG + chr3_4:64752251-64752270 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG + chr3_4:64752403-64752422 None:intergenic 40.0%
ATCGGAACAAGAACGATATG+AGG - chr3_4:64752526-64752545 Msa0514090:CDS 40.0%
TTTCTTGTGAGGTAGGCTAT+GGG + chr3_4:64752240-64752259 None:intergenic 40.0%
ATTTCTTGTGAGGTAGGCTA+TGG + chr3_4:64752241-64752260 None:intergenic 40.0%
AGCCTACCTCACAAGAAATA+TGG - chr3_4:64752242-64752261 Msa0514090:intron 40.0%
GTCCATATTTCTTGTGAGGT+AGG + chr3_4:64752247-64752266 None:intergenic 40.0%
TACGGTCCATATTTCTTGTG+AGG + chr3_4:64752251-64752270 None:intergenic 40.0%
! AGCTTTTGAATCTGAAGACG+CGG + chr3_4:64752403-64752422 None:intergenic 40.0%
! TTTTATCCCACTTGGAAGAG+GGG + chr3_4:64752874-64752893 None:intergenic 40.0%
!!! TCCATCGCTTTTAGGATTCC+CGG + chr3_4:64751918-64751937 None:intergenic 45.0%
TCTCATCTTGATCTCCGTGT+AGG - chr3_4:64752086-64752105 Msa0514090:CDS 45.0%
ACATCTCCAACATCAGCAAC+AGG + chr3_4:64752158-64752177 None:intergenic 45.0%
ATTGCTCTTACCCCATGCAT+CGG - chr3_4:64752508-64752527 Msa0514090:CDS 45.0%
TCGTTCTTGTTCCGATGCAT+GGG + chr3_4:64752522-64752541 None:intergenic 45.0%
ATCGTTCTTGTTCCGATGCA+TGG + chr3_4:64752523-64752542 None:intergenic 45.0%
!!! TCCATCGCTTTTAGGATTCC+CGG + chr3_4:64751918-64751937 None:intergenic 45.0%
TCTCATCTTGATCTCCGTGT+AGG - chr3_4:64752086-64752105 Msa0514090:CDS 45.0%
ACATCTCCAACATCAGCAAC+AGG + chr3_4:64752158-64752177 None:intergenic 45.0%
ATTGCTCTTACCCCATGCAT+CGG - chr3_4:64752508-64752527 Msa0514090:CDS 45.0%
TCGTTCTTGTTCCGATGCAT+GGG + chr3_4:64752522-64752541 None:intergenic 45.0%
ATCGTTCTTGTTCCGATGCA+TGG + chr3_4:64752523-64752542 None:intergenic 45.0%
!! ATCATTAATATTTATATTAA+TGG + chr3_4:64751997-64752016 None:intergenic 5.0%
!! ATCATTAATATTTATATTAA+TGG + chr3_4:64751997-64752016 None:intergenic 5.0%
TCCGGGAATCCTAAAAGCGA+TGG - chr3_4:64751914-64751933 Msa0514090:CDS 50.0%
CGTTCTTGTTCCGATGCATG+GGG + chr3_4:64752521-64752540 None:intergenic 50.0%
CTCTTACCCCTCTTCCAAGT+GGG - chr3_4:64752865-64752884 Msa0514090:CDS 50.0%
TCCGGGAATCCTAAAAGCGA+TGG - chr3_4:64751914-64751933 Msa0514090:CDS 50.0%
CGTTCTTGTTCCGATGCATG+GGG + chr3_4:64752521-64752540 None:intergenic 50.0%
CTCTTACCCCTCTTCCAAGT+GGG - chr3_4:64752865-64752884 Msa0514090:CDS 50.0%
GCTCTTACCCCTCTTCCAAG+TGG - chr3_4:64752864-64752883 Msa0514090:CDS 55.0%
GCTCTTACCCCTCTTCCAAG+TGG - chr3_4:64752864-64752883 Msa0514090:CDS 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 64751822 64752920 64751822 ID=Msa0514090;Name=Msa0514090
chr3_4 mRNA 64751822 64752920 64751822 ID=Msa0514090-mRNA-1;Parent=Msa0514090;Name=Msa0514090-mRNA-1;_AED=0.07;_eAED=0.10;_QI=0|0|0|1|1|1|2|0|332
chr3_4 exon 64752339 64752920 64752339 ID=Msa0514090-mRNA-1:exon:16330;Parent=Msa0514090-mRNA-1
chr3_4 exon 64751822 64752238 64751822 ID=Msa0514090-mRNA-1:exon:16329;Parent=Msa0514090-mRNA-1
chr3_4 CDS 64752339 64752920 64752339 ID=Msa0514090-mRNA-1:cds;Parent=Msa0514090-mRNA-1
chr3_4 CDS 64751822 64752238 64751822 ID=Msa0514090-mRNA-1:cds;Parent=Msa0514090-mRNA-1
Gene Sequence

>Msa0514090

ATGAACAATAATAGATTGTTTCAATGGATGCATGGTTACAACACAACTCATTCATCATCATCAATTGGTACCAATAATTTTGGAAGTCATGCTCCGGGAATCCTAAAAGCGATGGAGATTCTTGAAGGTCAGTTTGGGCATTTGCATACATCAACATCATCATCGTCATCATCCATTAATATAAATATTAATGATGACATCATCATAAATAACAACAAGAACAACAACATGATGATGACAATGAATACTTCTGCTGCTGATTTTTCTCATCTTGATCTCCGTGTAGGCTTATCATCACAATCACAAGAACTGCCTTCTCAAATGATTAATATTCCTGTTGCTGATGTTGGAGATGTTAACAAGAACGATATGAGGAAGAAGAAGAAGAGCAATGAAAATATTGATGATGGAAGAACCCATAGCCTACCTCACAAGAAATATGGACCGTACCCATGTTCAAAATGCAACAAAATATTTGAAACATCACAAAAATTTGCTAATCATGTTTCATCAAGTCACTACAAGTTCGAAAGTGAAGAAGACAGAAAAAGAGATACATTTCTAGGATTCAGAAGAGACCGCTATGATTGCTCTTACCCCATGCATCGGAACAAGAACGATATGAGGAAGAAGAAGAAGAGCAATGAAAATATTGATGATGGAAGAACCCATAGCCTACCTCACAAGAAATATGGACCGTACCCATGTTCAAAATGTAACAAAATATTTGAAACATCACAAAAATTTGCTAATCATGTTTCATCAAGTCACTACAAGTTCGAAAGTGAAGAAGACAGAAAAAAGAGATACATTTCTAGGATTCAGAAGAGACCGCGTCTTCAGATTCAAAAGCTCAATGACGGAACAACTACTTTTGTTCCTGTTATTGCTTGTACTGATAAGTCACATGCTTCAGTCAATGATGATGATCATAGTATGATTGCTCTTACCCCTCTTCCAAGTGGGATAAAAATCAAGTCGGAGCCTTCAAATAATTAA

Protein sequence

>Msa0514090

MNNNRLFQWMHGYNTTHSSSSIGTNNFGSHAPGILKAMEILEGQFGHLHTSTSSSSSSINININDDIIINNNKNNNMMMTMNTSAADFSHLDLRVGLSSQSQELPSQMINIPVADVGDVNKNDMRKKKKSNENIDDGRTHSLPHKKYGPYPCSKCNKIFETSQKFANHVSSSHYKFESEEDRKRDTFLGFRRDRYDCSYPMHRNKNDMRKKKKSNENIDDGRTHSLPHKKYGPYPCSKCNKIFETSQKFANHVSSSHYKFESEEDRKKRYISRIQKRPRLQIQKLNDGTTTFVPVIACTDKSHASVNDDDHSMIALTPLPSGIKIKSEPSNN*