AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0514300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0514300 MtrunA17_Chr3g0122411 93.960 447 14 1 1 447 1 434 0.0 865
Msa0514300 MtrunA17_Chr8g0377421 48.328 329 124 5 1 321 1 291 1.05e-86 267
Msa0514300 MtrunA17_Chr8g0377401 54.656 247 89 5 1 237 1 234 2.69e-68 218
Msa0514300 MtrunA17_Chr8g0377431 47.748 222 94 6 1 218 1 204 5.61e-48 163
Msa0514300 MtrunA17_Chr5g0420051 41.827 208 112 4 39 240 52 256 6.53e-40 148
Msa0514300 MtrunA17_Chr7g0231911 47.826 161 80 3 93 249 73 233 1.28e-37 139
Msa0514300 MtrunA17_Chr7g0258471 35.862 290 126 7 120 387 91 342 3.09e-37 140
Msa0514300 MtrunA17_Chr8g0360361 70.000 80 24 0 163 242 63 142 5.66e-36 130
Msa0514300 MtrunA17_Chr3g0132091 49.606 127 61 3 151 276 58 182 1.35e-35 134
Msa0514300 MtrunA17_Chr4g0061651 50.781 128 56 2 117 240 74 198 5.04e-35 135
Msa0514300 MtrunA17_Chr4g0046991 55.963 109 32 1 130 238 54 146 1.00e-34 126
Msa0514300 MtrunA17_Chr8g0334671 47.500 160 63 4 118 266 147 296 3.92e-34 130
Msa0514300 MtrunA17_Chr7g0224821 59.341 91 34 1 154 244 69 156 9.95e-33 127
Msa0514300 MtrunA17_Chr8g0386271 56.311 103 42 2 164 266 98 197 1.79e-32 127
Msa0514300 MtrunA17_Chr2g0282521 57.895 95 39 1 148 242 48 141 4.99e-32 119
Msa0514300 MtrunA17_Chr2g0308321 60.000 90 36 0 151 240 157 246 9.15e-31 124
Msa0514300 MtrunA17_Chr1g0194121 56.842 95 41 0 150 244 132 226 3.28e-29 121
Msa0514300 MtrunA17_Chr2g0302791 47.934 121 55 2 134 247 102 221 1.04e-28 119
Msa0514300 MtrunA17_Chr1g0162871 58.621 87 36 0 163 249 102 188 2.48e-28 118
Msa0514300 MtrunA17_Chr8g0377391 46.914 162 75 6 262 412 1 162 6.84e-28 108
Msa0514300 MtrunA17_Chr7g0270541 61.250 80 31 0 165 244 145 224 1.59e-26 113
Msa0514300 MtrunA17_Chr2g0286661 48.315 89 46 0 150 238 88 176 8.53e-22 98.6
Msa0514300 MtrunA17_Chr2g0317041 58.065 62 26 0 150 211 84 145 5.90e-20 86.3
Msa0514300 MtrunA17_Chr1g0175031 46.602 103 49 2 115 211 18 120 5.19e-19 83.2
Msa0514300 MtrunA17_Chr8g0377411 50.000 98 41 3 346 438 3 97 3.80e-14 68.2
Msa0514300 MtrunA17_Chr1g0175021 33.721 172 94 5 212 381 8 161 7.30e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0514300 AT5G43270.3 38.265 392 170 15 1 366 1 346 3.66e-54 186
Msa0514300 AT5G43270.1 38.265 392 170 15 1 366 1 346 3.66e-54 186
Msa0514300 AT5G43270.2 38.265 392 170 15 1 366 1 346 3.66e-54 186
Msa0514300 AT1G27370.6 47.674 258 110 7 1 240 1 251 1.50e-53 183
Msa0514300 AT1G27370.1 47.674 258 110 7 1 240 1 251 6.09e-53 182
Msa0514300 AT1G27370.5 47.674 258 110 7 1 240 1 251 6.09e-53 182
Msa0514300 AT1G27370.3 47.674 258 110 7 1 240 1 251 6.09e-53 182
Msa0514300 AT1G27370.2 47.674 258 110 7 1 240 1 251 6.09e-53 182
Msa0514300 AT1G27370.4 47.674 258 110 7 1 240 1 251 6.09e-53 182
Msa0514300 AT1G27370.7 47.674 258 110 7 1 240 1 251 6.09e-53 182
Msa0514300 AT1G27360.2 38.817 389 197 16 1 366 1 371 6.79e-52 180
Msa0514300 AT1G27360.1 38.817 389 197 16 1 366 1 371 6.79e-52 180
Msa0514300 AT1G27360.3 38.817 389 197 16 1 366 1 371 6.79e-52 180
Msa0514300 AT1G27360.4 38.817 389 197 16 1 366 1 371 6.79e-52 180
Msa0514300 AT2G42200.1 53.543 127 56 1 120 246 32 155 1.72e-35 135
Msa0514300 AT1G69170.1 66.304 92 31 0 148 239 107 198 2.00e-35 135
Msa0514300 AT3G57920.1 52.459 122 45 3 120 241 27 135 5.42e-34 130
Msa0514300 AT1G02065.1 51.969 127 53 2 118 243 147 266 6.34e-33 127
Msa0514300 AT5G50570.1 60.976 82 32 0 163 244 99 180 2.38e-31 124
Msa0514300 AT5G50570.2 60.976 82 32 0 163 244 99 180 2.38e-31 124
Msa0514300 AT5G50670.1 60.976 82 32 0 163 244 99 180 2.38e-31 124
Msa0514300 AT5G50670.2 60.976 82 32 0 163 244 99 180 2.38e-31 124
Msa0514300 AT2G33810.1 59.091 88 33 1 151 238 43 127 1.44e-30 115
Msa0514300 AT1G76580.2 60.674 89 35 0 163 251 112 200 3.94e-30 124
Msa0514300 AT1G76580.3 60.674 89 35 0 163 251 112 200 4.62e-30 124
Msa0514300 AT1G76580.1 60.674 89 35 0 163 251 112 200 7.49e-30 123
Msa0514300 AT1G53160.3 45.299 117 56 1 165 273 54 170 2.37e-29 113
Msa0514300 AT3G60030.1 61.250 80 31 0 165 244 127 206 4.06e-29 121
Msa0514300 AT2G47070.1 50.000 126 56 2 132 256 79 198 8.77e-29 120
Msa0514300 AT1G53160.2 45.385 130 56 4 165 288 54 174 1.51e-28 111
Msa0514300 AT1G53160.1 45.385 130 56 4 165 288 54 174 1.51e-28 111
Msa0514300 AT1G20980.1 60.000 95 37 1 151 244 106 200 2.30e-28 119
Msa0514300 AT3G15270.1 60.811 74 29 0 165 238 63 136 6.30e-28 109
Msa0514300 AT1G02065.2 46.875 96 43 2 118 212 147 235 5.72e-19 86.7
Msa0514300 AT5G18830.3 51.316 76 36 1 165 239 138 213 6.13e-18 87.0
Msa0514300 AT5G18830.2 51.316 76 36 1 165 239 138 213 6.15e-18 87.0
Msa0514300 AT5G18830.1 51.316 76 36 1 165 239 138 213 6.62e-18 87.0
Msa0514300 AT2G47070.2 60.294 68 26 1 190 256 2 69 2.70e-16 82.0

Find 170 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCTCTTTACACTCTTT+TGG 0.140706 3_4:-64990960 Msa0514300:five_prime_UTR
GGTTTAGCTTGATTGGTTTC+TGG 0.141242 3_4:-64990604 Msa0514300:five_prime_UTR
TTTGGATCAGACAAGGTATT+TGG 0.193506 3_4:+64987930 None:intergenic
TTCCAACTAAACACAGATTT+TGG 0.195484 3_4:+64990517 None:intergenic
CTGTGATAAATGTGAATTAT+TGG 0.208468 3_4:-64990419 Msa0514300:five_prime_UTR
GCTAAATTTGAAGTATTCTT+TGG 0.209568 3_4:+64988303 None:intergenic
TGCAGAGGAAGAGGCTTTCT+TGG 0.222637 3_4:+64989231 None:intergenic
TTGATATTGGCTTTCCATTA+TGG 0.227294 3_4:-64990767 Msa0514300:five_prime_UTR
CAAGCTTTCATGATGACTTT+TGG 0.235533 3_4:-64991029 Msa0514300:five_prime_UTR
AGCTTGCTGTTGGGCATGTC+TGG 0.241731 3_4:+64988276 None:intergenic
GTATTCTTTGGAAAAGTAAA+TGG 0.244323 3_4:+64988315 None:intergenic
TCATGCAAATATTAAATTAC+TGG 0.261187 3_4:-64990147 Msa0514300:intron
GGTTTCTGGCATTTATGATA+TGG 0.273991 3_4:-64990590 Msa0514300:five_prime_UTR
GGATTCAATTTCACTGCTTC+AGG 0.274502 3_4:+64988809 None:intergenic
GAGCATAAAATCTTTGGTTT+GGG 0.292731 3_4:+64988251 None:intergenic
CCATTATGGTTTAAAACTTC+AGG 0.298202 3_4:-64990753 Msa0514300:five_prime_UTR
TCCAAACATGTACTCATTAT+AGG 0.303289 3_4:-64990240 Msa0514300:five_prime_UTR
TGAATTAGACATCATACTTA+TGG 0.306180 3_4:-64990182 Msa0514300:five_prime_UTR
AATCTTGGTTCCTTTGCCTT+TGG 0.306346 3_4:+64988161 None:intergenic
TTTAGACGATTCTTTCTTAC+CGG 0.323854 3_4:+64989351 None:intergenic
GCTGAGAAAGCTTGTGATAA+AGG 0.328147 3_4:-64990908 Msa0514300:five_prime_UTR
TGAGCATAAAATCTTTGGTT+TGG 0.329729 3_4:+64988250 None:intergenic
ACCTATAATGAGTACATGTT+TGG 0.334062 3_4:+64990239 None:intergenic
CAATATTCCGGTGACGAAAA+AGG 0.345818 3_4:+64987771 None:intergenic
TTAACATCTTGTGTAGCATT+TGG 0.347549 3_4:+64987912 None:intergenic
GCAAGGGAAACTTGACTCAT+AGG 0.354069 3_4:+64987650 None:intergenic
TAGAAGAGTCTCTTTCCTTA+AGG 0.375177 3_4:+64990804 None:intergenic
AAGTTTAGGTAGTGCATGTT+TGG 0.375544 3_4:-64990368 Msa0514300:five_prime_UTR
CTCTTCTGTCAACCAATTCA+TGG 0.377885 3_4:-64987878 Msa0514300:CDS
TACATCTTAAATGCTGGCTT+TGG 0.377938 3_4:+64990679 None:intergenic
TACTACCACCACAGGCGATT+TGG 0.381836 3_4:+64989069 None:intergenic
TTCTTACCGGTGGAATTGTC+TGG 0.385894 3_4:+64989364 None:intergenic
TGATATGGAGGTTTGGTTGT+TGG 0.388695 3_4:-64990575 Msa0514300:five_prime_UTR
ACATGTACCTGCTACATTGC+TGG 0.389975 3_4:+64989023 None:intergenic
ATTGATAGAATACATATTAC+AGG 0.392874 3_4:-64987963 Msa0514300:CDS
GCATGTTTGGATTGGAGGGT+GGG 0.395614 3_4:-64990355 Msa0514300:five_prime_UTR
AATCTCCCGGGCAATGGGAC+TGG 0.397861 3_4:-64989624 Msa0514300:CDS
GCTGCTGGAGAAGTTTCAAT+TGG 0.402606 3_4:+64989322 None:intergenic
CCATGTTGAAAGTGAAGTTT+AGG 0.402615 3_4:-64990382 Msa0514300:five_prime_UTR
GCATTTATGATATGGAGGTT+TGG 0.408107 3_4:-64990582 Msa0514300:five_prime_UTR
AGCTGCCAGGTGGAAGGTTG+TGG 0.411491 3_4:-64989151 Msa0514300:CDS
ATCTCCCGGGCAATGGGACT+GGG 0.414643 3_4:-64989623 Msa0514300:CDS
CAGCGCCTGTATCTCGTTCT+CGG 0.419065 3_4:-64987721 Msa0514300:CDS
AATTTCACTGCTTCAGGCTG+AGG 0.421915 3_4:+64988815 None:intergenic
ACAATCTACTGATTGGAGTA+TGG 0.424631 3_4:-64989554 Msa0514300:CDS
TGCATGTTTGGATTGGAGGG+TGG 0.426451 3_4:-64990356 Msa0514300:five_prime_UTR
CGTGAAATCTCCCGGGCAAT+GGG 0.426658 3_4:-64989629 Msa0514300:CDS
CAGCATTTAAGATGTAGAAT+AGG 0.427718 3_4:-64990672 Msa0514300:five_prime_UTR
GCTTATGTTGCAACCAGAAA+AGG 0.430624 3_4:+64991051 None:intergenic
GTGAGCAATGGTTCTGCTGC+TGG 0.432022 3_4:+64989307 None:intergenic
GTTGAGGTTGTTGTTAATGT+GGG 0.433390 3_4:-64990473 Msa0514300:five_prime_UTR
CTAGTACATATGAAAAGCTT+TGG 0.437366 3_4:+64990833 None:intergenic
AAGTTTCCCTTGCAACCAGC+TGG 0.440887 3_4:-64987640 Msa0514300:CDS
TAGTGCATGTTTGGATTGGA+GGG 0.445875 3_4:-64990359 Msa0514300:five_prime_UTR
GAATTAGACATCATACTTAT+GGG 0.446634 3_4:-64990181 Msa0514300:five_prime_UTR
ATACACTCCAATCGGACAAC+CGG 0.451604 3_4:-64987825 Msa0514300:CDS
TCTTCTGTCAACCAATTCAT+GGG 0.452114 3_4:-64987877 Msa0514300:CDS
CGAGATACAGGCGCTGGAGC+TGG 0.455430 3_4:+64987728 None:intergenic
ATGTACTAGCAAGTTCCTTA+AGG 0.457613 3_4:-64990819 Msa0514300:five_prime_UTR
TGTTTGGATTGGAGGGTGGG+TGG 0.460095 3_4:-64990352 Msa0514300:five_prime_UTR
CATCAAACTCTGAGAGATCA+TGG 0.461380 3_4:+64988894 None:intergenic
CAACCTTCCACCTGGCAGCT+TGG 0.467365 3_4:+64989154 None:intergenic
AAAGGCAAAGGAACCAAGAT+TGG 0.468272 3_4:-64988159 Msa0514300:CDS
TGTTGAGGTTGTTGTTAATG+TGG 0.470186 3_4:-64990474 Msa0514300:five_prime_UTR
TCGCCTGTGGTGGTAGTAGC+TGG 0.470263 3_4:-64989064 Msa0514300:CDS
TACTTTGAGGATGTTACCAC+TGG 0.470829 3_4:-64989262 Msa0514300:CDS
GTAGTGCATGTTTGGATTGG+AGG 0.471021 3_4:-64990360 Msa0514300:five_prime_UTR
GTTGGGATGTTGATTCCATC+TGG 0.471449 3_4:-64989511 Msa0514300:CDS
CATTTATCACAGCAAATGTC+AGG 0.471850 3_4:+64990430 None:intergenic
CCTTTCTTTCCGCACAAAAG+AGG 0.471950 3_4:+64989205 None:intergenic
TAGGTAGTGCATGTTTGGAT+TGG 0.472523 3_4:-64990363 Msa0514300:five_prime_UTR
CTATTCTACATCTTAAATGC+TGG 0.472880 3_4:+64990673 None:intergenic
TGATCAGTGTGCCAATATTC+CGG 0.473058 3_4:+64987759 None:intergenic
GTAATTTAATATTTGCATGA+AGG 0.476058 3_4:+64990150 None:intergenic
ACAGGTTACAATCTACTGAT+TGG 0.476801 3_4:-64989561 Msa0514300:CDS
TGGTAAAAGATTCTACTTTG+AGG 0.477123 3_4:-64989275 Msa0514300:CDS
AGGGGTGCCGGTTGTCCGAT+TGG 0.477616 3_4:+64987818 None:intergenic
TGTGGTGGTAGTAGCTGGTT+TGG 0.481417 3_4:-64989059 Msa0514300:CDS
TATTCTTTGGAAAAGTAAAT+GGG 0.482035 3_4:+64988316 None:intergenic
TGCCAAAATCTGTGTTTAGT+TGG 0.483513 3_4:-64990519 Msa0514300:five_prime_UTR
ACAACCTGGGACACCAATCT+TGG 0.485267 3_4:+64988146 None:intergenic
ACGTGAAATCTCCCGGGCAA+TGG 0.485685 3_4:-64989630 Msa0514300:CDS
AGCATTCTCATTAATCCAGC+TGG 0.486284 3_4:+64987625 None:intergenic
TAATTTAATATTTGCATGAA+GGG 0.486333 3_4:+64990151 None:intergenic
GAAGATCGAGAAATCAATGT+TGG 0.486543 3_4:-64989529 Msa0514300:CDS
AAGAGGAACTGCAGAGGAAG+AGG 0.487764 3_4:+64989222 None:intergenic
CCTAAACTTCACTTTCAACA+TGG 0.491187 3_4:+64990382 None:intergenic
ATCATATGGACCCCATGAAT+TGG 0.492799 3_4:+64987866 None:intergenic
TTGTCCGATTGGAGTGTATG+AGG 0.494492 3_4:+64987829 None:intergenic
CATTAATCCAGCTGGTTGCA+AGG 0.496505 3_4:+64987633 None:intergenic
GATTGGAGTGTATGAGGGAG+GGG 0.501793 3_4:+64987835 None:intergenic
CTTTGCCTTTGGATATAAAG+TGG 0.501899 3_4:+64988172 None:intergenic
GAGTGGAACGTGAAATCTCC+CGG 0.503280 3_4:-64989637 Msa0514300:CDS
TTGATTCCATCTGGTGGTAG+TGG 0.508737 3_4:-64989502 Msa0514300:CDS
AAGATCGAGAAATCAATGTT+GGG 0.508795 3_4:-64989528 Msa0514300:CDS
CACAAATTCTATGTTTCCGA+TGG 0.510237 3_4:+64989101 None:intergenic
TAATGTGGGGACTCATTCAA+TGG 0.511610 3_4:-64990459 Msa0514300:five_prime_UTR
CAAGGGAAACTTGACTCATA+GGG 0.517421 3_4:+64987651 None:intergenic
CATGCTAAATCCAAGCTGCC+AGG 0.528294 3_4:-64989164 Msa0514300:CDS
GTAACCCGAGAACGAGATAC+AGG 0.528459 3_4:+64987716 None:intergenic
CGATTGGAGTGTATGAGGGA+GGG 0.531040 3_4:+64987834 None:intergenic
TTCTGGCATTTATGATATGG+AGG 0.531477 3_4:-64990587 Msa0514300:five_prime_UTR
TGCAGATGGGAGGCAGACAA+TGG 0.534432 3_4:-64988340 Msa0514300:intron
CCTCCCTCATACACTCCAAT+CGG 0.535964 3_4:-64987833 Msa0514300:CDS
GGAACCAAGATTGGTGTCCC+AGG 0.539374 3_4:-64988150 Msa0514300:intron
AGCGCCTGTATCTCGTTCTC+GGG 0.540985 3_4:-64987720 Msa0514300:CDS
ACATCAATGTTAACTCAAGA+AGG 0.548574 3_4:-64989391 Msa0514300:CDS
AGTAGCATACTGAGGGGTGC+CGG 0.549041 3_4:+64987806 None:intergenic
GCAGATGGGAGGCAGACAAT+GGG 0.549297 3_4:-64988339 Msa0514300:intron
CAAAAGCAGCAGAAACTCAC+AGG 0.550624 3_4:-64989579 Msa0514300:CDS
GGTAAGAAAGAATCGTCTAA+AGG 0.551763 3_4:-64989349 Msa0514300:CDS
TTGGCACACTGATCAACATC+AGG 0.552387 3_4:-64987751 Msa0514300:CDS
TTTGGATATAAAGTGGCCAC+TGG 0.552682 3_4:+64988179 None:intergenic
CCAAGCTTTAGTGTGAGCAA+TGG 0.557830 3_4:+64989295 None:intergenic
AGCTCAAATCTAACAAAGGA+AGG 0.557965 3_4:+64990647 None:intergenic
GGGGACGAAGAGAGAGCTGA+TGG 0.559258 3_4:+64988788 None:intergenic
CTAAACTTCACTTTCAACAT+GGG 0.559810 3_4:+64990383 None:intergenic
ATTGGAGTGTATGAGGGAGG+GGG 0.561383 3_4:+64987836 None:intergenic
AGAATACTTCAAATTTAGCA+TGG 0.561391 3_4:-64988299 Msa0514300:CDS
GAATAATTCATCATCAAATG+AGG 0.562543 3_4:-64989422 Msa0514300:CDS
ATTAATCCAGCTGGTTGCAA+GGG 0.568989 3_4:+64987634 None:intergenic
CTTTAGCAAAAGAGAGGTCG+AGG 0.571109 3_4:+64989128 None:intergenic
AATCCAAGCTGCCAGGTGGA+AGG 0.571632 3_4:-64989157 Msa0514300:CDS
TGTGAATTATTGGAATCTGA+TGG 0.578849 3_4:-64990409 Msa0514300:five_prime_UTR
CGAGGCCACAACCTTCCACC+TGG 0.580184 3_4:+64989146 None:intergenic
CTTGAGATTTATACCATAGG+GGG 0.580458 3_4:+64988769 None:intergenic
AGACGATTCTTTCTTACCGG+TGG 0.582162 3_4:+64989354 None:intergenic
TTAAAGCTCAAATCTAACAA+AGG 0.586460 3_4:+64990643 None:intergenic
AGTGGCCACTTTATATCCAA+AGG 0.587042 3_4:-64988177 Msa0514300:CDS
TTTCTTGGAATGACTTCCAG+TGG 0.587315 3_4:+64989246 None:intergenic
CCATTGCTCACACTAAAGCT+TGG 0.588106 3_4:-64989295 Msa0514300:CDS
TGTCCGATTGGAGTGTATGA+GGG 0.588850 3_4:+64987830 None:intergenic
GTCTTTCAGATCACAATGCA+AGG 0.591028 3_4:-64988847 Msa0514300:CDS
GCATAAAATCTTTGGTTTGG+GGG 0.594228 3_4:+64988253 None:intergenic
AATATGTGATTCATTTGTTG+AGG 0.594917 3_4:-64990489 Msa0514300:five_prime_UTR
TTGACAGAAGAGAGAGAGCA+CGG 0.603893 3_4:+64987889 None:intergenic
AGCATAAAATCTTTGGTTTG+GGG 0.604215 3_4:+64988252 None:intergenic
CCGATTGGAGTGTATGAGGG+AGG 0.604444 3_4:+64987833 None:intergenic
TTGAGGTTGTTGTTAATGTG+GGG 0.607836 3_4:-64990472 Msa0514300:five_prime_UTR
CACTTTATATCCAAAGGCAA+AGG 0.613225 3_4:-64988171 Msa0514300:CDS
CGAGAACGAGATACAGGCGC+TGG 0.614056 3_4:+64987722 None:intergenic
AAACCAGCTACTACCACCAC+AGG 0.617364 3_4:+64989061 None:intergenic
CGCTGAGCAGTAGCATACTG+AGG 0.619947 3_4:+64987798 None:intergenic
TTGCATTGTGATCTGAAAGA+CGG 0.621589 3_4:+64988849 None:intergenic
TGTGAGGACTTGTCATGGAG+TGG 0.623994 3_4:-64989654 Msa0514300:exon
GGTAATCTTTAGCAAAAGAG+AGG 0.625875 3_4:+64989122 None:intergenic
GCTGAGCAGTAGCATACTGA+GGG 0.628917 3_4:+64987799 None:intergenic
GAATTTGTGAGGACTTGTCA+TGG 0.630777 3_4:-64989659 Msa0514300:exon
GAATAGCCACTACCACCAGA+TGG 0.631021 3_4:+64989496 None:intergenic
TCACAGCAAATGTCAGGAGT+CGG 0.631347 3_4:+64990436 None:intergenic
TCATTCCAAATCGCCTGTGG+TGG 0.632811 3_4:-64989074 Msa0514300:CDS
CTTCTGTCAACCAATTCATG+GGG 0.634971 3_4:-64987876 Msa0514300:CDS
AGTGGAACGTGAAATCTCCC+GGG 0.641060 3_4:-64989636 Msa0514300:CDS
ACCCAAAAGAGTGTAAAGAG+AGG 0.641317 3_4:+64990958 None:intergenic
TAGTGAGTTTGACAAAATCA+CGG 0.643134 3_4:+64990267 None:intergenic
ATCTACTGATTGGAGTATGG+AGG 0.643141 3_4:-64989551 Msa0514300:CDS
TTGGAGTGTATGAGGGAGGG+GGG 0.645515 3_4:+64987837 None:intergenic
GGGATGTTGATTCCATCTGG+TGG 0.647072 3_4:-64989508 Msa0514300:CDS
CAGTCATTCCAAATCGCCTG+TGG 0.648152 3_4:-64989077 Msa0514300:CDS
TGTAGCATTTGGATCAGACA+AGG 0.648361 3_4:+64987923 None:intergenic
ATATTCATTCTGCAGATGGG+AGG 0.649420 3_4:-64988350 Msa0514300:intron
GGTTCTCCAGACAATTCCAC+CGG 0.652614 3_4:-64989370 Msa0514300:CDS
TCTTGAGATTTATACCATAG+GGG 0.653113 3_4:+64988768 None:intergenic
GCTAAATCCAAGCTGCCAGG+TGG 0.663879 3_4:-64989161 Msa0514300:CDS
ATGCTATCTGATGATTCCAG+TGG 0.666446 3_4:-64988195 Msa0514300:CDS
TAATGCATGCTTCATCCTCG+AGG 0.674783 3_4:-64989462 Msa0514300:CDS
CATCTGTATATGAATTTGTG+AGG 0.689586 3_4:-64989670 Msa0514300:five_prime_UTR
GCACAAAAGAGGAACTGCAG+AGG 0.694164 3_4:+64989216 None:intergenic
CTGAGCAGTAGCATACTGAG+GGG 0.712599 3_4:+64987800 None:intergenic
AAAAGAAAATGACAATGACA+TGG 0.723335 3_4:-64991215 Msa0514300:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACTTTTATTTTTATTTTTG+TGG - chr3_4:64987708-64987727 Msa0514300:CDS 10.0%
!!! TGATTTTCTTTTTCTTTTTT+GGG - chr3_4:64987684-64987703 Msa0514300:CDS 15.0%
!!! TTTTTGTTTTTTAGAGTAAT+AGG - chr3_4:64988017-64988036 Msa0514300:intron 15.0%
!!! TTTTGTTTTTTAGAGTAATA+GGG - chr3_4:64988018-64988037 Msa0514300:intron 15.0%
!!! TACATAATAGATTTTGATAT+TGG - chr3_4:64988096-64988115 Msa0514300:intron 15.0%
!!! ATTATTTTTTTGGTACAATT+TGG + chr3_4:64988150-64988169 None:intergenic 15.0%
!!! GGTACAAAATATTATTTTTT+TGG + chr3_4:64988160-64988179 None:intergenic 15.0%
!! TAATTTAATATTTGCATGAA+GGG + chr3_4:64988728-64988747 None:intergenic 15.0%
!!! ATTAAATTTAGTTTGTTCAA+TGG - chr3_4:64990794-64990813 Msa0514300:five_prime_UTR 15.0%
!!! GTGATTTTCTTTTTCTTTTT+TGG - chr3_4:64987683-64987702 Msa0514300:CDS 20.0%
!!! GTTATTATTATTTCCTTTTC+TGG - chr3_4:64987812-64987831 Msa0514300:CDS 20.0%
!!! AAAAAGCATCTTTTTTTCAT+GGG + chr3_4:64987878-64987897 None:intergenic 20.0%
!!! AAAAAAGCATCTTTTTTTCA+TGG + chr3_4:64987879-64987898 None:intergenic 20.0%
!!! TTTGTTTTTTAGAGTAATAG+GGG - chr3_4:64988019-64988038 Msa0514300:intron 20.0%
!!! TTTAAAAAAGATGCTTTTTG+AGG - chr3_4:64988251-64988270 Msa0514300:CDS 20.0%
!! GTAATTTAATATTTGCATGA+AGG + chr3_4:64988729-64988748 None:intergenic 20.0%
!! TCATGCAAATATTAAATTAC+TGG - chr3_4:64988729-64988748 Msa0514300:intron 20.0%
!!! GTAAACATTTTACATTAGTA+GGG - chr3_4:64988751-64988770 Msa0514300:intron 20.0%
!! AGAATTTATACAGATATACT+TGG - chr3_4:64989027-64989046 Msa0514300:intron 20.0%
!! AACTATACAAGATAATAACA+AGG + chr3_4:64989118-64989137 None:intergenic 20.0%
!! AACTACTAGTCAAATAATAA+TGG + chr3_4:64989173-64989192 None:intergenic 20.0%
!!! TTGGTTTGTTTAGAATAAAT+TGG - chr3_4:64989923-64989942 Msa0514300:intron 20.0%
!!! AACTTAAACTATGATTTTGA+AGG - chr3_4:64989957-64989976 Msa0514300:intron 20.0%
!! TTATGAGAGAATTATAAGAT+TGG - chr3_4:64990229-64990248 Msa0514300:five_prime_UTR 20.0%
!!! TTAGAATTTCTTTTGAGAAA+TGG + chr3_4:64990269-64990288 None:intergenic 20.0%
!!! TACTCTATGACTATATTTTT+GGG + chr3_4:64990375-64990394 None:intergenic 20.0%
!!! ATACTCTATGACTATATTTT+TGG + chr3_4:64990376-64990395 None:intergenic 20.0%
!!! TATTCTTTGGAAAAGTAAAT+GGG + chr3_4:64990563-64990582 None:intergenic 20.0%
!! TTTAATAAGAAATCATGACT+CGG + chr3_4:64990780-64990799 None:intergenic 20.0%
!! ATTGATAGAATACATATTAC+AGG - chr3_4:64990913-64990932 Msa0514300:five_prime_UTR 20.0%
! AAAAGAAAATGACAATGACA+TGG - chr3_4:64987661-64987680 Msa0514300:CDS 25.0%
! TTAAAGCTCAAATCTAACAA+AGG + chr3_4:64988236-64988255 None:intergenic 25.0%
!! AATATGTGATTCATTTGTTG+AGG - chr3_4:64988387-64988406 Msa0514300:intron 25.0%
! CTGTGATAAATGTGAATTAT+TGG - chr3_4:64988457-64988476 Msa0514300:intron 25.0%
!! GTAGCTTTTGCAAAAATTTA+CGG - chr3_4:64988579-64988598 Msa0514300:intron 25.0%
! TGAATTAGACATCATACTTA+TGG - chr3_4:64988694-64988713 Msa0514300:intron 25.0%
! GAATTAGACATCATACTTAT+GGG - chr3_4:64988695-64988714 Msa0514300:intron 25.0%
!! GGTAAACATTTTACATTAGT+AGG - chr3_4:64988750-64988769 Msa0514300:intron 25.0%
!! ATTTTACATTAGTAGGGTTT+AGG - chr3_4:64988757-64988776 Msa0514300:intron 25.0%
!! TTTTACATTAGTAGGGTTTA+GGG - chr3_4:64988758-64988777 Msa0514300:intron 25.0%
! AAAATACCCTTGTAATTCTT+TGG - chr3_4:64988801-64988820 Msa0514300:CDS 25.0%
! TTTAGGAATTAGTAGTTCAT+AGG - chr3_4:64988940-64988959 Msa0514300:intron 25.0%
! GAATAATTCATCATCAAATG+AGG - chr3_4:64989454-64989473 Msa0514300:CDS 25.0%
! TTCTTGAGATTTATACCATA+GGG + chr3_4:64990112-64990131 None:intergenic 25.0%
! ATTCTTGAGATTTATACCAT+AGG + chr3_4:64990113-64990132 None:intergenic 25.0%
! TACAACCAATAACATATCAA+TGG + chr3_4:64990295-64990314 None:intergenic 25.0%
!! ATATGTTATTGGTTGTATGA+AGG - chr3_4:64990298-64990317 Msa0514300:five_prime_UTR 25.0%
! TTCAGTTTCTTTGTAAGAAA+TGG - chr3_4:64990395-64990414 Msa0514300:five_prime_UTR 25.0%
! TTTAATATTCATTCTGCAGA+TGG - chr3_4:64990522-64990541 Msa0514300:five_prime_UTR 25.0%
! TTAATATTCATTCTGCAGAT+GGG - chr3_4:64990523-64990542 Msa0514300:five_prime_UTR 25.0%
!! GTATTCTTTGGAAAAGTAAA+TGG + chr3_4:64990564-64990583 None:intergenic 25.0%
!! GCTAAATTTGAAGTATTCTT+TGG + chr3_4:64990576-64990595 None:intergenic 25.0%
! AGAATACTTCAAATTTAGCA+TGG - chr3_4:64990577-64990596 Msa0514300:five_prime_UTR 25.0%
!!! AGTTTTGAGCATAAAATCTT+TGG + chr3_4:64990634-64990653 None:intergenic 25.0%
!!! TAACAGTTGTTTCTACAAAT+AGG + chr3_4:64990888-64990907 None:intergenic 25.0%
!! TATTTTTGTGGATGTGTGAT+TGG - chr3_4:64987720-64987739 Msa0514300:CDS 30.0%
!!! ATGAGTGCTATTTTTGTTGA+AGG - chr3_4:64987773-64987792 Msa0514300:CDS 30.0%
!!! TGAGTGCTATTTTTGTTGAA+GGG - chr3_4:64987774-64987793 Msa0514300:CDS 30.0%
CTAGTACATATGAAAAGCTT+TGG + chr3_4:64988046-64988065 None:intergenic 30.0%
!! TTGATATTGGCTTTCCATTA+TGG - chr3_4:64988109-64988128 Msa0514300:intron 30.0%
!!! CCTGAAGTTTTAAACCATAA+TGG + chr3_4:64988126-64988145 None:intergenic 30.0%
! CCATTATGGTTTAAAACTTC+AGG - chr3_4:64988123-64988142 Msa0514300:intron 30.0%
TTGTACCAAAAAAACACTTC+AGG - chr3_4:64988173-64988192 Msa0514300:CDS 30.0%
CTATTCTACATCTTAAATGC+TGG + chr3_4:64988206-64988225 None:intergenic 30.0%
! CAGCATTTAAGATGTAGAAT+AGG - chr3_4:64988204-64988223 Msa0514300:CDS 30.0%
!!! TTTTTGAGGTTTAGCTTGAT+TGG - chr3_4:64988265-64988284 Msa0514300:CDS 30.0%
! TTCCAACTAAACACAGATTT+TGG + chr3_4:64988362-64988381 None:intergenic 30.0%
TGTGAATTATTGGAATCTGA+TGG - chr3_4:64988467-64988486 Msa0514300:intron 30.0%
CTAAACTTCACTTTCAACAT+GGG + chr3_4:64988496-64988515 None:intergenic 30.0%
TAGTGAGTTTGACAAAATCA+CGG + chr3_4:64988612-64988631 None:intergenic 30.0%
TCCAAACATGTACTCATTAT+AGG - chr3_4:64988636-64988655 Msa0514300:intron 30.0%
ACCTATAATGAGTACATGTT+TGG + chr3_4:64988640-64988659 None:intergenic 30.0%
TAGTTGTAGTAGAACTATCT+TGG - chr3_4:64988841-64988860 Msa0514300:CDS 30.0%
TATAATCCATGTCTTGCTTT+AGG - chr3_4:64988923-64988942 Msa0514300:intron 30.0%
!!! TCGTACTGTTTTTAAGTGAT+TGG - chr3_4:64988999-64989018 Msa0514300:intron 30.0%
CATCTGTATATGAATTTGTG+AGG - chr3_4:64989206-64989225 Msa0514300:CDS 30.0%
AAGATCGAGAAATCAATGTT+GGG - chr3_4:64989348-64989367 Msa0514300:CDS 30.0%
ACATCAATGTTAACTCAAGA+AGG - chr3_4:64989485-64989504 Msa0514300:CDS 30.0%
TTTAGACGATTCTTTCTTAC+CGG + chr3_4:64989528-64989547 None:intergenic 30.0%
TGGTAAAAGATTCTACTTTG+AGG - chr3_4:64989601-64989620 Msa0514300:CDS 30.0%
! CTTTTGCTAAAGATTACCAT+CGG - chr3_4:64989759-64989778 Msa0514300:intron 30.0%
TCTAAACAAACCAAAAAGAC+AGG + chr3_4:64989917-64989936 None:intergenic 30.0%
TCTTGAGATTTATACCATAG+GGG + chr3_4:64990111-64990130 None:intergenic 30.0%
! AAGGCTAATTGATCTTTTAG+GGG + chr3_4:64990140-64990159 None:intergenic 30.0%
! CAAGGCTAATTGATCTTTTA+GGG + chr3_4:64990141-64990160 None:intergenic 30.0%
! ACAAGGCTAATTGATCTTTT+AGG + chr3_4:64990142-64990161 None:intergenic 30.0%
TAAACTAATGAAGCAAGACA+AGG + chr3_4:64990159-64990178 None:intergenic 30.0%
TGAATGGCGATATCTTAAAA+AGG - chr3_4:64990206-64990225 Msa0514300:five_prime_UTR 30.0%
AACCTCCATTGATATGTTAT+TGG - chr3_4:64990287-64990306 Msa0514300:five_prime_UTR 30.0%
AACCAATAACATATCAATGG+AGG + chr3_4:64990292-64990311 None:intergenic 30.0%
!! AGCATAAAATCTTTGGTTTG+GGG + chr3_4:64990627-64990646 None:intergenic 30.0%
! GAGCATAAAATCTTTGGTTT+GGG + chr3_4:64990628-64990647 None:intergenic 30.0%
TGAGCATAAAATCTTTGGTT+TGG + chr3_4:64990629-64990648 None:intergenic 30.0%
!!! ACAAACTTGCTGAAGTTTTT+TGG + chr3_4:64990657-64990676 None:intergenic 30.0%
TTAAGATTAGAGAACAACCT+GGG + chr3_4:64990746-64990765 None:intergenic 30.0%
! TTTAAGATTAGAGAACAACC+TGG + chr3_4:64990747-64990766 None:intergenic 30.0%
TGAAACATGCAATAAACTAG+AGG + chr3_4:64990846-64990865 None:intergenic 30.0%
TTAACATCTTGTGTAGCATT+TGG + chr3_4:64990967-64990986 None:intergenic 30.0%
! CAAGCTTTCATGATGACTTT+TGG - chr3_4:64987847-64987866 Msa0514300:CDS 35.0%
! TTCCTCTCTTTACACTCTTT+TGG - chr3_4:64987916-64987935 Msa0514300:CDS 35.0%
! TCCTCTCTTTACACTCTTTT+GGG - chr3_4:64987917-64987936 Msa0514300:CDS 35.0%
ATGTACTAGCAAGTTCCTTA+AGG - chr3_4:64988057-64988076 Msa0514300:intron 35.0%
TAGAAGAGTCTCTTTCCTTA+AGG + chr3_4:64988075-64988094 None:intergenic 35.0%
!!! TTGGTCCTGAAGTGTTTTTT+TGG + chr3_4:64988181-64988200 None:intergenic 35.0%
!! TACATCTTAAATGCTGGCTT+TGG + chr3_4:64988200-64988219 None:intergenic 35.0%
AGCTCAAATCTAACAAAGGA+AGG + chr3_4:64988232-64988251 None:intergenic 35.0%
!! GGTTTCTGGCATTTATGATA+TGG - chr3_4:64988286-64988305 Msa0514300:CDS 35.0%
TTCTGGCATTTATGATATGG+AGG - chr3_4:64988289-64988308 Msa0514300:CDS 35.0%
GCATTTATGATATGGAGGTT+TGG - chr3_4:64988294-64988313 Msa0514300:CDS 35.0%
TGCCAAAATCTGTGTTTAGT+TGG - chr3_4:64988357-64988376 Msa0514300:intron 35.0%
! TGTTGAGGTTGTTGTTAATG+TGG - chr3_4:64988402-64988421 Msa0514300:intron 35.0%
! GTTGAGGTTGTTGTTAATGT+GGG - chr3_4:64988403-64988422 Msa0514300:intron 35.0%
! TTGAGGTTGTTGTTAATGTG+GGG - chr3_4:64988404-64988423 Msa0514300:intron 35.0%
CATTTATCACAGCAAATGTC+AGG + chr3_4:64988449-64988468 None:intergenic 35.0%
CCTAAACTTCACTTTCAACA+TGG + chr3_4:64988497-64988516 None:intergenic 35.0%
CCATGTTGAAAGTGAAGTTT+AGG - chr3_4:64988494-64988513 Msa0514300:intron 35.0%
AAGTTTAGGTAGTGCATGTT+TGG - chr3_4:64988508-64988527 Msa0514300:intron 35.0%
TACCCTTGTAATTCTTTGGT+TGG - chr3_4:64988805-64988824 Msa0514300:CDS 35.0%
CTCCAACCAAAGAATTACAA+GGG + chr3_4:64988810-64988829 None:intergenic 35.0%
CTTGTAATTCTTTGGTTGGA+GGG - chr3_4:64988809-64988828 Msa0514300:CDS 35.0%
TTGTAATTCTTTGGTTGGAG+GGG - chr3_4:64988810-64988829 Msa0514300:CDS 35.0%
!! ATCTTGGTTTGCATTTGCAT+AGG - chr3_4:64988857-64988876 Msa0514300:CDS 35.0%
CTAATTCCTAAAGCAAGACA+TGG + chr3_4:64988932-64988951 None:intergenic 35.0%
ACAGGTTACAATCTACTGAT+TGG - chr3_4:64989315-64989334 Msa0514300:CDS 35.0%
! ACAATCTACTGATTGGAGTA+TGG - chr3_4:64989322-64989341 Msa0514300:CDS 35.0%
GAAGATCGAGAAATCAATGT+TGG - chr3_4:64989347-64989366 Msa0514300:CDS 35.0%
GGTAAGAAAGAATCGTCTAA+AGG - chr3_4:64989527-64989546 Msa0514300:CDS 35.0%
GGTAATCTTTAGCAAAAGAG+AGG + chr3_4:64989757-64989776 None:intergenic 35.0%
CACAAATTCTATGTTTCCGA+TGG + chr3_4:64989778-64989797 None:intergenic 35.0%
!! CAATTAGCTTCCTGTCTTTT+TGG - chr3_4:64989904-64989923 Msa0514300:intron 35.0%
TTGCATTGTGATCTGAAAGA+CGG + chr3_4:64990030-64990049 None:intergenic 35.0%
CTTGAGATTTATACCATAGG+GGG + chr3_4:64990110-64990129 None:intergenic 35.0%
!! CTAATTGATCTTTTAGGGGA+GGG + chr3_4:64990136-64990155 None:intergenic 35.0%
!!! TTGCCATTTTGTTTCTCGTT+TGG - chr3_4:64990181-64990200 Msa0514300:five_prime_UTR 35.0%
!!! TGCCATTTTGTTTCTCGTTT+GGG - chr3_4:64990182-64990201 Msa0514300:five_prime_UTR 35.0%
ATCCCAAACGAGAAACAAAA+TGG + chr3_4:64990187-64990206 None:intergenic 35.0%
!! GCATAAAATCTTTGGTTTGG+GGG + chr3_4:64990626-64990645 None:intergenic 35.0%
CTTTGCCTTTGGATATAAAG+TGG + chr3_4:64990707-64990726 None:intergenic 35.0%
CACTTTATATCCAAAGGCAA+AGG - chr3_4:64990705-64990724 Msa0514300:five_prime_UTR 35.0%
! AATAAGAAATCATGACTCGG+AGG + chr3_4:64990777-64990796 None:intergenic 35.0%
!! CAGTTGTTTCTACAAATAGG+AGG + chr3_4:64990885-64990904 None:intergenic 35.0%
! TTTGGATCAGACAAGGTATT+TGG + chr3_4:64990949-64990968 None:intergenic 35.0%
TCTTCTGTCAACCAATTCAT+GGG - chr3_4:64990999-64991018 Msa0514300:five_prime_UTR 35.0%
GCTTATGTTGCAACCAGAAA+AGG + chr3_4:64987828-64987847 None:intergenic 40.0%
! ACCCAAAAGAGTGTAAAGAG+AGG + chr3_4:64987921-64987940 None:intergenic 40.0%
GCTGAGAAAGCTTGTGATAA+AGG - chr3_4:64987968-64987987 Msa0514300:CDS 40.0%
!! GGTTTAGCTTGATTGGTTTC+TGG - chr3_4:64988272-64988291 Msa0514300:CDS 40.0%
! TGATATGGAGGTTTGGTTGT+TGG - chr3_4:64988301-64988320 Msa0514300:CDS 40.0%
TAATGTGGGGACTCATTCAA+TGG - chr3_4:64988417-64988436 Msa0514300:intron 40.0%
TAGGTAGTGCATGTTTGGAT+TGG - chr3_4:64988513-64988532 Msa0514300:intron 40.0%
! TAGTGCATGTTTGGATTGGA+GGG - chr3_4:64988517-64988536 Msa0514300:intron 40.0%
CATTAGTAGGGTTTAGGGAT+TGG - chr3_4:64988763-64988782 Msa0514300:intron 40.0%
CCTCCAACCAAAGAATTACA+AGG + chr3_4:64988811-64988830 None:intergenic 40.0%
CCTTGTAATTCTTTGGTTGG+AGG - chr3_4:64988808-64988827 Msa0514300:CDS 40.0%
ATCACTTTGCATTTCCTGCT+AGG + chr3_4:64988884-64988903 None:intergenic 40.0%
CCAGAGTAATGATTGGAAGA+AGG + chr3_4:64989065-64989084 None:intergenic 40.0%
CCTTCTTCCAATCATTACTC+TGG - chr3_4:64989062-64989081 Msa0514300:CDS 40.0%
GCAGAAACCAGAGTAATGAT+TGG + chr3_4:64989072-64989091 None:intergenic 40.0%
GAATTTGTGAGGACTTGTCA+TGG - chr3_4:64989217-64989236 Msa0514300:CDS 40.0%
! ATCTACTGATTGGAGTATGG+AGG - chr3_4:64989325-64989344 Msa0514300:CDS 40.0%
! TACTTTGAGGATGTTACCAC+TGG - chr3_4:64989614-64989633 Msa0514300:CDS 40.0%
TTTCTTGGAATGACTTCCAG+TGG + chr3_4:64989633-64989652 None:intergenic 40.0%
! CATCAAACTCTGAGAGATCA+TGG + chr3_4:64989985-64990004 None:intergenic 40.0%
GTCTTTCAGATCACAATGCA+AGG - chr3_4:64990029-64990048 Msa0514300:intron 40.0%
GGATTCAATTTCACTGCTTC+AGG + chr3_4:64990070-64990089 None:intergenic 40.0%
!! GCTAATTGATCTTTTAGGGG+AGG + chr3_4:64990137-64990156 None:intergenic 40.0%
! TGTTTCTCGTTTGGGATGAA+TGG - chr3_4:64990190-64990209 Msa0514300:five_prime_UTR 40.0%
ATATTCATTCTGCAGATGGG+AGG - chr3_4:64990526-64990545 Msa0514300:five_prime_UTR 40.0%
ATGCTATCTGATGATTCCAG+TGG - chr3_4:64990681-64990700 Msa0514300:five_prime_UTR 40.0%
TTTGGATATAAAGTGGCCAC+TGG + chr3_4:64990700-64990719 None:intergenic 40.0%
! AGTGGCCACTTTATATCCAA+AGG - chr3_4:64990699-64990718 Msa0514300:five_prime_UTR 40.0%
AATCTTGGTTCCTTTGCCTT+TGG + chr3_4:64990718-64990737 None:intergenic 40.0%
AAAGGCAAAGGAACCAAGAT+TGG - chr3_4:64990717-64990736 Msa0514300:five_prime_UTR 40.0%
TGTAGCATTTGGATCAGACA+AGG + chr3_4:64990956-64990975 None:intergenic 40.0%
CTCTTCTGTCAACCAATTCA+TGG - chr3_4:64990998-64991017 Msa0514300:five_prime_UTR 40.0%
CTTCTGTCAACCAATTCATG+GGG - chr3_4:64991000-64991019 Msa0514300:five_prime_UTR 40.0%
ATCATATGGACCCCATGAAT+TGG + chr3_4:64991013-64991032 None:intergenic 40.0%
CAATATTCCGGTGACGAAAA+AGG + chr3_4:64991108-64991127 None:intergenic 40.0%
!! CTTTTTCGTCACCGGAATAT+TGG - chr3_4:64991106-64991125 Msa0514300:five_prime_UTR 40.0%
TGATCAGTGTGCCAATATTC+CGG + chr3_4:64991120-64991139 None:intergenic 40.0%
CAAGGGAAACTTGACTCATA+GGG + chr3_4:64991228-64991247 None:intergenic 40.0%
TCACAGCAAATGTCAGGAGT+CGG + chr3_4:64988443-64988462 None:intergenic 45.0%
! GTAGTGCATGTTTGGATTGG+AGG - chr3_4:64988516-64988535 Msa0514300:intron 45.0%
TAATTCTTTGGTTGGAGGGG+TGG - chr3_4:64988813-64988832 Msa0514300:CDS 45.0%
CAAAAGCAGCAGAAACTCAC+AGG - chr3_4:64989297-64989316 Msa0514300:CDS 45.0%
!! GTTGGGATGTTGATTCCATC+TGG - chr3_4:64989365-64989384 Msa0514300:CDS 45.0%
TTGATTCCATCTGGTGGTAG+TGG - chr3_4:64989374-64989393 Msa0514300:CDS 45.0%
TAATGCATGCTTCATCCTCG+AGG - chr3_4:64989414-64989433 Msa0514300:CDS 45.0%
TTCTTACCGGTGGAATTGTC+TGG + chr3_4:64989515-64989534 None:intergenic 45.0%
AGACGATTCTTTCTTACCGG+TGG + chr3_4:64989525-64989544 None:intergenic 45.0%
GCTGCTGGAGAAGTTTCAAT+TGG + chr3_4:64989557-64989576 None:intergenic 45.0%
! CCAAGCTTTAGTGTGAGCAA+TGG + chr3_4:64989584-64989603 None:intergenic 45.0%
CCATTGCTCACACTAAAGCT+TGG - chr3_4:64989581-64989600 Msa0514300:CDS 45.0%
! TCTGCAGTTCCTCTTTTGTG+CGG - chr3_4:64989662-64989681 Msa0514300:exon 45.0%
CCTTTCTTTCCGCACAAAAG+AGG + chr3_4:64989674-64989693 None:intergenic 45.0%
! CCTCTTTTGTGCGGAAAGAA+AGG - chr3_4:64989671-64989690 Msa0514300:five_prime_UTR 45.0%
CTTTAGCAAAAGAGAGGTCG+AGG + chr3_4:64989751-64989770 None:intergenic 45.0%
! GATTTTGCCAGCAATGTAGC+AGG - chr3_4:64989846-64989865 Msa0514300:intron 45.0%
ACATGTACCTGCTACATTGC+TGG + chr3_4:64989856-64989875 None:intergenic 45.0%
AATTTCACTGCTTCAGGCTG+AGG + chr3_4:64990064-64990083 None:intergenic 45.0%
TTGACAGAAGAGAGAGAGCA+CGG + chr3_4:64990990-64991009 None:intergenic 45.0%
TGTCCGATTGGAGTGTATGA+GGG + chr3_4:64991049-64991068 None:intergenic 45.0%
TTGTCCGATTGGAGTGTATG+AGG + chr3_4:64991050-64991069 None:intergenic 45.0%
ATACACTCCAATCGGACAAC+CGG - chr3_4:64991051-64991070 Msa0514300:five_prime_UTR 45.0%
TTGGCACACTGATCAACATC+AGG - chr3_4:64991125-64991144 Msa0514300:five_prime_UTR 45.0%
GCAAGGGAAACTTGACTCAT+AGG + chr3_4:64991229-64991248 None:intergenic 45.0%
! TGCATGTTTGGATTGGAGGG+TGG - chr3_4:64988520-64988539 Msa0514300:intron 50.0%
! GCATGTTTGGATTGGAGGGT+GGG - chr3_4:64988521-64988540 Msa0514300:intron 50.0%
GCATTTGCATAGGTCCTAGC+AGG - chr3_4:64988867-64988886 Msa0514300:CDS 50.0%
TGTGAGGACTTGTCATGGAG+TGG - chr3_4:64989222-64989241 Msa0514300:CDS 50.0%
GAGTGGAACGTGAAATCTCC+CGG - chr3_4:64989239-64989258 Msa0514300:CDS 50.0%
AGTGGAACGTGAAATCTCCC+GGG - chr3_4:64989240-64989259 Msa0514300:CDS 50.0%
!! GGGATGTTGATTCCATCTGG+TGG - chr3_4:64989368-64989387 Msa0514300:CDS 50.0%
GAATAGCCACTACCACCAGA+TGG + chr3_4:64989383-64989402 None:intergenic 50.0%
! AGCAGATTTTGAGCTCCTCG+AGG + chr3_4:64989432-64989451 None:intergenic 50.0%
GGTTCTCCAGACAATTCCAC+CGG - chr3_4:64989506-64989525 Msa0514300:CDS 50.0%
TGCAGAGGAAGAGGCTTTCT+TGG + chr3_4:64989648-64989667 None:intergenic 50.0%
AAGAGGAACTGCAGAGGAAG+AGG + chr3_4:64989657-64989676 None:intergenic 50.0%
GCACAAAAGAGGAACTGCAG+AGG + chr3_4:64989663-64989682 None:intergenic 50.0%
CATGCTAAATCCAAGCTGCC+AGG - chr3_4:64989712-64989731 Msa0514300:intron 50.0%
CAGTCATTCCAAATCGCCTG+TGG - chr3_4:64989799-64989818 Msa0514300:intron 50.0%
TCATTCCAAATCGCCTGTGG+TGG - chr3_4:64989802-64989821 Msa0514300:intron 50.0%
TACTACCACCACAGGCGATT+TGG + chr3_4:64989810-64989829 None:intergenic 50.0%
AAACCAGCTACTACCACCAC+AGG + chr3_4:64989818-64989837 None:intergenic 50.0%
TGTGGTGGTAGTAGCTGGTT+TGG - chr3_4:64989817-64989836 Msa0514300:intron 50.0%
ACAACCTGGGACACCAATCT+TGG + chr3_4:64990733-64990752 None:intergenic 50.0%
ATTGGAGTGTATGAGGGAGG+GGG + chr3_4:64991043-64991062 None:intergenic 50.0%
GATTGGAGTGTATGAGGGAG+GGG + chr3_4:64991044-64991063 None:intergenic 50.0%
CGATTGGAGTGTATGAGGGA+GGG + chr3_4:64991045-64991064 None:intergenic 50.0%
CCTCCCTCATACACTCCAAT+CGG - chr3_4:64991043-64991062 Msa0514300:five_prime_UTR 50.0%
CTGAGCAGTAGCATACTGAG+GGG + chr3_4:64991079-64991098 None:intergenic 50.0%
GCTGAGCAGTAGCATACTGA+GGG + chr3_4:64991080-64991099 None:intergenic 50.0%
GTAACCCGAGAACGAGATAC+AGG + chr3_4:64991163-64991182 None:intergenic 50.0%
AAGTTTCCCTTGCAACCAGC+TGG - chr3_4:64991236-64991255 Msa0514300:five_prime_UTR 50.0%
! TGTTTGGATTGGAGGGTGGG+TGG - chr3_4:64988524-64988543 Msa0514300:intron 55.0%
ACGTGAAATCTCCCGGGCAA+TGG - chr3_4:64989246-64989265 Msa0514300:CDS 55.0%
CGTGAAATCTCCCGGGCAAT+GGG - chr3_4:64989247-64989266 Msa0514300:CDS 55.0%
! TTTTCCCAGTCCCATTGCCC+GGG + chr3_4:64989260-64989279 None:intergenic 55.0%
! GTTTTCCCAGTCCCATTGCC+CGG + chr3_4:64989261-64989280 None:intergenic 55.0%
! GTGAGCAATGGTTCTGCTGC+TGG + chr3_4:64989572-64989591 None:intergenic 55.0%
GCTAAATCCAAGCTGCCAGG+TGG - chr3_4:64989715-64989734 Msa0514300:intron 55.0%
AATCCAAGCTGCCAGGTGGA+AGG - chr3_4:64989719-64989738 Msa0514300:intron 55.0%
TGCAGATGGGAGGCAGACAA+TGG - chr3_4:64990536-64990555 Msa0514300:five_prime_UTR 55.0%
GCAGATGGGAGGCAGACAAT+GGG - chr3_4:64990537-64990556 Msa0514300:five_prime_UTR 55.0%
!! AGCTTGCTGTTGGGCATGTC+TGG + chr3_4:64990603-64990622 None:intergenic 55.0%
!! GTTTGGGGGAGCTTGCTGTT+GGG + chr3_4:64990612-64990631 None:intergenic 55.0%
! GGAACCAAGATTGGTGTCCC+AGG - chr3_4:64990726-64990745 Msa0514300:five_prime_UTR 55.0%
! GAGGGGGGCTTGGTATCATA+TGG + chr3_4:64991027-64991046 None:intergenic 55.0%
TTGGAGTGTATGAGGGAGGG+GGG + chr3_4:64991042-64991061 None:intergenic 55.0%
CCGATTGGAGTGTATGAGGG+AGG + chr3_4:64991046-64991065 None:intergenic 55.0%
! AGTAGCATACTGAGGGGTGC+CGG + chr3_4:64991073-64991092 None:intergenic 55.0%
CGCTGAGCAGTAGCATACTG+AGG + chr3_4:64991081-64991100 None:intergenic 55.0%
!! GCGCTCACCTTTTTCGTCAC+CGG - chr3_4:64991098-64991117 Msa0514300:five_prime_UTR 55.0%
CAGCGCCTGTATCTCGTTCT+CGG - chr3_4:64991155-64991174 Msa0514300:five_prime_UTR 55.0%
AGCGCCTGTATCTCGTTCTC+GGG - chr3_4:64991156-64991175 Msa0514300:five_prime_UTR 55.0%
AATCTCCCGGGCAATGGGAC+TGG - chr3_4:64989252-64989271 Msa0514300:CDS 60.0%
ATCTCCCGGGCAATGGGACT+GGG - chr3_4:64989253-64989272 Msa0514300:CDS 60.0%
CAACCTTCCACCTGGCAGCT+TGG + chr3_4:64989725-64989744 None:intergenic 60.0%
AGCTGCCAGGTGGAAGGTTG+TGG - chr3_4:64989725-64989744 Msa0514300:intron 60.0%
TCGCCTGTGGTGGTAGTAGC+TGG - chr3_4:64989812-64989831 Msa0514300:intron 60.0%
GGGGACGAAGAGAGAGCTGA+TGG + chr3_4:64990091-64990110 None:intergenic 60.0%
GCTCTCTCTTCGTCCCCCTA+TGG - chr3_4:64990094-64990113 Msa0514300:intron 60.0%
!! GGTTTGGGGGAGCTTGCTGT+TGG + chr3_4:64990613-64990632 None:intergenic 60.0%
CGAGAACGAGATACAGGCGC+TGG + chr3_4:64991157-64991176 None:intergenic 60.0%
CGAGGCCACAACCTTCCACC+TGG + chr3_4:64989733-64989752 None:intergenic 65.0%
GTGTATGAGGGAGGGGGGCT+TGG + chr3_4:64991037-64991056 None:intergenic 65.0%
!! AGGGGTGCCGGTTGTCCGAT+TGG + chr3_4:64991061-64991080 None:intergenic 65.0%
!! CGAGATACAGGCGCTGGAGC+TGG + chr3_4:64991151-64991170 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 64987635 64991263 64987635 ID=Msa0514300;Name=Msa0514300
chr3_4 mRNA 64987635 64991263 64987635 ID=Msa0514300-mRNA-1;Parent=Msa0514300;Name=Msa0514300-mRNA-1;_AED=0.12;_eAED=0.12;_QI=1161|0.75|0.8|1|0.75|0.6|5|0|447
chr3_4 exon 64987635 64988002 64987635 ID=Msa0514300-mRNA-1:exon:16491;Parent=Msa0514300-mRNA-1
chr3_4 exon 64988151 64988357 64988151 ID=Msa0514300-mRNA-1:exon:16490;Parent=Msa0514300-mRNA-1
chr3_4 exon 64988783 64988919 64988783 ID=Msa0514300-mRNA-1:exon:16489;Parent=Msa0514300-mRNA-1
chr3_4 exon 64989031 64989707 64989031 ID=Msa0514300-mRNA-1:exon:16488;Parent=Msa0514300-mRNA-1
chr3_4 exon 64990148 64991263 64990148 ID=Msa0514300-mRNA-1:exon:16487;Parent=Msa0514300-mRNA-1
chr3_4 five_prime_UTR 64990148 64991263 64990148 ID=Msa0514300-mRNA-1:five_prime_utr;Parent=Msa0514300-mRNA-1
chr3_4 five_prime_UTR 64989663 64989707 64989663 ID=Msa0514300-mRNA-1:five_prime_utr;Parent=Msa0514300-mRNA-1
chr3_4 CDS 64989031 64989662 64989031 ID=Msa0514300-mRNA-1:cds;Parent=Msa0514300-mRNA-1
chr3_4 CDS 64988783 64988919 64988783 ID=Msa0514300-mRNA-1:cds;Parent=Msa0514300-mRNA-1
chr3_4 CDS 64988151 64988357 64988151 ID=Msa0514300-mRNA-1:cds;Parent=Msa0514300-mRNA-1
chr3_4 CDS 64987635 64988002 64987635 ID=Msa0514300-mRNA-1:cds;Parent=Msa0514300-mRNA-1
Gene Sequence

>Msa0514300

ATGGAGTGGAACGTGAAATCTCCCGGGCAATGGGACTGGGAAAACTTATTCTTCTCGAATTCAAAAGCAGCAGAAACTCACAGGTTACAATCTACTGATTGGAGTATGGAGGAAGATCGAGAAATCAATGTTGGGATGTTGATTCCATCTGGTGGTAGTGGCTATTCAGTGTCTAAACTAATGCATGCTTCATCCTCGAGGAGCTCAAAATCTGCTTCGAATAATTCATCATCAAATGAGGACAGCAAGACATCAATGTTAACTCAAGAAGGTTCTCCAGACAATTCCACCGGTAAGAAAGAATCGTCTAAAGGAGATCCAATTGAAACTTCTCCAGCAGCAGAACCATTGCTCACACTAAAGCTTGGTAAAAGATTCTACTTTGAGGATGTTACCACTGGAAGTCATTCCAAGAAAGCCTCTTCCTCTGCAGTTCCTCTTTTGTGCGGAAAGAAAGGTAAATCGAGCAGTCAGAACATGCTAAATCCAAGCTGCCAGGTGGAAGGTTGTGGCCTCGACCTCTCTTTTGCTAAAGATTACCATCGGAAACATAGAATTTGTGACAGTCATTCCAAATCGCCTGTGGTGGTAGTAGCTGGTTTGGAGCGTCGATTTTGCCAGCAATGTAGCAGGTTCCATGATCTCTCAGAGTTTGATGATAAAAAAAGAAGCTGCAGACGCCGTCTTTCAGATCACAATGCAAGGCGTCGCAAACCTCAGCCTGAAGCAGTGAAATTGAATCCATCAGCTCTCTCTTCGTCCCCCTATGATGGGAGGCAGACAATGGGCCCATTTACTTTTCCAAAGAATACTTCAAATTTAGCATGGCCAGACATGCCCAACAGCAAGCTCCCCCAAACCAAAGATTTTATGCTCAAAACTCCAAAAAACTTCAGCAAGTTTGTCACTATGCTATCTGATGATTCCAGTGGCCACTTTATATCCAAAGGCAAAGGAACCAAGATTGGTGTCCCAGAAACAACTGTTATTCACATTGATAGAATACATATTACAGGTCTAGAAGATCCAAATACCTTGTCTGATCCAAATGCTACACAAGATGTTAACCGTGCTCTCTCTCTTCTGTCAACCAATTCATGGGGTCCATATGATACCAAGCCCCCCTCCCTCATACACTCCAATCGGACAACCGGCACCCCTCAGTATGCTACTGCTCAGCGCTCACCTTTTTCGTCACCGGAATATTGGCACACTGATCAACATCAGGCCAGCTCCAGCGCCTGTATCTCGTTCTCGGGTTACGACAATAGCAATCGCTTTCAAGACTTTCAGCTGTTCAGCGAACCCTATGAGTCAAGTTTCCCTTGCAACCAGCTGGATTAA
Protein sequence

>Msa0514300

MEWNVKSPGQWDWENLFFSNSKAAETHRLQSTDWSMEEDREINVGMLIPSGGSGYSVSKLMHASSSRSSKSASNNSSSNEDSKTSMLTQEGSPDNSTGKKESSKGDPIETSPAAEPLLTLKLGKRFYFEDVTTGSHSKKASSSAVPLLCGKKGKSSSQNMLNPSCQVEGCGLDLSFAKDYHRKHRICDSHSKSPVVVVAGLERRFCQQCSRFHDLSEFDDKKRSCRRRLSDHNARRRKPQPEAVKLNPSALSSSPYDGRQTMGPFTFPKNTSNLAWPDMPNSKLPQTKDFMLKTPKNFSKFVTMLSDDSSGHFISKGKGTKIGVPETTVIHIDRIHITGLEDPNTLSDPNATQDVNRALSLLSTNSWGPYDTKPPSLIHSNRTTGTPQYATAQRSPFSSPEYWHTDQHQASSSACISFSGYDNSNRFQDFQLFSEPYESSFPCNQLD*