Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0517630 | XP_039687084.1 | 81.967 | 122 | 22 | 0 | 1 | 122 | 1 | 122 | 8.01e-69 | 213 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0517630 | G7INX6 | 81.967 | 122 | 22 | 0 | 1 | 122 | 1 | 122 | 3.83e-69 | 213 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0517630 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0517630 | MtrunA17_Chr2g0281511 | 81.967 | 122 | 22 | 0 | 1 | 122 | 1 | 122 | 7.37e-73 | 213 |
| Msa0517630 | MtrunA17_Chr2g0309121 | 79.508 | 122 | 24 | 1 | 1 | 122 | 1 | 121 | 1.46e-68 | 202 |
| Msa0517630 | MtrunA17_Chr3g0081891 | 82.500 | 80 | 14 | 0 | 1 | 80 | 1 | 80 | 6.60e-43 | 135 |
| Msa0517630 | MtrunA17_Chr7g0276661 | 33.871 | 124 | 65 | 4 | 11 | 119 | 45 | 166 | 5.94e-19 | 77.4 |
| Msa0517630 | MtrunA17_Chr5g0430431 | 37.778 | 90 | 46 | 1 | 37 | 116 | 165 | 254 | 1.11e-15 | 70.5 |
| Msa0517630 | MtrunA17_Chr0c01g0489371 | 38.462 | 91 | 54 | 1 | 22 | 110 | 224 | 314 | 1.69e-14 | 68.2 |
| Msa0517630 | MtrunA17_Chr8g0372431 | 28.125 | 96 | 69 | 0 | 24 | 119 | 6 | 101 | 1.29e-13 | 62.0 |
| Msa0517630 | MtrunA17_Chr8g0356961 | 37.079 | 89 | 54 | 1 | 24 | 110 | 77 | 165 | 2.41e-13 | 63.2 |
| Msa0517630 | MtrunA17_Chr7g0228081 | 31.250 | 80 | 55 | 0 | 41 | 120 | 13 | 92 | 4.41e-12 | 57.8 |
| Msa0517630 | MtrunA17_Chr8g0356951 | 35.165 | 91 | 55 | 2 | 24 | 110 | 217 | 307 | 2.44e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.172708 | 3_4:+69330516 | Msa0517630:CDS |
| AAAATGTTGATGTTCATTAT+TGG | 0.262235 | 3_4:+69330488 | Msa0517630:CDS |
| ATTCTCTAATTCAACACTAT+TGG | 0.312782 | 3_4:-69330461 | None:intergenic |
| TGTATGCTACTATCGTTAAC+TGG | 0.336033 | 3_4:+69330877 | Msa0517630:CDS |
| TTTGGTTGAAAATAATGTTT+TGG | 0.336817 | 3_4:+69330534 | Msa0517630:CDS |
| ATCACAGTCATAAGATTTAC+CGG | 0.342234 | 3_4:-69330727 | None:intergenic |
| CGATCAATAATCCTCCTAAT+AGG | 0.349986 | 3_4:+69330853 | Msa0517630:CDS |
| ATAGTAGCATACATCCTATT+AGG | 0.365679 | 3_4:-69330867 | None:intergenic |
| CTTCTTTCTGATGAAGAAAC+CGG | 0.407335 | 3_4:+69330708 | Msa0517630:CDS |
| CTTGAAAAGGGCCTAAAAGT+TGG | 0.503487 | 3_4:+69330813 | Msa0517630:CDS |
| CATCATAAATACATGGGAAG+AGG | 0.531704 | 3_4:+69330774 | Msa0517630:CDS |
| AAGCATGCATCATAAATACA+TGG | 0.533552 | 3_4:+69330767 | Msa0517630:CDS |
| CATTTCTCAGCAACACGTTG+AGG | 0.589472 | 3_4:-69330660 | None:intergenic |
| GTATGCTACTATCGTTAACT+GGG | 0.597139 | 3_4:+69330878 | Msa0517630:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.614063 | 3_4:-69330864 | None:intergenic |
| AGCATGCATCATAAATACAT+GGG | 0.618685 | 3_4:+69330768 | Msa0517630:CDS |
| ATAAATACATGGGAAGAGGA+TGG | 0.641504 | 3_4:+69330778 | Msa0517630:CDS |
| ATGATGCATGCTTCTTGACG+CGG | 0.660170 | 3_4:-69330757 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATGTTGATGTTCATTAT+TGG | + | chr3_4:69330488-69330507 | Msa0517630:CDS | 20.0% |
| !!! | TTTGGTTGAAAATAATGTTT+TGG | + | chr3_4:69330534-69330553 | Msa0517630:CDS | 20.0% |
| !!! | TACAATTTTGTTCTTGAAAA+GGG | + | chr3_4:69330801-69330820 | Msa0517630:CDS | 20.0% |
| ! | ATTCTCTAATTCAACACTAT+TGG | - | chr3_4:69330464-69330483 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | + | chr3_4:69330516-69330535 | Msa0517630:CDS | 25.0% |
| !!! | GAAAATAATGTTTTGGTTAG+TGG | + | chr3_4:69330541-69330560 | Msa0517630:intron | 25.0% |
| !! | GTACAATTTTGTTCTTGAAA+AGG | + | chr3_4:69330800-69330819 | Msa0517630:CDS | 25.0% |
| !! | AAATAACATCACCAACTTTT+AGG | - | chr3_4:69330827-69330846 | None:intergenic | 25.0% |
| ATCACAGTCATAAGATTTAC+CGG | - | chr3_4:69330730-69330749 | None:intergenic | 30.0% | |
| AAGCATGCATCATAAATACA+TGG | + | chr3_4:69330767-69330786 | Msa0517630:CDS | 30.0% | |
| AGCATGCATCATAAATACAT+GGG | + | chr3_4:69330768-69330787 | Msa0517630:CDS | 30.0% | |
| ATAGTAGCATACATCCTATT+AGG | - | chr3_4:69330870-69330889 | None:intergenic | 30.0% | |
| CTTCTTTCTGATGAAGAAAC+CGG | + | chr3_4:69330708-69330727 | Msa0517630:CDS | 35.0% | |
| CATCATAAATACATGGGAAG+AGG | + | chr3_4:69330774-69330793 | Msa0517630:CDS | 35.0% | |
| ATAAATACATGGGAAGAGGA+TGG | + | chr3_4:69330778-69330797 | Msa0517630:CDS | 35.0% | |
| CGATCAATAATCCTCCTAAT+AGG | + | chr3_4:69330853-69330872 | Msa0517630:CDS | 35.0% | |
| TGTATGCTACTATCGTTAAC+TGG | + | chr3_4:69330877-69330896 | Msa0517630:CDS | 35.0% | |
| GTATGCTACTATCGTTAACT+GGG | + | chr3_4:69330878-69330897 | Msa0517630:CDS | 35.0% | |
| CTTGAAAAGGGCCTAAAAGT+TGG | + | chr3_4:69330813-69330832 | Msa0517630:CDS | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | - | chr3_4:69330867-69330886 | None:intergenic | 40.0% | |
| CATTTCTCAGCAACACGTTG+AGG | - | chr3_4:69330663-69330682 | None:intergenic | 45.0% | |
| ATGATGCATGCTTCTTGACG+CGG | - | chr3_4:69330760-69330779 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 69330439 | 69330905 | 69330439 | ID=Msa0517630;Name=Msa0517630 |
| chr3_4 | mRNA | 69330439 | 69330905 | 69330439 | ID=Msa0517630-mRNA-1;Parent=Msa0517630;Name=Msa0517630-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|122 |
| chr3_4 | exon | 69330439 | 69330555 | 69330439 | ID=Msa0517630-mRNA-1:exon:18422;Parent=Msa0517630-mRNA-1 |
| chr3_4 | exon | 69330654 | 69330905 | 69330654 | ID=Msa0517630-mRNA-1:exon:18423;Parent=Msa0517630-mRNA-1 |
| chr3_4 | CDS | 69330439 | 69330555 | 69330439 | ID=Msa0517630-mRNA-1:cds;Parent=Msa0517630-mRNA-1 |
| chr3_4 | CDS | 69330654 | 69330905 | 69330654 | ID=Msa0517630-mRNA-1:cds;Parent=Msa0517630-mRNA-1 |
| Gene Sequence |
| Protein sequence |
