AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0518830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518830 MtrunA17_Chr3g0131181 75.000 100 25 0 1 100 185 284 2.43e-51 168
Msa0518830 MtrunA17_Chr8g0385341 72.000 100 28 0 1 100 186 285 1.76e-47 158
Msa0518830 MtrunA17_Chr1g0170281 68.000 100 32 0 1 100 274 373 7.83e-45 152
Msa0518830 MtrunA17_Chr1g0200601 65.000 100 35 0 1 100 186 285 7.30e-44 148
Msa0518830 MtrunA17_Chr1g0176421 67.000 100 33 0 1 100 317 416 1.05e-43 149
Msa0518830 MtrunA17_Chr5g0448721 64.000 100 36 0 1 100 252 351 1.77e-42 145
Msa0518830 MtrunA17_Chr4g0059951 64.000 100 36 0 1 100 229 328 1.09e-41 143
Msa0518830 MtrunA17_Chr1g0176751 64.000 100 36 0 1 100 280 379 2.66e-40 140
Msa0518830 MtrunA17_Chr7g0242561 61.000 100 39 0 1 100 212 311 6.06e-40 138
Msa0518830 MtrunA17_Chr7g0267771 60.000 100 40 0 1 100 244 343 6.07e-40 137
Msa0518830 MtrunA17_Chr1g0173541 56.000 100 44 0 1 100 208 307 2.24e-39 137
Msa0518830 MtrunA17_Chr7g0257751 57.000 100 43 0 1 100 214 313 3.77e-39 136
Msa0518830 MtrunA17_Chr1g0157131 61.000 100 39 0 1 100 230 329 5.41e-39 135
Msa0518830 MtrunA17_Chr5g0444171 61.000 100 39 0 1 100 248 347 5.50e-39 135
Msa0518830 MtrunA17_Chr5g0444191 61.458 96 37 0 1 96 64 159 1.02e-38 128
Msa0518830 MtrunA17_Chr4g0074811 62.000 100 38 0 1 100 247 346 1.11e-38 135
Msa0518830 MtrunA17_Chr3g0098951 62.000 100 38 0 1 100 240 339 1.71e-38 134
Msa0518830 MtrunA17_Chr0c02g0489761 60.000 100 40 0 1 100 234 333 5.47e-38 133
Msa0518830 MtrunA17_Chr7g0267751 59.000 100 41 0 1 100 291 390 1.15e-37 132
Msa0518830 MtrunA17_Chr8g0387671 58.000 100 42 0 1 100 222 321 2.88e-37 130
Msa0518830 MtrunA17_Chr5g0397101 58.416 101 42 0 1 101 223 323 3.50e-37 130
Msa0518830 MtrunA17_Chr5g0441691 58.000 100 42 0 1 100 224 323 3.52e-37 130
Msa0518830 MtrunA17_Chr4g0033371 54.000 100 46 0 1 100 228 327 3.87e-35 125
Msa0518830 MtrunA17_Chr4g0074791 59.184 98 40 0 3 100 254 351 1.39e-33 121
Msa0518830 MtrunA17_Chr5g0405421 51.020 98 48 0 3 100 259 356 1.61e-31 115
Msa0518830 MtrunA17_Chr1g0203881 57.292 96 41 0 6 101 202 297 2.66e-31 114
Msa0518830 MtrunA17_Chr1g0190131 49.505 101 50 1 1 100 161 261 2.37e-29 106
Msa0518830 MtrunA17_Chr8g0374701 46.000 100 54 0 1 100 288 387 1.03e-27 105
Msa0518830 MtrunA17_Chr4g0045261 46.000 100 54 0 1 100 297 396 2.36e-27 103
Msa0518830 MtrunA17_Chr7g0256001 47.000 100 53 0 1 100 301 400 2.83e-27 103
Msa0518830 MtrunA17_Chr5g0402551 46.000 100 54 0 1 100 294 393 3.54e-27 103
Msa0518830 MtrunA17_Chr3g0127571 46.000 100 54 0 1 100 298 397 8.40e-27 102
Msa0518830 MtrunA17_Chr7g0276111 45.000 100 55 0 1 100 304 403 8.89e-26 99.8
Msa0518830 MtrunA17_Chr3g0132591 43.000 100 57 0 1 100 258 357 5.78e-24 94.4
Msa0518830 MtrunA17_Chr7g0274941 41.414 99 56 1 4 100 173 271 3.89e-19 79.7
Msa0518830 MtrunA17_Chr8g0355131 38.947 95 57 1 6 99 208 302 1.87e-16 73.2
Msa0518830 MtrunA17_Chr4g0001331 42.105 95 49 3 8 100 181 271 1.67e-15 70.5
Msa0518830 MtrunA17_Chr8g0346071 41.176 102 54 3 1 100 170 267 3.49e-15 69.3
Msa0518830 MtrunA17_Chr6g0453681 42.105 95 49 2 8 100 178 268 5.03e-15 68.9
Msa0518830 MtrunA17_Chr4g0008561 38.144 97 57 3 6 100 178 273 5.17e-15 68.9
Msa0518830 MtrunA17_Chr6g0468751 42.105 95 49 2 8 100 178 268 5.62e-15 68.9
Msa0518830 MtrunA17_Chr4g0018201 46.067 89 44 3 6 92 947 1033 7.46e-15 68.6
Msa0518830 MtrunA17_Chr6g0478601 41.935 93 44 3 7 94 301 388 1.10e-14 68.2
Msa0518830 MtrunA17_Chr5g0428981 45.652 92 40 4 6 92 951 1037 1.21e-14 68.2
Msa0518830 MtrunA17_Chr3g0140631 36.458 96 58 2 6 100 192 285 1.25e-14 67.4
Msa0518830 MtrunA17_Chr5g0441011 41.489 94 49 3 8 99 175 264 2.57e-14 67.0
Msa0518830 MtrunA17_Chr2g0333821 44.086 93 46 3 8 98 175 263 3.07e-14 67.0
Msa0518830 MtrunA17_Chr4g0013131 39.785 93 50 3 8 98 173 261 4.53e-14 66.2
Msa0518830 MtrunA17_Chr8g0346081 40.000 100 54 3 1 98 170 265 7.71e-14 65.9
Msa0518830 MtrunA17_Chr2g0304111 37.895 95 53 3 8 100 185 275 7.80e-14 65.9
Msa0518830 MtrunA17_Chr5g0428031 39.785 93 48 3 8 97 169 256 8.22e-14 65.5
Msa0518830 MtrunA17_Chr8g0338741 34.653 101 60 2 1 100 245 340 1.17e-13 65.1
Msa0518830 MtrunA17_Chr1g0150321 39.362 94 51 3 8 99 174 263 1.84e-13 64.7
Msa0518830 MtrunA17_Chr4g0069301 34.783 92 54 3 8 97 188 275 2.12e-13 64.3
Msa0518830 MtrunA17_Chr2g0302431 36.957 92 52 3 8 97 182 269 2.75e-13 64.3
Msa0518830 MtrunA17_Chr1g0166101 41.667 96 50 3 7 100 185 276 3.36e-13 63.9
Msa0518830 MtrunA17_Chr8g0351491 34.375 96 60 2 6 100 171 264 3.84e-13 63.2
Msa0518830 MtrunA17_Chr1g0205421 36.082 97 56 3 6 100 171 263 4.03e-13 63.5
Msa0518830 MtrunA17_Chr7g0244561 35.789 95 55 3 6 98 177 267 5.02e-13 63.5
Msa0518830 MtrunA17_Chr5g0432471 37.500 96 53 4 8 101 173 263 5.39e-13 63.2
Msa0518830 MtrunA17_Chr3g0131061 35.714 98 59 2 7 100 160 257 6.92e-13 62.8
Msa0518830 MtrunA17_Chr3g0132671 36.458 96 53 3 8 100 208 298 7.28e-13 62.8
Msa0518830 MtrunA17_Chr3g0135951 41.250 80 46 1 6 84 301 380 1.16e-12 62.4
Msa0518830 MtrunA17_Chr3g0107011 34.375 96 57 2 8 101 175 266 1.57e-12 62.0
Msa0518830 MtrunA17_Chr5g0426521 37.755 98 57 2 7 100 160 257 2.41e-12 61.2
Msa0518830 MtrunA17_Chr8g0353621 31.250 96 63 2 6 100 165 258 3.39e-12 60.5
Msa0518830 MtrunA17_Chr6g0453671 38.947 95 52 2 8 100 178 268 3.48e-12 60.8
Msa0518830 MtrunA17_Chr2g0284011 35.789 95 55 3 8 100 173 263 3.49e-12 60.8
Msa0518830 MtrunA17_Chr1g0188031 40.449 89 46 3 8 93 183 267 3.73e-12 60.8
Msa0518830 MtrunA17_Chr2g0284031 33.000 100 61 3 3 100 187 282 5.63e-12 60.5
Msa0518830 MtrunA17_Chr7g0260191 36.893 103 51 4 4 100 161 255 6.89e-12 60.1
Msa0518830 MtrunA17_Chr1g0173921 33.333 99 60 3 4 100 161 255 1.00e-11 59.7
Msa0518830 MtrunA17_Chr7g0244521 35.789 95 55 2 8 100 173 263 1.06e-11 59.7
Msa0518830 MtrunA17_Chr1g0183651 40.000 80 47 1 6 84 318 397 1.82e-11 58.9
Msa0518830 MtrunA17_Chr1g0187581 40.909 88 50 2 6 92 316 402 1.93e-11 58.9
Msa0518830 MtrunA17_Chr2g0299781 35.714 98 60 2 6 100 320 417 1.98e-11 58.9
Msa0518830 MtrunA17_Chr4g0025671 39.796 98 55 3 4 100 165 259 1.98e-11 58.9
Msa0518830 MtrunA17_Chr7g0261491 40.000 80 47 1 6 84 318 397 2.45e-11 58.5
Msa0518830 MtrunA17_Chr1g0153321 42.500 80 45 1 6 84 308 387 2.55e-11 58.5
Msa0518830 MtrunA17_Chr7g0258051 34.343 99 59 3 4 100 173 267 3.12e-11 58.2
Msa0518830 MtrunA17_Chr1g0167351 40.000 80 47 1 6 84 310 389 4.16e-11 57.8
Msa0518830 MtrunA17_Chr2g0326531 32.632 95 58 2 8 100 287 377 4.20e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0518830 AT5G04870.1 69.000 100 31 0 1 100 306 405 1.06e-45 155
Msa0518830 AT3G10660.2 68.000 100 32 0 1 100 342 441 5.09e-45 154
Msa0518830 AT3G10660.1 68.000 100 32 0 1 100 342 441 5.09e-45 154
Msa0518830 AT5G23580.1 66.000 100 34 0 1 100 178 277 7.90e-45 151
Msa0518830 AT2G35890.2 68.000 100 32 0 1 100 288 387 1.48e-44 150
Msa0518830 AT2G35890.1 68.000 100 32 0 1 100 288 387 1.48e-44 150
Msa0518830 AT2G38910.1 65.000 100 35 0 1 100 290 389 3.11e-43 148
Msa0518830 AT1G35670.1 67.708 96 31 0 5 100 186 281 3.94e-43 146
Msa0518830 AT2G17290.1 64.000 100 36 0 1 100 241 340 2.36e-42 145
Msa0518830 AT2G17290.2 64.000 100 36 0 1 100 241 340 2.36e-42 145
Msa0518830 AT4G38230.1 63.000 100 37 0 1 100 36 135 2.36e-42 141
Msa0518830 AT1G76040.1 60.000 100 40 0 1 100 30 129 3.92e-42 140
Msa0518830 AT1G76040.4 60.000 100 40 0 1 100 30 129 3.92e-42 140
Msa0518830 AT4G09570.1 65.625 96 33 0 5 100 185 280 5.53e-42 144
Msa0518830 AT4G35310.2 63.000 100 37 0 1 100 253 352 8.23e-42 144
Msa0518830 AT4G35310.1 63.000 100 37 0 1 100 253 352 8.23e-42 144
Msa0518830 AT5G12480.2 60.396 101 40 0 1 101 121 221 1.82e-41 141
Msa0518830 AT4G38230.4 63.000 100 37 0 1 100 180 279 2.31e-41 142
Msa0518830 AT4G38230.3 63.000 100 37 0 1 100 180 279 2.31e-41 142
Msa0518830 AT3G51850.3 60.000 100 40 0 1 100 210 309 3.14e-41 140
Msa0518830 AT4G38230.2 63.000 100 37 0 1 100 210 309 4.44e-41 141
Msa0518830 AT5G12480.1 61.000 100 39 0 1 100 215 314 5.21e-41 141
Msa0518830 AT3G51850.2 60.000 100 40 0 1 100 210 309 9.93e-41 140
Msa0518830 AT4G23650.1 63.000 100 37 0 1 100 234 333 1.77e-40 140
Msa0518830 AT3G51850.1 60.000 100 40 0 1 100 210 309 1.96e-40 140
Msa0518830 AT5G19450.2 59.000 100 41 0 1 100 213 312 1.68e-39 137
Msa0518830 AT5G19450.1 59.000 100 41 0 1 100 213 312 1.68e-39 137
Msa0518830 AT1G76040.3 60.000 100 40 0 1 100 268 367 1.86e-39 137
Msa0518830 AT3G57530.2 58.000 100 42 0 1 100 219 318 2.41e-39 135
Msa0518830 AT1G76040.2 60.000 100 40 0 1 100 268 367 2.44e-39 137
Msa0518830 AT3G57530.1 58.000 100 42 0 1 100 219 318 7.37e-39 135
Msa0518830 AT1G50700.3 58.586 99 41 0 2 100 230 328 1.64e-38 134
Msa0518830 AT2G41860.1 59.000 100 41 0 1 100 210 309 1.73e-38 134
Msa0518830 AT1G50700.1 58.586 99 41 0 2 100 230 328 2.17e-38 134
Msa0518830 AT1G50700.2 58.586 99 41 0 2 100 230 328 2.49e-38 134
Msa0518830 AT1G61950.1 60.000 100 40 0 1 100 255 354 2.98e-38 134
Msa0518830 AT4G04720.2 59.000 100 41 0 1 100 236 335 4.22e-38 134
Msa0518830 AT4G04720.1 59.000 100 41 0 1 100 236 335 4.22e-38 134
Msa0518830 AT2G41860.2 58.000 100 42 0 1 100 210 309 4.78e-38 133
Msa0518830 AT3G20410.2 58.000 100 42 0 1 100 247 346 1.51e-37 132
Msa0518830 AT3G20410.1 58.000 100 42 0 1 100 247 346 1.51e-37 132
Msa0518830 AT1G74740.1 59.000 100 41 0 1 100 215 314 1.68e-37 132
Msa0518830 AT5G12180.1 60.000 100 40 0 1 100 229 328 2.11e-37 132
Msa0518830 AT1G18890.1 57.000 100 43 0 1 100 219 318 2.58e-37 132
Msa0518830 AT4G04740.2 60.204 98 39 0 3 100 227 324 2.82e-37 131
Msa0518830 AT4G04740.4 60.204 98 39 0 3 100 227 324 3.26e-37 131
Msa0518830 AT4G04740.6 60.204 98 39 0 3 100 227 324 3.26e-37 131
Msa0518830 AT4G04740.5 60.204 98 39 0 3 100 227 324 3.26e-37 131
Msa0518830 AT4G04740.3 60.204 98 39 0 3 100 227 324 3.26e-37 131
Msa0518830 AT4G04740.1 60.204 98 39 0 3 100 227 324 3.26e-37 131
Msa0518830 AT4G04740.7 60.204 98 39 0 3 100 227 324 3.26e-37 131
Msa0518830 AT4G21940.3 56.000 100 44 0 1 100 258 357 4.92e-37 130
Msa0518830 AT5G19360.1 60.000 100 40 0 1 100 224 323 5.21e-37 130
Msa0518830 AT4G21940.1 56.000 100 44 0 1 100 258 357 7.38e-37 130
Msa0518830 AT4G21940.2 56.000 100 44 0 1 100 258 357 8.03e-37 130
Msa0518830 AT2G31500.1 55.000 100 45 0 1 100 222 321 1.81e-36 129
Msa0518830 AT5G66210.6 51.515 99 48 0 3 101 222 320 1.15e-31 115
Msa0518830 AT5G66210.4 51.515 99 48 0 3 101 222 320 1.15e-31 115
Msa0518830 AT2G17890.1 51.000 100 49 0 1 100 266 365 1.58e-31 116
Msa0518830 AT5G66210.5 51.515 99 48 0 3 101 222 320 1.69e-31 115
Msa0518830 AT5G66210.3 51.515 99 48 0 3 101 222 320 2.19e-31 115
Msa0518830 AT5G66210.1 51.515 99 48 0 3 101 222 320 2.73e-31 115
Msa0518830 AT5G66210.2 51.515 99 48 0 3 101 222 320 2.73e-31 115
Msa0518830 AT4G04710.2 55.000 100 39 1 1 100 196 289 2.08e-30 112
Msa0518830 AT4G04710.3 55.000 100 39 1 1 100 182 275 2.19e-30 112
Msa0518830 AT4G04710.1 52.336 107 44 1 1 100 196 302 7.33e-30 111
Msa0518830 AT4G04710.4 52.336 107 44 1 1 100 196 302 7.43e-30 111
Msa0518830 AT1G12580.1 52.000 100 48 0 1 100 200 299 9.04e-30 110
Msa0518830 AT1G08650.1 50.495 101 49 1 1 100 170 270 1.66e-29 107
Msa0518830 AT1G08650.2 51.000 100 48 1 1 99 170 269 3.83e-29 105
Msa0518830 AT4G36070.2 47.959 98 51 0 3 100 231 328 6.51e-29 108
Msa0518830 AT4G36070.1 47.475 99 52 0 3 101 231 329 6.51e-29 108
Msa0518830 AT3G04530.1 52.475 101 47 1 1 100 166 266 3.35e-28 103
Msa0518830 AT3G50530.2 46.000 100 54 0 1 100 339 438 2.00e-27 104
Msa0518830 AT3G50530.1 46.000 100 54 0 1 100 308 407 2.30e-27 104
Msa0518830 AT4G04695.1 47.000 100 53 0 1 100 188 287 2.17e-26 101
Msa0518830 AT3G19100.1 46.000 100 54 0 1 100 304 403 2.43e-26 101
Msa0518830 AT2G41140.1 45.000 100 55 0 1 100 283 382 4.63e-26 100
Msa0518830 AT1G12680.1 47.000 100 53 0 1 100 253 352 4.77e-26 100
Msa0518830 AT3G56760.1 44.000 100 56 0 1 100 284 383 1.08e-25 99.8
Msa0518830 AT5G24430.1 45.000 100 55 0 1 100 303 402 2.27e-25 99.0
Msa0518830 AT2G46700.2 44.000 100 56 0 1 100 122 221 4.63e-25 97.4
Msa0518830 AT2G46700.3 44.000 100 56 0 1 100 122 221 4.63e-25 97.4
Msa0518830 AT3G49370.1 46.000 100 54 0 1 100 302 401 5.91e-25 97.8
Msa0518830 AT1G49580.1 43.000 100 57 0 1 100 310 409 7.18e-25 97.4
Msa0518830 AT4G04700.1 44.000 100 56 0 1 100 188 287 9.09e-25 97.1
Msa0518830 AT2G46700.1 44.000 100 56 0 1 100 303 402 1.93e-24 96.3
Msa0518830 AT1G45160.1 43.689 103 45 4 6 100 855 952 5.14e-18 78.2
Msa0518830 AT1G45160.2 43.689 103 45 4 6 100 855 952 5.51e-18 78.2
Msa0518830 AT5G62310.1 45.745 94 41 3 6 94 943 1031 2.06e-16 73.6
Msa0518830 AT1G30270.1 40.000 95 51 3 8 100 193 283 2.51e-16 73.2
Msa0518830 AT1G30270.2 40.000 95 51 3 8 100 193 283 6.12e-16 72.0
Msa0518830 AT1G30270.3 39.175 97 53 3 6 100 94 186 6.20e-16 72.0
Msa0518830 AT3G29160.3 43.158 95 48 2 8 100 179 269 1.17e-15 70.9
Msa0518830 AT4G18700.1 40.000 100 54 3 3 100 182 277 1.25e-15 71.2
Msa0518830 AT3G29160.1 43.158 95 48 2 8 100 179 269 1.26e-15 71.2
Msa0518830 AT3G29160.2 43.158 95 48 2 8 100 179 269 1.26e-15 71.2
Msa0518830 AT5G10930.1 40.426 94 50 3 7 98 173 262 1.74e-15 70.9
Msa0518830 AT5G21326.1 44.086 93 46 3 8 98 175 263 2.25e-15 70.5
Msa0518830 AT3G01090.3 43.158 95 48 2 8 100 178 268 2.56e-15 70.5
Msa0518830 AT3G01090.1 43.158 95 48 2 8 100 178 268 2.56e-15 70.5
Msa0518830 AT3G01090.2 43.158 95 48 2 8 100 201 291 2.81e-15 70.1
Msa0518830 AT5G39440.1 40.426 94 51 2 8 100 178 267 3.00e-15 70.1
Msa0518830 AT5G45810.1 39.796 98 53 3 3 98 184 277 4.72e-15 69.7
Msa0518830 AT4G30960.1 39.785 93 50 3 8 98 185 273 5.50e-15 69.3
Msa0518830 AT5G58380.1 41.304 92 48 3 8 97 173 260 7.38e-15 68.9
Msa0518830 AT2G25880.2 37.500 96 57 2 6 100 148 241 8.04e-15 67.8
Msa0518830 AT2G25880.4 37.500 96 57 2 6 100 193 286 8.13e-15 68.2
Msa0518830 AT2G25880.1 37.500 96 57 2 6 100 180 273 9.34e-15 68.2
Msa0518830 AT2G25880.3 37.500 96 57 2 6 100 241 334 1.20e-14 68.2
Msa0518830 AT5G21222.1 40.426 94 50 3 7 98 174 263 1.49e-14 68.2
Msa0518830 AT5G21222.2 40.426 94 50 3 7 98 174 263 1.49e-14 68.2
Msa0518830 AT5G21222.4 40.426 94 50 3 7 98 174 263 1.49e-14 68.2
Msa0518830 AT5G21222.3 40.426 94 50 3 7 98 174 263 1.49e-14 68.2
Msa0518830 AT2G26980.5 41.935 93 48 3 8 98 176 264 1.97e-14 67.8
Msa0518830 AT2G26980.1 41.935 93 48 3 8 98 176 264 2.49e-14 67.4
Msa0518830 AT2G26980.3 41.935 93 48 3 8 98 176 264 3.09e-14 67.4
Msa0518830 AT2G26980.4 41.935 93 48 3 8 98 186 274 3.30e-14 67.0
Msa0518830 AT1G48260.2 40.206 97 50 3 7 100 87 178 3.54e-14 67.0
Msa0518830 AT1G48260.4 40.206 97 50 3 7 100 87 178 3.54e-14 67.0
Msa0518830 AT1G48260.3 40.206 97 50 3 7 100 87 178 3.54e-14 67.0
Msa0518830 AT1G48260.5 40.206 97 50 3 7 100 87 178 3.54e-14 67.0
Msa0518830 AT1G48260.1 40.625 96 49 3 8 100 173 263 3.66e-14 67.0
Msa0518830 AT4G32830.1 37.500 96 57 2 6 100 186 279 4.12e-14 66.2
Msa0518830 AT2G25090.1 39.130 92 50 3 8 97 185 272 4.68e-14 66.6
Msa0518830 AT5G01810.3 38.710 93 51 3 8 98 173 261 4.76e-14 66.6
Msa0518830 AT5G01810.1 38.710 93 51 3 8 98 173 261 5.32e-14 66.6
Msa0518830 AT5G01810.2 38.710 93 51 3 8 98 173 261 5.32e-14 66.6
Msa0518830 AT5G07070.1 40.860 93 49 3 7 97 172 260 5.44e-14 66.6
Msa0518830 AT4G24400.2 40.860 93 47 3 8 97 169 256 1.12e-13 65.5
Msa0518830 AT3G17850.1 39.362 94 47 3 6 94 1071 1159 1.18e-13 65.9
Msa0518830 AT4G24400.1 40.860 93 47 3 8 97 169 256 1.29e-13 65.5
Msa0518830 AT3G63280.4 37.374 99 56 3 4 100 215 309 1.39e-13 65.5
Msa0518830 AT1G48490.3 41.758 91 49 3 6 94 1011 1099 1.68e-13 65.1
Msa0518830 AT1G48490.2 41.758 91 49 3 6 94 1011 1099 1.68e-13 65.1
Msa0518830 AT1G48490.1 41.758 91 49 3 6 94 1011 1099 1.68e-13 65.1
Msa0518830 AT1G48490.4 41.758 91 49 3 6 94 1012 1100 1.70e-13 65.1
Msa0518830 AT3G63280.2 37.374 99 56 3 4 100 161 255 1.76e-13 65.1
Msa0518830 AT3G63280.3 37.374 99 56 3 4 100 161 255 1.76e-13 65.1
Msa0518830 AT3G63280.1 37.374 99 56 3 4 100 161 255 1.76e-13 65.1
Msa0518830 AT1G01140.2 37.634 93 54 2 8 98 181 271 2.14e-13 64.7
Msa0518830 AT5G25110.1 38.710 93 51 3 8 98 204 292 2.23e-13 64.7
Msa0518830 AT3G61960.2 37.255 102 59 3 1 100 167 265 3.67e-13 64.3
Msa0518830 AT2G34180.1 37.895 95 53 3 8 100 218 308 3.72e-13 64.3
Msa0518830 AT3G61960.1 37.255 102 59 3 1 100 167 265 4.15e-13 63.9
Msa0518830 AT2G26980.2 43.373 83 41 3 8 88 176 254 4.54e-13 63.2
Msa0518830 AT1G29230.1 37.895 95 53 4 8 100 235 325 4.58e-13 63.9
Msa0518830 AT5G09890.1 43.750 80 44 1 6 84 304 383 1.03e-12 62.8
Msa0518830 AT5G09890.2 43.750 80 44 1 6 84 304 383 1.05e-12 62.8
Msa0518830 AT1G78290.3 36.735 98 58 2 7 100 160 257 1.07e-12 62.8
Msa0518830 AT1G78290.2 36.735 98 58 2 7 100 160 257 1.07e-12 62.8
Msa0518830 AT3G53930.2 35.000 100 56 3 6 100 179 274 1.14e-12 62.8
Msa0518830 AT3G53930.1 35.000 100 56 3 6 100 179 274 1.16e-12 62.8
Msa0518830 AT1G30640.3 37.374 99 57 3 6 100 230 327 1.95e-12 62.0
Msa0518830 AT3G17510.2 38.889 90 47 3 8 94 102 186 2.65e-12 61.6
Msa0518830 AT1G30640.2 37.374 99 57 3 6 100 323 420 2.77e-12 61.6
Msa0518830 AT1G30640.1 37.374 99 57 3 6 100 323 420 2.77e-12 61.6
Msa0518830 AT3G17510.1 38.889 90 47 3 8 94 182 266 3.36e-12 61.2
Msa0518830 AT5G45820.1 34.737 95 56 2 8 100 173 263 4.16e-12 61.2
Msa0518830 AT5G35410.1 38.298 94 52 3 1 92 164 253 4.38e-12 61.2
Msa0518830 AT5G35410.2 38.298 94 52 3 1 92 164 253 4.43e-12 60.8
Msa0518830 AT5G57630.3 34.000 100 60 2 1 98 75 170 4.52e-12 60.8
Msa0518830 AT3G25250.1 35.577 104 55 4 1 100 231 326 5.01e-12 60.8
Msa0518830 AT5G57630.1 34.000 100 60 2 1 98 163 258 7.98e-12 60.5
Msa0518830 AT5G57630.2 34.000 100 60 2 1 98 163 258 8.49e-12 60.1
Msa0518830 AT4G33080.1 41.250 80 46 1 6 84 301 380 1.00e-11 60.1
Msa0518830 AT4G33080.2 41.250 80 46 1 6 84 301 380 1.05e-11 60.1
Msa0518830 AT1G03920.2 40.000 80 47 1 6 84 222 301 1.17e-11 59.7
Msa0518830 AT1G03920.1 40.000 80 47 1 6 84 335 414 1.33e-11 59.7
Msa0518830 AT1G03920.3 40.000 80 47 1 6 84 335 414 1.33e-11 59.7
Msa0518830 AT3G20860.1 36.364 99 57 3 4 100 160 254 1.38e-11 59.7
Msa0518830 AT2G32510.1 34.000 100 63 3 4 100 157 256 2.20e-11 58.9
Msa0518830 AT2G20470.2 40.000 80 47 1 6 84 321 400 2.62e-11 58.9
Msa0518830 AT2G20470.1 40.000 80 47 1 6 84 321 400 2.62e-11 58.9
Msa0518830 AT2G20470.3 40.000 80 47 1 6 84 321 400 2.68e-11 58.9
Msa0518830 AT4G14350.3 40.909 88 50 2 6 92 318 404 3.02e-11 58.9
Msa0518830 AT4G14350.2 40.909 88 50 2 6 92 318 404 3.86e-11 58.5
Msa0518830 AT4G14350.1 40.909 88 50 2 6 92 318 404 3.86e-11 58.5
Msa0518830 AT4G14350.4 40.909 88 50 2 6 92 318 404 3.86e-11 58.5
Msa0518830 AT2G19400.1 37.363 91 55 2 6 95 312 401 4.40e-11 58.2
Msa0518830 AT3G44200.2 37.755 98 57 3 4 100 165 259 7.64e-11 57.8
Msa0518830 AT3G44200.1 37.755 98 57 3 4 100 165 259 7.64e-11 57.8
Msa0518830 AT4G13000.1 35.577 104 55 4 1 100 224 319 8.54e-11 57.4

Find 27 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCGGGGTGCCTCCGTTT+TGG 0.249111 3_4:+70729530 Msa0518830:CDS
CTGTTTGTTGATCCCCTCCT+TGG 0.331288 3_4:-70729666 None:intergenic
ACCTCTGGTGCAGTATAGCC+TGG 0.423907 3_4:-70729442 None:intergenic
GCACTCCAAATGTCTGCTTC+AGG 0.426410 3_4:-70729484 None:intergenic
ATGGACCTGAAGCAGACATT+TGG 0.437988 3_4:+70729479 Msa0518830:CDS
GAAACATTTAGTCAAGTTGT+TGG 0.446071 3_4:+70729415 Msa0518830:CDS
AGTCAAGTTGTTGGAACCCC+AGG 0.470655 3_4:+70729424 Msa0518830:CDS
TTGGCACTGTCTGAAATTGA+AGG 0.493086 3_4:-70729728 None:intergenic
GAGGTCTTGAACGGGCATTA+TGG 0.493285 3_4:+70729460 Msa0518830:CDS
TTGAAAGGAATCCGGAGAAA+AGG 0.507068 3_4:+70729771 Msa0518830:CDS
TAATGCCCGTTCAAGACCTC+TGG 0.511246 3_4:-70729457 None:intergenic
GAAGCAGACATTTGGAGTGC+CGG 0.534755 3_4:+70729487 Msa0518830:CDS
CCTCTGGTGCAGTATAGCCT+GGG 0.536066 3_4:-70729441 None:intergenic
CTGCACCAGAGGTCTTGAAC+GGG 0.536546 3_4:+70729452 Msa0518830:CDS
GATAATTTACCTCCCCAAAA+CGG 0.550561 3_4:-70729544 None:intergenic
TTCAATTTCAGACAGTGCCA+AGG 0.554669 3_4:+70729730 Msa0518830:CDS
CTCTGGTGCAGTATAGCCTG+GGG 0.599484 3_4:-70729440 None:intergenic
TTTGTATTTCTTATTAAGCG+GGG 0.602317 3_4:+70729516 Msa0518830:CDS
ACTGCACCAGAGGTCTTGAA+CGG 0.607574 3_4:+70729451 Msa0518830:CDS
TAACCTACAGAGACCAAGGA+GGG 0.610754 3_4:+70729653 Msa0518830:intron
TTGATTTGCAGTCTAAGCCG+TGG 0.614559 3_4:+70729705 Msa0518830:CDS
CTGTCTGAAATTGAAGGCCA+CGG 0.632704 3_4:-70729722 None:intergenic
CCCAGGCTATACTGCACCAG+AGG 0.637073 3_4:+70729441 Msa0518830:CDS
TAAGAAATACAAAATAACGC+CGG 0.669744 3_4:-70729506 None:intergenic
AACCTACAGAGACCAAGGAG+GGG 0.676140 3_4:+70729654 Msa0518830:intron
AATTTACCTCCCCAAAACGG+AGG 0.704130 3_4:-70729541 None:intergenic
TTAACCTACAGAGACCAAGG+AGG 0.760856 3_4:+70729652 Msa0518830:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGTATTTCTTATTAAG+CGG + chr3_4:70729514-70729533 Msa0518830:CDS 15.0%
!!! TTTTGTATTTCTTATTAAGC+GGG + chr3_4:70729515-70729534 Msa0518830:CDS 20.0%
!!! AAATTATCATTAGTTGATGT+AGG + chr3_4:70729559-70729578 Msa0518830:intron 20.0%
! TAAGAAATACAAAATAACGC+CGG - chr3_4:70729509-70729528 None:intergenic 25.0%
! TTTGTATTTCTTATTAAGCG+GGG + chr3_4:70729516-70729535 Msa0518830:CDS 25.0%
! AATAAGATACTTGCTCTATA+AGG - chr3_4:70729833-70729852 None:intergenic 25.0%
! TTTGAATAATTATCACAACG+AGG + chr3_4:70729863-70729882 Msa0518830:intron 25.0%
! GAAACATTTAGTCAAGTTGT+TGG + chr3_4:70729415-70729434 Msa0518830:CDS 30.0%
! ATCAACAAACAGATTTTGCA+AGG + chr3_4:70729677-70729696 Msa0518830:CDS 30.0%
TACTTCGAAAAATGCTTGAA+AGG + chr3_4:70729756-70729775 Msa0518830:CDS 30.0%
AAAAATGCTTGAAAGGAATC+CGG + chr3_4:70729763-70729782 Msa0518830:CDS 30.0%
TTGAAAGTTATAGGCTTATG+TGG + chr3_4:70729886-70729905 Msa0518830:intron 30.0%
GATAATTTACCTCCCCAAAA+CGG - chr3_4:70729547-70729566 None:intergenic 35.0%
CAGCACAATATTAGCATTAC+TGG - chr3_4:70729624-70729643 None:intergenic 35.0%
! TTTTTAACCTACAGAGACCA+AGG + chr3_4:70729649-70729668 Msa0518830:intron 35.0%
! ACAAACAGATTTTGCAAGGA+AGG + chr3_4:70729681-70729700 Msa0518830:CDS 35.0%
!! CATTTTTCGAAGTAGATCCT+TGG - chr3_4:70729750-70729769 None:intergenic 35.0%
ACAACGAGGTTGAAAGTTAT+AGG + chr3_4:70729877-70729896 Msa0518830:intron 35.0%
!!! TAGGCTTATGTGGTTTTTTG+TGG + chr3_4:70729896-70729915 Msa0518830:intron 35.0%
TGAAAACAGTGACATGCCTA+CGG - chr3_4:70729585-70729604 None:intergenic 40.0%
! TTGGCACTGTCTGAAATTGA+AGG - chr3_4:70729731-70729750 None:intergenic 40.0%
TTCAATTTCAGACAGTGCCA+AGG + chr3_4:70729730-70729749 Msa0518830:CDS 40.0%
TTGAAAGGAATCCGGAGAAA+AGG + chr3_4:70729771-70729790 Msa0518830:CDS 40.0%
!!! CTTATGTGGTTTTTTGTGGC+AGG + chr3_4:70729900-70729919 Msa0518830:intron 40.0%
ATGGACCTGAAGCAGACATT+TGG + chr3_4:70729479-70729498 Msa0518830:CDS 45.0%
AATTTACCTCCCCAAAACGG+AGG - chr3_4:70729544-70729563 None:intergenic 45.0%
! CATTAGTTGATGTAGGCCGT+AGG + chr3_4:70729566-70729585 Msa0518830:intron 45.0%
TTAACCTACAGAGACCAAGG+AGG + chr3_4:70729652-70729671 Msa0518830:intron 45.0%
TAACCTACAGAGACCAAGGA+GGG + chr3_4:70729653-70729672 Msa0518830:intron 45.0%
!! TTGATTTGCAGTCTAAGCCG+TGG + chr3_4:70729705-70729724 Msa0518830:CDS 45.0%
! CTGTCTGAAATTGAAGGCCA+CGG - chr3_4:70729725-70729744 None:intergenic 45.0%
! TGAGCTGTGAACCTTTTCTC+CGG - chr3_4:70729785-70729804 None:intergenic 45.0%
! AGTCAAGTTGTTGGAACCCC+AGG + chr3_4:70729424-70729443 Msa0518830:CDS 50.0%
ACTGCACCAGAGGTCTTGAA+CGG + chr3_4:70729451-70729470 Msa0518830:CDS 50.0%
TAATGCCCGTTCAAGACCTC+TGG - chr3_4:70729460-70729479 None:intergenic 50.0%
GAGGTCTTGAACGGGCATTA+TGG + chr3_4:70729460-70729479 Msa0518830:CDS 50.0%
GCACTCCAAATGTCTGCTTC+AGG - chr3_4:70729487-70729506 None:intergenic 50.0%
! GAAGCAGACATTTGGAGTGC+CGG + chr3_4:70729487-70729506 Msa0518830:CDS 50.0%
AACCTACAGAGACCAAGGAG+GGG + chr3_4:70729654-70729673 Msa0518830:intron 50.0%
! CTGTTTGTTGATCCCCTCCT+TGG - chr3_4:70729669-70729688 None:intergenic 50.0%
CTCTGGTGCAGTATAGCCTG+GGG - chr3_4:70729443-70729462 None:intergenic 55.0%
CCTCTGGTGCAGTATAGCCT+GGG - chr3_4:70729444-70729463 None:intergenic 55.0%
ACCTCTGGTGCAGTATAGCC+TGG - chr3_4:70729445-70729464 None:intergenic 55.0%
CTGCACCAGAGGTCTTGAAC+GGG + chr3_4:70729452-70729471 Msa0518830:CDS 55.0%
ATCCCCTCCTTGGTCTCTGT+AGG - chr3_4:70729659-70729678 None:intergenic 55.0%
CCCAGGCTATACTGCACCAG+AGG + chr3_4:70729441-70729460 Msa0518830:CDS 60.0%
!! TAAGCGGGGTGCCTCCGTTT+TGG + chr3_4:70729530-70729549 Msa0518830:CDS 60.0%
!! AAGCGGGGTGCCTCCGTTTT+GGG + chr3_4:70729531-70729550 Msa0518830:CDS 60.0%
!! AGCGGGGTGCCTCCGTTTTG+GGG + chr3_4:70729532-70729551 Msa0518830:CDS 65.0%
!!! GGGGTGCCTCCGTTTTGGGG+AGG + chr3_4:70729535-70729554 Msa0518830:CDS 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 70729415 70729938 70729415 ID=Msa0518830;Name=Msa0518830
chr3_4 mRNA 70729415 70729938 70729415 ID=Msa0518830-mRNA-1;Parent=Msa0518830;Name=Msa0518830-mRNA-1;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.66|1|0.66|3|0|103
chr3_4 exon 70729415 70729556 70729415 ID=Msa0518830-mRNA-1:exon:19309;Parent=Msa0518830-mRNA-1
chr3_4 exon 70729663 70729815 70729663 ID=Msa0518830-mRNA-1:exon:19310;Parent=Msa0518830-mRNA-1
chr3_4 exon 70729922 70729938 70729922 ID=Msa0518830-mRNA-1:exon:19311;Parent=Msa0518830-mRNA-1
chr3_4 CDS 70729415 70729556 70729415 ID=Msa0518830-mRNA-1:cds;Parent=Msa0518830-mRNA-1
chr3_4 CDS 70729663 70729815 70729663 ID=Msa0518830-mRNA-1:cds;Parent=Msa0518830-mRNA-1
chr3_4 CDS 70729922 70729938 70729922 ID=Msa0518830-mRNA-1:cds;Parent=Msa0518830-mRNA-1
Gene Sequence

>Msa0518830

GAAACATTTAGTCAAGTTGTTGGAACCCCAGGCTATACTGCACCAGAGGTCTTGAACGGGCATTATGGACCTGAAGCAGACATTTGGAGTGCCGGCGTTATTTTGTATTTCTTATTAAGCGGGGTGCCTCCGTTTTGGGGAGAGACCAAGGAGGGGATCAACAAACAGATTTTGCAAGGAAGGCTTGATTTGCAGTCTAAGCCGTGGCCTTCAATTTCAGACAGTGCCAAGGATCTACTTCGAAAAATGCTTGAAAGGAATCCGGAGAAAAGGTTCACAGCTCATCAAGTGCTCAGTCATTGTCGACGATAG
Protein sequence

>Msa0518830

ETFSQVVGTPGYTAPEVLNGHYGPEADIWSAGVILYFLLSGVPPFWGETKEGINKQILQGRLDLQSKPWPSISDSAKDLLRKMLERNPEKRFTAHQVLSHCRR*