AlfalfaGEDB | Msa0520120

M. sativa cultivar zhongmu-4 / Msa0520120

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520120	KYP36080.1	64.000	125	41	2	1	121	1	125	3.42e-47	166

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520120	A0A151R0K5	64.000	125	41	2	1	121	1	125	1.64e-47	166

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TFs/TRs:

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Gene ID	Type	Classification
Msa0520120	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520120	MtrunA17_Chr2g0287651	61.468	109	38	1	1	105	117	225	1.13e-41	147
Msa0520120	MtrunA17_Chr1g0203051	38.679	106	61	1	4	105	124	229	1.11e-23	95.9
Msa0520120	MtrunA17_Chr1g0171741	35.294	85	50	2	4	84	178	261	1.88e-11	60.1
Msa0520120	MtrunA17_Chr5g0448761	31.481	108	69	2	2	104	104	211	3.39e-11	60.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0520120	AT1G10240.2	37.000	100	56	2	2	95	93	191	1.49e-11	61.6
Msa0520120	AT1G10240.1	37.000	100	56	2	2	95	93	191	1.49e-11	61.6
Msa0520120	AT4G38180.1	31.776	107	69	2	2	104	117	223	2.98e-11	60.5

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Find 32 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACTTGACTAGGGTCAAT+AGG	0.418818	3_4:-72617686	Msa0520120:CDS
ACATTTATTTCCTGCATACC+GGG	0.472445	3_4:-72617526	Msa0520120:CDS
AGAAAGCTTGAGATGGAGAG+TGG	0.639417	3_4:-72617598	Msa0520120:CDS
AGTTATCAACACCGGATCAG+AGG	0.615238	3_4:+72616537	None:intergenic
ATAATCCAGAAAGCTTGAGA+TGG	0.638128	3_4:-72617605	Msa0520120:intron
ATCAAAACATGTCATATAAT+GGG	0.298640	3_4:-72617453	Msa0520120:CDS
ATGTCATATAATGGGGTACA+TGG	0.556484	3_4:-72617445	Msa0520120:CDS
CAGTTTGTGTGCAATAGAAA+TGG	0.414838	3_4:-72617723	Msa0520120:CDS
CATTTATTTCCTGCATACCG+GGG	0.622384	3_4:-72617525	Msa0520120:CDS
CTGGACAATTATTCCGGGTG+AGG	0.532213	3_4:+72617646	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGCAAAAAACACTTGACTA+GGG	-	chr3_4:72616476-72616495	Msa0520120:CDS	30.0%
TACATTTATTTCCTGCATAC+CGG	-	chr3_4:72616643-72616662	Msa0520120:intron	30.0%
TTTCAAGAAAGCAATGTACT+CGG	-	chr3_4:72617316-72617335	Msa0520120:intron	30.0%
AAAACTCTTCAAATTCCTCT+GGG	+	chr3_4:72617349-72617368	None:intergenic	30.0%
ATACGAGAACAAGGAATTAT+GGG	-	chr3_4:72617424-72617443	Msa0520120:CDS	30.0%
AAGCAAAAAACACTTGACTA+GGG	-	chr3_4:72616476-72616495	Msa0520120:CDS	30.0%
TACATTTATTTCCTGCATAC+CGG	-	chr3_4:72616643-72616662	Msa0520120:intron	30.0%
TTTCAAGAAAGCAATGTACT+CGG	-	chr3_4:72617316-72617335	Msa0520120:intron	30.0%
AAAACTCTTCAAATTCCTCT+GGG	+	chr3_4:72617349-72617368	None:intergenic	30.0%
ATACGAGAACAAGGAATTAT+GGG	-	chr3_4:72617424-72617443	Msa0520120:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	72616441	72617751	72616441	ID=Msa0520120;Name=Msa0520120
chr3_4	mRNA	72616441	72617751	72616441	ID=Msa0520120-mRNA-1;Parent=Msa0520120;Name=Msa0520120-mRNA-1;_AED=0.32;_eAED=0.40;_QI=0\|0\|0\|1\|0\|0\|3\|0\|149
chr3_4	exon	72617631	72617751	72617631	ID=Msa0520120-mRNA-1:exon:20146;Parent=Msa0520120-mRNA-1
chr3_4	exon	72617419	72617618	72617419	ID=Msa0520120-mRNA-1:exon:20145;Parent=Msa0520120-mRNA-1
chr3_4	exon	72616441	72616566	72616441	ID=Msa0520120-mRNA-1:exon:20144;Parent=Msa0520120-mRNA-1
chr3_4	CDS	72617631	72617751	72617631	ID=Msa0520120-mRNA-1:cds;Parent=Msa0520120-mRNA-1
chr3_4	CDS	72617419	72617618	72617419	ID=Msa0520120-mRNA-1:cds;Parent=Msa0520120-mRNA-1
chr3_4	CDS	72616441	72616566	72616441	ID=Msa0520120-mRNA-1:cds;Parent=Msa0520120-mRNA-1

Gene Sequence

>Msa0520120

ATGCGACAGTTTGTGTGCAATAGAAATGGATTAAGAAGCAAAAAACACTTGACTAGGGTCAATAGGAAAAGAGAACATAGACCCCTCACCCGGAATAATTGTCCAGCTCGCCTTCGTATACAAAGCTTGAGATGGAGAGTGGTAATTTTTGAAGGGTGTCATAACCATGAACTAACCCCATCAAAATATATACATTTATTTCCTGCATACCGGGGATTGACGGATGCTGATAAAGCTCAAGTTGACAGTATGCAATCGTATGGTATCAAAACATGTCATATAATGGGGTACATGGTGGCAAACAAGGGTGGATATGATGCTAGTAATACCTCTGATCCGGTGTTGATAACTGGTCTGGATAATATTGAGAAAGATGCTGCAAAGATTTATACATCCGAGATATTCAAAAAAGTTAGAAAACACATTGTCAAGTCTTCTGCGTTAATT

Protein sequence

>Msa0520120

MRQFVCNRNGLRSKKHLTRVNRKREHRPLTRNNCPARLRIQSLRWRVVIFEGCHNHELTPSKYIHLFPAYRGLTDADKAQVDSMQSYGIKTCHIMGYMVANKGGYDASNTSDPVLITGLDNIEKDAAKIYTSEIFKKVRKHIVKSSALI