AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0523890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523890 MtrunA17_Chr3g0145041 91.489 188 16 0 5 192 9 196 4.42e-131 365
Msa0523890 MtrunA17_Chr3g0145031 70.619 194 53 1 3 192 25 218 9.66e-103 295
Msa0523890 MtrunA17_Chr2g0316511 55.155 194 83 2 3 192 23 216 3.66e-76 227
Msa0523890 MtrunA17_Chr2g0324381 50.292 171 79 3 3 168 30 199 3.37e-56 176
Msa0523890 MtrunA17_Chr5g0447451 44.361 133 64 4 44 166 11 143 4.41e-29 105
Msa0523890 MtrunA17_Chr2g0324361 48.980 98 44 3 6 100 13 107 5.02e-22 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523890 AT4G39100.1 59.162 191 73 2 3 189 23 212 8.80e-85 250
Msa0523890 AT4G22140.2 56.186 194 81 2 3 192 31 224 1.87e-81 241
Msa0523890 AT4G22140.1 55.615 187 79 2 3 185 31 217 5.29e-77 230
Msa0523890 AT4G22140.3 55.615 187 79 2 3 185 31 217 5.29e-77 230
Msa0523890 AT4G39100.2 63.121 141 48 1 3 139 23 163 2.13e-66 201
Msa0523890 AT4G04260.2 47.619 189 93 3 6 189 9 196 2.85e-59 184
Msa0523890 AT4G04260.1 46.739 184 92 3 11 189 1 183 3.79e-56 176
Msa0523890 AT4G04260.3 55.085 118 50 1 2 116 23 140 3.70e-41 136

Find 59 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGAATACCTTTGTATT+TGG 0.149988 3_4:+77334621 None:intergenic
AATGAAGTTGTCAATTCTTT+CGG 0.316759 3_4:+77334707 None:intergenic
TTAGGAATACCTTTGTATTT+GGG 0.355673 3_4:+77334622 None:intergenic
AAGGACCCGATGAGGATATA+TGG 0.371664 3_4:+77333575 None:intergenic
ACCATCACTTTCATCTTTGA+CGG 0.371838 3_4:+77333774 None:intergenic
AAAATGCACTGTTCACAGTT+TGG 0.374580 3_4:-77336342 Msa0523890:CDS
CACTGGATCATAACTTCATC+AGG 0.392678 3_4:+77336032 None:intergenic
CATAACTTCATCAGGGTTGT+AGG 0.393557 3_4:+77336040 None:intergenic
TTCGTGTCCGTTGGTACTAC+TGG 0.395721 3_4:-77336463 Msa0523890:CDS
GAAAGTCTTAATGTGTATAC+TGG 0.395769 3_4:+77333503 None:intergenic
ACTCACACTTGAACTATATC+AGG 0.399463 3_4:+77336236 None:intergenic
TGTTGGTATCCACGGTTTAG+AGG 0.405406 3_4:+77336529 None:intergenic
GGTGTACATTAGATATATAA+TGG 0.440667 3_4:-77333754 Msa0523890:three_prime_UTR
TTGAACTACAATTCCGCTAC+TGG 0.456893 3_4:-77336269 Msa0523890:CDS
ATTCTCCATATATCCTCATC+GGG 0.523816 3_4:-77333580 Msa0523890:three_prime_UTR
ATCACTGTTTCCCTAGGTAG+TGG 0.526395 3_4:-77333646 Msa0523890:three_prime_UTR
CGACCCTTCTTTGTCTCCTC+CGG 0.527955 3_4:+77336440 None:intergenic
TACATGAAGCTTGATGTTGT+TGG 0.528797 3_4:-77336314 Msa0523890:CDS
GATTCTCCATATATCCTCAT+CGG 0.530891 3_4:-77333581 Msa0523890:three_prime_UTR
AGTGTGATCGCTGCACTGAT+TGG 0.536011 3_4:-77336013 Msa0523890:intron
GCCACATATGTTGGTATCCA+CGG 0.537762 3_4:+77336521 None:intergenic
AAACACATCCTCCACTACCT+AGG 0.542451 3_4:+77333635 None:intergenic
CAACTGCCAACACGATTCAC+TGG 0.547820 3_4:+77333474 None:intergenic
CAAGAGATGTTCGTGTCCGT+TGG 0.548163 3_4:-77336472 Msa0523890:CDS
TACATTAGATATATAATGGA+CGG 0.550351 3_4:-77333750 Msa0523890:three_prime_UTR
ACCAAGGATTTGAAAAGCTA+GGG 0.551461 3_4:+77333725 None:intergenic
ATAACTTCATCAGGGTTGTA+GGG 0.552648 3_4:+77336041 None:intergenic
TACCAAGGATTTGAAAAGCT+AGG 0.554541 3_4:+77333724 None:intergenic
AGTTTATTCAGCTCCTACCA+AGG 0.567083 3_4:+77333709 None:intergenic
CAGTGAATCGTGTTGGCAGT+TGG 0.569218 3_4:-77333473 Msa0523890:three_prime_UTR
AACACATCCTCCACTACCTA+GGG 0.569275 3_4:+77333636 None:intergenic
ACCGTCATGTCTATACAAGC+AGG 0.569799 3_4:+77334738 None:intergenic
AAGGGTCGGCGTCATTTCCA+TGG 0.570085 3_4:-77336425 Msa0523890:CDS
CTGGCCGGAGGAGACAAAGA+AGG 0.572097 3_4:-77336444 Msa0523890:CDS
ACCGTGGATACCAACATATG+TGG 0.579459 3_4:-77336522 Msa0523890:CDS
TGTCCGTTGGTACTACTGGC+CGG 0.580901 3_4:-77336459 Msa0523890:CDS
AGCTGAATAAACTCTGACAA+TGG 0.581923 3_4:-77333699 Msa0523890:three_prime_UTR
TCGATCATAGCCACATATGT+TGG 0.588140 3_4:+77336512 None:intergenic
ACATTAGATATATAATGGAC+GGG 0.589985 3_4:-77333749 Msa0523890:three_prime_UTR
GTAACCATCACTGTTTCCCT+AGG 0.592283 3_4:-77333652 Msa0523890:three_prime_UTR
GCGTCATTTCCATGGCTCAA+AGG 0.593809 3_4:-77336417 Msa0523890:CDS
CAAAAGTCTGAGTATGAACA+AGG 0.609731 3_4:+77333556 None:intergenic
TATTCACCAGTGAATCGTGT+TGG 0.610674 3_4:-77333480 Msa0523890:three_prime_UTR
CGGAGGAGACAAAGAAGGGT+CGG 0.615656 3_4:-77336439 Msa0523890:CDS
TCCTGCTTGTATAGACATGA+CGG 0.620123 3_4:-77334739 Msa0523890:CDS
ACTACCTAGGGAAACAGTGA+TGG 0.626362 3_4:+77333648 None:intergenic
AATCAGTGCAGCGATCACAC+TGG 0.630754 3_4:+77336015 None:intergenic
ACTGTTTCCCTAGGTAGTGG+AGG 0.634735 3_4:-77333643 Msa0523890:three_prime_UTR
TGGCCGGAGGAGACAAAGAA+GGG 0.641254 3_4:-77336443 Msa0523890:CDS
CAGAGTGTTGATACAATCGA+AGG 0.642499 3_4:-77336365 Msa0523890:CDS
AGGAGTGAGAGCACCAGTAG+CGG 0.642633 3_4:+77336256 None:intergenic
GCCGTCAAAGATGAAAGTGA+TGG 0.650227 3_4:-77333775 Msa0523890:exon
CCTCCGGCCAGTAGTACCAA+CGG 0.658998 3_4:+77336456 None:intergenic
TGCAAGACTCCCAAATACAA+AGG 0.662219 3_4:-77334631 Msa0523890:intron
ACTGGATCATAACTTCATCA+GGG 0.673831 3_4:+77336033 None:intergenic
GTATGAACAAGGACCCGATG+AGG 0.693701 3_4:+77333567 None:intergenic
GCATGCGTTCCTCTAAACCG+TGG 0.713047 3_4:-77336538 Msa0523890:CDS
CCGTTGGTACTACTGGCCGG+AGG 0.722355 3_4:-77336456 Msa0523890:CDS
TGCTTGTATAGACATGACGG+TGG 0.750089 3_4:-77334736 Msa0523890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGGTTATTATTTAATTAA+GGG + chr3_4:77334909-77334928 None:intergenic 10.0%
!!! AAATTTTTCTTCTAAATAAA+AGG - chr3_4:77336184-77336203 Msa0523890:intron 10.0%
!! AATAATAGGAAAATATGTAA+AGG + chr3_4:77333836-77333855 None:intergenic 15.0%
!! ATTGTAAAAAATAAAAGGAA+AGG - chr3_4:77334370-77334389 Msa0523890:intron 15.0%
!! ATAATTGAAATTCATTTGAA+CGG - chr3_4:77334561-77334580 Msa0523890:intron 15.0%
!!! ATTTTTTTTGACAAAATACA+CGG - chr3_4:77334691-77334710 Msa0523890:CDS 15.0%
!! CAAATATAGAAAAAACTTAT+TGG - chr3_4:77334766-77334785 Msa0523890:intron 15.0%
!! CTATGGTTATTATTTAATTA+AGG + chr3_4:77334910-77334929 None:intergenic 15.0%
!!! GCAAATAAACTAATTTTTTA+TGG + chr3_4:77335149-77335168 None:intergenic 15.0%
!! TATTCTTATTCAAAATACAA+AGG + chr3_4:77336013-77336032 None:intergenic 15.0%
!! TATTTAGAAGAAAAATTTAC+AGG + chr3_4:77336182-77336201 None:intergenic 15.0%
!!! ATTGATGTTTATATTTTGTT+TGG - chr3_4:77336219-77336238 Msa0523890:intron 15.0%
!!! TTCATTGCTCTAAAATTTAA+TGG - chr3_4:77333952-77333971 Msa0523890:intron 20.0%
!!! CATTTCAATGATGTTTTATT+TGG - chr3_4:77334423-77334442 Msa0523890:intron 20.0%
!! TAATTGAAATTCATTTGAAC+GGG - chr3_4:77334562-77334581 Msa0523890:intron 20.0%
!!! AAAATACACGGATTAATTAA+AGG - chr3_4:77334703-77334722 Msa0523890:CDS 20.0%
!!! AAATACACGGATTAATTAAA+GGG - chr3_4:77334704-77334723 Msa0523890:CDS 20.0%
!! AGATTACATTCACATTATAA+AGG + chr3_4:77335250-77335269 None:intergenic 20.0%
!! ATTAACTCAATCATACAAAA+CGG + chr3_4:77335987-77336006 None:intergenic 20.0%
!!! TATATTTTGTTTGGTATTGT+AGG - chr3_4:77336228-77336247 Msa0523890:intron 20.0%
!!! TACATTAGATATATAATGGA+CGG - chr3_4:77336288-77336307 Msa0523890:CDS 20.0%
!! AACTTAGAGAGTGTAATAAT+AGG + chr3_4:77333850-77333869 None:intergenic 25.0%
!!! CTTTTTTGTTACAATGAAGA+AGG - chr3_4:77333893-77333912 Msa0523890:intron 25.0%
! TTTCTGTGTAATTATTTCAG+TGG - chr3_4:77334632-77334651 Msa0523890:CDS 25.0%
!! TAGAGAATCTTTCTTCTTTT+AGG - chr3_4:77334845-77334864 Msa0523890:intron 25.0%
!! TTAGTTTATTTGCTAAACCA+TGG - chr3_4:77335156-77335175 Msa0523890:intron 25.0%
!! TATGTAAATCCTTTTCAAAC+AGG + chr3_4:77335210-77335229 None:intergenic 25.0%
! AATGAAGTTGTCAATTCTTT+CGG + chr3_4:77335334-77335353 None:intergenic 25.0%
!!! TTGCAACTTTTTTTGAACTT+CGG + chr3_4:77335373-77335392 None:intergenic 25.0%
! TTAGGAATACCTTTGTATTT+GGG + chr3_4:77335419-77335438 None:intergenic 25.0%
! CTGATACCATCTTAAAATTT+GGG + chr3_4:77335784-77335803 None:intergenic 25.0%
! TCTGATACCATCTTAAAATT+TGG + chr3_4:77335785-77335804 None:intergenic 25.0%
! TTAACTCAATCATACAAAAC+GGG + chr3_4:77335986-77336005 None:intergenic 25.0%
! TCTATTTCACATAAGTCATT+AGG + chr3_4:77336149-77336168 None:intergenic 25.0%
! GGTGTACATTAGATATATAA+TGG - chr3_4:77336284-77336303 Msa0523890:CDS 25.0%
!!! ACATTAGATATATAATGGAC+GGG - chr3_4:77336289-77336308 Msa0523890:CDS 25.0%
!!! ATGGTTTGAAATTTTGTCAA+TGG - chr3_4:77336358-77336377 Msa0523890:CDS 25.0%
!!! TTTGAAATTTTGTCAATGGT+TGG - chr3_4:77336362-77336381 Msa0523890:CDS 25.0%
TTGTTTGAATTTCAGACTTC+TGG - chr3_4:77334108-77334127 Msa0523890:intron 30.0%
TTGTTCATGACATGTGATAT+AGG - chr3_4:77334175-77334194 Msa0523890:intron 30.0%
! AGACAACAATACTTCTAGTT+AGG - chr3_4:77334206-77334225 Msa0523890:intron 30.0%
!!! TGAGGGGTGTTTTTTTTTTT+GGG + chr3_4:77335034-77335053 None:intergenic 30.0%
!!! TTGAGGGGTGTTTTTTTTTT+TGG + chr3_4:77335035-77335054 None:intergenic 30.0%
! ATTTGCTAAACCATGGTAAA+TGG - chr3_4:77335163-77335182 Msa0523890:intron 30.0%
TTTGCTAAACCATGGTAAAT+GGG - chr3_4:77335164-77335183 Msa0523890:intron 30.0%
CAAATATTTCCCATTTACCA+TGG + chr3_4:77335176-77335195 None:intergenic 30.0%
TGGTAAATGGGAAATATTTG+AGG - chr3_4:77335176-77335195 Msa0523890:intron 30.0%
!! GTTTTAGTACCTGTTTGAAA+AGG - chr3_4:77335198-77335217 Msa0523890:intron 30.0%
CAGCATAATATTTGTGATTC+AGG - chr3_4:77335271-77335290 Msa0523890:intron 30.0%
GTTAGGAATACCTTTGTATT+TGG + chr3_4:77335420-77335439 None:intergenic 30.0%
CTAAAACTGTCAAACAATAG+TGG + chr3_4:77335442-77335461 None:intergenic 30.0%
!! GGTATTAAGTGTTGAAAACA+AGG + chr3_4:77335470-77335489 None:intergenic 30.0%
ACCATTTGAATTTACTTACC+TGG - chr3_4:77335487-77335506 Msa0523890:intron 30.0%
GATGAAGATATCAAAGATCT+CGG + chr3_4:77335516-77335535 None:intergenic 30.0%
TAATTCCTATTCCAATGTGT+TGG - chr3_4:77335554-77335573 Msa0523890:intron 30.0%
AATTTCCAACACATTGGAAT+AGG + chr3_4:77335562-77335581 None:intergenic 30.0%
AAACAGAATTTCCAACACAT+TGG + chr3_4:77335568-77335587 None:intergenic 30.0%
! ACCATCTTAAAATTTGGGTT+GGG + chr3_4:77335779-77335798 None:intergenic 30.0%
TACCATCTTAAAATTTGGGT+TGG + chr3_4:77335780-77335799 None:intergenic 30.0%
! CATAACACTTCAGGTTTATT+GGG + chr3_4:77335876-77335895 None:intergenic 30.0%
ATATATCCATATTTCTCAGC+TGG + chr3_4:77336051-77336070 None:intergenic 30.0%
GCTGAGAAATATGGATATAT+CGG - chr3_4:77336051-77336070 Msa0523890:CDS 30.0%
TATAAGATTGTGTCTTTCCA+TGG + chr3_4:77336098-77336117 None:intergenic 30.0%
!! ATTTTGTTTGGTATTGTAGG+TGG - chr3_4:77336231-77336250 Msa0523890:intron 30.0%
GAAAGTCTTAATGTGTATAC+TGG + chr3_4:77336538-77336557 None:intergenic 30.0%
AAAATGCACTGTTCACAGTT+TGG - chr3_4:77333696-77333715 Msa0523890:three_prime_UTR 35.0%
TACATGAAGCTTGATGTTGT+TGG - chr3_4:77333724-77333743 Msa0523890:three_prime_UTR 35.0%
ACTCACACTTGAACTATATC+AGG + chr3_4:77333805-77333824 None:intergenic 35.0%
TTGTTACAATGAAGAAGGTG+TGG - chr3_4:77333898-77333917 Msa0523890:intron 35.0%
! TGTTACAATGAAGAAGGTGT+GGG - chr3_4:77333899-77333918 Msa0523890:intron 35.0%
ATAACTTCATCAGGGTTGTA+GGG + chr3_4:77334000-77334019 None:intergenic 35.0%
ACTGGATCATAACTTCATCA+GGG + chr3_4:77334008-77334027 None:intergenic 35.0%
GAACTGTATAAGCAGAACAA+GGG + chr3_4:77334063-77334082 None:intergenic 35.0%
AGAACTGTATAAGCAGAACA+AGG + chr3_4:77334064-77334083 None:intergenic 35.0%
! CAACAATACTTCTAGTTAGG+TGG - chr3_4:77334209-77334228 Msa0523890:intron 35.0%
ATATGTCGTGCTAAAGTTCA+TGG - chr3_4:77334250-77334269 Msa0523890:intron 35.0%
GAATCAGCAGATTGTACAAT+TGG - chr3_4:77334285-77334304 Msa0523890:intron 35.0%
CTTAGAGCTAAGGAAGAAAT+TGG + chr3_4:77334597-77334616 None:intergenic 35.0%
!! CAATTTCTTCCTTAGCTCTA+AGG - chr3_4:77334595-77334614 Msa0523890:intron 35.0%
!! ATCATTAACTTGTGCCTTGA+GGG + chr3_4:77334745-77334764 None:intergenic 35.0%
!! AATCATTAACTTGTGCCTTG+AGG + chr3_4:77334746-77334765 None:intergenic 35.0%
!! AGTGTTAACTTGTGTCCTTA+GGG + chr3_4:77334885-77334904 None:intergenic 35.0%
!! TAGTGTTAACTTGTGTCCTT+AGG + chr3_4:77334886-77334905 None:intergenic 35.0%
!! TTTGGTGGGTTATCTATCTA+TGG + chr3_4:77334927-77334946 None:intergenic 35.0%
!! TTTTTAGGGGTGATGAGTTT+TGG + chr3_4:77334945-77334964 None:intergenic 35.0%
!! ACAATGCTTGCTTTTGTGAA+GGG + chr3_4:77334973-77334992 None:intergenic 35.0%
!! AACAATGCTTGCTTTTGTGA+AGG + chr3_4:77334974-77334993 None:intergenic 35.0%
!!! GAGGGGTGTTTTTTTTTTTG+GGG + chr3_4:77335033-77335052 None:intergenic 35.0%
! TATGGTTTGTGAAGATGTGT+GGG + chr3_4:77335131-77335150 None:intergenic 35.0%
! TTATGGTTTGTGAAGATGTG+TGG + chr3_4:77335132-77335151 None:intergenic 35.0%
ACTGTCAAACAATAGTGGTT+AGG + chr3_4:77335437-77335456 None:intergenic 35.0%
GCCAGGTAAGTAAATTCAAA+TGG + chr3_4:77335491-77335510 None:intergenic 35.0%
TGGCATATGCAGTGTTTATA+AGG - chr3_4:77335709-77335728 Msa0523890:intron 35.0%
TATGCAGTGTTTATAAGGCT+TGG - chr3_4:77335714-77335733 Msa0523890:intron 35.0%
ATGCAGTGTTTATAAGGCTT+GGG - chr3_4:77335715-77335734 Msa0523890:intron 35.0%
!!! GTTACCTTACTGGTTTTGTT+AGG - chr3_4:77335743-77335762 Msa0523890:intron 35.0%
TCAACCTAACAAAACCAGTA+AGG + chr3_4:77335750-77335769 None:intergenic 35.0%
AGCCTATCTTATATCTGTTG+TGG - chr3_4:77335804-77335823 Msa0523890:intron 35.0%
AGCCACAACAGATATAAGAT+AGG + chr3_4:77335809-77335828 None:intergenic 35.0%
AGGTTTATTGGGATAACACT+CGG + chr3_4:77335865-77335884 None:intergenic 35.0%
! CCATAACACTTCAGGTTTAT+TGG + chr3_4:77335877-77335896 None:intergenic 35.0%
! CCAATAAACCTGAAGTGTTA+TGG - chr3_4:77335874-77335893 Msa0523890:intron 35.0%
! ATAAACCTGAAGTGTTATGG+TGG - chr3_4:77335877-77335896 Msa0523890:intron 35.0%
GCAACACAAATGCCTTAATT+AGG + chr3_4:77335936-77335955 None:intergenic 35.0%
ACCATCACTTTCATCTTTGA+CGG + chr3_4:77336267-77336286 None:intergenic 35.0%
ACCAAGGATTTGAAAAGCTA+GGG + chr3_4:77336316-77336335 None:intergenic 35.0%
TACCAAGGATTTGAAAAGCT+AGG + chr3_4:77336317-77336336 None:intergenic 35.0%
! TAGCTTTTCAAATCCTTGGT+AGG - chr3_4:77336316-77336335 Msa0523890:CDS 35.0%
AGCTGAATAAACTCTGACAA+TGG - chr3_4:77336339-77336358 Msa0523890:CDS 35.0%
GATTCTCCATATATCCTCAT+CGG - chr3_4:77336457-77336476 Msa0523890:CDS 35.0%
ATTCTCCATATATCCTCATC+GGG - chr3_4:77336458-77336477 Msa0523890:CDS 35.0%
CAAAAGTCTGAGTATGAACA+AGG + chr3_4:77336485-77336504 None:intergenic 35.0%
TCGATCATAGCCACATATGT+TGG + chr3_4:77333529-77333548 None:intergenic 40.0%
!! CAGAGTGTTGATACAATCGA+AGG - chr3_4:77333673-77333692 Msa0523890:three_prime_UTR 40.0%
TTGAACTACAATTCCGCTAC+TGG - chr3_4:77333769-77333788 Msa0523890:exon 40.0%
CATAACTTCATCAGGGTTGT+AGG + chr3_4:77334001-77334020 None:intergenic 40.0%
CACTGGATCATAACTTCATC+AGG + chr3_4:77334009-77334028 None:intergenic 40.0%
AGAAGTAATGAGACACCAGA+CGG + chr3_4:77334497-77334516 None:intergenic 40.0%
CTCAAGTAGTAGCACATACA+CGG - chr3_4:77334658-77334677 Msa0523890:CDS 40.0%
!! AATGTTAACTTGTGCCCTCA+AGG - chr3_4:77334728-77334747 Msa0523890:CDS 40.0%
GTTTCTTAACATGTGCCCTA+AGG - chr3_4:77334867-77334886 Msa0523890:intron 40.0%
!!! TGTGAAGGGGTCTGTTTTTA+GGG + chr3_4:77334959-77334978 None:intergenic 40.0%
!! TTGTGAAGGGGTCTGTTTTT+AGG + chr3_4:77334960-77334979 None:intergenic 40.0%
!! CAATGCTTGCTTTTGTGAAG+GGG + chr3_4:77334972-77334991 None:intergenic 40.0%
ACCCCTCAAAATAAAACACC+CGG - chr3_4:77335046-77335065 Msa0523890:intron 40.0%
!!! ACCGGGTGTTTTATTTTGAG+GGG + chr3_4:77335050-77335069 None:intergenic 40.0%
!!! GACCGGGTGTTTTATTTTGA+GGG + chr3_4:77335051-77335070 None:intergenic 40.0%
TCCTTAGGATAAGTGCTCTT+CGG + chr3_4:77335097-77335116 None:intergenic 40.0%
TCCTGCTTGTATAGACATGA+CGG - chr3_4:77335299-77335318 Msa0523890:intron 40.0%
TGCAAGACTCCCAAATACAA+AGG - chr3_4:77335407-77335426 Msa0523890:intron 40.0%
GAAGATATCAAAGATCTCGG+AGG + chr3_4:77335513-77335532 None:intergenic 40.0%
!! GCCCAACCCAAATTTTAAGA+TGG - chr3_4:77335775-77335794 Msa0523890:intron 40.0%
GTCTACACTTCAGGCTTATT+GGG - chr3_4:77335838-77335857 Msa0523890:intron 40.0%
ACTTTCCACCATAACACTTC+AGG + chr3_4:77335885-77335904 None:intergenic 40.0%
ATGCCTTAATTAGGCAGCTA+GGG + chr3_4:77335927-77335946 None:intergenic 40.0%
AATGCCTTAATTAGGCAGCT+AGG + chr3_4:77335928-77335947 None:intergenic 40.0%
! ATCATACAAAACGGGCTTGA+GGG + chr3_4:77335978-77335997 None:intergenic 40.0%
! AATCATACAAAACGGGCTTG+AGG + chr3_4:77335979-77335998 None:intergenic 40.0%
GTTTGACCAGCTGAGAAATA+TGG - chr3_4:77336042-77336061 Msa0523890:CDS 40.0%
ATATCGGCATGCTTCTCTAT+AGG - chr3_4:77336067-77336086 Msa0523890:intron 40.0%
!! ACCCTAGCTTTTCAAATCCT+TGG - chr3_4:77336312-77336331 Msa0523890:CDS 40.0%
AGTTTATTCAGCTCCTACCA+AGG + chr3_4:77336332-77336351 None:intergenic 40.0%
TATTCACCAGTGAATCGTGT+TGG - chr3_4:77336558-77336577 Msa0523890:CDS 40.0%
TGTTGGTATCCACGGTTTAG+AGG + chr3_4:77333512-77333531 None:intergenic 45.0%
ACCGTGGATACCAACATATG+TGG - chr3_4:77333516-77333535 Msa0523890:three_prime_UTR 45.0%
GCCACATATGTTGGTATCCA+CGG + chr3_4:77333520-77333539 None:intergenic 45.0%
GAAAAAGCTCCTTTGAGCCA+TGG + chr3_4:77333633-77333652 None:intergenic 45.0%
GAAGTAATGAGACACCAGAC+GGG + chr3_4:77334496-77334515 None:intergenic 45.0%
!! TTCCTTAGCTCTAAGGTCCT+GGG - chr3_4:77334602-77334621 Msa0523890:intron 45.0%
TACACAGAAATTCTCCTCCC+AGG + chr3_4:77334622-77334641 None:intergenic 45.0%
!! TAGGGGTGATGAGTTTTGGT+GGG + chr3_4:77334941-77334960 None:intergenic 45.0%
!! TTAGGGGTGATGAGTTTTGG+TGG + chr3_4:77334942-77334961 None:intergenic 45.0%
!!! GTGAAGGGGTCTGTTTTTAG+GGG + chr3_4:77334958-77334977 None:intergenic 45.0%
! GTAGTGACGATGAGTGGTTT+TGG + chr3_4:77335007-77335026 None:intergenic 45.0%
!!! GGACCGGGTGTTTTATTTTG+AGG + chr3_4:77335052-77335071 None:intergenic 45.0%
!!! TCAGGTTTGGTTTTAGGACC+GGG + chr3_4:77335067-77335086 None:intergenic 45.0%
!!! CTCAGGTTTGGTTTTAGGAC+CGG + chr3_4:77335068-77335087 None:intergenic 45.0%
!!! TGTGGCTCAGGTTTGGTTTT+AGG + chr3_4:77335073-77335092 None:intergenic 45.0%
!! TCCGAAGAGCACTTATCCTA+AGG - chr3_4:77335093-77335112 Msa0523890:intron 45.0%
ACCGTCATGTCTATACAAGC+AGG + chr3_4:77335303-77335322 None:intergenic 45.0%
TGCTTGTATAGACATGACGG+TGG - chr3_4:77335302-77335321 Msa0523890:intron 45.0%
AGGATGCAATGAAGACAACC+CGG + chr3_4:77335628-77335647 None:intergenic 45.0%
TCCAACACCTTCGTTGTGTT+GGG - chr3_4:77335647-77335666 Msa0523890:intron 45.0%
TGATTTAGTCCCACTTTGCC+TGG - chr3_4:77335681-77335700 Msa0523890:intron 45.0%
ACTGCATATGCCATATGTCC+AGG + chr3_4:77335702-77335721 None:intergenic 45.0%
TTGGGTAGCAGTTACCTTAC+TGG - chr3_4:77335733-77335752 Msa0523890:intron 45.0%
TTTCGCATCGTCTACACTTC+AGG - chr3_4:77335829-77335848 Msa0523890:intron 45.0%
CGTCTACACTTCAGGCTTAT+TGG - chr3_4:77335837-77335856 Msa0523890:intron 45.0%
TGCCTTAATTAGGCAGCTAG+GGG + chr3_4:77335926-77335945 None:intergenic 45.0%
CTTCTCTATAGGCATAGCCA+TGG - chr3_4:77336078-77336097 Msa0523890:intron 45.0%
GCCGTCAAAGATGAAAGTGA+TGG - chr3_4:77336263-77336282 Msa0523890:CDS 45.0%
GTAACCATCACTGTTTCCCT+AGG - chr3_4:77336386-77336405 Msa0523890:CDS 45.0%
ACTACCTAGGGAAACAGTGA+TGG + chr3_4:77336393-77336412 None:intergenic 45.0%
ATCACTGTTTCCCTAGGTAG+TGG - chr3_4:77336392-77336411 Msa0523890:CDS 45.0%
AACACATCCTCCACTACCTA+GGG + chr3_4:77336405-77336424 None:intergenic 45.0%
AAACACATCCTCCACTACCT+AGG + chr3_4:77336406-77336425 None:intergenic 45.0%
AAGGACCCGATGAGGATATA+TGG + chr3_4:77336466-77336485 None:intergenic 45.0%
!! TATAAATTGTAAAAAATAAA+AGG - chr3_4:77334365-77334384 Msa0523890:intron 5.0%
CAAGAGATGTTCGTGTCCGT+TGG - chr3_4:77333566-77333585 Msa0523890:three_prime_UTR 50.0%
TTCGTGTCCGTTGGTACTAC+TGG - chr3_4:77333575-77333594 Msa0523890:three_prime_UTR 50.0%
GCGTCATTTCCATGGCTCAA+AGG - chr3_4:77333621-77333640 Msa0523890:three_prime_UTR 50.0%
AATCAGTGCAGCGATCACAC+TGG + chr3_4:77334026-77334045 None:intergenic 50.0%
! AGTGTGATCGCTGCACTGAT+TGG - chr3_4:77334025-77334044 Msa0523890:intron 50.0%
CGGGTGCGAAAGACATATCA+TGG + chr3_4:77334477-77334496 None:intergenic 50.0%
ATATGTCTTTCGCACCCGTC+TGG - chr3_4:77334479-77334498 Msa0523890:intron 50.0%
!! CTTCCTTAGCTCTAAGGTCC+TGG - chr3_4:77334601-77334620 Msa0523890:intron 50.0%
GGATAAGTGCTCTTCGGATG+TGG + chr3_4:77335091-77335110 None:intergenic 50.0%
TGGGCAGCTGTTTGATCCTT+AGG + chr3_4:77335112-77335131 None:intergenic 50.0%
TATCAAAGATCTCGGAGGCC+AGG + chr3_4:77335508-77335527 None:intergenic 50.0%
!! CAACACAACGAAGGTGTTGG+AGG + chr3_4:77335648-77335667 None:intergenic 50.0%
CTCCAACACCTTCGTTGTGT+TGG - chr3_4:77335646-77335665 Msa0523890:intron 50.0%
! ACCCAACACAACGAAGGTGT+TGG + chr3_4:77335651-77335670 None:intergenic 50.0%
CTTCACACCCAACACAACGA+AGG + chr3_4:77335657-77335676 None:intergenic 50.0%
TCGTTGTGTTGGGTGTGAAG+AGG - chr3_4:77335657-77335676 Msa0523890:intron 50.0%
TCCCACTTTGCCTGGACATA+TGG - chr3_4:77335689-77335708 Msa0523890:intron 50.0%
GCCATATGTCCAGGCAAAGT+GGG + chr3_4:77335693-77335712 None:intergenic 50.0%
TGCCATATGTCCAGGCAAAG+TGG + chr3_4:77335694-77335713 None:intergenic 50.0%
CTTAATTAGGCAGCTAGGGG+AGG + chr3_4:77335923-77335942 None:intergenic 50.0%
CTCCCCTAGCTGCCTAATTA+AGG - chr3_4:77335921-77335940 Msa0523890:intron 50.0%
GGGATGCATAGCTGCAATGA+AGG + chr3_4:77335958-77335977 None:intergenic 50.0%
! ACTGTTTCCCTAGGTAGTGG+AGG - chr3_4:77336395-77336414 Msa0523890:CDS 50.0%
GTATGAACAAGGACCCGATG+AGG + chr3_4:77336474-77336493 None:intergenic 50.0%
CAACTGCCAACACGATTCAC+TGG + chr3_4:77336567-77336586 None:intergenic 50.0%
! CAGTGAATCGTGTTGGCAGT+TGG - chr3_4:77336565-77336584 Msa0523890:CDS 50.0%
GCATGCGTTCCTCTAAACCG+TGG - chr3_4:77333500-77333519 Msa0523890:three_prime_UTR 55.0%
TGTCCGTTGGTACTACTGGC+CGG - chr3_4:77333579-77333598 Msa0523890:three_prime_UTR 55.0%
TGGCCGGAGGAGACAAAGAA+GGG - chr3_4:77333595-77333614 Msa0523890:three_prime_UTR 55.0%
CGACCCTTCTTTGTCTCCTC+CGG + chr3_4:77333601-77333620 None:intergenic 55.0%
CGGAGGAGACAAAGAAGGGT+CGG - chr3_4:77333599-77333618 Msa0523890:three_prime_UTR 55.0%
AAGGGTCGGCGTCATTTCCA+TGG - chr3_4:77333613-77333632 Msa0523890:three_prime_UTR 55.0%
!! AGGAGTGAGAGCACCAGTAG+CGG + chr3_4:77333785-77333804 None:intergenic 55.0%
CTCCCAGGACCTTAGAGCTA+AGG + chr3_4:77334607-77334626 None:intergenic 55.0%
!! CTTAGCTCTAAGGTCCTGGG+AGG - chr3_4:77334605-77334624 Msa0523890:intron 55.0%
GGGTGAGTAGTGACGATGAG+TGG + chr3_4:77335013-77335032 None:intergenic 55.0%
CTTCGGATGTGGCTCAGGTT+TGG + chr3_4:77335080-77335099 None:intergenic 55.0%
ATGCTGCAGTTAGGCGGCTA+GGG + chr3_4:77335605-77335624 None:intergenic 55.0%
ACAACCCGGATGCTGCAGTT+AGG + chr3_4:77335614-77335633 None:intergenic 55.0%
CCTCCGGCCAGTAGTACCAA+CGG + chr3_4:77333585-77333604 None:intergenic 60.0%
CTGGCCGGAGGAGACAAAGA+AGG - chr3_4:77333594-77333613 Msa0523890:three_prime_UTR 60.0%
GTGCTCTTCGGATGTGGCTC+AGG + chr3_4:77335085-77335104 None:intergenic 60.0%
TGCTGCAGTTAGGCGGCTAG+GGG + chr3_4:77335604-77335623 None:intergenic 60.0%
GATGCTGCAGTTAGGCGGCT+AGG + chr3_4:77335606-77335625 None:intergenic 60.0%
AGCCGCCTAACTGCAGCATC+CGG - chr3_4:77335606-77335625 Msa0523890:intron 60.0%
ACCCGGATGCTGCAGTTAGG+CGG + chr3_4:77335611-77335630 None:intergenic 60.0%
CCGTTGGTACTACTGGCCGG+AGG - chr3_4:77333582-77333601 Msa0523890:three_prime_UTR 65.0%
GCCGCCTAACTGCAGCATCC+GGG - chr3_4:77335607-77335626 Msa0523890:intron 65.0%
GCTAGGGGAGGTCGCAAGGA+TGG + chr3_4:77335911-77335930 None:intergenic 65.0%
GGCAGCTAGGGGAGGTCGCA+AGG + chr3_4:77335915-77335934 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 77333472 77336588 77333472 ID=Msa0523890;Name=Msa0523890
chr3_4 mRNA 77333472 77336588 77333472 ID=Msa0523890-mRNA-1;Parent=Msa0523890;Name=Msa0523890-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0.33|0.25|1|1|1|4|312|192
chr3_4 exon 77333472 77333810 77333472 ID=Msa0523890-mRNA-1:exon:22654;Parent=Msa0523890-mRNA-1
chr3_4 exon 77334632 77334767 77334632 ID=Msa0523890-mRNA-1:exon:22653;Parent=Msa0523890-mRNA-1
chr3_4 exon 77336014 77336082 77336014 ID=Msa0523890-mRNA-1:exon:22652;Parent=Msa0523890-mRNA-1
chr3_4 exon 77336242 77336588 77336242 ID=Msa0523890-mRNA-1:exon:22651;Parent=Msa0523890-mRNA-1
chr3_4 CDS 77336242 77336588 77336242 ID=Msa0523890-mRNA-1:cds;Parent=Msa0523890-mRNA-1
chr3_4 CDS 77336014 77336082 77336014 ID=Msa0523890-mRNA-1:cds;Parent=Msa0523890-mRNA-1
chr3_4 CDS 77334632 77334767 77334632 ID=Msa0523890-mRNA-1:cds;Parent=Msa0523890-mRNA-1
chr3_4 CDS 77333784 77333810 77333784 ID=Msa0523890-mRNA-1:cds;Parent=Msa0523890-mRNA-1
chr3_4 three_prime_UTR 77333472 77333783 77333472 ID=Msa0523890-mRNA-1:three_prime_utr;Parent=Msa0523890-mRNA-1
Gene Sequence

>Msa0523890

TTGTTTGTTTCAGAGGGTGATTGTGTACGCATGCGTTCCTCTAAACCGTGGATACCAACATATGTGGCTATGATCGAAGAGATGAAAGCTGATTCAAGAGATGTTCGTGTCCGTTGGTACTACTGGCCGGAGGAGACAAAGAAGGGTCGGCGTCATTTCCATGGCTCAAAGGAGCTTTTTCTATCTGATCATTTAGATGTTCAGAGTGTTGATACAATCGAAGGAAAATGCACTGTTCACAGTTTGGAGAACTACATGAAGCTTGATGTTGTTGGTGATGATGATTTCTTCTGTCGATTGAACTACAATTCCGCTACTGGTGCTCTCACTCCTGATATAGTTCAAGTCTATTGTAAATGTGAGATGCCCTACAACCCTGATGAAGTTATGATCCAGTGTGATCGCTGCACTGATTGGTTTCATCCTGCTTGTATAGACATGACGGTGGAAGAAGCCGAAAGAATTGACAACTTCATTTGTGAAAGTTGTTCTGCCGAAGTTCAAAAAAAGTTGCAAGATTCTCATTCTGCTGCAAGACTCCCAAATACAAAGGTGGATACTAAACGCCGTCAAAGATGA
Protein sequence

>Msa0523890

LFVSEGDCVRMRSSKPWIPTYVAMIEEMKADSRDVRVRWYYWPEETKKGRRHFHGSKELFLSDHLDVQSVDTIEGKCTVHSLENYMKLDVVGDDDFFCRLNYNSATGALTPDIVQVYCKCEMPYNPDEVMIQCDRCTDWFHPACIDMTVEEAERIDNFICESCSAEVQKKLQDSHSAARLPNTKVDTKRRQR*