AlfalfaGEDB | Msa0527650

M. sativa cultivar zhongmu-4 / Msa0527650

KEGG: K09184
GO: GO:0000976 GO:0000977 GO:0000981 GO:0001012 GO:0001067 GO:0001085 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00072.27

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527650	AES76910.1	84.810	158	19	1	17	169	54	211	1.03e-88	268

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527650	sp\|Q8H7S7\|ORR21_ORYSJ	35.417	96	59	1	1	93	43	138	2.62e-14	73.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527650	G7KPX8	84.810	158	19	1	17	169	54	211	4.93e-89	268

TFs/TRs:

Gene ID	Type	Classification
Msa0527650	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527650	MtrunA17_Chr6g0473731	78.161	87	17	1	47	133	10	94	1.40e-40	141
Msa0527650	MtrunA17_Chr7g0223971	70.968	62	18	0	49	110	1	62	2.24e-24	98.6
Msa0527650	MtrunA17_Chr4g0067981	36.458	96	58	1	1	93	62	157	2.14e-15	72.8
Msa0527650	MtrunA17_Chr2g0294911	34.737	95	59	1	1	92	60	154	1.50e-14	70.5
Msa0527650	MtrunA17_Chr3g0134481	30.000	80	56	0	14	93	55	134	1.25e-11	60.1
Msa0527650	MtrunA17_Chr1g0161431	32.468	77	52	0	17	93	61	137	5.90e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0527650	AT2G01760.1	34.783	92	56	2	3	90	40	131	9.49e-15	71.2
Msa0527650	AT2G01760.2	34.783	92	56	2	3	90	68	159	1.03e-14	71.2
Msa0527650	AT3G16857.1	29.167	120	80	2	1	117	64	181	3.83e-13	66.6
Msa0527650	AT3G16857.2	29.167	120	80	2	1	117	64	181	3.85e-13	66.6
Msa0527650	AT3G16857.3	30.693	101	68	1	17	117	13	111	1.13e-12	65.5
Msa0527650	AT2G01760.3	40.625	64	37	1	28	90	3	66	2.70e-11	60.8

Find 26 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGGTGAGTTTCATATATT+TGG	0.084119	4_1:+89596	Msa0527650:CDS
TATATGAAACTCACCAATTA+TGG	0.162095	4_1:-89591	None:intergenic
CTGAAGACTTTAGAGATATT+TGG	0.199417	4_1:+89932	Msa0527650:CDS
AAAGATGCTCATCATGCTTT+TGG	0.277026	4_1:+89546	Msa0527650:CDS
CTAAAGTCTTCAGGACAAAA+TGG	0.299256	4_1:-89922	None:intergenic
CACCAAGATGAAGGGGAGTT+TGG	0.304158	4_1:+90072	Msa0527650:CDS
AAAAGCATGATGAGCATCTT+TGG	0.320559	4_1:-89544	None:intergenic
ATTCATCTTGAATTTGCATT+TGG	0.327017	4_1:-89637	None:intergenic
CAAATATCTCTAAAGTCTTC+AGG	0.332147	4_1:-89931	None:intergenic
TTTCATATATTTGGAATGAA+TGG	0.333235	4_1:+89605	Msa0527650:CDS
GAATTTCAACTTCCTGTCAT+AGG	0.416860	4_1:+89656	Msa0527650:CDS
TTTCAAACTCCCCTTCATCT+TGG	0.434656	4_1:+90146	Msa0527650:CDS
ATTTATTGATAATTTATTTG+CGG	0.447669	4_1:+89987	Msa0527650:CDS
CTCCAAACTCCCCTTCATCT+TGG	0.455112	4_1:-90074	None:intergenic
ATATGAAACTCACCAATTAT+GGG	0.459383	4_1:-89590	None:intergenic
GTTTGGAGTTCTTAAGATGA+AGG	0.465754	4_1:+90089	Msa0527650:CDS
TTTGGAGTTCTTAAGATGAA+GGG	0.466874	4_1:+90090	Msa0527650:CDS
TTGGAGTTCTTAAGATGAAG+GGG	0.503912	4_1:+90091	Msa0527650:CDS
TGCTCCATGTTCTAAAGTCT+TGG	0.513882	4_1:-89881	None:intergenic
TGTGATGATTGCCAAGATGA+AGG	0.549385	4_1:-90157	None:intergenic
AAATAATACTTACCTATGAC+AGG	0.557435	4_1:-89668	None:intergenic
AGATATTTGGAAGTATGCAG+TGG	0.573012	4_1:+89945	Msa0527650:CDS
GTGATGATTGCCAAGATGAA+GGG	0.592645	4_1:-90156	None:intergenic
ATCTCCAAGACTTTAGAACA+TGG	0.647039	4_1:+89877	Msa0527650:CDS
TGATGATTGCCAAGATGAAG+GGG	0.650218	4_1:-90155	None:intergenic
GATGTCTAAAGACAATAGAG+AGG	0.681369	4_1:+89849	Msa0527650:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATTTTTTTTTTTTTGGAA+GGG	-	chr4_1:89696-89715	None:intergenic	10.0%
!!!	TTAATTTTTTTTTTTTTGGA+AGG	-	chr4_1:89697-89716	None:intergenic	10.0%
!!!	AGACTTAATTTTTTTTTTTT+TGG	-	chr4_1:89701-89720	None:intergenic	10.0%
!!!	ATTTATTGATAATTTATTTG+CGG	+	chr4_1:89987-90006	Msa0527650:CDS	10.0%
!!!	TTTCATATATTTGGAATGAA+TGG	+	chr4_1:89605-89624	Msa0527650:CDS	20.0%
!!	AAAATAAAAAAGCAATTGCA+AGG	-	chr4_1:89745-89764	None:intergenic	20.0%
!	ATATGAAACTCACCAATTAT+GGG	-	chr4_1:89593-89612	None:intergenic	25.0%
!	TATATGAAACTCACCAATTA+TGG	-	chr4_1:89594-89613	None:intergenic	25.0%
!	ATTGGTGAGTTTCATATATT+TGG	+	chr4_1:89596-89615	Msa0527650:CDS	25.0%
!	ATTCATCTTGAATTTGCATT+TGG	-	chr4_1:89640-89659	None:intergenic	25.0%
!	AAATAATACTTACCTATGAC+AGG	-	chr4_1:89671-89690	None:intergenic	25.0%
!	TGAAACAAAAAGGTAAACAA+GGG	-	chr4_1:89823-89842	None:intergenic	25.0%
!	TTGAAACAAAAAGGTAAACA+AGG	-	chr4_1:89824-89843	None:intergenic	25.0%
!!	CATCACTATTTGAAACAAAA+AGG	-	chr4_1:89833-89852	None:intergenic	25.0%
!	GAAACTTTTGATCCCATAAT+TGG	+	chr4_1:89578-89597	Msa0527650:CDS	30.0%
!!	CAAATATCTCTAAAGTCTTC+AGG	-	chr4_1:89934-89953	None:intergenic	30.0%
!	CTGAAGACTTTAGAGATATT+TGG	+	chr4_1:89932-89951	Msa0527650:CDS	30.0%
	GTAAAAATCACCAAGATGAA+GGG	+	chr4_1:90064-90083	Msa0527650:CDS	30.0%
	TAAAAATCACCAAGATGAAG+GGG	+	chr4_1:90065-90084	Msa0527650:CDS	30.0%
!	TTTGGAGTTCTTAAGATGAA+GGG	+	chr4_1:90090-90109	Msa0527650:CDS	30.0%
!	AAAAGCATGATGAGCATCTT+TGG	-	chr4_1:89547-89566	None:intergenic	35.0%
!	AAAGATGCTCATCATGCTTT+TGG	+	chr4_1:89546-89565	Msa0527650:CDS	35.0%
	GAATTTCAACTTCCTGTCAT+AGG	+	chr4_1:89656-89675	Msa0527650:CDS	35.0%
	GATGTCTAAAGACAATAGAG+AGG	+	chr4_1:89849-89868	Msa0527650:CDS	35.0%
!!	ATCTCCAAGACTTTAGAACA+TGG	+	chr4_1:89877-89896	Msa0527650:CDS	35.0%
	CTAAAGTCTTCAGGACAAAA+TGG	-	chr4_1:89925-89944	None:intergenic	35.0%
!	AGATATTTGGAAGTATGCAG+TGG	+	chr4_1:89945-89964	Msa0527650:CDS	35.0%
	CGTAAAAATCACCAAGATGA+AGG	+	chr4_1:90063-90082	Msa0527650:CDS	35.0%
!	GTTTGGAGTTCTTAAGATGA+AGG	+	chr4_1:90089-90108	Msa0527650:CDS	35.0%
	TTGGAGTTCTTAAGATGAAG+GGG	+	chr4_1:90091-90110	Msa0527650:CDS	35.0%
!!	TGCTCCATGTTCTAAAGTCT+TGG	-	chr4_1:89884-89903	None:intergenic	40.0%
	TTTCAAACTCCCCTTCATCT+TGG	+	chr4_1:90146-90165	Msa0527650:CDS	40.0%
	TGATGATTGCCAAGATGAAG+GGG	-	chr4_1:90158-90177	None:intergenic	40.0%
	GTGATGATTGCCAAGATGAA+GGG	-	chr4_1:90159-90178	None:intergenic	40.0%
	TGTGATGATTGCCAAGATGA+AGG	-	chr4_1:90160-90179	None:intergenic	40.0%
	CACCAAGATGAAGGGGAGTT+TGG	+	chr4_1:90072-90091	Msa0527650:CDS	50.0%
	CTCCAAACTCCCCTTCATCT+TGG	-	chr4_1:90077-90096	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	89536	90215	89536	ID=Msa0527650;Name=Msa0527650
chr4_1	mRNA	89536	90215	89536	ID=Msa0527650-mRNA-1;Parent=Msa0527650;Name=Msa0527650-mRNA-1;_AED=0.06;_eAED=0.11;_QI=0\|0\|0\|1\|1\|1\|2\|0\|169
chr4_1	exon	89536	89677	89536	ID=Msa0527650-mRNA-1:exon:5;Parent=Msa0527650-mRNA-1
chr4_1	exon	89848	90215	89848	ID=Msa0527650-mRNA-1:exon:6;Parent=Msa0527650-mRNA-1
chr4_1	CDS	89536	89677	89536	ID=Msa0527650-mRNA-1:cds;Parent=Msa0527650-mRNA-1
chr4_1	CDS	89848	90215	89848	ID=Msa0527650-mRNA-1:cds;Parent=Msa0527650-mRNA-1

Gene Sequence

>Msa0527650

TCAAACTGCCAAAGATGCTCATCATGCTTTTGGTTACTCTTCGAAACTTTTGATCCCATAATTGGTGAGTTTCATATATTTGGAATGAATGGATTTGAATTCCAAATGCAAATTCAAGATGAATTTCAACTTCCTGTCATAGTGATGTCTAAAGACAATAGAGAGGACATAATCTCCAAGACTTTAGAACATGGAGCAGCACACTACATTGTTAAACCATTTTGTCCTGAAGACTTTAGAGATATTTGGAAGTATGCAGTGGAAGCGAAGAAAAATAAACTATTTATTGATAATTTATTTGCGGCAAGTGAAGAAGAAGAAACATCAACTAATCAACTACAAACAAAAAAGAAATGTCGTAAAAATCACCAAGATGAAGGGGAGTTTGGAGTTCTTAAGATGAAGGGGAGTTGTGATGATTTTACTACATTTCTTAGTAATTTCAAACTCCCCTTCATCTTGGCAATCATCACAACTCTTTCTTTTACTACATTTCTTATTTGTTTGTAG

Protein sequence

>Msa0527650

SNCQRCSSCFWLLFETFDPIIGEFHIFGMNGFEFQMQIQDEFQLPVIVMSKDNREDIISKTLEHGAAHYIVKPFCPEDFRDIWKYAVEAKKNKLFIDNLFAASEEEETSTNQLQTKKKCRKNHQDEGEFGVLKMKGSCDDFTTFLSNFKLPFILAIITTLSFTTFLICL*