AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0527760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0527760 MtrunA17_Chr5g0396901 97.312 893 24 0 1 893 25 917 0.0 1804
Msa0527760 MtrunA17_Chr8g0347481 53.333 600 246 8 1 595 1 571 0.0 635
Msa0527760 MtrunA17_Chr4g0042731 29.773 309 168 15 247 539 142 417 8.22e-25 108
Msa0527760 MtrunA17_Chr4g0075541 31.474 251 130 9 280 525 131 344 1.27e-24 107
Msa0527760 MtrunA17_Chr4g0059121 29.845 258 142 10 282 536 104 325 1.18e-23 103
Msa0527760 MtrunA17_Chr4g0065211 30.556 288 150 11 264 535 1139 1392 2.16e-23 107
Msa0527760 MtrunA17_Chr3g0117731 30.233 258 141 9 282 536 103 324 2.22e-23 102
Msa0527760 MtrunA17_Chr5g0406251 26.104 249 146 8 282 528 337 549 5.12e-23 104
Msa0527760 MtrunA17_Chr4g0047191 27.273 253 145 10 282 531 92 308 3.10e-22 99.4
Msa0527760 MtrunA17_Chr4g0047211 27.273 253 145 10 282 531 87 303 3.85e-22 99.0
Msa0527760 MtrunA17_Chr4g0047201 26.616 263 155 9 282 541 248 475 5.44e-22 100
Msa0527760 MtrunA17_Chr2g0305141 29.373 303 166 12 264 549 1101 1372 7.50e-22 102
Msa0527760 MtrunA17_Chr5g0397931 28.013 307 165 16 246 534 421 689 8.25e-22 101
Msa0527760 MtrunA17_Chr1g0211611 26.908 249 144 8 282 528 343 555 8.88e-22 100
Msa0527760 MtrunA17_Chr2g0282321 25.954 262 158 9 282 541 209 436 7.87e-21 96.7
Msa0527760 MtrunA17_Chr6g0461841 31.618 272 152 8 270 536 1001 1243 8.25e-21 99.0
Msa0527760 MtrunA17_Chr6g0450871 28.137 263 143 10 279 526 118 349 1.14e-20 95.1
Msa0527760 MtrunA17_Chr7g0244621 29.134 254 143 9 278 523 82 306 1.60e-20 94.4
Msa0527760 MtrunA17_Chr4g0059181 26.996 263 146 8 279 526 135 366 1.67e-20 94.7
Msa0527760 MtrunA17_Chr3g0143531 27.092 251 145 9 282 530 67 281 1.94e-20 92.8
Msa0527760 MtrunA17_Chr2g0310761 27.687 307 166 12 242 526 933 1205 2.27e-20 97.4
Msa0527760 MtrunA17_Chr3g0117811 25.779 353 192 13 212 526 17 337 2.43e-20 94.0
Msa0527760 MtrunA17_Chr8g0357611 26.687 326 185 11 222 526 1344 1636 3.12e-20 97.1
Msa0527760 MtrunA17_Chr1g0202101 24.315 292 167 10 245 519 273 527 4.46e-20 95.1
Msa0527760 MtrunA17_Chr4g0072741 29.153 295 163 10 247 524 834 1099 6.48e-20 95.9
Msa0527760 MtrunA17_Chr2g0310781 27.213 305 166 13 242 524 839 1109 4.97e-18 89.7
Msa0527760 MtrunA17_Chr4g0068411 28.772 285 152 13 246 516 458 705 5.68e-18 89.0
Msa0527760 MtrunA17_Chr6g0449241 26.316 304 172 12 242 526 20 290 1.25e-17 85.5
Msa0527760 MtrunA17_Chr2g0294351 27.857 280 145 14 265 528 485 723 8.52e-17 85.5
Msa0527760 MtrunA17_Chr3g0090261 25.101 247 146 9 280 523 126 336 1.35e-16 82.8
Msa0527760 MtrunA17_Chr3g0116981 25.413 303 169 11 242 523 20 286 1.42e-16 82.4
Msa0527760 MtrunA17_Chr1g0196631 28.629 248 139 7 289 530 955 1170 1.81e-16 84.7
Msa0527760 MtrunA17_Chr5g0403721 26.531 245 150 9 279 523 28 242 4.05e-16 81.3
Msa0527760 MtrunA17_Chr1g0171571 28.696 230 115 9 326 526 106 315 5.34e-16 82.4
Msa0527760 MtrunA17_Chr5g0443471 27.881 269 156 11 280 541 205 442 9.29e-16 81.3
Msa0527760 MtrunA17_Chr5g0394681 27.461 193 111 6 334 526 201 364 4.52e-15 78.2
Msa0527760 MtrunA17_Chr1g0191171 24.252 301 173 13 246 530 687 948 5.45e-15 79.7
Msa0527760 MtrunA17_Chr5g0427651 25.581 301 165 15 247 528 645 905 1.09e-14 78.6
Msa0527760 MtrunA17_Chr1g0157231 25.789 190 105 6 282 470 188 342 1.93e-14 76.3
Msa0527760 MtrunA17_Chr8g0380551 28.226 248 136 11 282 519 266 481 3.55e-14 76.3
Msa0527760 MtrunA17_Chr1g0179331 24.497 298 172 12 247 528 554 814 8.09e-14 75.9
Msa0527760 MtrunA17_Chr3g0105891 25.820 244 142 8 285 523 371 580 1.29e-13 74.7
Msa0527760 MtrunA17_Chr5g0437741 29.694 229 114 9 282 509 536 718 1.80e-13 74.7
Msa0527760 MtrunA17_Chr4g0000431 23.288 292 182 10 243 521 19 281 2.29e-13 74.3
Msa0527760 MtrunA17_Chr1g0209511 26.766 269 150 11 277 524 352 594 5.88e-13 72.8
Msa0527760 MtrunA17_Chr3g0115171 26.778 239 137 8 277 513 322 524 1.51e-12 71.2
Msa0527760 MtrunA17_Chr1g0171451 28.000 275 149 13 265 526 433 671 1.80e-12 71.2
Msa0527760 MtrunA17_Chr4g0051191 27.273 242 134 8 277 514 203 406 3.86e-12 69.3
Msa0527760 MtrunA17_Chr6g0450571 23.675 283 178 9 243 514 11 266 6.30e-12 69.3
Msa0527760 MtrunA17_Chr8g0359691 26.733 303 147 16 255 523 674 935 7.03e-12 69.7
Msa0527760 MtrunA17_Chr4g0053411 24.702 336 170 14 239 523 458 761 8.04e-12 69.3
Msa0527760 MtrunA17_Chr2g0280191 26.190 252 131 11 241 469 676 895 1.24e-11 68.9
Msa0527760 MtrunA17_Chr1g0209521 24.752 303 166 13 255 527 368 638 1.83e-11 68.2
Msa0527760 MtrunA17_Chr8g0384301 26.293 232 133 8 285 514 349 544 3.27e-11 67.4
Msa0527760 MtrunA17_Chr4g0041201 26.316 285 160 13 257 531 559 803 4.53e-11 67.0
Msa0527760 MtrunA17_Chr5g0423121 25.769 260 144 11 277 521 377 602 5.61e-11 66.2
Msa0527760 MtrunA17_Chr7g0276571 26.592 267 138 12 280 523 457 688 6.50e-11 66.2
Msa0527760 MtrunA17_Chr3g0111551 26.728 217 122 8 285 499 113 294 7.69e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0527760 AT1G64300.2 52.510 777 297 14 1 767 1 715 0.0 785
Msa0527760 AT1G64300.1 52.510 777 297 14 1 767 1 715 0.0 785
Msa0527760 AT5G41730.2 52.291 742 295 11 1 726 1 699 0.0 764
Msa0527760 AT5G41730.1 52.291 742 295 11 1 726 1 699 0.0 764
Msa0527760 AT1G01450.1 53.590 390 151 7 211 594 2 367 1.45e-131 403
Msa0527760 AT1G14000.1 31.392 309 165 17 247 540 161 437 1.82e-24 107
Msa0527760 AT1G62400.1 30.645 248 131 8 280 523 130 340 5.47e-24 105
Msa0527760 AT3G46930.2 25.959 339 198 10 224 545 179 481 7.60e-24 107
Msa0527760 AT3G46930.1 25.959 339 198 10 224 545 144 446 1.02e-23 106
Msa0527760 AT3G46920.2 28.378 370 191 16 192 530 816 1142 1.09e-22 105
Msa0527760 AT3G46920.1 28.378 370 191 16 192 530 750 1076 1.18e-22 105
Msa0527760 AT5G58950.1 27.451 255 140 11 276 523 247 463 1.56e-21 100
Msa0527760 AT2G35050.1 28.618 304 166 14 242 526 968 1239 4.23e-21 100
Msa0527760 AT2G35050.2 28.618 304 166 14 242 526 968 1239 4.23e-21 100
Msa0527760 AT5G01850.1 27.642 246 145 7 282 523 63 279 1.15e-20 94.7
Msa0527760 AT4G38470.2 25.794 252 149 8 282 531 332 547 2.81e-20 96.3
Msa0527760 AT5G57610.1 27.723 303 173 10 247 532 780 1053 3.88e-20 97.1
Msa0527760 AT5G01850.2 29.907 214 120 6 314 523 116 303 1.10e-19 92.4
Msa0527760 AT4G35780.1 24.590 244 146 8 282 523 334 541 1.33e-19 94.4
Msa0527760 AT4G38470.1 25.506 247 146 8 282 526 332 542 1.63e-19 94.0
Msa0527760 AT5G50000.1 26.616 263 147 8 279 526 128 359 1.84e-19 92.0
Msa0527760 AT1G04700.1 30.380 237 137 6 289 524 821 1030 2.27e-19 94.4
Msa0527760 AT1G04700.2 30.380 237 137 6 289 524 821 1030 2.27e-19 94.4
Msa0527760 AT3G01490.1 28.244 262 144 11 279 526 154 385 2.28e-19 92.0
Msa0527760 AT2G17700.1 26.000 250 145 10 282 528 328 540 2.86e-19 93.2
Msa0527760 AT1G79570.2 26.403 303 175 10 242 526 958 1230 3.57e-19 94.0
Msa0527760 AT1G79570.1 26.403 303 175 10 242 526 958 1230 3.57e-19 94.0
Msa0527760 AT1G16270.1 27.143 280 167 8 258 533 890 1136 4.03e-19 93.6
Msa0527760 AT1G16270.2 27.143 280 167 8 258 533 890 1136 4.03e-19 93.6
Msa0527760 AT1G16270.3 27.143 280 167 8 258 533 890 1136 4.03e-19 93.6
Msa0527760 AT3G24715.1 28.873 284 172 9 243 522 842 1099 4.40e-19 93.6
Msa0527760 AT3G24715.3 28.873 284 172 9 243 522 842 1099 4.40e-19 93.6
Msa0527760 AT3G24715.2 28.873 284 172 9 243 522 842 1099 4.40e-19 93.6
Msa0527760 AT4G31170.7 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa0527760 AT4G31170.6 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa0527760 AT4G31170.5 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa0527760 AT4G31170.1 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa0527760 AT4G31170.2 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa0527760 AT4G31170.3 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa0527760 AT4G31170.8 27.092 251 136 9 280 523 175 385 5.92e-19 90.9
Msa0527760 AT3G50730.1 25.403 248 156 8 277 523 77 296 2.20e-18 88.6
Msa0527760 AT3G06630.2 28.283 297 160 15 247 528 433 691 2.71e-18 90.5
Msa0527760 AT4G23050.2 27.815 302 158 14 246 528 466 726 2.73e-18 90.5
Msa0527760 AT4G23050.1 27.815 302 158 14 246 528 465 725 2.77e-18 90.5
Msa0527760 AT5G66710.1 25.641 273 163 8 278 544 113 351 4.37e-18 88.2
Msa0527760 AT2G40860.4 30.964 197 98 6 340 514 24 204 9.53e-18 88.2
Msa0527760 AT5G49470.3 27.857 280 145 14 265 528 510 748 1.06e-17 88.6
Msa0527760 AT5G49470.4 27.857 280 145 14 265 528 484 722 1.22e-17 88.6
Msa0527760 AT2G40860.2 30.144 209 108 6 340 526 122 314 1.39e-17 88.2
Msa0527760 AT3G27560.1 27.600 250 146 10 282 526 71 290 1.47e-17 85.9
Msa0527760 AT5G11850.1 25.503 298 171 12 246 529 607 867 1.53e-17 88.2
Msa0527760 AT2G40860.1 30.964 197 98 6 340 514 122 302 1.55e-17 87.8
Msa0527760 AT5G49470.7 27.857 280 145 14 265 528 383 621 1.71e-17 87.8
Msa0527760 AT2G40860.3 30.964 197 98 6 340 514 122 302 1.72e-17 87.8
Msa0527760 AT5G49470.1 27.857 280 145 14 265 528 223 461 2.25e-17 86.7
Msa0527760 AT5G40540.1 27.668 253 148 11 276 523 64 286 2.35e-17 85.1
Msa0527760 AT2G24360.1 26.050 238 143 7 286 523 180 384 2.43e-17 85.9
Msa0527760 AT3G22750.1 24.528 265 151 8 279 526 120 352 3.75e-17 85.1
Msa0527760 AT3G57830.3 27.737 274 162 10 279 527 398 660 5.85e-17 86.3
Msa0527760 AT3G57830.1 27.737 274 162 10 279 527 398 660 5.85e-17 86.3
Msa0527760 AT4G18950.2 28.571 252 138 10 282 526 200 416 9.52e-17 84.3
Msa0527760 AT4G18950.1 28.571 252 138 10 282 526 200 416 1.07e-16 84.3
Msa0527760 AT3G63260.1 24.060 266 155 8 279 526 129 365 1.10e-16 83.6
Msa0527760 AT4G24480.1 26.073 303 166 13 247 528 668 933 1.54e-16 85.1
Msa0527760 AT2G42290.1 28.731 268 159 9 282 527 389 646 1.92e-16 84.3
Msa0527760 AT5G50180.1 27.236 246 145 9 282 523 65 280 2.04e-16 82.4
Msa0527760 AT1G67890.1 27.273 275 152 13 265 528 506 743 2.09e-16 84.7
Msa0527760 AT5G40540.3 29.439 214 120 9 314 523 3 189 3.68e-16 79.7
Msa0527760 AT4G24480.2 25.589 297 170 13 247 528 668 928 4.71e-16 83.6
Msa0527760 AT4G24480.3 25.589 297 170 13 247 528 668 928 5.91e-16 83.2
Msa0527760 AT5G40540.2 29.439 214 120 9 314 523 24 210 7.35e-16 79.3
Msa0527760 AT3G06620.1 27.798 277 149 13 265 528 513 751 7.84e-16 82.8
Msa0527760 AT3G50720.1 26.848 257 159 9 276 531 88 316 1.63e-15 80.1
Msa0527760 AT3G58760.1 29.000 300 156 18 247 526 165 427 6.68e-15 79.0
Msa0527760 AT3G58760.3 29.000 300 156 18 247 526 228 490 8.60e-15 79.0
Msa0527760 AT3G58760.5 29.000 300 156 18 247 526 228 490 8.60e-15 79.0
Msa0527760 AT3G06630.1 28.261 276 145 15 247 507 433 670 8.90e-15 79.0
Msa0527760 AT4G14780.1 26.316 190 109 5 339 526 178 338 1.04e-14 77.4
Msa0527760 AT3G46930.4 24.715 263 149 8 224 473 179 405 3.26e-14 76.3
Msa0527760 AT3G46930.3 24.715 263 149 8 224 473 179 405 3.44e-14 76.3
Msa0527760 AT2G31800.1 25.987 304 177 14 243 530 188 459 3.99e-14 76.3
Msa0527760 AT3G46930.5 24.715 263 149 8 224 473 144 370 5.60e-14 75.5
Msa0527760 AT3G46930.6 24.715 263 149 8 224 473 83 309 7.38e-14 74.3
Msa0527760 AT3G58640.1 28.205 273 146 13 257 519 565 797 8.96e-14 76.3
Msa0527760 AT3G58640.2 28.205 273 146 13 257 519 565 797 8.96e-14 76.3
Msa0527760 AT1G50240.3 24.211 285 161 12 243 513 1 244 2.08e-13 75.1
Msa0527760 AT1G50240.2 24.211 285 161 12 243 513 1 244 2.08e-13 75.1
Msa0527760 AT5G66850.1 25.703 249 153 10 283 530 394 611 3.25e-13 74.3
Msa0527760 AT5G01890.1 23.743 358 209 13 191 527 646 960 3.44e-13 74.3
Msa0527760 AT5G66850.2 25.703 249 153 10 283 530 394 611 4.23e-13 73.9
Msa0527760 AT3G06630.4 28.571 238 120 13 247 470 433 634 4.89e-13 73.6
Msa0527760 AT3G06630.3 28.571 238 120 13 247 470 433 634 4.89e-13 73.6
Msa0527760 AT3G06630.5 28.571 238 120 13 247 470 433 634 4.89e-13 73.6
Msa0527760 AT3G59830.1 27.586 290 162 13 247 519 193 451 5.14e-13 72.8
Msa0527760 AT3G06630.6 28.571 238 120 13 247 470 349 550 5.60e-13 73.2
Msa0527760 AT5G18700.1 25.847 236 142 10 285 520 46 248 7.89e-13 73.2
Msa0527760 AT1G67890.3 27.907 258 130 15 265 507 506 722 3.25e-12 70.9
Msa0527760 AT1G67890.2 27.907 258 130 15 265 507 506 722 3.25e-12 70.9
Msa0527760 AT1G67890.4 27.907 258 130 15 265 507 378 594 4.24e-12 70.5
Msa0527760 AT2G31010.1 25.685 292 159 13 257 538 531 774 5.09e-12 70.5
Msa0527760 AT2G31010.2 25.685 292 159 13 257 538 531 774 5.09e-12 70.5
Msa0527760 AT3G53930.2 27.586 261 134 13 247 491 19 240 6.29e-12 70.1
Msa0527760 AT3G53930.1 27.586 261 134 13 247 491 19 240 6.67e-12 70.1
Msa0527760 AT5G49470.5 28.182 220 104 12 265 469 510 690 9.86e-12 69.3
Msa0527760 AT5G49470.6 28.182 220 104 12 265 469 510 690 1.08e-11 69.3
Msa0527760 AT2G42640.1 28.044 271 141 13 265 524 547 774 1.15e-11 69.3
Msa0527760 AT2G42640.4 28.044 271 141 13 265 524 509 736 1.39e-11 68.9
Msa0527760 AT5G38210.3 24.739 287 162 10 280 544 391 645 1.69e-11 68.6
Msa0527760 AT5G38210.2 25.175 286 162 10 280 544 409 663 1.75e-11 68.6
Msa0527760 AT5G07140.1 26.014 296 177 13 242 528 321 583 2.79e-11 67.8
Msa0527760 AT3G01490.2 27.632 228 122 10 279 492 154 352 3.19e-11 66.6
Msa0527760 AT4G08480.1 26.172 256 147 9 261 514 529 744 3.25e-11 67.8
Msa0527760 AT1G69270.1 25.820 244 144 10 270 510 291 500 4.83e-11 67.0
Msa0527760 AT5G40540.4 28.061 196 114 8 276 469 64 234 5.18e-11 65.1
Msa0527760 AT5G38210.1 25.175 286 162 10 280 544 397 651 5.51e-11 67.0
Msa0527760 AT4G08500.1 26.336 262 153 9 255 514 353 576 6.00e-11 66.6
Msa0527760 AT3G02880.1 31.500 200 99 9 272 467 375 540 6.09e-11 66.6
Msa0527760 AT3G19700.1 28.497 193 105 8 282 469 730 894 7.65e-11 66.6

Find 163 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCTGCTTTGATATTT+TGG 0.080066 4_1:+385106 None:intergenic
CTCTCAGATTCTGAGACTTT+TGG 0.146039 4_1:+384272 None:intergenic
ATCAAACTCACAAGATATTT+TGG 0.164131 4_1:+385060 None:intergenic
TTGATTCCCTTTGTTAATAT+TGG 0.196770 4_1:+384244 None:intergenic
CAAGCAAAAGTTGTTGGATT+TGG 0.204645 4_1:-385387 Msa0527760:CDS
GAGATTTGGCAAACAATATT+TGG 0.205668 4_1:-386060 Msa0527760:CDS
GATCCTAACAAAACACTAAT+TGG 0.207436 4_1:+385831 None:intergenic
AATTGCTTGCAAATTTCTTT+TGG 0.212048 4_1:+386035 None:intergenic
TCTCCTCACTTGGTAATCTT+TGG 0.230174 4_1:+385809 None:intergenic
GCTTATAAAGTTGTTGAAAA+AGG 0.240086 4_1:-384787 Msa0527760:CDS
GTGTCATCAGAAATTGATTT+TGG 0.241755 4_1:+384592 None:intergenic
GTAGGATATTACAAGGATTA+AGG 0.243595 4_1:+385442 None:intergenic
TATTCCTACTATAGATAATA+AGG 0.252638 4_1:-384731 Msa0527760:CDS
AAGAGATTAACCCTATAATT+TGG 0.257480 4_1:-385293 Msa0527760:CDS
GTCCTCTTAGAGAGCTTTCC+AGG 0.263161 4_1:-386343 Msa0527760:CDS
AACTGACCTTGGTCTTTCTT+TGG 0.265277 4_1:+384502 None:intergenic
GGTTACCAGATCTTTAATTA+TGG 0.266121 4_1:+384618 None:intergenic
CATAGATATTGCAATATGTT+TGG 0.282076 4_1:+385660 None:intergenic
TCAGGTGCATACCAAATTAT+AGG 0.292831 4_1:+385282 None:intergenic
CTGGTTTCTCATCTATTTGT+AGG 0.294229 4_1:-385002 Msa0527760:CDS
TTTGTCTTTAACCTGTTAAA+TGG 0.297516 4_1:+384119 None:intergenic
CGGCACATTGGTGAGGTTCT+TGG 0.303933 4_1:-386530 Msa0527760:CDS
TATGGTCATGTCTCAGATTA+AGG 0.304997 4_1:-383792 None:intergenic
TGGAAGTCTAAAAGCGCTTA+TGG 0.306735 4_1:-386510 Msa0527760:CDS
TGAAACATGGCAGCATCTTC+TGG 0.314429 4_1:+383962 None:intergenic
AAAAGAAATTTGCAAGCAAT+TGG 0.319977 4_1:-386033 Msa0527760:CDS
AATATACATTAAGCATTGTT+TGG 0.325498 4_1:-386303 Msa0527760:CDS
GGATTGTCATCTGTTGAAAA+TGG 0.333696 4_1:-385366 Msa0527760:CDS
ATTCTTTGAAGAAGTGAGTT+TGG 0.334226 4_1:+385947 None:intergenic
GTCCTTTGATTGCTCACATA+TGG 0.339488 4_1:+383876 None:intergenic
AGCATTGTGAGGATGCAAAA+AGG 0.341134 4_1:-384026 Msa0527760:CDS
CATTATCTGGTCGGGTTTCA+AGG 0.346406 4_1:-384358 Msa0527760:CDS
TTGACACGTCTTATTCTTGT+TGG 0.355333 4_1:+384062 None:intergenic
GAAATTGATGAATGGTAATT+CGG 0.363355 4_1:-385895 Msa0527760:CDS
TCGGGTTTCAAGGACAAATA+AGG 0.364030 4_1:-384348 Msa0527760:CDS
TTTAAGGAAATTCAATGGTT+AGG 0.370820 4_1:-385765 Msa0527760:CDS
GAGACCAATCACCATTTAAC+AGG 0.371640 4_1:-384130 Msa0527760:CDS
ATTGTTTGGATTCAAAAGAT+TGG 0.378122 4_1:-386289 Msa0527760:CDS
GTGAGAAAGTGATAACAAAT+TGG 0.378799 4_1:+385683 None:intergenic
TTCTTTGGCTTCATTTGTGA+TGG 0.379395 4_1:+384517 None:intergenic
TTAACCTGTTAAATGGTGAT+TGG 0.382051 4_1:+384126 None:intergenic
ATAGATATTGCAATATGTTT+GGG 0.387928 4_1:+385661 None:intergenic
AGTTCAAGCTGATTTAGTTC+AGG 0.389760 4_1:+384922 None:intergenic
ATCTCATGCTTCAAATGGCC+AGG 0.399868 4_1:-385506 Msa0527760:CDS
TTGAAGACAGAACTACAAAA+TGG 0.399911 4_1:-386379 Msa0527760:CDS
ATTGTAACATTCTTCTTTGA+CGG 0.401100 4_1:+384547 None:intergenic
TTGGATTCAAAAGATTGGTC+TGG 0.401687 4_1:-386284 Msa0527760:CDS
TGAGGAGAAGATTGGGAAGG+GGG 0.403016 4_1:-385794 Msa0527760:CDS
GGGTTAATCTCTTCACCAAT+TGG 0.403263 4_1:+385303 None:intergenic
CCATATGTTGTTCTTACATA+AGG 0.409101 4_1:+383810 None:intergenic
TACCAAGTGAGGAGAAGATT+GGG 0.409714 4_1:-385801 Msa0527760:CDS
ACAGTAGCCACATCCTTTGT+TGG 0.413590 4_1:+384694 None:intergenic
TCATTACCACCAACATTATC+TGG 0.424357 4_1:-384371 Msa0527760:CDS
ATGCTGCCATGTTTCAGATA+TGG 0.424537 4_1:-383955 Msa0527760:CDS
GAGAATGCTGGGGAGATGTC+AGG 0.427765 4_1:-386170 Msa0527760:CDS
TGGATTCAAAAGATTGGTCT+GGG 0.432597 4_1:-386283 Msa0527760:CDS
TTATAAGCAAACATTTCATA+AGG 0.434328 4_1:+384802 None:intergenic
CATCTTCACAAACTGTGCTT+TGG 0.436780 4_1:+384650 None:intergenic
TGCATCCATAATTAAAGATC+TGG 0.439192 4_1:-384623 Msa0527760:CDS
TTGTAACATTCTTCTTTGAC+GGG 0.441892 4_1:+384548 None:intergenic
CTCAAAGGGCTCAATGCTAA+TGG 0.444928 4_1:+383834 None:intergenic
CTCAAAGGACTCAATGGAAA+TGG 0.446507 4_1:+383900 None:intergenic
TTCCCAATCTTCTCCTCACT+TGG 0.447928 4_1:+385799 None:intergenic
ATTCCTACTATAGATAATAA+GGG 0.450157 4_1:-384730 Msa0527760:CDS
TGAAAATGATTATCCTCAAA+AGG 0.452553 4_1:+385144 None:intergenic
GCAAGCAATTGGAGAATGCT+TGG 0.459665 4_1:-386022 Msa0527760:CDS
GAGAACAAAATCTGTCTCCT+TGG 0.464084 4_1:+385546 None:intergenic
GGAGATGTCAGGGTTGGATC+AGG 0.464317 4_1:-386159 Msa0527760:CDS
TTACCAAGTGAGGAGAAGAT+TGG 0.469923 4_1:-385802 Msa0527760:CDS
AGGAATTTAAGGAAATTCAA+TGG 0.476317 4_1:-385770 Msa0527760:CDS
ACCACCAACATTATCTGGTC+GGG 0.478543 4_1:-384366 Msa0527760:CDS
CACCGCCAATGAGTCCTTCA+AGG 0.481111 4_1:-383992 Msa0527760:CDS
AAAGCCGATGCATATAGCTT+TGG 0.483467 4_1:-385201 Msa0527760:CDS
CAGGTGCATACCAAATTATA+GGG 0.486379 4_1:+385283 None:intergenic
TCACCAACACATAATCCAAT+TGG 0.489553 4_1:-385318 Msa0527760:CDS
TACAAGGATTAAGGTTTCCA+TGG 0.498240 4_1:+385451 None:intergenic
TATGGACTCAAAGGACTCAA+TGG 0.498751 4_1:+383894 None:intergenic
TTCACCTTCTTTGAATACCC+TGG 0.500775 4_1:+386325 None:intergenic
CTACCCTTATTATCTATAGT+AGG 0.503856 4_1:+384727 None:intergenic
ACCAACCAAAATATCAAAGC+AGG 0.507591 4_1:-385111 Msa0527760:CDS
GTTCTTACATAAGGACTCAA+AGG 0.508350 4_1:+383819 None:intergenic
ATTGAAGCGATCGAGAATGC+TGG 0.508806 4_1:-386182 Msa0527760:CDS
CTTTCCAGGGTATTCAAAGA+AGG 0.511780 4_1:-386329 Msa0527760:CDS
TCTAGCTCAGTTAAAACCTC+AGG 0.514317 4_1:+385264 None:intergenic
TTGAAGCGATCGAGAATGCT+GGG 0.515187 4_1:-386181 Msa0527760:CDS
ACTGATCCGTCTCAGCGTCC+TGG 0.518950 4_1:-385021 Msa0527760:CDS
CTTCTGGTGTATGACCTTGA+AGG 0.519264 4_1:+383978 None:intergenic
AGAATGCTTGGAGAGAAGAT+AGG 0.519476 4_1:-386010 Msa0527760:CDS
AGTTGATGAACAAGGATTTG+TGG 0.524686 4_1:-385593 Msa0527760:CDS
TGCTGGGGAGATGTCAGGGT+TGG 0.526191 4_1:-386165 Msa0527760:CDS
GGAAATCCAATATTAACAAA+GGG 0.528711 4_1:-384250 Msa0527760:CDS
CCTTATGTAAGAACAACATA+TGG 0.529072 4_1:-383810 Msa0527760:CDS
CTATAACCCCAGTTAAAAGA+AGG 0.532741 4_1:-384298 Msa0527760:CDS
AGGAAATCCAATATTAACAA+AGG 0.532942 4_1:-384251 Msa0527760:CDS
ATATTATATGTCCTCTCCAC+AGG 0.535682 4_1:+384844 None:intergenic
TATGACCTTGAAGGACTCAT+TGG 0.538589 4_1:+383987 None:intergenic
CAAGAAAATATGATGTGGAA+TGG 0.539842 4_1:-386106 Msa0527760:CDS
ACACAATCGCAGATGAGATA+AGG 0.544064 4_1:-386415 Msa0527760:CDS
GTGCCGGAATGGAACAGCTC+CGG 0.546196 4_1:-386550 None:intergenic
TTTGTTCTCTATACCTGTGG+TGG 0.547282 4_1:-385532 Msa0527760:CDS
GTAGATTGTTGTGACATTGA+AGG 0.548073 4_1:-384886 Msa0527760:CDS
ATGCTTGGAGAGAAGATAGG+TGG 0.548742 4_1:-386007 Msa0527760:CDS
TATAATTTGGTATGCACCTG+AGG 0.550991 4_1:-385280 Msa0527760:CDS
TTCTTACATAAGGACTCAAA+GGG 0.551714 4_1:+383820 None:intergenic
GGTTGATCTCATGCTTCAAA+TGG 0.555778 4_1:-385511 Msa0527760:CDS
AACATATTGCAATATCTATG+TGG 0.560041 4_1:-385657 Msa0527760:CDS
ACCCTGGAAAGCTCTCTAAG+AGG 0.560256 4_1:+386341 None:intergenic
ATGGAACAGCTCCGGCACAT+TGG 0.561500 4_1:-386542 Msa0527760:CDS
AGCAATCAAAGGACTAGAAG+AGG 0.562315 4_1:-383867 Msa0527760:CDS
GGCGAGCGATATGGAAACAA+AGG 0.564210 4_1:+385081 None:intergenic
CAAGAACCTCACCAATGTGC+CGG 0.564531 4_1:+386531 None:intergenic
GTGGAGATATTCAATGCCCC+TGG 0.567677 4_1:+385488 None:intergenic
TCAATGTCACAACAATCTAC+AGG 0.568956 4_1:+384889 None:intergenic
GACCTTGAAGGACTCATTGG+CGG 0.570717 4_1:+383990 None:intergenic
TTGTTGAAGAAATTGATGAA+TGG 0.570718 4_1:-385903 Msa0527760:CDS
TCACCAATTGGATTATGTGT+TGG 0.571772 4_1:+385315 None:intergenic
GAGAAACCAGGACGCTGAGA+CGG 0.572704 4_1:+385015 None:intergenic
TACCACCAACATTATCTGGT+CGG 0.572730 4_1:-384367 Msa0527760:CDS
GTCTGAGCCAACAAAGGATG+TGG 0.574953 4_1:-384701 Msa0527760:CDS
TTGCTCACATATGGACTCAA+AGG 0.582014 4_1:+383885 None:intergenic
TCTCATGCTTCAAATGGCCA+GGG 0.583448 4_1:-385505 Msa0527760:CDS
AGTGAGGAGAAGATTGGGAA+GGG 0.587219 4_1:-385796 Msa0527760:CDS
TCGATCGCTTCAATAACCGC+AGG 0.587690 4_1:+386191 None:intergenic
GTGAGGAGAAGATTGGGAAG+GGG 0.591731 4_1:-385795 Msa0527760:CDS
GAGGCTCATAGAAGCACTCA+AGG 0.595114 4_1:-385985 Msa0527760:CDS
GTCCATATGTGAGCAATCAA+AGG 0.596259 4_1:-383878 Msa0527760:CDS
CATACCAAAGCTATATGCAT+CGG 0.599646 4_1:+385197 None:intergenic
AAAGCTAGAAACTCACAAGA+AGG 0.600879 4_1:-385417 Msa0527760:CDS
AACTTGATGTCAATGATGAC+TGG 0.601724 4_1:+384321 None:intergenic
AGAATGCTGGGGAGATGTCA+GGG 0.601811 4_1:-386169 Msa0527760:CDS
TGAGAGAGAATTGTGGTCCA+AGG 0.604253 4_1:-385563 Msa0527760:CDS
TCCTCTTAGAGAGCTTTCCA+GGG 0.606681 4_1:-386342 Msa0527760:CDS
TTTCTCTGAGTACTTACAAG+AGG 0.613541 4_1:+385221 None:intergenic
ACCCGACCAGATAATGTTGG+TGG 0.618092 4_1:+384365 None:intergenic
AGCATGAGATCAACCACCAC+AGG 0.618436 4_1:+385519 None:intergenic
CTTGGAGAGAAGATAGGTGG+AGG 0.618932 4_1:-386004 Msa0527760:CDS
AAAATATCAAAGCAGGAGAG+AGG 0.620693 4_1:-385104 Msa0527760:CDS
GAAACCCGACCAGATAATGT+TGG 0.622199 4_1:+384362 None:intergenic
CTACAAATAGATGAGAAACC+AGG 0.623694 4_1:+385003 None:intergenic
AACTATCCATATCTGAAACA+TGG 0.625924 4_1:+383949 None:intergenic
ACAGTAATGATAAGGTATTG+TGG 0.632731 4_1:-385854 Msa0527760:CDS
ATGTCACAACAATCTACAGG+TGG 0.639302 4_1:+384892 None:intergenic
ATGCTAGAGTGAATATGTCG+TGG 0.640538 4_1:+386441 None:intergenic
AGGATTGATCAAAACATACT+CGG 0.640765 4_1:+384942 None:intergenic
CAAAAGTCTCAGAATCTGAG+AGG 0.650694 4_1:-384271 Msa0527760:CDS
TCTCTAAGAGGACATTCAAG+AGG 0.651164 4_1:+386353 None:intergenic
ACAGCTCCGGCACATTGGTG+AGG 0.651948 4_1:-386537 Msa0527760:CDS
GCTTGCAAGAAAATATGATG+TGG 0.653459 4_1:-386111 Msa0527760:CDS
TAGTGAGTCTGAGCCAACAA+AGG 0.653550 4_1:-384707 Msa0527760:CDS
CGATCGCTTCAATAACCGCA+GGG 0.654532 4_1:+386192 None:intergenic
AAGTGAGGAGAAGATTGGGA+AGG 0.655591 4_1:-385797 Msa0527760:CDS
AATTGGATTATGTGTTGGTG+AGG 0.655784 4_1:+385320 None:intergenic
TGTGACGGAGTTGATGAACA+AGG 0.656381 4_1:-385601 Msa0527760:CDS
CTCATGCTTCAAATGGCCAG+GGG 0.668271 4_1:-385504 Msa0527760:CDS
GATCCAAAGATTACCAAGTG+AGG 0.673638 4_1:-385812 Msa0527760:CDS
ATGAAGCCAAAGAAAGACCA+AGG 0.681190 4_1:-384508 Msa0527760:intron
TGTAACATTCTTCTTTGACG+GGG 0.687811 4_1:+384549 None:intergenic
GTACTAACAACAGCATTGTG+AGG 0.690491 4_1:-384037 Msa0527760:CDS
GATAGTTCTTTGAAGATACG+AGG 0.692687 4_1:-383933 Msa0527760:CDS
TGTCACAACAATCTACAGGT+GGG 0.697110 4_1:+384893 None:intergenic
TGAAGCGATCGAGAATGCTG+GGG 0.704654 4_1:-386180 Msa0527760:CDS
TTTGCTTTGCTATTTCCCTG+CGG 0.707457 4_1:-386207 Msa0527760:CDS
ACATACATGAGAGAGAATTG+TGG 0.732943 4_1:-385570 Msa0527760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATATATATATATAGC+AGG + chr4_1:385887-385906 None:intergenic 10.0%
!!! AATATACATTAAGCATTGTT+TGG - chr4_1:384033-384052 Msa0527760:CDS 20.0%
!! ATAGATATTGCAATATGTTT+GGG + chr4_1:384678-384697 None:intergenic 20.0%
!! TTATAAGCAAACATTTCATA+AGG + chr4_1:385537-385556 None:intergenic 20.0%
!! CTTATAAAGTTGTTGAAAAA+GGG - chr4_1:385550-385569 Msa0527760:CDS 20.0%
!! TATTCCTACTATAGATAATA+AGG - chr4_1:385605-385624 Msa0527760:CDS 20.0%
!! ATTCCTACTATAGATAATAA+GGG - chr4_1:385606-385625 Msa0527760:CDS 20.0%
!!! TCAGTTAGAAAAAAAGTATA+TGG - chr4_1:386139-386158 Msa0527760:CDS 20.0%
! ATTGTTTGGATTCAAAAGAT+TGG - chr4_1:384047-384066 Msa0527760:CDS 25.0%
!! AATTGCTTGCAAATTTCTTT+TGG + chr4_1:384304-384323 None:intergenic 25.0%
! AAAAGAAATTTGCAAGCAAT+TGG - chr4_1:384303-384322 Msa0527760:CDS 25.0%
! TTGTTGAAGAAATTGATGAA+TGG - chr4_1:384433-384452 Msa0527760:intron 25.0%
! GAAATTGATGAATGGTAATT+CGG - chr4_1:384441-384460 Msa0527760:intron 25.0%
! TCACAAAAACAGTAATGATA+AGG - chr4_1:384474-384493 Msa0527760:intron 25.0%
! AGGAATTTAAGGAAATTCAA+TGG - chr4_1:384566-384585 Msa0527760:CDS 25.0%
! TTTAAGGAAATTCAATGGTT+AGG - chr4_1:384571-384590 Msa0527760:CDS 25.0%
! CATAGATATTGCAATATGTT+TGG + chr4_1:384679-384698 None:intergenic 25.0%
! AACATATTGCAATATCTATG+TGG - chr4_1:384679-384698 Msa0527760:CDS 25.0%
!!! TATTTTCAAGCAAAAGTTGT+TGG - chr4_1:384943-384962 Msa0527760:CDS 25.0%
! AAGAGATTAACCCTATAATT+TGG - chr4_1:385043-385062 Msa0527760:CDS 25.0%
! TGAAAATGATTATCCTCAAA+AGG + chr4_1:385195-385214 None:intergenic 25.0%
!! TTTGAGGATAATCATTTTCA+AGG - chr4_1:385195-385214 Msa0527760:CDS 25.0%
!! TTGAGGATAATCATTTTCAA+GGG - chr4_1:385196-385215 Msa0527760:CDS 25.0%
!! ATCAAACTCACAAGATATTT+TGG + chr4_1:385279-385298 None:intergenic 25.0%
! TGAGTTTGATCAAAAAATGT+TGG - chr4_1:385289-385308 Msa0527760:CDS 25.0%
! GCTTATAAAGTTGTTGAAAA+AGG - chr4_1:385549-385568 Msa0527760:CDS 25.0%
!!! ATCAGAAATTGATTTTGGAT+AGG + chr4_1:385742-385761 None:intergenic 25.0%
! ATTGTAACATTCTTCTTTGA+CGG + chr4_1:385792-385811 None:intergenic 25.0%
! CAATAAAATTAAACTGACCT+TGG + chr4_1:385848-385867 None:intergenic 25.0%
! TGTATTGAATTGATTGAAAC+AGG - chr4_1:385917-385936 Msa0527760:CDS 25.0%
! AGGAAATCCAATATTAACAA+AGG - chr4_1:386085-386104 Msa0527760:CDS 25.0%
! GGAAATCCAATATTAACAAA+GGG - chr4_1:386086-386105 Msa0527760:CDS 25.0%
! TTGATTCCCTTTGTTAATAT+TGG + chr4_1:386095-386114 None:intergenic 25.0%
!! GTTAGAAAAAAAGTATATGG+AGG - chr4_1:386142-386161 Msa0527760:CDS 25.0%
!!! CTTGTTTTCAGATTTAAACT+AGG + chr4_1:386187-386206 None:intergenic 25.0%
! TTTGTCTTTAACCTGTTAAA+TGG + chr4_1:386220-386239 None:intergenic 25.0%
TTGAAGACAGAACTACAAAA+TGG - chr4_1:383957-383976 Msa0527760:CDS 30.0%
CAAGAAAATATGATGTGGAA+TGG - chr4_1:384230-384249 Msa0527760:CDS 30.0%
!!! ATGATCCAAAACTTTTTCAG+TGG - chr4_1:384254-384273 Msa0527760:CDS 30.0%
! AATCTCCACTGAAAAAGTTT+TGG + chr4_1:384262-384281 None:intergenic 30.0%
!!! AAACTTTTTCAGTGGAGATT+TGG - chr4_1:384262-384281 Msa0527760:CDS 30.0%
GAGATTTGGCAAACAATATT+TGG - chr4_1:384276-384295 Msa0527760:CDS 30.0%
! ATTCTTTGAAGAAGTGAGTT+TGG + chr4_1:384392-384411 None:intergenic 30.0%
!! ACAGTAATGATAAGGTATTG+TGG - chr4_1:384482-384501 Msa0527760:intron 30.0%
GATCCTAACAAAACACTAAT+TGG + chr4_1:384508-384527 None:intergenic 30.0%
GTGAGAAAGTGATAACAAAT+TGG + chr4_1:384656-384675 None:intergenic 30.0%
! GTTTCCATGGTAAATGTTTT+TGG + chr4_1:384875-384894 None:intergenic 30.0%
GTAGGATATTACAAGGATTA+AGG + chr4_1:384897-384916 None:intergenic 30.0%
! GCTTTTAGTAGGATATTACA+AGG + chr4_1:384904-384923 None:intergenic 30.0%
!!! ATATGTTTTGAGTTGTTGAC+AGG - chr4_1:385162-385181 Msa0527760:CDS 30.0%
!!! TATGTTTTGAGTTGTTGACA+GGG - chr4_1:385163-385182 Msa0527760:CDS 30.0%
AGGATTGATCAAAACATACT+CGG + chr4_1:385397-385416 None:intergenic 30.0%
CTACCCTTATTATCTATAGT+AGG + chr4_1:385612-385631 None:intergenic 30.0%
TGCATCCATAATTAAAGATC+TGG - chr4_1:385713-385732 Msa0527760:CDS 30.0%
GGTTACCAGATCTTTAATTA+TGG + chr4_1:385721-385740 None:intergenic 30.0%
!! GTGTCATCAGAAATTGATTT+TGG + chr4_1:385747-385766 None:intergenic 30.0%
TTGTAACATTCTTCTTTGAC+GGG + chr4_1:385791-385810 None:intergenic 30.0%
!! GATTTTGTTGCTTGCAATTT+AGG + chr4_1:385947-385966 None:intergenic 30.0%
TTAACCTGTTAAATGGTGAT+TGG + chr4_1:386213-386232 None:intergenic 30.0%
! AACTATCCATATCTGAAACA+TGG + chr4_1:386390-386409 None:intergenic 30.0%
TTCTTACATAAGGACTCAAA+GGG + chr4_1:386519-386538 None:intergenic 30.0%
CCATATGTTGTTCTTACATA+AGG + chr4_1:386529-386548 None:intergenic 30.0%
CCTTATGTAAGAACAACATA+TGG - chr4_1:386526-386545 Msa0527760:CDS 30.0%
! TTGGATTCAAAAGATTGGTC+TGG - chr4_1:384052-384071 Msa0527760:CDS 35.0%
!! TGGATTCAAAAGATTGGTCT+GGG - chr4_1:384053-384072 Msa0527760:CDS 35.0%
GCTTGCAAGAAAATATGATG+TGG - chr4_1:384225-384244 Msa0527760:CDS 35.0%
!! TGGCCAATTAGTGTTTTGTT+AGG - chr4_1:384502-384521 Msa0527760:intron 35.0%
! GAAAGAGTTTTCTCTTGTGA+CGG - chr4_1:384720-384739 Msa0527760:CDS 35.0%
!! AGTTGATGAACAAGGATTTG+TGG - chr4_1:384743-384762 Msa0527760:CDS 35.0%
ACATACATGAGAGAGAATTG+TGG - chr4_1:384766-384785 Msa0527760:CDS 35.0%
! GATTTTGTTCTCTATACCTG+TGG - chr4_1:384801-384820 Msa0527760:CDS 35.0%
!!! CATGGTAAATGTTTTTGGAG+TGG + chr4_1:384870-384889 None:intergenic 35.0%
CACTCCAAAAACATTTACCA+TGG - chr4_1:384868-384887 Msa0527760:CDS 35.0%
TACAAGGATTAAGGTTTCCA+TGG + chr4_1:384888-384907 None:intergenic 35.0%
!!! GTGAGTTTCTAGCTTTTAGT+AGG + chr4_1:384915-384934 None:intergenic 35.0%
AAAGCTAGAAACTCACAAGA+AGG - chr4_1:384919-384938 Msa0527760:CDS 35.0%
CAAGCAAAAGTTGTTGGATT+TGG - chr4_1:384949-384968 Msa0527760:CDS 35.0%
GGATTGTCATCTGTTGAAAA+TGG - chr4_1:384970-384989 Msa0527760:CDS 35.0%
! AATTGGATTATGTGTTGGTG+AGG + chr4_1:385019-385038 None:intergenic 35.0%
TCACCAACACATAATCCAAT+TGG - chr4_1:385018-385037 Msa0527760:CDS 35.0%
TCACCAATTGGATTATGTGT+TGG + chr4_1:385024-385043 None:intergenic 35.0%
CAGGTGCATACCAAATTATA+GGG + chr4_1:385056-385075 None:intergenic 35.0%
TCAGGTGCATACCAAATTAT+AGG + chr4_1:385057-385076 None:intergenic 35.0%
TATAATTTGGTATGCACCTG+AGG - chr4_1:385056-385075 Msa0527760:CDS 35.0%
TTTCTCTGAGTACTTACAAG+AGG + chr4_1:385118-385137 None:intergenic 35.0%
CATACCAAAGCTATATGCAT+CGG + chr4_1:385142-385161 None:intergenic 35.0%
ACCAACCAAAATATCAAAGC+AGG - chr4_1:385225-385244 Msa0527760:CDS 35.0%
!!! TCCTGCTTTGATATTTTGGT+TGG + chr4_1:385229-385248 None:intergenic 35.0%
! TCTCTCCTGCTTTGATATTT+TGG + chr4_1:385233-385252 None:intergenic 35.0%
AAAATATCAAAGCAGGAGAG+AGG - chr4_1:385232-385251 Msa0527760:CDS 35.0%
CTACAAATAGATGAGAAACC+AGG + chr4_1:385336-385355 None:intergenic 35.0%
CTGGTTTCTCATCTATTTGT+AGG - chr4_1:385334-385353 Msa0527760:CDS 35.0%
! AGTTCAAGCTGATTTAGTTC+AGG + chr4_1:385417-385436 None:intergenic 35.0%
TCAATGTCACAACAATCTAC+AGG + chr4_1:385450-385469 None:intergenic 35.0%
GTAGATTGTTGTGACATTGA+AGG - chr4_1:385450-385469 Msa0527760:CDS 35.0%
! TTTTCTCAAGAGTTTTCCTG+TGG - chr4_1:385476-385495 Msa0527760:CDS 35.0%
ATATTATATGTCCTCTCCAC+AGG + chr4_1:385495-385514 None:intergenic 35.0%
TGTAACATTCTTCTTTGACG+GGG + chr4_1:385790-385809 None:intergenic 35.0%
! TTCTTTGGCTTCATTTGTGA+TGG + chr4_1:385822-385841 None:intergenic 35.0%
TCATTACCACCAACATTATC+TGG - chr4_1:385965-385984 Msa0527760:CDS 35.0%
AACTTGATGTCAATGATGAC+TGG + chr4_1:386018-386037 None:intergenic 35.0%
CTATAACCCCAGTTAAAAGA+AGG - chr4_1:386038-386057 Msa0527760:CDS 35.0%
! TTGACACGTCTTATTCTTGT+TGG + chr4_1:386277-386296 None:intergenic 35.0%
! GATAGTTCTTTGAAGATACG+AGG - chr4_1:386403-386422 Msa0527760:CDS 35.0%
GTTCTTACATAAGGACTCAA+AGG + chr4_1:386520-386539 None:intergenic 35.0%
!! TGGAAGTCTAAAAGCGCTTA+TGG - chr4_1:383826-383845 Msa0527760:CDS 40.0%
ATGCTAGAGTGAATATGTCG+TGG + chr4_1:383898-383917 None:intergenic 40.0%
ACACAATCGCAGATGAGATA+AGG - chr4_1:383921-383940 Msa0527760:CDS 40.0%
! TCTCTAAGAGGACATTCAAG+AGG + chr4_1:383986-384005 None:intergenic 40.0%
! CTTTCCAGGGTATTCAAAGA+AGG - chr4_1:384007-384026 Msa0527760:CDS 40.0%
TTCACCTTCTTTGAATACCC+TGG + chr4_1:384014-384033 None:intergenic 40.0%
!! TTTGCTTTGCTATTTCCCTG+CGG - chr4_1:384129-384148 Msa0527760:CDS 40.0%
AGAATGCTTGGAGAGAAGAT+AGG - chr4_1:384326-384345 Msa0527760:CDS 40.0%
! GAAGCACTCAAGGAGAAAAA+AGG - chr4_1:384361-384380 Msa0527760:CDS 40.0%
GATCCAAAGATTACCAAGTG+AGG - chr4_1:384524-384543 Msa0527760:CDS 40.0%
! TCTCCTCACTTGGTAATCTT+TGG + chr4_1:384530-384549 None:intergenic 40.0%
TTACCAAGTGAGGAGAAGAT+TGG - chr4_1:384534-384553 Msa0527760:CDS 40.0%
TACCAAGTGAGGAGAAGATT+GGG - chr4_1:384535-384554 Msa0527760:CDS 40.0%
! TAGGACAAAGTTTTGCTCTG+AGG - chr4_1:384590-384609 Msa0527760:CDS 40.0%
!!! TTTGCTCTGAGGCATTTTCT+TGG - chr4_1:384601-384620 Msa0527760:CDS 40.0%
!!! TTGCTCTGAGGCATTTTCTT+GGG - chr4_1:384602-384621 Msa0527760:CDS 40.0%
GAGAACAAAATCTGTCTCCT+TGG + chr4_1:384793-384812 None:intergenic 40.0%
TTTGTTCTCTATACCTGTGG+TGG - chr4_1:384804-384823 Msa0527760:CDS 40.0%
GGTTGATCTCATGCTTCAAA+TGG - chr4_1:384825-384844 Msa0527760:CDS 40.0%
GGGTTAATCTCTTCACCAAT+TGG + chr4_1:385036-385055 None:intergenic 40.0%
! TCTAGCTCAGTTAAAACCTC+AGG + chr4_1:385075-385094 None:intergenic 40.0%
AAAGCCGATGCATATAGCTT+TGG - chr4_1:385135-385154 Msa0527760:CDS 40.0%
! GACAGGGAAAATTCCTTTTG+AGG - chr4_1:385179-385198 Msa0527760:CDS 40.0%
!!! AGATATTTTGGCGAGCGATA+TGG + chr4_1:385267-385286 None:intergenic 40.0%
TGTCACAACAATCTACAGGT+GGG + chr4_1:385446-385465 None:intergenic 40.0%
ATGTCACAACAATCTACAGG+TGG + chr4_1:385447-385466 None:intergenic 40.0%
CATCTTCACAAACTGTGCTT+TGG + chr4_1:385689-385708 None:intergenic 40.0%
ATGAAGCCAAAGAAAGACCA+AGG - chr4_1:385828-385847 Msa0527760:CDS 40.0%
! AACTGACCTTGGTCTTTCTT+TGG + chr4_1:385837-385856 None:intergenic 40.0%
TACCACCAACATTATCTGGT+CGG - chr4_1:385969-385988 Msa0527760:CDS 40.0%
TCGGGTTTCAAGGACAAATA+AGG - chr4_1:385988-386007 Msa0527760:CDS 40.0%
!! TTGGAGGCCTTCTTTTAACT+GGG + chr4_1:386048-386067 None:intergenic 40.0%
! TTTGGAGGCCTTCTTTTAAC+TGG + chr4_1:386049-386068 None:intergenic 40.0%
!!! TCAGATTCTGAGACTTTTGG+AGG + chr4_1:386064-386083 None:intergenic 40.0%
!!! CTCTCAGATTCTGAGACTTT+TGG + chr4_1:386067-386086 None:intergenic 40.0%
CAAAAGTCTCAGAATCTGAG+AGG - chr4_1:386065-386084 Msa0527760:CDS 40.0%
GAGACCAATCACCATTTAAC+AGG - chr4_1:386206-386225 Msa0527760:CDS 40.0%
GTACTAACAACAGCATTGTG+AGG - chr4_1:386299-386318 Msa0527760:CDS 40.0%
AGCATTGTGAGGATGCAAAA+AGG - chr4_1:386310-386329 Msa0527760:CDS 40.0%
GCATTGTGAGGATGCAAAAA+GGG - chr4_1:386311-386330 Msa0527760:CDS 40.0%
TATGACCTTGAAGGACTCAT+TGG + chr4_1:386352-386371 None:intergenic 40.0%
ATGCTGCCATGTTTCAGATA+TGG - chr4_1:386381-386400 Msa0527760:CDS 40.0%
CTCAAAGGACTCAATGGAAA+TGG + chr4_1:386439-386458 None:intergenic 40.0%
TATGGACTCAAAGGACTCAA+TGG + chr4_1:386445-386464 None:intergenic 40.0%
TTGCTCACATATGGACTCAA+AGG + chr4_1:386454-386473 None:intergenic 40.0%
GTCCATATGTGAGCAATCAA+AGG - chr4_1:386458-386477 Msa0527760:CDS 40.0%
GTCCTTTGATTGCTCACATA+TGG + chr4_1:386463-386482 None:intergenic 40.0%
AGCAATCAAAGGACTAGAAG+AGG - chr4_1:386469-386488 Msa0527760:CDS 40.0%
TCCTCTTAGAGAGCTTTCCA+GGG - chr4_1:383994-384013 Msa0527760:CDS 45.0%
ATTGAAGCGATCGAGAATGC+TGG - chr4_1:384154-384173 Msa0527760:CDS 45.0%
TTGAAGCGATCGAGAATGCT+GGG - chr4_1:384155-384174 Msa0527760:CDS 45.0%
GCAAGCAATTGGAGAATGCT+TGG - chr4_1:384314-384333 Msa0527760:CDS 45.0%
ATGCTTGGAGAGAAGATAGG+TGG - chr4_1:384329-384348 Msa0527760:CDS 45.0%
TTCCCAATCTTCTCCTCACT+TGG + chr4_1:384540-384559 None:intergenic 45.0%
AAGTGAGGAGAAGATTGGGA+AGG - chr4_1:384539-384558 Msa0527760:CDS 45.0%
AGTGAGGAGAAGATTGGGAA+GGG - chr4_1:384540-384559 Msa0527760:CDS 45.0%
!!! TGCTCTGAGGCATTTTCTTG+GGG - chr4_1:384603-384622 Msa0527760:CDS 45.0%
!! TGTGACGGAGTTGATGAACA+AGG - chr4_1:384735-384754 Msa0527760:CDS 45.0%
TGAGAGAGAATTGTGGTCCA+AGG - chr4_1:384773-384792 Msa0527760:CDS 45.0%
ATCTCATGCTTCAAATGGCC+AGG - chr4_1:384830-384849 Msa0527760:CDS 45.0%
TCTCATGCTTCAAATGGCCA+GGG - chr4_1:384831-384850 Msa0527760:CDS 45.0%
! TCAAGAGTTTTCCTGTGGAG+AGG - chr4_1:385481-385500 Msa0527760:CDS 45.0%
TAGTGAGTCTGAGCCAACAA+AGG - chr4_1:385629-385648 Msa0527760:CDS 45.0%
ACAGTAGCCACATCCTTTGT+TGG + chr4_1:385645-385664 None:intergenic 45.0%
ACCACCAACATTATCTGGTC+GGG - chr4_1:385970-385989 Msa0527760:CDS 45.0%
GAAACCCGACCAGATAATGT+TGG + chr4_1:385977-385996 None:intergenic 45.0%
CATTATCTGGTCGGGTTTCA+AGG - chr4_1:385978-385997 Msa0527760:CDS 45.0%
!! TGGAGGCCTTCTTTTAACTG+GGG + chr4_1:386047-386066 None:intergenic 45.0%
CTTCTGGTGTATGACCTTGA+AGG + chr4_1:386361-386380 None:intergenic 45.0%
TGAAACATGGCAGCATCTTC+TGG + chr4_1:386377-386396 None:intergenic 45.0%
CTCAAAGGGCTCAATGCTAA+TGG + chr4_1:386505-386524 None:intergenic 45.0%
CAAGAACCTCACCAATGTGC+CGG + chr4_1:383808-383827 None:intergenic 50.0%
GTCCTCTTAGAGAGCTTTCC+AGG - chr4_1:383993-384012 Msa0527760:CDS 50.0%
ACCCTGGAAAGCTCTCTAAG+AGG + chr4_1:383998-384017 None:intergenic 50.0%
CGATCGCTTCAATAACCGCA+GGG + chr4_1:384147-384166 None:intergenic 50.0%
TCGATCGCTTCAATAACCGC+AGG + chr4_1:384148-384167 None:intergenic 50.0%
TGAAGCGATCGAGAATGCTG+GGG - chr4_1:384156-384175 Msa0527760:CDS 50.0%
AGAATGCTGGGGAGATGTCA+GGG - chr4_1:384167-384186 Msa0527760:CDS 50.0%
CTTGGAGAGAAGATAGGTGG+AGG - chr4_1:384332-384351 Msa0527760:CDS 50.0%
!! GAGGCTCATAGAAGCACTCA+AGG - chr4_1:384351-384370 Msa0527760:CDS 50.0%
GTGAGGAGAAGATTGGGAAG+GGG - chr4_1:384541-384560 Msa0527760:CDS 50.0%
TGAGGAGAAGATTGGGAAGG+GGG - chr4_1:384542-384561 Msa0527760:CDS 50.0%
GGGAAGGGGGAAGGAATTTA+AGG - chr4_1:384555-384574 Msa0527760:CDS 50.0%
AGCATGAGATCAACCACCAC+AGG + chr4_1:384820-384839 None:intergenic 50.0%
CTCATGCTTCAAATGGCCAG+GGG - chr4_1:384832-384851 Msa0527760:CDS 50.0%
GTGGAGATATTCAATGCCCC+TGG + chr4_1:384851-384870 None:intergenic 50.0%
GGCGAGCGATATGGAAACAA+AGG + chr4_1:385258-385277 None:intergenic 50.0%
GTCTGAGCCAACAAAGGATG+TGG - chr4_1:385635-385654 Msa0527760:CDS 50.0%
ACCCGACCAGATAATGTTGG+TGG + chr4_1:385974-385993 None:intergenic 50.0%
GACCTTGAAGGACTCATTGG+CGG + chr4_1:386349-386368 None:intergenic 50.0%
ATGGAACAGCTCCGGCACAT+TGG - chr4_1:383794-383813 Msa0527760:CDS 55.0%
CGGCACATTGGTGAGGTTCT+TGG - chr4_1:383806-383825 Msa0527760:CDS 55.0%
GAGAATGCTGGGGAGATGTC+AGG - chr4_1:384166-384185 Msa0527760:CDS 55.0%
GGAGATGTCAGGGTTGGATC+AGG - chr4_1:384177-384196 Msa0527760:CDS 55.0%
GAGAAGATTGGGAAGGGGGA+AGG - chr4_1:384546-384565 Msa0527760:CDS 55.0%
GAGAAACCAGGACGCTGAGA+CGG + chr4_1:385324-385343 None:intergenic 55.0%
CACCGCCAATGAGTCCTTCA+AGG - chr4_1:386344-386363 Msa0527760:CDS 55.0%
ACAGCTCCGGCACATTGGTG+AGG - chr4_1:383799-383818 Msa0527760:CDS 60.0%
TGCTGGGGAGATGTCAGGGT+TGG - chr4_1:384171-384190 Msa0527760:CDS 60.0%
ACTGATCCGTCTCAGCGTCC+TGG - chr4_1:385315-385334 Msa0527760:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 383794 386564 383794 ID=Msa0527760;Name=Msa0527760
chr4_1 mRNA 383794 386564 383794 ID=Msa0527760-mRNA-1;Parent=Msa0527760;Name=Msa0527760-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|893
chr4_1 exon 384509 386564 384509 ID=Msa0527760-mRNA-1:exon:24;Parent=Msa0527760-mRNA-1
chr4_1 exon 383794 384419 383794 ID=Msa0527760-mRNA-1:exon:23;Parent=Msa0527760-mRNA-1
chr4_1 CDS 384509 386564 384509 ID=Msa0527760-mRNA-1:cds;Parent=Msa0527760-mRNA-1
chr4_1 CDS 383794 384419 383794 ID=Msa0527760-mRNA-1:cds;Parent=Msa0527760-mRNA-1
Gene Sequence

>Msa0527760

ATGGAACAGCTCCGGCACATTGGTGAGGTTCTTGGAAGTCTAAAAGCGCTTATGGTTTTAAGCGACGAAATTCAAATCAATCAACGACAATGTTGTTTGCTCCACGACATATTCACTCTAGCATTCGACACAATCGCAGATGAGATAAGGCAGAATCTGAAGCTTGAAGACAGAACTACAAAATGGAAGCCTCTTGAATGTCCTCTTAGAGAGCTTTCCAGGGTATTCAAAGAAGGTGAAATATACATTAAGCATTGTTTGGATTCAAAAGATTGGTCTGGGAAAGCACTCACACTTTCTCAGAACAGAGAATGTGTTGAGTTTCACATTCATAATTTGCTTTGCTATTTCCCTGCGGTTATTGAAGCGATCGAGAATGCTGGGGAGATGTCAGGGTTGGATCAGGATGAGAATTCGAAGAAGAAATTGATGCTTGCAAGAAAATATGATGTGGAATGGAATGATCCAAAACTTTTTCAGTGGAGATTTGGCAAACAATATTTGGTTCCAAAAGAAATTTGCAAGCAATTGGAGAATGCTTGGAGAGAAGATAGGTGGAGGCTCATAGAAGCACTCAAGGAGAAAAAAGGTTCTTCCAAACTCACTTCTTCAAAGAATGATCAACATTTTGCAGATATGTTGTTGAAGAAATTGATGAATGGTAATTCGGATAAAACTAATCACAAAAACAGTAATGATAAGGTATTGTGGCCAATTAGTGTTTTGTTAGGATCCAAAGATTACCAAGTGAGGAGAAGATTGGGAAGGGGGAAGGAATTTAAGGAAATTCAATGGTTAGGACAAAGTTTTGCTCTGAGGCATTTTCTTGGGGAGAGAGAAGCTTATGAAAATGAGATTTCCAATTTGTTATCACTTTCTCACCCAAACATATTGCAATATCTATGTGGTTTTTATGATGAAGAGAAGAAAGAGTTTTCTCTTGTGACGGAGTTGATGAACAAGGATTTGTGGACATACATGAGAGAGAATTGTGGTCCAAGGAGACAGATTTTGTTCTCTATACCTGTGGTGGTTGATCTCATGCTTCAAATGGCCAGGGGCATTGAATATCTCCACTCCAAAAACATTTACCATGGAAACCTTAATCCTTGTAATATCCTACTAAAAGCTAGAAACTCACAAGAAGGTTATTTTCAAGCAAAAGTTGTTGGATTTGGATTGTCATCTGTTGAAAATGGTGAAATTTATAATGCTTCTAGATCCTCACCAACACATAATCCAATTGGTGAAGAGATTAACCCTATAATTTGGTATGCACCTGAGGTTTTAACTGAGCTAGAACAAATGAAAAATGCTTCTACCTCTTGTAAGTACTCAGAGAAAGCCGATGCATATAGCTTTGGTATGATATGTTTTGAGTTGTTGACAGGGAAAATTCCTTTTGAGGATAATCATTTTCAAGGGATTAGAACCAACCAAAATATCAAAGCAGGAGAGAGGCCTTTGTTTCCATATCGCTCGCCAAAATATCTTGTGAGTTTGATCAAAAAATGTTGGCAAACTGATCCGTCTCAGCGTCCTGGTTTCTCATCTATTTGTAGGATTTTGCGCTACATCAAAAAGTTTCTTTCAATGAACACCGAGTATGTTTTGATCAATCCTGAACTAAATCAGCTTGAACTTCAGAACCCACCTGTAGATTGTTGTGACATTGAAGGAATTTTTCTCAAGAGTTTTCCTGTGGAGAGGACATATAATATGTCTTGTGTTTCACAAATACCTTATGAAATGTTTGCTTATAAAGTTGTTGAAAAAGGGAAGATTAATAATCAGAATCTGAGTAATAATACTATTCCTACTATAGATAATAAGGGTAGTGAGTCTGAGCCAACAAAGGATGTGGCTACTGTTCGTAGCGAAGAAAGTGATGACCAAAGCACAGTTTGTGAAGATGTTGATGCATCCATAATTAAAGATCTGGTAACCTATCCAAAATCAATTTCTGATGACACACAGTCTATTTTTTATGATACCCCGTCAAAGAAGAATGTTACAATAAAGAAACCATCACAAATGAAGCCAAAGAAAGACCAAGGAACGCCTAAATTGCAAGCAACAAAATCATTACCACCAACATTATCTGGTCGGGTTTCAAGGACAAATAAGGCGAACCAGTCATCATTGACATCAAGTTCTATAACCCCAGTTAAAAGAAGGCCTCCAAAAGTCTCAGAATCTGAGAGGAAATCCAATATTAACAAAGGGAATCAATCAGCATCAGTGTCAACTTCAAGCTCAGTTAGAAAAAAAGTATATGGAGGCAATGTCTCAGATTCTAAGCCTAGTTTAAATCTGAAAACAAGAGACCAATCACCATTTAACAGGTTAAAGACAAAAAAATCACAACTAACGATGAATTCTTCTTCGAGTCCAACAAGAATAAGACGTGTCAATAGTACTAACAACAGCATTGTGAGGATGCAAAAAGGGTTCATTTCAACACCGCCAATGAGTCCTTCAAGGTCATACACCAGAAGATGCTGCCATGTTTCAGATATGGATAGTTCTTTGAAGATACGAGGTAGATTGTCACCATTTCCATTGAGTCCTTTGAGTCCATATGTGAGCAATCAAAGGACTAGAAGAGGAAGCTTGTCACCATTAGCATTGAGCCCTTTGAGTCCTTATGTAAGAACAACATATGGTCATGTCTCAGATTAA
Protein sequence

>Msa0527760

MEQLRHIGEVLGSLKALMVLSDEIQINQRQCCLLHDIFTLAFDTIADEIRQNLKLEDRTTKWKPLECPLRELSRVFKEGEIYIKHCLDSKDWSGKALTLSQNRECVEFHIHNLLCYFPAVIEAIENAGEMSGLDQDENSKKKLMLARKYDVEWNDPKLFQWRFGKQYLVPKEICKQLENAWREDRWRLIEALKEKKGSSKLTSSKNDQHFADMLLKKLMNGNSDKTNHKNSNDKVLWPISVLLGSKDYQVRRRLGRGKEFKEIQWLGQSFALRHFLGEREAYENEISNLLSLSHPNILQYLCGFYDEEKKEFSLVTELMNKDLWTYMRENCGPRRQILFSIPVVVDLMLQMARGIEYLHSKNIYHGNLNPCNILLKARNSQEGYFQAKVVGFGLSSVENGEIYNASRSSPTHNPIGEEINPIIWYAPEVLTELEQMKNASTSCKYSEKADAYSFGMICFELLTGKIPFEDNHFQGIRTNQNIKAGERPLFPYRSPKYLVSLIKKCWQTDPSQRPGFSSICRILRYIKKFLSMNTEYVLINPELNQLELQNPPVDCCDIEGIFLKSFPVERTYNMSCVSQIPYEMFAYKVVEKGKINNQNLSNNTIPTIDNKGSESEPTKDVATVRSEESDDQSTVCEDVDASIIKDLVTYPKSISDDTQSIFYDTPSKKNVTIKKPSQMKPKKDQGTPKLQATKSLPPTLSGRVSRTNKANQSSLTSSSITPVKRRPPKVSESERKSNINKGNQSASVSTSSSVRKKVYGGNVSDSKPSLNLKTRDQSPFNRLKTKKSQLTMNSSSSPTRIRRVNSTNNSIVRMQKGFISTPPMSPSRSYTRRCCHVSDMDSSLKIRGRLSPFPLSPLSPYVSNQRTRRGSLSPLALSPLSPYVRTTYGHVSD*