Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0527760 | RHN53538.1 | 97.312 | 893 | 24 | 0 | 1 | 893 | 25 | 917 | 0.0 | 1804 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0527760 | sp|P25848|PHY1_CERPU | 35.117 | 299 | 163 | 12 | 249 | 530 | 1005 | 1289 | 2.04e-35 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0527760 | A0A396HLT5 | 97.312 | 893 | 24 | 0 | 1 | 893 | 25 | 917 | 0.0 | 1804 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0527760 | MtrunA17_Chr5g0396901 | 97.312 | 893 | 24 | 0 | 1 | 893 | 25 | 917 | 0.0 | 1804 |
| Msa0527760 | MtrunA17_Chr8g0347481 | 53.333 | 600 | 246 | 8 | 1 | 595 | 1 | 571 | 0.0 | 635 |
| Msa0527760 | MtrunA17_Chr4g0042731 | 29.773 | 309 | 168 | 15 | 247 | 539 | 142 | 417 | 8.22e-25 | 108 |
| Msa0527760 | MtrunA17_Chr4g0075541 | 31.474 | 251 | 130 | 9 | 280 | 525 | 131 | 344 | 1.27e-24 | 107 |
| Msa0527760 | MtrunA17_Chr4g0059121 | 29.845 | 258 | 142 | 10 | 282 | 536 | 104 | 325 | 1.18e-23 | 103 |
| Msa0527760 | MtrunA17_Chr4g0065211 | 30.556 | 288 | 150 | 11 | 264 | 535 | 1139 | 1392 | 2.16e-23 | 107 |
| Msa0527760 | MtrunA17_Chr3g0117731 | 30.233 | 258 | 141 | 9 | 282 | 536 | 103 | 324 | 2.22e-23 | 102 |
| Msa0527760 | MtrunA17_Chr5g0406251 | 26.104 | 249 | 146 | 8 | 282 | 528 | 337 | 549 | 5.12e-23 | 104 |
| Msa0527760 | MtrunA17_Chr4g0047191 | 27.273 | 253 | 145 | 10 | 282 | 531 | 92 | 308 | 3.10e-22 | 99.4 |
| Msa0527760 | MtrunA17_Chr4g0047211 | 27.273 | 253 | 145 | 10 | 282 | 531 | 87 | 303 | 3.85e-22 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0527760 | AT1G64300.2 | 52.510 | 777 | 297 | 14 | 1 | 767 | 1 | 715 | 0.0 | 785 |
| Msa0527760 | AT1G64300.1 | 52.510 | 777 | 297 | 14 | 1 | 767 | 1 | 715 | 0.0 | 785 |
| Msa0527760 | AT5G41730.2 | 52.291 | 742 | 295 | 11 | 1 | 726 | 1 | 699 | 0.0 | 764 |
| Msa0527760 | AT5G41730.1 | 52.291 | 742 | 295 | 11 | 1 | 726 | 1 | 699 | 0.0 | 764 |
| Msa0527760 | AT1G01450.1 | 53.590 | 390 | 151 | 7 | 211 | 594 | 2 | 367 | 1.45e-131 | 403 |
| Msa0527760 | AT1G14000.1 | 31.392 | 309 | 165 | 17 | 247 | 540 | 161 | 437 | 1.82e-24 | 107 |
| Msa0527760 | AT1G62400.1 | 30.645 | 248 | 131 | 8 | 280 | 523 | 130 | 340 | 5.47e-24 | 105 |
| Msa0527760 | AT3G46930.2 | 25.959 | 339 | 198 | 10 | 224 | 545 | 179 | 481 | 7.60e-24 | 107 |
| Msa0527760 | AT3G46930.1 | 25.959 | 339 | 198 | 10 | 224 | 545 | 144 | 446 | 1.02e-23 | 106 |
| Msa0527760 | AT3G46920.2 | 28.378 | 370 | 191 | 16 | 192 | 530 | 816 | 1142 | 1.09e-22 | 105 |
Find 163 sgRNAs with CRISPR-Local
Find 205 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAATTTGCAAGCAAT+TGG | 0.319977 | 4_1:-386033 | Msa0527760:CDS |
| AAAATATCAAAGCAGGAGAG+AGG | 0.620693 | 4_1:-385104 | Msa0527760:CDS |
| AAAGCCGATGCATATAGCTT+TGG | 0.483467 | 4_1:-385201 | Msa0527760:CDS |
| AAAGCTAGAAACTCACAAGA+AGG | 0.600879 | 4_1:-385417 | Msa0527760:CDS |
| AACATATTGCAATATCTATG+TGG | 0.560041 | 4_1:-385657 | Msa0527760:CDS |
| AACTATCCATATCTGAAACA+TGG | 0.625924 | 4_1:+383949 | None:intergenic |
| AACTGACCTTGGTCTTTCTT+TGG | 0.265277 | 4_1:+384502 | None:intergenic |
| AACTTGATGTCAATGATGAC+TGG | 0.601724 | 4_1:+384321 | None:intergenic |
| AAGAGATTAACCCTATAATT+TGG | 0.257480 | 4_1:-385293 | Msa0527760:CDS |
| AAGTGAGGAGAAGATTGGGA+AGG | 0.655591 | 4_1:-385797 | Msa0527760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGAAGACAGAACTACAAAA+TGG | - | chr4_1:383957-383976 | Msa0527760:CDS | 30.0% | |
| CAAGAAAATATGATGTGGAA+TGG | - | chr4_1:384230-384249 | Msa0527760:CDS | 30.0% | |
| GAGATTTGGCAAACAATATT+TGG | - | chr4_1:384276-384295 | Msa0527760:CDS | 30.0% | |
| GATCCTAACAAAACACTAAT+TGG | + | chr4_1:384508-384527 | None:intergenic | 30.0% | |
| GTGAGAAAGTGATAACAAAT+TGG | + | chr4_1:384656-384675 | None:intergenic | 30.0% | |
| GTAGGATATTACAAGGATTA+AGG | + | chr4_1:384897-384916 | None:intergenic | 30.0% | |
| AGGATTGATCAAAACATACT+CGG | + | chr4_1:385397-385416 | None:intergenic | 30.0% | |
| CTACCCTTATTATCTATAGT+AGG | + | chr4_1:385612-385631 | None:intergenic | 30.0% | |
| TGCATCCATAATTAAAGATC+TGG | - | chr4_1:385713-385732 | Msa0527760:CDS | 30.0% | |
| GGTTACCAGATCTTTAATTA+TGG | + | chr4_1:385721-385740 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 383794 | 386564 | 383794 | ID=Msa0527760;Name=Msa0527760 |
| chr4_1 | mRNA | 383794 | 386564 | 383794 | ID=Msa0527760-mRNA-1;Parent=Msa0527760;Name=Msa0527760-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|893 |
| chr4_1 | exon | 384509 | 386564 | 384509 | ID=Msa0527760-mRNA-1:exon:24;Parent=Msa0527760-mRNA-1 |
| chr4_1 | exon | 383794 | 384419 | 383794 | ID=Msa0527760-mRNA-1:exon:23;Parent=Msa0527760-mRNA-1 |
| chr4_1 | CDS | 384509 | 386564 | 384509 | ID=Msa0527760-mRNA-1:cds;Parent=Msa0527760-mRNA-1 |
| chr4_1 | CDS | 383794 | 384419 | 383794 | ID=Msa0527760-mRNA-1:cds;Parent=Msa0527760-mRNA-1 |
| Gene Sequence |
>Msa0527760 ATGGAACAGCTCCGGCACATTGGTGAGGTTCTTGGAAGTCTAAAAGCGCTTATGGTTTTAAGCGACGAAATTCAAATCAATCAACGACAATGTTGTTTGCTCCACGACATATTCACTCTAGCATTCGACACAATCGCAGATGAGATAAGGCAGAATCTGAAGCTTGAAGACAGAACTACAAAATGGAAGCCTCTTGAATGTCCTCTTAGAGAGCTTTCCAGGGTATTCAAAGAAGGTGAAATATACATTAAGCATTGTTTGGATTCAAAAGATTGGTCTGGGAAAGCACTCACACTTTCTCAGAACAGAGAATGTGTTGAGTTTCACATTCATAATTTGCTTTGCTATTTCCCTGCGGTTATTGAAGCGATCGAGAATGCTGGGGAGATGTCAGGGTTGGATCAGGATGAGAATTCGAAGAAGAAATTGATGCTTGCAAGAAAATATGATGTGGAATGGAATGATCCAAAACTTTTTCAGTGGAGATTTGGCAAACAATATTTGGTTCCAAAAGAAATTTGCAAGCAATTGGAGAATGCTTGGAGAGAAGATAGGTGGAGGCTCATAGAAGCACTCAAGGAGAAAAAAGGTTCTTCCAAACTCACTTCTTCAAAGAATGATCAACATTTTGCAGATATGTTGTTGAAGAAATTGATGAATGGTAATTCGGATAAAACTAATCACAAAAACAGTAATGATAAGGTATTGTGGCCAATTAGTGTTTTGTTAGGATCCAAAGATTACCAAGTGAGGAGAAGATTGGGAAGGGGGAAGGAATTTAAGGAAATTCAATGGTTAGGACAAAGTTTTGCTCTGAGGCATTTTCTTGGGGAGAGAGAAGCTTATGAAAATGAGATTTCCAATTTGTTATCACTTTCTCACCCAAACATATTGCAATATCTATGTGGTTTTTATGATGAAGAGAAGAAAGAGTTTTCTCTTGTGACGGAGTTGATGAACAAGGATTTGTGGACATACATGAGAGAGAATTGTGGTCCAAGGAGACAGATTTTGTTCTCTATACCTGTGGTGGTTGATCTCATGCTTCAAATGGCCAGGGGCATTGAATATCTCCACTCCAAAAACATTTACCATGGAAACCTTAATCCTTGTAATATCCTACTAAAAGCTAGAAACTCACAAGAAGGTTATTTTCAAGCAAAAGTTGTTGGATTTGGATTGTCATCTGTTGAAAATGGTGAAATTTATAATGCTTCTAGATCCTCACCAACACATAATCCAATTGGTGAAGAGATTAACCCTATAATTTGGTATGCACCTGAGGTTTTAACTGAGCTAGAACAAATGAAAAATGCTTCTACCTCTTGTAAGTACTCAGAGAAAGCCGATGCATATAGCTTTGGTATGATATGTTTTGAGTTGTTGACAGGGAAAATTCCTTTTGAGGATAATCATTTTCAAGGGATTAGAACCAACCAAAATATCAAAGCAGGAGAGAGGCCTTTGTTTCCATATCGCTCGCCAAAATATCTTGTGAGTTTGATCAAAAAATGTTGGCAAACTGATCCGTCTCAGCGTCCTGGTTTCTCATCTATTTGTAGGATTTTGCGCTACATCAAAAAGTTTCTTTCAATGAACACCGAGTATGTTTTGATCAATCCTGAACTAAATCAGCTTGAACTTCAGAACCCACCTGTAGATTGTTGTGACATTGAAGGAATTTTTCTCAAGAGTTTTCCTGTGGAGAGGACATATAATATGTCTTGTGTTTCACAAATACCTTATGAAATGTTTGCTTATAAAGTTGTTGAAAAAGGGAAGATTAATAATCAGAATCTGAGTAATAATACTATTCCTACTATAGATAATAAGGGTAGTGAGTCTGAGCCAACAAAGGATGTGGCTACTGTTCGTAGCGAAGAAAGTGATGACCAAAGCACAGTTTGTGAAGATGTTGATGCATCCATAATTAAAGATCTGGTAACCTATCCAAAATCAATTTCTGATGACACACAGTCTATTTTTTATGATACCCCGTCAAAGAAGAATGTTACAATAAAGAAACCATCACAAATGAAGCCAAAGAAAGACCAAGGAACGCCTAAATTGCAAGCAACAAAATCATTACCACCAACATTATCTGGTCGGGTTTCAAGGACAAATAAGGCGAACCAGTCATCATTGACATCAAGTTCTATAACCCCAGTTAAAAGAAGGCCTCCAAAAGTCTCAGAATCTGAGAGGAAATCCAATATTAACAAAGGGAATCAATCAGCATCAGTGTCAACTTCAAGCTCAGTTAGAAAAAAAGTATATGGAGGCAATGTCTCAGATTCTAAGCCTAGTTTAAATCTGAAAACAAGAGACCAATCACCATTTAACAGGTTAAAGACAAAAAAATCACAACTAACGATGAATTCTTCTTCGAGTCCAACAAGAATAAGACGTGTCAATAGTACTAACAACAGCATTGTGAGGATGCAAAAAGGGTTCATTTCAACACCGCCAATGAGTCCTTCAAGGTCATACACCAGAAGATGCTGCCATGTTTCAGATATGGATAGTTCTTTGAAGATACGAGGTAGATTGTCACCATTTCCATTGAGTCCTTTGAGTCCATATGTGAGCAATCAAAGGACTAGAAGAGGAAGCTTGTCACCATTAGCATTGAGCCCTTTGAGTCCTTATGTAAGAACAACATATGGTCATGTCTCAGATTAA |
| Protein sequence |
>Msa0527760 MEQLRHIGEVLGSLKALMVLSDEIQINQRQCCLLHDIFTLAFDTIADEIRQNLKLEDRTTKWKPLECPLRELSRVFKEGEIYIKHCLDSKDWSGKALTLSQNRECVEFHIHNLLCYFPAVIEAIENAGEMSGLDQDENSKKKLMLARKYDVEWNDPKLFQWRFGKQYLVPKEICKQLENAWREDRWRLIEALKEKKGSSKLTSSKNDQHFADMLLKKLMNGNSDKTNHKNSNDKVLWPISVLLGSKDYQVRRRLGRGKEFKEIQWLGQSFALRHFLGEREAYENEISNLLSLSHPNILQYLCGFYDEEKKEFSLVTELMNKDLWTYMRENCGPRRQILFSIPVVVDLMLQMARGIEYLHSKNIYHGNLNPCNILLKARNSQEGYFQAKVVGFGLSSVENGEIYNASRSSPTHNPIGEEINPIIWYAPEVLTELEQMKNASTSCKYSEKADAYSFGMICFELLTGKIPFEDNHFQGIRTNQNIKAGERPLFPYRSPKYLVSLIKKCWQTDPSQRPGFSSICRILRYIKKFLSMNTEYVLINPELNQLELQNPPVDCCDIEGIFLKSFPVERTYNMSCVSQIPYEMFAYKVVEKGKINNQNLSNNTIPTIDNKGSESEPTKDVATVRSEESDDQSTVCEDVDASIIKDLVTYPKSISDDTQSIFYDTPSKKNVTIKKPSQMKPKKDQGTPKLQATKSLPPTLSGRVSRTNKANQSSLTSSSITPVKRRPPKVSESERKSNINKGNQSASVSTSSSVRKKVYGGNVSDSKPSLNLKTRDQSPFNRLKTKKSQLTMNSSSSPTRIRRVNSTNNSIVRMQKGFISTPPMSPSRSYTRRCCHVSDMDSSLKIRGRLSPFPLSPLSPYVSNQRTRRGSLSPLALSPLSPYVRTTYGHVSD* |
