AlfalfaGEDB | Msa0528150

M. sativa cultivar zhongmu-4 / Msa0528150

KEGG: K04733
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009856 GO:0009987 GO:0010183 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0030427 GO:0032501 GO:0032502 GO:0035838 GO:0036211 GO:0038023 GO:0040011 GO:0042221 GO:0042330 GO:0042995 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044463 GO:0044464 GO:0044706 GO:0048856 GO:0048868 GO:0050789 GO:0050794 GO:0050896 GO:0050918 GO:0051286 GO:0051704 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0090404 GO:0090406 GO:0120025 GO:0120038 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528150	XP_039683964.1	96.880	609	19	0	1	609	1	609	0.0	1216

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528150	sp\|Q9SFT7\|PBL26_ARATH	62.667	375	123	3	1	358	1	375	5.95e-165	479

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528150	G7L303	96.880	609	19	0	1	609	1	609	0.0	1216

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528150	MtrunA17_Chr7g0275131	96.880	609	19	0	1	609	1	609	0.0	1216
Msa0528150	MtrunA17_Chr1g0189791	52.742	620	259	11	1	603	1	603	0.0	562
Msa0528150	MtrunA17_Chr7g0237401	59.000	400	140	3	1	376	1	400	1.94e-159	463
Msa0528150	MtrunA17_Chr2g0286281	60.548	365	129	2	3	352	4	368	4.13e-150	442
Msa0528150	MtrunA17_Chr1g0213991	56.658	383	126	4	1	351	1	375	3.17e-143	424
Msa0528150	MtrunA17_Chr7g0263521	58.496	359	133	4	1	351	1	351	4.41e-141	414
Msa0528150	MtrunA17_Chr1g0181821	62.791	301	112	0	52	352	53	353	2.20e-137	405
Msa0528150	MtrunA17_Chr2g0327471	60.339	295	117	0	58	352	76	370	3.02e-129	385
Msa0528150	MtrunA17_Chr3g0143961	52.419	372	157	3	1	358	1	366	4.28e-129	384
Msa0528150	MtrunA17_Chr5g0443691	52.542	354	162	3	1	352	30	379	2.78e-127	380

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528150	AT3G07070.1	62.667	375	123	3	1	358	1	375	6.04e-166	479
Msa0528150	AT3G02810.1	62.903	372	114	4	1	364	1	356	3.15e-161	473
Msa0528150	AT5G16500.1	60.705	369	132	4	1	364	5	365	2.08e-155	461
Msa0528150	AT3G24790.2	63.714	350	119	2	1	350	1	342	1.40e-151	442
Msa0528150	AT3G24790.1	70.134	298	89	0	53	350	27	324	2.18e-148	433
Msa0528150	AT4G13190.1	60.331	363	120	3	1	352	1	350	4.27e-148	433
Msa0528150	AT5G18610.1	59.669	362	133	3	2	352	3	362	2.20e-147	436
Msa0528150	AT5G18610.2	59.669	362	133	3	2	352	3	362	2.20e-147	436
Msa0528150	AT5G18610.3	59.669	362	133	3	2	352	3	362	2.20e-147	436
Msa0528150	AT5G02800.1	62.928	321	112	2	55	368	55	375	5.77e-141	414

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Find 120 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGACCACAAAAGCAGT+AGG	0.603440	4_1:+865266	Msa0528150:CDS
AAAGGAACAATTCCTGCTAC+AGG	0.513896	4_1:+862915	Msa0528150:CDS
AAATCAGATGTGTACAGTTT+TGG	0.283384	4_1:+864236	Msa0528150:CDS
AAATCCTTCAATTATATACA+GGG	0.655416	4_1:+864043	Msa0528150:CDS
AACAATTCCTGCTACAGGAC+AGG	0.494209	4_1:+862920	Msa0528150:CDS
AACCTTATAACCAGAACACC+AGG	0.626594	4_1:+862584	Msa0528150:CDS
AACGGTCCCCTTCCTTGATT+TGG	0.276605	4_1:-865206	None:intergenic
AAGCAGCACCAAATCAAGGA+AGG	0.552995	4_1:+865198	Msa0528150:CDS
AAGTAAAGAATTTCTGGTTG+AGG	0.511705	4_1:+863103	Msa0528150:CDS
AAGTCCCTGTATATAATTGA+AGG	0.426982	4_1:-864047	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTAATTACCTGGTGTTC+TGG	-	chr4_1:862597-862616	None:intergenic	30.0%
GAATATTTGATGTGTCAACT+TGG	-	chr4_1:862800-862819	None:intergenic	30.0%
TTTCGTCAAGAATGTTTGAT+GGG	+	chr4_1:862867-862886	Msa0528150:CDS	30.0%
TCAAGGAAGTAAAGAATTTC+TGG	+	chr4_1:863097-863116	Msa0528150:CDS	30.0%
TGAGTTTGACAAGATTATCA+TGG	-	chr4_1:863151-863170	None:intergenic	30.0%
ATGATCAAGATCCATTAGAT+TGG	+	chr4_1:863958-863977	Msa0528150:CDS	30.0%
ATTCTGCTATACCAATCTAA+TGG	-	chr4_1:863972-863991	None:intergenic	30.0%
AAGTCCCTGTATATAATTGA+AGG	-	chr4_1:864050-864069	None:intergenic	30.0%
ATATTGGAGAAATTGCACTT+AGG	-	chr4_1:864618-864637	None:intergenic	30.0%
TCCTAAAAGATATGGAGATA+TGG	+	chr4_1:864739-864758	Msa0528150:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	862494	865675	862494	ID=Msa0528150;Name=Msa0528150
chr4_1	mRNA	862494	865675	862494	ID=Msa0528150-mRNA-1;Parent=Msa0528150;Name=Msa0528150-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|0\|0\|1\|1\|1\|5\|0\|609
chr4_1	exon	862494	862605	862494	ID=Msa0528150-mRNA-1:exon:199;Parent=Msa0528150-mRNA-1
chr4_1	exon	862763	862941	862763	ID=Msa0528150-mRNA-1:exon:200;Parent=Msa0528150-mRNA-1
chr4_1	exon	863062	863251	863062	ID=Msa0528150-mRNA-1:exon:201;Parent=Msa0528150-mRNA-1
chr4_1	exon	863949	864340	863949	ID=Msa0528150-mRNA-1:exon:202;Parent=Msa0528150-mRNA-1
chr4_1	exon	864719	865675	864719	ID=Msa0528150-mRNA-1:exon:203;Parent=Msa0528150-mRNA-1
chr4_1	CDS	862494	862605	862494	ID=Msa0528150-mRNA-1:cds;Parent=Msa0528150-mRNA-1
chr4_1	CDS	862763	862941	862763	ID=Msa0528150-mRNA-1:cds;Parent=Msa0528150-mRNA-1
chr4_1	CDS	863062	863251	863062	ID=Msa0528150-mRNA-1:cds;Parent=Msa0528150-mRNA-1
chr4_1	CDS	863949	864340	863949	ID=Msa0528150-mRNA-1:cds;Parent=Msa0528150-mRNA-1
chr4_1	CDS	864719	865675	864719	ID=Msa0528150-mRNA-1:cds;Parent=Msa0528150-mRNA-1

Gene Sequence

>Msa0528150

ATGAATTGCTTTCCTTGTTTTACCTCTCAAAAGAGTAAGAAAAGCAATAGCAGGAGGGAAAATGGGGTTGCACCACCTCAAGAAAATAATAACCTTATAACCAGAACACCAGATATCAAGAAACCAAAGCCAGCTGATGATCCAAACCAAGTTGACACATCAAATATTCAAGCACAAAATTTCACATTTCGCGAGTTAGCAATCGCAACAAAGAATTTTCGTCAAGAATGTTTGATGGGTGAAGGTGGTTTTGGTAGAGTTTACAAAGGAACAATTCCTGCTACAGGACAGGTGGTAGCAGTGAAGCAACTTGATCGAAATGGGATTCAAGGAAGTAAAGAATTTCTGGTTGAGGTTTTGATGTTGAGTCTCTTAAACCATGATAATCTTGTCAAACTCACTGGTTATTGTGCTGATGGTGATCAACGTCTTTTGGTCTATGAATTCATGTCAGGGGGCTCTCTAGAAAGTTGTCTTCTTGAGCGAAGAAATGATCAAGATCCATTAGATTGGTATAGCAGAATGAAAATAGCATCTAGTACTGCAAAAGGATTGTGGTACTTGCACGACAAGGCAAATCCTTCAATTATATACAGGGACTTGAAATCGTCGAACATTTTGCTTGATAAAGATCTTAATGCAAAATTATCTGATTACGGACTTGCCAAACTTGCTGGTAAAGATAAGGCTAACATTGTGCCTACAAGAGTCATGGGTACTTATGGTTATAGTGCCCCTGAGTATGTAAGAACAGGTAACCTCACCTTGAAATCAGATGTGTACAGTTTTGGAGTTGTCTTGCTAGAGCTCATTACCGGAAGACGAGCCGTTGACACCACAAGATCGCACGACGAGCAAAATCTAGTTTCATGGGCACAACCAATATTTAGAGATCCTAAAAGATATGGAGATATGGCAGATCCAAATCTGAATAAAAATTATCCAGAAAAGGATCTGAATCAAGTTGTTGCAATTGCAGCCATGTGCTTACAAGAGGAATCAGCAGCGCGTCCTTTGATGAGTGATGTTGTAACTGCTTTGAGTTTTCTTTCCACTTCTCCTCCTCCAGAACCTGTTCCTGCACCTCTTCCACCTCCCAATTCTACCTCCCAAAAGAGTGTTGCCACTGCCGATGAAAGTGAGAGCGAAAATGATAACGACAGCCATAGTTCCGATGAAGAGCGGTCTGTTCACGAGGATAACAAGTCTATTACAGCAGCTAGTGCAAAACACCAAGAACATGATGATGTCTCTGATACTGAACGGGATTATTATGATAATGAAAACCAACATGATTATAGTTCACAAGATGCTAAAGAAACAAAGGAGTTCTACTCCAAGTCAAGCCGCAAAAGCAGCACCAAATCAAGGAAGGGGACCGTTTCTTCTGGCCGAAGGAACAACTCCAGTTCAGATTCAGAAAATGACCACAAAAGCAGTAGGAGAAAGCATGATGTGGATGGAAGTTTAACTCAGAAAAGCAGCAAAAAATCTGCTGCAAGAGATTTAAGCCAGAAGAGCAGTAAGAAATCTACGGTAACAAAGGATTTAAGCCAAAAGAACAGTAAAAAATATTCTCCAAGTGTTGTAAGTCGATCATCTCATAGCAGCAGCGACGAGGAGTCACATGATGGAGGTGTTCTACTCAAGCGCGGTGACAGTAGGCCATCACATGATGGAAATGGATATTCCTTTGGCCTAGTGAGCAGTGATAGCGAGGAAGGATCACATTTTGATCGCATGAGCACGGGGGGGTCTGAGGAGGGAAGCTTTCATCATTTCCAACATTCCAGCAGCAGAAAATCAGATGAGGGAAGTGTTCATTCATGA

Protein sequence

>Msa0528150

MNCFPCFTSQKSKKSNSRRENGVAPPQENNNLITRTPDIKKPKPADDPNQVDTSNIQAQNFTFRELAIATKNFRQECLMGEGGFGRVYKGTIPATGQVVAVKQLDRNGIQGSKEFLVEVLMLSLLNHDNLVKLTGYCADGDQRLLVYEFMSGGSLESCLLERRNDQDPLDWYSRMKIASSTAKGLWYLHDKANPSIIYRDLKSSNILLDKDLNAKLSDYGLAKLAGKDKANIVPTRVMGTYGYSAPEYVRTGNLTLKSDVYSFGVVLLELITGRRAVDTTRSHDEQNLVSWAQPIFRDPKRYGDMADPNLNKNYPEKDLNQVVAIAAMCLQEESAARPLMSDVVTALSFLSTSPPPEPVPAPLPPPNSTSQKSVATADESESENDNDSHSSDEERSVHEDNKSITAASAKHQEHDDVSDTERDYYDNENQHDYSSQDAKETKEFYSKSSRKSSTKSRKGTVSSGRRNNSSSDSENDHKSSRRKHDVDGSLTQKSSKKSAARDLSQKSSKKSTVTKDLSQKNSKKYSPSVVSRSSHSSSDEESHDGGVLLKRGDSRPSHDGNGYSFGLVSSDSEEGSHFDRMSTGGSEEGSFHHFQHSSSRKSDEGSVHS*