AlfalfaGEDB | Msa0528420

M. sativa cultivar zhongmu-4 / Msa0528420

KEGG: -
GO: GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0005911 GO:0009505 GO:0009506 GO:0016020 GO:0030054 GO:0030312 GO:0044464 GO:0055044 GO:0071944
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528420	MCH92448.1	94.737	190	10	0	1	190	13	202	1.58e-115	344
Msa0528420	MCH92448.1	96.364	110	4	0	257	366	94	203	3.56e-65	216

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528420	sp\|Q9SCT4\|IMK2_ARATH	75.145	173	43	0	1	173	638	810	3.87e-84	275
Msa0528420	sp\|Q9SCT4\|IMK2_ARATH	75.701	107	26	0	242	348	704	810	2.82e-47	174

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528420	A0A392N0G7	94.737	190	10	0	1	190	13	202	7.56e-116	344
Msa0528420	A0A392N0G7	96.364	110	4	0	257	366	94	203	1.70e-65	216

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528420	MtrunA17_Chr4g0005201	96.316	190	7	0	1	190	604	793	7.07e-115	352
Msa0528420	MtrunA17_Chr4g0005201	99.091	110	1	0	257	366	685	794	1.07e-66	224
Msa0528420	MtrunA17_Chr5g0437741	71.579	190	54	0	1	190	596	785	2.85e-87	279
Msa0528420	MtrunA17_Chr5g0437741	72.650	117	32	0	250	366	670	786	5.51e-49	176
Msa0528420	MtrunA17_Chr6g0478621	46.243	173	83	4	3	171	482	648	8.72e-40	149
Msa0528420	MtrunA17_Chr6g0478621	51.000	100	45	2	250	346	550	648	3.20e-23	101
Msa0528420	MtrunA17_Chr4g0062911	45.143	175	86	4	1	171	449	617	1.19e-39	149
Msa0528420	MtrunA17_Chr4g0062911	48.515	101	48	2	249	346	518	617	4.46e-22	97.8
Msa0528420	MtrunA17_Chr4g0005221	75.000	100	24	1	268	366	449	548	1.65e-39	147
Msa0528420	MtrunA17_Chr4g0005221	71.717	99	27	1	93	190	449	547	5.43e-37	140

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0528420	AT3G51740.1	75.145	173	43	0	1	173	638	810	3.93e-85	275
Msa0528420	AT3G51740.1	75.701	107	26	0	242	348	704	810	2.87e-48	174
Msa0528420	AT2G26730.1	43.005	193	97	4	1	190	452	634	2.17e-41	154
Msa0528420	AT2G26730.1	43.802	121	60	3	249	366	520	635	2.87e-23	101
Msa0528420	AT3G08680.1	39.326	178	99	3	1	173	443	616	7.95e-37	141
Msa0528420	AT3G08680.1	46.602	103	52	1	249	348	514	616	1.01e-24	105
Msa0528420	AT3G08680.2	39.326	178	99	3	1	173	443	616	7.95e-37	141
Msa0528420	AT3G08680.2	46.602	103	52	1	249	348	514	616	1.01e-24	105
Msa0528420	AT3G24660.1	43.333	180	95	4	2	174	476	655	1.01e-35	138
Msa0528420	AT3G24660.1	44.762	105	53	3	250	349	551	655	1.16e-16	82.0

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Find 85 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGACGTTTACAGCCT+CGG	0.557756	4_1:-1221865	Msa0528420:CDS
AAACATATACCTGCTGCTCG+AGG	0.597492	4_1:-1225337	Msa0528420:five_prime_UTR
AAACCGAGCAAAGGGGAACG+AGG	0.663161	4_1:+1222880	None:intergenic
AACACCGAGGCTGTAAACGT+CGG	0.736422	4_1:+1225042	None:intergenic
AACATATACCTGCTGCTCGA+GGG	0.582736	4_1:-1225336	Msa0528420:five_prime_UTR
AACCAAGAGAATATAGTACA+TGG	0.582559	4_1:-1225238	Msa0528420:CDS
AAGACCGACGTTTACAGCCT+CGG	0.583782	4_1:-1225046	Msa0528420:CDS
AAGTCTTGGATACAATGCAC+CGG	0.639160	4_1:-1225098	Msa0528420:CDS
ACAAGGATGAAAATAGCAAT+TGG	0.434911	4_1:-1225289	Msa0528420:exon
ACCAACATCATTGCTACAGC+AGG	0.542435	4_1:-1225121	Msa0528420:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAGGATGAAAATAGCAAT+TGG	-	chr4_1:1221626-1221645	Msa0528420:CDS	30.0%
GTACTATATTCTCTTGGTTA+TGG	+	chr4_1:1221676-1221695	None:intergenic	30.0%
AACCAAGAGAATATAGTACA+TGG	-	chr4_1:1221677-1221696	Msa0528420:CDS	30.0%
TTCCATGTACTATATTCTCT+TGG	+	chr4_1:1221682-1221701	None:intergenic	30.0%
ACTATTGATGCAACATATTG+AGG	+	chr4_1:1221959-1221978	None:intergenic	30.0%
CACACTAAATGTGAAAAAGA+GGG	-	chr4_1:1222681-1222700	Msa0528420:intron	30.0%
TTATGGGATGATGACATTTA+TGG	-	chr4_1:1222846-1222865	Msa0528420:CDS	30.0%
ACATTTATGGTAAAAGCCTT+TGG	-	chr4_1:1222859-1222878	Msa0528420:CDS	30.0%
CTCATATTAGCAAATGTTGA+AGG	+	chr4_1:1223046-1223065	None:intergenic	30.0%
GTGCAGGTTATAAATTATAG+AGG	-	chr4_1:1223211-1223230	Msa0528420:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	1221552	1225385	1221552	ID=Msa0528420;Name=Msa0528420
chr4_1	mRNA	1221552	1225385	1221552	ID=Msa0528420-mRNA-1;Parent=Msa0528420;Name=Msa0528420-mRNA-1;_AED=0.16;_eAED=0.23;_QI=80\|0\|0\|1\|0\|0.33\|3\|0\|366
chr4_1	exon	1224748	1225385	1224748	ID=Msa0528420-mRNA-1:exon:356;Parent=Msa0528420-mRNA-1
chr4_1	exon	1222798	1222944	1222798	ID=Msa0528420-mRNA-1:exon:355;Parent=Msa0528420-mRNA-1
chr4_1	exon	1221552	1221944	1221552	ID=Msa0528420-mRNA-1:exon:354;Parent=Msa0528420-mRNA-1
chr4_1	five_prime_UTR	1225306	1225385	1225306	ID=Msa0528420-mRNA-1:five_prime_utr;Parent=Msa0528420-mRNA-1
chr4_1	CDS	1224748	1225305	1224748	ID=Msa0528420-mRNA-1:cds;Parent=Msa0528420-mRNA-1
chr4_1	CDS	1222798	1222944	1222798	ID=Msa0528420-mRNA-1:cds;Parent=Msa0528420-mRNA-1
chr4_1	CDS	1221552	1221944	1221552	ID=Msa0528420-mRNA-1:cds;Parent=Msa0528420-mRNA-1

Gene Sequence

>Msa0528420

ATGAAAATAGCAATTGGAATCGTCAATGGTCTCTCTTGCCTCCATAACCAAGAGAATATAGTACATGGAAATCTCACCTCAAGTAACATACTATTGGATGAGCAAACAAACCCTCACATCACAGATTTTTGTCTTTCAAGACTAATGACAACTTCAGCCAACACCAACATCATTGCTACAGCAGGAAGTCTTGGATACAATGCACCGGAGCTTTCAAAGACAAAGAAACCTACCACAAAGACCGACGTTTACAGCCTCGGTGTTATTCTTTTGGAGCTTTTAACTGGTAAACCACCAGGAGAACCAACAAATGGAATGGACTTGCCTCAATATGTTGCATCAATAGTTAAAGAAGAATGGACAAATGAAGTGTTTGATTTGGAACTCATGAGGGATGGACCAACGATAGGAGATGAACTTCTTAATACATTGAAATTAGCTTTTCATTGTGTTGATCCATCACCTTCTGCTAGGCCTGAAGTTAAACAAGTTTTGCAGCAGTTAGAGGAGATTAAGCCAGAACTGGTTGAAGTTGAAGTTGATGATGATGGAGCAAAGACAACAACAGATTCTGTAGTCGTTGATCTATGTTCTTCTGAACCTCGTTCCCCTTTGCTCGGTTTCATTTTCGGATTTGTACTTGGTGGGAACTGGTTAATTGTTGACATGTTGAAAACATCACTATCTGTGATGTTGAGGGGCAAGTACATGGAAATCTCACCTCAAGCAACATACTATTGGATGACAAAGAAACCTACCACAAAAACAGACGTTTACAGCCTCGGTGTTATCCTTTTGGAGCTCTTAACTGGTAAACCACCAGGAGAACCAACAAATGGAATGGACTTGCCTCAATATGTTGCATCAATAGTTAAAGAAGAATGGACAAATGAAGTGTTTGATTTGGAACTCATGAGGGATGGACCAACCATAGGAGATGAACTTCTTAATACATTGAAATTAGCTTTGCATTGTGTTGATCCATCACCTTCTGCTAGGCCTGAAGTTAAACAAGTTTTGCAGCAATTAGAGGAGATTAAACCAGAATTGGTTGAAGTTGAAGTTGATGATGATGCAGCAAAGGTTCAAACAAATGAA

Protein sequence

>Msa0528420

MKIAIGIVNGLSCLHNQENIVHGNLTSSNILLDEQTNPHITDFCLSRLMTTSANTNIIATAGSLGYNAPELSKTKKPTTKTDVYSLGVILLELLTGKPPGEPTNGMDLPQYVASIVKEEWTNEVFDLELMRDGPTIGDELLNTLKLAFHCVDPSPSARPEVKQVLQQLEEIKPELVEVEVDDDGAKTTTDSVVVDLCSSEPRSPLLGFIFGFVLGGNWLIVDMLKTSLSVMLRGKYMEISPQATYYWMTKKPTTKTDVYSLGVILLELLTGKPPGEPTNGMDLPQYVASIVKEEWTNEVFDLELMRDGPTIGDELLNTLKLALHCVDPSPSARPEVKQVLQQLEEIKPELVEVEVDDDAAKVQTNE