AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0529410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0529410 MtrunA17_Chr7g0222441 57.303 178 63 3 21 196 204 370 1.11e-55 186
Msa0529410 MtrunA17_Chr8g0358431 85.870 92 13 0 105 196 56 147 1.25e-51 165
Msa0529410 MtrunA17_Chr8g0356771 86.667 90 12 0 107 196 72 161 4.09e-51 162
Msa0529410 MtrunA17_Chr8g0341321 53.030 198 75 6 4 196 173 357 1.14e-50 172
Msa0529410 MtrunA17_Chr7g0228611 53.285 137 55 1 60 196 173 300 1.08e-38 139
Msa0529410 MtrunA17_Chr6g0458971 51.449 138 58 1 59 196 175 303 1.79e-36 133
Msa0529410 MtrunA17_Chr7g0271531 45.062 162 70 4 35 196 247 389 7.04e-35 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0529410 AT5G13110.1 56.522 184 62 5 19 196 200 371 1.09e-53 181
Msa0529410 AT1G24280.1 58.382 173 58 4 26 196 214 374 6.44e-53 179
Msa0529410 AT5G35790.1 54.054 185 67 5 18 196 180 352 2.91e-50 171
Msa0529410 AT5G40760.2 51.408 142 60 1 60 201 173 305 4.33e-38 138
Msa0529410 AT5G40760.1 51.408 142 60 1 60 201 173 305 4.33e-38 138
Msa0529410 AT3G27300.1 51.408 142 60 1 60 201 174 306 4.37e-38 138
Msa0529410 AT3G27300.3 51.408 142 60 1 60 201 174 306 4.37e-38 138
Msa0529410 AT3G27300.2 51.408 142 60 1 60 201 174 306 4.37e-38 138
Msa0529410 AT3G27300.5 51.408 142 60 1 60 201 228 360 6.29e-38 138
Msa0529410 AT3G27300.4 51.408 142 60 1 60 201 245 377 7.45e-38 138
Msa0529410 AT1G09420.2 57.843 102 39 2 97 197 316 414 1.25e-31 121
Msa0529410 AT1G09420.1 61.290 93 32 2 105 196 324 413 1.36e-30 118

Find 54 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATTGATTACTTTGAATT+TGG 0.131166 4_1:+2944375 Msa0529410:CDS
GATTACTTTGAATTTGGATA+TGG 0.198668 4_1:+2944381 Msa0529410:CDS
TTGATATTCTCAGAAGATTT+TGG 0.217136 4_1:+2945729 Msa0529410:CDS
CTCTGGTGTGGTTCTTATAT+TGG 0.220904 4_1:-2946343 None:intergenic
TGTAGGTACTTTGACAATTA+TGG 0.237604 4_1:+2945852 Msa0529410:intron
AGCAATATGGTGAAAGAAAA+TGG 0.245087 4_1:+2945115 Msa0529410:CDS
TTGTTGTTTGATTGTGGTTT+TGG 0.275624 4_1:+2944964 Msa0529410:CDS
GGGCAGCCGCGGCAAATGTC+TGG 0.287615 4_1:-2946424 None:intergenic
TGAGGTTATGAGATGGTTAT+TGG 0.298650 4_1:+2944935 Msa0529410:intron
TCCTCTACATCCAAACTAAC+AGG 0.318158 4_1:-2945939 None:intergenic
ATGGACTGCTGTGCTGGAAT+TGG 0.352828 4_1:+2944318 Msa0529410:CDS
ATTACATCCTCGAGTTTAAT+CGG 0.364307 4_1:-2946314 None:intergenic
ATTCTGAAGCGTGTAGAAAA+AGG 0.373678 4_1:+2944831 Msa0529410:CDS
TCACACGAAGACAATGAATT+TGG 0.374716 4_1:-2944406 None:intergenic
CAAATAGCTGAGAAGTTAGA+AGG 0.389483 4_1:+2945141 Msa0529410:CDS
GTCTGGGTTAAGTTGTCCTT+TGG 0.394579 4_1:-2946407 None:intergenic
TATAGGATCGATCACTATCT+TGG 0.396364 4_1:+2945615 Msa0529410:intron
AATGGAAACCCCTGTTAGTT+TGG 0.405110 4_1:+2945929 Msa0529410:CDS
ATTACTTTGAATTTGGATAT+GGG 0.418300 4_1:+2944382 Msa0529410:CDS
CTGTTACTTGACAGGGATGT+TGG 0.431891 4_1:+2944789 Msa0529410:intron
AGAAATGAAAAGGTCTCATA+TGG 0.448571 4_1:+2945966 Msa0529410:CDS
AGCTGAGAAGTTAGAAGGAA+GGG 0.452156 4_1:+2945146 Msa0529410:CDS
TGGATATGCAACACCTCCTC+TGG 0.463674 4_1:-2946360 None:intergenic
GCAATATGGTGAAAGAAAAT+GGG 0.474578 4_1:+2945116 Msa0529410:CDS
ATGATTAAAGGAAACTGATA+AGG 0.479594 4_1:+2945085 Msa0529410:intron
AGGGGTTTCCATTGCAAAGA+GGG 0.484242 4_1:-2945919 None:intergenic
CCCTGTTAGTTTGGATGTAG+AGG 0.489596 4_1:+2945938 Msa0529410:CDS
ATAAGGTTGGTGAAGCAATA+TGG 0.490665 4_1:+2945102 Msa0529410:CDS
CTTCATAGATAATGCAAGAT+GGG 0.490793 4_1:+2946448 Msa0529410:three_prime_UTR
AGAGGACATTAGAAATGAAA+AGG 0.504615 4_1:+2945956 Msa0529410:CDS
TAGATAATGCAAGATGGGAT+GGG 0.518772 4_1:+2946453 Msa0529410:three_prime_UTR
GGCAGCCGCGGCAAATGTCT+GGG 0.520346 4_1:-2946423 None:intergenic
AATACTAGCCCTCTTTGCAA+TGG 0.520791 4_1:+2945911 Msa0529410:CDS
ATCTATGAAGTGGGCAGCCG+CGG 0.523740 4_1:-2946435 None:intergenic
CTATCTTGGAAAAGAGCTTG+TGG 0.529498 4_1:+2945629 Msa0529410:CDS
TTAAAGGAAACTGATAAGGT+TGG 0.535969 4_1:+2945089 Msa0529410:intron
TAGCTGAGAAGTTAGAAGGA+AGG 0.540309 4_1:+2945145 Msa0529410:CDS
ATCTTGCATTATCTATGAAG+TGG 0.550073 4_1:-2946445 None:intergenic
ATAGATAATGCAAGATGGGA+TGG 0.550173 4_1:+2946452 Msa0529410:three_prime_UTR
TTGGTACTGAAGGACGTGGC+AGG 0.552068 4_1:+2945748 Msa0529410:CDS
GAGAAGTTAGAAGGAAGGGT+TGG 0.557009 4_1:+2945150 Msa0529410:CDS
ATGCAACACCTCCTCTGGTG+TGG 0.571539 4_1:-2946355 None:intergenic
ACTTCATAGATAATGCAAGA+TGG 0.584523 4_1:+2946447 Msa0529410:three_prime_UTR
CCTCTACATCCAAACTAACA+GGG 0.593680 4_1:-2945938 None:intergenic
AGATAATGCAAGATGGGATG+GGG 0.599941 4_1:+2946454 Msa0529410:three_prime_UTR
TGATGACAAAACTGTACCAA+AGG 0.605343 4_1:+2946391 Msa0529410:three_prime_UTR
CAGGGGTTTCCATTGCAAAG+AGG 0.613005 4_1:-2945920 None:intergenic
TCTTGCATTATCTATGAAGT+GGG 0.621537 4_1:-2946444 None:intergenic
TATAAGAACCACACCAGAGG+AGG 0.636996 4_1:+2946347 Msa0529410:three_prime_UTR
TATGAGACCGATTAAACTCG+AGG 0.649881 4_1:+2946307 Msa0529410:exon
CTCTACATCCAAACTAACAG+GGG 0.664372 4_1:-2945937 None:intergenic
CAATATAAGAACCACACCAG+AGG 0.676256 4_1:+2946344 Msa0529410:three_prime_UTR
CTTAACCCAGACATTTGCCG+CGG 0.679791 4_1:+2946418 Msa0529410:three_prime_UTR
ATGTATTGCCACGACCACAA+AGG 0.680638 4_1:-2945693 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAAAAAAGTAACA+GGG - chr4_1:2944755-2944774 None:intergenic 10.0%
!! AAAATAAAAAAAAAAGTAAC+AGG - chr4_1:2944756-2944775 None:intergenic 10.0%
!! TAAAATGGAAAATATTATAT+AGG + chr4_1:2945598-2945617 Msa0529410:intron 10.0%
!! AAATAAAAAAAAAAGTAACA+GGG - chr4_1:2944755-2944774 None:intergenic 10.0%
!! AAAATAAAAAAAAAAGTAAC+AGG - chr4_1:2944756-2944775 None:intergenic 10.0%
!! TAAAATGGAAAATATTATAT+AGG + chr4_1:2945598-2945617 Msa0529410:intron 10.0%
!!! ATTTAAAACAGATGAATTAA+AGG - chr4_1:2946227-2946246 None:intergenic 15.0%
!!! ATTTAAAACAGATGAATTAA+AGG - chr4_1:2946227-2946246 None:intergenic 15.0%
!! GAAATTGATTACTTTGAATT+TGG + chr4_1:2944375-2944394 Msa0529410:CDS 20.0%
!! ATTACTTTGAATTTGGATAT+GGG + chr4_1:2944382-2944401 Msa0529410:CDS 20.0%
!!! ATCTAAGATTTGATATGTTA+TGG + chr4_1:2944671-2944690 Msa0529410:intron 20.0%
!!! AATGATTTTCTTGATTACTA+AGG + chr4_1:2944894-2944913 Msa0529410:intron 20.0%
!! TTATTTGGTATGATGATTAA+AGG + chr4_1:2945073-2945092 Msa0529410:intron 20.0%
!!! TCTATTTTCATAAGCTATAT+TGG + chr4_1:2945406-2945425 Msa0529410:intron 20.0%
!!! GTAAAGTGCTTTATTTAAAA+TGG + chr4_1:2945583-2945602 Msa0529410:intron 20.0%
!! CAAAAATTAGATTTGAGAAT+CGG - chr4_1:2945671-2945690 None:intergenic 20.0%
!!! CACTTTTTATGATCTTAAAA+TGG + chr4_1:2945797-2945816 Msa0529410:intron 20.0%
!!! CATATGGTTACTTTTTTTAT+AGG + chr4_1:2945982-2946001 Msa0529410:intron 20.0%
!!! TTTCTACTTATATTTAAGGT+CGG - chr4_1:2946038-2946057 None:intergenic 20.0%
!! AACCTTTCTACTTATATTTA+AGG - chr4_1:2946042-2946061 None:intergenic 20.0%
!! GAAATTGATTACTTTGAATT+TGG + chr4_1:2944375-2944394 Msa0529410:CDS 20.0%
!! ATTACTTTGAATTTGGATAT+GGG + chr4_1:2944382-2944401 Msa0529410:CDS 20.0%
!!! ATCTAAGATTTGATATGTTA+TGG + chr4_1:2944671-2944690 Msa0529410:intron 20.0%
!!! AATGATTTTCTTGATTACTA+AGG + chr4_1:2944894-2944913 Msa0529410:intron 20.0%
!! TTATTTGGTATGATGATTAA+AGG + chr4_1:2945073-2945092 Msa0529410:intron 20.0%
!!! TCTATTTTCATAAGCTATAT+TGG + chr4_1:2945406-2945425 Msa0529410:intron 20.0%
!!! GTAAAGTGCTTTATTTAAAA+TGG + chr4_1:2945583-2945602 Msa0529410:intron 20.0%
!! CAAAAATTAGATTTGAGAAT+CGG - chr4_1:2945671-2945690 None:intergenic 20.0%
!!! CACTTTTTATGATCTTAAAA+TGG + chr4_1:2945797-2945816 Msa0529410:intron 20.0%
!!! CATATGGTTACTTTTTTTAT+AGG + chr4_1:2945982-2946001 Msa0529410:intron 20.0%
!!! TTTCTACTTATATTTAAGGT+CGG - chr4_1:2946038-2946057 None:intergenic 20.0%
!! AACCTTTCTACTTATATTTA+AGG - chr4_1:2946042-2946061 None:intergenic 20.0%
! GATTACTTTGAATTTGGATA+TGG + chr4_1:2944381-2944400 Msa0529410:CDS 25.0%
! CTTCAATTTATATGTCTTTG+TGG + chr4_1:2944468-2944487 Msa0529410:intron 25.0%
! ATTGAGTGAATTATATGTTC+AGG + chr4_1:2944552-2944571 Msa0529410:intron 25.0%
!! AACCTTTTTATTCACTATGT+TGG - chr4_1:2944640-2944659 None:intergenic 25.0%
!! TCAAGAAAATCATTCTTTTG+AGG - chr4_1:2944888-2944907 None:intergenic 25.0%
!!! TTTGTTTTGTTGTTTGATTG+TGG + chr4_1:2944958-2944977 Msa0529410:CDS 25.0%
! ATGATTAAAGGAAACTGATA+AGG + chr4_1:2945085-2945104 Msa0529410:intron 25.0%
! ACTTATATGATAAGTGTGAA+TGG - chr4_1:2945319-2945338 None:intergenic 25.0%
!! ATCTAATTTTTGAACCTTTG+TGG + chr4_1:2945679-2945698 Msa0529410:CDS 25.0%
!! TTGATATTCTCAGAAGATTT+TGG + chr4_1:2945729-2945748 Msa0529410:CDS 25.0%
! GACCTTAAATATAAGTAGAA+AGG + chr4_1:2946037-2946056 Msa0529410:intron 25.0%
!! ACAAATACATGTACCTTTTT+CGG + chr4_1:2946074-2946093 Msa0529410:intron 25.0%
!!! ACCTTTTTCGGTTAAATATA+AGG + chr4_1:2946086-2946105 Msa0529410:intron 25.0%
! ACCTTATATTTAACCGAAAA+AGG - chr4_1:2946090-2946109 None:intergenic 25.0%
!! TTTTCGGTTAAATATAAGGT+CGG + chr4_1:2946090-2946109 Msa0529410:intron 25.0%
! ACTTTCTTCTCAAAATCATT+TGG + chr4_1:2946150-2946169 Msa0529410:intron 25.0%
! CTTTCTTCTCAAAATCATTT+GGG + chr4_1:2946151-2946170 Msa0529410:intron 25.0%
! GATTACTTTGAATTTGGATA+TGG + chr4_1:2944381-2944400 Msa0529410:CDS 25.0%
! CTTCAATTTATATGTCTTTG+TGG + chr4_1:2944468-2944487 Msa0529410:intron 25.0%
! ATTGAGTGAATTATATGTTC+AGG + chr4_1:2944552-2944571 Msa0529410:intron 25.0%
!! AACCTTTTTATTCACTATGT+TGG - chr4_1:2944640-2944659 None:intergenic 25.0%
!! TCAAGAAAATCATTCTTTTG+AGG - chr4_1:2944888-2944907 None:intergenic 25.0%
!!! TTTGTTTTGTTGTTTGATTG+TGG + chr4_1:2944958-2944977 Msa0529410:CDS 25.0%
! ATGATTAAAGGAAACTGATA+AGG + chr4_1:2945085-2945104 Msa0529410:intron 25.0%
! ACTTATATGATAAGTGTGAA+TGG - chr4_1:2945319-2945338 None:intergenic 25.0%
!! ATCTAATTTTTGAACCTTTG+TGG + chr4_1:2945679-2945698 Msa0529410:CDS 25.0%
!! TTGATATTCTCAGAAGATTT+TGG + chr4_1:2945729-2945748 Msa0529410:CDS 25.0%
! GACCTTAAATATAAGTAGAA+AGG + chr4_1:2946037-2946056 Msa0529410:intron 25.0%
!! ACAAATACATGTACCTTTTT+CGG + chr4_1:2946074-2946093 Msa0529410:intron 25.0%
!!! ACCTTTTTCGGTTAAATATA+AGG + chr4_1:2946086-2946105 Msa0529410:intron 25.0%
! ACCTTATATTTAACCGAAAA+AGG - chr4_1:2946090-2946109 None:intergenic 25.0%
!! TTTTCGGTTAAATATAAGGT+CGG + chr4_1:2946090-2946109 Msa0529410:intron 25.0%
! ACTTTCTTCTCAAAATCATT+TGG + chr4_1:2946150-2946169 Msa0529410:intron 25.0%
! CTTTCTTCTCAAAATCATTT+GGG + chr4_1:2946151-2946170 Msa0529410:intron 25.0%
!! TTTATTGCAGCAGTTGATAA+AGG + chr4_1:2944598-2944617 Msa0529410:intron 30.0%
TGCCAACATAGTGAATAAAA+AGG + chr4_1:2944635-2944654 Msa0529410:intron 30.0%
!! AATGTTTTCTGTTACTTGAC+AGG + chr4_1:2944781-2944800 Msa0529410:intron 30.0%
!! ATGTTTTCTGTTACTTGACA+GGG + chr4_1:2944782-2944801 Msa0529410:intron 30.0%
!!! TTGTTGTTTGATTGTGGTTT+TGG + chr4_1:2944964-2944983 Msa0529410:CDS 30.0%
TTAAAGGAAACTGATAAGGT+TGG + chr4_1:2945089-2945108 Msa0529410:intron 30.0%
AGCAATATGGTGAAAGAAAA+TGG + chr4_1:2945115-2945134 Msa0529410:CDS 30.0%
GCAATATGGTGAAAGAAAAT+GGG + chr4_1:2945116-2945135 Msa0529410:CDS 30.0%
AAACCTGAAAATTTGATCCT+CGG - chr4_1:2945252-2945271 None:intergenic 30.0%
!! AACAATCCTAGAAGTGTTTA+TGG + chr4_1:2945497-2945516 Msa0529410:intron 30.0%
TAATTAGACCATGTTTGGAT+TGG - chr4_1:2945544-2945563 None:intergenic 30.0%
ACAGATAATTAGACCATGTT+TGG - chr4_1:2945549-2945568 None:intergenic 30.0%
! AAAAAGTGTAAATGCTGCAA+AGG - chr4_1:2945785-2945804 None:intergenic 30.0%
TGTAGGTACTTTGACAATTA+TGG + chr4_1:2945852-2945871 Msa0529410:intron 30.0%
AGAGGACATTAGAAATGAAA+AGG + chr4_1:2945956-2945975 Msa0529410:CDS 30.0%
AGAAATGAAAAGGTCTCATA+TGG + chr4_1:2945966-2945985 Msa0529410:CDS 30.0%
! TACTTATATTTAAGGTCGGA+GGG - chr4_1:2946034-2946053 None:intergenic 30.0%
CATGACTTTCAATTGCTTAA+TGG + chr4_1:2946260-2946279 Msa0529410:intron 30.0%
ATTACATCCTCGAGTTTAAT+CGG - chr4_1:2946317-2946336 None:intergenic 30.0%
! AAACTCGAGGATGTAATTTT+AGG + chr4_1:2946320-2946339 Msa0529410:exon 30.0%
!! TACAGTTTTGTCATCAATGT+AGG - chr4_1:2946387-2946406 None:intergenic 30.0%
TCTTGCATTATCTATGAAGT+GGG - chr4_1:2946447-2946466 None:intergenic 30.0%
ATCTTGCATTATCTATGAAG+TGG - chr4_1:2946448-2946467 None:intergenic 30.0%
ACTTCATAGATAATGCAAGA+TGG + chr4_1:2946447-2946466 Msa0529410:three_prime_UTR 30.0%
CTTCATAGATAATGCAAGAT+GGG + chr4_1:2946448-2946467 Msa0529410:three_prime_UTR 30.0%
!! TTTATTGCAGCAGTTGATAA+AGG + chr4_1:2944598-2944617 Msa0529410:intron 30.0%
TGCCAACATAGTGAATAAAA+AGG + chr4_1:2944635-2944654 Msa0529410:intron 30.0%
!! AATGTTTTCTGTTACTTGAC+AGG + chr4_1:2944781-2944800 Msa0529410:intron 30.0%
!! ATGTTTTCTGTTACTTGACA+GGG + chr4_1:2944782-2944801 Msa0529410:intron 30.0%
!!! TTGTTGTTTGATTGTGGTTT+TGG + chr4_1:2944964-2944983 Msa0529410:CDS 30.0%
TTAAAGGAAACTGATAAGGT+TGG + chr4_1:2945089-2945108 Msa0529410:intron 30.0%
AGCAATATGGTGAAAGAAAA+TGG + chr4_1:2945115-2945134 Msa0529410:CDS 30.0%
GCAATATGGTGAAAGAAAAT+GGG + chr4_1:2945116-2945135 Msa0529410:CDS 30.0%
AAACCTGAAAATTTGATCCT+CGG - chr4_1:2945252-2945271 None:intergenic 30.0%
!! AACAATCCTAGAAGTGTTTA+TGG + chr4_1:2945497-2945516 Msa0529410:intron 30.0%
TAATTAGACCATGTTTGGAT+TGG - chr4_1:2945544-2945563 None:intergenic 30.0%
ACAGATAATTAGACCATGTT+TGG - chr4_1:2945549-2945568 None:intergenic 30.0%
! AAAAAGTGTAAATGCTGCAA+AGG - chr4_1:2945785-2945804 None:intergenic 30.0%
TGTAGGTACTTTGACAATTA+TGG + chr4_1:2945852-2945871 Msa0529410:intron 30.0%
AGAGGACATTAGAAATGAAA+AGG + chr4_1:2945956-2945975 Msa0529410:CDS 30.0%
AGAAATGAAAAGGTCTCATA+TGG + chr4_1:2945966-2945985 Msa0529410:CDS 30.0%
! TACTTATATTTAAGGTCGGA+GGG - chr4_1:2946034-2946053 None:intergenic 30.0%
CATGACTTTCAATTGCTTAA+TGG + chr4_1:2946260-2946279 Msa0529410:intron 30.0%
ATTACATCCTCGAGTTTAAT+CGG - chr4_1:2946317-2946336 None:intergenic 30.0%
! AAACTCGAGGATGTAATTTT+AGG + chr4_1:2946320-2946339 Msa0529410:exon 30.0%
!! TACAGTTTTGTCATCAATGT+AGG - chr4_1:2946387-2946406 None:intergenic 30.0%
TCTTGCATTATCTATGAAGT+GGG - chr4_1:2946447-2946466 None:intergenic 30.0%
ATCTTGCATTATCTATGAAG+TGG - chr4_1:2946448-2946467 None:intergenic 30.0%
ACTTCATAGATAATGCAAGA+TGG + chr4_1:2946447-2946466 Msa0529410:three_prime_UTR 30.0%
CTTCATAGATAATGCAAGAT+GGG + chr4_1:2946448-2946467 Msa0529410:three_prime_UTR 30.0%
TCACACGAAGACAATGAATT+TGG - chr4_1:2944409-2944428 None:intergenic 35.0%
ATAAAAGAAGCTTGCAGTTC+TGG - chr4_1:2944583-2944602 None:intergenic 35.0%
GTAACAGGGAACATATCATT+AGG - chr4_1:2944741-2944760 None:intergenic 35.0%
!! ATTCTGAAGCGTGTAGAAAA+AGG + chr4_1:2944831-2944850 Msa0529410:CDS 35.0%
ACTAAGGTAGCAGTTTATGT+TGG + chr4_1:2944910-2944929 Msa0529410:intron 35.0%
!! TAGCAGTTTATGTTGGTTTG+AGG + chr4_1:2944917-2944936 Msa0529410:intron 35.0%
TGAGGTTATGAGATGGTTAT+TGG + chr4_1:2944935-2944954 Msa0529410:intron 35.0%
!! ATAAGGTTGGTGAAGCAATA+TGG + chr4_1:2945102-2945121 Msa0529410:CDS 35.0%
CAAATAGCTGAGAAGTTAGA+AGG + chr4_1:2945141-2945160 Msa0529410:CDS 35.0%
AACTACACTTTGACTGTGTT+TGG + chr4_1:2945275-2945294 Msa0529410:intron 35.0%
!! ACACTTCTAGGATTGTTTGA+AGG - chr4_1:2945494-2945513 None:intergenic 35.0%
CAAACAATCCAATCCAAACA+TGG + chr4_1:2945533-2945552 Msa0529410:intron 35.0%
TATAGGATCGATCACTATCT+TGG + chr4_1:2945615-2945634 Msa0529410:intron 35.0%
!! TCAGAAGATTTTGGTACTGA+AGG + chr4_1:2945738-2945757 Msa0529410:CDS 35.0%
TTCATATCTGTTCCATGTGT+AGG + chr4_1:2945835-2945854 Msa0529410:intron 35.0%
! ATTGTCAAAGTACCTACACA+TGG - chr4_1:2945850-2945869 None:intergenic 35.0%
! CTACTTATATTTAAGGTCGG+AGG - chr4_1:2946035-2946054 None:intergenic 35.0%
!!! AAAGTCCAGGTTTTAAACTC+TGG - chr4_1:2946135-2946154 None:intergenic 35.0%
!! TGATTTTGAGAAGAAAGTCC+AGG - chr4_1:2946148-2946167 None:intergenic 35.0%
TCTTCTCAAAATCATTTGGG+TGG + chr4_1:2946154-2946173 Msa0529410:intron 35.0%
! TCAATTGCTTAATGGATGTC+AGG + chr4_1:2946268-2946287 Msa0529410:intron 35.0%
TGATGACAAAACTGTACCAA+AGG + chr4_1:2946391-2946410 Msa0529410:three_prime_UTR 35.0%
ATAGATAATGCAAGATGGGA+TGG + chr4_1:2946452-2946471 Msa0529410:three_prime_UTR 35.0%
TAGATAATGCAAGATGGGAT+GGG + chr4_1:2946453-2946472 Msa0529410:three_prime_UTR 35.0%
!!! TACCTTTTTTGATGAAGGCT+GGG + chr4_1:2946477-2946496 Msa0529410:three_prime_UTR 35.0%
TCACACGAAGACAATGAATT+TGG - chr4_1:2944409-2944428 None:intergenic 35.0%
ATAAAAGAAGCTTGCAGTTC+TGG - chr4_1:2944583-2944602 None:intergenic 35.0%
GTAACAGGGAACATATCATT+AGG - chr4_1:2944741-2944760 None:intergenic 35.0%
!! ATTCTGAAGCGTGTAGAAAA+AGG + chr4_1:2944831-2944850 Msa0529410:CDS 35.0%
ACTAAGGTAGCAGTTTATGT+TGG + chr4_1:2944910-2944929 Msa0529410:intron 35.0%
!! TAGCAGTTTATGTTGGTTTG+AGG + chr4_1:2944917-2944936 Msa0529410:intron 35.0%
TGAGGTTATGAGATGGTTAT+TGG + chr4_1:2944935-2944954 Msa0529410:intron 35.0%
!! ATAAGGTTGGTGAAGCAATA+TGG + chr4_1:2945102-2945121 Msa0529410:CDS 35.0%
CAAATAGCTGAGAAGTTAGA+AGG + chr4_1:2945141-2945160 Msa0529410:CDS 35.0%
AACTACACTTTGACTGTGTT+TGG + chr4_1:2945275-2945294 Msa0529410:intron 35.0%
!! ACACTTCTAGGATTGTTTGA+AGG - chr4_1:2945494-2945513 None:intergenic 35.0%
CAAACAATCCAATCCAAACA+TGG + chr4_1:2945533-2945552 Msa0529410:intron 35.0%
TATAGGATCGATCACTATCT+TGG + chr4_1:2945615-2945634 Msa0529410:intron 35.0%
!! TCAGAAGATTTTGGTACTGA+AGG + chr4_1:2945738-2945757 Msa0529410:CDS 35.0%
TTCATATCTGTTCCATGTGT+AGG + chr4_1:2945835-2945854 Msa0529410:intron 35.0%
! ATTGTCAAAGTACCTACACA+TGG - chr4_1:2945850-2945869 None:intergenic 35.0%
! CTACTTATATTTAAGGTCGG+AGG - chr4_1:2946035-2946054 None:intergenic 35.0%
!!! AAAGTCCAGGTTTTAAACTC+TGG - chr4_1:2946135-2946154 None:intergenic 35.0%
!! TGATTTTGAGAAGAAAGTCC+AGG - chr4_1:2946148-2946167 None:intergenic 35.0%
TCTTCTCAAAATCATTTGGG+TGG + chr4_1:2946154-2946173 Msa0529410:intron 35.0%
! TCAATTGCTTAATGGATGTC+AGG + chr4_1:2946268-2946287 Msa0529410:intron 35.0%
TGATGACAAAACTGTACCAA+AGG + chr4_1:2946391-2946410 Msa0529410:three_prime_UTR 35.0%
ATAGATAATGCAAGATGGGA+TGG + chr4_1:2946452-2946471 Msa0529410:three_prime_UTR 35.0%
TAGATAATGCAAGATGGGAT+GGG + chr4_1:2946453-2946472 Msa0529410:three_prime_UTR 35.0%
!!! TACCTTTTTTGATGAAGGCT+GGG + chr4_1:2946477-2946496 Msa0529410:three_prime_UTR 35.0%
CATTGTCTTCGTGTGAGTAT+TGG + chr4_1:2944413-2944432 Msa0529410:intron 40.0%
TGAAGCGTGTAGAAAAAGGT+TGG + chr4_1:2944835-2944854 Msa0529410:CDS 40.0%
GAAGCGTGTAGAAAAAGGTT+GGG + chr4_1:2944836-2944855 Msa0529410:CDS 40.0%
AAGCGTGTAGAAAAAGGTTG+GGG + chr4_1:2944837-2944856 Msa0529410:CDS 40.0%
!! GTTGGTTTGAGGTTATGAGA+TGG + chr4_1:2944928-2944947 Msa0529410:intron 40.0%
TAGCTGAGAAGTTAGAAGGA+AGG + chr4_1:2945145-2945164 Msa0529410:CDS 40.0%
AGCTGAGAAGTTAGAAGGAA+GGG + chr4_1:2945146-2945165 Msa0529410:CDS 40.0%
TGATCTTGTTAAAGCAGCTG+AGG - chr4_1:2945213-2945232 None:intergenic 40.0%
! TGACCGAGGATCAAATTTTC+AGG + chr4_1:2945246-2945265 Msa0529410:intron 40.0%
CTATCTTGGAAAAGAGCTTG+TGG + chr4_1:2945629-2945648 Msa0529410:CDS 40.0%
! TTTTTGAACCTTTGTGGTCG+TGG + chr4_1:2945685-2945704 Msa0529410:CDS 40.0%
AATACTAGCCCTCTTTGCAA+TGG + chr4_1:2945911-2945930 Msa0529410:CDS 40.0%
AATGGAAACCCCTGTTAGTT+TGG + chr4_1:2945929-2945948 Msa0529410:CDS 40.0%
CTCTACATCCAAACTAACAG+GGG - chr4_1:2945940-2945959 None:intergenic 40.0%
CCTCTACATCCAAACTAACA+GGG - chr4_1:2945941-2945960 None:intergenic 40.0%
TCCTCTACATCCAAACTAAC+AGG - chr4_1:2945942-2945961 None:intergenic 40.0%
TCGGTTAAATATAAGGTCGG+AGG + chr4_1:2946093-2946112 Msa0529410:intron 40.0%
! AGAAGCCAGAGTTTAAAACC+TGG + chr4_1:2946127-2946146 Msa0529410:intron 40.0%
TATGAGACCGATTAAACTCG+AGG + chr4_1:2946307-2946326 Msa0529410:exon 40.0%
CTCTGGTGTGGTTCTTATAT+TGG - chr4_1:2946346-2946365 None:intergenic 40.0%
CAATATAAGAACCACACCAG+AGG + chr4_1:2946344-2946363 Msa0529410:three_prime_UTR 40.0%
! GTTTTGTCATCAATGTAGGC+TGG - chr4_1:2946383-2946402 None:intergenic 40.0%
AGATAATGCAAGATGGGATG+GGG + chr4_1:2946454-2946473 Msa0529410:three_prime_UTR 40.0%
!!! TGGGGTACCTTTTTTGATGA+AGG + chr4_1:2946472-2946491 Msa0529410:three_prime_UTR 40.0%
!!! GTACCTTTTTTGATGAAGGC+TGG + chr4_1:2946476-2946495 Msa0529410:three_prime_UTR 40.0%
TTCCCAGCCTTCATCAAAAA+AGG - chr4_1:2946482-2946501 None:intergenic 40.0%
CATTGTCTTCGTGTGAGTAT+TGG + chr4_1:2944413-2944432 Msa0529410:intron 40.0%
TGAAGCGTGTAGAAAAAGGT+TGG + chr4_1:2944835-2944854 Msa0529410:CDS 40.0%
GAAGCGTGTAGAAAAAGGTT+GGG + chr4_1:2944836-2944855 Msa0529410:CDS 40.0%
AAGCGTGTAGAAAAAGGTTG+GGG + chr4_1:2944837-2944856 Msa0529410:CDS 40.0%
!! GTTGGTTTGAGGTTATGAGA+TGG + chr4_1:2944928-2944947 Msa0529410:intron 40.0%
TAGCTGAGAAGTTAGAAGGA+AGG + chr4_1:2945145-2945164 Msa0529410:CDS 40.0%
AGCTGAGAAGTTAGAAGGAA+GGG + chr4_1:2945146-2945165 Msa0529410:CDS 40.0%
TGATCTTGTTAAAGCAGCTG+AGG - chr4_1:2945213-2945232 None:intergenic 40.0%
! TGACCGAGGATCAAATTTTC+AGG + chr4_1:2945246-2945265 Msa0529410:intron 40.0%
CTATCTTGGAAAAGAGCTTG+TGG + chr4_1:2945629-2945648 Msa0529410:CDS 40.0%
! TTTTTGAACCTTTGTGGTCG+TGG + chr4_1:2945685-2945704 Msa0529410:CDS 40.0%
AATACTAGCCCTCTTTGCAA+TGG + chr4_1:2945911-2945930 Msa0529410:CDS 40.0%
AATGGAAACCCCTGTTAGTT+TGG + chr4_1:2945929-2945948 Msa0529410:CDS 40.0%
CTCTACATCCAAACTAACAG+GGG - chr4_1:2945940-2945959 None:intergenic 40.0%
CCTCTACATCCAAACTAACA+GGG - chr4_1:2945941-2945960 None:intergenic 40.0%
TCCTCTACATCCAAACTAAC+AGG - chr4_1:2945942-2945961 None:intergenic 40.0%
TCGGTTAAATATAAGGTCGG+AGG + chr4_1:2946093-2946112 Msa0529410:intron 40.0%
! AGAAGCCAGAGTTTAAAACC+TGG + chr4_1:2946127-2946146 Msa0529410:intron 40.0%
TATGAGACCGATTAAACTCG+AGG + chr4_1:2946307-2946326 Msa0529410:exon 40.0%
CTCTGGTGTGGTTCTTATAT+TGG - chr4_1:2946346-2946365 None:intergenic 40.0%
CAATATAAGAACCACACCAG+AGG + chr4_1:2946344-2946363 Msa0529410:three_prime_UTR 40.0%
! GTTTTGTCATCAATGTAGGC+TGG - chr4_1:2946383-2946402 None:intergenic 40.0%
AGATAATGCAAGATGGGATG+GGG + chr4_1:2946454-2946473 Msa0529410:three_prime_UTR 40.0%
!!! TGGGGTACCTTTTTTGATGA+AGG + chr4_1:2946472-2946491 Msa0529410:three_prime_UTR 40.0%
!!! GTACCTTTTTTGATGAAGGC+TGG + chr4_1:2946476-2946495 Msa0529410:three_prime_UTR 40.0%
TTCCCAGCCTTCATCAAAAA+AGG - chr4_1:2946482-2946501 None:intergenic 40.0%
! AAAGAAGCTTGCAGTTCTGG+AGG - chr4_1:2944580-2944599 None:intergenic 45.0%
! CTGTTACTTGACAGGGATGT+TGG + chr4_1:2944789-2944808 Msa0529410:intron 45.0%
GTAGAAAAAGGTTGGGGTCA+AGG + chr4_1:2944843-2944862 Msa0529410:CDS 45.0%
GAGAAGTTAGAAGGAAGGGT+TGG + chr4_1:2945150-2945169 Msa0529410:CDS 45.0%
ATTCAAGCAGTATCTGACCG+AGG + chr4_1:2945232-2945251 Msa0529410:intron 45.0%
GGATTGTTTGAAGGAGCTCA+TGG - chr4_1:2945485-2945504 None:intergenic 45.0%
CCACTGCCATAAACACTTCT+AGG - chr4_1:2945506-2945525 None:intergenic 45.0%
!! CCTAGAAGTGTTTATGGCAG+TGG + chr4_1:2945503-2945522 Msa0529410:intron 45.0%
ATGTATTGCCACGACCACAA+AGG - chr4_1:2945696-2945715 None:intergenic 45.0%
!! GATTTTGGTACTGAAGGACG+TGG + chr4_1:2945744-2945763 Msa0529410:CDS 45.0%
AGGGGTTTCCATTGCAAAGA+GGG - chr4_1:2945922-2945941 None:intergenic 45.0%
! CCCTGTTAGTTTGGATGTAG+AGG + chr4_1:2945938-2945957 Msa0529410:CDS 45.0%
TCCATAACCATGAGTCTCTG+AGG - chr4_1:2946204-2946223 None:intergenic 45.0%
! GCTTAATGGATGTCAGGTCA+AGG + chr4_1:2946274-2946293 Msa0529410:intron 45.0%
TATAAGAACCACACCAGAGG+AGG + chr4_1:2946347-2946366 Msa0529410:three_prime_UTR 45.0%
! GTCTGGGTTAAGTTGTCCTT+TGG - chr4_1:2946410-2946429 None:intergenic 45.0%
! AAAGAAGCTTGCAGTTCTGG+AGG - chr4_1:2944580-2944599 None:intergenic 45.0%
! CTGTTACTTGACAGGGATGT+TGG + chr4_1:2944789-2944808 Msa0529410:intron 45.0%
GTAGAAAAAGGTTGGGGTCA+AGG + chr4_1:2944843-2944862 Msa0529410:CDS 45.0%
GAGAAGTTAGAAGGAAGGGT+TGG + chr4_1:2945150-2945169 Msa0529410:CDS 45.0%
ATTCAAGCAGTATCTGACCG+AGG + chr4_1:2945232-2945251 Msa0529410:intron 45.0%
GGATTGTTTGAAGGAGCTCA+TGG - chr4_1:2945485-2945504 None:intergenic 45.0%
CCACTGCCATAAACACTTCT+AGG - chr4_1:2945506-2945525 None:intergenic 45.0%
!! CCTAGAAGTGTTTATGGCAG+TGG + chr4_1:2945503-2945522 Msa0529410:intron 45.0%
ATGTATTGCCACGACCACAA+AGG - chr4_1:2945696-2945715 None:intergenic 45.0%
!! GATTTTGGTACTGAAGGACG+TGG + chr4_1:2945744-2945763 Msa0529410:CDS 45.0%
AGGGGTTTCCATTGCAAAGA+GGG - chr4_1:2945922-2945941 None:intergenic 45.0%
! CCCTGTTAGTTTGGATGTAG+AGG + chr4_1:2945938-2945957 Msa0529410:CDS 45.0%
TCCATAACCATGAGTCTCTG+AGG - chr4_1:2946204-2946223 None:intergenic 45.0%
! GCTTAATGGATGTCAGGTCA+AGG + chr4_1:2946274-2946293 Msa0529410:intron 45.0%
TATAAGAACCACACCAGAGG+AGG + chr4_1:2946347-2946366 Msa0529410:three_prime_UTR 45.0%
! GTCTGGGTTAAGTTGTCCTT+TGG - chr4_1:2946410-2946429 None:intergenic 45.0%
!!! TTTTATTAATATGTTTTATT+TGG + chr4_1:2945058-2945077 Msa0529410:intron 5.0%
!!! TTTTATTAATATGTTTTATT+TGG + chr4_1:2945058-2945077 Msa0529410:intron 5.0%
! ATGGACTGCTGTGCTGGAAT+TGG + chr4_1:2944318-2944337 Msa0529410:CDS 50.0%
CAGGGGTTTCCATTGCAAAG+AGG - chr4_1:2945923-2945942 None:intergenic 50.0%
GCCTCAGAGACTCATGGTTA+TGG + chr4_1:2946200-2946219 Msa0529410:intron 50.0%
TGGATATGCAACACCTCCTC+TGG - chr4_1:2946363-2946382 None:intergenic 50.0%
CTTAACCCAGACATTTGCCG+CGG + chr4_1:2946418-2946437 Msa0529410:three_prime_UTR 50.0%
CCAGCACAGCAGTCCATAAA+TGG - chr4_1:2944315-2944334 None:intergenic 50.0%
! ATGGACTGCTGTGCTGGAAT+TGG + chr4_1:2944318-2944337 Msa0529410:CDS 50.0%
CAGGGGTTTCCATTGCAAAG+AGG - chr4_1:2945923-2945942 None:intergenic 50.0%
GCCTCAGAGACTCATGGTTA+TGG + chr4_1:2946200-2946219 Msa0529410:intron 50.0%
TGGATATGCAACACCTCCTC+TGG - chr4_1:2946363-2946382 None:intergenic 50.0%
CTTAACCCAGACATTTGCCG+CGG + chr4_1:2946418-2946437 Msa0529410:three_prime_UTR 50.0%
GCAAGCAGTGTAGAGAGCGA+TGG + chr4_1:2945172-2945191 Msa0529410:intron 55.0%
! TTGGTACTGAAGGACGTGGC+AGG + chr4_1:2945748-2945767 Msa0529410:CDS 55.0%
AGCTACGCCTCAGAGACTCA+TGG + chr4_1:2946194-2946213 Msa0529410:intron 55.0%
ATGCAACACCTCCTCTGGTG+TGG - chr4_1:2946358-2946377 None:intergenic 55.0%
ATCTATGAAGTGGGCAGCCG+CGG - chr4_1:2946438-2946457 None:intergenic 55.0%
GCAAGCAGTGTAGAGAGCGA+TGG + chr4_1:2945172-2945191 Msa0529410:intron 55.0%
! TTGGTACTGAAGGACGTGGC+AGG + chr4_1:2945748-2945767 Msa0529410:CDS 55.0%
AGCTACGCCTCAGAGACTCA+TGG + chr4_1:2946194-2946213 Msa0529410:intron 55.0%
ATGCAACACCTCCTCTGGTG+TGG - chr4_1:2946358-2946377 None:intergenic 55.0%
ATCTATGAAGTGGGCAGCCG+CGG - chr4_1:2946438-2946457 None:intergenic 55.0%
GGCAGCCGCGGCAAATGTCT+GGG - chr4_1:2946426-2946445 None:intergenic 65.0%
GGCAGCCGCGGCAAATGTCT+GGG - chr4_1:2946426-2946445 None:intergenic 65.0%
GGGCAGCCGCGGCAAATGTC+TGG - chr4_1:2946427-2946446 None:intergenic 70.0%
GGGCAGCCGCGGCAAATGTC+TGG - chr4_1:2946427-2946446 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 2944315 2946515 2944315 ID=Msa0529410;Name=Msa0529410
chr4_1 mRNA 2944315 2946515 2944315 ID=Msa0529410-mRNA-1;Parent=Msa0529410;Name=Msa0529410-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|0|0.14|0.71|0.33|0.57|7|194|208
chr4_1 exon 2944315 2944424 2944315 ID=Msa0529410-mRNA-1:exon:816;Parent=Msa0529410-mRNA-1
chr4_1 exon 2944803 2944867 2944803 ID=Msa0529410-mRNA-1:exon:817;Parent=Msa0529410-mRNA-1
chr4_1 exon 2944947 2945001 2944947 ID=Msa0529410-mRNA-1:exon:818;Parent=Msa0529410-mRNA-1
chr4_1 exon 2945095 2945178 2945095 ID=Msa0529410-mRNA-1:exon:819;Parent=Msa0529410-mRNA-1
chr4_1 exon 2945620 2945769 2945620 ID=Msa0529410-mRNA-1:exon:820;Parent=Msa0529410-mRNA-1
chr4_1 exon 2945857 2945987 2945857 ID=Msa0529410-mRNA-1:exon:821;Parent=Msa0529410-mRNA-1
chr4_1 exon 2946290 2946515 2946290 ID=Msa0529410-mRNA-1:exon:822;Parent=Msa0529410-mRNA-1
chr4_1 CDS 2944315 2944424 2944315 ID=Msa0529410-mRNA-1:cds;Parent=Msa0529410-mRNA-1
chr4_1 CDS 2944803 2944867 2944803 ID=Msa0529410-mRNA-1:cds;Parent=Msa0529410-mRNA-1
chr4_1 CDS 2944947 2945001 2944947 ID=Msa0529410-mRNA-1:cds;Parent=Msa0529410-mRNA-1
chr4_1 CDS 2945095 2945178 2945095 ID=Msa0529410-mRNA-1:cds;Parent=Msa0529410-mRNA-1
chr4_1 CDS 2945620 2945769 2945620 ID=Msa0529410-mRNA-1:cds;Parent=Msa0529410-mRNA-1
chr4_1 CDS 2945857 2945987 2945857 ID=Msa0529410-mRNA-1:cds;Parent=Msa0529410-mRNA-1
chr4_1 CDS 2946290 2946321 2946290 ID=Msa0529410-mRNA-1:cds;Parent=Msa0529410-mRNA-1
chr4_1 three_prime_UTR 2946322 2946515 2946322 ID=Msa0529410-mRNA-1:three_prime_utr;Parent=Msa0529410-mRNA-1
Gene Sequence

>Msa0529410

TTTATGGACTGCTGTGCTGGAATTGGTTTCTACAGATCAGTTTTAGTGTTAGATTTTTCTGAAATTGATTACTTTGAATTTGGATATGGGACCAAATTCATTGTCTTCGTGGATGTTGGCAGTAATATCGCATTGAAAATTCTGAAGCGTGTAGAAAAAGGTTGGGGTCAAGGAAATGGTTATTGGTTTGTTTTGTTGTTTGATTGTGGTTTTGGATACACTTTGATCATGAAACTGATAAGGTTGGTGAAGCAATATGGTGAAAGAAAATGGGCTCAAATAGCTGAGAAGTTAGAAGGAAGGGTTGGCAAGCAGATCGATCACTATCTTGGAAAAGAGCTTGTGGAAAATCTTTTTGTTCTCCGATTCTCAAATCTAATTTTTGAACCTTTGTGGTCGTGGCAATACATAAAAAATGTACAGTTGATATTCTCAGAAGATTTTGGTACTGAAGGACGTGGCAGGTACTTTGACAATTATGGTATAATTAGACATATTATGCAGAATCATCTACTTCAAATACTAGCCCTCTTTGCAATGGAAACCCCTGTTAGTTTGGATGTAGAGGACATTAGAAATGAAAAGGTCTCATATGGTCAAGGTTCTTCGTTCTATGAGACCGATTAA
Protein sequence

>Msa0529410

FMDCCAGIGFYRSVLVLDFSEIDYFEFGYGTKFIVFVDVGSNIALKILKRVEKGWGQGNGYWFVLLFDCGFGYTLIMKLIRLVKQYGERKWAQIAEKLEGRVGKQIDHYLGKELVENLFVLRFSNLIFEPLWSWQYIKNVQLIFSEDFGTEGRGRYFDNYGIIRHIMQNHLLQILALFAMETPVSLDVEDIRNEKVSYGQGSSFYETD*