AlfalfaGEDB | Msa0535420

M. sativa cultivar zhongmu-4 / Msa0535420

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535420	XP_013455021.2	86.901	313	39	1	1	313	1	311	0.0	572

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535420	sp\|Q8L9Y3\|ARR14_ARATH	38.403	263	121	5	7	232	4	262	1.79e-54	185

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535420	A0A396I0H3	84.395	314	46	2	1	313	1	312	0.0	546

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TFs/TRs:

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Gene ID	Type	Classification
Msa0535420	TF	GARP-ARR-B

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535420	MtrunA17_Chr4g0009851	84.395	314	46	2	1	313	1	312	0.0	546
Msa0535420	MtrunA17_Chr4g0009981	86.207	261	34	1	53	313	1	259	1.03e-169	471
Msa0535420	MtrunA17_Chr4g0009901	75.207	242	58	1	1	242	1	240	2.14e-128	367
Msa0535420	MtrunA17_Chr4g0011071	77.698	139	29	1	55	193	35	171	2.20e-73	224
Msa0535420	MtrunA17_Chr6g0471041	46.758	293	137	8	10	296	14	293	4.99e-71	226
Msa0535420	MtrunA17_Chr1g0161431	37.534	365	161	8	10	309	11	373	5.08e-68	223
Msa0535420	MtrunA17_Chr4g0009991	77.311	119	24	2	101	219	1	116	2.30e-57	181
Msa0535420	MtrunA17_Chr3g0123131	39.921	253	119	5	10	231	13	263	4.98e-57	193
Msa0535420	MtrunA17_Chr8g0376881	39.695	262	125	6	9	239	24	283	1.98e-56	191
Msa0535420	MtrunA17_Chr4g0067981	36.280	328	174	7	10	308	31	352	4.36e-55	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0535420	AT2G01760.1	38.403	263	121	5	7	232	4	262	1.81e-55	185
Msa0535420	AT1G67710.1	39.777	269	122	9	11	243	8	272	3.14e-53	182
Msa0535420	AT3G16857.1	35.714	308	140	9	10	259	33	340	2.80e-50	177
Msa0535420	AT3G16857.2	35.714	308	140	9	10	259	33	340	3.01e-50	177
Msa0535420	AT2G01760.2	35.052	291	120	7	7	232	4	290	1.19e-49	170
Msa0535420	AT4G31920.1	36.052	233	139	2	10	232	13	245	2.33e-45	162
Msa0535420	AT2G25180.1	37.917	240	125	5	10	226	13	251	1.43e-43	157
Msa0535420	AT3G16857.3	34.572	269	118	9	49	259	2	270	5.20e-39	145
Msa0535420	AT5G58080.1	37.349	249	132	6	10	236	14	260	6.74e-39	145
Msa0535420	AT1G67710.2	39.810	211	88	8	68	243	1	207	8.81e-36	134

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Find 56 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAACACTTGCAAAGA+AGG	0.435703	4_1:+11731702	Msa0535420:CDS
AAAAGTGTTGCTCATAATGT+TGG	0.490442	4_1:-11732513	None:intergenic
AAAATGAACCTGTAAATGGC+TGG	0.527802	4_1:-11731947	None:intergenic
AAGTCATAAACATCCATGTC+TGG	0.491105	4_1:-11731305	None:intergenic
AATAAAGAATATGAAATTGT+TGG	0.416766	4_1:+11731625	Msa0535420:CDS
AATAAATTCTTACTAATGAC+AGG	0.461511	4_1:-11731359	None:intergenic
AATGTGGGGCTTGCGAATAT+TGG	0.385194	4_1:+11731527	Msa0535420:CDS
AATTGCAATAACCTTGAGAC+CGG	0.581075	4_1:-11731027	None:intergenic
ACATATTGCCAGCCATTTAC+AGG	0.282271	4_1:+11731939	Msa0535420:CDS
ACATCTCCTCAATAGAGTTA+AGG	0.467190	4_1:-11731067	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATGAAAACTTGAATAAGGT+AGG	-	chr4_1:11731132-11731151	None:intergenic	30.0%
TTCAGTTACCAAATGTAGTA+TGG	+	chr4_1:11731214-11731233	Msa0535420:intron	30.0%
ATCATGAAGTCTATTGAATG+TGG	+	chr4_1:11731511-11731530	Msa0535420:CDS	30.0%
TCATGAAGTCTATTGAATGT+GGG	+	chr4_1:11731512-11731531	Msa0535420:CDS	30.0%
CATGTTGCAAGAATTAGAAA+CGG	+	chr4_1:11731592-11731611	Msa0535420:CDS	30.0%
GGTTCAAGAAAATAGAAAAC+AGG	+	chr4_1:11731657-11731676	Msa0535420:CDS	30.0%
AAAAGAAACACTTGCAAAGA+AGG	+	chr4_1:11731702-11731721	Msa0535420:CDS	30.0%
AGGAAAGAATCATACTATCA+AGG	-	chr4_1:11731789-11731808	None:intergenic	30.0%
AAAAGATAACTGACAAACCT+AGG	-	chr4_1:11732030-11732049	None:intergenic	30.0%
TGATTTACAACAACAGACTT+AGG	-	chr4_1:11732054-11732073	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	11730993	11732595	11730993	ID=Msa0535420;Name=Msa0535420
chr4_1	mRNA	11730993	11732595	11730993	ID=Msa0535420-mRNA-1;Parent=Msa0535420;Name=Msa0535420-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0\|0\|0\|1\|1\|1\|5\|0\|313
chr4_1	exon	11730993	11731110	11730993	ID=Msa0535420-mRNA-1:exon:3752;Parent=Msa0535420-mRNA-1
chr4_1	exon	11731219	11731368	11731219	ID=Msa0535420-mRNA-1:exon:3753;Parent=Msa0535420-mRNA-1
chr4_1	exon	11731482	11731793	11731482	ID=Msa0535420-mRNA-1:exon:3754;Parent=Msa0535420-mRNA-1
chr4_1	exon	11731884	11731960	11731884	ID=Msa0535420-mRNA-1:exon:3755;Parent=Msa0535420-mRNA-1
chr4_1	exon	11732311	11732595	11732311	ID=Msa0535420-mRNA-1:exon:3756;Parent=Msa0535420-mRNA-1
chr4_1	CDS	11730993	11731110	11730993	ID=Msa0535420-mRNA-1:cds;Parent=Msa0535420-mRNA-1
chr4_1	CDS	11731219	11731368	11731219	ID=Msa0535420-mRNA-1:cds;Parent=Msa0535420-mRNA-1
chr4_1	CDS	11731482	11731793	11731482	ID=Msa0535420-mRNA-1:cds;Parent=Msa0535420-mRNA-1
chr4_1	CDS	11731884	11731960	11731884	ID=Msa0535420-mRNA-1:cds;Parent=Msa0535420-mRNA-1
chr4_1	CDS	11732311	11732595	11732311	ID=Msa0535420-mRNA-1:cds;Parent=Msa0535420-mRNA-1

Gene Sequence

>Msa0535420

ATGGCAGTTTTCACTGACAACCCATCATTCCCAGCCGGTCTCAAGGTTATTGCAATTGATCATGACATCACTCTCCTTAACTCTATTGAGGAGATGTGTAATCGATATCATTATCAAGTTACCAAATGTAGTATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAAAAAGTTCTATCATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAAGAGGTCAAGATCATGTGGCAACATGTTGCAAGAATTAGAAACGGTTTGATTGAAAATAAAGAATATGAAATTGTTGGAAGCTCGCTGGTTCAAGAAAATAGAAAACAGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCACGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATATTGCCAGCCATTTACAGAAATACAGAAATCGGTTAAAGAGCTCAACAGAAGAGACAAAAGGTAGGATGAAAAAATCAAAGACGAGTCCTCTTGAAACAAAATTTGACTTAGAAGCTACTCACTCAATGCCGGAGCAGGACCAGAGTTTTCTCCTGAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACGACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCAGACTTGCCCAACTTGTTCCCATATGTTAATGATGATTTATCATCACTTGTGTGGTGA

Protein sequence

>Msa0535420

MAVFTDNPSFPAGLKVIAIDHDITLLNSIEEMCNRYHYQVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLNIPVIMMAVDSTKSSIMKSIECGACEYWTKPLVEKEVKIMWQHVARIRNGLIENKEYEIVGSSLVQENRKQGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHIASHLQKYRNRLKSSTEETKGRMKKSKTSPLETKFDLEATHSMPEQDQSFLLNSATQCRDNCDAQRHWVEIGESNIMSNTFSDLPNLFPYVNDDLSSLVW*