AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0541670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541670 MtrunA17_Chr8g0385911 95.017 301 9 3 1 299 1 297 0.0 508
Msa0541670 MtrunA17_Chr3g0131771 57.009 321 105 10 1 300 1 309 8.55e-99 293
Msa0541670 MtrunA17_Chr7g0234761 51.596 188 70 7 78 250 236 417 2.08e-46 163
Msa0541670 MtrunA17_Chr1g0152131 47.778 180 83 4 61 234 170 344 1.64e-44 156
Msa0541670 MtrunA17_Chr8g0365941 53.988 163 61 3 73 227 228 384 1.13e-43 155
Msa0541670 MtrunA17_Chr5g0415291 62.185 119 44 1 100 218 66 183 2.98e-42 145
Msa0541670 MtrunA17_Chr8g0339091 45.494 233 103 8 24 236 149 377 4.28e-42 151
Msa0541670 MtrunA17_Chr3g0144641 55.696 158 58 5 81 227 114 270 1.17e-41 148
Msa0541670 MtrunA17_Chr4g0035101 51.955 179 71 6 60 227 48 222 1.96e-41 146
Msa0541670 MtrunA17_Chr8g0363921 64.103 117 33 1 111 218 106 222 5.64e-41 143
Msa0541670 MtrunA17_Chr5g0421111 47.872 188 71 6 66 239 281 455 1.09e-40 148
Msa0541670 MtrunA17_Chr1g0174161 71.739 92 23 2 136 227 151 239 1.17e-39 141
Msa0541670 MtrunA17_Chr7g0258161 58.730 126 41 4 138 258 166 285 3.18e-38 137
Msa0541670 MtrunA17_Chr6g0484271 48.408 157 70 3 75 231 93 238 7.18e-36 130
Msa0541670 MtrunA17_Chr4g0074161 57.831 83 30 2 147 225 206 287 1.72e-21 93.6
Msa0541670 MtrunA17_Chr1g0190731 65.152 66 22 1 147 212 193 257 1.52e-19 87.4
Msa0541670 MtrunA17_Chr4g0014771 55.385 65 29 0 147 211 118 182 8.65e-16 74.3
Msa0541670 MtrunA17_Chr4g0032951 56.923 65 28 0 147 211 355 419 2.68e-15 75.9
Msa0541670 MtrunA17_Chr1g0182791 47.297 74 37 2 149 222 319 390 2.98e-14 72.8
Msa0541670 MtrunA17_Chr1g0193321 38.202 89 54 1 124 212 439 526 5.02e-14 72.4
Msa0541670 MtrunA17_Chr1g0184841 38.732 142 72 3 99 226 62 202 5.63e-14 70.9
Msa0541670 MtrunA17_Chr1g0174271 49.296 71 36 0 147 217 298 368 5.70e-14 71.6
Msa0541670 MtrunA17_Chr7g0258181 49.275 69 35 0 147 215 243 311 1.10e-13 70.5
Msa0541670 MtrunA17_Chr2g0329041 50.769 65 32 0 145 209 330 394 1.71e-13 70.5
Msa0541670 MtrunA17_Chr5g0402031 38.596 114 62 4 116 222 68 180 7.84e-13 68.2
Msa0541670 MtrunA17_Chr3g0087771 49.231 65 33 0 147 211 322 386 2.75e-12 66.6
Msa0541670 MtrunA17_Chr7g0262691 30.818 159 89 5 107 253 214 363 8.84e-12 65.5
Msa0541670 MtrunA17_Chr1g0152651 49.231 65 32 1 147 211 274 337 1.07e-11 64.7
Msa0541670 MtrunA17_Chr3g0095391 35.294 119 51 5 149 255 311 415 2.19e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0541670 AT5G50915.3 45.565 248 96 7 1 234 1 223 4.21e-55 180
Msa0541670 AT5G50915.4 45.565 248 96 7 1 234 1 223 4.21e-55 180
Msa0541670 AT5G50915.1 44.215 242 108 5 1 234 1 223 1.09e-54 179
Msa0541670 AT5G50915.2 44.215 242 108 5 1 234 1 223 1.09e-54 179
Msa0541670 AT3G23690.1 51.366 183 77 5 104 280 148 324 7.29e-47 161
Msa0541670 AT1G68920.3 50.000 198 91 6 56 251 211 402 4.28e-46 162
Msa0541670 AT1G68920.1 50.000 204 83 7 56 251 211 403 7.56e-46 161
Msa0541670 AT1G68920.4 50.000 204 83 7 56 251 211 403 7.56e-46 161
Msa0541670 AT1G68920.2 50.000 204 83 7 56 251 211 403 7.56e-46 161
Msa0541670 AT1G26260.3 45.024 211 80 7 47 252 89 268 3.69e-45 156
Msa0541670 AT1G26260.5 45.024 211 80 7 47 252 118 297 5.18e-45 157
Msa0541670 AT3G07340.2 60.544 147 50 3 81 227 199 337 5.19e-45 158
Msa0541670 AT1G10120.2 38.462 260 126 8 6 234 34 290 5.48e-45 156
Msa0541670 AT1G10120.1 38.462 260 126 8 6 234 34 290 5.48e-45 156
Msa0541670 AT3G07340.1 60.544 147 50 3 81 227 199 337 5.74e-45 159
Msa0541670 AT1G26260.4 45.024 211 80 7 47 252 140 319 7.00e-45 157
Msa0541670 AT1G26260.1 45.024 211 80 7 47 252 140 319 7.00e-45 157
Msa0541670 AT1G26260.6 45.024 211 80 7 47 252 140 319 7.00e-45 157
Msa0541670 AT1G26260.2 45.024 211 80 7 47 252 140 319 7.00e-45 157
Msa0541670 AT5G48560.2 50.838 179 76 2 61 227 203 381 2.41e-44 157
Msa0541670 AT5G48560.1 50.838 179 76 2 61 227 203 381 4.35e-44 157
Msa0541670 AT1G59640.1 57.343 143 59 1 76 218 67 207 3.24e-43 149
Msa0541670 AT1G59640.2 56.552 145 61 1 74 218 65 207 4.90e-43 151
Msa0541670 AT4G34530.1 46.725 229 92 9 12 227 40 251 5.98e-42 148
Msa0541670 AT5G62610.2 53.425 146 51 3 105 233 101 246 2.13e-39 139
Msa0541670 AT5G62610.1 53.425 146 51 3 105 233 101 246 2.13e-39 139
Msa0541670 AT3G57800.2 71.875 96 22 3 138 233 197 287 8.25e-39 140
Msa0541670 AT2G42300.4 60.000 120 47 1 108 227 146 264 1.97e-38 137
Msa0541670 AT2G42300.1 60.000 120 47 1 108 227 146 264 7.38e-38 137
Msa0541670 AT2G18300.2 50.667 150 72 1 70 219 110 257 3.12e-37 135
Msa0541670 AT2G18300.1 50.667 150 72 1 70 219 110 257 3.65e-37 135
Msa0541670 AT2G18300.3 50.667 150 72 1 70 219 110 257 5.66e-37 135
Msa0541670 AT4G36540.1 58.678 121 39 3 106 219 97 213 8.10e-37 133
Msa0541670 AT4G36540.2 58.678 121 39 3 106 219 97 213 8.24e-37 133
Msa0541670 AT1G18400.2 67.816 87 27 1 145 231 143 228 3.25e-35 127
Msa0541670 AT1G18400.1 67.816 87 27 1 145 231 143 228 3.65e-35 128
Msa0541670 AT1G73830.2 67.442 86 27 1 146 231 146 230 4.59e-34 125
Msa0541670 AT1G73830.1 67.442 86 27 1 146 231 146 230 4.60e-34 125
Msa0541670 AT1G25330.1 61.053 95 32 1 141 235 98 187 1.38e-33 123
Msa0541670 AT3G57800.1 48.951 143 21 4 138 233 197 334 1.75e-30 119
Msa0541670 AT5G58010.1 50.962 104 38 3 147 246 99 193 1.40e-21 92.8
Msa0541670 AT2G24260.2 62.500 72 26 1 141 212 29 99 3.13e-21 90.9
Msa0541670 AT4G30980.1 68.182 66 20 1 147 212 130 194 8.92e-21 90.9
Msa0541670 AT4G30980.2 68.182 66 20 1 147 212 178 242 1.16e-20 91.3
Msa0541670 AT2G24260.1 66.667 66 21 1 147 212 138 202 1.86e-20 90.5
Msa0541670 AT2G42300.5 81.395 43 8 0 144 186 182 224 4.45e-19 84.3
Msa0541670 AT2G42300.3 81.395 43 8 0 144 186 182 224 4.45e-19 84.3
Msa0541670 AT2G42300.2 81.395 43 8 0 144 186 182 224 5.23e-19 84.3
Msa0541670 AT1G09530.3 41.573 89 48 2 124 212 317 401 7.81e-14 72.0
Msa0541670 AT1G09530.2 41.573 89 48 2 124 212 317 401 7.81e-14 72.0
Msa0541670 AT1G09530.5 41.573 89 48 2 124 212 317 401 7.81e-14 72.0
Msa0541670 AT1G09530.1 41.573 89 48 2 124 212 317 401 7.81e-14 72.0
Msa0541670 AT1G09530.4 41.573 89 48 2 124 212 317 401 7.81e-14 72.0
Msa0541670 AT1G09530.6 41.573 89 48 2 124 212 317 401 7.81e-14 72.0
Msa0541670 AT4G28800.1 45.833 72 38 1 150 221 252 322 1.01e-13 71.6
Msa0541670 AT4G28815.2 38.318 107 56 4 150 253 71 170 1.13e-13 69.7
Msa0541670 AT1G35460.1 50.769 65 32 0 147 211 181 245 1.65e-13 69.7
Msa0541670 AT1G51140.1 54.688 64 29 0 147 210 304 367 2.77e-13 70.1
Msa0541670 AT2G42280.1 50.000 64 32 0 147 210 279 342 4.01e-13 69.3
Msa0541670 AT4G09180.1 49.254 67 34 0 145 211 182 248 4.40e-13 68.6
Msa0541670 AT4G28815.1 38.318 107 56 4 150 253 147 246 4.62e-13 68.9
Msa0541670 AT2G20180.7 49.206 63 31 1 150 212 210 271 4.71e-13 69.3
Msa0541670 AT2G20180.1 49.206 63 31 1 150 212 210 271 4.71e-13 69.3
Msa0541670 AT2G20180.5 49.206 63 31 1 150 212 210 271 4.71e-13 69.3
Msa0541670 AT2G20180.4 49.206 63 31 1 150 212 281 342 5.03e-13 69.7
Msa0541670 AT2G20180.6 49.206 63 31 1 150 212 281 342 5.03e-13 69.7
Msa0541670 AT2G20180.2 49.206 63 31 1 150 212 281 342 5.03e-13 69.7
Msa0541670 AT2G20180.3 49.206 63 31 1 150 212 281 342 5.03e-13 69.7
Msa0541670 AT2G42280.3 51.515 66 30 1 147 210 279 344 6.49e-13 68.9
Msa0541670 AT4G28790.1 41.892 74 42 1 149 222 273 345 1.59e-12 67.8
Msa0541670 AT4G02590.3 50.000 84 37 2 147 226 83 165 1.66e-12 66.6
Msa0541670 AT4G28800.2 50.820 61 29 1 150 210 264 323 1.95e-12 67.8
Msa0541670 AT4G02590.2 50.000 84 37 2 147 226 146 228 2.39e-12 66.6
Msa0541670 AT4G02590.1 50.000 84 37 2 147 226 146 228 2.39e-12 66.6
Msa0541670 AT1G03040.2 59.091 66 26 1 147 212 139 203 9.03e-12 65.1
Msa0541670 AT1G03040.1 59.091 66 26 1 147 212 144 208 1.05e-11 64.7
Msa0541670 AT1G03040.3 59.091 66 26 1 147 212 144 208 1.59e-11 64.7
Msa0541670 AT2G20180.8 49.153 59 29 1 150 208 281 338 1.91e-11 64.3
Msa0541670 AT4G33880.1 45.783 83 42 3 147 228 266 346 2.00e-11 64.3
Msa0541670 AT2G43140.1 43.590 78 42 1 135 210 120 197 2.35e-11 62.8
Msa0541670 AT2G14760.1 44.186 86 43 3 147 228 237 321 2.93e-11 63.5
Msa0541670 AT2G43140.2 43.590 78 42 1 135 210 217 294 2.94e-11 63.5
Msa0541670 AT4G00050.2 44.595 74 40 1 138 211 96 168 8.37e-11 62.0

Find 134 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACACTGGTTTCTGTTTC+TGG 0.033103 4_1:-25660733 None:intergenic
GTGAAATTGATATTAATTTA+AGG 0.098761 4_1:-25663257 None:intergenic
ATGTTATATGTGTAAATTAT+TGG 0.172476 4_1:+25663799 Msa0541670:three_prime_UTR
AGCACACTGATGGACCATTT+TGG 0.191470 4_1:+25663173 Msa0541670:three_prime_UTR
GTTCATATGTCACTTTAATT+TGG 0.207995 4_1:-25663565 None:intergenic
TTAATTGTTGAAGTTATTAA+AGG 0.229710 4_1:-25662697 None:intergenic
CACTCATTCTGCTACTTGTT+TGG 0.256067 4_1:-25663445 None:intergenic
ATAAAGTGTAGCAAAATTAA+TGG 0.267100 4_1:+25663595 Msa0541670:three_prime_UTR
GATCAAAGTACTTTATGTTT+TGG 0.272122 4_1:-25663114 None:intergenic
TGCTAATAATGACTATGTTT+TGG 0.277470 4_1:+25662468 Msa0541670:CDS
GGTTATTGAGAAATGATTAA+TGG 0.303104 4_1:+25663890 Msa0541670:three_prime_UTR
TCTGCATGGGAATGAGTTAA+TGG 0.307353 4_1:+25660565 Msa0541670:five_prime_UTR
TTTCATATTAAATAGGTAAC+TGG 0.313052 4_1:+25661984 Msa0541670:intron
TTTATTGCAAAATGTTAATC+AGG 0.314238 4_1:-25663059 None:intergenic
TATATGATTATAAATATAAT+TGG 0.315263 4_1:+25662571 Msa0541670:three_prime_UTR
TTTGAGTAGCCCCTTGTCTA+AGG 0.324771 4_1:+25661086 Msa0541670:CDS
ATCAACAAGTCTCTACATTA+TGG 0.325328 4_1:+25662786 Msa0541670:three_prime_UTR
AGGAAGAGAAGTGTTTATAT+TGG 0.325944 4_1:-25660863 None:intergenic
TACTCAGAAAGTTACTTCTA+AGG 0.338181 4_1:+25661032 Msa0541670:CDS
CAACATGTTTGTAGACTTGT+AGG 0.350934 4_1:-25663288 None:intergenic
TATCAAAATCTTATGATCTA+AGG 0.351276 4_1:-25662547 None:intergenic
CTGGTTTCTGTTTCTGGTGT+TGG 0.352379 4_1:-25660727 None:intergenic
CAAGAAAGGGTTGTTGGTAT+TGG 0.354267 4_1:-25660822 None:intergenic
TGTCCTTGAGTTGGTATTAG+AGG 0.362112 4_1:-25662978 None:intergenic
CGTCTGAATTTGTTATCAAA+TGG 0.364587 4_1:+25662888 Msa0541670:three_prime_UTR
CAAAAGCTTCTTAATCCATA+TGG 0.364693 4_1:+25663214 Msa0541670:three_prime_UTR
AATCCTAGAGATATCATATC+AGG 0.380720 4_1:+25663758 Msa0541670:three_prime_UTR
ATTGTAAAGTCAAAGAAGAT+TGG 0.383533 4_1:-25662191 None:intergenic
TATTTCCTCACCTGATGATC+TGG 0.386280 4_1:-25662239 None:intergenic
TCATATCTTGATAGCTCATA+TGG 0.415510 4_1:+25660786 Msa0541670:exon
CTTCTTAATCCATATGGATT+CGG 0.417843 4_1:+25663220 Msa0541670:three_prime_UTR
TGATTCTTGTGTTGTGGTGG+TGG 0.421917 4_1:+25663831 Msa0541670:three_prime_UTR
AACTTGTGCCTGGCTGTGAT+AGG 0.422109 4_1:+25661671 Msa0541670:CDS
TAATTGTTGAAGTTATTAAA+GGG 0.424789 4_1:-25662696 None:intergenic
TGATCGATGGTTTGAATGAA+GGG 0.440167 4_1:+25663508 Msa0541670:three_prime_UTR
GGACTCTTGAAAATGATAGA+AGG 0.440666 4_1:-25660761 None:intergenic
TAGCCACAGCCTTGCTGAAA+GGG 0.444848 4_1:+25661362 Msa0541670:CDS
ACATGTGGTATTGGTGTTGA+TGG 0.451696 4_1:-25662382 None:intergenic
CACAATGGTACATGTGGTAT+TGG 0.457363 4_1:-25662391 None:intergenic
ATGATCGATGGTTTGAATGA+AGG 0.461186 4_1:+25663507 Msa0541670:three_prime_UTR
TAGCAGAATGAGTGAAAAGA+AGG 0.462032 4_1:+25663454 Msa0541670:three_prime_UTR
AGTAGTGGTGAGGGAATGAA+AGG 0.463556 4_1:+25661237 Msa0541670:CDS
GCCACAATATGTTTATATAT+AGG 0.464119 4_1:+25660618 Msa0541670:five_prime_UTR
TGGTCTTCTACGTCCCAAAA+TGG 0.467780 4_1:-25663187 None:intergenic
CATATGTCACTTTAATTTGG+TGG 0.468912 4_1:-25663562 None:intergenic
TTGGCTCATGAGATAATGTC+AGG 0.470562 4_1:+25662590 Msa0541670:three_prime_UTR
TTCTAAATATAAGTCCAATA+CGG 0.470688 4_1:+25662668 Msa0541670:three_prime_UTR
AAGGGATTCTGTTCTATGCT+TGG 0.472431 4_1:+25663526 Msa0541670:three_prime_UTR
GTGTTGTTGCCGAATCCATA+TGG 0.475225 4_1:-25663229 None:intergenic
GGTAGTGGACAATCTTGAAA+AGG 0.476116 4_1:+25660999 Msa0541670:CDS
AGTCAACAAGAAAGGGTTGT+TGG 0.476660 4_1:-25660828 None:intergenic
AATATTGAAGAAGTTCCAAC+TGG 0.478486 4_1:+25661300 Msa0541670:CDS
GACCCCTTCTTGCTCTGACA+TGG 0.481977 4_1:-25661329 None:intergenic
CAAAAGAAGAGTAGTGGTGA+GGG 0.482696 4_1:+25661228 Msa0541670:CDS
CTTGGTTAGACCAGATCATC+AGG 0.485638 4_1:+25662229 Msa0541670:CDS
GTCCTTGAGTTGGTATTAGA+GGG 0.486243 4_1:-25662977 None:intergenic
AATGTAGAGACTTGTTGATA+AGG 0.492518 4_1:-25662782 None:intergenic
CCGGTCAAATATACATGTAC+TGG 0.495516 4_1:+25660538 None:intergenic
TTGTAGGATATCTCAAAAGA+AGG 0.497168 4_1:+25661192 Msa0541670:intron
GGACTATAACGATGCTGAGT+TGG 0.497355 4_1:+25663030 Msa0541670:three_prime_UTR
GTATGTAGAGTCAACAAGAA+AGG 0.497449 4_1:-25660836 None:intergenic
TCACTTTAATTTGGTGGACT+AGG 0.499634 4_1:-25663556 None:intergenic
ATTAAAGGGAAGTACCGTAT+TGG 0.503741 4_1:-25662682 None:intergenic
AGCAAAAGGTGTGGCATGGT+TGG 0.509890 4_1:-25662419 None:intergenic
CATGTACTGGTCTCTCTGCA+TGG 0.516443 4_1:+25660551 None:intergenic
TGCTGAATCATCCATGGTAG+TGG 0.516620 4_1:+25660984 Msa0541670:CDS
TGTCAGAGCAAGAAGGGGTC+AGG 0.517190 4_1:+25661332 Msa0541670:CDS
ATTCTGTTCTATGCTTGGCA+TGG 0.517527 4_1:+25663531 Msa0541670:three_prime_UTR
AAAACATAGTCATTATTAGC+AGG 0.519599 4_1:-25662466 None:intergenic
AATTAGATTTGTCCTTGAGT+TGG 0.521907 4_1:-25662987 None:intergenic
CAAGATTGATTCTTGTGTTG+TGG 0.522292 4_1:+25663825 Msa0541670:three_prime_UTR
ATATTGCAGCAACTTGTGCC+TGG 0.524669 4_1:+25661661 Msa0541670:CDS
TGTAGCAAAATTAATGGATG+AGG 0.525012 4_1:+25663601 Msa0541670:three_prime_UTR
TATGTAGAGTCAACAAGAAA+GGG 0.525797 4_1:-25660835 None:intergenic
ATCAAATTTACCTTAGACAA+GGG 0.526806 4_1:-25661096 None:intergenic
ATAGCCACAGCCTTGCTGAA+AGG 0.535109 4_1:+25661361 Msa0541670:CDS
GGACTATAACGATGTTGAGT+TGG 0.535328 4_1:-25662956 None:intergenic
ATACCCTTTCAGCAAGGCTG+TGG 0.536175 4_1:-25661365 None:intergenic
GAGGTCAAATACCTTCTTTG+AGG 0.536244 4_1:+25662522 Msa0541670:CDS
CCTCCTGATATGATATCTCT+AGG 0.539860 4_1:-25663761 None:intergenic
CTTGAAAATGATAGAAGGAA+AGG 0.541454 4_1:-25660756 None:intergenic
AATCTTCTTTGACTTTACAA+TGG 0.545151 4_1:+25662193 Msa0541670:CDS
AACATGTTTGTAGACTTGTA+GGG 0.548178 4_1:-25663287 None:intergenic
TTGGACTCAGTCGCAAAAGT+TGG 0.549192 4_1:-25660667 None:intergenic
GATCTAAGGTACCTCAAAGA+AGG 0.549335 4_1:-25662533 None:intergenic
CATAGTCATTATTAGCAGGA+TGG 0.551687 4_1:-25662462 None:intergenic
GCAGAAGCAGGTATCAGTGT+TGG 0.552063 4_1:-25660686 None:intergenic
GTGGTAACTGTGTCAGCAAA+AGG 0.552676 4_1:-25662433 None:intergenic
TATCCATGTCAGAGCAAGAA+GGG 0.554936 4_1:+25661326 Msa0541670:CDS
TGTAGGATATCTCAAAAGAA+GGG 0.556436 4_1:+25661193 Msa0541670:intron
GTAGTGGACAATCTTGAAAA+GGG 0.561287 4_1:+25661000 Msa0541670:CDS
AGCAGAATGAGTGAAAAGAA+GGG 0.561330 4_1:+25663455 Msa0541670:three_prime_UTR
TAATGGATGAGGAAGCCAAA+AGG 0.562677 4_1:+25663612 Msa0541670:three_prime_UTR
TTCAAGATTGTCCACTACCA+TGG 0.563562 4_1:-25660995 None:intergenic
TTTGCTGACACAGTTACCAC+AGG 0.568907 4_1:+25662436 Msa0541670:CDS
TGATACTCAAAAGCAGAAGC+AGG 0.569542 4_1:-25660698 None:intergenic
ACTTGTGCCTGGCTGTGATA+GGG 0.572729 4_1:+25661672 Msa0541670:CDS
ACCTATATATAAACATATTG+TGG 0.573339 4_1:-25660619 None:intergenic
GATTGATTCTTGTGTTGTGG+TGG 0.573353 4_1:+25663828 Msa0541670:three_prime_UTR
AATGGACCTAGACACACTCT+TGG 0.574526 4_1:+25662211 Msa0541670:CDS
AAGATGAATCTTGATATTCA+AGG 0.575266 4_1:-25662863 None:intergenic
ACAAAAGAAGAGTAGTGGTG+AGG 0.575865 4_1:+25661227 Msa0541670:CDS
AAATATTATTATTAGAACAA+AGG 0.577853 4_1:-25663677 None:intergenic
AACTGGAAAGGCCCTTGTGT+TGG 0.580713 4_1:+25662001 Msa0541670:CDS
CATCAAATTTACCTTAGACA+AGG 0.584025 4_1:-25661097 None:intergenic
ATGTACTGGTCTCTCTGCAT+GGG 0.586524 4_1:+25660552 None:intergenic
TGATTATTTCATCCAACACA+AGG 0.586530 4_1:-25662013 None:intergenic
AGAGATATCATATCAGGAGG+AGG 0.593669 4_1:+25663764 Msa0541670:three_prime_UTR
TGGTGAGGGAATGAAAGGAG+AGG 0.596090 4_1:+25661242 Msa0541670:CDS
GTCCTTACAAAATCAAGTAG+AGG 0.597808 4_1:+25662046 Msa0541670:CDS
CCATGGATGATTCAGCACTC+TGG 0.598387 4_1:-25660978 None:intergenic
TGGCTCATGAGATAATGTCA+GGG 0.608292 4_1:+25662591 Msa0541670:three_prime_UTR
GTCCCTCTAATACCAACTCA+AGG 0.609451 4_1:+25662975 Msa0541670:three_prime_UTR
GTCTAACCAAGAGTGTGTCT+AGG 0.610495 4_1:-25662217 None:intergenic
ATATCCATGTCAGAGCAAGA+AGG 0.615381 4_1:+25661325 Msa0541670:CDS
CCAGAGTGCTGAATCATCCA+TGG 0.615529 4_1:+25660978 Msa0541670:CDS
TATTAAATAGGTAACTGGAA+AGG 0.623233 4_1:+25661989 Msa0541670:intron
AGACTCACCCTATCACAGCC+AGG 0.626341 4_1:-25661679 None:intergenic
GATTATTTCATCCAACACAA+GGG 0.628005 4_1:-25662012 None:intergenic
ATAGAAGGAAAGGCAAACAC+TGG 0.630513 4_1:-25660746 None:intergenic
TCAAATTTACCTTAGACAAG+GGG 0.631795 4_1:-25661095 None:intergenic
TATTGCATACCCTTTCAGCA+AGG 0.648025 4_1:-25661371 None:intergenic
CTGACATGGATATAACCAGT+TGG 0.650898 4_1:-25661315 None:intergenic
CCTAGAGATATCATATCAGG+AGG 0.658421 4_1:+25663761 Msa0541670:three_prime_UTR
ATGAGCTATCAAGATATGAG+AGG 0.660125 4_1:-25660782 None:intergenic
ATCCATGTCAGAGCAAGAAG+GGG 0.665171 4_1:+25661327 Msa0541670:CDS
GGCTCATGAGATAATGTCAG+GGG 0.666557 4_1:+25662592 Msa0541670:three_prime_UTR
GAGCTGCACAATGGTACATG+TGG 0.691981 4_1:-25662397 None:intergenic
TGTCAGCAAAAGGTGTGGCA+TGG 0.694030 4_1:-25662423 None:intergenic
ATGAATCTTGATATTCAAGG+AGG 0.697991 4_1:-25662860 None:intergenic
AACTGTGTCAGCAAAAGGTG+TGG 0.699759 4_1:-25662428 None:intergenic
GCATGGTTGGAGCTGCACAA+TGG 0.724748 4_1:-25662406 None:intergenic
ATTAGCAGGATGGAAGCCTG+TGG 0.733746 4_1:-25662452 None:intergenic
GGTCTGTCACATAAACTCAG+AGG 0.737839 4_1:-25660646 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTATTATTAGAACAA+AGG - chr4_1:25663680-25663699 None:intergenic 10.0%
!! ATAGAAACATATTCAAAAAA+GGG - chr4_1:25661135-25661154 None:intergenic 15.0%
!! TATAGAAACATATTCAAAAA+AGG - chr4_1:25661136-25661155 None:intergenic 15.0%
!! AATAAATGTTGCAAAAAAAT+TGG + chr4_1:25661598-25661617 Msa0541670:intron 15.0%
!!! AAAAGTACACAAAATATTAA+AGG - chr4_1:25661710-25661729 None:intergenic 15.0%
!! TACAATGAAAATTATTACAA+GGG - chr4_1:25661826-25661845 None:intergenic 15.0%
!!! AAAGTACAACTTTTATATTT+TGG - chr4_1:25662080-25662099 None:intergenic 15.0%
!! ATATAGAAGTTTATAATGTT+AGG - chr4_1:25662130-25662149 None:intergenic 15.0%
!! TAATTGTTGAAGTTATTAAA+GGG - chr4_1:25662699-25662718 None:intergenic 15.0%
!! TTAATTGTTGAAGTTATTAA+AGG - chr4_1:25662700-25662719 None:intergenic 15.0%
!! TTTACTCATTAATTATTGTT+TGG - chr4_1:25662741-25662760 None:intergenic 15.0%
!! GTGAAATTGATATTAATTTA+AGG - chr4_1:25663260-25663279 None:intergenic 15.0%
!! ATAATAAATATTTCTTTGCA+AGG - chr4_1:25663331-25663350 None:intergenic 15.0%
!! ACAAAAGATTAAAATACAAA+GGG - chr4_1:25663388-25663407 None:intergenic 15.0%
!! TACAAAAGATTAAAATACAA+AGG - chr4_1:25663389-25663408 None:intergenic 15.0%
!!! TTGTATTTTAATCTTTTGTA+AGG + chr4_1:25663389-25663408 Msa0541670:intron 15.0%
!! ATGTTATATGTGTAAATTAT+TGG + chr4_1:25663799-25663818 Msa0541670:three_prime_UTR 15.0%
!! ACCTATATATAAACATATTG+TGG - chr4_1:25660622-25660641 None:intergenic 20.0%
!!! TTATTGATTTTGTTACTTTG+TGG + chr4_1:25661166-25661185 Msa0541670:intron 20.0%
!!! TTTTAAAATTACTACACTGA+TGG - chr4_1:25661456-25661475 None:intergenic 20.0%
!! ATTACAATTTACAACTGAAA+AGG + chr4_1:25661538-25661557 Msa0541670:intron 20.0%
!! CTACAATGAAAATTATTACA+AGG - chr4_1:25661827-25661846 None:intergenic 20.0%
!!! GATTTGGTTTCATATTAAAT+AGG + chr4_1:25661977-25661996 Msa0541670:intron 20.0%
!! TTTCATATTAAATAGGTAAC+TGG + chr4_1:25661984-25662003 Msa0541670:intron 20.0%
!! TATCAAAATCTTATGATCTA+AGG - chr4_1:25662550-25662569 None:intergenic 20.0%
!! TTCTAAATATAAGTCCAATA+CGG + chr4_1:25662668-25662687 Msa0541670:three_prime_UTR 20.0%
!!! ATTAACATTTTGCAATTTCA+AGG - chr4_1:25662827-25662846 None:intergenic 20.0%
!!! TTAACATTTTGCAATTTTAG+AGG + chr4_1:25663008-25663027 Msa0541670:three_prime_UTR 20.0%
!!! TAACATTTTGCAATTTTAGA+GGG + chr4_1:25663009-25663028 Msa0541670:three_prime_UTR 20.0%
!! TTTATTGCAAAATGTTAATC+AGG - chr4_1:25663062-25663081 None:intergenic 20.0%
!!! TTTTGGTAAAATGACTAAAT+TGG - chr4_1:25663100-25663119 None:intergenic 20.0%
!!! ATATGCTTTTCATCATAATA+AGG - chr4_1:25663144-25663163 None:intergenic 20.0%
!! ATAAATCAAAAAATGATCGA+TGG + chr4_1:25663495-25663514 Msa0541670:three_prime_UTR 20.0%
!! ATAAAGTGTAGCAAAATTAA+TGG + chr4_1:25663595-25663614 Msa0541670:three_prime_UTR 20.0%
! GCCACAATATGTTTATATAT+AGG + chr4_1:25660618-25660637 Msa0541670:five_prime_UTR 25.0%
! TGATTATGAAAAAGATGAGA+AGG - chr4_1:25660886-25660905 None:intergenic 25.0%
! ATCAAATTTACCTTAGACAA+GGG - chr4_1:25661099-25661118 None:intergenic 25.0%
! AAAAAACAAAAGAAGAGTAG+TGG + chr4_1:25661222-25661241 Msa0541670:CDS 25.0%
! TTGCAAAAAAATTGGATTTC+AGG + chr4_1:25661606-25661625 Msa0541670:intron 25.0%
!!! CTATTTCTATAGCATTGATA+TGG + chr4_1:25661767-25661786 Msa0541670:intron 25.0%
!!! TATAGCATTGATATGGATAT+CGG + chr4_1:25661774-25661793 Msa0541670:intron 25.0%
!!! TTGAAGTATCGGTTTTAAAT+AGG + chr4_1:25661920-25661939 Msa0541670:intron 25.0%
! TATTAAATAGGTAACTGGAA+AGG + chr4_1:25661989-25662008 Msa0541670:intron 25.0%
! ATTGTAAAGTCAAAGAAGAT+TGG - chr4_1:25662194-25662213 None:intergenic 25.0%
!! AATCTTCTTTGACTTTACAA+TGG + chr4_1:25662193-25662212 Msa0541670:CDS 25.0%
! AAAACATAGTCATTATTAGC+AGG - chr4_1:25662469-25662488 None:intergenic 25.0%
!! TGCTAATAATGACTATGTTT+TGG + chr4_1:25662468-25662487 Msa0541670:CDS 25.0%
!! CAACTTCAATTTTTCTTCAA+GGG + chr4_1:25662497-25662516 Msa0541670:CDS 25.0%
! AAGATGAATCTTGATATTCA+AGG - chr4_1:25662866-25662885 None:intergenic 25.0%
!!! GATCAAAGTACTTTATGTTT+TGG - chr4_1:25663117-25663136 None:intergenic 25.0%
!!! TCTTTTCTAGTATAGATAGA+AGG + chr4_1:25663417-25663436 Msa0541670:three_prime_UTR 25.0%
!!! GTTCATATGTCACTTTAATT+TGG - chr4_1:25663568-25663587 None:intergenic 25.0%
!!! AAGATATGGTCTTATTTTGT+CGG - chr4_1:25663648-25663667 None:intergenic 25.0%
! AAAGGATCTAGAAAAAGATA+TGG - chr4_1:25663662-25663681 None:intergenic 25.0%
! GGTTATTGAGAAATGATTAA+TGG + chr4_1:25663890-25663909 Msa0541670:three_prime_UTR 25.0%
CTTGAAAATGATAGAAGGAA+AGG - chr4_1:25660759-25660778 None:intergenic 30.0%
! TCATATCTTGATAGCTCATA+TGG + chr4_1:25660786-25660805 Msa0541670:exon 30.0%
TATGTAGAGTCAACAAGAAA+GGG - chr4_1:25660838-25660857 None:intergenic 30.0%
!! AGGAAGAGAAGTGTTTATAT+TGG - chr4_1:25660866-25660885 None:intergenic 30.0%
TACTCAGAAAGTTACTTCTA+AGG + chr4_1:25661032-25661051 Msa0541670:CDS 30.0%
TCAAATTTACCTTAGACAAG+GGG - chr4_1:25661098-25661117 None:intergenic 30.0%
CATCAAATTTACCTTAGACA+AGG - chr4_1:25661100-25661119 None:intergenic 30.0%
!! TGTTACTTTGTGGTATTTGT+AGG + chr4_1:25661176-25661195 Msa0541670:intron 30.0%
TTGTAGGATATCTCAAAAGA+AGG + chr4_1:25661192-25661211 Msa0541670:intron 30.0%
TGTAGGATATCTCAAAAGAA+GGG + chr4_1:25661193-25661212 Msa0541670:intron 30.0%
GAAGAAAAAAGAAGAGAAGA+AGG + chr4_1:25661266-25661285 Msa0541670:CDS 30.0%
AATATTGAAGAAGTTCCAAC+TGG + chr4_1:25661300-25661319 Msa0541670:CDS 30.0%
ACAATGAGTTATGAACTTTG+AGG - chr4_1:25661577-25661596 None:intergenic 30.0%
AAACCGATACTTCAAATTGA+AGG - chr4_1:25661915-25661934 None:intergenic 30.0%
!!! GGTTTTAAATAGGTTTACGT+AGG + chr4_1:25661930-25661949 Msa0541670:intron 30.0%
GAAACCAAATCAATCAAATG+TGG - chr4_1:25661968-25661987 None:intergenic 30.0%
GATTATTTCATCCAACACAA+GGG - chr4_1:25662015-25662034 None:intergenic 30.0%
TGATTATTTCATCCAACACA+AGG - chr4_1:25662016-25662035 None:intergenic 30.0%
! TACCTCTACTTGATTTTGTA+AGG - chr4_1:25662051-25662070 None:intergenic 30.0%
GTTTATAATGTTAGGCTTGT+TGG - chr4_1:25662122-25662141 None:intergenic 30.0%
CCTTGAAGAAAAATTGAAGT+TGG - chr4_1:25662499-25662518 None:intergenic 30.0%
! CCAACTTCAATTTTTCTTCA+AGG + chr4_1:25662496-25662515 Msa0541670:CDS 30.0%
!! AATGTAGAGACTTGTTGATA+AGG - chr4_1:25662785-25662804 None:intergenic 30.0%
ATCAACAAGTCTCTACATTA+TGG + chr4_1:25662786-25662805 Msa0541670:three_prime_UTR 30.0%
ATGAATCTTGATATTCAAGG+AGG - chr4_1:25662863-25662882 None:intergenic 30.0%
!! CGTCTGAATTTGTTATCAAA+TGG + chr4_1:25662888-25662907 Msa0541670:three_prime_UTR 30.0%
!!! TGTTGAGTTGGTATTTTTAG+TGG - chr4_1:25662947-25662966 None:intergenic 30.0%
AATTAGATTTGTCCTTGAGT+TGG - chr4_1:25662990-25663009 None:intergenic 30.0%
CAAAAGCTTCTTAATCCATA+TGG + chr4_1:25663214-25663233 Msa0541670:three_prime_UTR 30.0%
CTTCTTAATCCATATGGATT+CGG + chr4_1:25663220-25663239 Msa0541670:three_prime_UTR 30.0%
AACATGTTTGTAGACTTGTA+GGG - chr4_1:25663290-25663309 None:intergenic 30.0%
!! CATATGTCACTTTAATTTGG+TGG - chr4_1:25663565-25663584 None:intergenic 30.0%
! TGTAGCAAAATTAATGGATG+AGG + chr4_1:25663601-25663620 Msa0541670:three_prime_UTR 30.0%
AATCCTAGAGATATCATATC+AGG + chr4_1:25663758-25663777 Msa0541670:three_prime_UTR 30.0%
! TGAGACAGTTAATTTTCTCA+CGG + chr4_1:25663869-25663888 Msa0541670:three_prime_UTR 30.0%
GGACTCTTGAAAATGATAGA+AGG - chr4_1:25660764-25660783 None:intergenic 35.0%
ATGAGCTATCAAGATATGAG+AGG - chr4_1:25660785-25660804 None:intergenic 35.0%
GTATGTAGAGTCAACAAGAA+AGG - chr4_1:25660839-25660858 None:intergenic 35.0%
!! GTAGTGGACAATCTTGAAAA+GGG + chr4_1:25661000-25661019 Msa0541670:CDS 35.0%
!! TATGAACTTTGAGGCAATTG+AGG - chr4_1:25661568-25661587 None:intergenic 35.0%
ATCAATGTTGTATCAGTGTC+AGG + chr4_1:25661861-25661880 Msa0541670:intron 35.0%
! ACGCCTTCAATTTGAAGTAT+CGG + chr4_1:25661909-25661928 Msa0541670:intron 35.0%
ACAGCCACATTTGATTGATT+TGG + chr4_1:25661961-25661980 Msa0541670:intron 35.0%
GTCCTTACAAAATCAAGTAG+AGG + chr4_1:25662046-25662065 Msa0541670:CDS 35.0%
CATAGTCATTATTAGCAGGA+TGG - chr4_1:25662465-25662484 None:intergenic 35.0%
!! ATTAAAGGGAAGTACCGTAT+TGG - chr4_1:25662685-25662704 None:intergenic 35.0%
! TATCATTTTCAGCACACTGA+TGG + chr4_1:25663163-25663182 Msa0541670:three_prime_UTR 35.0%
! GATTAAGAAGCTTTTGTCGT+TGG - chr4_1:25663210-25663229 None:intergenic 35.0%
CAACATGTTTGTAGACTTGT+AGG - chr4_1:25663291-25663310 None:intergenic 35.0%
TAGCAGAATGAGTGAAAAGA+AGG + chr4_1:25663454-25663473 Msa0541670:three_prime_UTR 35.0%
AGCAGAATGAGTGAAAAGAA+GGG + chr4_1:25663455-25663474 Msa0541670:three_prime_UTR 35.0%
ATGATCGATGGTTTGAATGA+AGG + chr4_1:25663507-25663526 Msa0541670:three_prime_UTR 35.0%
TGATCGATGGTTTGAATGAA+GGG + chr4_1:25663508-25663527 Msa0541670:three_prime_UTR 35.0%
!! TCACTTTAATTTGGTGGACT+AGG - chr4_1:25663559-25663578 None:intergenic 35.0%
! CAAGATTGATTCTTGTGTTG+TGG + chr4_1:25663825-25663844 Msa0541670:three_prime_UTR 35.0%
TCTGCATGGGAATGAGTTAA+TGG + chr4_1:25660565-25660584 Msa0541670:five_prime_UTR 40.0%
TGATACTCAAAAGCAGAAGC+AGG - chr4_1:25660701-25660720 None:intergenic 40.0%
!! CAAACACTGGTTTCTGTTTC+TGG - chr4_1:25660736-25660755 None:intergenic 40.0%
ATAGAAGGAAAGGCAAACAC+TGG - chr4_1:25660749-25660768 None:intergenic 40.0%
CAAGAAAGGGTTGTTGGTAT+TGG - chr4_1:25660825-25660844 None:intergenic 40.0%
AGTCAACAAGAAAGGGTTGT+TGG - chr4_1:25660831-25660850 None:intergenic 40.0%
TTCAAGATTGTCCACTACCA+TGG - chr4_1:25660998-25661017 None:intergenic 40.0%
!! GGTAGTGGACAATCTTGAAA+AGG + chr4_1:25660999-25661018 Msa0541670:CDS 40.0%
ACAAAAGAAGAGTAGTGGTG+AGG + chr4_1:25661227-25661246 Msa0541670:CDS 40.0%
CAAAAGAAGAGTAGTGGTGA+GGG + chr4_1:25661228-25661247 Msa0541670:CDS 40.0%
CTGACATGGATATAACCAGT+TGG - chr4_1:25661318-25661337 None:intergenic 40.0%
ATATCCATGTCAGAGCAAGA+AGG + chr4_1:25661325-25661344 Msa0541670:CDS 40.0%
TATCCATGTCAGAGCAAGAA+GGG + chr4_1:25661326-25661345 Msa0541670:CDS 40.0%
TATTGCATACCCTTTCAGCA+AGG - chr4_1:25661374-25661393 None:intergenic 40.0%
! TACTACACTGATGGAAGTGT+CGG - chr4_1:25661447-25661466 None:intergenic 40.0%
! GTCTTGTCAGACAAAAGTGT+CGG + chr4_1:25661502-25661521 Msa0541670:intron 40.0%
TATTTCCTCACCTGATGATC+TGG - chr4_1:25662242-25662261 None:intergenic 40.0%
! CTCTGTCACTGAATTTTCAG+AGG + chr4_1:25662341-25662360 Msa0541670:intron 40.0%
!! ACATGTGGTATTGGTGTTGA+TGG - chr4_1:25662385-25662404 None:intergenic 40.0%
CACAATGGTACATGTGGTAT+TGG - chr4_1:25662394-25662413 None:intergenic 40.0%
! CAATTTTTCTTCAAGGGCAG+AGG + chr4_1:25662503-25662522 Msa0541670:CDS 40.0%
GAGGTCAAATACCTTCTTTG+AGG + chr4_1:25662522-25662541 Msa0541670:CDS 40.0%
! GATCTAAGGTACCTCAAAGA+AGG - chr4_1:25662536-25662555 None:intergenic 40.0%
TTGGCTCATGAGATAATGTC+AGG + chr4_1:25662590-25662609 Msa0541670:three_prime_UTR 40.0%
TGGCTCATGAGATAATGTCA+GGG + chr4_1:25662591-25662610 Msa0541670:three_prime_UTR 40.0%
GGACTATAACGATGTTGAGT+TGG - chr4_1:25662959-25662978 None:intergenic 40.0%
!! GTCCTTGAGTTGGTATTAGA+GGG - chr4_1:25662980-25662999 None:intergenic 40.0%
!! TGTCCTTGAGTTGGTATTAG+AGG - chr4_1:25662981-25663000 None:intergenic 40.0%
! CACTCATTCTGCTACTTGTT+TGG - chr4_1:25663448-25663467 None:intergenic 40.0%
AAGGGATTCTGTTCTATGCT+TGG + chr4_1:25663526-25663545 Msa0541670:three_prime_UTR 40.0%
ATTCTGTTCTATGCTTGGCA+TGG + chr4_1:25663531-25663550 Msa0541670:three_prime_UTR 40.0%
! TAATGGATGAGGAAGCCAAA+AGG + chr4_1:25663612-25663631 Msa0541670:three_prime_UTR 40.0%
CCTCCTGATATGATATCTCT+AGG - chr4_1:25663764-25663783 None:intergenic 40.0%
CCTAGAGATATCATATCAGG+AGG + chr4_1:25663761-25663780 Msa0541670:three_prime_UTR 40.0%
AGAGATATCATATCAGGAGG+AGG + chr4_1:25663764-25663783 Msa0541670:three_prime_UTR 40.0%
! GATTGATTCTTGTGTTGTGG+TGG + chr4_1:25663828-25663847 Msa0541670:three_prime_UTR 40.0%
GGTCTGTCACATAAACTCAG+AGG - chr4_1:25660649-25660668 None:intergenic 45.0%
TTGGACTCAGTCGCAAAAGT+TGG - chr4_1:25660670-25660689 None:intergenic 45.0%
!! CTGGTTTCTGTTTCTGGTGT+TGG - chr4_1:25660730-25660749 None:intergenic 45.0%
TGCTGAATCATCCATGGTAG+TGG + chr4_1:25660984-25661003 Msa0541670:CDS 45.0%
TTTGAGTAGCCCCTTGTCTA+AGG + chr4_1:25661086-25661105 Msa0541670:CDS 45.0%
AGTAGTGGTGAGGGAATGAA+AGG + chr4_1:25661237-25661256 Msa0541670:CDS 45.0%
ATCCATGTCAGAGCAAGAAG+GGG + chr4_1:25661327-25661346 Msa0541670:CDS 45.0%
TGTCTGTGTTAGGGCTTCAT+AGG - chr4_1:25661406-25661425 None:intergenic 45.0%
CGGTGTTTGTGTCTGTGTTA+GGG - chr4_1:25661415-25661434 None:intergenic 45.0%
! TCGGTGTTTGTGTCTGTGTT+AGG - chr4_1:25661416-25661435 None:intergenic 45.0%
!! CAAAAGTGTCGGAGCTACAT+AGG + chr4_1:25661513-25661532 Msa0541670:intron 45.0%
ATATTGCAGCAACTTGTGCC+TGG + chr4_1:25661661-25661680 Msa0541670:CDS 45.0%
CACTAACACCTATCCGGTAT+CGG + chr4_1:25661884-25661903 Msa0541670:intron 45.0%
AATGGACCTAGACACACTCT+TGG + chr4_1:25662211-25662230 Msa0541670:CDS 45.0%
! GTCTAACCAAGAGTGTGTCT+AGG - chr4_1:25662220-25662239 None:intergenic 45.0%
CTTGGTTAGACCAGATCATC+AGG + chr4_1:25662229-25662248 Msa0541670:CDS 45.0%
TTAGACCAGATCATCAGGTG+AGG + chr4_1:25662234-25662253 Msa0541670:intron 45.0%
AACTGTGTCAGCAAAAGGTG+TGG - chr4_1:25662431-25662450 None:intergenic 45.0%
GTGGTAACTGTGTCAGCAAA+AGG - chr4_1:25662436-25662455 None:intergenic 45.0%
TTTGCTGACACAGTTACCAC+AGG + chr4_1:25662436-25662455 Msa0541670:CDS 45.0%
GGCTCATGAGATAATGTCAG+GGG + chr4_1:25662592-25662611 Msa0541670:three_prime_UTR 45.0%
GTCCCTCTAATACCAACTCA+AGG + chr4_1:25662975-25662994 Msa0541670:three_prime_UTR 45.0%
GGACTATAACGATGCTGAGT+TGG + chr4_1:25663030-25663049 Msa0541670:three_prime_UTR 45.0%
!! AGCACACTGATGGACCATTT+TGG + chr4_1:25663173-25663192 Msa0541670:three_prime_UTR 45.0%
!! GCACACTGATGGACCATTTT+GGG + chr4_1:25663174-25663193 Msa0541670:three_prime_UTR 45.0%
TGGTCTTCTACGTCCCAAAA+TGG - chr4_1:25663190-25663209 None:intergenic 45.0%
! GTGTTGTTGCCGAATCCATA+TGG - chr4_1:25663232-25663251 None:intergenic 45.0%
! GTCGGCATTTTCTGTCCTTT+TGG - chr4_1:25663630-25663649 None:intergenic 45.0%
TGATTCTTGTGTTGTGGTGG+TGG + chr4_1:25663831-25663850 Msa0541670:three_prime_UTR 45.0%
!! TATATGATTATAAATATAAT+TGG + chr4_1:25662571-25662590 Msa0541670:three_prime_UTR 5.0%
GCAGAAGCAGGTATCAGTGT+TGG - chr4_1:25660689-25660708 None:intergenic 50.0%
CCATGGATGATTCAGCACTC+TGG - chr4_1:25660981-25661000 None:intergenic 50.0%
!! CCAGAGTGCTGAATCATCCA+TGG + chr4_1:25660978-25660997 Msa0541670:CDS 50.0%
TGGTGAGGGAATGAAAGGAG+AGG + chr4_1:25661242-25661261 Msa0541670:CDS 50.0%
ATAGCCACAGCCTTGCTGAA+AGG + chr4_1:25661361-25661380 Msa0541670:CDS 50.0%
TAGCCACAGCCTTGCTGAAA+GGG + chr4_1:25661362-25661381 Msa0541670:CDS 50.0%
ATACCCTTTCAGCAAGGCTG+TGG - chr4_1:25661368-25661387 None:intergenic 50.0%
!! GGAAGTGTCGGTGTTGTGTT+CGG - chr4_1:25661435-25661454 None:intergenic 50.0%
AACTTGTGCCTGGCTGTGAT+AGG + chr4_1:25661671-25661690 Msa0541670:CDS 50.0%
ACTTGTGCCTGGCTGTGATA+GGG + chr4_1:25661672-25661691 Msa0541670:CDS 50.0%
GTCAGGCACTAACACCTATC+CGG + chr4_1:25661878-25661897 Msa0541670:intron 50.0%
! ATTGAAGGCGTGACCGATAC+CGG - chr4_1:25661900-25661919 None:intergenic 50.0%
AACTGGAAAGGCCCTTGTGT+TGG + chr4_1:25662001-25662020 Msa0541670:CDS 50.0%
GAGCTGCACAATGGTACATG+TGG - chr4_1:25662400-25662419 None:intergenic 50.0%
! AGCAAAAGGTGTGGCATGGT+TGG - chr4_1:25662422-25662441 None:intergenic 50.0%
! TGTCAGCAAAAGGTGTGGCA+TGG - chr4_1:25662426-25662445 None:intergenic 50.0%
ATTAGCAGGATGGAAGCCTG+TGG - chr4_1:25662455-25662474 None:intergenic 50.0%
GACCCCTTCTTGCTCTGACA+TGG - chr4_1:25661332-25661351 None:intergenic 55.0%
TGTCAGAGCAAGAAGGGGTC+AGG + chr4_1:25661332-25661351 Msa0541670:CDS 55.0%
AGACTCACCCTATCACAGCC+AGG - chr4_1:25661682-25661701 None:intergenic 55.0%
GCATGGTTGGAGCTGCACAA+TGG - chr4_1:25662409-25662428 None:intergenic 55.0%
AGGCGTGACCGATACCGGAT+AGG - chr4_1:25661895-25661914 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 25660553 25663953 25660553 ID=Msa0541670;Name=Msa0541670
chr4_1 mRNA 25660553 25663953 25660553 ID=Msa0541670-mRNA-1;Parent=Msa0541670;Name=Msa0541670-mRNA-1;_AED=0.07;_eAED=0.07;_QI=252|1|1|1|0.71|0.62|8|1274|301
chr4_1 exon 25660553 25661107 25660553 ID=Msa0541670-mRNA-1:exon:6900;Parent=Msa0541670-mRNA-1
chr4_1 exon 25661198 25661383 25661198 ID=Msa0541670-mRNA-1:exon:6901;Parent=Msa0541670-mRNA-1
chr4_1 exon 25661628 25661693 25661628 ID=Msa0541670-mRNA-1:exon:6902;Parent=Msa0541670-mRNA-1
chr4_1 exon 25661999 25662067 25661999 ID=Msa0541670-mRNA-1:exon:6903;Parent=Msa0541670-mRNA-1
chr4_1 exon 25662161 25662250 25662161 ID=Msa0541670-mRNA-1:exon:6904;Parent=Msa0541670-mRNA-1
chr4_1 exon 25662361 25662733 25662361 ID=Msa0541670-mRNA-1:exon:6905;Parent=Msa0541670-mRNA-1
chr4_1 exon 25662760 25663309 25662760 ID=Msa0541670-mRNA-1:exon:6906;Parent=Msa0541670-mRNA-1
chr4_1 exon 25663411 25663953 25663411 ID=Msa0541670-mRNA-1:exon:6907;Parent=Msa0541670-mRNA-1
chr4_1 five_prime_UTR 25660553 25660804 25660553 ID=Msa0541670-mRNA-1:five_prime_utr;Parent=Msa0541670-mRNA-1
chr4_1 CDS 25660805 25661107 25660805 ID=Msa0541670-mRNA-1:cds;Parent=Msa0541670-mRNA-1
chr4_1 CDS 25661198 25661383 25661198 ID=Msa0541670-mRNA-1:cds;Parent=Msa0541670-mRNA-1
chr4_1 CDS 25661628 25661693 25661628 ID=Msa0541670-mRNA-1:cds;Parent=Msa0541670-mRNA-1
chr4_1 CDS 25661999 25662067 25661999 ID=Msa0541670-mRNA-1:cds;Parent=Msa0541670-mRNA-1
chr4_1 CDS 25662161 25662250 25662161 ID=Msa0541670-mRNA-1:cds;Parent=Msa0541670-mRNA-1
chr4_1 CDS 25662361 25662552 25662361 ID=Msa0541670-mRNA-1:cds;Parent=Msa0541670-mRNA-1
chr4_1 three_prime_UTR 25662553 25662733 25662553 ID=Msa0541670-mRNA-1:three_prime_utr;Parent=Msa0541670-mRNA-1
chr4_1 three_prime_UTR 25662760 25663309 25662760 ID=Msa0541670-mRNA-1:three_prime_utr;Parent=Msa0541670-mRNA-1
chr4_1 three_prime_UTR 25663411 25663953 25663411 ID=Msa0541670-mRNA-1:three_prime_utr;Parent=Msa0541670-mRNA-1
Gene Sequence

>Msa0541670

ATGGCAGCTTTTTCATACCAATACCAACAACCCTTTCTTGTTGACTCTACATACTTCACCAATATAAACACTTCTCTTCCTTCTCATCTTTTTCATAATCATCAAGAACTCTCTCTCAGTAATCAAGAAACTAGTTGTGTTGATCAAAACTCTAAGAATAGCATAAGTAACAACCAGAGTGCTGAATCATCCATGGTAGTGGACAATCTTGAAAAGGGTGAGCAAGTTACTCAGAAAGTTACTTCTAAGGAGAAGAAAAGAAGAATCAGAAATAAGACTTCTTTGAGTAGCCCCTTGTCTAAGGATATCTCAAAAGAAGGGAAAAGCAAAAAACAAAAGAAGAGTAGTGGTGAGGGAATGAAAGGAGAGGAGAAGAAAAAAGAAGAGAAGAAGGATCAAAAGAAAAATATTGAAGAAGTTCCAACTGGTTATATCCATGTCAGAGCAAGAAGGGGTCAGGCAACAGATAGCCACAGCCTTGCTGAAAGGGTGAGAAGAGAAAAAATAAGTGAAAGAATGAAGATATTGCAGCAACTTGTGCCTGGCTGTGATAGGGTAACTGGAAAGGCCCTTGTGTTGGATGAAATAATCAATTATGTACAGTCCTTACAAAATCAAGTAGAGTTCTTGTCAATGAAACTTGCTTCAGTGAATCCAATCTTCTTTGACTTTACAATGGACCTAGACACACTCTTGGTTAGACCAGATCATCAGAGGTTAAATAGCATAACATCACCATCAACACCAATACCACATGTACCATTGTGCAGCTCCAACCATGCCACACCTTTTGCTGACACAGTTACCACAGGCTTCCATCCTGCTAATAATGACTATGTTTTGGATTATCCAACTTCAATTTTTCTTCAAGGGCAGAGGTCAAATACCTTCTTTGAGGTACCTTAG
Protein sequence

>Msa0541670

MAAFSYQYQQPFLVDSTYFTNINTSLPSHLFHNHQELSLSNQETSCVDQNSKNSISNNQSAESSMVVDNLEKGEQVTQKVTSKEKKRRIRNKTSLSSPLSKDISKEGKSKKQKKSSGEGMKGEEKKKEEKKDQKKNIEEVPTGYIHVRARRGQATDSHSLAERVRREKISERMKILQQLVPGCDRVTGKALVLDEIINYVQSLQNQVEFLSMKLASVNPIFFDFTMDLDTLLVRPDHQRLNSITSPSTPIPHVPLCSSNHATPFADTVTTGFHPANNDYVLDYPTSIFLQGQRSNTFFEVP*