AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0542730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0542730 MtrunA17_Chr4g0022491 99.163 239 2 0 1 239 1 239 8.68e-179 490
Msa0542730 MtrunA17_Chr2g0323881 77.119 236 54 0 4 239 1 236 5.43e-139 389
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0542730 AT4G28190.1 74.890 227 57 0 13 239 11 237 5.77e-129 364
Msa0542730 AT4G28190.2 65.891 258 57 1 13 239 11 268 4.27e-122 348
Msa0542730 AT2G20825.1 69.643 224 62 1 16 239 10 227 8.57e-118 335

Find 67 sgRNAs with CRISPR-Local

Find 275 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGACCAACCCGGCCATTT+TGG 0.182520 4_1:+28073156 None:intergenic
GAAATGCTTAATTTCCTAAT+TGG 0.196599 4_1:-28071075 Msa0542730:three_prime_UTR
AATATCTGGGTTATAATTAA+TGG 0.255756 4_1:-28073260 Msa0542730:CDS
GCACTTCTTGTGTGTGCTTC+GGG 0.294039 4_1:-28071252 Msa0542730:CDS
TGCACTTCTTGTGTGTGCTT+CGG 0.308806 4_1:-28071253 Msa0542730:CDS
GGAAATGGAAGAATAATATC+TGG 0.324881 4_1:-28073274 Msa0542730:CDS
CTTTCACTGTTCTCATTGAC+TGG 0.327911 4_1:-28071031 Msa0542730:three_prime_UTR
CAGACTGTTGATGTCCAATT+AGG 0.333052 4_1:+28071061 None:intergenic
GGCATAAATAACCAGCTTAT+AGG 0.337643 4_1:+28071112 None:intergenic
AATGGGTTGGAGAAGGGAAA+TGG 0.345803 4_1:-28075225 Msa0542730:CDS
GCATTTCTAAAATTCTATAT+AGG 0.363394 4_1:+28071091 None:intergenic
CAGCTTATAGGAACAAGTAT+TGG 0.380586 4_1:+28071124 None:intergenic
ATGAGTTTGTTCGCTGTACT+AGG 0.392965 4_1:-28073130 Msa0542730:CDS
ATGCTGTGTCAGATGCAAAT+TGG 0.397171 4_1:-28073049 Msa0542730:CDS
GGCTCTCAGAGATCCCAAAA+TGG 0.420579 4_1:-28073170 Msa0542730:CDS
ATGGGTTGGAGAAGGGAAAT+GGG 0.422445 4_1:-28075224 Msa0542730:CDS
GGTTATAATTAATGGTGATA+AGG 0.431324 4_1:-28073252 Msa0542730:CDS
GAAGAGAGACAGCAAGGAAA+TGG 0.432232 4_1:-28073289 Msa0542730:CDS
GAATAAACGTACTTCTCATA+TGG 0.434948 4_1:+28073011 None:intergenic
TGAAGGGTATAGATATATAG+AGG 0.440539 4_1:+28075321 None:intergenic
CAATACTTGTTCCTATAAGC+TGG 0.440692 4_1:-28071123 Msa0542730:three_prime_UTR
CAAGTGGCGAAAGCTGCTAA+TGG 0.441867 4_1:-28073191 Msa0542730:CDS
TTTATGGAGAAAATGGAGAA+TGG 0.445958 4_1:-28075243 Msa0542730:CDS
TTATGGAGAAAATGGAGAAT+GGG 0.453953 4_1:-28075242 Msa0542730:CDS
AAGAGCTGAGAGAAATGAGT+GGG 0.458242 4_1:-28075182 Msa0542730:CDS
TAATGGTGATAAGGTTCCAT+TGG 0.460779 4_1:-28073243 Msa0542730:CDS
TGCACTCCTGGTTGTGATGA+AGG 0.473540 4_1:-28075042 Msa0542730:intron
ATGGAGAATGGGTTGGAGAA+GGG 0.474047 4_1:-28075231 Msa0542730:CDS
TGATGCTGTTGGTAGACTTA+GGG 0.474100 4_1:-28075100 Msa0542730:CDS
AACCTTTGCACGTTGGTGAT+CGG 0.474249 4_1:+28071275 None:intergenic
CTCAGAGATCCCAAAATGGC+CGG 0.484048 4_1:-28073166 Msa0542730:CDS
AGCTTATAGGAACAAGTATT+GGG 0.484598 4_1:+28071125 None:intergenic
AATGGTGATAAGGTTCCATT+GGG 0.489767 4_1:-28073242 Msa0542730:CDS
AATGGAGAATGGGTTGGAGA+AGG 0.494806 4_1:-28075232 Msa0542730:CDS
TGGTAAAGTCAGTACAAGTC+TGG 0.500731 4_1:+28071197 None:intergenic
GTGATGCTGTTGGTAGACTT+AGG 0.502004 4_1:-28075101 Msa0542730:CDS
ATTATTTGTGAATGCACTCC+TGG 0.510243 4_1:-28075054 Msa0542730:CDS
GGAGAAAATGGAGAATGGGT+TGG 0.523981 4_1:-28075238 Msa0542730:CDS
ATTTCGTCTGAGATCTAAAG+AGG 0.537276 4_1:-28073090 Msa0542730:CDS
GGCCGATCACCAACGTGCAA+AGG 0.545531 4_1:-28071277 Msa0542730:CDS
AGATCCCAAAATGGCCGGGT+TGG 0.546651 4_1:-28073161 Msa0542730:CDS
GAGTGTTTCTCAAATGCAGC+AGG 0.552189 4_1:+28073314 None:intergenic
AGTGTAAAATCAAAGTGTGA+AGG 0.557982 4_1:+28075304 None:intergenic
TCAGAGATCCCAAAATGGCC+GGG 0.567774 4_1:-28073165 Msa0542730:CDS
ACACAACGCTGTTGTTGTTG+AGG 0.570370 4_1:+28071149 None:intergenic
GAAATGGAAGAATAATATCT+GGG 0.580008 4_1:-28073273 Msa0542730:CDS
CGAAGAGTTTACAGAGGATG+CGG 0.588649 4_1:-28071298 Msa0542730:CDS
GTGTAAAATCAAAGTGTGAA+GGG 0.589215 4_1:+28075305 None:intergenic
GCTGCATTTGAGAAACACTC+TGG 0.595645 4_1:-28073311 Msa0542730:CDS
TTGCATCTGACACAGCATCA+TGG 0.597955 4_1:+28073054 None:intergenic
GGGTGTACTAGTCATAGATA+TGG 0.599115 4_1:-28075123 Msa0542730:CDS
AGGGTATAGATATATAGAGG+AGG 0.605441 4_1:+28075324 None:intergenic
ACTCATCGCGGTGACCAACC+CGG 0.606791 4_1:+28073147 None:intergenic
AGAGCTGAGAGAAATGAGTG+GGG 0.614369 4_1:-28075181 Msa0542730:CDS
CATAGATATGGTGATGCTGT+TGG 0.619627 4_1:-28075111 Msa0542730:CDS
GCTCAAATACTACAATCAAG+TGG 0.629351 4_1:-28073207 Msa0542730:CDS
ACACAGCATCATGGTGAATG+CGG 0.634639 4_1:+28073063 None:intergenic
TGGGTTGTCAATGTTTAGCG+AGG 0.646931 4_1:-28075205 Msa0542730:CDS
GAAGAGCTGAGAGAAATGAG+TGG 0.646981 4_1:-28075183 Msa0542730:CDS
GGAAGTCGAAGAGTTTACAG+AGG 0.668670 4_1:-28071304 Msa0542730:CDS
ACTCTGGAAGAGAGACAGCA+AGG 0.669740 4_1:-28073295 Msa0542730:CDS
GAAGTGCAACCTTTGCACGT+TGG 0.672088 4_1:+28071268 None:intergenic
GAATATGTTGAAGTCACGTG+TGG 0.677819 4_1:-28075144 Msa0542730:CDS
GAGGGACCTTCATCACAACC+AGG 0.689911 4_1:+28075036 None:intergenic
AATATGTTGAAGTCACGTGT+GGG 0.722833 4_1:-28075143 Msa0542730:CDS
TGTGATGATGAAGAAGAACG+AGG 0.758234 4_1:-28071325 Msa0542730:CDS
TACAGCGAACAAACTCATCG+CGG 0.760016 4_1:+28073135 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCAAATTAAATTATGA+AGG + chr4_1:28072549-28072568 None:intergenic 10.0%
!!! CTTCTTTTATTTATATTTTT+AGG + chr4_1:28072631-28072650 None:intergenic 10.0%
!! AAAACAAAATCATAAATTAT+TGG + chr4_1:28072816-28072835 None:intergenic 10.0%
!!! AATAATTTATGATTTTGTTT+TGG - chr4_1:28072815-28072834 Msa0542730:intron 10.0%
!! AAATAAAAATAAAATAAGTG+AGG - chr4_1:28073424-28073443 Msa0542730:intron 10.0%
!! TAATATATAATCTTTGAATT+TGG - chr4_1:28073773-28073792 Msa0542730:intron 10.0%
!! AATATATAATCTTTGAATTT+GGG - chr4_1:28073774-28073793 Msa0542730:intron 10.0%
!! TTATAGATATTTATATCATT+TGG - chr4_1:28074740-28074759 Msa0542730:intron 10.0%
!! AAATGTAGAAGATTTATAAT+TGG - chr4_1:28071728-28071747 Msa0542730:intron 15.0%
!!! TTTTATTGGTAATTTGTTAT+TGG - chr4_1:28074268-28074287 Msa0542730:intron 15.0%
!! ATTGAAATATCAAATGATAA+TGG + chr4_1:28074621-28074640 None:intergenic 15.0%
!!! TTTATTTTTGACCAAATTTA+TGG - chr4_1:28074708-28074727 Msa0542730:intron 15.0%
!!! TCTAAATATATGTCATTTTT+GGG + chr4_1:28074985-28075004 None:intergenic 15.0%
!!! TTCTAAATATATGTCATTTT+TGG + chr4_1:28074986-28075005 None:intergenic 15.0%
!! TTGTGAGATTAAATTCAATA+TGG - chr4_1:28071763-28071782 Msa0542730:intron 20.0%
!! GATTAGTAATTAATATCCTA+AGG - chr4_1:28071917-28071936 Msa0542730:intron 20.0%
!! TTATAGATTAACAATACCTT+AGG + chr4_1:28071936-28071955 None:intergenic 20.0%
!! TACTTGAACATTGATATATA+CGG - chr4_1:28072307-28072326 Msa0542730:intron 20.0%
!! CAAATTAAATTATGAAGGTA+TGG + chr4_1:28072544-28072563 None:intergenic 20.0%
!! AATTTGAAAAATCAACAACT+TGG - chr4_1:28072558-28072577 Msa0542730:intron 20.0%
!! ATTTGAAAAATCAACAACTT+GGG - chr4_1:28072559-28072578 Msa0542730:intron 20.0%
!! TCAATAATTAGTCATATAGA+CGG + chr4_1:28072716-28072735 None:intergenic 20.0%
!! ATATGACTAATTATTGAATG+TGG - chr4_1:28072719-28072738 Msa0542730:intron 20.0%
!! ACATGGAAAAAAATATACAA+TGG + chr4_1:28072885-28072904 None:intergenic 20.0%
!! TTACATATAAGTAGTGTTAA+TGG - chr4_1:28072961-28072980 Msa0542730:intron 20.0%
!! AACATCATATGCAAAAATTA+AGG + chr4_1:28073008-28073027 None:intergenic 20.0%
!! AATATCTGGGTTATAATTAA+TGG - chr4_1:28073104-28073123 Msa0542730:CDS 20.0%
!! AAAAGAAAAGACCAAATTTA+TGG + chr4_1:28073932-28073951 None:intergenic 20.0%
!!! ATAATTGATAGTCTTAATCT+AGG - chr4_1:28074021-28074040 Msa0542730:intron 20.0%
!!! CATATCATATTACTGTTTTA+CGG - chr4_1:28074116-28074135 Msa0542730:intron 20.0%
!!! AATCTTTTGCATAAAATTTC+AGG - chr4_1:28074234-28074253 Msa0542730:intron 20.0%
!!! ATCTTTTGCATAAAATTTCA+GGG - chr4_1:28074235-28074254 Msa0542730:intron 20.0%
!!! AGGGATGAAATATATTTTAT+TGG - chr4_1:28074254-28074273 Msa0542730:intron 20.0%
!! AAAACTAATAGCCATAAATT+TGG + chr4_1:28074722-28074741 None:intergenic 20.0%
!!! TTTTATAATCAACCATAAGT+TGG + chr4_1:28074812-28074831 None:intergenic 20.0%
!! ATTATAAAAGACTCAGAATT+TGG - chr4_1:28074823-28074842 Msa0542730:intron 20.0%
!! TTATAAAAGACTCAGAATTT+GGG - chr4_1:28074824-28074843 Msa0542730:intron 20.0%
!!! CTAAATATATGTCATTTTTG+GGG + chr4_1:28074984-28075003 None:intergenic 20.0%
!!! TTAGTAATAGTAAGTTTTTC+AGG - chr4_1:28075010-28075029 Msa0542730:intron 20.0%
!!! GCATTTCTAAAATTCTATAT+AGG + chr4_1:28075276-28075295 None:intergenic 20.0%
! TTGACCCAAAAAAACTATAT+GGG + chr4_1:28071088-28071107 None:intergenic 25.0%
! TTTGACCCAAAAAAACTATA+TGG + chr4_1:28071089-28071108 None:intergenic 25.0%
!!! CAAAAACAGAGTCTTTTTTA+TGG - chr4_1:28071105-28071124 Msa0542730:three_prime_UTR 25.0%
!!! AGTCTTTTTTATGGAGAAAA+TGG - chr4_1:28071114-28071133 Msa0542730:three_prime_UTR 25.0%
! GTATAAGTATTTATGCATGT+CGG + chr4_1:28071453-28071472 None:intergenic 25.0%
!!! TACATTTTTTTTACCTGAGT+CGG - chr4_1:28071515-28071534 Msa0542730:intron 25.0%
! ATAAAATACTAACACACACA+CGG + chr4_1:28071552-28071571 None:intergenic 25.0%
!! TGTGTGTGTTAGTATTTTAT+AGG - chr4_1:28071552-28071571 Msa0542730:intron 25.0%
!!! TTATAGGTTGATAGTGTATA+TGG - chr4_1:28071568-28071587 Msa0542730:intron 25.0%
!! ATGTATGTTCTTTTCTTTGA+TGG - chr4_1:28071969-28071988 Msa0542730:intron 25.0%
! AATTAAGTGGAATGAATATG+AGG + chr4_1:28072047-28072066 None:intergenic 25.0%
! TAATAAACTAACACACATAG+TGG + chr4_1:28072289-28072308 None:intergenic 25.0%
! ACTTGAACATTGATATATAC+GGG - chr4_1:28072308-28072327 Msa0542730:intron 25.0%
! TGAGAATTTGTGATTTAAAC+TGG - chr4_1:28072386-28072405 Msa0542730:intron 25.0%
! TCATTCATATATCCAATGAT+AGG - chr4_1:28072844-28072863 Msa0542730:intron 25.0%
! GAAATGGAAGAATAATATCT+GGG - chr4_1:28073091-28073110 Msa0542730:CDS 25.0%
! GGTTATAATTAATGGTGATA+AGG - chr4_1:28073112-28073131 Msa0542730:CDS 25.0%
! TGAAAATGAGTTTATAAGTG+GGG + chr4_1:28073628-28073647 None:intergenic 25.0%
! GTGAAAATGAGTTTATAAGT+GGG + chr4_1:28073629-28073648 None:intergenic 25.0%
! TATAATCTTTGAATTTGGGT+TGG - chr4_1:28073778-28073797 Msa0542730:intron 25.0%
!! ATAATCTTTGAATTTGGGTT+GGG - chr4_1:28073779-28073798 Msa0542730:intron 25.0%
! CTGAAAATGAGTTTATAAGT+GGG + chr4_1:28073838-28073857 None:intergenic 25.0%
! TCTGAAAATGAGTTTATAAG+TGG + chr4_1:28073839-28073858 None:intergenic 25.0%
! AAAAGACCAAATTTATGGAT+TGG + chr4_1:28073927-28073946 None:intergenic 25.0%
! AGCAGTTATTTATCATAGTT+CGG + chr4_1:28074213-28074232 None:intergenic 25.0%
! TTCTTAAAGTTACTAAGTCT+TGG - chr4_1:28074305-28074324 Msa0542730:intron 25.0%
! ATACAGTTTATGTTCCATAT+TGG - chr4_1:28074333-28074352 Msa0542730:intron 25.0%
!! AATATTGTGTTTTCATCCAA+CGG + chr4_1:28074587-28074606 None:intergenic 25.0%
!!! GAAATATCAAATGATAATGG+TGG + chr4_1:28074618-28074637 None:intergenic 25.0%
! TAGTATAAGTGCAATTATGT+AGG + chr4_1:28074888-28074907 None:intergenic 25.0%
!!! CATTTTTGGGGTAATAATTA+AGG + chr4_1:28074972-28074991 None:intergenic 25.0%
! GAAATGCTTAATTTCCTAAT+TGG - chr4_1:28075289-28075308 Msa0542730:five_prime_UTR 25.0%
! TGAAGGGTATAGATATATAG+AGG + chr4_1:28071046-28071065 None:intergenic 30.0%
GTGTAAAATCAAAGTGTGAA+GGG + chr4_1:28071062-28071081 None:intergenic 30.0%
AGTGTAAAATCAAAGTGTGA+AGG + chr4_1:28071063-28071082 None:intergenic 30.0%
!!! ACTGACCCATATAGTTTTTT+TGG - chr4_1:28071080-28071099 Msa0542730:three_prime_UTR 30.0%
!!! CTGACCCATATAGTTTTTTT+GGG - chr4_1:28071081-28071100 Msa0542730:three_prime_UTR 30.0%
TTTATGGAGAAAATGGAGAA+TGG - chr4_1:28071121-28071140 Msa0542730:three_prime_UTR 30.0%
TTATGGAGAAAATGGAGAAT+GGG - chr4_1:28071122-28071141 Msa0542730:three_prime_UTR 30.0%
AACATAATGTAACCGTAGTA+AGG - chr4_1:28071846-28071865 Msa0542730:intron 30.0%
! TGACTTTGAAGAAATATCCT+AGG + chr4_1:28071873-28071892 None:intergenic 30.0%
! GAACATACATAACTTGTACT+AGG + chr4_1:28071959-28071978 None:intergenic 30.0%
CATAGTGACTGAAAATTAAG+TGG + chr4_1:28072060-28072079 None:intergenic 30.0%
AAACATGACATTACGACATA+TGG + chr4_1:28072218-28072237 None:intergenic 30.0%
GTCGTAATGTCATGTTTATA+TGG - chr4_1:28072221-28072240 Msa0542730:intron 30.0%
GTAATGTCATGTTTATATGG+CGG - chr4_1:28072224-28072243 Msa0542730:intron 30.0%
! TGGATTAGAGTTATCAATGA+TGG + chr4_1:28072269-28072288 None:intergenic 30.0%
! TTAAATTATGAAGGTATGGC+AGG + chr4_1:28072540-28072559 None:intergenic 30.0%
TGACTAATTATTGAATGTGG+TGG - chr4_1:28072722-28072741 Msa0542730:intron 30.0%
!! TTGAATGTGGTGGATAATTT+AGG - chr4_1:28072732-28072751 Msa0542730:intron 30.0%
ATGAAAGTGAAACTAACAAG+TGG + chr4_1:28072766-28072785 None:intergenic 30.0%
ACTTATAAAGCTCCTATCAT+TGG + chr4_1:28072859-28072878 None:intergenic 30.0%
! TTGAGTTTTTCTTTTCACCA+TGG + chr4_1:28072919-28072938 None:intergenic 30.0%
GGAAATGGAAGAATAATATC+TGG - chr4_1:28073090-28073109 Msa0542730:CDS 30.0%
GAATAAACGTACTTCTCATA+TGG + chr4_1:28073356-28073375 None:intergenic 30.0%
AAGTACATAAAGATGCTCTT+TGG + chr4_1:28073524-28073543 None:intergenic 30.0%
CGTGAAAATGAGTTTATAAG+TGG + chr4_1:28073630-28073649 None:intergenic 30.0%
AAAACTATCTTGTAAGGTGA+GGG - chr4_1:28073806-28073825 Msa0542730:intron 30.0%
AAAATGAGTTTATAAGTGGG+AGG + chr4_1:28073626-28073645 None:intergenic 30.0%
TTAACAATGGAAATAGTGCA+CGG - chr4_1:28073884-28073903 Msa0542730:intron 30.0%
!! ATTTATGGATTGGCTTAACT+TGG + chr4_1:28073917-28073936 None:intergenic 30.0%
GTTAAGCCAATCCATAAATT+TGG - chr4_1:28073918-28073937 Msa0542730:intron 30.0%
CTATATCTTTAGATCCTACA+CGG + chr4_1:28073965-28073984 None:intergenic 30.0%
CTGGTTAAACTATTCCAATA+TGG + chr4_1:28074350-28074369 None:intergenic 30.0%
AAAATGTTCTTGAGCAAGAT+TGG + chr4_1:28074411-28074430 None:intergenic 30.0%
! AAATCCATTCAAGTGGTTTT+AGG + chr4_1:28074480-28074499 None:intergenic 30.0%
ATTAGGTAAATCCATTCAAG+TGG + chr4_1:28074487-28074506 None:intergenic 30.0%
TGGATTTACCTAATATCCTT+TGG - chr4_1:28074493-28074512 Msa0542730:intron 30.0%
!! CTTAAGTTCTAGAAATAAGC+TGG + chr4_1:28074564-28074583 None:intergenic 30.0%
!! TAACTGTTCTGCTTTTACAT+TGG - chr4_1:28074645-28074664 Msa0542730:intron 30.0%
AGTAGTTGAACAGACAATAA+AGG + chr4_1:28074777-28074796 None:intergenic 30.0%
AGCTTATAGGAACAAGTATT+GGG + chr4_1:28075242-28075261 None:intergenic 30.0%
AGGGTATAGATATATAGAGG+AGG + chr4_1:28071043-28071062 None:intergenic 35.0%
AATATGTTGAAGTCACGTGT+GGG - chr4_1:28071221-28071240 Msa0542730:CDS 35.0%
! AGACTTAGGGTTTTTCTTAG+TGG - chr4_1:28071277-28071296 Msa0542730:CDS 35.0%
ATTATTTGTGAATGCACTCC+TGG - chr4_1:28071310-28071329 Msa0542730:CDS 35.0%
!! GGTTGATAGTGTATATGGTT+TGG - chr4_1:28071573-28071592 Msa0542730:intron 35.0%
!! TAGTGTATATGGTTTGGTGA+AGG - chr4_1:28071579-28071598 Msa0542730:intron 35.0%
!! GGTTTGACTTTGATGAGTTT+TGG - chr4_1:28071600-28071619 Msa0542730:intron 35.0%
ATTTGTCTCAATGAACTCCT+TGG - chr4_1:28071661-28071680 Msa0542730:intron 35.0%
TCATTAGCATGATCAGAAAG+AGG + chr4_1:28071693-28071712 None:intergenic 35.0%
AATATCCTAGGACCTTACTA+CGG + chr4_1:28071861-28071880 None:intergenic 35.0%
GGACGATATAATAGATGTTG+CGG + chr4_1:28072185-28072204 None:intergenic 35.0%
ACGACATATGGCAGAAAAAT+TGG + chr4_1:28072206-28072225 None:intergenic 35.0%
TTAGAGTTATCAATGATGGC+TGG + chr4_1:28072265-28072284 None:intergenic 35.0%
TGATATATACGGGTAGAACT+TGG - chr4_1:28072318-28072337 Msa0542730:intron 35.0%
ACAGTATCTGTTGATGCAAA+AGG - chr4_1:28072342-28072361 Msa0542730:intron 35.0%
AAATTCTCATTTGTCAGCAG+AGG + chr4_1:28072375-28072394 None:intergenic 35.0%
TTTAAACTGGCAGTGTACTT+TGG - chr4_1:28072399-28072418 Msa0542730:intron 35.0%
!! AATTTTGTTTGCCACATTGC+AGG - chr4_1:28072423-28072442 Msa0542730:intron 35.0%
GGGAAGAGATCATATGATTA+AGG - chr4_1:28072579-28072598 Msa0542730:intron 35.0%
TAATGGTGATAAGGTTCCAT+TGG - chr4_1:28073121-28073140 Msa0542730:CDS 35.0%
AATGGTGATAAGGTTCCATT+GGG - chr4_1:28073122-28073141 Msa0542730:CDS 35.0%
GCTCAAATACTACAATCAAG+TGG - chr4_1:28073157-28073176 Msa0542730:CDS 35.0%
! ATTTCGTCTGAGATCTAAAG+AGG - chr4_1:28073274-28073293 Msa0542730:CDS 35.0%
AACAAAGTTCATTCGAAGCA+CGG + chr4_1:28073497-28073516 None:intergenic 35.0%
AATGAGTTTATAAGTGGGGA+AGG + chr4_1:28073624-28073643 None:intergenic 35.0%
!! AGGCATATCCATGATTTGTT+GGG + chr4_1:28073747-28073766 None:intergenic 35.0%
! TAGGCATATCCATGATTTGT+TGG + chr4_1:28073748-28073767 None:intergenic 35.0%
GGTTCCAAAACTATCTTGTA+AGG - chr4_1:28073800-28073819 Msa0542730:intron 35.0%
! CTCACCTTACAAGATAGTTT+TGG + chr4_1:28073807-28073826 None:intergenic 35.0%
CAAAACTATCTTGTAAGGTG+AGG - chr4_1:28073805-28073824 Msa0542730:intron 35.0%
GTCTCACATTGGATAATGTA+TGG + chr4_1:28073862-28073881 None:intergenic 35.0%
CATTGTTAAGAGTCTCACAT+TGG + chr4_1:28073873-28073892 None:intergenic 35.0%
CAATGTGAGACTCTTAACAA+TGG - chr4_1:28073871-28073890 Msa0542730:intron 35.0%
! TTTTCATTTTCACACCGTGT+AGG - chr4_1:28073948-28073967 Msa0542730:intron 35.0%
CTCTGCAAATTAGTAAGGAT+TGG + chr4_1:28073991-28074010 None:intergenic 35.0%
! TCATATTACTGTTTTACGGC+TGG - chr4_1:28074120-28074139 Msa0542730:intron 35.0%
! CATATTACTGTTTTACGGCT+GGG - chr4_1:28074121-28074140 Msa0542730:intron 35.0%
GCGATTATGTATCATTGATG+TGG - chr4_1:28074143-28074162 Msa0542730:intron 35.0%
! TCATTGATGTGGTCTCTTTT+CGG - chr4_1:28074154-28074173 Msa0542730:intron 35.0%
TTCTCTGTGTGTACTTTGTT+AGG - chr4_1:28074444-28074463 Msa0542730:intron 35.0%
!!! TCAAGTGGTTTTAGGATAGT+TGG + chr4_1:28074472-28074491 None:intergenic 35.0%
CTATCCTAAAACCACTTGAA+TGG - chr4_1:28074473-28074492 Msa0542730:intron 35.0%
CTTCAAGTCCAAAGGATATT+AGG + chr4_1:28074504-28074523 None:intergenic 35.0%
GATATATGCTTCAAGTCCAA+AGG + chr4_1:28074512-28074531 None:intergenic 35.0%
! AACGAGAGTACAATATGGAA+TGG + chr4_1:28074534-28074553 None:intergenic 35.0%
! TGAGAAACGAGAGTACAATA+TGG + chr4_1:28074539-28074558 None:intergenic 35.0%
!! ATTTCTAGAACTTAAGCCGT+TGG - chr4_1:28074568-28074587 Msa0542730:intron 35.0%
TTGTCTGTTCAACTACTGAA+TGG - chr4_1:28074780-28074799 Msa0542730:intron 35.0%
TGTCTGTTCAACTACTGAAT+GGG - chr4_1:28074781-28074800 Msa0542730:intron 35.0%
GGAAGATATCTAATTCCTTG+AGG + chr4_1:28074857-28074876 None:intergenic 35.0%
GCAATTATGTAGGTTATGTC+AGG + chr4_1:28074878-28074897 None:intergenic 35.0%
! GATTCAAGCTTTTACATTGC+TGG + chr4_1:28075190-28075209 None:intergenic 35.0%
CAGCTTATAGGAACAAGTAT+TGG + chr4_1:28075243-28075262 None:intergenic 35.0%
CAATACTTGTTCCTATAAGC+TGG - chr4_1:28075241-28075260 Msa0542730:CDS 35.0%
GGCATAAATAACCAGCTTAT+AGG + chr4_1:28075255-28075274 None:intergenic 35.0%
AAGAGCTGAGAGAAATGAGT+GGG - chr4_1:28071182-28071201 Msa0542730:CDS 40.0%
GAATATGTTGAAGTCACGTG+TGG - chr4_1:28071220-28071239 Msa0542730:CDS 40.0%
GGGTGTACTAGTCATAGATA+TGG - chr4_1:28071241-28071260 Msa0542730:CDS 40.0%
! CATAGATATGGTGATGCTGT+TGG - chr4_1:28071253-28071272 Msa0542730:CDS 40.0%
! TGATGCTGTTGGTAGACTTA+GGG - chr4_1:28071264-28071283 Msa0542730:CDS 40.0%
ATGAAAGTGATGAGAGAGAG+AGG + chr4_1:28071361-28071380 None:intergenic 40.0%
TCAGAAAGAGGATCAAACCA+AGG + chr4_1:28071681-28071700 None:intergenic 40.0%
!! GAATTTTGACAACACTGCAG+TGG + chr4_1:28072118-28072137 None:intergenic 40.0%
ATCTGTTGATGCAAAAGGAC+CGG - chr4_1:28072347-28072366 Msa0542730:intron 40.0%
AAATGATTTCTTGCTCCCTC+TGG - chr4_1:28072658-28072677 Msa0542730:intron 40.0%
AATGATTTCTTGCTCCCTCT+GGG - chr4_1:28072659-28072678 Msa0542730:intron 40.0%
CCATGGATACATAAAGCACA+TGG + chr4_1:28072902-28072921 None:intergenic 40.0%
CCATGTGCTTTATGTATCCA+TGG - chr4_1:28072899-28072918 Msa0542730:intron 40.0%
! ATGAGTTTGTTCGCTGTACT+AGG - chr4_1:28073234-28073253 Msa0542730:CDS 40.0%
ATGCTGTGTCAGATGCAAAT+TGG - chr4_1:28073315-28073334 Msa0542730:CDS 40.0%
!!! CTGGTTTTGTATGGTGAGTT+AGG + chr4_1:28073583-28073602 None:intergenic 40.0%
!!! CCTTACAAGCTGGTTTTGTA+TGG + chr4_1:28073592-28073611 None:intergenic 40.0%
CCATACAAAACCAGCTTGTA+AGG - chr4_1:28073589-28073608 Msa0542730:intron 40.0%
GTCTCACATTGGATGAGATA+TGG + chr4_1:28073653-28073672 None:intergenic 40.0%
GTGTGTTAAGAGTCTCACAT+TGG + chr4_1:28073664-28073683 None:intergenic 40.0%
CAGCACTCTGCAAATTAGTA+AGG + chr4_1:28073996-28074015 None:intergenic 40.0%
TGGAATAGTTTAACCAGACC+TGG - chr4_1:28074353-28074372 Msa0542730:intron 40.0%
ACACACAGAGAATCACTAGA+GGG + chr4_1:28074436-28074455 None:intergenic 40.0%
TACACACAGAGAATCACTAG+AGG + chr4_1:28074437-28074456 None:intergenic 40.0%
TGTTCAACTACTGAATGGGA+AGG - chr4_1:28074785-28074804 Msa0542730:intron 40.0%
AATTTGGGCACATTTCCTCA+AGG - chr4_1:28074839-28074858 Msa0542730:intron 40.0%
! TGTGATGATGAAGAAGAACG+AGG - chr4_1:28075039-28075058 Msa0542730:intron 40.0%
GGCAGTTGCAATCTGAAAAA+CGG + chr4_1:28075149-28075168 None:intergenic 40.0%
TGGTAAAGTCAGTACAAGTC+TGG + chr4_1:28075170-28075189 None:intergenic 40.0%
CAGACTGTTGATGTCCAATT+AGG + chr4_1:28075306-28075325 None:intergenic 40.0%
CTTTCACTGTTCTCATTGAC+TGG - chr4_1:28075333-28075352 Msa0542730:five_prime_UTR 40.0%
GGAGAAAATGGAGAATGGGT+TGG - chr4_1:28071126-28071145 Msa0542730:three_prime_UTR 45.0%
!! AATGGAGAATGGGTTGGAGA+AGG - chr4_1:28071132-28071151 Msa0542730:three_prime_UTR 45.0%
!! ATGGAGAATGGGTTGGAGAA+GGG - chr4_1:28071133-28071152 Msa0542730:three_prime_UTR 45.0%
!! AATGGGTTGGAGAAGGGAAA+TGG - chr4_1:28071139-28071158 Msa0542730:three_prime_UTR 45.0%
!! ATGGGTTGGAGAAGGGAAAT+GGG - chr4_1:28071140-28071159 Msa0542730:three_prime_UTR 45.0%
TGGGTTGTCAATGTTTAGCG+AGG - chr4_1:28071159-28071178 Msa0542730:three_prime_UTR 45.0%
GAAGAGCTGAGAGAAATGAG+TGG - chr4_1:28071181-28071200 Msa0542730:CDS 45.0%
AGAGCTGAGAGAAATGAGTG+GGG - chr4_1:28071183-28071202 Msa0542730:CDS 45.0%
! GTGATGCTGTTGGTAGACTT+AGG - chr4_1:28071263-28071282 Msa0542730:CDS 45.0%
TGTAACCGTAGTAAGGTCCT+AGG - chr4_1:28071853-28071872 Msa0542730:intron 45.0%
ACAACACTGCAGTGGATACA+TGG + chr4_1:28072110-28072129 None:intergenic 45.0%
GTGTTGTCAAAATTCCGCCA+TGG - chr4_1:28072123-28072142 Msa0542730:intron 45.0%
!!! TATGGCGGATTTTTGACCTG+TGG - chr4_1:28072239-28072258 Msa0542730:intron 45.0%
! TCATTTGTCAGCAGAGGTAC+CGG + chr4_1:28072369-28072388 None:intergenic 45.0%
TTGTTTGCCACATTGCAGGA+TGG - chr4_1:28072427-28072446 Msa0542730:intron 45.0%
GAGTGTTTCTCAAATGCAGC+AGG + chr4_1:28073053-28073072 None:intergenic 45.0%
GCTGCATTTGAGAAACACTC+TGG - chr4_1:28073053-28073072 Msa0542730:CDS 45.0%
GAAGAGAGACAGCAAGGAAA+TGG - chr4_1:28073075-28073094 Msa0542730:CDS 45.0%
! AGCAGCACTGTTTTACCCAA+TGG + chr4_1:28073140-28073159 None:intergenic 45.0%
TACAGCGAACAAACTCATCG+CGG + chr4_1:28073232-28073251 None:intergenic 45.0%
ACACAGCATCATGGTGAATG+CGG + chr4_1:28073304-28073323 None:intergenic 45.0%
TTGCATCTGACACAGCATCA+TGG + chr4_1:28073313-28073332 None:intergenic 45.0%
!!! CGCTCTAGATGTCTGGTTTT+GGG + chr4_1:28073697-28073716 None:intergenic 45.0%
!! ACGCTCTAGATGTCTGGTTT+TGG + chr4_1:28073698-28073717 None:intergenic 45.0%
! CAGACCTGGTACTGAATTCT+GGG - chr4_1:28074367-28074386 Msa0542730:intron 45.0%
CAAACCCAGAATTCAGTACC+AGG + chr4_1:28074374-28074393 None:intergenic 45.0%
GAATGGGAAGGACCAACTTA+TGG - chr4_1:28074797-28074816 Msa0542730:intron 45.0%
GGAAGTCGAAGAGTTTACAG+AGG - chr4_1:28075060-28075079 Msa0542730:CDS 45.0%
CGAAGAGTTTACAGAGGATG+CGG - chr4_1:28075066-28075085 Msa0542730:CDS 45.0%
!! AACCTTTGCACGTTGGTGAT+CGG + chr4_1:28075092-28075111 None:intergenic 45.0%
TGCACTTCTTGTGTGTGCTT+CGG - chr4_1:28075111-28075130 Msa0542730:CDS 45.0%
! ACACAACGCTGTTGTTGTTG+AGG + chr4_1:28075218-28075237 None:intergenic 45.0%
!! AAGATTATATATTATATTAT+AGG + chr4_1:28073767-28073786 None:intergenic 5.0%
TGCACTCCTGGTTGTGATGA+AGG - chr4_1:28071322-28071341 Msa0542730:CDS 50.0%
AGAGAGAGAGGAGAGAGAGA+GGG + chr4_1:28071349-28071368 None:intergenic 50.0%
!!! CACTATGGCGGATTTTGTGC+TGG - chr4_1:28072149-28072168 Msa0542730:intron 50.0%
ATAGATGTTGCGGCCTCTGA+CGG + chr4_1:28072175-28072194 None:intergenic 50.0%
! TATCAATGATGGCTGGCCAC+AGG + chr4_1:28072258-28072277 None:intergenic 50.0%
CTTGACACCATCCTGCAATG+TGG + chr4_1:28072437-28072456 None:intergenic 50.0%
! CACATTGCAGGATGGTGTCA+AGG - chr4_1:28072435-28072454 Msa0542730:intron 50.0%
!!! TCCCTCTGGGAGTGTTTTGA+GGG - chr4_1:28072672-28072691 Msa0542730:intron 50.0%
! ACTCTGGAAGAGAGACAGCA+AGG - chr4_1:28073069-28073088 Msa0542730:CDS 50.0%
CAAGTGGCGAAAGCTGCTAA+TGG - chr4_1:28073173-28073192 Msa0542730:CDS 50.0%
GGCTCTCAGAGATCCCAAAA+TGG - chr4_1:28073194-28073213 Msa0542730:CDS 50.0%
CTCAGAGATCCCAAAATGGC+CGG - chr4_1:28073198-28073217 Msa0542730:CDS 50.0%
TCAGAGATCCCAAAATGGCC+GGG - chr4_1:28073199-28073218 Msa0542730:CDS 50.0%
GCATCACTCACCTTACAAGC+TGG + chr4_1:28073602-28073621 None:intergenic 50.0%
!!! TCTGGTTTTGGGTGTGAGAG+GGG + chr4_1:28073686-28073705 None:intergenic 50.0%
!!! GTCTGGTTTTGGGTGTGAGA+GGG + chr4_1:28073687-28073706 None:intergenic 50.0%
!!! TGTCTGGTTTTGGGTGTGAG+AGG + chr4_1:28073688-28073707 None:intergenic 50.0%
! CAAGTCACGCTCTAGATGTC+TGG + chr4_1:28073704-28073723 None:intergenic 50.0%
! CAGACATCTAGAGCGTGACT+TGG - chr4_1:28073702-28073721 Msa0542730:intron 50.0%
! CATCTAGAGCGTGACTTGGA+TGG - chr4_1:28073706-28073725 Msa0542730:intron 50.0%
GACTTGGATGGTTTGATCGC+AGG - chr4_1:28073718-28073737 Msa0542730:intron 50.0%
TTGGATGGTTTGATCGCAGG+TGG - chr4_1:28073721-28073740 Msa0542730:intron 50.0%
CCAGAATTCAGTACCAGGTC+TGG + chr4_1:28074369-28074388 None:intergenic 50.0%
! CCAGACCTGGTACTGAATTC+TGG - chr4_1:28074366-28074385 Msa0542730:intron 50.0%
GAAGTGCAACCTTTGCACGT+TGG + chr4_1:28075099-28075118 None:intergenic 50.0%
GCACTTCTTGTGTGTGCTTC+GGG - chr4_1:28075112-28075131 Msa0542730:CDS 50.0%
GAGGGACCTTCATCACAACC+AGG + chr4_1:28071331-28071350 None:intergenic 55.0%
GAGAGAGAGAGGAGAGAGAG+AGG + chr4_1:28071350-28071369 None:intergenic 55.0%
ATTCCGCCATGGCAGCACTA+TGG - chr4_1:28072134-28072153 Msa0542730:intron 55.0%
AATCCGCCATAGTGCTGCCA+TGG + chr4_1:28072143-28072162 None:intergenic 55.0%
!! GATTTTGTGCTGGCCGTCAG+AGG - chr4_1:28072159-28072178 Msa0542730:intron 55.0%
!!! CTCCCTCTGGGAGTGTTTTG+AGG - chr4_1:28072671-28072690 Msa0542730:intron 55.0%
CCCCTCAAAACACTCCCAGA+GGG + chr4_1:28072676-28072695 None:intergenic 55.0%
!!! CCCTCTGGGAGTGTTTTGAG+GGG - chr4_1:28072673-28072692 Msa0542730:intron 55.0%
TCCCCTCAAAACACTCCCAG+AGG + chr4_1:28072677-28072696 None:intergenic 55.0%
AGATCCCAAAATGGCCGGGT+TGG - chr4_1:28073203-28073222 Msa0542730:CDS 55.0%
!! GTGACCAACCCGGCCATTTT+GGG + chr4_1:28073210-28073229 None:intergenic 55.0%
GCAGGTGGCCCAACAAATCA+TGG - chr4_1:28073736-28073755 Msa0542730:intron 55.0%
!! GGACACTGACACACCGACTC+AGG + chr4_1:28071531-28071550 None:intergenic 60.0%
! GGTGACCAACCCGGCCATTT+TGG + chr4_1:28073211-28073230 None:intergenic 60.0%
ACTCATCGCGGTGACCAACC+CGG + chr4_1:28073220-28073239 None:intergenic 60.0%
GGCCGATCACCAACGTGCAA+AGG - chr4_1:28075087-28075106 Msa0542730:CDS 60.0%
CCGCCATAGTGCTGCCATGG+CGG + chr4_1:28072140-28072159 None:intergenic 65.0%
CCGCCATGGCAGCACTATGG+CGG - chr4_1:28072137-28072156 Msa0542730:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 28070965 28075421 28070965 ID=Msa0542730;Name=Msa0542730
chr4_1 mRNA 28070965 28075421 28070965 ID=Msa0542730-mRNA-1;Parent=Msa0542730;Name=Msa0542730-mRNA-1;_AED=0.05;_eAED=0.05;_QI=159|1|1|1|0.5|0.66|3|215|239
chr4_1 exon 28070965 28071354 28070965 ID=Msa0542730-mRNA-1:exon:7583;Parent=Msa0542730-mRNA-1
chr4_1 exon 28073023 28073347 28073023 ID=Msa0542730-mRNA-1:exon:7582;Parent=Msa0542730-mRNA-1
chr4_1 exon 28075043 28075421 28075043 ID=Msa0542730-mRNA-1:exon:7581;Parent=Msa0542730-mRNA-1
chr4_1 five_prime_UTR 28075263 28075421 28075263 ID=Msa0542730-mRNA-1:five_prime_utr;Parent=Msa0542730-mRNA-1
chr4_1 CDS 28075043 28075262 28075043 ID=Msa0542730-mRNA-1:cds;Parent=Msa0542730-mRNA-1
chr4_1 CDS 28073023 28073347 28073023 ID=Msa0542730-mRNA-1:cds;Parent=Msa0542730-mRNA-1
chr4_1 CDS 28071180 28071354 28071180 ID=Msa0542730-mRNA-1:cds;Parent=Msa0542730-mRNA-1
chr4_1 three_prime_UTR 28070965 28071179 28070965 ID=Msa0542730-mRNA-1:three_prime_utr;Parent=Msa0542730-mRNA-1
Gene Sequence

>Msa0542730

ATGGAGAAAATGGAGAATGGGTTGGAGAAGGGAAATGGGTTGTCAATGTTTAGCGAGGAAGAGCTGAGAGAAATGAGTGGGGTTAGTGTTTTTGATGAATATGTTGAAGTCACGTGTGGGTGTACTAGTCATAGATATGGTGATGCTGTTGGTAGACTTAGGGTTTTTCTTAGTGGCTATCTTCAAATTATTTGTGAATGCACTCCTGGTTGTGATGAAGACAAGTTGACACCTGCTGCATTTGAGAAACACTCTGGAAGAGAGACAGCAAGGAAATGGAAGAATAATATCTGGGTTATAATTAATGGTGATAAGGTTCCATTGGGTAAAACAGTGCTGCTCAAATACTACAATCAAGTGGCGAAAGCTGCTAATGGCTCTCAGAGATCCCAAAATGGCCGGGTTGGTCACCGCGATGAGTTTGTTCGCTGTACTAGGTGCAACAAAGAGCGTAGATTTCGTCTGAGATCTAAAGAGGAATGCCGCATTCACCATGATGCTGTGTCAGATGCAAATTGGAAATGTTCTGATCTTCCATATGAGAAGATAGCATGTGATGATGAAGAAGAACGAGGAAGTCGAAGAGTTTACAGAGGATGCGGCCGATCACCAACGTGCAAAGGTTGCACTTCTTGTGTGTGCTTCGGGTGTGACATCTGCCGTTTTTCAGATTGCAACTGCCAGACTTGTACTGACTTTACCAGCAATGTAAAAGCTTGA
Protein sequence

>Msa0542730

MEKMENGLEKGNGLSMFSEEELREMSGVSVFDEYVEVTCGCTSHRYGDAVGRLRVFLSGYLQIICECTPGCDEDKLTPAAFEKHSGRETARKWKNNIWVIINGDKVPLGKTVLLKYYNQVAKAANGSQRSQNGRVGHRDEFVRCTRCNKERRFRLRSKEECRIHHDAVSDANWKCSDLPYEKIACDDEEERGSRRVYRGCGRSPTCKGCTSCVCFGCDICRFSDCNCQTCTDFTSNVKA*