AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0543760


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543760 MtrunA17_Chr4g0024181 87.838 74 9 0 1 74 1 74 2.27e-43 134
Msa0543760 MtrunA17_Chr2g0322831 70.000 50 15 0 24 73 30 79 4.25e-20 76.3
Msa0543760 MtrunA17_Chr2g0288361 49.333 75 35 2 1 73 1 74 5.28e-18 70.5
Msa0543760 MtrunA17_Chr5g0396771 65.217 46 16 0 26 71 37 82 5.89e-18 70.9
Msa0543760 MtrunA17_Chr7g0229101 57.143 42 18 0 27 68 29 70 4.52e-13 60.8
Msa0543760 MtrunA17_Chr4g0044421 58.537 41 17 0 28 68 70 110 1.19e-12 59.7
Msa0543760 MtrunA17_Chr4g0055981 63.415 41 15 0 28 68 113 153 2.52e-12 59.3
Msa0543760 MtrunA17_Chr4g0037141 58.537 41 17 0 28 68 70 110 3.01e-12 59.7
Msa0543760 MtrunA17_Chr8g0360111 58.537 41 17 0 28 68 83 123 3.48e-12 59.3
Msa0543760 MtrunA17_Chr5g0435101 56.098 41 18 0 28 68 69 109 3.78e-12 58.5
Msa0543760 MtrunA17_Chr7g0240821 54.545 44 20 0 28 71 59 102 4.32e-12 58.5
Msa0543760 MtrunA17_Chr3g0121241 57.143 42 18 0 27 68 99 140 6.24e-12 58.9
Msa0543760 MtrunA17_Chr4g0070641 58.537 41 17 0 28 68 70 110 8.27e-12 57.0
Msa0543760 MtrunA17_Chr4g0070611 57.500 40 16 1 29 68 72 110 1.18e-11 58.2
Msa0543760 MtrunA17_Chr7g0275911 57.143 42 17 1 30 71 73 113 2.19e-11 57.0
Msa0543760 MtrunA17_Chr7g0220341 56.098 41 18 0 28 68 85 125 2.22e-11 57.0
Msa0543760 MtrunA17_Chr8g0346561 56.098 41 18 0 28 68 70 110 2.28e-11 57.0
Msa0543760 MtrunA17_Chr3g0135311 50.000 42 21 0 28 69 71 112 6.68e-11 55.8
Msa0543760 MtrunA17_Chr1g0152011 52.381 42 20 0 28 69 71 112 6.77e-11 55.8
Msa0543760 MtrunA17_Chr5g0434901 51.220 41 20 0 28 68 31 71 6.92e-11 53.5
Msa0543760 MtrunA17_Chr7g0228991 51.220 41 20 0 28 68 87 127 8.02e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543760 AT2G46410.1 65.957 47 16 0 26 72 36 82 1.05e-16 68.2
Msa0543760 AT1G01380.1 68.293 41 13 0 26 66 32 72 1.64e-15 64.7
Msa0543760 AT5G53200.1 58.696 46 19 0 26 71 31 76 4.49e-14 62.0
Msa0543760 AT4G01060.3 63.415 41 15 0 26 66 31 71 1.02e-13 60.1
Msa0543760 AT4G01060.1 63.415 41 15 0 26 66 33 73 1.08e-13 60.1
Msa0543760 AT4G01060.2 63.415 41 15 0 26 66 30 70 1.09e-13 60.1
Msa0543760 AT2G30420.1 56.522 46 20 0 26 71 38 83 1.83e-12 57.8
Msa0543760 AT1G22640.3 58.537 41 17 0 28 68 13 53 3.20e-12 58.9
Msa0543760 AT4G38620.1 58.537 41 17 0 28 68 70 110 4.81e-12 59.3
Msa0543760 AT1G22640.1 52.000 50 23 1 19 68 62 110 6.56e-12 58.9
Msa0543760 AT5G14750.4 52.273 44 21 0 28 71 13 56 1.06e-11 56.6
Msa0543760 AT5G14750.2 52.273 44 21 0 28 71 13 56 1.06e-11 56.6
Msa0543760 AT5G35550.2 62.500 40 14 1 29 68 77 115 1.56e-11 57.8
Msa0543760 AT3G49690.1 54.762 42 19 0 28 69 71 112 2.04e-11 57.8
Msa0543760 AT4G09460.1 51.220 41 20 0 28 68 70 110 2.16e-11 57.4
Msa0543760 AT5G14750.3 52.273 44 21 0 28 71 40 83 2.24e-11 56.2
Msa0543760 AT3G27920.1 50.000 48 24 0 24 71 68 115 2.56e-11 57.0
Msa0543760 AT3G13540.1 58.537 41 17 0 28 68 81 121 3.37e-11 56.6
Msa0543760 AT2G30432.1 52.174 46 22 0 26 71 33 78 3.84e-11 53.9
Msa0543760 AT5G14750.1 52.273 44 21 0 28 71 74 117 4.06e-11 56.2
Msa0543760 AT2G30424.1 50.000 46 23 0 26 71 34 79 5.24e-11 53.9
Msa0543760 AT5G65790.1 52.381 42 20 0 28 69 71 112 6.67e-11 56.2

Find 27 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGGAAATGCTTATTAAAA+TGG 0.190572 4_1:+30793883 Msa0543760:CDS
ATCTCTTCTGCTGTTCTTCC+AGG 0.213845 4_1:-30794238 None:intergenic
ACTACAAATATAGAAGAAAT+AGG 0.233114 4_1:-30793470 None:intergenic
AAATGGTTTATAATCTTGTT+GGG 0.339232 4_1:+30793900 Msa0543760:CDS
AAGTAAATTATTGTTAATAT+AGG 0.342014 4_1:-30793520 None:intergenic
TTGATTGCTGGAAGAATTCC+TGG 0.342209 4_1:+30794220 Msa0543760:CDS
TGAGTGGTGTATTAATATAT+AGG 0.379554 4_1:-30793546 None:intergenic
AAAATGGTTTATAATCTTGT+TGG 0.380870 4_1:+30793899 Msa0543760:CDS
CATAGGTGGTCATTGATTGC+TGG 0.399125 4_1:+30794208 Msa0543760:intron
TCAACCTTTGAACCTTGGTC+TGG 0.418134 4_1:-30793848 None:intergenic
CTACAAATATAGAAGAAATA+GGG 0.428239 4_1:-30793469 None:intergenic
AATCAAGATGTGGGAAACTA+TGG 0.433656 4_1:-30793602 None:intergenic
ATAGAAGAAATAGGGAAGAC+GGG 0.441257 4_1:-30793461 None:intergenic
AAAATTCAACCTTTGAACCT+TGG 0.444402 4_1:-30793853 None:intergenic
TTTCTTGATTCATTCATAGC+TGG 0.462473 4_1:-30794305 None:intergenic
TTTATAATCTTGTTGGGGAG+AGG 0.507013 4_1:+30793906 Msa0543760:CDS
CTATTTGATAATCAAGATGT+GGG 0.528349 4_1:-30793611 None:intergenic
TATAGAAGAAATAGGGAAGA+CGG 0.528636 4_1:-30793462 None:intergenic
TCTATTTGATAATCAAGATG+TGG 0.534329 4_1:-30793612 None:intergenic
AACTTAATTTGCTATTGCAA+TGG 0.540183 4_1:+30793650 Msa0543760:exon
TGAATGAATCAAGAAATTTG+TGG 0.548018 4_1:+30794312 Msa0543760:three_prime_UTR
AATGGTTTATAATCTTGTTG+GGG 0.563787 4_1:+30793901 Msa0543760:CDS
GCAACCAGACCAAGGTTCAA+AGG 0.564494 4_1:+30793844 Msa0543760:CDS
AATGTTTCAACAGATTCCAA+AGG 0.613753 4_1:+30793696 Msa0543760:CDS
ACAGATCAGCAACCAGACCA+AGG 0.650716 4_1:+30793836 Msa0543760:intron
TAGAAGAAATAGGGAAGACG+GGG 0.665985 4_1:-30793460 None:intergenic
TGGAATGATTGAGATTTGAG+TGG 0.693035 4_1:-30793562 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAATTATTGTTAATAT+AGG - chr4_1:30793523-30793542 None:intergenic 10.0%
!! AAAAAAAAAGTTACAATTTA+AGG - chr4_1:30793940-30793959 None:intergenic 10.0%
!! AAGTAAATTATTGTTAATAT+AGG - chr4_1:30793523-30793542 None:intergenic 10.0%
!! AAAAAAAAAGTTACAATTTA+AGG - chr4_1:30793940-30793959 None:intergenic 10.0%
!! CTACAAATATAGAAGAAATA+GGG - chr4_1:30793472-30793491 None:intergenic 20.0%
!! ACTACAAATATAGAAGAAAT+AGG - chr4_1:30793473-30793492 None:intergenic 20.0%
!! ATTTCTCTTAGTCAATAAAA+TGG - chr4_1:30793804-30793823 None:intergenic 20.0%
!!! ATTTTATTGACTAAGAGAAA+TGG + chr4_1:30793803-30793822 Msa0543760:intron 20.0%
!! AAAATGGTTTATAATCTTGT+TGG + chr4_1:30793899-30793918 Msa0543760:CDS 20.0%
!! AAATGGTTTATAATCTTGTT+GGG + chr4_1:30793900-30793919 Msa0543760:CDS 20.0%
!! ATAACATCAATAACAAAGTT+TGG - chr4_1:30793982-30794001 None:intergenic 20.0%
!! TATGAATGAATCAAGAAATT+TGG + chr4_1:30794310-30794329 Msa0543760:three_prime_UTR 20.0%
!! CTACAAATATAGAAGAAATA+GGG - chr4_1:30793472-30793491 None:intergenic 20.0%
!! ACTACAAATATAGAAGAAAT+AGG - chr4_1:30793473-30793492 None:intergenic 20.0%
!! ATTTCTCTTAGTCAATAAAA+TGG - chr4_1:30793804-30793823 None:intergenic 20.0%
!!! ATTTTATTGACTAAGAGAAA+TGG + chr4_1:30793803-30793822 Msa0543760:intron 20.0%
!! AAAATGGTTTATAATCTTGT+TGG + chr4_1:30793899-30793918 Msa0543760:CDS 20.0%
!! AAATGGTTTATAATCTTGTT+GGG + chr4_1:30793900-30793919 Msa0543760:CDS 20.0%
!! ATAACATCAATAACAAAGTT+TGG - chr4_1:30793982-30794001 None:intergenic 20.0%
! TGAGTGGTGTATTAATATAT+AGG - chr4_1:30793549-30793568 None:intergenic 25.0%
!! CTATTTGATAATCAAGATGT+GGG - chr4_1:30793614-30793633 None:intergenic 25.0%
!! TCTATTTGATAATCAAGATG+TGG - chr4_1:30793615-30793634 None:intergenic 25.0%
!!! AACTTAATTTGCTATTGCAA+TGG + chr4_1:30793650-30793669 Msa0543760:exon 25.0%
!!! ATCTGTTGAAACATTTTTAG+AGG - chr4_1:30793691-30793710 None:intergenic 25.0%
!! AAAAGACATGCATTCTAAAA+GGG - chr4_1:30793724-30793743 None:intergenic 25.0%
! TAAAAGACATGCATTCTAAA+AGG - chr4_1:30793725-30793744 None:intergenic 25.0%
! AGAGGAAATGCTTATTAAAA+TGG + chr4_1:30793883-30793902 Msa0543760:CDS 25.0%
! AATGGTTTATAATCTTGTTG+GGG + chr4_1:30793901-30793920 Msa0543760:CDS 25.0%
!! ATGAATGTGTATTTTCTCAT+AGG + chr4_1:30794191-30794210 Msa0543760:intron 25.0%
! CAGAAGAGATAGAAAAATAT+TGG + chr4_1:30794251-30794270 Msa0543760:CDS 25.0%
! TGAGTGGTGTATTAATATAT+AGG - chr4_1:30793549-30793568 None:intergenic 25.0%
!! CTATTTGATAATCAAGATGT+GGG - chr4_1:30793614-30793633 None:intergenic 25.0%
!! TCTATTTGATAATCAAGATG+TGG - chr4_1:30793615-30793634 None:intergenic 25.0%
!!! AACTTAATTTGCTATTGCAA+TGG + chr4_1:30793650-30793669 Msa0543760:exon 25.0%
!!! ATCTGTTGAAACATTTTTAG+AGG - chr4_1:30793691-30793710 None:intergenic 25.0%
!! AAAAGACATGCATTCTAAAA+GGG - chr4_1:30793724-30793743 None:intergenic 25.0%
! TAAAAGACATGCATTCTAAA+AGG - chr4_1:30793725-30793744 None:intergenic 25.0%
! AGAGGAAATGCTTATTAAAA+TGG + chr4_1:30793883-30793902 Msa0543760:CDS 25.0%
! AATGGTTTATAATCTTGTTG+GGG + chr4_1:30793901-30793920 Msa0543760:CDS 25.0%
!! ATGAATGTGTATTTTCTCAT+AGG + chr4_1:30794191-30794210 Msa0543760:intron 25.0%
! CAGAAGAGATAGAAAAATAT+TGG + chr4_1:30794251-30794270 Msa0543760:CDS 25.0%
TATAGAAGAAATAGGGAAGA+CGG - chr4_1:30793465-30793484 None:intergenic 30.0%
!!! TGGTGTTATTTTGCAGAATA+TGG - chr4_1:30793585-30793604 None:intergenic 30.0%
AATGTTTCAACAGATTCCAA+AGG + chr4_1:30793696-30793715 Msa0543760:CDS 30.0%
AAAATTCAACCTTTGAACCT+TGG - chr4_1:30793856-30793875 None:intergenic 30.0%
! TTTGGAATGAAAGTGAAAAG+AGG - chr4_1:30793964-30793983 None:intergenic 30.0%
GTCTAGTGTTTGTGTTTATT+TGG - chr4_1:30794018-30794037 None:intergenic 30.0%
! AATGTGTATTTTCTCATAGG+TGG + chr4_1:30794194-30794213 Msa0543760:intron 30.0%
TTTCTTGATTCATTCATAGC+TGG - chr4_1:30794308-30794327 None:intergenic 30.0%
TATAGAAGAAATAGGGAAGA+CGG - chr4_1:30793465-30793484 None:intergenic 30.0%
!!! TGGTGTTATTTTGCAGAATA+TGG - chr4_1:30793585-30793604 None:intergenic 30.0%
AATGTTTCAACAGATTCCAA+AGG + chr4_1:30793696-30793715 Msa0543760:CDS 30.0%
AAAATTCAACCTTTGAACCT+TGG - chr4_1:30793856-30793875 None:intergenic 30.0%
! TTTGGAATGAAAGTGAAAAG+AGG - chr4_1:30793964-30793983 None:intergenic 30.0%
GTCTAGTGTTTGTGTTTATT+TGG - chr4_1:30794018-30794037 None:intergenic 30.0%
! AATGTGTATTTTCTCATAGG+TGG + chr4_1:30794194-30794213 Msa0543760:intron 30.0%
TTTCTTGATTCATTCATAGC+TGG - chr4_1:30794308-30794327 None:intergenic 30.0%
ATAGAAGAAATAGGGAAGAC+GGG - chr4_1:30793464-30793483 None:intergenic 35.0%
TGGAATGATTGAGATTTGAG+TGG - chr4_1:30793565-30793584 None:intergenic 35.0%
AATCAAGATGTGGGAAACTA+TGG - chr4_1:30793605-30793624 None:intergenic 35.0%
!! GGTTGAATTTTCTGAAGAAG+AGG + chr4_1:30793865-30793884 Msa0543760:CDS 35.0%
TTTATAATCTTGTTGGGGAG+AGG + chr4_1:30793906-30793925 Msa0543760:CDS 35.0%
TAACATGCATGTATTCGTGT+TGG + chr4_1:30794072-30794091 Msa0543760:intron 35.0%
ATAGAAGAAATAGGGAAGAC+GGG - chr4_1:30793464-30793483 None:intergenic 35.0%
TGGAATGATTGAGATTTGAG+TGG - chr4_1:30793565-30793584 None:intergenic 35.0%
AATCAAGATGTGGGAAACTA+TGG - chr4_1:30793605-30793624 None:intergenic 35.0%
!! GGTTGAATTTTCTGAAGAAG+AGG + chr4_1:30793865-30793884 Msa0543760:CDS 35.0%
TTTATAATCTTGTTGGGGAG+AGG + chr4_1:30793906-30793925 Msa0543760:CDS 35.0%
TAACATGCATGTATTCGTGT+TGG + chr4_1:30794072-30794091 Msa0543760:intron 35.0%
TAGAAGAAATAGGGAAGACG+GGG - chr4_1:30793463-30793482 None:intergenic 40.0%
!! GCATTCTAAAAGGGTACCTT+TGG - chr4_1:30793715-30793734 None:intergenic 40.0%
! AAACCTTCGATCTAGAGTGT+CGG + chr4_1:30794121-30794140 Msa0543760:intron 40.0%
TTGATTGCTGGAAGAATTCC+TGG + chr4_1:30794220-30794239 Msa0543760:CDS 40.0%
TAGAAGAAATAGGGAAGACG+GGG - chr4_1:30793463-30793482 None:intergenic 40.0%
!! GCATTCTAAAAGGGTACCTT+TGG - chr4_1:30793715-30793734 None:intergenic 40.0%
! AAACCTTCGATCTAGAGTGT+CGG + chr4_1:30794121-30794140 Msa0543760:intron 40.0%
TTGATTGCTGGAAGAATTCC+TGG + chr4_1:30794220-30794239 Msa0543760:CDS 40.0%
TCAACCTTTGAACCTTGGTC+TGG - chr4_1:30793851-30793870 None:intergenic 45.0%
CTCTAGATCGAAGGTTTGTC+TGG - chr4_1:30794118-30794137 None:intergenic 45.0%
! CATAGGTGGTCATTGATTGC+TGG + chr4_1:30794208-30794227 Msa0543760:intron 45.0%
ATCTCTTCTGCTGTTCTTCC+AGG - chr4_1:30794241-30794260 None:intergenic 45.0%
TCAACCTTTGAACCTTGGTC+TGG - chr4_1:30793851-30793870 None:intergenic 45.0%
CTCTAGATCGAAGGTTTGTC+TGG - chr4_1:30794118-30794137 None:intergenic 45.0%
! CATAGGTGGTCATTGATTGC+TGG + chr4_1:30794208-30794227 Msa0543760:intron 45.0%
ATCTCTTCTGCTGTTCTTCC+AGG - chr4_1:30794241-30794260 None:intergenic 45.0%
ACAGATCAGCAACCAGACCA+AGG + chr4_1:30793836-30793855 Msa0543760:intron 50.0%
GCAACCAGACCAAGGTTCAA+AGG + chr4_1:30793844-30793863 Msa0543760:CDS 50.0%
ACACCGACACTCTAGATCGA+AGG - chr4_1:30794127-30794146 None:intergenic 50.0%
ACAGATCAGCAACCAGACCA+AGG + chr4_1:30793836-30793855 Msa0543760:intron 50.0%
GCAACCAGACCAAGGTTCAA+AGG + chr4_1:30793844-30793863 Msa0543760:CDS 50.0%
ACACCGACACTCTAGATCGA+AGG - chr4_1:30794127-30794146 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 30793451 30794331 30793451 ID=Msa0543760;Name=Msa0543760
chr4_1 mRNA 30793451 30794331 30793451 ID=Msa0543760-mRNA-1;Parent=Msa0543760;Name=Msa0543760-mRNA-1;_AED=0.09;_eAED=0.09;_QI=218|1|1|1|1|1|3|31|74
chr4_1 exon 30793451 30793717 30793451 ID=Msa0543760-mRNA-1:exon:8068;Parent=Msa0543760-mRNA-1
chr4_1 exon 30793840 30793927 30793840 ID=Msa0543760-mRNA-1:exon:8069;Parent=Msa0543760-mRNA-1
chr4_1 exon 30794213 30794331 30794213 ID=Msa0543760-mRNA-1:exon:8070;Parent=Msa0543760-mRNA-1
chr4_1 five_prime_UTR 30793451 30793668 30793451 ID=Msa0543760-mRNA-1:five_prime_utr;Parent=Msa0543760-mRNA-1
chr4_1 CDS 30793669 30793717 30793669 ID=Msa0543760-mRNA-1:cds;Parent=Msa0543760-mRNA-1
chr4_1 CDS 30793840 30793927 30793840 ID=Msa0543760-mRNA-1:cds;Parent=Msa0543760-mRNA-1
chr4_1 CDS 30794213 30794300 30794213 ID=Msa0543760-mRNA-1:cds;Parent=Msa0543760-mRNA-1
chr4_1 three_prime_UTR 30794301 30794331 30794301 ID=Msa0543760-mRNA-1:three_prime_utr;Parent=Msa0543760-mRNA-1
Gene Sequence

>Msa0543760

ATGGCTGACTCAGAACACTCCTCTAAAAATGTTTCAACAGATTCCAAAGATCAGCAACCAGACCAAGGTTCAAAGGTTGAATTTTCTGAAGAAGAGGAAATGCTTATTAAAATGGTTTATAATCTTGTTGGGGAGAGGTGGTCATTGATTGCTGGAAGAATTCCTGGAAGAACAGCAGAAGAGATAGAAAAATATTGGAATTCAAGACACTCGACAAGCGAATGA

Protein sequence

>Msa0543760

MADSEHSSKNVSTDSKDQQPDQGSKVEFSEEEEMLIKMVYNLVGERWSLIAGRIPGRTAEEIEKYWNSRHSTSE*