AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0543770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543770 MtrunA17_Chr4g0024181 86.486 74 10 0 1 74 1 74 7.35e-43 133
Msa0543770 MtrunA17_Chr2g0322831 70.000 50 15 0 24 73 30 79 4.20e-20 76.3
Msa0543770 MtrunA17_Chr5g0396771 65.217 46 16 0 26 71 37 82 5.23e-18 71.2
Msa0543770 MtrunA17_Chr2g0288361 49.333 75 35 2 1 73 1 74 5.52e-18 70.5
Msa0543770 MtrunA17_Chr7g0229101 57.143 42 18 0 27 68 29 70 4.82e-13 60.8
Msa0543770 MtrunA17_Chr4g0044421 58.537 41 17 0 28 68 70 110 1.12e-12 59.7
Msa0543770 MtrunA17_Chr4g0055981 63.415 41 15 0 28 68 113 153 2.50e-12 59.3
Msa0543770 MtrunA17_Chr4g0037141 58.537 41 17 0 28 68 70 110 2.81e-12 59.7
Msa0543770 MtrunA17_Chr8g0360111 58.537 41 17 0 28 68 83 123 3.51e-12 59.3
Msa0543770 MtrunA17_Chr5g0435101 56.098 41 18 0 28 68 69 109 3.67e-12 58.5
Msa0543770 MtrunA17_Chr7g0240821 54.545 44 20 0 28 71 59 102 4.51e-12 58.5
Msa0543770 MtrunA17_Chr3g0121241 57.143 42 18 0 27 68 99 140 6.00e-12 58.9
Msa0543770 MtrunA17_Chr4g0070641 58.537 41 17 0 28 68 70 110 7.60e-12 57.0
Msa0543770 MtrunA17_Chr4g0070611 57.500 40 16 1 29 68 72 110 1.16e-11 58.2
Msa0543770 MtrunA17_Chr8g0346561 56.098 41 18 0 28 68 70 110 2.21e-11 57.4
Msa0543770 MtrunA17_Chr7g0275911 57.143 42 17 1 30 71 73 113 2.21e-11 57.0
Msa0543770 MtrunA17_Chr7g0220341 56.098 41 18 0 28 68 85 125 2.24e-11 57.0
Msa0543770 MtrunA17_Chr3g0135311 50.000 42 21 0 28 69 71 112 6.42e-11 55.8
Msa0543770 MtrunA17_Chr1g0152011 52.381 42 20 0 28 69 71 112 6.45e-11 55.8
Msa0543770 MtrunA17_Chr5g0434901 51.220 41 20 0 28 68 31 71 7.00e-11 53.5
Msa0543770 MtrunA17_Chr7g0228991 51.220 41 20 0 28 68 87 127 8.19e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0543770 AT2G46410.1 65.957 47 16 0 26 72 36 82 9.82e-17 68.2
Msa0543770 AT1G01380.1 68.293 41 13 0 26 66 32 72 1.40e-15 65.1
Msa0543770 AT5G53200.1 58.696 46 19 0 26 71 31 76 4.11e-14 62.0
Msa0543770 AT4G01060.3 63.415 41 15 0 26 66 31 71 9.21e-14 60.1
Msa0543770 AT4G01060.1 63.415 41 15 0 26 66 33 73 9.89e-14 60.1
Msa0543770 AT4G01060.2 63.415 41 15 0 26 66 30 70 9.92e-14 60.1
Msa0543770 AT2G30420.1 56.522 46 20 0 26 71 38 83 1.68e-12 58.2
Msa0543770 AT1G22640.3 58.537 41 17 0 28 68 13 53 3.07e-12 58.9
Msa0543770 AT4G38620.1 58.537 41 17 0 28 68 70 110 4.81e-12 59.3
Msa0543770 AT1G22640.1 58.537 41 17 0 28 68 70 110 6.62e-12 58.9
Msa0543770 AT5G14750.4 52.273 44 21 0 28 71 13 56 1.11e-11 56.6
Msa0543770 AT5G14750.2 52.273 44 21 0 28 71 13 56 1.11e-11 56.6
Msa0543770 AT5G35550.2 62.500 40 14 1 29 68 77 115 1.84e-11 57.8
Msa0543770 AT3G49690.1 54.762 42 19 0 28 69 71 112 1.89e-11 57.8
Msa0543770 AT4G09460.1 51.220 41 20 0 28 68 70 110 2.23e-11 57.0
Msa0543770 AT5G14750.3 52.273 44 21 0 28 71 40 83 2.24e-11 56.2
Msa0543770 AT3G27920.1 50.000 48 24 0 24 71 68 115 2.83e-11 57.0
Msa0543770 AT2G30424.1 48.077 52 24 1 26 74 34 85 3.01e-11 54.3
Msa0543770 AT3G13540.1 58.537 41 17 0 28 68 81 121 3.17e-11 57.0
Msa0543770 AT2G30432.1 52.174 46 22 0 26 71 33 78 3.68e-11 53.9
Msa0543770 AT5G14750.1 52.273 44 21 0 28 71 74 117 4.28e-11 56.2
Msa0543770 AT5G65790.1 52.381 42 20 0 28 69 71 112 6.48e-11 56.6

Find 26 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGGAAATGCTTATTAAAA+TGG 0.190572 4_1:+30812302 Msa0543770:CDS
ATCTCTTCTGCTGTTCTTCC+AGG 0.213845 4_1:-30812658 None:intergenic
ACTAGAAATAGAGAAGAAAT+AGG 0.277297 4_1:-30811885 None:intergenic
AAATGGTTTATAATCTTGTT+GGG 0.339232 4_1:+30812319 Msa0543770:CDS
AAGTAAATTATTGTTAATAT+AGG 0.342014 4_1:-30811935 None:intergenic
TTGATTGCTGGAAGAATTCC+TGG 0.342209 4_1:+30812640 Msa0543770:CDS
TGAGTGGTGTATTAATATAT+AGG 0.379554 4_1:-30811961 None:intergenic
AAAATGGTTTATAATCTTGT+TGG 0.380870 4_1:+30812318 Msa0543770:CDS
CATAGGTGGTCATTGATTGC+TGG 0.399125 4_1:+30812628 Msa0543770:intron
TTTCTTGATTCATTCATAGT+TGG 0.417658 4_1:-30812725 None:intergenic
TCAACCTTTGAACCTTGGTC+TGG 0.418134 4_1:-30812267 None:intergenic
AGAGAAGAAATAGGGAAGAC+GGG 0.426905 4_1:-30811876 None:intergenic
AATCAAGATGTGGGAAACTA+TGG 0.433656 4_1:-30812017 None:intergenic
AAAATTCAACCTTTGAACCT+TGG 0.444402 4_1:-30812272 None:intergenic
CTAGAAATAGAGAAGAAATA+GGG 0.460374 4_1:-30811884 None:intergenic
TAGAGAAGAAATAGGGAAGA+CGG 0.468552 4_1:-30811877 None:intergenic
TTTATAATCTTGTTGGGGAG+AGG 0.507013 4_1:+30812325 Msa0543770:CDS
CTATTTGATAATCAAGATGT+GGG 0.528349 4_1:-30812026 None:intergenic
TCTATTTGATAATCAAGATG+TGG 0.534329 4_1:-30812027 None:intergenic
TGAATGAATCAAGAAATTTG+TGG 0.536313 4_1:+30812732 Msa0543770:three_prime_UTR
AATGGTTTATAATCTTGTTG+GGG 0.563787 4_1:+30812320 Msa0543770:CDS
GCAACCAGACCAAGGTTCAA+AGG 0.564494 4_1:+30812263 Msa0543770:CDS
AATGTTTCAACAGATTCCAA+AGG 0.606722 4_1:+30812114 Msa0543770:CDS
GAGAAGAAATAGGGAAGACG+GGG 0.635275 4_1:-30811875 None:intergenic
ACAGATCAGCAACCAGACCA+AGG 0.650716 4_1:+30812255 Msa0543770:intron
TGGAATGATTGAGATTTGAG+TGG 0.693035 4_1:-30811977 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAATTATTGTTAATAT+AGG - chr4_1:30811938-30811957 None:intergenic 10.0%
!! AAAAAAAAAGTTACAATTTA+AGG - chr4_1:30812359-30812378 None:intergenic 10.0%
!! AAGTAAATTATTGTTAATAT+AGG - chr4_1:30811938-30811957 None:intergenic 10.0%
!! AAAAAAAAAGTTACAATTTA+AGG - chr4_1:30812359-30812378 None:intergenic 10.0%
!!! TGTCTTTTATAGCTAATTAA+TGG + chr4_1:30812156-30812175 Msa0543770:intron 20.0%
!! ATTTCTCTTAGTCAATAAAA+TGG - chr4_1:30812223-30812242 None:intergenic 20.0%
!!! ATTTTATTGACTAAGAGAAA+TGG + chr4_1:30812222-30812241 Msa0543770:intron 20.0%
!! AAAATGGTTTATAATCTTGT+TGG + chr4_1:30812318-30812337 Msa0543770:CDS 20.0%
!! AAATGGTTTATAATCTTGTT+GGG + chr4_1:30812319-30812338 Msa0543770:CDS 20.0%
!! ATAACATCAATAACAAAGTT+TGG - chr4_1:30812401-30812420 None:intergenic 20.0%
!! TATGAATGAATCAAGAAATT+TGG + chr4_1:30812730-30812749 Msa0543770:three_prime_UTR 20.0%
!!! TGTCTTTTATAGCTAATTAA+TGG + chr4_1:30812156-30812175 Msa0543770:intron 20.0%
!! ATTTCTCTTAGTCAATAAAA+TGG - chr4_1:30812223-30812242 None:intergenic 20.0%
!!! ATTTTATTGACTAAGAGAAA+TGG + chr4_1:30812222-30812241 Msa0543770:intron 20.0%
!! AAAATGGTTTATAATCTTGT+TGG + chr4_1:30812318-30812337 Msa0543770:CDS 20.0%
!! AAATGGTTTATAATCTTGTT+GGG + chr4_1:30812319-30812338 Msa0543770:CDS 20.0%
!! ATAACATCAATAACAAAGTT+TGG - chr4_1:30812401-30812420 None:intergenic 20.0%
! CTAGAAATAGAGAAGAAATA+GGG - chr4_1:30811887-30811906 None:intergenic 25.0%
! ACTAGAAATAGAGAAGAAAT+AGG - chr4_1:30811888-30811907 None:intergenic 25.0%
! TGAGTGGTGTATTAATATAT+AGG - chr4_1:30811964-30811983 None:intergenic 25.0%
!! CTATTTGATAATCAAGATGT+GGG - chr4_1:30812029-30812048 None:intergenic 25.0%
!! TCTATTTGATAATCAAGATG+TGG - chr4_1:30812030-30812049 None:intergenic 25.0%
!! AAAAGACATGCATTCTAAAA+GGG - chr4_1:30812144-30812163 None:intergenic 25.0%
! TAAAAGACATGCATTCTAAA+AGG - chr4_1:30812145-30812164 None:intergenic 25.0%
! AGAGGAAATGCTTATTAAAA+TGG + chr4_1:30812302-30812321 Msa0543770:CDS 25.0%
! AATGGTTTATAATCTTGTTG+GGG + chr4_1:30812320-30812339 Msa0543770:CDS 25.0%
!! ATGAATGTGTATTTTCTCAT+AGG + chr4_1:30812611-30812630 Msa0543770:intron 25.0%
! CAGAAGAGATAGAAAAATAT+TGG + chr4_1:30812671-30812690 Msa0543770:CDS 25.0%
! TTTCTTGATTCATTCATAGT+TGG - chr4_1:30812728-30812747 None:intergenic 25.0%
! CTAGAAATAGAGAAGAAATA+GGG - chr4_1:30811887-30811906 None:intergenic 25.0%
! ACTAGAAATAGAGAAGAAAT+AGG - chr4_1:30811888-30811907 None:intergenic 25.0%
! TGAGTGGTGTATTAATATAT+AGG - chr4_1:30811964-30811983 None:intergenic 25.0%
!! CTATTTGATAATCAAGATGT+GGG - chr4_1:30812029-30812048 None:intergenic 25.0%
!! TCTATTTGATAATCAAGATG+TGG - chr4_1:30812030-30812049 None:intergenic 25.0%
!! AAAAGACATGCATTCTAAAA+GGG - chr4_1:30812144-30812163 None:intergenic 25.0%
! TAAAAGACATGCATTCTAAA+AGG - chr4_1:30812145-30812164 None:intergenic 25.0%
! AGAGGAAATGCTTATTAAAA+TGG + chr4_1:30812302-30812321 Msa0543770:CDS 25.0%
! AATGGTTTATAATCTTGTTG+GGG + chr4_1:30812320-30812339 Msa0543770:CDS 25.0%
!! ATGAATGTGTATTTTCTCAT+AGG + chr4_1:30812611-30812630 Msa0543770:intron 25.0%
! CAGAAGAGATAGAAAAATAT+TGG + chr4_1:30812671-30812690 Msa0543770:CDS 25.0%
! TTTCTTGATTCATTCATAGT+TGG - chr4_1:30812728-30812747 None:intergenic 25.0%
!!! TGGTGTTATTTTGCAGAATA+TGG - chr4_1:30812000-30812019 None:intergenic 30.0%
!! AACTCAATTTGCTTTTGCAA+TGG + chr4_1:30812068-30812087 Msa0543770:exon 30.0%
AATGTTTCAACAGATTCCAA+AGG + chr4_1:30812114-30812133 Msa0543770:CDS 30.0%
! ATTCTAAAAGGGTATACCTT+TGG - chr4_1:30812133-30812152 None:intergenic 30.0%
AAAATTCAACCTTTGAACCT+TGG - chr4_1:30812275-30812294 None:intergenic 30.0%
! TTTGGAATGAAAGTGAAAAG+AGG - chr4_1:30812383-30812402 None:intergenic 30.0%
GTCTAGTGTTTGTGTTTATT+TGG - chr4_1:30812437-30812456 None:intergenic 30.0%
! AATGTGTATTTTCTCATAGG+TGG + chr4_1:30812614-30812633 Msa0543770:intron 30.0%
!!! TGGTGTTATTTTGCAGAATA+TGG - chr4_1:30812000-30812019 None:intergenic 30.0%
!! AACTCAATTTGCTTTTGCAA+TGG + chr4_1:30812068-30812087 Msa0543770:exon 30.0%
AATGTTTCAACAGATTCCAA+AGG + chr4_1:30812114-30812133 Msa0543770:CDS 30.0%
! ATTCTAAAAGGGTATACCTT+TGG - chr4_1:30812133-30812152 None:intergenic 30.0%
AAAATTCAACCTTTGAACCT+TGG - chr4_1:30812275-30812294 None:intergenic 30.0%
! TTTGGAATGAAAGTGAAAAG+AGG - chr4_1:30812383-30812402 None:intergenic 30.0%
GTCTAGTGTTTGTGTTTATT+TGG - chr4_1:30812437-30812456 None:intergenic 30.0%
! AATGTGTATTTTCTCATAGG+TGG + chr4_1:30812614-30812633 Msa0543770:intron 30.0%
TAGAGAAGAAATAGGGAAGA+CGG - chr4_1:30811880-30811899 None:intergenic 35.0%
TGGAATGATTGAGATTTGAG+TGG - chr4_1:30811980-30811999 None:intergenic 35.0%
AATCAAGATGTGGGAAACTA+TGG - chr4_1:30812020-30812039 None:intergenic 35.0%
!! GGTTGAATTTTCTGAAGAAG+AGG + chr4_1:30812284-30812303 Msa0543770:CDS 35.0%
TTTATAATCTTGTTGGGGAG+AGG + chr4_1:30812325-30812344 Msa0543770:CDS 35.0%
AACACTGCATGTATTCATGT+TGG + chr4_1:30812492-30812511 Msa0543770:intron 35.0%
TAGAGAAGAAATAGGGAAGA+CGG - chr4_1:30811880-30811899 None:intergenic 35.0%
TGGAATGATTGAGATTTGAG+TGG - chr4_1:30811980-30811999 None:intergenic 35.0%
AATCAAGATGTGGGAAACTA+TGG - chr4_1:30812020-30812039 None:intergenic 35.0%
!! GGTTGAATTTTCTGAAGAAG+AGG + chr4_1:30812284-30812303 Msa0543770:CDS 35.0%
TTTATAATCTTGTTGGGGAG+AGG + chr4_1:30812325-30812344 Msa0543770:CDS 35.0%
AACACTGCATGTATTCATGT+TGG + chr4_1:30812492-30812511 Msa0543770:intron 35.0%
AGAGAAGAAATAGGGAAGAC+GGG - chr4_1:30811879-30811898 None:intergenic 40.0%
! AAACCTTCGATCTAGAGTGT+CGG + chr4_1:30812541-30812560 Msa0543770:intron 40.0%
TTGATTGCTGGAAGAATTCC+TGG + chr4_1:30812640-30812659 Msa0543770:CDS 40.0%
AGAGAAGAAATAGGGAAGAC+GGG - chr4_1:30811879-30811898 None:intergenic 40.0%
! AAACCTTCGATCTAGAGTGT+CGG + chr4_1:30812541-30812560 Msa0543770:intron 40.0%
TTGATTGCTGGAAGAATTCC+TGG + chr4_1:30812640-30812659 Msa0543770:CDS 40.0%
GAGAAGAAATAGGGAAGACG+GGG - chr4_1:30811878-30811897 None:intergenic 45.0%
TCAACCTTTGAACCTTGGTC+TGG - chr4_1:30812270-30812289 None:intergenic 45.0%
CTCTAGATCGAAGGTTTGTC+TGG - chr4_1:30812538-30812557 None:intergenic 45.0%
! CATAGGTGGTCATTGATTGC+TGG + chr4_1:30812628-30812647 Msa0543770:intron 45.0%
ATCTCTTCTGCTGTTCTTCC+AGG - chr4_1:30812661-30812680 None:intergenic 45.0%
GAGAAGAAATAGGGAAGACG+GGG - chr4_1:30811878-30811897 None:intergenic 45.0%
TCAACCTTTGAACCTTGGTC+TGG - chr4_1:30812270-30812289 None:intergenic 45.0%
CTCTAGATCGAAGGTTTGTC+TGG - chr4_1:30812538-30812557 None:intergenic 45.0%
! CATAGGTGGTCATTGATTGC+TGG + chr4_1:30812628-30812647 Msa0543770:intron 45.0%
ATCTCTTCTGCTGTTCTTCC+AGG - chr4_1:30812661-30812680 None:intergenic 45.0%
ACAGATCAGCAACCAGACCA+AGG + chr4_1:30812255-30812274 Msa0543770:intron 50.0%
GCAACCAGACCAAGGTTCAA+AGG + chr4_1:30812263-30812282 Msa0543770:CDS 50.0%
ACACCGACACTCTAGATCGA+AGG - chr4_1:30812547-30812566 None:intergenic 50.0%
ACAGATCAGCAACCAGACCA+AGG + chr4_1:30812255-30812274 Msa0543770:intron 50.0%
GCAACCAGACCAAGGTTCAA+AGG + chr4_1:30812263-30812282 Msa0543770:CDS 50.0%
ACACCGACACTCTAGATCGA+AGG - chr4_1:30812547-30812566 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 30811866 30812751 30811866 ID=Msa0543770;Name=Msa0543770
chr4_1 mRNA 30811866 30812751 30811866 ID=Msa0543770-mRNA-1;Parent=Msa0543770;Name=Msa0543770-mRNA-1;_AED=0.11;_eAED=0.11;_QI=221|1|1|1|1|1|3|31|74
chr4_1 exon 30811866 30812135 30811866 ID=Msa0543770-mRNA-1:exon:8071;Parent=Msa0543770-mRNA-1
chr4_1 exon 30812259 30812346 30812259 ID=Msa0543770-mRNA-1:exon:8072;Parent=Msa0543770-mRNA-1
chr4_1 exon 30812633 30812751 30812633 ID=Msa0543770-mRNA-1:exon:8073;Parent=Msa0543770-mRNA-1
chr4_1 five_prime_UTR 30811866 30812086 30811866 ID=Msa0543770-mRNA-1:five_prime_utr;Parent=Msa0543770-mRNA-1
chr4_1 CDS 30812087 30812135 30812087 ID=Msa0543770-mRNA-1:cds;Parent=Msa0543770-mRNA-1
chr4_1 CDS 30812259 30812346 30812259 ID=Msa0543770-mRNA-1:cds;Parent=Msa0543770-mRNA-1
chr4_1 CDS 30812633 30812720 30812633 ID=Msa0543770-mRNA-1:cds;Parent=Msa0543770-mRNA-1
chr4_1 three_prime_UTR 30812721 30812751 30812721 ID=Msa0543770-mRNA-1:three_prime_utr;Parent=Msa0543770-mRNA-1
Gene Sequence

>Msa0543770

ATGGCTGACTCAGAACACTCATCTAAAAATGTTTCAACAGATTCCAAAGATCAGCAACCAGACCAAGGTTCAAAGGTTGAATTTTCTGAAGAAGAGGAAATGCTTATTAAAATGGTTTATAATCTTGTTGGGGAGAGGTGGTCATTGATTGCTGGAAGAATTCCTGGAAGAACAGCAGAAGAGATAGAAAAATATTGGAATTCAAGACACTCGACTAGCAAATGA

Protein sequence

>Msa0543770

MADSEHSSKNVSTDSKDQQPDQGSKVEFSEEEEMLIKMVYNLVGERWSLIAGRIPGRTAEEIEKYWNSRHSTSK*