AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0544310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0544310 MtrunA17_Chr4g0025061 72.337 629 73 8 1 546 1 611 0.0 800
Msa0544310 MtrunA17_Chr4g0025051 67.818 550 115 7 23 517 25 567 0.0 634
Msa0544310 MtrunA17_Chr4g0024391 69.978 463 108 6 106 546 35 488 0.0 572
Msa0544310 MtrunA17_Chr2g0322391 43.860 513 216 18 44 523 118 591 4.72e-102 320
Msa0544310 MtrunA17_Chr4g0075201 38.330 467 228 15 33 482 41 464 1.99e-75 248
Msa0544310 MtrunA17_Chr2g0288871 36.240 516 228 15 31 495 65 530 9.11e-73 242
Msa0544310 MtrunA17_Chr3g0099641 47.059 238 96 5 263 494 231 444 2.09e-63 215
Msa0544310 MtrunA17_Chr7g0270831 45.850 253 103 6 268 519 201 420 8.76e-57 196
Msa0544310 MtrunA17_Chr2g0302391 48.529 204 90 5 268 459 196 396 1.14e-47 172
Msa0544310 MtrunA17_Chr7g0216891 51.613 124 54 1 240 357 170 293 5.53e-37 145
Msa0544310 MtrunA17_Chr4g0051181 37.453 267 135 6 268 509 228 487 4.64e-36 140
Msa0544310 MtrunA17_Chr4g0060271 44.503 191 75 4 244 403 198 388 6.92e-33 133
Msa0544310 MtrunA17_Chr4g0024401 54.955 111 23 2 16 102 8 115 3.18e-28 109
Msa0544310 MtrunA17_Chr5g0418191 50.515 97 43 2 253 345 286 381 2.25e-24 105
Msa0544310 MtrunA17_Chr5g0418191 37.037 108 64 3 284 391 152 255 4.23e-12 68.6
Msa0544310 MtrunA17_Chr7g0218081 47.959 98 50 1 259 356 386 482 3.12e-24 106
Msa0544310 MtrunA17_Chr3g0101131 44.037 109 57 2 256 361 388 495 4.24e-24 106
Msa0544310 MtrunA17_Chr5g0445311 55.000 80 35 1 266 345 347 425 9.87e-24 105
Msa0544310 MtrunA17_Chr5g0432161 42.222 135 69 3 215 345 353 482 2.05e-23 104
Msa0544310 MtrunA17_Chr5g0432161 48.333 60 29 2 284 343 245 302 8.97e-11 64.7
Msa0544310 MtrunA17_Chr4g0042831 51.852 81 38 1 265 345 260 339 9.16e-23 100
Msa0544310 MtrunA17_Chr4g0001361 35.099 151 74 4 260 410 139 265 9.17e-23 99.4
Msa0544310 MtrunA17_Chr3g0079691 54.321 81 36 1 265 345 477 556 1.17e-22 102
Msa0544310 MtrunA17_Chr3g0079691 46.875 64 32 2 284 347 281 342 9.20e-12 67.8
Msa0544310 MtrunA17_Chr3g0106491 37.415 147 84 4 202 344 283 425 2.30e-22 100
Msa0544310 MtrunA17_Chr3g0106491 40.230 87 46 3 261 347 180 260 4.26e-12 68.6
Msa0544310 MtrunA17_Chr7g0243891 54.321 81 36 1 265 345 356 435 7.59e-22 99.4
Msa0544310 MtrunA17_Chr5g0419241 67.925 53 17 0 261 313 55 107 8.27e-22 90.5
Msa0544310 MtrunA17_Chr4g0004911 53.165 79 36 1 265 343 318 395 1.06e-21 98.6
Msa0544310 MtrunA17_Chr4g0004911 41.860 86 43 3 267 350 170 250 7.22e-12 67.8
Msa0544310 MtrunA17_Chr7g0249301 53.947 76 34 1 270 345 279 353 1.92e-21 96.7
Msa0544310 MtrunA17_Chr7g0249311 50.617 81 39 1 265 345 374 453 3.68e-21 97.8
Msa0544310 MtrunA17_Chr5g0447701 51.807 83 38 2 264 345 232 313 7.00e-21 95.1
Msa0544310 MtrunA17_Chr7g0270191 44.681 94 47 2 268 361 130 218 1.78e-20 92.0
Msa0544310 MtrunA17_Chr1g0150271 36.290 124 64 4 234 343 39 161 1.81e-20 91.3
Msa0544310 MtrunA17_Chr3g0136961 59.322 59 24 0 282 340 137 195 1.82e-20 92.0
Msa0544310 MtrunA17_Chr4g0051881 40.741 108 63 1 270 377 165 271 2.58e-20 92.4
Msa0544310 MtrunA17_Chr2g0333851 48.718 78 38 1 269 344 153 230 5.47e-20 91.3
Msa0544310 MtrunA17_Chr7g0263531 42.857 98 45 2 246 343 334 420 2.25e-19 90.9
Msa0544310 MtrunA17_Chr7g0263531 43.836 73 39 2 284 356 190 260 1.35e-11 67.0
Msa0544310 MtrunA17_Chr1g0150281 43.478 92 52 0 268 359 125 216 2.33e-19 88.6
Msa0544310 MtrunA17_Chr1g0147881 55.224 67 30 0 284 350 263 329 4.27e-19 89.0
Msa0544310 MtrunA17_Chr2g0294231 48.718 78 39 1 270 347 190 266 4.38e-19 89.4
Msa0544310 MtrunA17_Chr1g0150801 51.351 74 35 1 268 341 87 159 5.39e-19 84.3
Msa0544310 MtrunA17_Chr8g0386241 36.697 109 68 1 241 349 61 168 8.46e-19 84.3
Msa0544310 MtrunA17_Chr4g0053281 56.452 62 27 0 284 345 267 328 1.02e-18 87.8
Msa0544310 MtrunA17_Chr7g0224801 47.727 88 38 2 273 360 83 162 1.68e-18 83.2
Msa0544310 MtrunA17_Chr1g0194481 47.436 78 40 1 270 347 145 221 2.63e-18 86.3
Msa0544310 MtrunA17_Chr8g0379461 48.000 75 34 1 283 357 76 145 4.66e-18 84.0
Msa0544310 MtrunA17_Chr5g0401821 56.667 60 26 0 283 342 199 258 4.83e-18 84.3
Msa0544310 MtrunA17_Chr7g0270051 50.746 67 33 0 279 345 49 115 8.95e-18 83.6
Msa0544310 MtrunA17_Chr2g0293481 41.935 93 47 1 268 353 245 337 1.43e-17 85.9
Msa0544310 MtrunA17_Chr5g0443131 47.436 78 40 1 270 347 144 220 1.45e-17 84.0
Msa0544310 MtrunA17_Chr7g0248721 45.263 95 47 3 270 364 128 217 1.75e-17 81.6
Msa0544310 MtrunA17_Chr1g0184691 56.140 57 25 0 284 340 294 350 1.86e-17 84.3
Msa0544310 MtrunA17_Chr4g0074001 32.903 155 79 3 193 347 293 422 2.05e-17 85.5
Msa0544310 MtrunA17_Chr2g0289181 45.455 77 42 0 278 354 9 85 3.10e-17 81.3
Msa0544310 MtrunA17_Chr8g0335811 48.101 79 40 1 262 340 133 210 4.16e-17 80.9
Msa0544310 MtrunA17_Chr2g0319961 34.783 138 74 3 215 352 28 149 5.47e-17 81.3
Msa0544310 MtrunA17_Chr2g0284321 50.820 61 30 0 283 343 35 95 8.69e-17 79.7
Msa0544310 MtrunA17_Chr4g0068541 44.304 79 43 1 269 347 194 271 1.18e-16 82.4
Msa0544310 MtrunA17_Chr3g0129361 42.391 92 51 2 270 361 131 220 1.42e-16 79.0
Msa0544310 MtrunA17_Chr1g0188941 50.000 66 32 1 278 343 46 110 1.61e-16 75.9
Msa0544310 MtrunA17_Chr7g0248691 46.667 75 40 0 279 353 150 224 1.64e-16 80.9
Msa0544310 MtrunA17_Chr8g0383251 34.559 136 82 5 214 346 39 170 1.85e-16 77.8
Msa0544310 MtrunA17_Chr8g0348311 46.667 75 40 0 279 353 151 225 4.82e-16 79.7
Msa0544310 MtrunA17_Chr8g0387501 44.444 72 40 0 282 353 128 199 5.21e-16 78.6
Msa0544310 MtrunA17_Chr4g0071521 53.448 58 27 0 283 340 245 302 6.59e-16 79.0
Msa0544310 MtrunA17_Chr5g0418781 46.154 78 41 1 263 340 104 180 1.17e-15 77.4
Msa0544310 MtrunA17_Chr3g0129321 46.667 75 39 1 270 344 105 178 7.90e-15 73.6
Msa0544310 MtrunA17_Chr8g0383351 38.000 100 60 2 242 340 118 216 7.96e-15 74.3
Msa0544310 MtrunA17_Chr1g0202461 41.935 93 52 1 281 373 259 349 1.74e-14 75.5
Msa0544310 MtrunA17_Chr1g0189391 48.611 72 35 2 270 340 84 154 4.08e-14 73.9
Msa0544310 MtrunA17_Chr6g0485431 47.500 80 40 2 270 348 141 219 6.12e-14 73.6
Msa0544310 MtrunA17_Chr7g0228941 42.029 69 39 1 272 340 42 109 8.93e-14 68.2
Msa0544310 MtrunA17_Chr3g0139021 42.029 69 39 1 272 340 304 371 1.64e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0544310 AT4G04450.1 42.022 445 205 14 77 494 85 503 2.92e-81 264
Msa0544310 AT4G04450.2 41.609 435 201 14 87 494 1 409 7.25e-78 253
Msa0544310 AT1G62300.1 39.827 462 210 18 78 510 130 552 1.73e-68 231
Msa0544310 AT4G22070.4 55.645 248 86 7 262 494 276 514 1.10e-61 213
Msa0544310 AT4G22070.3 55.645 248 86 7 262 494 276 514 1.10e-61 213
Msa0544310 AT4G22070.2 55.645 248 86 7 262 494 276 514 1.10e-61 213
Msa0544310 AT4G22070.1 55.645 248 86 7 262 494 276 514 1.10e-61 213
Msa0544310 AT4G01720.1 43.581 296 124 10 217 491 170 443 1.00e-46 171
Msa0544310 AT5G15130.2 36.531 271 121 7 263 495 165 422 1.44e-38 149
Msa0544310 AT5G15130.1 36.531 271 121 7 263 495 207 464 1.51e-38 149
Msa0544310 AT1G68150.1 62.887 97 36 0 268 364 220 316 2.78e-38 145
Msa0544310 AT1G69810.2 44.318 176 87 5 237 411 103 268 3.60e-35 135
Msa0544310 AT1G69810.1 44.318 176 87 5 237 411 161 326 5.10e-35 136
Msa0544310 AT1G18860.1 46.061 165 80 3 266 421 222 386 1.17e-33 135
Msa0544310 AT2G03340.1 44.828 116 54 2 230 345 371 476 3.48e-24 106
Msa0544310 AT2G03340.1 51.667 60 27 2 284 343 251 308 1.33e-11 67.4
Msa0544310 AT1G13960.2 55.696 79 34 1 265 343 238 315 5.55e-24 103
Msa0544310 AT1G13960.2 47.222 72 36 2 284 355 77 146 8.56e-12 67.4
Msa0544310 AT1G13960.1 55.696 79 34 1 265 343 391 468 6.74e-24 105
Msa0544310 AT1G13960.1 47.222 72 36 2 284 355 230 299 6.79e-12 68.6
Msa0544310 AT4G26640.5 52.500 80 37 1 266 345 188 266 7.54e-24 103
Msa0544310 AT4G26640.6 52.500 80 37 1 266 345 188 266 7.54e-24 103
Msa0544310 AT4G26640.4 52.500 80 37 1 266 345 188 266 7.54e-24 103
Msa0544310 AT4G26640.3 52.500 80 37 1 266 345 188 266 7.54e-24 103
Msa0544310 AT2G38470.1 54.321 81 36 1 265 345 344 423 1.35e-23 105
Msa0544310 AT2G38470.1 38.462 91 54 2 278 368 179 267 5.40e-11 65.5
Msa0544310 AT4G26640.7 52.500 80 37 1 266 345 324 402 1.99e-23 104
Msa0544310 AT4G26640.1 52.500 80 37 1 266 345 292 370 2.03e-23 103
Msa0544310 AT4G26640.2 52.500 80 37 1 266 345 364 442 2.52e-23 104
Msa0544310 AT2G04880.2 45.055 91 49 1 253 343 253 342 7.61e-22 99.0
Msa0544310 AT2G04880.2 49.275 69 31 3 279 346 107 172 6.04e-12 68.6
Msa0544310 AT5G56270.1 53.086 81 37 1 265 345 469 548 8.50e-22 100
Msa0544310 AT2G04880.1 45.055 91 49 1 253 343 277 366 1.10e-21 99.0
Msa0544310 AT2G04880.1 49.275 69 31 3 279 346 107 172 6.73e-12 68.6
Msa0544310 AT3G01080.2 48.837 86 43 1 258 343 319 403 1.48e-21 98.2
Msa0544310 AT3G01080.1 48.837 86 43 1 258 343 281 365 1.72e-21 97.8
Msa0544310 AT4G31800.2 43.810 105 54 1 262 361 85 189 1.98e-21 94.4
Msa0544310 AT4G31800.1 43.810 105 54 1 262 361 150 254 2.91e-21 95.1
Msa0544310 AT5G07100.2 48.235 85 43 1 263 347 121 204 6.36e-21 92.0
Msa0544310 AT5G07100.4 48.235 85 43 1 263 347 121 204 6.36e-21 92.0
Msa0544310 AT5G07100.5 48.235 85 43 1 263 347 121 204 6.36e-21 92.0
Msa0544310 AT5G07100.3 48.235 85 43 1 263 347 121 204 6.36e-21 92.0
Msa0544310 AT5G07100.1 48.235 85 43 1 263 347 214 297 1.18e-20 93.2
Msa0544310 AT1G80840.1 31.915 141 88 2 210 344 69 207 1.25e-20 93.2
Msa0544310 AT5G41570.1 45.455 99 49 2 270 368 85 178 1.52e-20 89.7
Msa0544310 AT1G29860.1 50.000 80 39 1 268 347 121 199 2.53e-20 91.7
Msa0544310 AT1G64000.1 45.833 96 47 2 265 360 96 186 3.07e-20 89.4
Msa0544310 AT2G25000.3 40.385 104 62 0 243 346 85 188 5.25e-20 90.1
Msa0544310 AT2G25000.2 40.385 104 62 0 243 346 63 166 5.55e-20 89.7
Msa0544310 AT2G25000.4 40.385 104 62 0 243 346 63 166 5.55e-20 89.7
Msa0544310 AT4G18170.1 43.000 100 56 1 270 369 159 257 5.76e-20 91.7
Msa0544310 AT2G25000.1 40.385 104 62 0 243 346 106 209 9.52e-20 89.7
Msa0544310 AT5G26170.1 52.778 72 33 1 269 340 99 169 1.03e-19 87.0
Msa0544310 AT2G30250.1 46.512 86 45 1 262 347 307 391 1.90e-19 91.3
Msa0544310 AT5G46350.1 52.703 74 34 1 270 343 170 242 3.36e-19 89.4
Msa0544310 AT2G23320.1 59.322 59 24 0 284 342 241 299 4.57e-19 89.0
Msa0544310 AT5G49520.1 46.250 80 42 1 268 347 206 284 4.91e-19 90.1
Msa0544310 AT2G30590.1 57.812 64 26 1 284 346 314 377 5.78e-19 89.4
Msa0544310 AT1G29280.1 50.000 70 35 0 274 343 65 134 7.19e-19 87.0
Msa0544310 AT4G30935.1 38.710 124 74 2 251 373 299 421 7.69e-19 90.1
Msa0544310 AT5G13080.1 52.055 73 34 1 270 342 54 125 1.36e-18 83.2
Msa0544310 AT4G24240.1 56.667 60 26 0 283 342 281 340 2.55e-18 87.0
Msa0544310 AT1G55600.1 48.684 76 38 1 270 345 294 368 2.76e-18 88.6
Msa0544310 AT4G26440.1 51.316 76 36 1 270 345 359 433 3.14e-18 88.6
Msa0544310 AT5G28650.1 60.377 53 21 0 288 340 267 319 1.55e-17 84.3
Msa0544310 AT3G58710.2 46.575 73 39 0 274 346 61 133 1.62e-17 83.2
Msa0544310 AT3G58710.1 46.575 73 39 0 274 346 62 134 1.63e-17 83.6
Msa0544310 AT5G64810.1 46.809 94 46 2 250 340 74 166 1.91e-17 81.3
Msa0544310 AT3G01970.1 51.389 72 34 1 269 340 51 121 2.19e-17 79.7
Msa0544310 AT2G44745.1 39.286 112 63 3 232 340 92 201 2.40e-17 81.6
Msa0544310 AT3G62340.1 52.703 74 34 1 270 343 105 177 3.33e-17 82.4
Msa0544310 AT3G04670.1 57.895 57 24 0 284 340 263 319 3.34e-17 83.6
Msa0544310 AT2G47260.1 45.783 83 44 1 268 350 159 240 3.65e-17 83.6
Msa0544310 AT4G01250.1 46.429 84 43 1 270 353 117 198 4.06e-17 82.8
Msa0544310 AT4G31550.1 57.895 57 24 0 284 340 247 303 4.39e-17 83.2
Msa0544310 AT4G31550.2 57.895 57 24 0 284 340 246 302 4.46e-17 83.2
Msa0544310 AT2G24570.1 57.895 57 24 0 284 340 244 300 5.73e-17 82.8
Msa0544310 AT5G52830.1 45.455 77 42 0 277 353 159 235 5.73e-17 83.2
Msa0544310 AT2G46130.1 47.619 84 42 2 259 341 5 87 8.20e-17 77.0
Msa0544310 AT2G34830.1 43.011 93 46 1 265 350 190 282 8.99e-17 83.2
Msa0544310 AT2G34830.2 43.011 93 46 1 265 350 199 291 1.08e-16 83.2
Msa0544310 AT1G30650.1 43.011 93 46 1 265 350 192 284 1.09e-16 83.2
Msa0544310 AT4G23550.1 44.872 78 43 0 278 355 135 212 2.99e-16 80.5
Msa0544310 AT5G45050.2 26.337 243 160 6 211 446 1082 1312 8.41e-16 81.6
Msa0544310 AT5G45050.1 26.337 243 160 6 211 446 1110 1340 8.69e-16 81.6
Msa0544310 AT5G45050.4 26.337 243 160 6 211 446 971 1201 8.77e-16 81.6
Msa0544310 AT4G39410.1 50.704 71 34 1 270 340 210 279 1.30e-15 78.2
Msa0544310 AT2G37260.2 53.846 65 29 1 278 342 262 325 2.48e-15 78.2
Msa0544310 AT2G37260.2 48.529 68 28 3 284 346 84 149 1.25e-11 66.6
Msa0544310 AT1G69310.4 44.000 75 41 1 266 340 130 203 2.54e-15 77.0
Msa0544310 AT1G69310.2 44.000 75 41 1 266 340 130 203 2.54e-15 77.0
Msa0544310 AT1G69310.1 44.000 75 41 1 266 340 130 203 2.54e-15 77.0
Msa0544310 AT2G37260.3 53.846 65 29 1 278 342 264 327 2.55e-15 78.2
Msa0544310 AT2G37260.3 48.529 68 28 3 284 346 86 151 1.22e-11 67.0
Msa0544310 AT2G37260.1 53.846 65 29 1 278 342 344 407 3.38e-15 78.6
Msa0544310 AT2G37260.1 48.529 68 28 3 284 346 166 231 1.25e-11 67.4
Msa0544310 AT2G46130.2 45.238 84 40 3 259 341 5 83 4.99e-13 65.9
Msa0544310 AT2G21900.1 42.029 69 39 1 278 346 104 171 5.34e-13 68.6
Msa0544310 AT5G45260.1 31.847 157 87 4 213 357 1136 1284 4.92e-12 69.7
Msa0544310 AT4G31800.3 48.438 64 28 1 262 320 150 213 7.17e-12 66.2

Find 93 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGTTACACCATCTTTAT+TGG 0.095497 4_1:-32558806 Msa0544310:CDS
CCAAAACTCTGAGGGATTAA+AGG 0.176833 4_1:+32555442 None:intergenic
GACAAAGCTGCAGGAATATT+TGG 0.214770 4_1:+32555265 None:intergenic
GTTCTCTTCTCTTCTGAATT+AGG 0.215204 4_1:+32558538 None:intergenic
GCTGCTATTACATCCATTAT+TGG 0.245842 4_1:-32555241 Msa0544310:CDS
TAAAGTTTAGGTCTTGTAAT+TGG 0.259816 4_1:+32555050 None:intergenic
CCTTTAATCCCTCAGAGTTT+TGG 0.262834 4_1:-32555442 Msa0544310:CDS
GGGAAAGAGTTGTTATTAAC+TGG 0.286280 4_1:+32559038 None:intergenic
ATTGTTTGAGCAGTAATATT+TGG 0.291797 4_1:+32555310 None:intergenic
GCGTTCCTTTGTGGAGTATT+TGG 0.301041 4_1:+32555481 None:intergenic
CGTAAGGGTAAGAAAGTTAA+AGG 0.311737 4_1:-32557187 Msa0544310:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.318235 4_1:-32556947 Msa0544310:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.335137 4_1:-32557067 Msa0544310:intron
AGCTGACCCTTCACAAATAT+TGG 0.335185 4_1:-32555338 Msa0544310:CDS
AGAAGAAACTTACATGCTTT+CGG 0.356423 4_1:+32556413 None:intergenic
GGATGTCAATGGAGAAAGTA+TGG 0.356478 4_1:-32556508 Msa0544310:CDS
CTACTAGTATTGATAGTAAT+AGG 0.368547 4_1:+32558592 None:intergenic
AGAGAAGAGAACTAAGAGTA+TGG 0.375742 4_1:-32558527 Msa0544310:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.381386 4_1:+32556457 None:intergenic
AACTGATTTGCGTTCCTTTG+TGG 0.385254 4_1:+32555472 None:intergenic
AAAAGGAAATTTGATGATGC+TGG 0.391734 4_1:-32556980 Msa0544310:CDS
GCTGCTGATAATAATAATAA+TGG 0.393320 4_1:-32555190 Msa0544310:CDS
GCCATTTCTTTAGCTGCTGC+AGG 0.395885 4_1:+32555676 None:intergenic
TCAATTGTAATAGTTGGATA+TGG 0.399033 4_1:+32555517 None:intergenic
CTTTAGCTGCTGCAGGTAAA+GGG 0.402829 4_1:+32555683 None:intergenic
TCTTTAGCTGCTGCAGGTAA+AGG 0.405534 4_1:+32555682 None:intergenic
TAACATCCTCATTCTTACTT+AGG 0.406214 4_1:+32556711 None:intergenic
GCAGCAGCAGTGTTGTTGTT+TGG 0.408470 4_1:+32555208 None:intergenic
CTTTAACTTTCTTACCCTTA+CGG 0.409546 4_1:+32557188 None:intergenic
GCTACCATTGCTATTTCCTT+CGG 0.410299 4_1:+32555156 None:intergenic
TCAAGGTTGTTAGTATCATC+AGG 0.414586 4_1:+32559017 None:intergenic
TGATGGGAAGGAACGAAATC+AGG 0.420611 4_1:-32555077 Msa0544310:CDS
GTGTAATCAATTGTAATAGT+TGG 0.425606 4_1:+32555511 None:intergenic
AACTTACATGCTTTCGGACT+GGG 0.430854 4_1:+32556419 None:intergenic
AAAATGAGAATTAAGAATGA+TGG 0.434196 4_1:-32557013 Msa0544310:CDS
TGGCTGAGTATCACCAATAA+TGG 0.440113 4_1:+32555228 None:intergenic
GTATGAATATGATCATTGTC+AGG 0.450321 4_1:+32558826 None:intergenic
ACCTGCAGCAGCTAAAGAAA+TGG 0.455287 4_1:-32555677 Msa0544310:CDS
ATTGGCTACTGATAAGGTTA+AGG 0.459989 4_1:-32556957 Msa0544310:CDS
AGCTTTAGTTCCAATAAAGA+TGG 0.463299 4_1:+32558796 None:intergenic
ACATGTGGCATCAACAATGA+TGG 0.482281 4_1:-32555094 Msa0544310:CDS
GAAATTTGATGATGCTGGAT+TGG 0.483257 4_1:-32556975 Msa0544310:CDS
ATATGATCATTGTCAGGGAC+AGG 0.484701 4_1:+32558832 None:intergenic
GCATCAACATCAAGCAATGA+TGG 0.494138 4_1:-32555616 Msa0544310:CDS
TCTAGAAGTCAAGATCAGTT+AGG 0.495524 4_1:-32556776 Msa0544310:CDS
TGCTGGATTGGCTACTGATA+AGG 0.503894 4_1:-32556963 Msa0544310:CDS
GCTTTCGGACTGGGCAACCA+TGG 0.504496 4_1:+32556428 None:intergenic
ATGATGGAGAATTAATGAAA+AGG 0.504955 4_1:-32556997 Msa0544310:CDS
AAACTTACATGCTTTCGGAC+TGG 0.512195 4_1:+32556418 None:intergenic
GAGAGTGATGCAAGACCGTA+AGG 0.513027 4_1:-32557203 Msa0544310:CDS
GGTATTGCCAATATTTGTGA+AGG 0.514432 4_1:+32555331 None:intergenic
TAGTAGTGATCAATCTATGA+TGG 0.520634 4_1:-32558575 Msa0544310:CDS
CGATAATAAGCTTTAGGACA+CGG 0.531100 4_1:+32556463 None:intergenic
GTTGTAATCACAACTTATGA+AGG 0.531127 4_1:-32555718 Msa0544310:CDS
TTATTGAGTTAGATCACTGA+TGG 0.542017 4_1:-32556529 Msa0544310:intron
TGGGACTAAGTTTCAACTCA+AGG 0.543159 4_1:+32559000 None:intergenic
CTTTCGGACTGGGCAACCAT+GGG 0.546480 4_1:+32556429 None:intergenic
AGGAATTTCCAAAACTCTGA+GGG 0.547821 4_1:+32555434 None:intergenic
ACTTCAGAATGGTGGAGAGT+TGG 0.548067 4_1:-32556885 Msa0544310:intron
AGTATTGATAGTAATAGGAA+TGG 0.556477 4_1:+32558597 None:intergenic
AAAGGAAAAGAAGTGTTCAC+TGG 0.557236 4_1:-32556929 Msa0544310:CDS
GTATTGATAGTAATAGGAAT+GGG 0.563883 4_1:+32558598 None:intergenic
TATCGCTGCAGCACGACCCA+TGG 0.564450 4_1:-32556445 Msa0544310:CDS
ATAGCAGCTGACAAAGCTGC+AGG 0.574982 4_1:+32555256 None:intergenic
GAAGAGAACTAAGAGTATGG+AGG 0.575623 4_1:-32558524 Msa0544310:intron
GATAATAAGCTTTAGGACAC+GGG 0.575823 4_1:+32556464 None:intergenic
GATCAATCTATGATGGATGA+TGG 0.577380 4_1:-32558568 Msa0544310:CDS
AGATCACTGATGGATGTCAA+TGG 0.579642 4_1:-32556519 Msa0544310:CDS
AGCAACAAAATTATCACATG+TGG 0.580366 4_1:-32555109 Msa0544310:CDS
TAGCTGCTGCAGGTAAAGGG+TGG 0.582781 4_1:+32555686 None:intergenic
GTATTGCCAATATTTGTGAA+GGG 0.582929 4_1:+32555332 None:intergenic
CATGTGGCATCAACAATGAT+GGG 0.583795 4_1:-32555093 Msa0544310:CDS
TACAAATGCTGATATTGTGA+TGG 0.589604 4_1:-32556831 Msa0544310:CDS
GATAGTCCTAAGTAAGAATG+AGG 0.591251 4_1:-32556717 Msa0544310:CDS
TGGCATCAACAATGATGGGA+AGG 0.604261 4_1:-32555089 Msa0544310:CDS
ATTAACTGGAACTGAAGCCA+TGG 0.605747 4_1:+32559052 None:intergenic
TTGAGGGATCAGAGAAGCTG+AGG 0.609486 4_1:+32555414 None:intergenic
AATATTTGGACTAGCAGCAA+TGG 0.612014 4_1:+32555279 None:intergenic
TATGAATATGATCATTGTCA+GGG 0.613441 4_1:+32558827 None:intergenic
GATGTCAATGGAGAAAGTAT+GGG 0.615340 4_1:-32556507 Msa0544310:CDS
TTAAAACTTAACAGGTACAA+AGG 0.618245 4_1:-32555759 Msa0544310:intron
TGTTAATGTTGATAATGTTG+AGG 0.619880 4_1:-32556693 Msa0544310:CDS
AAGTCAAGATCAGTTAGGAT+CGG 0.620379 4_1:-32556771 Msa0544310:CDS
TATGATCATTGTCAGGGACA+GGG 0.624825 4_1:+32558833 None:intergenic
CAAGGTTGTTAGTATCATCA+GGG 0.625070 4_1:+32559018 None:intergenic
GTCACCGAAGGAAATAGCAA+TGG 0.626521 4_1:-32555160 Msa0544310:CDS
AATGGCAACAATGTCACCGA+AGG 0.626879 4_1:-32555172 Msa0544310:CDS
GAGGAATTTCCAAAACTCTG+AGG 0.632072 4_1:+32555433 None:intergenic
AGCTAGATCAGAAGAAACCA+TGG 0.635656 4_1:-32556639 Msa0544310:intron
GGACAGAGACAACTTCATGA+TGG 0.651443 4_1:+32558854 None:intergenic
AATCTCCAAATACTCCACAA+AGG 0.678231 4_1:-32555486 Msa0544310:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4_1:+32555556 None:intergenic
AGAGTGATGCAAGACCGTAA+GGG 0.707223 4_1:-32557202 Msa0544310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTCTCAAATTATTAT+TGG - chr4_1:32556426-32556445 Msa0544310:CDS 10.0%
!! AAATATAATCACATAAAAAA+CGG + chr4_1:32556722-32556741 None:intergenic 10.0%
!!! ATTAATTCTTTTTAGTAAAA+AGG + chr4_1:32557526-32557545 None:intergenic 10.0%
!! TAATTAAATTAATTATGAAG+TGG - chr4_1:32558266-32558285 Msa0544310:intron 10.0%
!! CGTAAAAAAAAAATAACAAT+GGG + chr4_1:32555333-32555352 None:intergenic 15.0%
!! ACGTAAAAAAAAAATAACAA+TGG + chr4_1:32555334-32555353 None:intergenic 15.0%
!!! TTTTGACTCAATAAAATTAA+AGG + chr4_1:32555643-32555662 None:intergenic 15.0%
!! TGTATTATACTTATAAATGT+AGG + chr4_1:32555960-32555979 None:intergenic 15.0%
!! TATAACATAATTATTAGTTG+TGG + chr4_1:32556036-32556055 None:intergenic 15.0%
!! TTTAGTAAATACATGTAATT+TGG + chr4_1:32557698-32557717 None:intergenic 15.0%
!! TAATACAATTAAAACTTAAC+AGG - chr4_1:32558310-32558329 Msa0544310:intron 15.0%
!! AGCAAAAAAAATTAAAGTTT+AGG + chr4_1:32559042-32559061 None:intergenic 15.0%
!! TAATTTCATTAAATTCTGCT+AGG + chr4_1:32555599-32555618 None:intergenic 20.0%
!!! TAATTTTATTGAGTCAAAAC+TGG - chr4_1:32555644-32555663 Msa0544310:CDS 20.0%
!!! AATTTTATTGAGTCAAAACT+GGG - chr4_1:32555645-32555664 Msa0544310:CDS 20.0%
!! AAAAAATTTAAAAGAAAGCG+AGG + chr4_1:32555775-32555794 None:intergenic 20.0%
!!! TTCTACTATGTTATTCTTTT+TGG - chr4_1:32555793-32555812 Msa0544310:intron 20.0%
!! ATATATATATGTGTGATTCT+TGG - chr4_1:32555893-32555912 Msa0544310:intron 20.0%
!!! TAATTTTTTTCACAAAGAAG+AGG + chr4_1:32556531-32556550 None:intergenic 20.0%
!! GAATATATTACAATTACATG+CGG - chr4_1:32556840-32556859 Msa0544310:CDS 20.0%
!! AAAATGAGAATTAAGAATGA+TGG - chr4_1:32557064-32557083 Msa0544310:CDS 20.0%
!!! ACATGCATATTTTAAAACAT+TGG - chr4_1:32557873-32557892 Msa0544310:intron 20.0%
!!! AATTTTGATTGGTTTTGATT+AGG + chr4_1:32558692-32558711 None:intergenic 20.0%
! TATGAATATGATCATTGTCA+GGG + chr4_1:32555253-32555272 None:intergenic 25.0%
! AACAATGGGATATTGAATTA+TGG + chr4_1:32555319-32555338 None:intergenic 25.0%
!! GTATTGATAGTAATAGGAAT+GGG + chr4_1:32555482-32555501 None:intergenic 25.0%
!!! AGTATTGATAGTAATAGGAA+TGG + chr4_1:32555483-32555502 None:intergenic 25.0%
!!! CTACTAGTATTGATAGTAAT+AGG + chr4_1:32555488-32555507 None:intergenic 25.0%
!!! CTATGTTATTCTTTTTGGAA+AGG - chr4_1:32555798-32555817 Msa0544310:intron 25.0%
! GTCATATTCATTAATTAACG+TGG + chr4_1:32556128-32556147 None:intergenic 25.0%
! ATTTAATTAGCGTACTATGT+CGG + chr4_1:32556224-32556243 None:intergenic 25.0%
! AAAATGTAACAGTTTATGTC+AGG + chr4_1:32556400-32556419 None:intergenic 25.0%
! CACTGACATACATATAAAAT+CGG + chr4_1:32556481-32556500 None:intergenic 25.0%
!! CATGTTAATAGTGTTCTAAA+AGG + chr4_1:32556592-32556611 None:intergenic 25.0%
! TTAGAACACTATTAACATGA+TGG - chr4_1:32556593-32556612 Msa0544310:intron 25.0%
! TAGAACACTATTAACATGAT+GGG - chr4_1:32556594-32556613 Msa0544310:intron 25.0%
! CCAACAAAATCAAAATACAT+TGG + chr4_1:32556622-32556641 None:intergenic 25.0%
!!! CCAATGTATTTTGATTTTGT+TGG - chr4_1:32556619-32556638 Msa0544310:intron 25.0%
!! AATCACATAAAAAACGGATT+TGG + chr4_1:32556716-32556735 None:intergenic 25.0%
!! TTCATTGAACTATTTTCAGA+TGG - chr4_1:32556745-32556764 Msa0544310:CDS 25.0%
! AAAGGAAAAAAAGTCTTTGA+TGG - chr4_1:32556908-32556927 Msa0544310:CDS 25.0%
!! ATGATGGAGAATTAATGAAA+AGG - chr4_1:32557080-32557099 Msa0544310:intron 25.0%
!!! TGTTAATGTTGATAATGTTG+AGG - chr4_1:32557384-32557403 Msa0544310:intron 25.0%
!! ACTAAAAATGTTTCTGTATG+TGG + chr4_1:32557948-32557967 None:intergenic 25.0%
!! TATGTTTCTATGCTAGAAAA+TGG - chr4_1:32558047-32558066 Msa0544310:intron 25.0%
! TAAATACATAGATTGCTGAA+TGG + chr4_1:32558086-32558105 None:intergenic 25.0%
! TCAAAATATGCAGTTATGTT+TGG - chr4_1:32558136-32558155 Msa0544310:intron 25.0%
! CAATAAACTCAAATCCAAAA+TGG - chr4_1:32558226-32558245 Msa0544310:intron 25.0%
! TTAAAACTTAACAGGTACAA+AGG - chr4_1:32558318-32558337 Msa0544310:intron 25.0%
! TCAATTGTAATAGTTGGATA+TGG + chr4_1:32558563-32558582 None:intergenic 25.0%
! GTGTAATCAATTGTAATAGT+TGG + chr4_1:32558569-32558588 None:intergenic 25.0%
!! CAAAACCAATCAAAATTTTC+TGG - chr4_1:32558695-32558714 Msa0544310:intron 25.0%
! ATTGTTTGAGCAGTAATATT+TGG + chr4_1:32558770-32558789 None:intergenic 25.0%
! GCTGCTGATAATAATAATAA+TGG - chr4_1:32558887-32558906 Msa0544310:CDS 25.0%
!! TGTGATAATTTTGTTGCTAT+TGG + chr4_1:32558966-32558985 None:intergenic 25.0%
!! TAAAGTTTAGGTCTTGTAAT+TGG + chr4_1:32559030-32559049 None:intergenic 25.0%
! TTTTCATTGGAACTAGGAAA+GGG + chr4_1:32555134-32555153 None:intergenic 30.0%
! ATTTTCATTGGAACTAGGAA+AGG + chr4_1:32555135-32555154 None:intergenic 30.0%
!! GTTTGATTTTCATTGGAACT+AGG + chr4_1:32555140-32555159 None:intergenic 30.0%
GTATGAATATGATCATTGTC+AGG + chr4_1:32555254-32555273 None:intergenic 30.0%
TACTGTTACACCATCTTTAT+TGG - chr4_1:32555271-32555290 Msa0544310:CDS 30.0%
! AGCTTTAGTTCCAATAAAGA+TGG + chr4_1:32555284-32555303 None:intergenic 30.0%
TAGTAGTGATCAATCTATGA+TGG - chr4_1:32555502-32555521 Msa0544310:CDS 30.0%
ATATATGTGTGATTCTTGGT+TGG - chr4_1:32555897-32555916 Msa0544310:intron 30.0%
!! CCTGAAAATTTGAGTATTGA+GGG + chr4_1:32556354-32556373 None:intergenic 30.0%
! CCTCAATACTCAAATTTTCA+GGG - chr4_1:32556352-32556371 Msa0544310:intron 30.0%
AGTTTATGTCAGGATCATAT+TGG + chr4_1:32556390-32556409 None:intergenic 30.0%
AGAACACTATTAACATGATG+GGG - chr4_1:32556595-32556614 Msa0544310:intron 30.0%
CGTTCGAATAAAACATGTTA+GGG + chr4_1:32556684-32556703 None:intergenic 30.0%
ACGTTCGAATAAAACATGTT+AGG + chr4_1:32556685-32556704 None:intergenic 30.0%
! CTTTAACTTTCTTACCCTTA+CGG + chr4_1:32556892-32556911 None:intergenic 30.0%
GAAATTTATGGAACTTGGAT+TGG - chr4_1:32556982-32557001 Msa0544310:CDS 30.0%
GATAAGGTTAAGGAAGTTAA+AGG - chr4_1:32557010-32557029 Msa0544310:CDS 30.0%
!! AAAAGGAAATTTGATGATGC+TGG - chr4_1:32557097-32557116 Msa0544310:intron 30.0%
GATAAGGTTAAGGAAGTTAA+AGG - chr4_1:32557010-32557029 Msa0544310:CDS 30.0%
AAGAAAAAGAGACTTCAGAA+TGG - chr4_1:32557181-32557200 Msa0544310:CDS 30.0%
TACAAATGCTGATATTGTGA+TGG - chr4_1:32557246-32557265 Msa0544310:intron 30.0%
TAACATCCTCATTCTTACTT+AGG + chr4_1:32557369-32557388 None:intergenic 30.0%
! ACACTTTTACATACTTACCA+TGG + chr4_1:32557458-32557477 None:intergenic 30.0%
TTATTGAGTTAGATCACTGA+TGG - chr4_1:32557548-32557567 Msa0544310:intron 30.0%
AGAAGAAACTTACATGCTTT+CGG + chr4_1:32557667-32557686 None:intergenic 30.0%
TAATGCGAAAGCAATCAATA+GGG + chr4_1:32557738-32557757 None:intergenic 30.0%
ATAATGCGAAAGCAATCAAT+AGG + chr4_1:32557739-32557758 None:intergenic 30.0%
!! AGAAACATAAGCTTTGAAAG+TGG + chr4_1:32558036-32558055 None:intergenic 30.0%
!!! CTGCATATTTTGATTTGCAA+TGG + chr4_1:32558129-32558148 None:intergenic 30.0%
GTTGTAATCACAACTTATGA+AGG - chr4_1:32558359-32558378 Msa0544310:intron 30.0%
!!! GGTTTTGATTAGGTATTTGA+GGG + chr4_1:32558682-32558701 None:intergenic 30.0%
!!! TGGTTTTGATTAGGTATTTG+AGG + chr4_1:32558683-32558702 None:intergenic 30.0%
!! GTAGTCCAGAAAATTTTGAT+TGG + chr4_1:32558703-32558722 None:intergenic 30.0%
GTATTGCCAATATTTGTGAA+GGG + chr4_1:32558748-32558767 None:intergenic 30.0%
AGCAACAAAATTATCACATG+TGG - chr4_1:32558968-32558987 Msa0544310:intron 30.0%
! GGGAAAGAGTTGTTATTAAC+TGG + chr4_1:32555042-32555061 None:intergenic 35.0%
CAAGGTTGTTAGTATCATCA+GGG + chr4_1:32555062-32555081 None:intergenic 35.0%
TCAAGGTTGTTAGTATCATC+AGG + chr4_1:32555063-32555082 None:intergenic 35.0%
ACTTAGTCCCAACAAAAACA+TGG - chr4_1:32555089-32555108 Msa0544310:CDS 35.0%
!! GTAGCATCCATGTTTTTGTT+GGG + chr4_1:32555099-32555118 None:intergenic 35.0%
!! TGTAGCATCCATGTTTTTGT+TGG + chr4_1:32555100-32555119 None:intergenic 35.0%
!! TGGAGAGGTTTGATTTTCAT+TGG + chr4_1:32555147-32555166 None:intergenic 35.0%
CTTGTTTCATGAGATGAGAT+TGG + chr4_1:32555167-32555186 None:intergenic 35.0%
TCATGAAACAAGAACAGAGT+TGG - chr4_1:32555175-32555194 Msa0544310:CDS 35.0%
!!! TTCTTTTTTCGCATACAGCT+TGG - chr4_1:32555455-32555474 Msa0544310:CDS 35.0%
!! GATCAATCTATGATGGATGA+TGG - chr4_1:32555509-32555528 Msa0544310:CDS 35.0%
GTTCTCTTCTCTTCTGAATT+AGG + chr4_1:32555542-32555561 None:intergenic 35.0%
AGAGAAGAGAACTAAGAGTA+TGG - chr4_1:32555550-32555569 Msa0544310:CDS 35.0%
TCGACACTCTTTAGATTTGA+AGG - chr4_1:32555685-32555704 Msa0544310:CDS 35.0%
ACCCACGTACACAAAAATAT+GGG - chr4_1:32555742-32555761 Msa0544310:CDS 35.0%
TTAACGTGGTAGCATATCAT+TGG + chr4_1:32556114-32556133 None:intergenic 35.0%
! CCCTCAATACTCAAATTTTC+AGG - chr4_1:32556351-32556370 Msa0544310:intron 35.0%
! CCCTGAAAATTTGAGTATTG+AGG + chr4_1:32556355-32556374 None:intergenic 35.0%
GATCTCACTATTACAATGCA+TGG - chr4_1:32556566-32556585 Msa0544310:intron 35.0%
!! ATGTTTTATTCGAACGTGAG+AGG - chr4_1:32556688-32556707 Msa0544310:CDS 35.0%
TTTCAGATGGCTATTCTTCA+AGG - chr4_1:32556758-32556777 Msa0544310:CDS 35.0%
AGAGCTTGAACAAACGAAAA+TGG - chr4_1:32556781-32556800 Msa0544310:CDS 35.0%
CGTAAGGGTAAGAAAGTTAA+AGG - chr4_1:32556890-32556909 Msa0544310:CDS 35.0%
GAGAAAAAGAGAACTGAGAA+TGG - chr4_1:32556941-32556960 Msa0544310:CDS 35.0%
AAAAAGAGAACTGAGAATGG+TGG - chr4_1:32556944-32556963 Msa0544310:CDS 35.0%
CCAAGTTCCATAAATTTCCT+TGG + chr4_1:32556980-32556999 None:intergenic 35.0%
CCAAGGAAATTTATGGAACT+TGG - chr4_1:32556977-32556996 Msa0544310:CDS 35.0%
ATTGGCTACTGATAAGGTTA+AGG - chr4_1:32557000-32557019 Msa0544310:CDS 35.0%
!! GAAATTTGATGATGCTGGAT+TGG - chr4_1:32557102-32557121 Msa0544310:intron 35.0%
ATTGGCTACTGATAAGGTTA+AGG - chr4_1:32557000-32557019 Msa0544310:CDS 35.0%
!! AAAGGAAAAGAAGTGTTCAC+TGG - chr4_1:32557148-32557167 Msa0544310:CDS 35.0%
AAAAAGAGACTTCAGAATGG+TGG - chr4_1:32557184-32557203 Msa0544310:CDS 35.0%
! CTAGATTTTCTCATTGACGA+GGG + chr4_1:32557286-32557305 None:intergenic 35.0%
! TCTAGATTTTCTCATTGACG+AGG + chr4_1:32557287-32557306 None:intergenic 35.0%
TCTAGAAGTCAAGATCAGTT+AGG - chr4_1:32557301-32557320 Msa0544310:intron 35.0%
AAGTCAAGATCAGTTAGGAT+CGG - chr4_1:32557306-32557325 Msa0544310:intron 35.0%
GATAGTCCTAAGTAAGAATG+AGG - chr4_1:32557360-32557379 Msa0544310:intron 35.0%
! GATGTCAATGGAGAAAGTAT+GGG - chr4_1:32557570-32557589 Msa0544310:intron 35.0%
TATGGGCAAAAACTATCGAA+AGG - chr4_1:32557587-32557606 Msa0544310:intron 35.0%
!! GATAATAAGCTTTAGGACAC+GGG + chr4_1:32557616-32557635 None:intergenic 35.0%
!! CGATAATAAGCTTTAGGACA+CGG + chr4_1:32557617-32557636 None:intergenic 35.0%
CATGTTTGTGACTATGATCT+AGG + chr4_1:32557858-32557877 None:intergenic 35.0%
TCACGTAATAAGCACATTGA+TGG + chr4_1:32558186-32558205 None:intergenic 35.0%
TTATTGCATTCAGCAAATCC+AGG + chr4_1:32558212-32558231 None:intergenic 35.0%
! TAGCTAAGTAGCTACCATTT+TGG + chr4_1:32558243-32558262 None:intergenic 35.0%
AAAATGGTAGCTACTTAGCT+AGG - chr4_1:32558242-32558261 Msa0544310:intron 35.0%
GTTGCAATACTTGAAGAACA+AGG + chr4_1:32558524-32558543 None:intergenic 35.0%
AATCTCCAAATACTCCACAA+AGG - chr4_1:32558591-32558610 Msa0544310:CDS 35.0%
AGGAATTTCCAAAACTCTGA+GGG + chr4_1:32558646-32558665 None:intergenic 35.0%
GGTATTGCCAATATTTGTGA+AGG + chr4_1:32558749-32558768 None:intergenic 35.0%
!! AATATTTGGACTAGCAGCAA+TGG + chr4_1:32558801-32558820 None:intergenic 35.0%
GCTGCTATTACATCCATTAT+TGG - chr4_1:32558836-32558855 Msa0544310:CDS 35.0%
TGGGACTAAGTTTCAACTCA+AGG + chr4_1:32555080-32555099 None:intergenic 40.0%
TTCATGAGATGAGATTGGAG+AGG + chr4_1:32555162-32555181 None:intergenic 40.0%
TATGATCATTGTCAGGGACA+GGG + chr4_1:32555247-32555266 None:intergenic 40.0%
ATATGATCATTGTCAGGGAC+AGG + chr4_1:32555248-32555267 None:intergenic 40.0%
! GAAGAGAACTAAGAGTATGG+AGG - chr4_1:32555553-32555572 Msa0544310:CDS 40.0%
GACCCACGTACACAAAAATA+TGG - chr4_1:32555741-32555760 Msa0544310:CDS 40.0%
!! GCCCATATTTTTGTGTACGT+GGG + chr4_1:32555746-32555765 None:intergenic 40.0%
GTACACAAAAATATGGGCCA+AGG - chr4_1:32555748-32555767 Msa0544310:CDS 40.0%
TTAAAAGAAAGCGAGGACCT+TGG + chr4_1:32555768-32555787 None:intergenic 40.0%
! TGTGTGATTCTTGGTTGGTT+TGG - chr4_1:32555902-32555921 Msa0544310:intron 40.0%
GGATCATATTGGTGCAATGA+CGG + chr4_1:32556379-32556398 None:intergenic 40.0%
!! ACTTGGATTGGCTACTGATA+AGG - chr4_1:32556994-32557013 Msa0544310:CDS 40.0%
AGCTAGATCAGAAGAAACCA+TGG - chr4_1:32557438-32557457 Msa0544310:intron 40.0%
! AGATCACTGATGGATGTCAA+TGG - chr4_1:32557558-32557577 Msa0544310:intron 40.0%
GGATGTCAATGGAGAAAGTA+TGG - chr4_1:32557569-32557588 Msa0544310:intron 40.0%
CTGCAGCGATAATAAGCTTT+AGG + chr4_1:32557623-32557642 None:intergenic 40.0%
AACTTACATGCTTTCGGACT+GGG + chr4_1:32557661-32557680 None:intergenic 40.0%
AAACTTACATGCTTTCGGAC+TGG + chr4_1:32557662-32557681 None:intergenic 40.0%
GATTTGATATGCCTTGCACA+TGG - chr4_1:32557976-32557995 Msa0544310:intron 40.0%
TTGTTATCCAACCATGTGCA+AGG + chr4_1:32557990-32558009 None:intergenic 40.0%
TTACGCACCTAACACATCAA+TGG - chr4_1:32558103-32558122 Msa0544310:intron 40.0%
ATGGTCGTTGTATCGAAGAT+TGG + chr4_1:32558167-32558186 None:intergenic 40.0%
GCATCAACATCAAGCAATGA+TGG - chr4_1:32558461-32558480 Msa0544310:intron 40.0%
!! AACTGATTTGCGTTCCTTTG+TGG + chr4_1:32558608-32558627 None:intergenic 40.0%
! CCAAAACTCTGAGGGATTAA+AGG + chr4_1:32558638-32558657 None:intergenic 40.0%
! CCTTTAATCCCTCAGAGTTT+TGG - chr4_1:32558635-32558654 Msa0544310:intron 40.0%
GAGGAATTTCCAAAACTCTG+AGG + chr4_1:32558647-32558666 None:intergenic 40.0%
AGCTGACCCTTCACAAATAT+TGG - chr4_1:32558739-32558758 Msa0544310:intron 40.0%
GACAAAGCTGCAGGAATATT+TGG + chr4_1:32558815-32558834 None:intergenic 40.0%
TGGCTGAGTATCACCAATAA+TGG + chr4_1:32558852-32558871 None:intergenic 40.0%
!! GCTACCATTGCTATTTCCTT+CGG + chr4_1:32558924-32558943 None:intergenic 40.0%
ACATGTGGCATCAACAATGA+TGG - chr4_1:32558983-32559002 Msa0544310:CDS 40.0%
CATGTGGCATCAACAATGAT+GGG - chr4_1:32558984-32559003 Msa0544310:CDS 40.0%
GGACAGAGACAACTTCATGA+TGG + chr4_1:32555226-32555245 None:intergenic 45.0%
! GTATGGGTGTCACACATTGA+CGG + chr4_1:32555714-32555733 None:intergenic 45.0%
TCAATGTGTGACACCCATAC+AGG - chr4_1:32555714-32555733 Msa0544310:CDS 45.0%
CAATGTGTGACACCCATACA+GGG - chr4_1:32555715-32555734 Msa0544310:CDS 45.0%
!! GGCCCATATTTTTGTGTACG+TGG + chr4_1:32555747-32555766 None:intergenic 45.0%
AGAGTGATGCAAGACCGTAA+GGG - chr4_1:32556875-32556894 Msa0544310:intron 45.0%
! AACTGAGAATGGTGGAGTAC+TGG - chr4_1:32556952-32556971 Msa0544310:CDS 45.0%
! ACTGAGAATGGTGGAGTACT+GGG - chr4_1:32556953-32556972 Msa0544310:CDS 45.0%
ACTGGGGCCAAGGAAATTTA+TGG - chr4_1:32556970-32556989 Msa0544310:CDS 45.0%
!! TGCTGGATTGGCTACTGATA+AGG - chr4_1:32557114-32557133 Msa0544310:intron 45.0%
! ACTTCAGAATGGTGGAGAGT+TGG - chr4_1:32557192-32557211 Msa0544310:CDS 45.0%
!! GTTGGTGTCAAGGCAATGTA+AGG - chr4_1:32557210-32557229 Msa0544310:CDS 45.0%
TGATATGCCTTGCACATGGT+TGG - chr4_1:32557980-32557999 Msa0544310:intron 45.0%
GCAATGGCCATTGATGTGTT+AGG + chr4_1:32558113-32558132 None:intergenic 45.0%
GTGCTTATTACGTGAATGCC+TGG - chr4_1:32558191-32558210 Msa0544310:intron 45.0%
CTTTAGCTGCTGCAGGTAAA+GGG + chr4_1:32558397-32558416 None:intergenic 45.0%
TCTTTAGCTGCTGCAGGTAA+AGG + chr4_1:32558398-32558417 None:intergenic 45.0%
ACCTGCAGCAGCTAAAGAAA+TGG - chr4_1:32558400-32558419 Msa0544310:intron 45.0%
! GCGTTCCTTTGTGGAGTATT+TGG + chr4_1:32558599-32558618 None:intergenic 45.0%
AATGGCAACAATGTCACCGA+AGG - chr4_1:32558905-32558924 Msa0544310:intron 45.0%
GTCACCGAAGGAAATAGCAA+TGG - chr4_1:32558917-32558936 Msa0544310:intron 45.0%
! TGGCATCAACAATGATGGGA+AGG - chr4_1:32558988-32559007 Msa0544310:CDS 45.0%
TGATGGGAAGGAACGAAATC+AGG - chr4_1:32559000-32559019 Msa0544310:CDS 45.0%
!!! TTTTATATTTTTCTTTTATT+TGG - chr4_1:32556253-32556272 Msa0544310:intron 5.0%
GCAGAAATGCACGACTCTCA+TGG + chr4_1:32556001-32556020 None:intergenic 50.0%
GAGAGTGATGCAAGACCGTA+AGG - chr4_1:32556874-32556893 Msa0544310:intron 50.0%
!! CTGAGAATGGTGGAGTACTG+GGG - chr4_1:32556954-32556973 Msa0544310:CDS 50.0%
!! ATGGTGGAGAGTTGGTGTCA+AGG - chr4_1:32557200-32557219 Msa0544310:CDS 50.0%
GCCATTTCTTTAGCTGCTGC+AGG + chr4_1:32558404-32558423 None:intergenic 50.0%
TTGAGGGATCAGAGAAGCTG+AGG + chr4_1:32558666-32558685 None:intergenic 50.0%
ATAGCAGCTGACAAAGCTGC+AGG + chr4_1:32558824-32558843 None:intergenic 50.0%
! GCAGCAGCAGTGTTGTTGTT+TGG + chr4_1:32558872-32558891 None:intergenic 50.0%
ACGTGGGTCTGTCCCTGTAT+GGG + chr4_1:32555730-32555749 None:intergenic 55.0%
TACGTGGGTCTGTCCCTGTA+TGG + chr4_1:32555731-32555750 None:intergenic 55.0%
! CTTTCGGACTGGGCAACCAT+GGG + chr4_1:32557651-32557670 None:intergenic 55.0%
TAGCTGCTGCAGGTAAAGGG+TGG + chr4_1:32558394-32558413 None:intergenic 55.0%
!! ATGGTGGAGTACTGGGGCCA+AGG - chr4_1:32556960-32556979 Msa0544310:CDS 60.0%
TATCGCTGCAGCACGACCCA+TGG - chr4_1:32557632-32557651 Msa0544310:intron 60.0%
! GCTTTCGGACTGGGCAACCA+TGG + chr4_1:32557652-32557671 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 32555027 32559072 32555027 ID=Msa0544310;Name=Msa0544310
chr4_1 mRNA 32555027 32559072 32555027 ID=Msa0544310-mRNA-1;Parent=Msa0544310;Name=Msa0544310-mRNA-1;_AED=0.08;_eAED=0.09;_QI=0|0|0|1|0.57|0.37|8|0|565
chr4_1 exon 32558976 32559072 32558976 ID=Msa0544310-mRNA-1:exon:8338;Parent=Msa0544310-mRNA-1
chr4_1 exon 32558792 32558907 32558792 ID=Msa0544310-mRNA-1:exon:8337;Parent=Msa0544310-mRNA-1
chr4_1 exon 32558525 32558626 32558525 ID=Msa0544310-mRNA-1:exon:8336;Parent=Msa0544310-mRNA-1
chr4_1 exon 32557132 32557233 32557132 ID=Msa0544310-mRNA-1:exon:8335;Parent=Msa0544310-mRNA-1
chr4_1 exon 32556886 32557083 32556886 ID=Msa0544310-mRNA-1:exon:8334;Parent=Msa0544310-mRNA-1
chr4_1 exon 32556640 32556867 32556640 ID=Msa0544310-mRNA-1:exon:8333;Parent=Msa0544310-mRNA-1
chr4_1 exon 32556426 32556539 32556426 ID=Msa0544310-mRNA-1:exon:8332;Parent=Msa0544310-mRNA-1
chr4_1 exon 32555027 32555767 32555027 ID=Msa0544310-mRNA-1:exon:8331;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32558976 32559072 32558976 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32558792 32558907 32558792 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32558525 32558626 32558525 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32557132 32557233 32557132 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32556886 32557083 32556886 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32556640 32556867 32556640 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32556426 32556539 32556426 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
chr4_1 CDS 32555027 32555767 32555027 ID=Msa0544310-mRNA-1:cds;Parent=Msa0544310-mRNA-1
Gene Sequence

>Msa0544310

ATGGCTTCAGTTCCAGTTAATAACAACTCTTTCCCTGATGATACTAACAACCTTGAGTTGAAACTTAGTCCCAACAAAAACATGGATGCTACACATAAGTTGGATTTCTTTGATGACAACAACAATGACCATCATGAAGTTGTCTCTGTCCCTGTCCCTGACAATGATCATATTCATACTGTTACACCATCTTTATTGGAACTAAAGCTAAGCCTTGGTCCCATTCCTATTACTATCAATACTAGTAGTGATCAATCTATGATGGATGATGGCATGTCACCTAATTCAGAAGAGAAGAGAACTAAGAGTATGGAGCGTTTCGAGAGAGTGATGCAAGACCGTAAGGGTAAGAAAGTTAAAGGAAAAAAAGTCTTTGATGGAAAATTTAAAGAGAAAAAGAGAACTGAGAATGGTGGAGTTAAGGAAGTTAAAGGACAAAAAGTTTCTGATCAAAAATTTGTAGAGAAAATGAGAATTAAGAATGATGGAGAATTAATGAAAAGGAAATTTGATGATGCTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGAAAAGAAGTGTTCACTGGAAAATGTGAAAAGAAAAAGAGACTTCAGAATGGTGGAGAGTTGGATCTCGTGTTGATTACAAATGCTGATATTGTGATGGATCACGAAGCTTCGCCCTCGTCAATGAGAAAATCTAGAAGTCAAGATCAGTTAGGATCGGCGATGAAAAACATCGATGTTGCTTCGAATGAGATAGTCCTAAGTAAGAATGAGGATGTTAATGTTGATAATGTTGAGGATTCATTGACAAAAGCAAGAGTTTGTATTAGAGCTAGATCAGAAGAAACCATGATCACTGATGGATGTCAATGGAGAAAGTATGGGCAAAAACTATCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACGACCCATGGTTGCCCAGTCCGAAAGCATGTACAAAGGTGTGCTTTTGACAAAATAGTTGTAATCACAACTTATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCTAAAGAAATGGCAAAAACAACTTCAGCAGTTGCAAAGATACTTCTTTCAGCATCAACATCAAGCAATGATGGACAATTAAATGCAAATTTACTCACAAGAACACTTCTTCCTTGTTCTTCAAGTATTGCAACTATATCAGCTTCTGCTCCATATCCAACTATTACAATTGATTACACTCAATCTCCAAATACTCCACAAAGGAACGCAAATCAGTTTCAAACTCCTTTAATCCCTCAGAGTTTTGGAAATTCCTCAGCTTCTCTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACATATGTCGAAAGATGCAGCTGACCCTTCACAAATATTGGCAATACCAAATATTACTGCTCAAACAATTAATGCTGCCATTGCTGCTAGTCCAAATATTCCTGCAGCTTTGTCAGCTGCTATTACATCCATTATTGGTGATACTCAGCCAAACAACAACACTGCTGCTGCTGATAATAATAATAATGGCAACAATGTCACCGAAGGAAATAGCAATGGTAGCAACAATAATGTCACAGAAGCCAATAGCAACAAAATTATCACATGTGGCATCAACAATGATGGGAAGGAACGAAATCAGGAATACCAATTACAAGACCTAAACTTTAATTTTTTTTGCTCAAAGATTTAG
Protein sequence

>Msa0544310

MASVPVNNNSFPDDTNNLELKLSPNKNMDATHKLDFFDDNNNDHHEVVSVPVPDNDHIHTVTPSLLELKLSLGPIPITINTSSDQSMMDDGMSPNSEEKRTKSMERFERVMQDRKGKKVKGKKVFDGKFKEKKRTENGGVKEVKGQKVSDQKFVEKMRIKNDGELMKRKFDDAGLATDKVKEVKGKEVFTGKCEKKKRLQNGGELDLVLITNADIVMDHEASPSSMRKSRSQDQLGSAMKNIDVASNEIVLSKNEDVNVDNVEDSLTKARVCIRARSEETMITDGCQWRKYGQKLSKGNPCPKAYYRCSTTHGCPVRKHVQRCAFDKIVVITTYEGNHNHPLPAAAKEMAKTTSAVAKILLSASTSSNDGQLNANLLTRTLLPCSSSIATISASAPYPTITIDYTQSPNTPQRNANQFQTPLIPQSFGNSSASLIPQIPNQNQSKFSGLHMSKDAADPSQILAIPNITAQTINAAIAASPNIPAALSAAITSIIGDTQPNNNTAAADNNNNGNNVTEGNSNGSNNNVTEANSNKIITCGINNDGKERNQEYQLQDLNFNFFCSKI*