AlfalfaGEDB | Msa0544550

M. sativa cultivar zhongmu-4 / Msa0544550

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544550	XP_039687084.1	78.689	122	13	2	4	112	1	122	1.33e-59	189

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544550	G7INX6	78.689	122	13	2	4	112	1	122	6.34e-60	189

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TFs/TRs:

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Gene ID	Type	Classification
Msa0544550	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544550	MtrunA17_Chr2g0281511	78.689	122	13	2	4	112	1	122	1.22e-63	189
Msa0544550	MtrunA17_Chr2g0309121	72.951	122	19	3	4	112	1	121	2.54e-56	170
Msa0544550	MtrunA17_Chr3g0081891	75.000	80	7	2	4	70	1	80	3.12e-34	113
Msa0544550	MtrunA17_Chr7g0276661	47.059	68	35	1	43	110	101	167	6.09e-17	72.0
Msa0544550	MtrunA17_Chr5g0430431	42.623	61	35	0	46	106	194	254	5.16e-13	63.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 19 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATCGATGTTCATTAT+TGG	0.232793	4_1:+33241453	Msa0544550:CDS
AAATCAGTCCCAGTTAACGA+CGG	0.717617	4_1:-33241817	None:intergenic
AAGTCAATGGTACTGATTTA+GGG	0.335811	4_1:+33241423	Msa0544550:CDS
ACGGTAACATACATCCTATT+AGG	0.430681	4_1:-33241798	None:intergenic
AGAAATACATAGGGAGAGGA+TGG	0.573439	4_1:+33241709	Msa0544550:CDS
AGCATGCATCAGAAATACAT+AGG	0.544935	4_1:+33241699	Msa0544550:CDS
CATCAGAAATACATAGGGAG+AGG	0.580360	4_1:+33241705	Msa0544550:CDS
CGGTCAATAATCCTCCTAAT+AGG	0.355854	4_1:+33241784	Msa0544550:CDS
CTTGAAAAGGGCCTAAAATT+TGG	0.185070	4_1:+33241744	Msa0544550:CDS
GAAGTCAATGGTACTGATTT+AGG	0.173353	4_1:+33241422	Msa0544550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TACCAGTTTCTTCATCAAAA+AGG	-	chr4_1:33241644-33241663	None:intergenic	30.0%
TACGACTTTGTTCTTGAAAA+GGG	+	chr4_1:33241732-33241751	Msa0544550:CDS	30.0%
GAAGGAAAAATGAACGAGAT+TGG	+	chr4_1:33241395-33241414	Msa0544550:intron	35.0%
TTTGGATGGTAACAACGTTT+AGG	+	chr4_1:33241499-33241518	Msa0544550:intron	35.0%
AGCATGCATCAGAAATACAT+AGG	+	chr4_1:33241699-33241718	Msa0544550:CDS	35.0%
GCATGCATCAGAAATACATA+GGG	+	chr4_1:33241700-33241719	Msa0544550:CDS	35.0%
GTACGACTTTGTTCTTGAAA+AGG	+	chr4_1:33241731-33241750	Msa0544550:CDS	35.0%
CTTGAAAAGGGCCTAAAATT+TGG	+	chr4_1:33241744-33241763	Msa0544550:CDS	35.0%
GTAACATACATCCTATTAGG+AGG	-	chr4_1:33241798-33241817	None:intergenic	35.0%
ACGGTAACATACATCCTATT+AGG	-	chr4_1:33241801-33241820	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	33241271	33241836	33241271	ID=Msa0544550;Name=Msa0544550
chr4_1	mRNA	33241271	33241836	33241271	ID=Msa0544550-mRNA-1;Parent=Msa0544550;Name=Msa0544550-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|0.66\|0\|0\|3\|0\|112
chr4_1	exon	33241271	33241274	33241271	ID=Msa0544550-mRNA-1:exon:8512;Parent=Msa0544550-mRNA-1
chr4_1	exon	33241399	33241514	33241399	ID=Msa0544550-mRNA-1:exon:8513;Parent=Msa0544550-mRNA-1
chr4_1	exon	33241618	33241836	33241618	ID=Msa0544550-mRNA-1:exon:8514;Parent=Msa0544550-mRNA-1
chr4_1	CDS	33241271	33241274	33241271	ID=Msa0544550-mRNA-1:cds;Parent=Msa0544550-mRNA-1
chr4_1	CDS	33241399	33241514	33241399	ID=Msa0544550-mRNA-1:cds;Parent=Msa0544550-mRNA-1
chr4_1	CDS	33241618	33241836	33241618	ID=Msa0544550-mRNA-1:cds;Parent=Msa0544550-mRNA-1

Gene Sequence	>Msa0544550 ATGGGAAAAATGAACGAGATTGGAGTTGAAGTCAATGGTACTGATTTAGGGAATGTTCAAAATATCGATGTTCATTATTGGAAGTATGATGTTACTACTTCTATTTTGGATGGTAACAACCATTTCAATCAATCTAAAATTCTCCTTTTTGATGAAGAAACTGGTAAATCTTATGACTGTGATGTGTACGCTGCGTCAAAAAGCATGCATCAGAAATACATAGGGAGAGGATGGTACGACTTTGTTCTTGAAAAGGGCCTAAAATTTGGTGACGTTATTTTGTTCACGGTCAATAATCCTCCTAATAGGATGTATGTTACCGTCGTTAACTGGGACTGA
Protein sequence	>Msa0544550 MGKMNEIGVEVNGTDLGNVQNIDVHYWKYDVTTSILDGNNHFNQSKILLFDEETGKSYDCDVYAASKSMHQKYIGRGWYDFVLEKGLKFGDVILFTVNNPPNRMYVTVVNWD*