AlfalfaGEDB | Msa0544580

M. sativa cultivar zhongmu-4 / Msa0544580

KEGG: K14491
GO: -
PFAM: PF00072.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544580	XP_013455007.2	87.500	312	38	1	1	311	1	312	0.0	565

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544580	sp\|Q5N6V8\|ORR26_ORYSJ	39.918	243	118	4	6	220	2	244	4.83e-54	188

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544580	A0A396I0H3	87.500	312	38	1	1	311	1	312	0.0	565

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TFs/TRs:

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Gene ID	Type	Classification
Msa0544580	TF	GARP-ARR-B

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544580	MtrunA17_Chr4g0009851	87.500	312	38	1	1	311	1	312	0.0	565
Msa0544580	MtrunA17_Chr4g0009981	84.170	259	41	0	53	311	1	259	5.55e-166	461
Msa0544580	MtrunA17_Chr4g0009901	71.250	240	69	0	1	240	1	240	4.65e-121	348
Msa0544580	MtrunA17_Chr4g0011071	78.102	137	30	0	55	191	35	171	1.78e-76	231
Msa0544580	MtrunA17_Chr6g0471041	46.711	304	141	8	1	294	1	293	8.49e-74	233
Msa0544580	MtrunA17_Chr1g0161431	36.944	360	162	8	13	307	14	373	3.64e-66	218
Msa0544580	MtrunA17_Chr3g0123131	40.678	236	112	3	13	220	16	251	6.77e-58	195
Msa0544580	MtrunA17_Chr4g0009991	76.068	117	27	1	101	217	1	116	1.57e-56	179
Msa0544580	MtrunA17_Chr4g0067981	41.026	234	112	2	13	220	34	267	1.97e-56	193
Msa0544580	MtrunA17_Chr2g0294911	38.433	268	139	3	13	254	32	299	2.94e-56	191

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0544580	AT2G01760.1	38.039	255	119	5	13	230	10	262	4.22e-54	181
Msa0544580	AT1G67710.1	41.772	237	109	4	13	220	10	246	8.07e-54	184
Msa0544580	AT3G16857.1	38.976	254	109	6	13	220	36	289	1.54e-50	177
Msa0544580	AT3G16857.2	38.976	254	109	6	13	220	36	289	1.89e-50	177
Msa0544580	AT2G01760.2	34.276	283	119	6	13	230	10	290	4.02e-48	166
Msa0544580	AT4G31920.1	36.179	246	142	4	13	246	16	258	3.67e-47	166
Msa0544580	AT2G25180.1	36.638	232	123	3	13	220	16	247	5.81e-44	159
Msa0544580	AT5G58080.1	38.793	232	116	5	13	220	17	246	2.47e-40	149
Msa0544580	AT3G16857.3	37.615	218	90	6	49	220	2	219	5.49e-39	145
Msa0544580	AT2G01760.3	35.678	199	90	4	68	230	1	197	1.39e-36	133

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Find 55 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGAACCTGTAAATGGC+TGG	0.537566	4_1:+33414084	None:intergenic
AAAAGAAACACATGCAAAGA+AGG	0.478150	4_1:-33414329	Msa0544580:CDS
AAAAGTGTTGCTCATAATGT+TGG	0.434928	4_1:+33413400	None:intergenic
AAGCTAAATTGTGTTTCAAG+AGG	0.513615	4_1:+33413533	None:intergenic
AAGTCATAAACATCCATGTC+TGG	0.491105	4_1:+33414700	None:intergenic
AATAAATTCTTACTAATGAC+AGG	0.461511	4_1:+33414646	None:intergenic
AATGTGGGGCTTGCGAATAT+TGG	0.385194	4_1:-33414498	Msa0544580:CDS
ACATCTCCTCGACGAAATTA+AGG	0.321564	4_1:+33414935	None:intergenic
ACATGTTGCCAGCCATTTAC+AGG	0.283862	4_1:-33414092	Msa0544580:intron
AGAAAACTTTGGTCCTGCTC+CGG	0.527471	4_1:+33413491	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGTAATCGATGCAATTATA+AGG	-	chr4_1:33413435-33413454	Msa0544580:CDS	30.0%
TATGAAGTCTATTGAATGTG+GGG	-	chr4_1:33413837-33413856	Msa0544580:intron	30.0%
TGGCAACATGTTATAAGAAA+AGG	-	chr4_1:33413910-33413929	Msa0544580:intron	30.0%
ATATGAAATTGTTGGAAGCT+CGG	-	chr4_1:33413951-33413970	Msa0544580:intron	30.0%
AAAAGAAACACATGCAAAGA+AGG	-	chr4_1:33414020-33414039	Msa0544580:intron	30.0%
AGGAAAGAATCATACTATCA+AGG	+	chr4_1:33414107-33414126	None:intergenic	30.0%
TACAAAAACGAACCTGTAAA+TGG	+	chr4_1:33414272-33414291	None:intergenic	30.0%
AGAATTACAACAACAGACTT+AGG	+	chr4_1:33414369-33414388	None:intergenic	30.0%
TTGTATAACATGCAAGACTA+AGG	+	chr4_1:33414415-33414434	None:intergenic	30.0%
AGAGGATATATAGAAGCTAA+CGG	+	chr4_1:33414625-33414644	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	33413340	33415031	33413340	ID=Msa0544580;Name=Msa0544580
chr4_1	mRNA	33413340	33415031	33413340	ID=Msa0544580-mRNA-1;Parent=Msa0544580;Name=Msa0544580-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0\|0\|1\|1\|1\|5\|0\|311
chr4_1	exon	33414914	33415031	33414914	ID=Msa0544580-mRNA-1:exon:8553;Parent=Msa0544580-mRNA-1
chr4_1	exon	33414659	33414808	33414659	ID=Msa0544580-mRNA-1:exon:8552;Parent=Msa0544580-mRNA-1
chr4_1	exon	33414260	33414565	33414260	ID=Msa0544580-mRNA-1:exon:8551;Parent=Msa0544580-mRNA-1
chr4_1	exon	33414093	33414169	33414093	ID=Msa0544580-mRNA-1:exon:8550;Parent=Msa0544580-mRNA-1
chr4_1	exon	33413340	33413624	33413340	ID=Msa0544580-mRNA-1:exon:8549;Parent=Msa0544580-mRNA-1
chr4_1	CDS	33414914	33415031	33414914	ID=Msa0544580-mRNA-1:cds;Parent=Msa0544580-mRNA-1
chr4_1	CDS	33414659	33414808	33414659	ID=Msa0544580-mRNA-1:cds;Parent=Msa0544580-mRNA-1
chr4_1	CDS	33414260	33414565	33414260	ID=Msa0544580-mRNA-1:cds;Parent=Msa0544580-mRNA-1
chr4_1	CDS	33414093	33414169	33414093	ID=Msa0544580-mRNA-1:cds;Parent=Msa0544580-mRNA-1
chr4_1	CDS	33413340	33413624	33413340	ID=Msa0544580-mRNA-1:cds;Parent=Msa0544580-mRNA-1

Gene Sequence

>Msa0544580

ATGGCCCTTTTCACCGACAAACCATCATTATCAGAAGGTCTCAAGGTTCTTGCAATTGATCATGACATCACTCTCCTTAATTTCGTCGAGGAGATGTGTAATCGATGCAATTATAAGGTTACCAAATGTAGCATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATGTTGATTGATGCTCATATGCCAGACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAAAATTCCTGTCATTATGATGGCCGTTGATTCTGCAGAAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAACAGTTCAAGACCATGTGGCAACATGTTATAAGAAAAGGTTTGACTCAAAACAAAAAATATGAAATTGTTGGAAGCTCGGTGTTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACATGCAAAGAAGGCCCGTTTGTCTTGGTCGCGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATTATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGAAGTATAGAAATCAGTTTAAGAGCTCAACCGATGAGACAAAAGGTAAGATGAAAAAATCAAAGAAGAGTCCTCTTGAAACACAATTTAGCTTAGAAGCTACTCACTCAATACCGGAGCAGGACCAAAGTTTTCTTCATAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACAACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCTGACTTTCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA

Protein sequence

>Msa0544580

MALFTDKPSLSEGLKVLAIDHDITLLNFVEEMCNRCNYKVTKCSMASDAFNMLERKDCFDVMLIDAHMPDMDVYDFVQNVTLQLKIPVIMMAVDSAESSIMKSIECGACEYWTKPLVEKQFKTMWQHVIRKGLTQNKKYEIVGSSVFQENRKRGREDANASKETHAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHVASHLQKYRNQFKSSTDETKGKMKKSKKSPLETQFSLEATHSIPEQDQSFLHNSATQCRDNCDAQQHWVEIGESNIMSNTFSDFPNLFPYVDDDLSSLVW*