AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0545160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545160 MtrunA17_Chr4g0025671 83.966 474 24 4 5 450 140 589 0.0 716
Msa0545160 MtrunA17_Chr2g0321841 52.016 496 159 8 5 450 136 602 1.34e-139 425
Msa0545160 MtrunA17_Chr7g0258051 61.702 141 32 2 5 137 148 274 3.71e-49 177
Msa0545160 MtrunA17_Chr7g0260191 47.692 195 78 3 5 189 136 316 4.18e-46 169
Msa0545160 MtrunA17_Chr1g0173921 51.235 162 54 3 5 155 136 283 8.67e-45 167
Msa0545160 MtrunA17_Chr1g0185221 54.610 141 42 2 5 137 136 262 1.18e-43 161
Msa0545160 MtrunA17_Chr1g0190831 56.028 141 40 2 5 137 136 262 5.19e-43 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545160 AT3G44200.2 45.674 497 184 14 5 448 140 603 3.48e-100 321
Msa0545160 AT3G44200.1 45.674 497 184 14 5 448 140 603 3.48e-100 321
Msa0545160 AT3G20860.1 49.010 202 63 5 5 180 135 322 1.31e-48 172
Msa0545160 AT3G04810.2 41.423 239 109 5 5 227 136 359 8.65e-43 159
Msa0545160 AT3G04810.1 41.423 239 109 5 5 227 136 359 8.65e-43 159
Msa0545160 AT3G04810.3 41.423 239 109 5 5 227 136 359 8.65e-43 159
Msa0545160 AT5G28290.1 55.245 143 42 2 5 139 136 264 2.02e-42 158
Msa0545160 AT5G28290.2 55.245 143 42 2 5 139 136 264 2.02e-42 158
Msa0545160 AT5G28290.3 55.245 143 42 2 5 139 136 264 2.10e-42 157
Msa0545160 AT1G54510.4 48.851 174 65 3 5 168 136 295 9.47e-42 157
Msa0545160 AT1G54510.2 48.851 174 65 3 5 168 136 295 9.47e-42 157
Msa0545160 AT1G54510.1 48.851 174 65 3 5 168 136 295 9.47e-42 157
Msa0545160 AT1G54510.3 48.851 174 65 3 5 168 136 295 9.47e-42 157
Msa0545160 AT3G63280.2 44.624 186 76 3 5 182 136 302 1.12e-39 150
Msa0545160 AT3G63280.3 44.624 186 76 3 5 182 136 302 1.12e-39 150
Msa0545160 AT3G63280.1 44.624 186 76 3 5 182 136 302 1.12e-39 150
Msa0545160 AT3G63280.4 44.624 186 76 3 5 182 190 356 3.91e-39 149
Msa0545160 AT3G12200.4 51.807 166 50 6 5 158 85 232 7.54e-39 147
Msa0545160 AT3G12200.3 51.807 166 50 6 5 158 79 226 8.39e-39 147
Msa0545160 AT3G12200.1 51.807 166 50 6 5 158 151 298 1.34e-38 147
Msa0545160 AT3G12200.2 51.807 166 50 6 5 158 161 308 1.48e-38 147
Msa0545160 AT1G54510.5 42.308 130 59 2 41 168 7 122 5.09e-22 98.6

Find 197 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTCTTGCAAAACTTTC+TGG 0.009957 4_1:-34978809 None:intergenic
TCATCATCAGTGACTGTTTC+AGG 0.153805 4_1:-34978711 None:intergenic
TTCGTCTTCGGTTTCTATTC+AGG 0.162823 4_1:+34978150 Msa0545160:CDS
GAAACTTACAAATGCTTTAA+AGG 0.168763 4_1:-34976282 None:intergenic
ATGGATTCAAATCTGATATT+TGG 0.240589 4_1:+34976133 Msa0545160:CDS
TAATAGTATTCTGAAAATTC+AGG 0.255432 4_1:-34978350 None:intergenic
AAATCTGATATTTGGTCATT+AGG 0.261155 4_1:+34976141 Msa0545160:CDS
TCATGTTTCGGTTTAACTTT+TGG 0.272477 4_1:-34977809 None:intergenic
GGTGGTTTGTCTTTGGTTGT+AGG 0.277626 4_1:-34979283 None:intergenic
TCGACGTAAGGTTGCAAATA+AGG 0.300528 4_1:-34976946 None:intergenic
CGGTAAACAAACTCAATAAT+AGG 0.303834 4_1:+34978688 Msa0545160:three_prime_UTR
AATATTAGATTTCAAGGTTT+CGG 0.308659 4_1:-34979081 None:intergenic
TTCAGGAAAATACTAATAAA+AGG 0.319552 4_1:+34976770 Msa0545160:intron
ATTTCAACCGATTCCATTAC+CGG 0.322677 4_1:-34978948 None:intergenic
ATCAGAAATATTAGATTTCA+AGG 0.341302 4_1:-34979087 None:intergenic
AGGGTCATCAAGTGTTAAAT+TGG 0.349154 4_1:-34978394 None:intergenic
TCCTCTATCAAACAAGTAGA+AGG 0.351103 4_1:+34977848 Msa0545160:CDS
CCGCTATCATTCGCTGCATT+CGG 0.352371 4_1:-34977968 None:intergenic
AAACATCCACACTCAACTTC+TGG 0.352468 4_1:+34978636 Msa0545160:three_prime_UTR
TCAGGAGAAAAGGCTGTTAG+AGG 0.352592 4_1:-34976991 None:intergenic
ACCAAATTGCCGTCTCCCTT+GGG 0.353764 4_1:-34979453 None:intergenic
TGCTTTAGTAGAGGTAATTA+CGG 0.358862 4_1:-34978781 None:intergenic
GATACAGTTGTCTGATTTCT+CGG 0.360307 4_1:-34978459 None:intergenic
CCAACTTACGGTTAATTGTA+TGG 0.365409 4_1:+34979710 Msa0545160:three_prime_UTR
GATTCCTGGCCTGTATTACT+TGG 0.367389 4_1:-34978001 None:intergenic
CAAAGACAAACCACCGGAAA+AGG 0.367673 4_1:+34979291 Msa0545160:three_prime_UTR
GTATCTGGCCTTTCTTGTGC+TGG 0.376523 4_1:-34978849 None:intergenic
CCCCAAGGGAGACGGCAATT+TGG 0.377388 4_1:+34979452 Msa0545160:three_prime_UTR
TCGAGATACTTCTAATGAAA+TGG 0.378388 4_1:+34978596 Msa0545160:three_prime_UTR
CCCTTCTACTTGTTTGATAG+AGG 0.379922 4_1:-34977849 None:intergenic
TCGTCTTCGGTTTCTATTCA+GGG 0.380498 4_1:+34978151 Msa0545160:CDS
CAAGTTGTACTAAAGCCATT+TGG 0.380531 4_1:+34979415 Msa0545160:three_prime_UTR
GGAATATCTGCAAGAAGTTC+AGG 0.386094 4_1:-34976108 None:intergenic
ACAGTCGGTGACTCTAAATT+AGG 0.389190 4_1:-34977456 None:intergenic
CTTGGAGGAGTTCTTTGTCT+TGG 0.393351 4_1:-34977890 None:intergenic
GCAGGAAGATGACTCGTATC+TGG 0.395717 4_1:-34978864 None:intergenic
AGGCGAATATCTTGATCCTT+TGG 0.397030 4_1:-34975790 None:intergenic
CTTGTTACATTGTCATGATC+AGG 0.397789 4_1:-34978228 None:intergenic
GGTAATGGAATCGGTTGAAA+TGG 0.398626 4_1:+34978950 Msa0545160:three_prime_UTR
TGGTTAGCAAGGAGTCTTAA+GGG 0.399625 4_1:+34979472 Msa0545160:three_prime_UTR
AATGGCTCATCTCAGATTCC+TGG 0.400891 4_1:-34978015 None:intergenic
AACCAAATTGCCGTCTCCCT+TGG 0.405393 4_1:-34979454 None:intergenic
ATCTAATATTTCTGATCAGT+TGG 0.409279 4_1:+34979094 Msa0545160:three_prime_UTR
AGCCATCGTACAACGCGAAC+AGG 0.409773 4_1:-34978295 None:intergenic
TTTCTTTCAAAAGCCTTATG+AGG 0.415236 4_1:-34978091 None:intergenic
TTGGTTAGCAAGGAGTCTTA+AGG 0.416421 4_1:+34979471 Msa0545160:three_prime_UTR
AGCCATTGTCATTTGTTCAA+TGG 0.416571 4_1:+34978031 Msa0545160:CDS
GCAAGTTTATCAATGTTATG+AGG 0.417047 4_1:+34978994 Msa0545160:three_prime_UTR
CCAGGACGAATGAGGCTGGC+AGG 0.417801 4_1:+34977935 Msa0545160:CDS
GGAATGATGTCCGAGGAAAA+TGG 0.419416 4_1:+34979493 Msa0545160:three_prime_UTR
TAATATCAAAAGCAGATAGA+TGG 0.419834 4_1:-34976531 None:intergenic
GATATGTCTGTATTTACTTT+CGG 0.419868 4_1:-34977390 None:intergenic
TCCCAACGCGGTAACTACTA+TGG 0.421582 4_1:-34978917 None:intergenic
GGTGGAGTCATATCATGTTT+CGG 0.422902 4_1:-34977821 None:intergenic
TACTTGACGTGAAATCATTC+AGG 0.424624 4_1:+34979317 Msa0545160:three_prime_UTR
GGGGAATAACATGGAGGTAA+AGG 0.425758 4_1:-34976494 None:intergenic
TCGAAGACCTTCGATTTCTT+CGG 0.427217 4_1:-34978577 None:intergenic
GGAGTTCTTTGTCTTGGCCT+AGG 0.434310 4_1:-34977884 None:intergenic
TTATGAGGTTCTCTAGTAAT+CGG 0.434399 4_1:-34978076 None:intergenic
AAATTTGAGGATTTAAGTGT+AGG 0.436962 4_1:-34979146 None:intergenic
GGAGCAATGAATGTAGGTTT+AGG 0.438138 4_1:-34977423 None:intergenic
TAGTAATTCTGCTTCGTCTT+CGG 0.448074 4_1:+34978138 Msa0545160:CDS
CACTTATACTAAAAGACATA+TGG 0.451761 4_1:+34977032 Msa0545160:CDS
CTTACAAATGCTTTAAAGGC+TGG 0.453294 4_1:-34976278 None:intergenic
TCTTACTGTTGGTCGATGCT+CGG 0.453886 4_1:-34976810 None:intergenic
GGGAATAACATGGAGGTAAA+GGG 0.456639 4_1:-34976493 None:intergenic
CTTGTTGCATTTACTGTGCT+CGG 0.457155 4_1:-34979260 None:intergenic
AAGATTTAAGAGTCATTTGA+GGG 0.460747 4_1:-34979043 None:intergenic
AAACTAGGAGCAATGAATGT+AGG 0.460761 4_1:-34977429 None:intergenic
CTTACTGTTGGTCGATGCTC+GGG 0.461929 4_1:-34976809 None:intergenic
ATTTCTCGGAAGACTATCTT+CGG 0.462285 4_1:-34978445 None:intergenic
TGGGAGTGATCAGCCGGTAA+TGG 0.463915 4_1:+34978935 Msa0545160:three_prime_UTR
AGAAGCACTTGAAGAGCTCT+TGG 0.464570 4_1:+34979348 Msa0545160:three_prime_UTR
GGTGAAGGACCGATTGTCAT+TGG 0.464879 4_1:+34978743 Msa0545160:three_prime_UTR
AACAATTATAAATATAATGA+TGG 0.465215 4_1:+34977260 Msa0545160:CDS
TGAGGATTTAAGTGTAGGAG+GGG 0.471680 4_1:-34979141 None:intergenic
CGCAGAATCCAGCACAAGAA+AGG 0.474796 4_1:+34978841 Msa0545160:three_prime_UTR
AAGATAAACCGTTCCTCCAT+TGG 0.474857 4_1:+34976470 Msa0545160:CDS
AATAGTATTCTGAAAATTCA+GGG 0.474918 4_1:-34978349 None:intergenic
TTGAGGATTTAAGTGTAGGA+GGG 0.476755 4_1:-34979142 None:intergenic
TTTGAGGATTTAAGTGTAGG+AGG 0.477286 4_1:-34979143 None:intergenic
ATAATGATGGCATTGAAAGA+AGG 0.478367 4_1:+34977273 Msa0545160:CDS
GCTTTAGTAGAGGTAATTAC+GGG 0.478491 4_1:-34978780 None:intergenic
GTTGCTGCAACATAATAGAT+TGG 0.479035 4_1:+34979384 Msa0545160:three_prime_UTR
TAATGATGGCATTGAAAGAA+GGG 0.481349 4_1:+34977274 Msa0545160:CDS
TCCTTTGGTAAGAAAGATGT+TGG 0.482038 4_1:-34975775 None:intergenic
GCCATAGTAGTTACCGCGTT+GGG 0.489304 4_1:+34978916 Msa0545160:three_prime_UTR
GTATAAGTGACAGATGAAAT+CGG 0.494011 4_1:-34977018 None:intergenic
AAAATTCAGGGTGACATCAC+AGG 0.494480 4_1:-34978337 None:intergenic
GAAGATTTAAGAGTCATTTG+AGG 0.494581 4_1:-34979044 None:intergenic
CCGAATGCAGCGAATGATAG+CGG 0.495993 4_1:+34977968 Msa0545160:CDS
CTTCTTGCAGATATTCCGTA+TGG 0.497681 4_1:+34976114 Msa0545160:CDS
TGCTATAAATGTTTCTGTGC+AGG 0.498542 4_1:+34976059 Msa0545160:CDS
AAGTCTGTTTGTGAAGAAGT+TGG 0.500290 4_1:+34978663 Msa0545160:three_prime_UTR
AAGCTAGGTCCAAGTAATAC+AGG 0.503473 4_1:+34977992 Msa0545160:CDS
TGTATGGACATTCTGTGCCT+TGG 0.503570 4_1:+34979726 Msa0545160:three_prime_UTR
GTTGTCGACGGACTACTGTC+TGG 0.506045 4_1:-34978273 None:intergenic
CAAGTTTATCAATGTTATGA+GGG 0.508093 4_1:+34978995 Msa0545160:three_prime_UTR
CGAATGCAGCGAATGATAGC+GGG 0.508182 4_1:+34977969 Msa0545160:CDS
TGGCGCATCGGAGAAATTTG+AGG 0.509038 4_1:-34979159 None:intergenic
TGATCAGCCGGTAATGGAAT+CGG 0.514489 4_1:+34978941 Msa0545160:three_prime_UTR
GTGGCTTTGTTATCACACTT+TGG 0.514654 4_1:-34977135 None:intergenic
TCCAACATCTTTCTTACCAA+AGG 0.515537 4_1:+34975774 Msa0545160:CDS
CACTGATGATGACAAATTCA+CGG 0.517222 4_1:+34978722 Msa0545160:three_prime_UTR
GATGAGAGGCAGTCGTCTAA+AGG 0.517537 4_1:+34977350 Msa0545160:CDS
AAAGCCATTTGGAAAAGATA+AGG 0.522753 4_1:+34979426 Msa0545160:three_prime_UTR
TGTTTGTGAAGAAGTTGGTT+CGG 0.523583 4_1:+34978668 Msa0545160:three_prime_UTR
GGACATTCTGTGCCTTGGAA+TGG 0.526599 4_1:+34979731 Msa0545160:three_prime_UTR
GTAATGGAATCGGTTGAAAT+GGG 0.527581 4_1:+34978951 Msa0545160:three_prime_UTR
GATTACTAGAGAACCTCATA+AGG 0.528851 4_1:+34978078 Msa0545160:CDS
CCTGCCAGCCTCATTCGTCC+TGG 0.529518 4_1:-34977935 None:intergenic
GTAGGCCGCCGAAGTAAGCT+TGG 0.530589 4_1:-34977908 None:intergenic
AATGTTTCTGTGCAGGTGGT+AGG 0.531181 4_1:+34976066 Msa0545160:CDS
TCTAAAGGCTGGTGGAGTAT+CGG 0.532128 4_1:+34977365 Msa0545160:CDS
CAGGACGAATGAGGCTGGCA+GGG 0.533831 4_1:+34977936 Msa0545160:CDS
ACTTGTTTGATAGAGGAAGA+TGG 0.536538 4_1:-34977842 None:intergenic
CTCTATCAAACAAGTAGAAG+GGG 0.539412 4_1:+34977850 Msa0545160:CDS
GCGAATGATAGCGGGAAGCT+AGG 0.540673 4_1:+34977977 Msa0545160:CDS
CCTCTATCAAACAAGTAGAA+GGG 0.542560 4_1:+34977849 Msa0545160:CDS
GGATTTAAGTGTAGGAGGGG+TGG 0.542907 4_1:-34979138 None:intergenic
AATATTTAACAACCATTCCA+AGG 0.543154 4_1:-34979743 None:intergenic
AAGGGGATGGAGTTCCTCCT+AGG 0.544687 4_1:+34977867 Msa0545160:CDS
ATCACATCATCAAAGGCTGC+AGG 0.550620 4_1:-34978882 None:intergenic
AACTACTATGGCGTATAACA+TGG 0.551076 4_1:-34978905 None:intergenic
TTTCAAGGTTTCGGCTTCAG+AGG 0.551358 4_1:-34979072 None:intergenic
GCTGTTAGAGGAGCAAAAGA+TGG 0.552104 4_1:-34976979 None:intergenic
CCATACAATTAACCGTAAGT+TGG 0.553138 4_1:-34979710 None:intergenic
CTGGCTCGATGCTTTAGTAG+AGG 0.553978 4_1:-34978790 None:intergenic
TGTTGATACATCTACGGAAG+TGG 0.554545 4_1:+34978554 Msa0545160:three_prime_UTR
CACATAGAAGCAGTAGATGT+AGG 0.555579 4_1:-34978519 None:intergenic
CTCATTCGTCCTGGAAAAGT+AGG 0.556113 4_1:-34977926 None:intergenic
ATCAAACAAGTAGAAGGGGA+TGG 0.556933 4_1:+34977854 Msa0545160:CDS
GCCTTTACAGGTGGAACAGT+CGG 0.557558 4_1:-34977471 None:intergenic
CCGCGTTGGGAGTGATCAGC+CGG 0.558172 4_1:+34978929 Msa0545160:three_prime_UTR
AGAGGCAGTCGTCTAAAGGC+TGG 0.560302 4_1:+34977354 Msa0545160:CDS
ATATCAGATTTGAATCCATA+CGG 0.561816 4_1:-34976129 None:intergenic
TCTAAATTAGGCTTCAAACT+AGG 0.563558 4_1:-34977444 None:intergenic
CAAATAATAATACCAACTTA+CGG 0.564441 4_1:+34979698 Msa0545160:three_prime_UTR
GTGTGGATGTTTAAGAGACT+TGG 0.566035 4_1:-34978625 None:intergenic
AGGTCCAAGTAATACAGGCC+AGG 0.567392 4_1:+34977997 Msa0545160:CDS
TTTCAGCGACCAATGACAAT+CGG 0.568520 4_1:-34978752 None:intergenic
TAGATATGTTGATACATCTA+CGG 0.570383 4_1:+34978548 Msa0545160:three_prime_UTR
TAAGGTCTCCCCAAGGGAGA+CGG 0.573298 4_1:+34979444 Msa0545160:three_prime_UTR
GGAGTTCAGAATGTCGATGT+TGG 0.574763 4_1:+34978972 Msa0545160:three_prime_UTR
GGCCGCCGAAGTAAGCTTGG+AGG 0.577111 4_1:-34977905 None:intergenic
GCTTCTCTACCGCCTCTCAT+CGG 0.578510 4_1:-34977318 None:intergenic
CGCCATAGTAGTTACCGCGT+TGG 0.578558 4_1:+34978915 Msa0545160:three_prime_UTR
GTGATTTCAAGCTGGCGCAT+CGG 0.579122 4_1:-34979171 None:intergenic
GAACTCCTCCAAGCTTACTT+CGG 0.580119 4_1:+34977900 Msa0545160:CDS
TGATAACAAAGCCACTGAGA+TGG 0.581140 4_1:+34977143 Msa0545160:CDS
AGACGGCAATTTGGTTAGCA+AGG 0.581863 4_1:+34979461 Msa0545160:three_prime_UTR
ACACTGTTACTCACATCTTG+AGG 0.584924 4_1:-34979211 None:intergenic
ACATGGAGGTAAAGGGCCAA+TGG 0.593183 4_1:-34976486 None:intergenic
TTTAGACGACTGCCTCTCAT+CGG 0.597265 4_1:-34977348 None:intergenic
AACCTGTTCGCGTTGTACGA+TGG 0.599342 4_1:+34978293 Msa0545160:three_prime_UTR
AACATGGATCACATCATCAA+AGG 0.599734 4_1:-34978889 None:intergenic
ATTATGTTACAGGATATGGC+AGG 0.603089 4_1:+34976437 Msa0545160:intron
TACAACCAAAGACAAACCAC+CGG 0.606631 4_1:+34979285 Msa0545160:three_prime_UTR
TAGATGGATACTTGCAACGA+GGG 0.607313 4_1:-34976515 None:intergenic
GTAAAGGGCCAATGGAGGAA+CGG 0.610196 4_1:-34976478 None:intergenic
AGATGGATACTTGCAACGAG+GGG 0.613484 4_1:-34976514 None:intergenic
GGACGGTATTGATCGACGTA+AGG 0.616267 4_1:-34976958 None:intergenic
CCAAATTGCCGTCTCCCTTG+GGG 0.617375 4_1:-34979452 None:intergenic
GCTGCAACATAATAGATTGG+AGG 0.619279 4_1:+34979387 Msa0545160:three_prime_UTR
AATGCTCGGTGATTTCAAGC+TGG 0.624316 4_1:-34979179 None:intergenic
GACAATGTAACAAGAAGTGT+CGG 0.624487 4_1:+34978237 Msa0545160:three_prime_UTR
GGAGGACGTTGTTCAAGATG+AGG 0.626144 4_1:+34979514 Msa0545160:three_prime_UTR
AAGGATCAAGATATTCGCCT+CGG 0.628650 4_1:+34975793 Msa0545160:CDS
ATGGCATTGAAAGAAGGGAA+AGG 0.629525 4_1:+34977279 Msa0545160:CDS
GAAGCAACTTCTCCGATGAG+AGG 0.630458 4_1:+34977336 Msa0545160:CDS
GGCAGTCGTCTAAAGGCTGG+TGG 0.630647 4_1:+34977357 Msa0545160:CDS
TGATGACAAATTCACGGTGA+AGG 0.631985 4_1:+34978728 Msa0545160:three_prime_UTR
ATTGCATTGATCGATGATGA+TGG 0.632392 4_1:+34977168 Msa0545160:CDS
GTTCTTTGTCTTGGCCTAGG+AGG 0.633038 4_1:-34977881 None:intergenic
TGTTTGATAGAGGAAGATGG+TGG 0.634388 4_1:-34977839 None:intergenic
GAAGCAACTTCTCCGATGAG+AGG 0.634560 4_1:+34977306 Msa0545160:CDS
TGCCATTGAACAAATGACAA+TGG 0.640279 4_1:-34978033 None:intergenic
AAAAGATAAGGTCTCCCCAA+GGG 0.641634 4_1:+34979438 Msa0545160:three_prime_UTR
TATAAATGTTTCTGTGCAGG+TGG 0.655428 4_1:+34976062 Msa0545160:CDS
AGGCTGCTGTGTATACGAGA+TGG 0.656965 4_1:+34976244 Msa0545160:intron
ACAATAATGAAAATAAGCAG+CGG 0.661126 4_1:-34979633 None:intergenic
GAAAAGATAAGGTCTCCCCA+AGG 0.663233 4_1:+34979437 Msa0545160:three_prime_UTR
GCAACTTCTCCGATGAGAGG+CGG 0.664594 4_1:+34977309 Msa0545160:CDS
GATGGCTGAAGAAGTGTCAG+AGG 0.667967 4_1:+34978311 Msa0545160:three_prime_UTR
CAACGCGAACAGGTTGTCGA+CGG 0.669582 4_1:-34978285 None:intergenic
TTAGAGGAGCAAAAGATGGA+CGG 0.671178 4_1:-34976975 None:intergenic
TGATGATGGCTCTGAGCAAG+AGG 0.677140 4_1:+34977182 Msa0545160:CDS
CTCCTCCAAGCTTACTTCGG+CGG 0.677899 4_1:+34977903 Msa0545160:CDS
TGGAGGTAAAGGGCCAATGG+AGG 0.682510 4_1:-34976483 None:intergenic
GATGGATACTTGCAACGAGG+GGG 0.686733 4_1:-34976513 None:intergenic
CAATGCAATATCCATCTCAG+TGG 0.709191 4_1:-34977154 None:intergenic
ATGATGTCCGAGGAAAATGG+AGG 0.716276 4_1:+34979496 Msa0545160:three_prime_UTR
TCTTAAGGGAATGATGTCCG+AGG 0.718372 4_1:+34979486 Msa0545160:three_prime_UTR
CCGGCTGATCACTCCCAACG+CGG 0.723578 4_1:-34978929 None:intergenic
GAAGTGGCCGAAGAAATCGA+AGG 0.740516 4_1:+34978570 Msa0545160:three_prime_UTR
GTAATCGGTCTAGAAATACG+AGG 0.784585 4_1:-34978061 None:intergenic
ATAGATGGATACTTGCAACG+AGG 0.788431 4_1:-34976516 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTATCAAAATCAA+CGG - chr4_1:34976722-34976741 None:intergenic 10.0%
!! AAATAAATCATTTCTAAATT+GGG - chr4_1:34976856-34976875 None:intergenic 10.0%
!! TAAATAAATCATTTCTAAAT+TGG - chr4_1:34976857-34976876 None:intergenic 10.0%
!! AACAATTATAAATATAATGA+TGG + chr4_1:34977260-34977279 Msa0545160:CDS 10.0%
!! AATTATTTACTTCTATTTAT+TGG + chr4_1:34977762-34977781 Msa0545160:intron 10.0%
!! ATATCACATGTAATAAAAAA+TGG - chr4_1:34975831-34975850 None:intergenic 15.0%
!!! TAAATAATTTTAATGCTATG+AGG - chr4_1:34977751-34977770 None:intergenic 15.0%
!! AGAAAAATATAGACAAAGTT+AGG - chr4_1:34975881-34975900 None:intergenic 20.0%
!!! TAACTTTGTCTATATTTTTC+TGG + chr4_1:34975880-34975899 Msa0545160:intron 20.0%
!!! GTATTAGATTTTTATGTATG+TGG + chr4_1:34976366-34976385 Msa0545160:intron 20.0%
!!! AATTTTTTTCATTGAAACTG+TGG + chr4_1:34976733-34976752 Msa0545160:intron 20.0%
!! TTCAGGAAAATACTAATAAA+AGG + chr4_1:34976770-34976789 Msa0545160:intron 20.0%
!!! AATAGTATTCTGAAAATTCA+GGG - chr4_1:34978352-34978371 None:intergenic 20.0%
!!! TAATAGTATTCTGAAAATTC+AGG - chr4_1:34978353-34978372 None:intergenic 20.0%
!!! TTTTCTTAGAATTATCAACA+GGG - chr4_1:34978416-34978435 None:intergenic 20.0%
!!! ATTTTCTTAGAATTATCAAC+AGG - chr4_1:34978417-34978436 None:intergenic 20.0%
!! AATATTAGATTTCAAGGTTT+CGG - chr4_1:34979084-34979103 None:intergenic 20.0%
!! ATCAGAAATATTAGATTTCA+AGG - chr4_1:34979090-34979109 None:intergenic 20.0%
!! CAAATAATAATACCAACTTA+CGG + chr4_1:34979698-34979717 Msa0545160:three_prime_UTR 20.0%
! ATATCAGATTTGAATCCATA+CGG - chr4_1:34976132-34976151 None:intergenic 25.0%
! ATGGATTCAAATCTGATATT+TGG + chr4_1:34976133-34976152 Msa0545160:CDS 25.0%
! AAATCTGATATTTGGTCATT+AGG + chr4_1:34976141-34976160 Msa0545160:CDS 25.0%
!!! TGAGATTTTGTTGTTTATTC+AGG + chr4_1:34976224-34976243 Msa0545160:intron 25.0%
! GAAACTTACAAATGCTTTAA+AGG - chr4_1:34976285-34976304 None:intergenic 25.0%
! AAAACACAAGAAATTACACA+TGG - chr4_1:34976402-34976421 None:intergenic 25.0%
! TAATATCAAAAGCAGATAGA+TGG - chr4_1:34976534-34976553 None:intergenic 25.0%
!! AAATATAGTGAGCTTGTTTA+GGG - chr4_1:34976897-34976916 None:intergenic 25.0%
!! AAAATATAGTGAGCTTGTTT+AGG - chr4_1:34976898-34976917 None:intergenic 25.0%
! CACTTATACTAAAAGACATA+TGG + chr4_1:34977032-34977051 Msa0545160:CDS 25.0%
! GATATGTCTGTATTTACTTT+CGG - chr4_1:34977393-34977412 None:intergenic 25.0%
!!! GGTATATTACCTTTTGAAAT+TGG + chr4_1:34977545-34977564 Msa0545160:intron 25.0%
! CAATCAAATCCAATTTCAAA+AGG - chr4_1:34977557-34977576 None:intergenic 25.0%
! TAGATATGTTGATACATCTA+CGG + chr4_1:34978548-34978567 Msa0545160:three_prime_UTR 25.0%
! CAAGTTTATCAATGTTATGA+GGG + chr4_1:34978995-34979014 Msa0545160:three_prime_UTR 25.0%
! AAGATTTAAGAGTCATTTGA+GGG - chr4_1:34979046-34979065 None:intergenic 25.0%
!! ATCTAATATTTCTGATCAGT+TGG + chr4_1:34979094-34979113 Msa0545160:three_prime_UTR 25.0%
! AAATTTGAGGATTTAAGTGT+AGG - chr4_1:34979149-34979168 None:intergenic 25.0%
! CTACTTAAGAGAAAAATGTT+TGG + chr4_1:34979605-34979624 Msa0545160:three_prime_UTR 25.0%
! ACAATAATGAAAATAAGCAG+CGG - chr4_1:34979636-34979655 None:intergenic 25.0%
! AATATTTAACAACCATTCCA+AGG - chr4_1:34979746-34979765 None:intergenic 25.0%
! TTCTGGCATATATGTGATTT+TGG + chr4_1:34975897-34975916 Msa0545160:intron 30.0%
GACACTAAAACAAGATGATT+TGG + chr4_1:34975929-34975948 Msa0545160:intron 30.0%
CAACAAAATCTCATTTCAGT+CGG - chr4_1:34976217-34976236 None:intergenic 30.0%
CGTAATTATGTTACAGGATA+TGG + chr4_1:34976433-34976452 Msa0545160:intron 30.0%
TTGTTTAGGGGAAAAAAACA+AGG - chr4_1:34976884-34976903 None:intergenic 30.0%
! AATATAGTGAGCTTGTTTAG+GGG - chr4_1:34976896-34976915 None:intergenic 30.0%
GTATAAGTGACAGATGAAAT+CGG - chr4_1:34977021-34977040 None:intergenic 30.0%
! ATAATGATGGCATTGAAAGA+AGG + chr4_1:34977273-34977292 Msa0545160:CDS 30.0%
! TAATGATGGCATTGAAAGAA+GGG + chr4_1:34977274-34977293 Msa0545160:CDS 30.0%
TCTAAATTAGGCTTCAAACT+AGG - chr4_1:34977447-34977466 None:intergenic 30.0%
!!! ATATCCGTTTTGAAGAATCA+GGG - chr4_1:34977500-34977519 None:intergenic 30.0%
!!! TATATCCGTTTTGAAGAATC+AGG - chr4_1:34977501-34977520 None:intergenic 30.0%
GATTCTTCAAAACGGATATA+TGG + chr4_1:34977501-34977520 Msa0545160:intron 30.0%
TAAGTCACATGATTGATTGA+TGG + chr4_1:34977670-34977689 Msa0545160:intron 30.0%
! AGGAAATCTTTGAAAATAGC+TGG - chr4_1:34977731-34977750 None:intergenic 30.0%
!! TCATGTTTCGGTTTAACTTT+TGG - chr4_1:34977812-34977831 None:intergenic 30.0%
TTATGAGGTTCTCTAGTAAT+CGG - chr4_1:34978079-34978098 None:intergenic 30.0%
! TTTCTTTCAAAAGCCTTATG+AGG - chr4_1:34978094-34978113 None:intergenic 30.0%
TCGAGATACTTCTAATGAAA+TGG + chr4_1:34978596-34978615 Msa0545160:three_prime_UTR 30.0%
CGGTAAACAAACTCAATAAT+AGG + chr4_1:34978688-34978707 Msa0545160:three_prime_UTR 30.0%
TGCTTTAGTAGAGGTAATTA+CGG - chr4_1:34978784-34978803 None:intergenic 30.0%
GCAAGTTTATCAATGTTATG+AGG + chr4_1:34978994-34979013 Msa0545160:three_prime_UTR 30.0%
GAAGATTTAAGAGTCATTTG+AGG - chr4_1:34979047-34979066 None:intergenic 30.0%
AAAGCCATTTGGAAAAGATA+AGG + chr4_1:34979426-34979445 Msa0545160:three_prime_UTR 30.0%
TCCAACATCTTTCTTACCAA+AGG + chr4_1:34975774-34975793 Msa0545160:CDS 35.0%
TCCTTTGGTAAGAAAGATGT+TGG - chr4_1:34975778-34975797 None:intergenic 35.0%
! ACAAGATGATTTGGCTTCTT+CGG + chr4_1:34975938-34975957 Msa0545160:intron 35.0%
! TGCTATAAATGTTTCTGTGC+AGG + chr4_1:34976059-34976078 Msa0545160:CDS 35.0%
! TATAAATGTTTCTGTGCAGG+TGG + chr4_1:34976062-34976081 Msa0545160:CDS 35.0%
!! CTTACAAATGCTTTAAAGGC+TGG - chr4_1:34976281-34976300 None:intergenic 35.0%
AAACAGTGTAGAGAATCACA+TGG - chr4_1:34976345-34976364 None:intergenic 35.0%
ATGCGTCGTAATTATGTTAC+AGG + chr4_1:34976427-34976446 Msa0545160:intron 35.0%
ATTATGTTACAGGATATGGC+AGG + chr4_1:34976437-34976456 Msa0545160:intron 35.0%
AAGTCTGATTTCGTCGAAAA+GGG - chr4_1:34976582-34976601 None:intergenic 35.0%
TAAGTCTGATTTCGTCGAAA+AGG - chr4_1:34976583-34976602 None:intergenic 35.0%
CACAGAAAACAGAAACACAT+GGG - chr4_1:34976637-34976656 None:intergenic 35.0%
ACACAGAAAACAGAAACACA+TGG - chr4_1:34976638-34976657 None:intergenic 35.0%
GCACTTAACCACATTGTTAT+AGG + chr4_1:34976664-34976683 Msa0545160:intron 35.0%
AAATGCGACCTATAACAATG+TGG - chr4_1:34976675-34976694 None:intergenic 35.0%
AATCTCGAGATTCTTACTGT+TGG - chr4_1:34976824-34976843 None:intergenic 35.0%
! AGCTCACTATATTTTCTCAC+AGG + chr4_1:34976903-34976922 Msa0545160:intron 35.0%
!!! AATCGGTTTTTCAGGAGAAA+AGG - chr4_1:34977004-34977023 None:intergenic 35.0%
!! ACAGATGAAATCGGTTTTTC+AGG - chr4_1:34977012-34977031 None:intergenic 35.0%
! ATTGCATTGATCGATGATGA+TGG + chr4_1:34977168-34977187 Msa0545160:CDS 35.0%
AAACTAGGAGCAATGAATGT+AGG - chr4_1:34977432-34977451 None:intergenic 35.0%
!!! TATCCGTTTTGAAGAATCAG+GGG - chr4_1:34977499-34977518 None:intergenic 35.0%
!! TTCGGTTTAACTTTTGGTGA+GGG - chr4_1:34977806-34977825 None:intergenic 35.0%
!! TTTCGGTTTAACTTTTGGTG+AGG - chr4_1:34977807-34977826 None:intergenic 35.0%
ACTTGTTTGATAGAGGAAGA+TGG - chr4_1:34977845-34977864 None:intergenic 35.0%
TCCTCTATCAAACAAGTAGA+AGG + chr4_1:34977848-34977867 Msa0545160:CDS 35.0%
CCTCTATCAAACAAGTAGAA+GGG + chr4_1:34977849-34977868 Msa0545160:CDS 35.0%
CTCTATCAAACAAGTAGAAG+GGG + chr4_1:34977850-34977869 Msa0545160:CDS 35.0%
! AGCCATTGTCATTTGTTCAA+TGG + chr4_1:34978031-34978050 Msa0545160:CDS 35.0%
TGCCATTGAACAAATGACAA+TGG - chr4_1:34978036-34978055 None:intergenic 35.0%
GATTACTAGAGAACCTCATA+AGG + chr4_1:34978078-34978097 Msa0545160:CDS 35.0%
TAGTAATTCTGCTTCGTCTT+CGG + chr4_1:34978138-34978157 Msa0545160:CDS 35.0%
CTTGTTACATTGTCATGATC+AGG - chr4_1:34978231-34978250 None:intergenic 35.0%
! GACAATGTAACAAGAAGTGT+CGG + chr4_1:34978237-34978256 Msa0545160:three_prime_UTR 35.0%
AGGGTCATCAAGTGTTAAAT+TGG - chr4_1:34978397-34978416 None:intergenic 35.0%
ATTTCTCGGAAGACTATCTT+CGG - chr4_1:34978448-34978467 None:intergenic 35.0%
GATACAGTTGTCTGATTTCT+CGG - chr4_1:34978462-34978481 None:intergenic 35.0%
AAGTCTGTTTGTGAAGAAGT+TGG + chr4_1:34978663-34978682 Msa0545160:three_prime_UTR 35.0%
! TGTTTGTGAAGAAGTTGGTT+CGG + chr4_1:34978668-34978687 Msa0545160:three_prime_UTR 35.0%
! CACTGATGATGACAAATTCA+CGG + chr4_1:34978722-34978741 Msa0545160:three_prime_UTR 35.0%
GCTTTAGTAGAGGTAATTAC+GGG - chr4_1:34978783-34978802 None:intergenic 35.0%
CTTTCTCTTGCAAAACTTTC+TGG - chr4_1:34978812-34978831 None:intergenic 35.0%
! CAGAAAGTTTTGCAAGAGAA+AGG + chr4_1:34978810-34978829 Msa0545160:three_prime_UTR 35.0%
AACATGGATCACATCATCAA+AGG - chr4_1:34978892-34978911 None:intergenic 35.0%
AACTACTATGGCGTATAACA+TGG - chr4_1:34978908-34978927 None:intergenic 35.0%
! ATTTCAACCGATTCCATTAC+CGG - chr4_1:34978951-34978970 None:intergenic 35.0%
! GTAATGGAATCGGTTGAAAT+GGG + chr4_1:34978951-34978970 Msa0545160:three_prime_UTR 35.0%
TTGAGGATTTAAGTGTAGGA+GGG - chr4_1:34979145-34979164 None:intergenic 35.0%
TTTGAGGATTTAAGTGTAGG+AGG - chr4_1:34979146-34979165 None:intergenic 35.0%
! TGAGGATTTTCAGAAATGCT+CGG - chr4_1:34979196-34979215 None:intergenic 35.0%
! ACGTCAAGTATTTCCTTTTC+CGG - chr4_1:34979307-34979326 None:intergenic 35.0%
TACTTGACGTGAAATCATTC+AGG + chr4_1:34979317-34979336 Msa0545160:three_prime_UTR 35.0%
GTTGCTGCAACATAATAGAT+TGG + chr4_1:34979384-34979403 Msa0545160:three_prime_UTR 35.0%
CAAGTTGTACTAAAGCCATT+TGG + chr4_1:34979415-34979434 Msa0545160:three_prime_UTR 35.0%
! GAGACCTTATCTTTTCCAAA+TGG - chr4_1:34979433-34979452 None:intergenic 35.0%
CCATACAATTAACCGTAAGT+TGG - chr4_1:34979713-34979732 None:intergenic 35.0%
CCAACTTACGGTTAATTGTA+TGG + chr4_1:34979710-34979729 Msa0545160:three_prime_UTR 35.0%
AGGCGAATATCTTGATCCTT+TGG - chr4_1:34975793-34975812 None:intergenic 40.0%
AAGGATCAAGATATTCGCCT+CGG + chr4_1:34975793-34975812 Msa0545160:CDS 40.0%
AATGGTAGATGAACTAACCG+AGG - chr4_1:34975813-34975832 None:intergenic 40.0%
!! CACTTGTTAGCTTGTGGTTA+AGG - chr4_1:34975989-34976008 None:intergenic 40.0%
! GAAATGCACTTGTTAGCTTG+TGG - chr4_1:34975995-34976014 None:intergenic 40.0%
GGAATATCTGCAAGAAGTTC+AGG - chr4_1:34976111-34976130 None:intergenic 40.0%
CTTCTTGCAGATATTCCGTA+TGG + chr4_1:34976114-34976133 Msa0545160:CDS 40.0%
GCATCTGATACTTGCAGATT+TGG + chr4_1:34976184-34976203 Msa0545160:intron 40.0%
AAGATAAACCGTTCCTCCAT+TGG + chr4_1:34976470-34976489 Msa0545160:CDS 40.0%
GGGAATAACATGGAGGTAAA+GGG - chr4_1:34976496-34976515 None:intergenic 40.0%
TAGATGGATACTTGCAACGA+GGG - chr4_1:34976518-34976537 None:intergenic 40.0%
ATAGATGGATACTTGCAACG+AGG - chr4_1:34976519-34976538 None:intergenic 40.0%
AAACACATGGGTGCAAACTA+GGG - chr4_1:34976625-34976644 None:intergenic 40.0%
TGGATCTGTTTACTTGCTTC+AGG + chr4_1:34976753-34976772 Msa0545160:intron 40.0%
! TATTTTCTCACAGGCATCTG+AGG + chr4_1:34976912-34976931 Msa0545160:intron 40.0%
TCGACGTAAGGTTGCAAATA+AGG - chr4_1:34976949-34976968 None:intergenic 40.0%
TTAGAGGAGCAAAAGATGGA+CGG - chr4_1:34976978-34976997 None:intergenic 40.0%
!! GTGGCTTTGTTATCACACTT+TGG - chr4_1:34977138-34977157 None:intergenic 40.0%
TGATAACAAAGCCACTGAGA+TGG + chr4_1:34977143-34977162 Msa0545160:CDS 40.0%
CAATGCAATATCCATCTCAG+TGG - chr4_1:34977157-34977176 None:intergenic 40.0%
! ATGGCATTGAAAGAAGGGAA+AGG + chr4_1:34977279-34977298 Msa0545160:CDS 40.0%
GGAGCAATGAATGTAGGTTT+AGG - chr4_1:34977426-34977445 None:intergenic 40.0%
ACAGTCGGTGACTCTAAATT+AGG - chr4_1:34977459-34977478 None:intergenic 40.0%
CATCCCCTGATTCTTCAAAA+CGG + chr4_1:34977493-34977512 Msa0545160:intron 40.0%
ATCACATCAGTCAAAGCTAC+AGG + chr4_1:34977524-34977543 Msa0545160:intron 40.0%
GGTGGAGTCATATCATGTTT+CGG - chr4_1:34977824-34977843 None:intergenic 40.0%
TGTTTGATAGAGGAAGATGG+TGG - chr4_1:34977842-34977861 None:intergenic 40.0%
CCCTTCTACTTGTTTGATAG+AGG - chr4_1:34977852-34977871 None:intergenic 40.0%
ATCAAACAAGTAGAAGGGGA+TGG + chr4_1:34977854-34977873 Msa0545160:CDS 40.0%
AAGCTAGGTCCAAGTAATAC+AGG + chr4_1:34977992-34978011 Msa0545160:CDS 40.0%
GTAATCGGTCTAGAAATACG+AGG - chr4_1:34978064-34978083 None:intergenic 40.0%
TTCGTCTTCGGTTTCTATTC+AGG + chr4_1:34978150-34978169 Msa0545160:CDS 40.0%
TCGTCTTCGGTTTCTATTCA+GGG + chr4_1:34978151-34978170 Msa0545160:CDS 40.0%
AAAATTCAGGGTGACATCAC+AGG - chr4_1:34978340-34978359 None:intergenic 40.0%
CACATAGAAGCAGTAGATGT+AGG - chr4_1:34978522-34978541 None:intergenic 40.0%
TGTTGATACATCTACGGAAG+TGG + chr4_1:34978554-34978573 Msa0545160:three_prime_UTR 40.0%
TCGAAGACCTTCGATTTCTT+CGG - chr4_1:34978580-34978599 None:intergenic 40.0%
GTGTGGATGTTTAAGAGACT+TGG - chr4_1:34978628-34978647 None:intergenic 40.0%
AAACATCCACACTCAACTTC+TGG + chr4_1:34978636-34978655 Msa0545160:three_prime_UTR 40.0%
! TTTTCTCCAGAAGTTGAGTG+TGG - chr4_1:34978645-34978664 None:intergenic 40.0%
TCATCATCAGTGACTGTTTC+AGG - chr4_1:34978714-34978733 None:intergenic 40.0%
TGATGACAAATTCACGGTGA+AGG + chr4_1:34978728-34978747 Msa0545160:three_prime_UTR 40.0%
TTTCAGCGACCAATGACAAT+CGG - chr4_1:34978755-34978774 None:intergenic 40.0%
!! GGTAATGGAATCGGTTGAAA+TGG + chr4_1:34978950-34978969 Msa0545160:three_prime_UTR 40.0%
TGAGGATTTAAGTGTAGGAG+GGG - chr4_1:34979144-34979163 None:intergenic 40.0%
ACACTGTTACTCACATCTTG+AGG - chr4_1:34979214-34979233 None:intergenic 40.0%
CTTGTTGCATTTACTGTGCT+CGG - chr4_1:34979263-34979282 None:intergenic 40.0%
TACAACCAAAGACAAACCAC+CGG + chr4_1:34979285-34979304 Msa0545160:three_prime_UTR 40.0%
! TCAAGTATTTCCTTTTCCGG+TGG - chr4_1:34979304-34979323 None:intergenic 40.0%
GCTGCAACATAATAGATTGG+AGG + chr4_1:34979387-34979406 Msa0545160:three_prime_UTR 40.0%
AAAAGATAAGGTCTCCCCAA+GGG + chr4_1:34979438-34979457 Msa0545160:three_prime_UTR 40.0%
TTGGTTAGCAAGGAGTCTTA+AGG + chr4_1:34979471-34979490 Msa0545160:three_prime_UTR 40.0%
TGGTTAGCAAGGAGTCTTAA+GGG + chr4_1:34979472-34979491 Msa0545160:three_prime_UTR 40.0%
! AATGTTTCTGTGCAGGTGGT+AGG + chr4_1:34976066-34976085 Msa0545160:CDS 45.0%
GGGGAATAACATGGAGGTAA+AGG - chr4_1:34976497-34976516 None:intergenic 45.0%
AGATGGATACTTGCAACGAG+GGG - chr4_1:34976517-34976536 None:intergenic 45.0%
GAAACACATGGGTGCAAACT+AGG - chr4_1:34976626-34976645 None:intergenic 45.0%
TCTTACTGTTGGTCGATGCT+CGG - chr4_1:34976813-34976832 None:intergenic 45.0%
GCTGTTAGAGGAGCAAAAGA+TGG - chr4_1:34976982-34977001 None:intergenic 45.0%
TCAGGAGAAAAGGCTGTTAG+AGG - chr4_1:34976994-34977013 None:intergenic 45.0%
TTTAGACGACTGCCTCTCAT+CGG - chr4_1:34977351-34977370 None:intergenic 45.0%
TCTAAAGGCTGGTGGAGTAT+CGG + chr4_1:34977365-34977384 Msa0545160:CDS 45.0%
CTTGGAGGAGTTCTTTGTCT+TGG - chr4_1:34977893-34977912 None:intergenic 45.0%
GAACTCCTCCAAGCTTACTT+CGG + chr4_1:34977900-34977919 Msa0545160:CDS 45.0%
CTCATTCGTCCTGGAAAAGT+AGG - chr4_1:34977929-34977948 None:intergenic 45.0%
!! CTACTTTTCCAGGACGAATG+AGG + chr4_1:34977927-34977946 Msa0545160:CDS 45.0%
GATTCCTGGCCTGTATTACT+TGG - chr4_1:34978004-34978023 None:intergenic 45.0%
AATGGCTCATCTCAGATTCC+TGG - chr4_1:34978018-34978037 None:intergenic 45.0%
ATCACATCATCAAAGGCTGC+AGG - chr4_1:34978885-34978904 None:intergenic 45.0%
!! TGATCAGCCGGTAATGGAAT+CGG + chr4_1:34978941-34978960 Msa0545160:three_prime_UTR 45.0%
GGAGTTCAGAATGTCGATGT+TGG + chr4_1:34978972-34978991 Msa0545160:three_prime_UTR 45.0%
TTTCAAGGTTTCGGCTTCAG+AGG - chr4_1:34979075-34979094 None:intergenic 45.0%
! AATGCTCGGTGATTTCAAGC+TGG - chr4_1:34979182-34979201 None:intergenic 45.0%
! GGTGGTTTGTCTTTGGTTGT+AGG - chr4_1:34979286-34979305 None:intergenic 45.0%
!! CTTTTCCGGTGGTTTGTCTT+TGG - chr4_1:34979293-34979312 None:intergenic 45.0%
CAAAGACAAACCACCGGAAA+AGG + chr4_1:34979291-34979310 Msa0545160:three_prime_UTR 45.0%
!! AGAAGCACTTGAAGAGCTCT+TGG + chr4_1:34979348-34979367 Msa0545160:three_prime_UTR 45.0%
GAAAAGATAAGGTCTCCCCA+AGG + chr4_1:34979437-34979456 Msa0545160:three_prime_UTR 45.0%
AGACGGCAATTTGGTTAGCA+AGG + chr4_1:34979461-34979480 Msa0545160:three_prime_UTR 45.0%
TCTTAAGGGAATGATGTCCG+AGG + chr4_1:34979486-34979505 Msa0545160:three_prime_UTR 45.0%
GGAATGATGTCCGAGGAAAA+TGG + chr4_1:34979493-34979512 Msa0545160:three_prime_UTR 45.0%
ATGATGTCCGAGGAAAATGG+AGG + chr4_1:34979496-34979515 Msa0545160:three_prime_UTR 45.0%
! ACAACGTCCTCCATTTTCCT+CGG - chr4_1:34979506-34979525 None:intergenic 45.0%
TGTATGGACATTCTGTGCCT+TGG + chr4_1:34979726-34979745 Msa0545160:three_prime_UTR 45.0%
!!! ATTATATTTATAATTGTTTT+TGG - chr4_1:34977258-34977277 None:intergenic 5.0%
AGGCTGCTGTGTATACGAGA+TGG + chr4_1:34976244-34976263 Msa0545160:intron 50.0%
GTAAAGGGCCAATGGAGGAA+CGG - chr4_1:34976481-34976500 None:intergenic 50.0%
ACATGGAGGTAAAGGGCCAA+TGG - chr4_1:34976489-34976508 None:intergenic 50.0%
AACGAGGGGGAATAACATGG+AGG - chr4_1:34976503-34976522 None:intergenic 50.0%
TGCAACGAGGGGGAATAACA+TGG - chr4_1:34976506-34976525 None:intergenic 50.0%
GATGGATACTTGCAACGAGG+GGG - chr4_1:34976516-34976535 None:intergenic 50.0%
CTTACTGTTGGTCGATGCTC+GGG - chr4_1:34976812-34976831 None:intergenic 50.0%
!! GGACGGTATTGATCGACGTA+AGG - chr4_1:34976961-34976980 None:intergenic 50.0%
!! TGATGATGGCTCTGAGCAAG+AGG + chr4_1:34977182-34977201 Msa0545160:CDS 50.0%
GAAGCAACTTCTCCGATGAG+AGG + chr4_1:34977306-34977325 Msa0545160:CDS 50.0%
GAAGCAACTTCTCCGATGAG+AGG + chr4_1:34977306-34977325 Msa0545160:CDS 50.0%
GATGAGAGGCAGTCGTCTAA+AGG + chr4_1:34977350-34977369 Msa0545160:CDS 50.0%
ACCGACTGTTCCACCTGTAA+AGG + chr4_1:34977470-34977489 Msa0545160:intron 50.0%
GCCTTTACAGGTGGAACAGT+CGG - chr4_1:34977474-34977493 None:intergenic 50.0%
GAATCAGGGGATGCCTTTAC+AGG - chr4_1:34977486-34977505 None:intergenic 50.0%
GTTCTTTGTCTTGGCCTAGG+AGG - chr4_1:34977884-34977903 None:intergenic 50.0%
GGAGTTCTTTGTCTTGGCCT+AGG - chr4_1:34977887-34977906 None:intergenic 50.0%
! TTTTCCAGGACGAATGAGGC+TGG + chr4_1:34977931-34977950 Msa0545160:CDS 50.0%
CCGCTATCATTCGCTGCATT+CGG - chr4_1:34977971-34977990 None:intergenic 50.0%
CCGAATGCAGCGAATGATAG+CGG + chr4_1:34977968-34977987 Msa0545160:CDS 50.0%
CGAATGCAGCGAATGATAGC+GGG + chr4_1:34977969-34977988 Msa0545160:CDS 50.0%
AGGTCCAAGTAATACAGGCC+AGG + chr4_1:34977997-34978016 Msa0545160:CDS 50.0%
AACCTGTTCGCGTTGTACGA+TGG + chr4_1:34978293-34978312 Msa0545160:three_prime_UTR 50.0%
!! GATGGCTGAAGAAGTGTCAG+AGG + chr4_1:34978311-34978330 Msa0545160:three_prime_UTR 50.0%
GAAGTGGCCGAAGAAATCGA+AGG + chr4_1:34978570-34978589 Msa0545160:three_prime_UTR 50.0%
GGTGAAGGACCGATTGTCAT+TGG + chr4_1:34978743-34978762 Msa0545160:three_prime_UTR 50.0%
! CTGGCTCGATGCTTTAGTAG+AGG - chr4_1:34978793-34978812 None:intergenic 50.0%
CGCAGAATCCAGCACAAGAA+AGG + chr4_1:34978841-34978860 Msa0545160:three_prime_UTR 50.0%
! GTATCTGGCCTTTCTTGTGC+TGG - chr4_1:34978852-34978871 None:intergenic 50.0%
GCAGGAAGATGACTCGTATC+TGG - chr4_1:34978867-34978886 None:intergenic 50.0%
GCCATAGTAGTTACCGCGTT+GGG + chr4_1:34978916-34978935 Msa0545160:three_prime_UTR 50.0%
TCCCAACGCGGTAACTACTA+TGG - chr4_1:34978920-34978939 None:intergenic 50.0%
GGATTTAAGTGTAGGAGGGG+TGG - chr4_1:34979141-34979160 None:intergenic 50.0%
TGGCGCATCGGAGAAATTTG+AGG - chr4_1:34979162-34979181 None:intergenic 50.0%
GTGATTTCAAGCTGGCGCAT+CGG - chr4_1:34979174-34979193 None:intergenic 50.0%
ACCAAATTGCCGTCTCCCTT+GGG - chr4_1:34979456-34979475 None:intergenic 50.0%
AACCAAATTGCCGTCTCCCT+TGG - chr4_1:34979457-34979476 None:intergenic 50.0%
GGAGGACGTTGTTCAAGATG+AGG + chr4_1:34979514-34979533 Msa0545160:three_prime_UTR 50.0%
GGACATTCTGTGCCTTGGAA+TGG + chr4_1:34979731-34979750 Msa0545160:three_prime_UTR 50.0%
TGGAGGTAAAGGGCCAATGG+AGG - chr4_1:34976486-34976505 None:intergenic 55.0%
GCAACTTCTCCGATGAGAGG+CGG + chr4_1:34977309-34977328 Msa0545160:CDS 55.0%
GCTTCTCTACCGCCTCTCAT+CGG - chr4_1:34977321-34977340 None:intergenic 55.0%
AGAGGCAGTCGTCTAAAGGC+TGG + chr4_1:34977354-34977373 Msa0545160:CDS 55.0%
TCAGGGGATGCCTTTACAGG+TGG - chr4_1:34977483-34977502 None:intergenic 55.0%
AAGGGGATGGAGTTCCTCCT+AGG + chr4_1:34977867-34977886 Msa0545160:CDS 55.0%
CTCCTCCAAGCTTACTTCGG+CGG + chr4_1:34977903-34977922 Msa0545160:CDS 55.0%
!! GCGAATGATAGCGGGAAGCT+AGG + chr4_1:34977977-34977996 Msa0545160:CDS 55.0%
!! GTTGTCGACGGACTACTGTC+TGG - chr4_1:34978276-34978295 None:intergenic 55.0%
CAACGCGAACAGGTTGTCGA+CGG - chr4_1:34978288-34978307 None:intergenic 55.0%
AGCCATCGTACAACGCGAAC+AGG - chr4_1:34978298-34978317 None:intergenic 55.0%
CGCCATAGTAGTTACCGCGT+TGG + chr4_1:34978915-34978934 Msa0545160:three_prime_UTR 55.0%
TGGGAGTGATCAGCCGGTAA+TGG + chr4_1:34978935-34978954 Msa0545160:three_prime_UTR 55.0%
TAAGGTCTCCCCAAGGGAGA+CGG + chr4_1:34979444-34979463 Msa0545160:three_prime_UTR 55.0%
CCAAATTGCCGTCTCCCTTG+GGG - chr4_1:34979455-34979474 None:intergenic 55.0%
GGCAGTCGTCTAAAGGCTGG+TGG + chr4_1:34977357-34977376 Msa0545160:CDS 60.0%
GTAGGCCGCCGAAGTAAGCT+TGG - chr4_1:34977911-34977930 None:intergenic 60.0%
!! CTTCGGCGGCCTACTTTTCC+AGG + chr4_1:34977917-34977936 Msa0545160:CDS 60.0%
CAGGACGAATGAGGCTGGCA+GGG + chr4_1:34977936-34977955 Msa0545160:CDS 60.0%
CCCCAAGGGAGACGGCAATT+TGG + chr4_1:34979452-34979471 Msa0545160:three_prime_UTR 60.0%
GGCCGCCGAAGTAAGCTTGG+AGG - chr4_1:34977908-34977927 None:intergenic 65.0%
CCTGCCAGCCTCATTCGTCC+TGG - chr4_1:34977938-34977957 None:intergenic 65.0%
CCAGGACGAATGAGGCTGGC+AGG + chr4_1:34977935-34977954 Msa0545160:CDS 65.0%
CCGGCTGATCACTCCCAACG+CGG - chr4_1:34978932-34978951 None:intergenic 65.0%
CCGCGTTGGGAGTGATCAGC+CGG + chr4_1:34978929-34978948 Msa0545160:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 34975772 34979779 34975772 ID=Msa0545160;Name=Msa0545160
chr4_1 mRNA 34975772 34979779 34975772 ID=Msa0545160-mRNA-1;Parent=Msa0545160;Name=Msa0545160-mRNA-1;_AED=0.08;_eAED=0.10;_QI=0|0|0.14|1|0.5|0.42|7|1566|451
chr4_1 exon 34975772 34975814 34975772 ID=Msa0545160-mRNA-1:exon:8827;Parent=Msa0545160-mRNA-1
chr4_1 exon 34976055 34976162 34976055 ID=Msa0545160-mRNA-1:exon:8828;Parent=Msa0545160-mRNA-1
chr4_1 exon 34976246 34976295 34976246 ID=Msa0545160-mRNA-1:exon:8829;Parent=Msa0545160-mRNA-1
chr4_1 exon 34976449 34976564 34976449 ID=Msa0545160-mRNA-1:exon:8830;Parent=Msa0545160-mRNA-1
chr4_1 exon 34976775 34976826 34976775 ID=Msa0545160-mRNA-1:exon:8831;Parent=Msa0545160-mRNA-1
chr4_1 exon 34976925 34977485 34976925 ID=Msa0545160-mRNA-1:exon:8832;Parent=Msa0545160-mRNA-1
chr4_1 exon 34977788 34979779 34977788 ID=Msa0545160-mRNA-1:exon:8833;Parent=Msa0545160-mRNA-1
chr4_1 CDS 34975772 34975814 34975772 ID=Msa0545160-mRNA-1:cds;Parent=Msa0545160-mRNA-1
chr4_1 CDS 34976055 34976162 34976055 ID=Msa0545160-mRNA-1:cds;Parent=Msa0545160-mRNA-1
chr4_1 CDS 34976246 34976295 34976246 ID=Msa0545160-mRNA-1:cds;Parent=Msa0545160-mRNA-1
chr4_1 CDS 34976449 34976564 34976449 ID=Msa0545160-mRNA-1:cds;Parent=Msa0545160-mRNA-1
chr4_1 CDS 34976775 34976826 34976775 ID=Msa0545160-mRNA-1:cds;Parent=Msa0545160-mRNA-1
chr4_1 CDS 34976925 34977485 34976925 ID=Msa0545160-mRNA-1:cds;Parent=Msa0545160-mRNA-1
chr4_1 CDS 34977788 34978213 34977788 ID=Msa0545160-mRNA-1:cds;Parent=Msa0545160-mRNA-1
chr4_1 three_prime_UTR 34978214 34979779 34978214 ID=Msa0545160-mRNA-1:three_prime_utr;Parent=Msa0545160-mRNA-1
Gene Sequence

>Msa0545160

GTTCCAACATCTTTCTTACCAAAGGATCAAGATATTCGCCTCGTAATTGCTATAAATGTTTCTGTGCAGGTGGTAGGAACTTTGAATTACATGTGTCCTGAACTTCTTGCAGATATTCCGTATGGATTCAAATCTGATATTTGGTCATTAGGCTGCTGTGTATACGAGATGGCTGCTCATCGTCCAGCCTTTAAAGCATTTGATATGGCAGGATTGATAAGCAAGATAAACCGTTCCTCCATTGGCCCTTTACCTCCATGTTATTCCCCCTCGTTGCAAGTATCCATCTATCTGCTTTTGATATTACGATCAATACTGAAAATACTAATAAAAGGCATGTTGAGAAAAAGTCCCGAGCATCGACCAACAGCATCTGAGGTGTTGAAGCATCCTTATTTGCAACCTTACGTCGATCAATACCGTCCATCTTTTGCTCCTCTAACAGCCTTTTCTCCTGAAAAACCGATTTCATCTGTCACTTATACTAAAAGACATATGGCTGAAAGTCAGAACAGTACTAGTTCAAGTAGTGACAAAGACAGTTTAATGTCAAATGAGAGAAACATTGAAACAACAGCGCCAAAGTGTGATAACAAAGCCACTGAGATGGATATTGCATTGATCGATGATGATGGCTCTGAGCAAGAGGTGTTAAAGTCATCTCATAATGAACAGCATTCTAATGTTGAATCTAAACAGCCAAAAACAATTATAAATATAATGATGGCATTGAAAGAAGGGAAAGGTAAAGAAGCAACTTCTCCGATGAGAGGCGGTAGAGAAGCAACTTCTCCGATGAGAGGCAGTCGTCTAAAGGCTGGTGGAGTATCGGCGCCGAAAGTAAATACAGACATATCGTCTAAATTGCCTAAACCTACATTCATTGCTCCTAGTTTGAAGCCTAATTTAGAGTCACCGACTGTTCCACCTTTACTGATGACAGAGCCCTCACCAAAAGTTAAACCGAAACATGATATGACTCCACCATCTTCCTCTATCAAACAAGTAGAAGGGGATGGAGTTCCTCCTAGGCCAAGACAAAGAACTCCTCCAAGCTTACTTCGGCGGCCTACTTTTCCAGGACGAATGAGGCTGGCAGGGTTTGATGTTCCGAATGCAGCGAATGATAGCGGGAAGCTAGGTCCAAGTAATACAGGCCAGGAATCTGAGATGAGCCATTGTCATTTGTTCAATGGCAATGTACCTCGTATTTCTAGACCGATTACTAGAGAACCTCATAAGGCTTTTGAAAGAAATTCTAAAGCAATGCAAACAGAAAGTAGTAATTCTGCTTCGTCTTCGGTTTCTATTCAGGGATGTGAACTTGTTGATGATGCAACAACTTTTATGACATGA
Protein sequence

>Msa0545160

VPTSFLPKDQDIRLVIAINVSVQVVGTLNYMCPELLADIPYGFKSDIWSLGCCVYEMAAHRPAFKAFDMAGLISKINRSSIGPLPPCYSPSLQVSIYLLLILRSILKILIKGMLRKSPEHRPTASEVLKHPYLQPYVDQYRPSFAPLTAFSPEKPISSVTYTKRHMAESQNSTSSSSDKDSLMSNERNIETTAPKCDNKATEMDIALIDDDGSEQEVLKSSHNEQHSNVESKQPKTIINIMMALKEGKGKEATSPMRGGREATSPMRGSRLKAGGVSAPKVNTDISSKLPKPTFIAPSLKPNLESPTVPPLLMTEPSPKVKPKHDMTPPSSSIKQVEGDGVPPRPRQRTPPSLLRRPTFPGRMRLAGFDVPNAANDSGKLGPSNTGQESEMSHCHLFNGNVPRISRPITREPHKAFERNSKAMQTESSNSASSSVSIQGCELVDDATTFMT*