AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0545710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545710 MtrunA17_Chr4g0026521 91.864 295 20 2 1 291 1 295 3.87e-175 485
Msa0545710 MtrunA17_Chr2g0321291 67.314 309 74 8 5 291 6 309 1.29e-132 378
Msa0545710 MtrunA17_Chr2g0328921 74.242 198 43 2 73 263 91 287 2.00e-109 319
Msa0545710 MtrunA17_Chr1g0173841 63.025 238 61 5 73 286 150 384 3.51e-106 314
Msa0545710 MtrunA17_Chr7g0258011 55.797 276 87 5 43 286 73 345 6.85e-106 311
Msa0545710 MtrunA17_Chr8g0352011 64.159 226 66 3 73 286 113 335 4.01e-105 309
Msa0545710 MtrunA17_Chr5g0422571 70.157 191 51 2 73 262 65 250 4.55e-102 300
Msa0545710 MtrunA17_Chr1g0198651 78.710 155 30 1 111 262 99 253 4.75e-96 285
Msa0545710 MtrunA17_Chr4g0053341 59.130 230 80 4 38 262 2 222 7.63e-95 279
Msa0545710 MtrunA17_Chr1g0200421 38.415 164 89 6 121 276 245 404 1.85e-26 108
Msa0545710 MtrunA17_Chr2g0320341 34.132 167 100 6 114 273 231 394 2.02e-22 95.9
Msa0545710 MtrunA17_Chr8g0382121 32.105 190 120 7 78 262 78 263 2.47e-20 89.0
Msa0545710 MtrunA17_Chr4g0029521 37.410 139 78 4 121 258 61 191 2.63e-20 90.5
Msa0545710 MtrunA17_Chr1g0185121 31.771 192 119 6 69 258 12 193 3.32e-20 90.1
Msa0545710 MtrunA17_Chr2g0326381 33.333 159 95 4 114 268 48 199 5.32e-20 89.4
Msa0545710 MtrunA17_Chr1g0181381 34.932 146 86 4 114 258 54 191 6.44e-20 89.4
Msa0545710 MtrunA17_Chr1g0151351 35.461 141 82 4 119 258 62 194 2.31e-19 87.8
Msa0545710 MtrunA17_Chr3g0123941 34.426 183 103 9 119 289 130 307 2.51e-19 86.7
Msa0545710 MtrunA17_Chr7g0263941 36.054 147 82 6 114 258 50 186 3.67e-19 86.7
Msa0545710 MtrunA17_Chr8g0373541 32.558 172 98 7 96 258 23 185 6.73e-19 86.7
Msa0545710 MtrunA17_Chr7g0268961 35.714 140 78 6 121 258 45 174 6.95e-19 86.3
Msa0545710 MtrunA17_Chr8g0342421 30.000 180 114 5 81 258 45 214 1.72e-18 85.5
Msa0545710 MtrunA17_Chr1g0191991 35.714 140 78 5 121 258 86 215 1.77e-18 85.1
Msa0545710 MtrunA17_Chr2g0330171 31.707 164 100 5 97 258 45 198 4.29e-18 84.0
Msa0545710 MtrunA17_Chr1g0210821 34.014 147 85 5 114 258 60 196 6.45e-18 83.6
Msa0545710 MtrunA17_Chr1g0199101 34.014 147 85 6 114 258 63 199 1.01e-17 83.2
Msa0545710 MtrunA17_Chr3g0141431 33.129 163 91 5 104 256 20 174 1.13e-17 82.4
Msa0545710 MtrunA17_Chr1g0162971 35.000 140 79 5 121 258 64 193 2.60e-17 81.6
Msa0545710 MtrunA17_Chr2g0324991 34.507 142 83 5 114 253 8 141 5.24e-17 76.3
Msa0545710 MtrunA17_Chr1g0163001 33.333 147 86 5 114 258 59 195 8.04e-17 80.5
Msa0545710 MtrunA17_Chr4g0040571 31.507 146 90 4 114 258 55 191 5.15e-16 77.8
Msa0545710 MtrunA17_Chr8g0375521 30.935 139 85 5 119 254 48 178 1.36e-12 67.4
Msa0545710 MtrunA17_Chr6g0479921 27.607 163 106 5 121 281 52 204 4.45e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0545710 AT1G08290.1 53.636 330 92 7 9 282 12 336 8.49e-113 329
Msa0545710 AT3G57670.1 77.143 175 37 2 111 285 201 372 2.15e-102 304
Msa0545710 AT1G51220.1 52.817 284 102 5 11 262 30 313 4.41e-102 302
Msa0545710 AT3G20880.1 74.302 179 42 1 109 287 238 412 9.62e-102 303
Msa0545710 AT1G13290.1 70.312 192 47 4 73 262 55 238 5.31e-98 290
Msa0545710 AT1G34790.1 62.385 218 72 3 74 284 85 299 1.63e-94 281
Msa0545710 AT1G34370.3 37.931 174 91 7 121 285 28 193 4.54e-27 107
Msa0545710 AT1G34370.2 34.404 218 118 8 85 285 194 403 1.47e-26 108
Msa0545710 AT1G34370.1 34.404 218 118 8 85 285 194 403 1.47e-26 108
Msa0545710 AT5G22890.1 36.196 163 95 5 103 258 195 355 4.60e-24 100
Msa0545710 AT5G03150.1 35.714 140 79 5 121 258 76 206 3.67e-20 90.5
Msa0545710 AT5G66730.1 35.461 141 81 4 119 258 53 184 1.12e-19 89.4
Msa0545710 AT5G60470.1 36.691 139 79 4 121 258 58 188 4.06e-19 87.4
Msa0545710 AT1G55110.3 31.098 164 102 5 97 258 62 216 4.47e-19 87.0
Msa0545710 AT1G55110.1 31.098 164 102 5 97 258 62 216 4.47e-19 87.0
Msa0545710 AT1G55110.2 31.098 164 102 5 97 258 62 216 4.47e-19 87.0
Msa0545710 AT5G60470.3 36.691 139 79 4 121 258 63 193 5.89e-19 87.0
Msa0545710 AT5G60470.2 35.971 139 80 4 121 258 67 197 6.69e-19 86.7
Msa0545710 AT3G45260.2 35.971 139 80 4 121 258 62 192 2.39e-18 85.1
Msa0545710 AT3G45260.1 35.971 139 80 4 121 258 62 192 2.39e-18 85.1
Msa0545710 AT2G02080.1 30.864 162 102 4 98 258 54 206 3.75e-18 84.7
Msa0545710 AT2G02080.4 30.864 162 102 4 98 258 54 206 3.75e-18 84.7
Msa0545710 AT2G02080.5 30.864 162 102 4 98 258 54 206 3.75e-18 84.7
Msa0545710 AT2G02080.3 30.864 162 102 4 98 258 54 206 3.75e-18 84.7
Msa0545710 AT1G14580.1 31.902 163 99 5 98 258 53 205 3.86e-18 84.7
Msa0545710 AT1G14580.2 31.902 163 99 5 98 258 53 205 3.86e-18 84.7
Msa0545710 AT1G14580.3 31.902 163 99 5 98 258 67 219 3.97e-18 84.7
Msa0545710 AT2G02070.2 31.481 162 101 4 98 258 52 204 5.04e-18 84.3
Msa0545710 AT2G02070.1 31.481 162 101 4 98 258 52 204 5.04e-18 84.3
Msa0545710 AT5G44160.1 29.670 182 114 6 81 258 18 189 7.25e-18 83.6
Msa0545710 AT1G03840.1 32.515 163 100 5 97 258 40 193 9.34e-18 83.6
Msa0545710 AT3G13810.5 31.481 162 102 4 98 258 65 218 1.25e-17 83.2
Msa0545710 AT3G13810.4 31.481 162 102 4 98 258 70 223 1.31e-17 83.2
Msa0545710 AT3G13810.1 31.481 162 102 4 98 258 70 223 1.31e-17 83.2
Msa0545710 AT3G13810.6 31.481 162 102 4 98 258 51 204 1.34e-17 83.2
Msa0545710 AT2G02080.6 35.821 134 76 4 126 258 5 129 1.46e-17 82.8
Msa0545710 AT2G02080.2 35.821 134 76 4 126 258 5 129 1.46e-17 82.8
Msa0545710 AT3G13810.2 33.562 146 88 4 114 258 87 224 1.50e-17 82.8
Msa0545710 AT3G13810.3 33.562 146 88 4 114 258 73 210 1.59e-17 82.8
Msa0545710 AT3G50700.1 33.562 146 87 4 114 258 50 186 1.89e-17 82.4
Msa0545710 AT1G03840.2 34.247 146 86 5 114 258 55 191 2.05e-17 82.4
Msa0545710 AT5G44160.2 33.094 139 83 4 121 258 21 150 2.32e-17 82.0
Msa0545710 AT4G02670.2 34.247 146 87 4 114 258 71 208 2.63e-17 81.6
Msa0545710 AT4G02670.1 34.247 146 87 4 114 258 69 206 2.84e-17 81.6
Msa0545710 AT1G25250.1 28.986 138 90 4 119 254 31 162 1.93e-12 67.4
Msa0545710 AT1G68130.1 30.435 138 87 4 119 254 62 192 1.95e-12 67.4
Msa0545710 AT1G25250.3 28.986 138 90 4 119 254 54 185 3.14e-12 66.6
Msa0545710 AT2G01940.1 30.216 139 86 5 119 254 65 195 1.61e-11 64.7

Find 72 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAAAACTAATAATTAATT+TGG 0.072784 4_1:-36270766 None:intergenic
GTGAACTTTGATAAACTTTC+TGG 0.135682 4_1:+36274435 Msa0545710:three_prime_UTR
AAAGATCATATTAGGTCTTT+TGG 0.190747 4_1:+36273825 Msa0545710:CDS
GAAAACTTTGCACTCAAAAT+AGG 0.212077 4_1:-36270977 None:intergenic
AGAAAACCTTCCAGAGGTTT+TGG 0.230422 4_1:+36271155 Msa0545710:CDS
CCATGCTACTGTTGTGTTCA+AGG 0.271424 4_1:+36273585 Msa0545710:CDS
ACTCATCAAGGTAAAGAATA+TGG 0.309783 4_1:+36270820 Msa0545710:exon
GATCTCTCAAAGATCATATT+AGG 0.336377 4_1:+36273817 Msa0545710:CDS
GAGACTGTGGCTTTGCATAT+TGG 0.341519 4_1:+36271052 Msa0545710:CDS
ATAAAACTAATAATTAATTT+GGG 0.347784 4_1:-36270765 None:intergenic
CTTACATACAGAAATCCTTT+TGG 0.368821 4_1:-36274396 None:intergenic
CATGTTGTTATACCTATTGA+AGG 0.383480 4_1:-36271242 None:intergenic
AACTCAAAACCCTCAATTTG+TGG 0.388963 4_1:-36271022 None:intergenic
TCTTTCAAAGGCTTAGCTCT+TGG 0.403693 4_1:-36273630 None:intergenic
TATGAACTAGAGGAATTAGA+TGG 0.411662 4_1:-36270893 None:intergenic
TGTGTGCCTCTTAGTGAGTC+TGG 0.423624 4_1:-36273540 None:intergenic
AAAAGAATTGTGGCAAGTTA+TGG 0.431308 4_1:+36273760 Msa0545710:CDS
ACTCCTGCACAGATCCTTGT+TGG 0.433190 4_1:+36271184 Msa0545710:CDS
TATGCAACAAAACCTTCAAT+AGG 0.434246 4_1:+36271230 Msa0545710:CDS
GGTAAAGAATATGGCAATGA+TGG 0.440587 4_1:+36270829 Msa0545710:exon
TTTGCTACGGAGCACTTATA+GGG 0.444748 4_1:+36274197 Msa0545710:three_prime_UTR
GATTGATGATCAAGCTTTCG+AGG 0.449639 4_1:+36273872 Msa0545710:CDS
ACGTCAAGTGCCACAAATTG+AGG 0.451497 4_1:+36271012 Msa0545710:CDS
CATTTGCTGTGAAAGGTGAT+TGG 0.459235 4_1:+36273727 Msa0545710:CDS
GAAACCAAGCACTTATATGA+TGG 0.481094 4_1:-36270797 None:intergenic
TCTGGTATGTAAATCATTGT+CGG 0.482496 4_1:+36274453 Msa0545710:three_prime_UTR
CCATTTATGTGTAGAAAATG+TGG 0.488125 4_1:+36273699 Msa0545710:CDS
GGTAGCCTTAGCATTGCAGC+TGG 0.491825 4_1:-36273564 None:intergenic
TTCATCTACTTCACCACTTG+AGG 0.496683 4_1:-36274282 None:intergenic
CAAACCATCATATAAGTGCT+TGG 0.500864 4_1:+36270793 Msa0545710:five_prime_UTR
ATCGGCTATCATATCTATAG+AGG 0.506255 4_1:+36274225 Msa0545710:three_prime_UTR
ATAAATATAGTTACCTCAAG+TGG 0.511554 4_1:+36274269 Msa0545710:three_prime_UTR
AAGTTATGGTATTGCACTTG+TGG 0.518903 4_1:+36273774 Msa0545710:CDS
GTGCTTGGTTTCACTCATCA+AGG 0.520374 4_1:+36270808 Msa0545710:five_prime_UTR
TTTCTTCTTCTTCTTTAACA+TGG 0.521689 4_1:-36271123 None:intergenic
TTTCCATTAGATGCATATGT+GGG 0.526101 4_1:+36273491 Msa0545710:intron
TTATCCGCTTCATTACCAAA+AGG 0.529018 4_1:+36274381 Msa0545710:three_prime_UTR
CGTCAAGTGCCACAAATTGA+GGG 0.531074 4_1:+36271013 Msa0545710:CDS
CACAACCAGCTGCAATGCTA+AGG 0.533499 4_1:+36273559 Msa0545710:CDS
AGAGTTCTAAAATCTTTCAA+AGG 0.533941 4_1:-36273642 None:intergenic
TCTATAAGTTTCAATAACAA+AGG 0.539223 4_1:-36273980 None:intergenic
GAGTTGGTATCCAAAACCTC+TGG 0.540847 4_1:-36271165 None:intergenic
TCAAGCTTTCGAGGAGGAGA+AGG 0.557211 4_1:+36273881 Msa0545710:CDS
ACAAGGATCTGTGCAGGAGT+TGG 0.561642 4_1:-36271181 None:intergenic
GGTCCAACAAGGATCTGTGC+AGG 0.564499 4_1:-36271187 None:intergenic
AGGACAATAATTTCATTGAG+TGG 0.566958 4_1:+36270864 Msa0545710:CDS
TCCATTAGATGCATATGTGG+GGG 0.571799 4_1:+36273493 Msa0545710:intron
AAAGGACCAGACTCACTAAG+AGG 0.575770 4_1:+36273534 Msa0545710:CDS
TTCTCATAGTAATAGAGGGT+AGG 0.583747 4_1:+36274310 Msa0545710:three_prime_UTR
ATATTGCTAACGCATGAACA+TGG 0.587811 4_1:+36274168 Msa0545710:three_prime_UTR
TTGCTGTGAAAGGTGATTGG+AGG 0.589362 4_1:+36273730 Msa0545710:CDS
TGAGTGGTGGAAGAAGAAGA+TGG 0.593836 4_1:-36270932 None:intergenic
CTTCTAAGAAAACCTTCCAG+AGG 0.598395 4_1:+36271149 Msa0545710:CDS
TTCCATTAGATGCATATGTG+GGG 0.605042 4_1:+36273492 Msa0545710:intron
TAGTGAATAGTGATTAAACT+TGG 0.606754 4_1:-36274113 None:intergenic
CATTTATGTGTAGAAAATGT+GGG 0.610048 4_1:+36273700 Msa0545710:CDS
ACTCACTACAAAAGAAAACA+TGG 0.615968 4_1:+36273669 Msa0545710:CDS
AAAGCTTGATCATCAATCGA+AGG 0.617239 4_1:-36273867 None:intergenic
CAATAGGTATAACAACATGC+AGG 0.621278 4_1:+36271246 Msa0545710:CDS
TATTGCTAACGCATGAACAT+GGG 0.623074 4_1:+36274169 Msa0545710:three_prime_UTR
AGGACTCATGAAAAGAATTG+TGG 0.623632 4_1:+36273750 Msa0545710:CDS
GGGAAAACATTTGCTGTGAA+AGG 0.623807 4_1:+36273720 Msa0545710:CDS
TGAAGTTAGATATGAACTAG+AGG 0.628204 4_1:-36270903 None:intergenic
TGGTATCCAAAACCTCTGGA+AGG 0.630382 4_1:-36271161 None:intergenic
CATGGATCTGAATTTAGGAA+AGG 0.631532 4_1:+36273516 Msa0545710:CDS
AGCACTTATAGGGTAGTGAT+CGG 0.634722 4_1:+36274207 Msa0545710:three_prime_UTR
TGAGTTCAAAGAGGAGACTG+TGG 0.640242 4_1:+36271039 Msa0545710:CDS
ATGGAGCAAGAAAATTGCAT+AGG 0.642845 4_1:-36271208 None:intergenic
AAAATTGCATAGGTCCAACA+AGG 0.669488 4_1:-36271198 None:intergenic
CCTTGAACACAACAGTAGCA+TGG 0.671135 4_1:-36273585 None:intergenic
AATGATGGAGAGTGATGTAG+AGG 0.688074 4_1:+36270844 Msa0545710:CDS
TGATGATCAAGCTTTCGAGG+AGG 0.694755 4_1:+36273875 Msa0545710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGAATATAAAAACTATA+AGG + chr4_1:36271539-36271558 Msa0545710:intron 10.0%
!!! AACTGATTTTTTTAAATAAA+AGG - chr4_1:36271711-36271730 None:intergenic 10.0%
!! ATAACAAAAAAAAATATAGA+TGG + chr4_1:36271857-36271876 Msa0545710:intron 10.0%
!! TAACAAAAAAAAATATAGAT+GGG + chr4_1:36271858-36271877 Msa0545710:intron 10.0%
!! ATATAAATATAAATAAATGG+AGG + chr4_1:36272845-36272864 Msa0545710:intron 10.0%
!! AATTAATTAGTATAATCAAA+AGG - chr4_1:36272962-36272981 None:intergenic 10.0%
!!! ATGATAATTTATCTTTTTTT+AGG - chr4_1:36273234-36273253 None:intergenic 10.0%
!!! GTTTCTTTTATATTTTATTT+TGG - chr4_1:36273380-36273399 None:intergenic 10.0%
!! AAAATATTTATTAATTGAGT+TGG - chr4_1:36273473-36273492 None:intergenic 10.0%
!!! TATAGTTTTCCATTTTTTTA+GGG - chr4_1:36271381-36271400 None:intergenic 15.0%
!!! TTATAGTTTTCCATTTTTTT+AGG - chr4_1:36271382-36271401 None:intergenic 15.0%
!!! AAGGCAAATTTTAATTATAA+GGG + chr4_1:36271648-36271667 Msa0545710:intron 15.0%
!!! ATTTTAATTATAAGGGATTT+TGG + chr4_1:36271655-36271674 Msa0545710:intron 15.0%
!!! ATTTGAAGTAAACAAATTAA+GGG - chr4_1:36272170-36272189 None:intergenic 15.0%
!!! AATTTGAAGTAAACAAATTA+AGG - chr4_1:36272171-36272190 None:intergenic 15.0%
!!! GGAAACATTTTTTTTTTTTT+TGG - chr4_1:36272246-36272265 None:intergenic 15.0%
!!! TATTCTCCTTTTTTTTTTAT+AGG + chr4_1:36272299-36272318 Msa0545710:intron 15.0%
!! AAATAGCCTATAAAAAAAAA+AGG - chr4_1:36272308-36272327 None:intergenic 15.0%
!!! TCAAAAAGTTAATTTTTCAT+CGG + chr4_1:36272473-36272492 Msa0545710:intron 15.0%
!! TGAAAGATTAACAAAATTAT+TGG + chr4_1:36273093-36273112 Msa0545710:intron 15.0%
!! CAGTTACTTATAAAGAAAAA+TGG + chr4_1:36271347-36271366 Msa0545710:intron 20.0%
!!! ATAGTTTTCCATTTTTTTAG+GGG - chr4_1:36271380-36271399 None:intergenic 20.0%
!!! CAAGGCAAATTTTAATTATA+AGG + chr4_1:36271647-36271666 Msa0545710:intron 20.0%
!!! ATTAACTTTTTGAAAGTGAA+GGG - chr4_1:36272466-36272485 None:intergenic 20.0%
!!! AATTAACTTTTTGAAAGTGA+AGG - chr4_1:36272467-36272486 None:intergenic 20.0%
!!! ACGTTTTTAATACTTAGTAA+TGG - chr4_1:36272710-36272729 None:intergenic 20.0%
!!! TGAAGATCAATTTTATTTCT+TGG + chr4_1:36272736-36272755 Msa0545710:intron 20.0%
!! AAGAAAACAAAACAAAACTT+TGG + chr4_1:36272931-36272950 Msa0545710:intron 20.0%
!!! TTGTTTTATTTCAACATTCA+TGG + chr4_1:36273019-36273038 Msa0545710:intron 20.0%
!! AAAATTATTGGCAACAATTA+TGG + chr4_1:36273105-36273124 Msa0545710:intron 20.0%
!!! TTATGGTCTAATTTAAAGTA+AGG + chr4_1:36273184-36273203 Msa0545710:intron 20.0%
!!! TATGGTCTAATTTAAAGTAA+GGG + chr4_1:36273185-36273204 Msa0545710:intron 20.0%
!! TCTATAAGTTTCAATAACAA+AGG - chr4_1:36273983-36274002 None:intergenic 20.0%
!! ACTTATAGAAAAAGTTGAAA+AGG + chr4_1:36273994-36274013 Msa0545710:three_prime_UTR 20.0%
!!! TTTTTTTCTCATAGTAATAG+AGG + chr4_1:36274305-36274324 Msa0545710:three_prime_UTR 20.0%
!!! TTTTTTCTCATAGTAATAGA+GGG + chr4_1:36274306-36274325 Msa0545710:three_prime_UTR 20.0%
!! TTGTATAGTTTCATTTTGAG+TGG - chr4_1:36270951-36270970 None:intergenic 25.0%
!!! ATCATCAAGATATGTTTTAG+TGG - chr4_1:36271089-36271108 None:intergenic 25.0%
! TTTCTTCTTCTTCTTTAACA+TGG - chr4_1:36271126-36271145 None:intergenic 25.0%
! TTCGTAAGTAAATTTAGTGA+GGG - chr4_1:36271461-36271480 None:intergenic 25.0%
! ATTCGTAAGTAAATTTAGTG+AGG - chr4_1:36271462-36271481 None:intergenic 25.0%
! CAAAAAAAAATATAGATGGG+AGG + chr4_1:36271861-36271880 Msa0545710:intron 25.0%
!!! TATTTGTGTTAGACCTTTTT+TGG + chr4_1:36272551-36272570 Msa0545710:intron 25.0%
!!! ATTTGTGTTAGACCTTTTTT+GGG + chr4_1:36272552-36272571 Msa0545710:intron 25.0%
!! GTTAATTTTCAACCCAAAAA+AGG - chr4_1:36272567-36272586 None:intergenic 25.0%
! ACAAAACAGACAATATTCTA+GGG - chr4_1:36272590-36272609 None:intergenic 25.0%
! TATAAATAAATGGAGGACAT+TGG + chr4_1:36272852-36272871 Msa0545710:intron 25.0%
! ATAAATAAATGGAGGACATT+GGG + chr4_1:36272853-36272872 Msa0545710:intron 25.0%
!! ATAGTAATTTGTCAAAAGGA+GGG + chr4_1:36272878-36272897 Msa0545710:intron 25.0%
! TTTGTTAATCTTTCACTGAT+GGG - chr4_1:36273088-36273107 None:intergenic 25.0%
!! TTTTGTTAATCTTTCACTGA+TGG - chr4_1:36273089-36273108 None:intergenic 25.0%
! AATCAAAGATGACATGAATT+AGG + chr4_1:36273341-36273360 Msa0545710:intron 25.0%
!!! AGAGTTCTAAAATCTTTCAA+AGG - chr4_1:36273645-36273664 None:intergenic 25.0%
! CATTTATGTGTAGAAAATGT+GGG + chr4_1:36273700-36273719 Msa0545710:CDS 25.0%
!! AAAGATCATATTAGGTCTTT+TGG + chr4_1:36273825-36273844 Msa0545710:CDS 25.0%
! TAGTGAATAGTGATTAAACT+TGG - chr4_1:36274116-36274135 None:intergenic 25.0%
! ATAAATATAGTTACCTCAAG+TGG + chr4_1:36274269-36274288 Msa0545710:three_prime_UTR 25.0%
!!! TTTTTTTTATCCCTCTTCAT+TGG + chr4_1:36274351-36274370 Msa0545710:three_prime_UTR 25.0%
!! TAAATTTTCTCCAATGAAGA+GGG - chr4_1:36274364-36274383 None:intergenic 25.0%
!! ATAAATTTTCTCCAATGAAG+AGG - chr4_1:36274365-36274384 None:intergenic 25.0%
ACTCATCAAGGTAAAGAATA+TGG + chr4_1:36270820-36270839 Msa0545710:exon 30.0%
AGGACAATAATTTCATTGAG+TGG + chr4_1:36270864-36270883 Msa0545710:CDS 30.0%
TATGAACTAGAGGAATTAGA+TGG - chr4_1:36270896-36270915 None:intergenic 30.0%
TGAAGTTAGATATGAACTAG+AGG - chr4_1:36270906-36270925 None:intergenic 30.0%
!! TATAGTTTCATTTTGAGTGG+TGG - chr4_1:36270948-36270967 None:intergenic 30.0%
GAAAACTTTGCACTCAAAAT+AGG - chr4_1:36270980-36270999 None:intergenic 30.0%
!!! ATTGAAGGTTTTGTTGCATA+TGG - chr4_1:36271230-36271249 None:intergenic 30.0%
TATGCAACAAAACCTTCAAT+AGG + chr4_1:36271230-36271249 Msa0545710:CDS 30.0%
CATGTTGTTATACCTATTGA+AGG - chr4_1:36271245-36271264 None:intergenic 30.0%
GAAAAACTCCCCTAAAAAAA+TGG + chr4_1:36271369-36271388 Msa0545710:intron 30.0%
TAAAAACTATAAGGTCTAGC+AGG + chr4_1:36271548-36271567 Msa0545710:intron 30.0%
!!! AAGTTCCTTCATTTTTTGAG+TGG + chr4_1:36271624-36271643 Msa0545710:intron 30.0%
!!! TAAAAGGAGCATCAGTTTTT+AGG + chr4_1:36272099-36272118 Msa0545710:intron 30.0%
ATAGATTAGCACCTACATTT+AGG + chr4_1:36272194-36272213 Msa0545710:intron 30.0%
CTTTGTGTTTGTCAAATTCA+TGG - chr4_1:36272267-36272286 None:intergenic 30.0%
AAAGGAGAATATCAAATTGC+TGG - chr4_1:36272290-36272309 None:intergenic 30.0%
AGACAAGAAGAGAAAAGTTA+GGG - chr4_1:36272379-36272398 None:intergenic 30.0%
AAGACAAGAAGAGAAAAGTT+AGG - chr4_1:36272380-36272399 None:intergenic 30.0%
ATCGGTTATGATCATTTCAA+AGG + chr4_1:36272491-36272510 Msa0545710:intron 30.0%
TCGGTTATGATCATTTCAAA+GGG + chr4_1:36272492-36272511 Msa0545710:intron 30.0%
CAAAACAGACAATATTCTAG+GGG - chr4_1:36272589-36272608 None:intergenic 30.0%
CACAAAACAGACAATATTCT+AGG - chr4_1:36272591-36272610 None:intergenic 30.0%
! ATATTGTCTGTTTTGTGTGT+TGG + chr4_1:36272594-36272613 Msa0545710:intron 30.0%
!!! TTTTGTGTGTTGGTTAGTTA+AGG + chr4_1:36272604-36272623 Msa0545710:intron 30.0%
!! TTTGTGTGTTGGTTAGTTAA+GGG + chr4_1:36272605-36272624 Msa0545710:intron 30.0%
TAAATAAATGGAGGACATTG+GGG + chr4_1:36272854-36272873 Msa0545710:intron 30.0%
! CATAGTAATTTGTCAAAAGG+AGG + chr4_1:36272877-36272896 Msa0545710:intron 30.0%
TTGTTAATCTTTCACTGATG+GGG - chr4_1:36273087-36273106 None:intergenic 30.0%
ACAATTATGGTCATATAGCT+AGG + chr4_1:36273118-36273137 Msa0545710:intron 30.0%
TACAACAATTTCTTCTCGTA+AGG + chr4_1:36273280-36273299 Msa0545710:intron 30.0%
TTTCTTCTCGTAAGGTTATA+TGG + chr4_1:36273288-36273307 Msa0545710:intron 30.0%
! TTTTCCATTAGATGCATATG+TGG + chr4_1:36273490-36273509 Msa0545710:intron 30.0%
TTTCCATTAGATGCATATGT+GGG + chr4_1:36273491-36273510 Msa0545710:intron 30.0%
! GTTTTCTTTTGTAGTGAGTT+TGG - chr4_1:36273668-36273687 None:intergenic 30.0%
ACTCACTACAAAAGAAAACA+TGG + chr4_1:36273669-36273688 Msa0545710:CDS 30.0%
! CCACATTTTCTACACATAAA+TGG - chr4_1:36273702-36273721 None:intergenic 30.0%
CCATTTATGTGTAGAAAATG+TGG + chr4_1:36273699-36273718 Msa0545710:CDS 30.0%
AAAAGAATTGTGGCAAGTTA+TGG + chr4_1:36273760-36273779 Msa0545710:CDS 30.0%
GATCTCTCAAAGATCATATT+AGG + chr4_1:36273817-36273836 Msa0545710:CDS 30.0%
!! CATATTAGGTCTTTTGGAAA+AGG + chr4_1:36273831-36273850 Msa0545710:CDS 30.0%
!!! AAATCCTTTTGGTAATGAAG+CGG - chr4_1:36274388-36274407 None:intergenic 30.0%
! CTTACATACAGAAATCCTTT+TGG - chr4_1:36274399-36274418 None:intergenic 30.0%
GTGAACTTTGATAAACTTTC+TGG + chr4_1:36274435-36274454 Msa0545710:three_prime_UTR 30.0%
CAAACCATCATATAAGTGCT+TGG + chr4_1:36270793-36270812 Msa0545710:five_prime_UTR 35.0%
GAAACCAAGCACTTATATGA+TGG - chr4_1:36270800-36270819 None:intergenic 35.0%
GGTAAAGAATATGGCAATGA+TGG + chr4_1:36270829-36270848 Msa0545710:exon 35.0%
AACTCAAAACCCTCAATTTG+TGG - chr4_1:36271025-36271044 None:intergenic 35.0%
!!! AGATATGTTTTAGTGGCATG+AGG - chr4_1:36271082-36271101 None:intergenic 35.0%
AAAATTGCATAGGTCCAACA+AGG - chr4_1:36271201-36271220 None:intergenic 35.0%
ATGGAGCAAGAAAATTGCAT+AGG - chr4_1:36271211-36271230 None:intergenic 35.0%
CAATAGGTATAACAACATGC+AGG + chr4_1:36271246-36271265 Msa0545710:CDS 35.0%
TATGGTGATCTCTCGTTATT+GGG - chr4_1:36271908-36271927 None:intergenic 35.0%
TTATGGTGATCTCTCGTTAT+TGG - chr4_1:36271909-36271928 None:intergenic 35.0%
AAGAAGAGAAAAGTTAGGGA+AGG - chr4_1:36272375-36272394 None:intergenic 35.0%
CTAAAAATAGAGCTAGCAAC+AGG + chr4_1:36272652-36272671 Msa0545710:intron 35.0%
! GGGCATAGTAATTTGTCAAA+AGG + chr4_1:36272874-36272893 Msa0545710:intron 35.0%
! TAATTTGTCAAAAGGAGGGT+AGG + chr4_1:36272882-36272901 Msa0545710:intron 35.0%
TTCCATTAGATGCATATGTG+GGG + chr4_1:36273492-36273511 Msa0545710:intron 35.0%
CATGGATCTGAATTTAGGAA+AGG + chr4_1:36273516-36273535 Msa0545710:CDS 35.0%
AGGACTCATGAAAAGAATTG+TGG + chr4_1:36273750-36273769 Msa0545710:CDS 35.0%
AAGTTATGGTATTGCACTTG+TGG + chr4_1:36273774-36273793 Msa0545710:CDS 35.0%
AAAGCTTGATCATCAATCGA+AGG - chr4_1:36273870-36273889 None:intergenic 35.0%
ATATTGCTAACGCATGAACA+TGG + chr4_1:36274168-36274187 Msa0545710:three_prime_UTR 35.0%
TATTGCTAACGCATGAACAT+GGG + chr4_1:36274169-36274188 Msa0545710:three_prime_UTR 35.0%
! AACATGGGAATGTTTTGCTA+CGG + chr4_1:36274184-36274203 Msa0545710:three_prime_UTR 35.0%
ATCGGCTATCATATCTATAG+AGG + chr4_1:36274225-36274244 Msa0545710:three_prime_UTR 35.0%
TTCTCATAGTAATAGAGGGT+AGG + chr4_1:36274310-36274329 Msa0545710:three_prime_UTR 35.0%
TTATCCGCTTCATTACCAAA+AGG + chr4_1:36274381-36274400 Msa0545710:three_prime_UTR 35.0%
! AATGATGGAGAGTGATGTAG+AGG + chr4_1:36270844-36270863 Msa0545710:CDS 40.0%
!!! TGAGGGTTTTGAGTTCAAAG+AGG + chr4_1:36271030-36271049 Msa0545710:CDS 40.0%
!! CTCTGGAAGGTTTTCTTAGA+AGG - chr4_1:36271151-36271170 None:intergenic 40.0%
! CTTCTAAGAAAACCTTCCAG+AGG + chr4_1:36271149-36271168 Msa0545710:CDS 40.0%
! AGAAAACCTTCCAGAGGTTT+TGG + chr4_1:36271155-36271174 Msa0545710:CDS 40.0%
CCTTGCCACTCAAAAAATGA+AGG - chr4_1:36271632-36271651 None:intergenic 40.0%
!!! CCTTCATTTTTTGAGTGGCA+AGG + chr4_1:36271629-36271648 Msa0545710:intron 40.0%
! CTCGTTATTGGGACTCTTTT+GGG - chr4_1:36271897-36271916 None:intergenic 40.0%
! TCTCGTTATTGGGACTCTTT+TGG - chr4_1:36271898-36271917 None:intergenic 40.0%
! TTTAGAGGCGGTGTTTGTTA+TGG - chr4_1:36271926-36271945 None:intergenic 40.0%
TTCTCTCCATCTATCTCTCT+GGG - chr4_1:36271961-36271980 None:intergenic 40.0%
AGAGATAGATGGAGAGAAGT+CGG + chr4_1:36271963-36271982 Msa0545710:intron 40.0%
TCAAACTGATAGGGCATACA+GGG - chr4_1:36272051-36272070 None:intergenic 40.0%
! CTGATGCTCCTTTTATGAGT+GGG - chr4_1:36272094-36272113 None:intergenic 40.0%
! ACTGATGCTCCTTTTATGAG+TGG - chr4_1:36272095-36272114 None:intergenic 40.0%
TTCCAAGACGAGAACTTCAT+TGG + chr4_1:36272141-36272160 Msa0545710:intron 40.0%
AGCACCTACATTTAGGCATA+CGG + chr4_1:36272201-36272220 Msa0545710:intron 40.0%
TTGTCCGTATGCCTAAATGT+AGG - chr4_1:36272208-36272227 None:intergenic 40.0%
AAAGTGAAGGGAATGCTAGA+GGG - chr4_1:36272454-36272473 None:intergenic 40.0%
TTTCTACTTAACTGCGAGAG+AGG + chr4_1:36272787-36272806 Msa0545710:intron 40.0%
TTCTACTTAACTGCGAGAGA+GGG + chr4_1:36272788-36272807 Msa0545710:intron 40.0%
TAATCTTTCACTGATGGGGT+AGG - chr4_1:36273083-36273102 None:intergenic 40.0%
TCCATTAGATGCATATGTGG+GGG + chr4_1:36273493-36273512 Msa0545710:intron 40.0%
! TCTTTCAAAGGCTTAGCTCT+TGG - chr4_1:36273633-36273652 None:intergenic 40.0%
!! GGGAAAACATTTGCTGTGAA+AGG + chr4_1:36273720-36273739 Msa0545710:CDS 40.0%
!! CATTTGCTGTGAAAGGTGAT+TGG + chr4_1:36273727-36273746 Msa0545710:CDS 40.0%
! GATTGATGATCAAGCTTTCG+AGG + chr4_1:36273872-36273891 Msa0545710:CDS 40.0%
!! TTTTGCTACGGAGCACTTAT+AGG + chr4_1:36274196-36274215 Msa0545710:three_prime_UTR 40.0%
! TTTGCTACGGAGCACTTATA+GGG + chr4_1:36274197-36274216 Msa0545710:three_prime_UTR 40.0%
AGCACTTATAGGGTAGTGAT+CGG + chr4_1:36274207-36274226 Msa0545710:three_prime_UTR 40.0%
TTCATCTACTTCACCACTTG+AGG - chr4_1:36274285-36274304 None:intergenic 40.0%
GTGCTTGGTTTCACTCATCA+AGG + chr4_1:36270808-36270827 Msa0545710:five_prime_UTR 45.0%
! TGAGTGGTGGAAGAAGAAGA+TGG - chr4_1:36270935-36270954 None:intergenic 45.0%
ACGTCAAGTGCCACAAATTG+AGG + chr4_1:36271012-36271031 Msa0545710:CDS 45.0%
CGTCAAGTGCCACAAATTGA+GGG + chr4_1:36271013-36271032 Msa0545710:CDS 45.0%
TGAGTTCAAAGAGGAGACTG+TGG + chr4_1:36271039-36271058 Msa0545710:CDS 45.0%
! GAGACTGTGGCTTTGCATAT+TGG + chr4_1:36271052-36271071 Msa0545710:CDS 45.0%
TGGTATCCAAAACCTCTGGA+AGG - chr4_1:36271164-36271183 None:intergenic 45.0%
!! GAGTTGGTATCCAAAACCTC+TGG - chr4_1:36271168-36271187 None:intergenic 45.0%
CTATAAGGTCTAGCAGGAGA+AGG + chr4_1:36271554-36271573 Msa0545710:intron 45.0%
!!! TGGGTTGGTGATTTTAGAGG+CGG - chr4_1:36271938-36271957 None:intergenic 45.0%
CTTCTCTCCATCTATCTCTC+TGG - chr4_1:36271962-36271981 None:intergenic 45.0%
ATAGATGGAGAGAAGTCGGA+TGG + chr4_1:36271967-36271986 Msa0545710:intron 45.0%
CTCAAACTGATAGGGCATAC+AGG - chr4_1:36272052-36272071 None:intergenic 45.0%
ATGCCCTATCAGTTTGAGCA+CGG + chr4_1:36272054-36272073 Msa0545710:intron 45.0%
ATCCCGTGCTCAAACTGATA+GGG - chr4_1:36272060-36272079 None:intergenic 45.0%
TATCCCGTGCTCAAACTGAT+AGG - chr4_1:36272061-36272080 None:intergenic 45.0%
TATCAGTTTGAGCACGGGAT+AGG + chr4_1:36272060-36272079 Msa0545710:intron 45.0%
TTGCACCTCCCACTCATAAA+AGG + chr4_1:36272083-36272102 Msa0545710:intron 45.0%
! ATGCTCCTTTTATGAGTGGG+AGG - chr4_1:36272091-36272110 None:intergenic 45.0%
TCGTCTTGGAAACGACTCAT+TGG - chr4_1:36272132-36272151 None:intergenic 45.0%
TGCCAATGAAGTTCTCGTCT+TGG - chr4_1:36272146-36272165 None:intergenic 45.0%
TCGAGCTAGCTATAGCTAGA+TGG + chr4_1:36272418-36272437 Msa0545710:intron 45.0%
GAAAGTGAAGGGAATGCTAG+AGG - chr4_1:36272455-36272474 None:intergenic 45.0%
GTGTAGGACATGTAACATCG+AGG + chr4_1:36272904-36272923 Msa0545710:intron 45.0%
AAAGGACCAGACTCACTAAG+AGG + chr4_1:36273534-36273553 Msa0545710:CDS 45.0%
CCTTGAACACAACAGTAGCA+TGG - chr4_1:36273588-36273607 None:intergenic 45.0%
CCATGCTACTGTTGTGTTCA+AGG + chr4_1:36273585-36273604 Msa0545710:CDS 45.0%
TTGCTGTGAAAGGTGATTGG+AGG + chr4_1:36273730-36273749 Msa0545710:CDS 45.0%
TGATGATCAAGCTTTCGAGG+AGG + chr4_1:36273875-36273894 Msa0545710:CDS 45.0%
!! ATAAAACTAATAATTAATTT+GGG - chr4_1:36270768-36270787 None:intergenic 5.0%
!! AATAAAACTAATAATTAATT+TGG - chr4_1:36270769-36270788 None:intergenic 5.0%
!!! TATATTTTTTTTTGTTATAT+CGG - chr4_1:36271855-36271874 None:intergenic 5.0%
!! ACTATATAAATATAAATAAA+TGG + chr4_1:36272842-36272861 Msa0545710:intron 5.0%
!!! GATTATTATTTATTTTTTTA+TGG + chr4_1:36273167-36273186 Msa0545710:intron 5.0%
!!! TTTCTTTTATATTTTATTTT+GGG - chr4_1:36273379-36273398 None:intergenic 5.0%
ACAAGGATCTGTGCAGGAGT+TGG - chr4_1:36271184-36271203 None:intergenic 50.0%
ACTCCTGCACAGATCCTTGT+TGG + chr4_1:36271184-36271203 Msa0545710:CDS 50.0%
!!! GGGTGGGTTGGTGATTTTAG+AGG - chr4_1:36271941-36271960 None:intergenic 50.0%
ATCTATCTCTCTGGGTGGGT+TGG - chr4_1:36271953-36271972 None:intergenic 50.0%
AACCCACCCAGAGAGATAGA+TGG + chr4_1:36271952-36271971 Msa0545710:intron 50.0%
CTCCATCTATCTCTCTGGGT+GGG - chr4_1:36271957-36271976 None:intergenic 50.0%
TCTCCATCTATCTCTCTGGG+TGG - chr4_1:36271958-36271977 None:intergenic 50.0%
ATGGAGAGAAGTCGGATGGA+GGG + chr4_1:36271971-36271990 Msa0545710:intron 50.0%
GCATACAGGGTTGTTGGAGA+TGG - chr4_1:36272038-36272057 None:intergenic 50.0%
GATAGGGCATACAGGGTTGT+TGG - chr4_1:36272044-36272063 None:intergenic 50.0%
TGCCCTATCAGTTTGAGCAC+GGG + chr4_1:36272055-36272074 Msa0545710:intron 50.0%
GAATGCTAGAGGGACACAAG+AGG - chr4_1:36272444-36272463 None:intergenic 50.0%
GTCAAAAGGAGGGTAGGTGT+AGG + chr4_1:36272888-36272907 Msa0545710:intron 50.0%
GCCCCCACATATGCATCTAA+TGG - chr4_1:36273497-36273516 None:intergenic 50.0%
TAGATGCATATGTGGGGGCA+TGG + chr4_1:36273498-36273517 Msa0545710:intron 50.0%
! GGGGGCATGGATCTGAATTT+AGG + chr4_1:36273511-36273530 Msa0545710:CDS 50.0%
TGTGTGCCTCTTAGTGAGTC+TGG - chr4_1:36273543-36273562 None:intergenic 50.0%
CACAACCAGCTGCAATGCTA+AGG + chr4_1:36273559-36273578 Msa0545710:CDS 50.0%
TCAAGCTTTCGAGGAGGAGA+AGG + chr4_1:36273881-36273900 Msa0545710:CDS 50.0%
GGTCCAACAAGGATCTGTGC+AGG - chr4_1:36271190-36271209 None:intergenic 55.0%
GATGGAGAGAAGTCGGATGG+AGG + chr4_1:36271970-36271989 Msa0545710:intron 55.0%
TGGAGAGAAGTCGGATGGAG+GGG + chr4_1:36271972-36271991 Msa0545710:intron 55.0%
GAGCAGCCATAAGACCAACC+AGG + chr4_1:36272002-36272021 Msa0545710:intron 55.0%
!! CGATCGCCTGGTTGGTCTTA+TGG - chr4_1:36272011-36272030 None:intergenic 55.0%
ATGGATCACGATCGCCTGGT+TGG - chr4_1:36272019-36272038 None:intergenic 55.0%
! GGTAGCCTTAGCATTGCAGC+TGG - chr4_1:36273567-36273586 None:intergenic 55.0%
GGAGATGGATCACGATCGCC+TGG - chr4_1:36272023-36272042 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 36270728 36274475 36270728 ID=Msa0545710;Name=Msa0545710
chr4_1 mRNA 36270728 36274475 36270728 ID=Msa0545710-mRNA-1;Parent=Msa0545710;Name=Msa0545710-mRNA-1;_AED=0.00;_eAED=0.00;_QI=111|1|1|1|1|1|2|528|291
chr4_1 exon 36270728 36271267 36270728 ID=Msa0545710-mRNA-1:exon:9188;Parent=Msa0545710-mRNA-1
chr4_1 exon 36273501 36274475 36273501 ID=Msa0545710-mRNA-1:exon:9189;Parent=Msa0545710-mRNA-1
chr4_1 five_prime_UTR 36270728 36270838 36270728 ID=Msa0545710-mRNA-1:five_prime_utr;Parent=Msa0545710-mRNA-1
chr4_1 CDS 36270839 36271267 36270839 ID=Msa0545710-mRNA-1:cds;Parent=Msa0545710-mRNA-1
chr4_1 CDS 36273501 36273947 36273501 ID=Msa0545710-mRNA-1:cds;Parent=Msa0545710-mRNA-1
chr4_1 three_prime_UTR 36273948 36274475 36273948 ID=Msa0545710-mRNA-1:three_prime_utr;Parent=Msa0545710-mRNA-1
Gene Sequence

>Msa0545710

ATGGCAATGATGGAGAGTGATGTAGAGGACAATAATTTCATTGAGTGGCTCAAACCATCTAATTCCTCTAGTTCATATCTAACTTCATCATCACCATCTTCTTCTTCCACCACTCAAAATGAAACTATACAATTCTTTCCTATTTTGAGTGCAAAGTTTTCAAAAGATGAAGAACGTCAAGTGCCACAAATTGAGGGTTTTGAGTTCAAAGAGGAGACTGTGGCTTTGCATATTGGATTACCTCATGCCACTAAAACATATCTTGATGATGAGAAGAAGTTTTTCCATGTTAAAGAAGAAGAAGAAAAGCCTTCTAAGAAAACCTTCCAGAGGTTTTGGATACCAACTCCTGCACAGATCCTTGTTGGACCTATGCAATTTTCTTGCTCCATATGCAACAAAACCTTCAATAGGTATAACAACATGCAGATGCATATGTGGGGGCATGGATCTGAATTTAGGAAAGGACCAGACTCACTAAGAGGCACACAACCAGCTGCAATGCTAAGGCTACCATGCTACTGTTGTGTTCAAGGTTGCAAGAACAACATCAATCATCCAAGAGCTAAGCCTTTGAAAGATTTTAGAACTCTCCAAACTCACTACAAAAGAAAACATGGAACAAAACCATTTATGTGTAGAAAATGTGGGAAAACATTTGCTGTGAAAGGTGATTGGAGGACTCATGAAAAGAATTGTGGCAAGTTATGGTATTGCACTTGTGGTTCAGATTTTAAACACAAAAGATCTCTCAAAGATCATATTAGGTCTTTTGGAAAAGGTCATCGTCGTCTTCCTTCGATTGATGATCAAGCTTTCGAGGAGGAGAAGGAATGTGTTACTGTCTCTGAAGAAGATGAATTTGCTCGTACATGA
Protein sequence

>Msa0545710

MAMMESDVEDNNFIEWLKPSNSSSSYLTSSSPSSSSTTQNETIQFFPILSAKFSKDEERQVPQIEGFEFKEETVALHIGLPHATKTYLDDEKKFFHVKEEEEKPSKKTFQRFWIPTPAQILVGPMQFSCSICNKTFNRYNNMQMHMWGHGSEFRKGPDSLRGTQPAAMLRLPCYCCVQGCKNNINHPRAKPLKDFRTLQTHYKRKHGTKPFMCRKCGKTFAVKGDWRTHEKNCGKLWYCTCGSDFKHKRSLKDHIRSFGKGHRRLPSIDDQAFEEEKECVTVSEEDEFART*