AlfalfaGEDB | Msa0545710

M. sativa cultivar zhongmu-4 / Msa0545710

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0008152 GO:0009058 GO:0009812 GO:0009813 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0140110 GO:1901576 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00096.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0545710	XP_024636648.1	91.864	295	20	2	1	291	1	295	4.21e-171	485

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0545710	sp\|Q9SGD1\|ZWIP3_ARATH	53.636	330	92	7	9	282	12	336	8.36e-112	329

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0545710	A0A396I4F5	91.864	295	20	2	1	291	1	295	2.01e-171	485

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TFs/TRs:

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Gene ID	Type	Classification
Msa0545710	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0545710	MtrunA17_Chr4g0026521	91.864	295	20	2	1	291	1	295	3.87e-175	485
Msa0545710	MtrunA17_Chr2g0321291	67.314	309	74	8	5	291	6	309	1.29e-132	378
Msa0545710	MtrunA17_Chr2g0328921	74.242	198	43	2	73	263	91	287	2.00e-109	319
Msa0545710	MtrunA17_Chr1g0173841	63.025	238	61	5	73	286	150	384	3.51e-106	314
Msa0545710	MtrunA17_Chr7g0258011	55.797	276	87	5	43	286	73	345	6.85e-106	311
Msa0545710	MtrunA17_Chr8g0352011	64.159	226	66	3	73	286	113	335	4.01e-105	309
Msa0545710	MtrunA17_Chr5g0422571	70.157	191	51	2	73	262	65	250	4.55e-102	300
Msa0545710	MtrunA17_Chr1g0198651	78.710	155	30	1	111	262	99	253	4.75e-96	285
Msa0545710	MtrunA17_Chr4g0053341	59.130	230	80	4	38	262	2	222	7.63e-95	279
Msa0545710	MtrunA17_Chr1g0200421	38.415	164	89	6	121	276	245	404	1.85e-26	108

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0545710	AT1G08290.1	53.636	330	92	7	9	282	12	336	8.49e-113	329
Msa0545710	AT3G57670.1	77.143	175	37	2	111	285	201	372	2.15e-102	304
Msa0545710	AT1G51220.1	52.817	284	102	5	11	262	30	313	4.41e-102	302
Msa0545710	AT3G20880.1	74.302	179	42	1	109	287	238	412	9.62e-102	303
Msa0545710	AT1G13290.1	70.312	192	47	4	73	262	55	238	5.31e-98	290
Msa0545710	AT1G34790.1	62.385	218	72	3	74	284	85	299	1.63e-94	281
Msa0545710	AT1G34370.3	37.931	174	91	7	121	285	28	193	4.54e-27	107
Msa0545710	AT1G34370.2	34.404	218	118	8	85	285	194	403	1.47e-26	108
Msa0545710	AT1G34370.1	34.404	218	118	8	85	285	194	403	1.47e-26	108
Msa0545710	AT5G22890.1	36.196	163	95	5	103	258	195	355	4.60e-24	100

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Find 72 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAATTGTGGCAAGTTA+TGG	0.431308	4_1:+36273760	Msa0545710:CDS
AAAATTGCATAGGTCCAACA+AGG	0.669488	4_1:-36271198	None:intergenic
AAAGATCATATTAGGTCTTT+TGG	0.190747	4_1:+36273825	Msa0545710:CDS
AAAGCTTGATCATCAATCGA+AGG	0.617239	4_1:-36273867	None:intergenic
AAAGGACCAGACTCACTAAG+AGG	0.575770	4_1:+36273534	Msa0545710:CDS
AACTCAAAACCCTCAATTTG+TGG	0.388963	4_1:-36271022	None:intergenic
AAGTTATGGTATTGCACTTG+TGG	0.518903	4_1:+36273774	Msa0545710:CDS
AATAAAACTAATAATTAATT+TGG	0.072784	4_1:-36270766	None:intergenic
AATGATGGAGAGTGATGTAG+AGG	0.688074	4_1:+36270844	Msa0545710:CDS
ACAAGGATCTGTGCAGGAGT+TGG	0.561642	4_1:-36271181	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCATCAAGGTAAAGAATA+TGG	+	chr4_1:36270820-36270839	Msa0545710:exon	30.0%
AGGACAATAATTTCATTGAG+TGG	+	chr4_1:36270864-36270883	Msa0545710:CDS	30.0%
TATGAACTAGAGGAATTAGA+TGG	-	chr4_1:36270896-36270915	None:intergenic	30.0%
TGAAGTTAGATATGAACTAG+AGG	-	chr4_1:36270906-36270925	None:intergenic	30.0%
GAAAACTTTGCACTCAAAAT+AGG	-	chr4_1:36270980-36270999	None:intergenic	30.0%
TATGCAACAAAACCTTCAAT+AGG	+	chr4_1:36271230-36271249	Msa0545710:CDS	30.0%
CATGTTGTTATACCTATTGA+AGG	-	chr4_1:36271245-36271264	None:intergenic	30.0%
GAAAAACTCCCCTAAAAAAA+TGG	+	chr4_1:36271369-36271388	Msa0545710:intron	30.0%
TAAAAACTATAAGGTCTAGC+AGG	+	chr4_1:36271548-36271567	Msa0545710:intron	30.0%
ATAGATTAGCACCTACATTT+AGG	+	chr4_1:36272194-36272213	Msa0545710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	36270728	36274475	36270728	ID=Msa0545710;Name=Msa0545710
chr4_1	mRNA	36270728	36274475	36270728	ID=Msa0545710-mRNA-1;Parent=Msa0545710;Name=Msa0545710-mRNA-1;_AED=0.00;_eAED=0.00;_QI=111\|1\|1\|1\|1\|1\|2\|528\|291
chr4_1	exon	36270728	36271267	36270728	ID=Msa0545710-mRNA-1:exon:9188;Parent=Msa0545710-mRNA-1
chr4_1	exon	36273501	36274475	36273501	ID=Msa0545710-mRNA-1:exon:9189;Parent=Msa0545710-mRNA-1
chr4_1	five_prime_UTR	36270728	36270838	36270728	ID=Msa0545710-mRNA-1:five_prime_utr;Parent=Msa0545710-mRNA-1
chr4_1	CDS	36270839	36271267	36270839	ID=Msa0545710-mRNA-1:cds;Parent=Msa0545710-mRNA-1
chr4_1	CDS	36273501	36273947	36273501	ID=Msa0545710-mRNA-1:cds;Parent=Msa0545710-mRNA-1
chr4_1	three_prime_UTR	36273948	36274475	36273948	ID=Msa0545710-mRNA-1:three_prime_utr;Parent=Msa0545710-mRNA-1

Gene Sequence

>Msa0545710

ATGGCAATGATGGAGAGTGATGTAGAGGACAATAATTTCATTGAGTGGCTCAAACCATCTAATTCCTCTAGTTCATATCTAACTTCATCATCACCATCTTCTTCTTCCACCACTCAAAATGAAACTATACAATTCTTTCCTATTTTGAGTGCAAAGTTTTCAAAAGATGAAGAACGTCAAGTGCCACAAATTGAGGGTTTTGAGTTCAAAGAGGAGACTGTGGCTTTGCATATTGGATTACCTCATGCCACTAAAACATATCTTGATGATGAGAAGAAGTTTTTCCATGTTAAAGAAGAAGAAGAAAAGCCTTCTAAGAAAACCTTCCAGAGGTTTTGGATACCAACTCCTGCACAGATCCTTGTTGGACCTATGCAATTTTCTTGCTCCATATGCAACAAAACCTTCAATAGGTATAACAACATGCAGATGCATATGTGGGGGCATGGATCTGAATTTAGGAAAGGACCAGACTCACTAAGAGGCACACAACCAGCTGCAATGCTAAGGCTACCATGCTACTGTTGTGTTCAAGGTTGCAAGAACAACATCAATCATCCAAGAGCTAAGCCTTTGAAAGATTTTAGAACTCTCCAAACTCACTACAAAAGAAAACATGGAACAAAACCATTTATGTGTAGAAAATGTGGGAAAACATTTGCTGTGAAAGGTGATTGGAGGACTCATGAAAAGAATTGTGGCAAGTTATGGTATTGCACTTGTGGTTCAGATTTTAAACACAAAAGATCTCTCAAAGATCATATTAGGTCTTTTGGAAAAGGTCATCGTCGTCTTCCTTCGATTGATGATCAAGCTTTCGAGGAGGAGAAGGAATGTGTTACTGTCTCTGAAGAAGATGAATTTGCTCGTACATGA

Protein sequence

>Msa0545710

MAMMESDVEDNNFIEWLKPSNSSSSYLTSSSPSSSSTTQNETIQFFPILSAKFSKDEERQVPQIEGFEFKEETVALHIGLPHATKTYLDDEKKFFHVKEEEEKPSKKTFQRFWIPTPAQILVGPMQFSCSICNKTFNRYNNMQMHMWGHGSEFRKGPDSLRGTQPAAMLRLPCYCCVQGCKNNINHPRAKPLKDFRTLQTHYKRKHGTKPFMCRKCGKTFAVKGDWRTHEKNCGKLWYCTCGSDFKHKRSLKDHIRSFGKGHRRLPSIDDQAFEEEKECVTVSEEDEFART*