AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0549060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549060 MtrunA17_Chr4g0025531 55.378 251 91 5 1 248 1 233 1.09e-82 247
Msa0549060 MtrunA17_Chr3g0135891 56.081 148 58 5 94 238 3 146 9.22e-42 139
Msa0549060 MtrunA17_Chr2g0292791 37.725 167 87 4 1 150 1 167 1.48e-22 92.4
Msa0549060 MtrunA17_Chr3g0099421 37.126 167 88 5 1 150 26 192 1.67e-22 92.8
Msa0549060 MtrunA17_Chr1g0149391 37.931 174 86 8 1 156 1 170 2.37e-22 92.0
Msa0549060 MtrunA17_Chr1g0206701 37.267 161 84 4 1 144 1 161 2.68e-22 92.0
Msa0549060 MtrunA17_Chr8g0355091 37.086 151 80 3 1 136 1 151 5.54e-22 90.9
Msa0549060 MtrunA17_Chr1g0206991 37.267 161 84 5 1 144 1 161 1.53e-21 88.6
Msa0549060 MtrunA17_Chr1g0208561 33.133 166 98 3 1 156 1 163 2.09e-21 89.4
Msa0549060 MtrunA17_Chr1g0188671 32.530 166 97 4 1 155 39 200 2.84e-21 90.1
Msa0549060 MtrunA17_Chr3g0089141 33.133 166 98 3 1 156 1 163 4.36e-21 88.6
Msa0549060 MtrunA17_Chr3g0119901 34.337 166 94 4 1 155 1 162 6.13e-21 87.4
Msa0549060 MtrunA17_Chr5g0422421 30.698 215 115 8 1 201 1 195 1.18e-20 87.4
Msa0549060 MtrunA17_Chr3g0085241 31.222 221 121 7 4 202 36 247 3.02e-20 86.7
Msa0549060 MtrunA17_Chr1g0206721 36.424 151 81 3 1 136 1 151 5.33e-20 85.9
Msa0549060 MtrunA17_Chr2g0317481 31.250 160 99 3 1 151 1 158 5.61e-20 85.5
Msa0549060 MtrunA17_Chr2g0317451 31.250 160 99 3 1 151 1 158 5.72e-20 85.5
Msa0549060 MtrunA17_Chr1g0187361 29.545 176 108 4 1 163 1 173 1.28e-19 84.7
Msa0549060 MtrunA17_Chr8g0379021 31.579 171 84 4 4 156 7 162 3.31e-19 82.0
Msa0549060 MtrunA17_Chr5g0419601 34.307 137 80 3 1 127 1 137 3.39e-19 83.6
Msa0549060 MtrunA17_Chr3g0099411 52.778 72 34 0 1 72 1 72 4.03e-19 82.8
Msa0549060 MtrunA17_Chr1g0208581 49.351 77 39 0 1 77 1 77 4.30e-19 83.2
Msa0549060 MtrunA17_Chr5g0422941 27.966 236 132 8 1 202 1 232 4.94e-18 80.5
Msa0549060 MtrunA17_Chr2g0333981 30.818 159 101 3 4 156 7 162 9.62e-18 78.2
Msa0549060 MtrunA17_Chr2g0325971 32.298 161 92 4 1 144 1 161 1.33e-17 79.0
Msa0549060 MtrunA17_Chr3g0111841 53.448 58 27 0 15 72 2 59 3.05e-16 71.2
Msa0549060 MtrunA17_Chr3g0089151 39.252 107 62 2 1 104 1 107 3.79e-16 72.8
Msa0549060 MtrunA17_Chr2g0300421 44.156 77 43 0 1 77 1 77 4.10e-16 73.9
Msa0549060 MtrunA17_Chr4g0052081 50.725 69 34 0 9 77 2 70 4.99e-16 73.6
Msa0549060 MtrunA17_Chr1g0206691 29.082 196 113 6 18 203 1 180 2.72e-15 72.8
Msa0549060 MtrunA17_Chr2g0300441 30.723 166 98 4 1 149 1 166 6.81e-15 71.6
Msa0549060 MtrunA17_Chr8g0356071 35.345 116 56 3 9 115 2 107 1.34e-14 69.3
Msa0549060 MtrunA17_Chr4g0052061 33.333 144 84 4 9 151 2 134 1.97e-14 68.9
Msa0549060 MtrunA17_Chr1g0169761 36.522 115 61 2 9 115 2 112 2.91e-14 68.9
Msa0549060 MtrunA17_Chr8g0376821 36.607 112 67 2 9 116 2 113 4.31e-14 68.6
Msa0549060 MtrunA17_Chr8g0345001 32.515 163 94 4 8 159 17 174 5.51e-14 68.2
Msa0549060 MtrunA17_Chr8g0357771 32.143 140 71 4 9 126 2 139 1.50e-13 67.0
Msa0549060 MtrunA17_Chr1g0175611 40.000 70 42 0 9 78 2 71 1.76e-12 63.9
Msa0549060 MtrunA17_Chr4g0074101 38.889 72 44 0 9 80 2 73 1.96e-12 64.3
Msa0549060 MtrunA17_Chr1g0180591 35.714 98 62 1 9 105 2 99 5.07e-12 62.8
Msa0549060 MtrunA17_Chr1g0159661 50.000 64 32 0 9 72 2 65 5.70e-12 62.8
Msa0549060 MtrunA17_Chr4g0057151 49.254 67 31 1 10 76 3 66 2.23e-11 61.6
Msa0549060 MtrunA17_Chr1g0187351 38.462 65 40 0 18 82 1 65 2.36e-11 58.2
Msa0549060 MtrunA17_Chr4g0018951 29.921 127 83 4 26 148 29 153 2.66e-11 60.5
Msa0549060 MtrunA17_Chr1g0175601 40.000 65 39 0 9 73 2 66 3.44e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549060 AT4G36590.1 35.329 167 91 3 5 156 3 167 5.63e-23 94.4
Msa0549060 AT1G29962.1 35.227 176 96 4 1 161 1 173 3.54e-22 90.5
Msa0549060 AT2G24840.1 32.474 194 109 6 7 187 61 245 1.03e-20 88.6
Msa0549060 AT1G28460.1 35.260 173 97 4 1 161 1 170 2.91e-20 85.5
Msa0549060 AT1G01530.1 38.136 118 67 2 5 117 3 119 1.34e-18 82.4
Msa0549060 AT5G60440.1 39.007 141 68 4 5 144 3 126 3.83e-18 82.0
Msa0549060 AT1G28450.1 32.759 174 99 4 1 159 1 171 3.28e-17 77.4
Msa0549060 AT2G34440.1 31.609 174 102 7 9 174 2 166 6.50e-17 76.3
Msa0549060 AT3G04100.1 41.573 89 49 1 2 90 7 92 9.96e-16 73.9
Msa0549060 AT1G65360.1 33.962 106 66 1 5 106 3 108 1.43e-15 73.9
Msa0549060 AT3G66656.1 47.692 65 34 0 9 73 2 66 5.91e-14 68.6
Msa0549060 AT1G47760.1 39.759 83 47 1 9 91 2 81 7.34e-13 65.5
Msa0549060 AT4G11880.2 49.254 67 32 1 10 76 3 67 5.99e-11 60.1
Msa0549060 AT4G11880.3 49.254 67 32 1 10 76 3 67 5.99e-11 60.1
Msa0549060 AT4G11880.4 49.254 67 32 1 10 76 3 67 8.35e-11 60.5
Msa0549060 AT4G11880.1 49.254 67 32 1 10 76 3 67 8.35e-11 60.5
Msa0549060 AT5G10140.2 48.214 56 29 0 9 64 2 57 9.44e-11 59.3
Msa0549060 AT1G72350.1 47.541 61 32 0 9 69 43 103 9.55e-11 60.5

Find 56 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCATATCATTCATCTTT+AGG 0.191781 4_1:+43806486 None:intergenic
ATCAATCCTCCTTATGTCTT+TGG 0.213662 4_1:-43806902 Msa0549060:CDS
TCAATCCTCCTTATGTCTTT+GGG 0.241289 4_1:-43806901 Msa0549060:CDS
AACAACAACGGTTATGAAAT+TGG 0.254239 4_1:-43806875 Msa0549060:CDS
ATCATATATACCTTGGAATA+AGG 0.308845 4_1:-43806443 Msa0549060:three_prime_UTR
ACGGGAAAGTTTGAAGGCTT+TGG 0.328236 4_1:-43807228 Msa0549060:CDS
ATAAGCGGACATCACATTTC+AGG 0.330823 4_1:-43806416 Msa0549060:three_prime_UTR
GAAGGTTCTCCATAACAATA+TGG 0.353627 4_1:+43807386 None:intergenic
CTTTGGAAAGTTATGGCTAT+TGG 0.358280 4_1:-43807211 Msa0549060:CDS
AAAATTGGACACATCTTTAG+TGG 0.370003 4_1:-43807141 Msa0549060:CDS
GATGTTATTGCTTCTCTTGA+AGG 0.392101 4_1:-43806582 Msa0549060:CDS
TCTGAATTATCAACACTTTG+TGG 0.392582 4_1:-43807449 Msa0549060:CDS
TTGAAGGCTTTGGAAAGTTA+TGG 0.393387 4_1:-43807218 Msa0549060:CDS
CTTGCTATGGATTGAGAAGA+AGG 0.408194 4_1:+43807368 None:intergenic
GCATGTTGCGGAAGCACAAC+GGG 0.408411 4_1:-43807246 Msa0549060:CDS
GCAGGAAAACCATATTGTTA+TGG 0.420932 4_1:-43807395 Msa0549060:CDS
TCACTTGCACGGTCAAGGTT+TGG 0.423194 4_1:+43806540 None:intergenic
CTGAATTATCAACACTTTGT+GGG 0.424852 4_1:-43807448 Msa0549060:CDS
CTACCTCTTGATGTCCTCTT+CGG 0.429458 4_1:+43807560 None:intergenic
CTTCAAAATTGTTCTTGCTA+TGG 0.431100 4_1:+43807355 None:intergenic
CATCAAAATGAGAAAATAGT+TGG 0.433292 4_1:+43806381 None:intergenic
GGTATATATGATCATACATA+TGG 0.438624 4_1:+43806454 None:intergenic
TTTAATTCTTCTTCTACTGA+AGG 0.442448 4_1:+43807323 None:intergenic
CATTTCCACTTTCACTTGCA+CGG 0.448306 4_1:+43806529 None:intergenic
ATTCTTATTATTTCTCCAGC+AGG 0.453510 4_1:-43807413 Msa0549060:CDS
TGAGAAAATAGTTGGAATCA+TGG 0.457232 4_1:+43806389 None:intergenic
CACTTTCACTTGCACGGTCA+AGG 0.460125 4_1:+43806535 None:intergenic
AATTTCATAACCGTTGTTGT+TGG 0.462889 4_1:+43806877 None:intergenic
CTTGACCGTGCAAGTGAAAG+TGG 0.475104 4_1:-43806534 Msa0549060:CDS
TTGTTGGCCCCAAAGACATA+AGG 0.478695 4_1:+43806893 None:intergenic
TTAATTCTTCTTCTACTGAA+GGG 0.481788 4_1:+43807324 None:intergenic
AGACATAAGGAGGATTGATC+TGG 0.482662 4_1:+43806906 None:intergenic
CGTGCAAGTGAAAGTGGAAA+TGG 0.489776 4_1:-43806528 Msa0549060:CDS
AGCACAACGGGAAAGTTTGA+AGG 0.506480 4_1:-43807234 Msa0549060:CDS
ATTTATGATCCAAAAGGAAA+AGG 0.506756 4_1:-43806609 Msa0549060:CDS
TCCCAGATTTATGATCCAAA+AGG 0.515467 4_1:-43806615 Msa0549060:CDS
TAAGCGGACATCACATTTCA+GGG 0.527919 4_1:-43806415 Msa0549060:three_prime_UTR
TCTTGCATAGACGAACTGAA+TGG 0.536180 4_1:-43806932 Msa0549060:CDS
ATAAGTAGTGTCTTAAGCAA+TGG 0.548667 4_1:-43807101 Msa0549060:CDS
GCTTATATCACCTTATTCCA+AGG 0.555747 4_1:+43806433 None:intergenic
ATGTATGATCATATATACCT+TGG 0.560368 4_1:-43806450 Msa0549060:three_prime_UTR
TGAATTATCAACACTTTGTG+GGG 0.569118 4_1:-43807447 Msa0549060:CDS
GTCTTTGGGGCCAACAACAA+CGG 0.574083 4_1:-43806887 Msa0549060:CDS
CAATCCTCCTTATGTCTTTG+GGG 0.592798 4_1:-43806900 Msa0549060:CDS
GGAGGAGAGATTAATCCTCA+TGG 0.604055 4_1:-43807080 Msa0549060:intron
AGTAGTGTCTTAAGCAATGG+AGG 0.611596 4_1:-43807098 Msa0549060:CDS
TTGGCCCCAAAGACATAAGG+AGG 0.611952 4_1:+43806896 None:intergenic
ACAAAGTGTTGATAATTCAG+AGG 0.616841 4_1:+43807451 None:intergenic
TGATCCAAAAGGAAAAGGCA+AGG 0.624272 4_1:-43806604 Msa0549060:CDS
AGCATGTTGCGGAAGCACAA+CGG 0.628936 4_1:-43807247 Msa0549060:CDS
TTTGGATGAAAAGCATGTTG+CGG 0.629913 4_1:-43807258 Msa0549060:CDS
TGGTTAAGATGGCTCCGAAG+AGG 0.660173 4_1:-43807574 None:intergenic
TTGGAATAAGGTGATATAAG+CGG 0.660825 4_1:-43806431 Msa0549060:three_prime_UTR
GCTCCGAAGAGGACATCAAG+AGG 0.673948 4_1:-43807563 Msa0549060:CDS
GAAGGTAGAAATAAAACACA+AGG 0.705496 4_1:-43806564 Msa0549060:CDS
GCGGACATCACATTTCAGGG+TGG 0.773228 4_1:-43806412 Msa0549060:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATTTATGAAAAAG+TGG - chr4_1:43806833-43806852 Msa0549060:intron 15.0%
!! CTAATTTATTTATGAAAAAG+TGG - chr4_1:43806833-43806852 Msa0549060:intron 15.0%
!! TTATGACCAGTTAAAAAAAT+TGG - chr4_1:43806654-43806673 Msa0549060:intron 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG + chr4_1:43807106-43807125 None:intergenic 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG + chr4_1:43807107-43807126 None:intergenic 20.0%
!! GAAATTATACAATATGTGTT+AGG + chr4_1:43807155-43807174 None:intergenic 20.0%
!!! TTTTCTATATTCTACTTTTG+TGG - chr4_1:43807488-43807507 Msa0549060:CDS 20.0%
!! TTATGACCAGTTAAAAAAAT+TGG - chr4_1:43806654-43806673 Msa0549060:intron 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG + chr4_1:43807106-43807125 None:intergenic 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG + chr4_1:43807107-43807126 None:intergenic 20.0%
!! GAAATTATACAATATGTGTT+AGG + chr4_1:43807155-43807174 None:intergenic 20.0%
!!! TTTTCTATATTCTACTTTTG+TGG - chr4_1:43807488-43807507 Msa0549060:CDS 20.0%
! TTAATTCTTCTTCTACTGAA+GGG + chr4_1:43806489-43806508 None:intergenic 25.0%
! TTTAATTCTTCTTCTACTGA+AGG + chr4_1:43806490-43806509 None:intergenic 25.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG + chr4_1:43806663-43806682 None:intergenic 25.0%
! TTACTCGTGTTATATGAATT+TGG + chr4_1:43806981-43807000 None:intergenic 25.0%
! AGTATAGATATTTGATGTGT+TGG + chr4_1:43807021-43807040 None:intergenic 25.0%
! GCATTAAAAATGGATAAAGT+TGG + chr4_1:43807045-43807064 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG + chr4_1:43807055-43807074 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG - chr4_1:43807201-43807220 Msa0549060:CDS 25.0%
! GGTATATATGATCATACATA+TGG + chr4_1:43807359-43807378 None:intergenic 25.0%
! ATGTATGATCATATATACCT+TGG - chr4_1:43807360-43807379 Msa0549060:CDS 25.0%
! ATCATATATACCTTGGAATA+AGG - chr4_1:43807367-43807386 Msa0549060:CDS 25.0%
! CATCAAAATGAGAAAATAGT+TGG + chr4_1:43807432-43807451 None:intergenic 25.0%
!!! TTTGTGGATTTTTATTTGAG+TGG - chr4_1:43807504-43807523 Msa0549060:CDS 25.0%
!!! TCAGTTTTTTTTTTGTGCTT+TGG - chr4_1:43807548-43807567 Msa0549060:CDS 25.0%
!!! TTTTTTTGTGCTTTGGATTT+TGG - chr4_1:43807555-43807574 Msa0549060:CDS 25.0%
! TTAATTCTTCTTCTACTGAA+GGG + chr4_1:43806489-43806508 None:intergenic 25.0%
! TTTAATTCTTCTTCTACTGA+AGG + chr4_1:43806490-43806509 None:intergenic 25.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG + chr4_1:43806663-43806682 None:intergenic 25.0%
! TTACTCGTGTTATATGAATT+TGG + chr4_1:43806981-43807000 None:intergenic 25.0%
! AGTATAGATATTTGATGTGT+TGG + chr4_1:43807021-43807040 None:intergenic 25.0%
! GCATTAAAAATGGATAAAGT+TGG + chr4_1:43807045-43807064 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG + chr4_1:43807055-43807074 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG - chr4_1:43807201-43807220 Msa0549060:CDS 25.0%
! GGTATATATGATCATACATA+TGG + chr4_1:43807359-43807378 None:intergenic 25.0%
! ATGTATGATCATATATACCT+TGG - chr4_1:43807360-43807379 Msa0549060:CDS 25.0%
! ATCATATATACCTTGGAATA+AGG - chr4_1:43807367-43807386 Msa0549060:CDS 25.0%
! CATCAAAATGAGAAAATAGT+TGG + chr4_1:43807432-43807451 None:intergenic 25.0%
!!! TTTGTGGATTTTTATTTGAG+TGG - chr4_1:43807504-43807523 Msa0549060:CDS 25.0%
!!! TCAGTTTTTTTTTTGTGCTT+TGG - chr4_1:43807548-43807567 Msa0549060:CDS 25.0%
!!! TTTTTTTGTGCTTTGGATTT+TGG - chr4_1:43807555-43807574 Msa0549060:CDS 25.0%
!! ACAAAGTGTTGATAATTCAG+AGG + chr4_1:43806362-43806381 None:intergenic 30.0%
TCTGAATTATCAACACTTTG+TGG - chr4_1:43806361-43806380 Msa0549060:three_prime_UTR 30.0%
CTGAATTATCAACACTTTGT+GGG - chr4_1:43806362-43806381 Msa0549060:three_prime_UTR 30.0%
TGAATTATCAACACTTTGTG+GGG - chr4_1:43806363-43806382 Msa0549060:three_prime_UTR 30.0%
! ATTCTTATTATTTCTCCAGC+AGG - chr4_1:43806397-43806416 Msa0549060:three_prime_UTR 30.0%
CTTCAAAATTGTTCTTGCTA+TGG + chr4_1:43806458-43806477 None:intergenic 30.0%
! CACAAAAAATGATGAGCTTT+TGG - chr4_1:43806534-43806553 Msa0549060:CDS 30.0%
AAAATTGGACACATCTTTAG+TGG - chr4_1:43806669-43806688 Msa0549060:intron 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG - chr4_1:43806709-43806728 Msa0549060:intron 30.0%
TGTACATCTTCTAATCAACT+TGG - chr4_1:43806757-43806776 Msa0549060:intron 30.0%
AATTTCATAACCGTTGTTGT+TGG + chr4_1:43806936-43806955 None:intergenic 30.0%
AACAACAACGGTTATGAAAT+TGG - chr4_1:43806935-43806954 Msa0549060:CDS 30.0%
! TCCTTTTGGATCATAAATCT+GGG + chr4_1:43807199-43807218 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATC+TGG + chr4_1:43807200-43807219 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG - chr4_1:43807246-43807265 Msa0549060:CDS 30.0%
CTAGCATATCATTCATCTTT+AGG + chr4_1:43807327-43807346 None:intergenic 30.0%
TTGGAATAAGGTGATATAAG+CGG - chr4_1:43807379-43807398 Msa0549060:CDS 30.0%
TGAGAAAATAGTTGGAATCA+TGG + chr4_1:43807424-43807443 None:intergenic 30.0%
!! TGGATTTTTATTTGAGTGGT+TGG - chr4_1:43807508-43807527 Msa0549060:CDS 30.0%
!!! CTTTGGATTTTGGTTGTATT+GGG - chr4_1:43807565-43807584 Msa0549060:CDS 30.0%
!! ACAAAGTGTTGATAATTCAG+AGG + chr4_1:43806362-43806381 None:intergenic 30.0%
TCTGAATTATCAACACTTTG+TGG - chr4_1:43806361-43806380 Msa0549060:three_prime_UTR 30.0%
CTGAATTATCAACACTTTGT+GGG - chr4_1:43806362-43806381 Msa0549060:three_prime_UTR 30.0%
TGAATTATCAACACTTTGTG+GGG - chr4_1:43806363-43806382 Msa0549060:three_prime_UTR 30.0%
! ATTCTTATTATTTCTCCAGC+AGG - chr4_1:43806397-43806416 Msa0549060:three_prime_UTR 30.0%
CTTCAAAATTGTTCTTGCTA+TGG + chr4_1:43806458-43806477 None:intergenic 30.0%
! CACAAAAAATGATGAGCTTT+TGG - chr4_1:43806534-43806553 Msa0549060:CDS 30.0%
AAAATTGGACACATCTTTAG+TGG - chr4_1:43806669-43806688 Msa0549060:intron 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG - chr4_1:43806709-43806728 Msa0549060:intron 30.0%
TGTACATCTTCTAATCAACT+TGG - chr4_1:43806757-43806776 Msa0549060:intron 30.0%
AATTTCATAACCGTTGTTGT+TGG + chr4_1:43806936-43806955 None:intergenic 30.0%
AACAACAACGGTTATGAAAT+TGG - chr4_1:43806935-43806954 Msa0549060:CDS 30.0%
! TCCTTTTGGATCATAAATCT+GGG + chr4_1:43807199-43807218 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATC+TGG + chr4_1:43807200-43807219 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG - chr4_1:43807246-43807265 Msa0549060:CDS 30.0%
CTAGCATATCATTCATCTTT+AGG + chr4_1:43807327-43807346 None:intergenic 30.0%
TTGGAATAAGGTGATATAAG+CGG - chr4_1:43807379-43807398 Msa0549060:CDS 30.0%
TGAGAAAATAGTTGGAATCA+TGG + chr4_1:43807424-43807443 None:intergenic 30.0%
!! TGGATTTTTATTTGAGTGGT+TGG - chr4_1:43807508-43807527 Msa0549060:CDS 30.0%
!!! CTTTGGATTTTGGTTGTATT+GGG - chr4_1:43807565-43807584 Msa0549060:CDS 30.0%
! GCATTTTCAAAGAGAAAGTC+TGG - chr4_1:43806322-43806341 Msa0549060:three_prime_UTR 35.0%
! TAACAATATGGTTTTCCTGC+TGG + chr4_1:43806415-43806434 None:intergenic 35.0%
GCAGGAAAACCATATTGTTA+TGG - chr4_1:43806415-43806434 Msa0549060:three_prime_UTR 35.0%
GAAGGTTCTCCATAACAATA+TGG + chr4_1:43806427-43806446 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG - chr4_1:43806552-43806571 Msa0549060:CDS 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG - chr4_1:43806592-43806611 Msa0549060:CDS 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG - chr4_1:43806599-43806618 Msa0549060:CDS 35.0%
ATCAATCCTCCTTATGTCTT+TGG - chr4_1:43806908-43806927 Msa0549060:CDS 35.0%
TCAATCCTCCTTATGTCTTT+GGG - chr4_1:43806909-43806928 Msa0549060:CDS 35.0%
TATGTGTTAGGTTTATCGCA+TGG + chr4_1:43807143-43807162 None:intergenic 35.0%
TTTCTTGTCATTGAAGCTCA+TGG - chr4_1:43807171-43807190 Msa0549060:CDS 35.0%
TCCCAGATTTATGATCCAAA+AGG - chr4_1:43807195-43807214 Msa0549060:CDS 35.0%
GATGTTATTGCTTCTCTTGA+AGG - chr4_1:43807228-43807247 Msa0549060:CDS 35.0%
GCTTATATCACCTTATTCCA+AGG + chr4_1:43807380-43807399 None:intergenic 35.0%
!!! GCTTTGGATTTTGGTTGTAT+TGG - chr4_1:43807564-43807583 Msa0549060:CDS 35.0%
! GCATTTTCAAAGAGAAAGTC+TGG - chr4_1:43806322-43806341 Msa0549060:three_prime_UTR 35.0%
! TAACAATATGGTTTTCCTGC+TGG + chr4_1:43806415-43806434 None:intergenic 35.0%
GCAGGAAAACCATATTGTTA+TGG - chr4_1:43806415-43806434 Msa0549060:three_prime_UTR 35.0%
GAAGGTTCTCCATAACAATA+TGG + chr4_1:43806427-43806446 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG - chr4_1:43806552-43806571 Msa0549060:CDS 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG - chr4_1:43806592-43806611 Msa0549060:CDS 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG - chr4_1:43806599-43806618 Msa0549060:CDS 35.0%
ATCAATCCTCCTTATGTCTT+TGG - chr4_1:43806908-43806927 Msa0549060:CDS 35.0%
TCAATCCTCCTTATGTCTTT+GGG - chr4_1:43806909-43806928 Msa0549060:CDS 35.0%
TATGTGTTAGGTTTATCGCA+TGG + chr4_1:43807143-43807162 None:intergenic 35.0%
TTTCTTGTCATTGAAGCTCA+TGG - chr4_1:43807171-43807190 Msa0549060:CDS 35.0%
TCCCAGATTTATGATCCAAA+AGG - chr4_1:43807195-43807214 Msa0549060:CDS 35.0%
GATGTTATTGCTTCTCTTGA+AGG - chr4_1:43807228-43807247 Msa0549060:CDS 35.0%
GCTTATATCACCTTATTCCA+AGG + chr4_1:43807380-43807399 None:intergenic 35.0%
!!! GCTTTGGATTTTGGTTGTAT+TGG - chr4_1:43807564-43807583 Msa0549060:CDS 35.0%
! CTTGCTATGGATTGAGAAGA+AGG + chr4_1:43806445-43806464 None:intergenic 40.0%
AGTAGTGTCTTAAGCAATGG+AGG - chr4_1:43806712-43806731 Msa0549060:intron 40.0%
TCTTGCATAGACGAACTGAA+TGG - chr4_1:43806878-43806897 Msa0549060:CDS 40.0%
AGACATAAGGAGGATTGATC+TGG + chr4_1:43806907-43806926 None:intergenic 40.0%
CAATCCTCCTTATGTCTTTG+GGG - chr4_1:43806910-43806929 Msa0549060:CDS 40.0%
TGATCCAAAAGGAAAAGGCA+AGG - chr4_1:43807206-43807225 Msa0549060:CDS 40.0%
! ACATCCTTGCCTTTTCCTTT+TGG + chr4_1:43807213-43807232 None:intergenic 40.0%
CATTTCCACTTTCACTTGCA+CGG + chr4_1:43807284-43807303 None:intergenic 40.0%
ATAAGCGGACATCACATTTC+AGG - chr4_1:43807394-43807413 Msa0549060:CDS 40.0%
TAAGCGGACATCACATTTCA+GGG - chr4_1:43807395-43807414 Msa0549060:CDS 40.0%
! CTTGCTATGGATTGAGAAGA+AGG + chr4_1:43806445-43806464 None:intergenic 40.0%
AGTAGTGTCTTAAGCAATGG+AGG - chr4_1:43806712-43806731 Msa0549060:intron 40.0%
TCTTGCATAGACGAACTGAA+TGG - chr4_1:43806878-43806897 Msa0549060:CDS 40.0%
AGACATAAGGAGGATTGATC+TGG + chr4_1:43806907-43806926 None:intergenic 40.0%
CAATCCTCCTTATGTCTTTG+GGG - chr4_1:43806910-43806929 Msa0549060:CDS 40.0%
TGATCCAAAAGGAAAAGGCA+AGG - chr4_1:43807206-43807225 Msa0549060:CDS 40.0%
! ACATCCTTGCCTTTTCCTTT+TGG + chr4_1:43807213-43807232 None:intergenic 40.0%
CATTTCCACTTTCACTTGCA+CGG + chr4_1:43807284-43807303 None:intergenic 40.0%
ATAAGCGGACATCACATTTC+AGG - chr4_1:43807394-43807413 Msa0549060:CDS 40.0%
TAAGCGGACATCACATTTCA+GGG - chr4_1:43807395-43807414 Msa0549060:CDS 40.0%
CTACCTCTTGATGTCCTCTT+CGG + chr4_1:43806253-43806272 None:intergenic 45.0%
AGCACAACGGGAAAGTTTGA+AGG - chr4_1:43806576-43806595 Msa0549060:CDS 45.0%
ACGGGAAAGTTTGAAGGCTT+TGG - chr4_1:43806582-43806601 Msa0549060:CDS 45.0%
GGAGGAGAGATTAATCCTCA+TGG - chr4_1:43806730-43806749 Msa0549060:intron 45.0%
GAAGATGTACACGTACCATG+AGG + chr4_1:43806748-43806767 None:intergenic 45.0%
TTGTTGGCCCCAAAGACATA+AGG + chr4_1:43806920-43806939 None:intergenic 45.0%
CGTGCAAGTGAAAGTGGAAA+TGG - chr4_1:43807282-43807301 Msa0549060:CDS 45.0%
CTACCTCTTGATGTCCTCTT+CGG + chr4_1:43806253-43806272 None:intergenic 45.0%
AGCACAACGGGAAAGTTTGA+AGG - chr4_1:43806576-43806595 Msa0549060:CDS 45.0%
ACGGGAAAGTTTGAAGGCTT+TGG - chr4_1:43806582-43806601 Msa0549060:CDS 45.0%
GGAGGAGAGATTAATCCTCA+TGG - chr4_1:43806730-43806749 Msa0549060:intron 45.0%
GAAGATGTACACGTACCATG+AGG + chr4_1:43806748-43806767 None:intergenic 45.0%
TTGTTGGCCCCAAAGACATA+AGG + chr4_1:43806920-43806939 None:intergenic 45.0%
CGTGCAAGTGAAAGTGGAAA+TGG - chr4_1:43807282-43807301 Msa0549060:CDS 45.0%
! AGCATGTTGCGGAAGCACAA+CGG - chr4_1:43806563-43806582 Msa0549060:CDS 50.0%
TTGGCCCCAAAGACATAAGG+AGG + chr4_1:43806917-43806936 None:intergenic 50.0%
!! GTCTTTGGGGCCAACAACAA+CGG - chr4_1:43806923-43806942 Msa0549060:CDS 50.0%
TCACTTGCACGGTCAAGGTT+TGG + chr4_1:43807273-43807292 None:intergenic 50.0%
CACTTTCACTTGCACGGTCA+AGG + chr4_1:43807278-43807297 None:intergenic 50.0%
CTTGACCGTGCAAGTGAAAG+TGG - chr4_1:43807276-43807295 Msa0549060:CDS 50.0%
! AGCATGTTGCGGAAGCACAA+CGG - chr4_1:43806563-43806582 Msa0549060:CDS 50.0%
TTGGCCCCAAAGACATAAGG+AGG + chr4_1:43806917-43806936 None:intergenic 50.0%
!! GTCTTTGGGGCCAACAACAA+CGG - chr4_1:43806923-43806942 Msa0549060:CDS 50.0%
TCACTTGCACGGTCAAGGTT+TGG + chr4_1:43807273-43807292 None:intergenic 50.0%
CACTTTCACTTGCACGGTCA+AGG + chr4_1:43807278-43807297 None:intergenic 50.0%
CTTGACCGTGCAAGTGAAAG+TGG - chr4_1:43807276-43807295 Msa0549060:CDS 50.0%
GCTCCGAAGAGGACATCAAG+AGG - chr4_1:43806247-43806266 Msa0549060:three_prime_UTR 55.0%
! GCATGTTGCGGAAGCACAAC+GGG - chr4_1:43806564-43806583 Msa0549060:CDS 55.0%
GCGGACATCACATTTCAGGG+TGG - chr4_1:43807398-43807417 Msa0549060:CDS 55.0%
GCTCCGAAGAGGACATCAAG+AGG - chr4_1:43806247-43806266 Msa0549060:three_prime_UTR 55.0%
! GCATGTTGCGGAAGCACAAC+GGG - chr4_1:43806564-43806583 Msa0549060:CDS 55.0%
GCGGACATCACATTTCAGGG+TGG - chr4_1:43807398-43807417 Msa0549060:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 43806244 43807588 43806244 ID=Msa0549060;Name=Msa0549060
chr4_1 mRNA 43806244 43807588 43806244 ID=Msa0549060-mRNA-1;Parent=Msa0549060;Name=Msa0549060-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0.5|0.33|1|1|1|3|250|249
chr4_1 exon 43806244 43806645 43806244 ID=Msa0549060-mRNA-1:exon:11319;Parent=Msa0549060-mRNA-1
chr4_1 exon 43806870 43806959 43806870 ID=Msa0549060-mRNA-1:exon:11318;Parent=Msa0549060-mRNA-1
chr4_1 exon 43807081 43807588 43807081 ID=Msa0549060-mRNA-1:exon:11317;Parent=Msa0549060-mRNA-1
chr4_1 CDS 43807081 43807588 43807081 ID=Msa0549060-mRNA-1:cds;Parent=Msa0549060-mRNA-1
chr4_1 CDS 43806870 43806959 43806870 ID=Msa0549060-mRNA-1:cds;Parent=Msa0549060-mRNA-1
chr4_1 CDS 43806494 43806645 43806494 ID=Msa0549060-mRNA-1:cds;Parent=Msa0549060-mRNA-1
chr4_1 three_prime_UTR 43806244 43806493 43806244 ID=Msa0549060-mRNA-1:three_prime_utr;Parent=Msa0549060-mRNA-1
Gene Sequence

>Msa0549060

ATGGCTCCGAAGAGGACATCAAGAGGTAGACAAAAAAGAGAAATGAAGAAGATTGAGAGAGAAAGCGATCTTTTAGTAGCATTTTCAAAGAGAAAGTCTGGCATTTACAACAAAGCCTCTGAATTATCAACACTTTGTGGGGCTTTAGTTGATATTCTTATTATTTCTCCAGCAGGAAAACCATATTGTTATGGAGAACCTTCTTCTCAATCCATAGCAAGAACAATTTTGAAGAATGAAAACCCTTCAGTAGAAGAAGAATTAAAGCAACTAAAGATTAACAAAGTTAACACAAAAAATGATGAGCTTTTGGATGAAAAGCATGTTGCGGAAGCACAACGGGAAAGTTTGAAGGCTTTGGAAAGTTATGGCTATTGGTCGATCAATGAGAAAGATGTTGAAAGCTTTGATTATGACCAGTTAAAAAAATTGGACACATCTTTAGTGGACATTGCAAATAAATTGATAAGTAGTGTCTTAAGCAATGGAGGAGAGATTAATCCTCATGCAATTTCTTGCATAGACGAACTGAATGGAGTCCAGATCAATCCTCCTTATGTCTTTGGGGCCAACAACAACGGTTATGAAATTGGTGCAACTCATGGATCCCAGATTTATGATCCAAAAGGAAAAGGCAAGGATGTTATTGCTTCTCTTGAAGGTAGAAATAAAACACAAGGACCAAACCTTGACCGTGCAAGTGAAAGTGGAAATGGAAGTGATGTTGATCATTCTCCTAAAGATGAATGA
Protein sequence

>Msa0549060

MAPKRTSRGRQKREMKKIERESDLLVAFSKRKSGIYNKASELSTLCGALVDILIISPAGKPYCYGEPSSQSIARTILKNENPSVEEELKQLKINKVNTKNDELLDEKHVAEAQRESLKALESYGYWSINEKDVESFDYDQLKKLDTSLVDIANKLISSVLSNGGEINPHAISCIDELNGVQINPPYVFGANNNGYEIGATHGSQIYDPKGKGKDVIASLEGRNKTQGPNLDRASESGNGSDVDHSPKDE*