AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0549210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549210 MtrunA17_Chr2g0332121 85.214 257 37 1 1 257 1 256 5.05e-149 422
Msa0549210 MtrunA17_Chr4g0002411 87.097 155 19 1 82 235 18 172 9.48e-85 251
Msa0549210 MtrunA17_Chr3g0133791 41.739 230 125 5 9 236 5 227 6.31e-48 159
Msa0549210 MtrunA17_Chr8g0375001 45.270 148 70 2 115 256 14 156 9.10e-36 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0549210 AT5G58280.2 43.307 254 114 4 12 238 5 255 7.59e-59 189
Msa0549210 AT5G58280.3 43.922 255 110 5 12 238 5 254 4.28e-58 187
Msa0549210 AT5G58280.1 43.421 228 118 4 12 238 5 222 3.31e-52 171
Msa0549210 AT3G19184.2 39.841 251 126 4 12 254 8 241 8.59e-47 158
Msa0549210 AT3G19184.1 39.841 251 126 4 12 254 8 241 1.10e-46 157
Msa0549210 AT5G42700.1 38.865 229 120 5 10 233 7 220 1.86e-42 145
Msa0549210 AT5G42700.2 38.122 181 99 3 55 233 6 175 1.12e-33 121

Find 76 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACATTAGTCTTTGATTT+TGG 0.195804 4_1:-44224657 None:intergenic
ATACGAAGGAACTGGGTTTC+TGG 0.220802 4_1:-44224706 None:intergenic
GGTTGAAGCAGCAAGATTTA+AGG 0.234558 4_1:+44225685 Msa0549210:CDS
TTCCTTTGATTTAACAGTTT+TGG 0.240573 4_1:-44227074 None:intergenic
TCGAGGTGTGCATCCTCTTT+TGG 0.268589 4_1:-44227166 None:intergenic
TTTAGATTCTTTGATTGCTT+TGG 0.273612 4_1:-44226975 None:intergenic
TGCGATTTCAGTCAATGTTT+TGG 0.279142 4_1:-44224513 None:intergenic
TTGGACTTTGGCTCAAGTTT+AGG 0.284818 4_1:-44227055 None:intergenic
AGCCGCTTCATACTTGTTAC+TGG 0.292196 4_1:-44223580 None:intergenic
TTAAATCAAAGGAAGCTTAT+AGG 0.293006 4_1:+44227083 Msa0549210:three_prime_UTR
TTTACCTTGGATTTCTTTGC+TGG 0.294171 4_1:-44224539 None:intergenic
TCGAAGACTGCAAATTGATT+TGG 0.297643 4_1:-44225301 None:intergenic
GAAGATTCTTGTTAAAATAA+TGG 0.308039 4_1:+44227285 Msa0549210:three_prime_UTR
TTCCTAAATCATCTCTTAGA+AGG 0.308163 4_1:-44227556 None:intergenic
GAAGGTGATGTATGTGAAAT+TGG 0.313037 4_1:+44227244 Msa0549210:three_prime_UTR
ATCAAAGACTAATGTTGTTC+TGG 0.324841 4_1:+44224662 Msa0549210:CDS
TCCCTTCTAAGAGATGATTT+AGG 0.349094 4_1:+44227554 Msa0549210:three_prime_UTR
GGCAACTTTGTAGCTGCCTC+TGG 0.368043 4_1:-44227199 None:intergenic
AGCTGAAGAGATTAAACAAA+AGG 0.375290 4_1:-44223186 None:intergenic
CATGTTCTTTCTTCTTGCCT+AGG 0.381909 4_1:-44227315 None:intergenic
AAAAGAAGAAAGTGATGCTT+TGG 0.395877 4_1:+44226926 Msa0549210:CDS
TCAGAAAGAGAGGTTAATCT+TGG 0.396784 4_1:+44227437 Msa0549210:three_prime_UTR
ATTTGCATTGAAACATAAAC+TGG 0.400811 4_1:+44225634 Msa0549210:CDS
CAAGAAGAAAGAACATGTTC+AGG 0.404310 4_1:+44227321 Msa0549210:three_prime_UTR
GGTTGTTATGAAATACTGAT+TGG 0.404992 4_1:+44227501 Msa0549210:three_prime_UTR
TCTTCACGATACGAAGGAAC+TGG 0.423062 4_1:-44224714 None:intergenic
CACTTATCAAGTCAATGGTT+TGG 0.424323 4_1:+44225330 Msa0549210:CDS
TTCATACTTGTTACTGGAAG+TGG 0.425368 4_1:-44223574 None:intergenic
TCAAGAAAGAAAGCGTGCTT+TGG 0.426683 4_1:+44225262 Msa0549210:CDS
CCTCTGGTTTCACAGTTTCG+AGG 0.431144 4_1:-44227183 None:intergenic
TTTCTTTCTCTACTCAATCA+AGG 0.439063 4_1:+44223223 Msa0549210:five_prime_UTR
GTTTGGTCTCACATTTATAG+AGG 0.450522 4_1:+44225347 Msa0549210:CDS
ACCTTCCACAGTTGCGGAAG+AGG 0.453437 4_1:+44225072 Msa0549210:CDS
TCCATCACTTATCAAGTCAA+TGG 0.461188 4_1:+44225325 Msa0549210:CDS
CAAGGTCAAGTTCATCATCA+TGG 0.471764 4_1:+44225096 Msa0549210:CDS
ACCTCTTCCGCAACTGTGGA+AGG 0.475917 4_1:-44225073 None:intergenic
GGAAAACAAGAAGAGGTTTG+AGG 0.478514 4_1:+44223620 Msa0549210:CDS
GCTGAAGAGATTAAACAAAA+GGG 0.487935 4_1:-44223185 None:intergenic
AAAGAAAACTAGAATGCAAA+AGG 0.490045 4_1:+44227021 Msa0549210:three_prime_UTR
AGAAGAAAGTGATGCTTTGG+TGG 0.490772 4_1:+44226929 Msa0549210:CDS
ACCATTGACTTGATAAGTGA+TGG 0.497922 4_1:-44225326 None:intergenic
ATTTGAAAATATAGATGAAG+AGG 0.499410 4_1:+44226899 Msa0549210:CDS
CTAATGTTGTTCTGGAACCT+AGG 0.501598 4_1:+44224670 Msa0549210:CDS
AAGGTCAAGTTCATCATCAT+GGG 0.503820 4_1:+44225097 Msa0549210:CDS
TGTTCCTCGCAAAATCTCCT+TGG 0.505957 4_1:-44225506 None:intergenic
AGTGATGCTTTGGTGGAAGA+AGG 0.506334 4_1:+44226936 Msa0549210:CDS
AGCAAGAAAACTGCGCTTAG+TGG 0.510768 4_1:+44225599 Msa0549210:CDS
AAACTGCGCTTAGTGGTGGC+TGG 0.513224 4_1:+44225606 Msa0549210:CDS
GTCCAAAACTGTTAAATCAA+AGG 0.523876 4_1:+44227072 Msa0549210:three_prime_UTR
TAGAATTACGATGTTAGTTG+AGG 0.529300 4_1:+44227637 Msa0549210:three_prime_UTR
CACAGTTGCGGAAGAGGTCA+AGG 0.544125 4_1:+44225078 Msa0549210:CDS
TACTGCAGTTGAGCCAAAAG+AGG 0.549920 4_1:+44227153 Msa0549210:three_prime_UTR
AAAATAATGGAGGCTGACCT+AGG 0.559343 4_1:+44227298 Msa0549210:three_prime_UTR
GCACAGACCTTCCACAGTTG+CGG 0.564774 4_1:+44225066 Msa0549210:CDS
GAGAGGTTAATCTTGGAGAC+CGG 0.565166 4_1:+44227444 Msa0549210:three_prime_UTR
GCGTGCTTTGGATGCTGCAG+AGG 0.570639 4_1:+44225274 Msa0549210:CDS
CTTGACCTCTTCCGCAACTG+TGG 0.570829 4_1:-44225077 None:intergenic
TCCTAAATCATCTCTTAGAA+GGG 0.582142 4_1:-44227555 None:intergenic
TTGAAACATAAACTGGATGA+TGG 0.585314 4_1:+44225641 Msa0549210:CDS
CTTCACGATACGAAGGAACT+GGG 0.585948 4_1:-44224713 None:intergenic
TCAGTTACGCTTACGTTGCG+AGG 0.594424 4_1:+44225210 Msa0549210:intron
GTGTGTAAGCAGATGGCAAA+CGG 0.598841 4_1:+44223546 Msa0549210:intron
GACTTGAGGAAAACAAGAAG+AGG 0.599382 4_1:+44223613 Msa0549210:CDS
CAAGTTCATCATCATGGGGA+AGG 0.599718 4_1:+44225102 Msa0549210:CDS
CTGGCACGAGAAGAGCGCCT+AGG 0.600809 4_1:-44224687 None:intergenic
AGTTCCTTCGTATCGTGAAG+AGG 0.609998 4_1:+44224716 Msa0549210:CDS
AAGAAAACTGCGCTTAGTGG+TGG 0.610549 4_1:+44225602 Msa0549210:CDS
TTCCAGTAACAAGTATGAAG+CGG 0.611515 4_1:+44223578 Msa0549210:CDS
ATCTAAAACTGTGAAACCAA+AGG 0.619114 4_1:+44226991 Msa0549210:exon
GATTCTTGTTAAAATAATGG+AGG 0.623792 4_1:+44227288 Msa0549210:three_prime_UTR
AAGTCCAGCAAAGAAATCCA+AGG 0.643226 4_1:+44224535 Msa0549210:CDS
AATTGGTGACTCACGTAATG+CGG 0.648633 4_1:+44227261 Msa0549210:three_prime_UTR
GGCTAGAGAACAAAGACTTG+AGG 0.682861 4_1:+44223599 Msa0549210:CDS
CTTACCTCTTCACGATACGA+AGG 0.683573 4_1:-44224720 None:intergenic
AGGTCAAGTTCATCATCATG+GGG 0.691621 4_1:+44225098 Msa0549210:CDS
CCTCGAAACTGTGAAACCAG+AGG 0.716116 4_1:+44227183 Msa0549210:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAACTTACAATTTTTTAAAT+CGG + chr4_1:44224559-44224578 Msa0549210:intron 10.0%
!!! ATATATTATCTTTATTTTGT+AGG + chr4_1:44225478-44225497 Msa0549210:intron 10.0%
!!! TATATTATCTTTATTTTGTA+GGG + chr4_1:44225479-44225498 Msa0549210:intron 10.0%
!! GATATTAAAATATTACAAAA+AGG - chr4_1:44225996-44226015 None:intergenic 10.0%
!! GTAAAATATAAATTTATTGA+AGG + chr4_1:44226556-44226575 Msa0549210:intron 10.0%
!! TCTTTAACAAAAAAAAAACA+GGG - chr4_1:44223316-44223335 None:intergenic 15.0%
!! TTCTTTAACAAAAAAAAAAC+AGG - chr4_1:44223317-44223336 None:intergenic 15.0%
!!! AAAATCATAAAAAACTTTTG+AGG + chr4_1:44224308-44224327 Msa0549210:intron 15.0%
!!! AAATTGAAATTTTATTGACA+TGG + chr4_1:44225176-44225195 Msa0549210:intron 15.0%
!! AATTTATTGAAGGTTAATTT+AGG + chr4_1:44226566-44226585 Msa0549210:intron 15.0%
!! CATATGAATATAATTATGTT+AGG - chr4_1:44226826-44226845 None:intergenic 15.0%
!! CTAACATAATTATATTCATA+TGG + chr4_1:44226824-44226843 Msa0549210:intron 15.0%
!!! TTTAAGCTTTTTCATTTCTT+GGG - chr4_1:44223365-44223384 None:intergenic 20.0%
!! TAAAATTATCTCAAACAAAG+TGG - chr4_1:44223652-44223671 None:intergenic 20.0%
!! TTTCATGTTAATTGAAATCA+AGG + chr4_1:44223752-44223771 Msa0549210:intron 20.0%
!!! AAATCAAGGTATTTAAATAG+TGG + chr4_1:44223766-44223785 Msa0549210:intron 20.0%
!! TTGCTTTATACTTTATATCA+TGG + chr4_1:44224198-44224217 Msa0549210:intron 20.0%
!! AAAAAATTGTAAGTTTACCT+TGG - chr4_1:44224555-44224574 None:intergenic 20.0%
!! TATTTCACTTTATTACAGTT+AGG + chr4_1:44225044-44225063 Msa0549210:intron 20.0%
!!! AAATTTTATTGACATGGTTA+TGG + chr4_1:44225182-44225201 Msa0549210:intron 20.0%
!!! AATTTTATTGACATGGTTAT+GGG + chr4_1:44225183-44225202 Msa0549210:intron 20.0%
!! TTGTTCATTCTTTAATGTTT+CGG + chr4_1:44225415-44225434 Msa0549210:intron 20.0%
!! TCCAAAAATTGACTATAAAA+TGG + chr4_1:44225532-44225551 Msa0549210:CDS 20.0%
!!! ACCATTTTATAGTCAATTTT+TGG - chr4_1:44225536-44225555 None:intergenic 20.0%
!!! ACCATTTTAGAATTTGAAAA+GGG - chr4_1:44226217-44226236 None:intergenic 20.0%
!!! TACCATTTTAGAATTTGAAA+AGG - chr4_1:44226218-44226237 None:intergenic 20.0%
!!! ATTAGTTTGAGAAACTTAAA+TGG - chr4_1:44226469-44226488 None:intergenic 20.0%
!!! ATAATTCTTTTTGTTTCTAC+TGG - chr4_1:44226772-44226791 None:intergenic 20.0%
!! ATTTGAAAATATAGATGAAG+AGG + chr4_1:44226899-44226918 Msa0549210:CDS 20.0%
!! GAAGATTCTTGTTAAAATAA+TGG + chr4_1:44227285-44227304 Msa0549210:three_prime_UTR 20.0%
!!! AAATACTGATATGCATTTTA+AGG + chr4_1:44227480-44227499 Msa0549210:three_prime_UTR 20.0%
!!! AAACTGTTTCTTTTACATAC+TGG + chr4_1:44223256-44223275 Msa0549210:intron 25.0%
!!! AACTGTTTCTTTTACATACT+GGG + chr4_1:44223257-44223276 Msa0549210:intron 25.0%
!!! CTTTAAGCTTTTTCATTTCT+TGG - chr4_1:44223366-44223385 None:intergenic 25.0%
!!! AAAGTTTTAAGCCATCATTA+AGG - chr4_1:44223697-44223716 None:intergenic 25.0%
! TATATATGCATGTGTATCTA+AGG + chr4_1:44223727-44223746 Msa0549210:intron 25.0%
! AAAGAAATAGTTGTCAAATG+TGG + chr4_1:44223811-44223830 Msa0549210:intron 25.0%
! TTTATACATACTACTCAATC+TGG - chr4_1:44223921-44223940 None:intergenic 25.0%
!!! TAAGTCATGGTTTTAAATTG+TGG + chr4_1:44224011-44224030 Msa0549210:intron 25.0%
! CTATATATACTGCTCAATAT+AGG - chr4_1:44224126-44224145 None:intergenic 25.0%
! TATATCATGGCTATAAATTG+TGG + chr4_1:44224211-44224230 Msa0549210:intron 25.0%
!!! ACAACATTAGTCTTTGATTT+TGG - chr4_1:44224660-44224679 None:intergenic 25.0%
! ATAGTGCAAAAATATCTACT+AGG + chr4_1:44224841-44224860 Msa0549210:intron 25.0%
! AATATCTACTAGGAATATGT+TGG + chr4_1:44224851-44224870 Msa0549210:intron 25.0%
!! ATTCTCATACTTTTGTCTTA+GGG - chr4_1:44224890-44224909 None:intergenic 25.0%
!! AATTCTCATACTTTTGTCTT+AGG - chr4_1:44224891-44224910 None:intergenic 25.0%
! ATCTTCAAATTCAACAATCA+AGG - chr4_1:44224970-44224989 None:intergenic 25.0%
! ACATTAAAGAATGAACAACA+AGG - chr4_1:44225413-44225432 None:intergenic 25.0%
!!! TGACTATAAAATGGTTTTAG+AGG + chr4_1:44225541-44225560 Msa0549210:CDS 25.0%
! ATTTGCATTGAAACATAAAC+TGG + chr4_1:44225634-44225653 Msa0549210:CDS 25.0%
!! TTTTCCTCAAAAACAAATCA+TGG - chr4_1:44225871-44225890 None:intergenic 25.0%
!!! ATGATTTGTTTTTGAGGAAA+AGG + chr4_1:44225870-44225889 Msa0549210:intron 25.0%
! GGAAAAGGAATAATTTCAAA+TGG + chr4_1:44225885-44225904 Msa0549210:intron 25.0%
! AAGAAGAAACTCAATATCAA+AGG - chr4_1:44225958-44225977 None:intergenic 25.0%
! TGACCAAATTAATTCTTACA+AGG + chr4_1:44226044-44226063 Msa0549210:intron 25.0%
!! AAGCCTTGTAAGAATTAATT+TGG - chr4_1:44226050-44226069 None:intergenic 25.0%
!! CAATTACTATATTTGGAACT+TGG - chr4_1:44226125-44226144 None:intergenic 25.0%
! CAGCTAACAATTACTATATT+TGG - chr4_1:44226132-44226151 None:intergenic 25.0%
! TATCTTCAAAAGTTATCTCA+TGG - chr4_1:44226173-44226192 None:intergenic 25.0%
!!! ACCCTTTTCAAATTCTAAAA+TGG + chr4_1:44226213-44226232 Msa0549210:intron 25.0%
!! CCTACTTATAACAACTATTT+TGG - chr4_1:44226361-44226380 None:intergenic 25.0%
! CCAAAATAGTTGTTATAAGT+AGG + chr4_1:44226358-44226377 Msa0549210:intron 25.0%
!!! TACTTTTAGGGTTGAAATTA+AGG + chr4_1:44226417-44226436 Msa0549210:intron 25.0%
! AACAAAAAGAATTATATGCC+AGG + chr4_1:44226777-44226796 Msa0549210:intron 25.0%
! TTTAGATTCTTTGATTGCTT+TGG - chr4_1:44226978-44226997 None:intergenic 25.0%
! AAAGAAAACTAGAATGCAAA+AGG + chr4_1:44227021-44227040 Msa0549210:three_prime_UTR 25.0%
!!! TTCCTTTGATTTAACAGTTT+TGG - chr4_1:44227077-44227096 None:intergenic 25.0%
! TTAAATCAAAGGAAGCTTAT+AGG + chr4_1:44227083-44227102 Msa0549210:three_prime_UTR 25.0%
! GATTCTTGTTAAAATAATGG+AGG + chr4_1:44227288-44227307 Msa0549210:three_prime_UTR 25.0%
!!! TTTTGATAATTGTTGCAGAA+TGG + chr4_1:44227594-44227613 Msa0549210:three_prime_UTR 25.0%
GCTGAAGAGATTAAACAAAA+GGG - chr4_1:44223188-44223207 None:intergenic 30.0%
AGCTGAAGAGATTAAACAAA+AGG - chr4_1:44223189-44223208 None:intergenic 30.0%
TTTCTTTCTCTACTCAATCA+AGG + chr4_1:44223223-44223242 Msa0549210:five_prime_UTR 30.0%
!! ACTGTTTCTTTTACATACTG+GGG + chr4_1:44223258-44223277 Msa0549210:intron 30.0%
! TTTTATGGAAAAAGAGTGAG+TGG + chr4_1:44223442-44223461 Msa0549210:intron 30.0%
TTTATGGAAAAAGAGTGAGT+GGG + chr4_1:44223443-44223462 Msa0549210:intron 30.0%
TCATTTGTGTACCTTAATGA+TGG + chr4_1:44223683-44223702 Msa0549210:intron 30.0%
AGAAACATCAAAAACCTTGA+TGG + chr4_1:44223859-44223878 Msa0549210:intron 30.0%
TTAAGCGATCTTTGATATCA+TGG + chr4_1:44224237-44224256 Msa0549210:intron 30.0%
! GGATAATAGTTGTGATAATG+AGG + chr4_1:44224345-44224364 Msa0549210:intron 30.0%
! GGTAGTTTGCATAAATCTAT+AGG - chr4_1:44224377-44224396 None:intergenic 30.0%
TAAATCCTTGAGAACAAAAG+GGG - chr4_1:44224477-44224496 None:intergenic 30.0%
CTAAATCCTTGAGAACAAAA+GGG - chr4_1:44224478-44224497 None:intergenic 30.0%
!! TAAATCGGTTTTACTCTGTA+AGG + chr4_1:44224574-44224593 Msa0549210:intron 30.0%
ATCAAAGACTAATGTTGTTC+TGG + chr4_1:44224662-44224681 Msa0549210:CDS 30.0%
GATATAGTTTGAACTTTGCA+AGG - chr4_1:44224754-44224773 None:intergenic 30.0%
ACTATCATGTTGGAAAAGAA+TGG - chr4_1:44224996-44225015 None:intergenic 30.0%
!!! GCTTTTTTGATTTCATTCAG+TGG - chr4_1:44225236-44225255 None:intergenic 30.0%
! CTCACATTTATAGAGGTTTT+TGG + chr4_1:44225354-44225373 Msa0549210:CDS 30.0%
!!! CATTTATAGAGGTTTTTGGT+TGG + chr4_1:44225358-44225377 Msa0549210:CDS 30.0%
!!! AATGGTTTTAGAGGATGAAA+AGG + chr4_1:44225550-44225569 Msa0549210:CDS 30.0%
!!! ATGGTTTTAGAGGATGAAAA+GGG + chr4_1:44225551-44225570 Msa0549210:CDS 30.0%
TTGAAACATAAACTGGATGA+TGG + chr4_1:44225641-44225660 Msa0549210:CDS 30.0%
! TGATGCAGTAGTTTTTGAAT+TGG + chr4_1:44225664-44225683 Msa0549210:CDS 30.0%
!! CTTTGAAGTTAAAAGAGAGT+TGG - chr4_1:44225734-44225753 None:intergenic 30.0%
!!! CAACTCTCTTTTAACTTCAA+AGG + chr4_1:44225732-44225751 Msa0549210:intron 30.0%
GATAATAACTGATATCAGCT+TGG + chr4_1:44225793-44225812 Msa0549210:intron 30.0%
!!! TTGTCCATGATTTGTTTTTG+AGG + chr4_1:44225864-44225883 Msa0549210:intron 30.0%
AGAACATCTAAGTTCATTAG+TGG - chr4_1:44225915-44225934 None:intergenic 30.0%
TATAGTAATTGTTAGCTGTG+AGG + chr4_1:44226134-44226153 Msa0549210:intron 30.0%
ATAGTAATTGTTAGCTGTGA+GGG + chr4_1:44226135-44226154 Msa0549210:intron 30.0%
!! ACTTTTGAAGATAGCTTTTG+TGG + chr4_1:44226180-44226199 Msa0549210:intron 30.0%
!! CTTTTGAAGATAGCTTTTGT+GGG + chr4_1:44226181-44226200 Msa0549210:intron 30.0%
GACATATCACTAGTAAATGT+GGG - chr4_1:44226339-44226358 None:intergenic 30.0%
!!! AGCTTTTGGAGTTTACTTTT+AGG + chr4_1:44226404-44226423 Msa0549210:intron 30.0%
!!! GCTTTTGGAGTTTACTTTTA+GGG + chr4_1:44226405-44226424 Msa0549210:intron 30.0%
! GGAAACTTGTTCTATAAGAA+AGG + chr4_1:44226527-44226546 Msa0549210:intron 30.0%
TTTGCAACCAAAAAACAAGT+GGG - chr4_1:44226695-44226714 None:intergenic 30.0%
ATTTGCAACCAAAAAACAAG+TGG - chr4_1:44226696-44226715 None:intergenic 30.0%
AAAAAGAATTATATGCCAGG+TGG + chr4_1:44226780-44226799 Msa0549210:intron 30.0%
AAAAGAAGAAAGTGATGCTT+TGG + chr4_1:44226926-44226945 Msa0549210:CDS 30.0%
! ATCTAAAACTGTGAAACCAA+AGG + chr4_1:44226991-44227010 Msa0549210:exon 30.0%
!!! GATTTAACAGTTTTGGACTT+TGG - chr4_1:44227070-44227089 None:intergenic 30.0%
GTCCAAAACTGTTAAATCAA+AGG + chr4_1:44227072-44227091 Msa0549210:three_prime_UTR 30.0%
! TTTGCCTTCTTTTTCTTGTT+TGG - chr4_1:44227223-44227242 None:intergenic 30.0%
AAGAAAAAGAAGGCAAAAGA+AGG + chr4_1:44227226-44227245 Msa0549210:three_prime_UTR 30.0%
! GTATTTCGCAATCTTTTAAC+CGG - chr4_1:44227466-44227485 None:intergenic 30.0%
GGTTGTTATGAAATACTGAT+TGG + chr4_1:44227501-44227520 Msa0549210:three_prime_UTR 30.0%
TCCTAAATCATCTCTTAGAA+GGG - chr4_1:44227558-44227577 None:intergenic 30.0%
TTCCTAAATCATCTCTTAGA+AGG - chr4_1:44227559-44227578 None:intergenic 30.0%
TAGAATTACGATGTTAGTTG+AGG + chr4_1:44227637-44227656 Msa0549210:three_prime_UTR 30.0%
AATCATTGTGTGTAAGCAGA+TGG + chr4_1:44223539-44223558 Msa0549210:intron 35.0%
! TTCATACTTGTTACTGGAAG+TGG - chr4_1:44223577-44223596 None:intergenic 35.0%
TTCCAGTAACAAGTATGAAG+CGG + chr4_1:44223578-44223597 Msa0549210:CDS 35.0%
! ATAACCCGCAACAACATTTT+AGG - chr4_1:44223890-44223909 None:intergenic 35.0%
AGAATTCAAAACTTCCGTGT+TGG + chr4_1:44223986-44224005 Msa0549210:intron 35.0%
AACCATGACTTAAACCAACA+CGG - chr4_1:44224003-44224022 None:intergenic 35.0%
!! GTTTTAAATTGTGGTCGAGT+CGG + chr4_1:44224020-44224039 Msa0549210:intron 35.0%
! TTTTAAATTGTGGTCGAGTC+GGG + chr4_1:44224021-44224040 Msa0549210:intron 35.0%
CAACTATGTTGGCAATATCA+AGG - chr4_1:44224049-44224068 None:intergenic 35.0%
TCACAACTATTATCCACATG+TGG - chr4_1:44224340-44224359 None:intergenic 35.0%
! AATATCCCCTTTTGTTCTCA+AGG + chr4_1:44224469-44224488 Msa0549210:intron 35.0%
CCTAAATCCTTGAGAACAAA+AGG - chr4_1:44224479-44224498 None:intergenic 35.0%
! CCTTTTGTTCTCAAGGATTT+AGG + chr4_1:44224476-44224495 Msa0549210:intron 35.0%
! TGCGATTTCAGTCAATGTTT+TGG - chr4_1:44224516-44224535 None:intergenic 35.0%
TTTACCTTGGATTTCTTTGC+TGG - chr4_1:44224542-44224561 None:intergenic 35.0%
CGATTCTGCACAAAGAAATA+GGG - chr4_1:44224627-44224646 None:intergenic 35.0%
ACGATTCTGCACAAAGAAAT+AGG - chr4_1:44224628-44224647 None:intergenic 35.0%
CTCAAGAAAGAGGAATATGT+TGG + chr4_1:44224810-44224829 Msa0549210:intron 35.0%
!!! ATTTAGCTCTAGAGTTTTGC+AGG + chr4_1:44224908-44224927 Msa0549210:intron 35.0%
AGAGAAAAATCACACAACAC+TGG - chr4_1:44224934-44224953 None:intergenic 35.0%
AACAATCAAGGATTTCTCGA+AGG - chr4_1:44224958-44224977 None:intergenic 35.0%
!! TTCCAACATGATAGTTTTGC+AGG + chr4_1:44225001-44225020 Msa0549210:intron 35.0%
TTCCTGCAAAACTATCATGT+TGG - chr4_1:44225006-44225025 None:intergenic 35.0%
AAGGTCAAGTTCATCATCAT+GGG + chr4_1:44225097-44225116 Msa0549210:CDS 35.0%
TCGAAGACTGCAAATTGATT+TGG - chr4_1:44225304-44225323 None:intergenic 35.0%
TCCATCACTTATCAAGTCAA+TGG + chr4_1:44225325-44225344 Msa0549210:CDS 35.0%
!! ACCATTGACTTGATAAGTGA+TGG - chr4_1:44225329-44225348 None:intergenic 35.0%
CACTTATCAAGTCAATGGTT+TGG + chr4_1:44225330-44225349 Msa0549210:CDS 35.0%
GTTTGGTCTCACATTTATAG+AGG + chr4_1:44225347-44225366 Msa0549210:CDS 35.0%
!!! TATAGAGGTTTTTGGTTGGT+AGG + chr4_1:44225362-44225381 Msa0549210:intron 35.0%
!! TTATTTTGTAGGGTCTTCCA+AGG + chr4_1:44225489-44225508 Msa0549210:intron 35.0%
AAAGAGAGTTGGATGAGAAT+TGG - chr4_1:44225723-44225742 None:intergenic 35.0%
TGAGTTTCTTCTTGAGTTCA+AGG + chr4_1:44225965-44225984 Msa0549210:intron 35.0%
TAGTAATTGTTAGCTGTGAG+GGG + chr4_1:44226136-44226155 Msa0549210:intron 35.0%
GGACATATCACTAGTAAATG+TGG - chr4_1:44226340-44226359 None:intergenic 35.0%
TATAGAACAAGTTTCCCTGA+GGG - chr4_1:44226523-44226542 None:intergenic 35.0%
TTATAGAACAAGTTTCCCTG+AGG - chr4_1:44226524-44226543 None:intergenic 35.0%
! GTTTTCTTTGTAGCTTCCTT+TGG - chr4_1:44227010-44227029 None:intergenic 35.0%
GTTGCCAAACAAGAAAAAGA+AGG + chr4_1:44227216-44227235 Msa0549210:three_prime_UTR 35.0%
GAAGGTGATGTATGTGAAAT+TGG + chr4_1:44227244-44227263 Msa0549210:three_prime_UTR 35.0%
CAAGAAGAAAGAACATGTTC+AGG + chr4_1:44227321-44227340 Msa0549210:three_prime_UTR 35.0%
! CCTCTCTTTCTGAAAAACTT+CGG - chr4_1:44227430-44227449 None:intergenic 35.0%
TCAGAAAGAGAGGTTAATCT+TGG + chr4_1:44227437-44227456 Msa0549210:three_prime_UTR 35.0%
! TCCCTTCTAAGAGATGATTT+AGG + chr4_1:44227554-44227573 Msa0549210:three_prime_UTR 35.0%
! CATACTGGGGTTACTTGTTA+TGG + chr4_1:44223271-44223290 Msa0549210:intron 40.0%
GACTTGAGGAAAACAAGAAG+AGG + chr4_1:44223613-44223632 Msa0549210:CDS 40.0%
GGAAAACAAGAAGAGGTTTG+AGG + chr4_1:44223620-44223639 Msa0549210:CDS 40.0%
! GTTGTCAAATGTGGTTGATG+TGG + chr4_1:44223820-44223839 Msa0549210:intron 40.0%
!! CATCAAAAACCTTGATGGTG+TGG + chr4_1:44223864-44223883 Msa0549210:intron 40.0%
TGTGGCCTAAAATGTTGTTG+CGG + chr4_1:44223882-44223901 Msa0549210:intron 40.0%
!! TTCCGTGTTGGTTTAAGTCA+TGG + chr4_1:44223998-44224017 Msa0549210:intron 40.0%
GCCAACATAGTTGTCAACTT+TGG + chr4_1:44224056-44224075 Msa0549210:intron 40.0%
GCCAAAGTTGACAACTATGT+TGG - chr4_1:44224060-44224079 None:intergenic 40.0%
AAGTCCAGCAAAGAAATCCA+AGG + chr4_1:44224535-44224554 Msa0549210:CDS 40.0%
!! CTAATGTTGTTCTGGAACCT+AGG + chr4_1:44224670-44224689 Msa0549210:CDS 40.0%
GTAACTCTGACTCAAGAAAG+AGG + chr4_1:44224800-44224819 Msa0549210:intron 40.0%
CAAGGTCAAGTTCATCATCA+TGG + chr4_1:44225096-44225115 Msa0549210:CDS 40.0%
AGGTCAAGTTCATCATCATG+GGG + chr4_1:44225098-44225117 Msa0549210:CDS 40.0%
! TCAAGAAAGAAAGCGTGCTT+TGG + chr4_1:44225262-44225281 Msa0549210:CDS 40.0%
! TAAGCGCAGTTTTCTTGCTA+AGG - chr4_1:44225598-44225617 None:intergenic 40.0%
GGTTGAAGCAGCAAGATTTA+AGG + chr4_1:44225685-44225704 Msa0549210:CDS 40.0%
! TCTATCACTATCGACTGATC+GGG - chr4_1:44225770-44225789 None:intergenic 40.0%
! GTCTATCACTATCGACTGAT+CGG - chr4_1:44225771-44225790 None:intergenic 40.0%
ATCAGTCGATAGTGATAGAC+AGG + chr4_1:44225771-44225790 Msa0549210:intron 40.0%
! TATCAGCTTGGTGAGAATGT+TGG + chr4_1:44225805-44225824 Msa0549210:intron 40.0%
! TTGGTGAGAATGTTGGTTGA+TGG + chr4_1:44225812-44225831 Msa0549210:intron 40.0%
!!! CTAGCTTTTGAGGTTGAGTT+AGG + chr4_1:44226099-44226118 Msa0549210:intron 40.0%
! TATTTTCCAAATTGCCCCTC+AGG + chr4_1:44226505-44226524 Msa0549210:intron 40.0%
! ATTTTCCAAATTGCCCCTCA+GGG + chr4_1:44226506-44226525 Msa0549210:intron 40.0%
ATAGAACAAGTTTCCCTGAG+GGG - chr4_1:44226522-44226541 None:intergenic 40.0%
!! GCAATGTTCTCTAAAACTGC+AGG + chr4_1:44226664-44226683 Msa0549210:intron 40.0%
AACCAAAAAACAAGTGGGTC+TGG - chr4_1:44226690-44226709 None:intergenic 40.0%
ATATCATCATTCGATCCACC+TGG - chr4_1:44226798-44226817 None:intergenic 40.0%
AGAAGAAAGTGATGCTTTGG+TGG + chr4_1:44226929-44226948 Msa0549210:CDS 40.0%
! TTGGACTTTGGCTCAAGTTT+AGG - chr4_1:44227058-44227077 None:intergenic 40.0%
! AATTGGTGACTCACGTAATG+CGG + chr4_1:44227261-44227280 Msa0549210:three_prime_UTR 40.0%
! AAAATAATGGAGGCTGACCT+AGG + chr4_1:44227298-44227317 Msa0549210:three_prime_UTR 40.0%
CATGTTCTTTCTTCTTGCCT+AGG - chr4_1:44227318-44227337 None:intergenic 40.0%
!! CCGAAGTTTTTCAGAAAGAG+AGG + chr4_1:44227427-44227446 Msa0549210:three_prime_UTR 40.0%
GTGTGTAAGCAGATGGCAAA+CGG + chr4_1:44223546-44223565 Msa0549210:intron 45.0%
! AGCCGCTTCATACTTGTTAC+TGG - chr4_1:44223583-44223602 None:intergenic 45.0%
GGCTAGAGAACAAAGACTTG+AGG + chr4_1:44223599-44223618 Msa0549210:CDS 45.0%
! GGTTGATGTGGAATCAATCG+TGG + chr4_1:44223832-44223851 Msa0549210:intron 45.0%
! CATTTTAGGCCACACCATCA+AGG - chr4_1:44223876-44223895 None:intergenic 45.0%
GTGGCCTAAAATGTTGTTGC+GGG + chr4_1:44223883-44223902 Msa0549210:intron 45.0%
!! GTTGTCAACTTTGGCTTTCG+TGG + chr4_1:44224065-44224084 Msa0549210:intron 45.0%
!! TCAACTTTGGCTTTCGTGGT+CGG + chr4_1:44224069-44224088 Msa0549210:intron 45.0%
AAAGACTACGCTAGTGGTGA+GGG - chr4_1:44224405-44224424 None:intergenic 45.0%
AGACACAAAGACTACGCTAG+TGG - chr4_1:44224411-44224430 None:intergenic 45.0%
ATACGAAGGAACTGGGTTTC+TGG - chr4_1:44224709-44224728 None:intergenic 45.0%
CTTCACGATACGAAGGAACT+GGG - chr4_1:44224716-44224735 None:intergenic 45.0%
TCTTCACGATACGAAGGAAC+TGG - chr4_1:44224717-44224736 None:intergenic 45.0%
AGTTCCTTCGTATCGTGAAG+AGG + chr4_1:44224716-44224735 Msa0549210:CDS 45.0%
CTTACCTCTTCACGATACGA+AGG - chr4_1:44224723-44224742 None:intergenic 45.0%
TCGTATCGTGAAGAGGTAAG+CGG + chr4_1:44224723-44224742 Msa0549210:intron 45.0%
CAAGTTCATCATCATGGGGA+AGG + chr4_1:44225102-44225121 Msa0549210:CDS 45.0%
! TCTTCCAAGGAGATTTTGCG+AGG + chr4_1:44225502-44225521 Msa0549210:CDS 45.0%
TGTTCCTCGCAAAATCTCCT+TGG - chr4_1:44225509-44225528 None:intergenic 45.0%
AGCAAGAAAACTGCGCTTAG+TGG + chr4_1:44225599-44225618 Msa0549210:CDS 45.0%
AAGAAAACTGCGCTTAGTGG+TGG + chr4_1:44225602-44225621 Msa0549210:CDS 45.0%
GGTCATATCTCTAGCTGATG+TGG - chr4_1:44226029-44226048 None:intergenic 45.0%
TCAACCTCAAAAGCTAGCAG+AGG - chr4_1:44226096-44226115 None:intergenic 45.0%
TCACACTCAAGACGAAGGTT+GGG - chr4_1:44226299-44226318 None:intergenic 45.0%
TTCACACTCAAGACGAAGGT+TGG - chr4_1:44226300-44226319 None:intergenic 45.0%
ACCTTCGTCTTGAGTGTGAA+AGG + chr4_1:44226300-44226319 Msa0549210:intron 45.0%
!!! TCTTCGTGTGAGCTAGCTTT+TGG + chr4_1:44226390-44226409 Msa0549210:intron 45.0%
TCCTGACAATTCATTCGTGC+AGG + chr4_1:44226860-44226879 Msa0549210:intron 45.0%
ACCTGCACGAATGAATTGTC+AGG - chr4_1:44226864-44226883 None:intergenic 45.0%
AGTGATGCTTTGGTGGAAGA+AGG + chr4_1:44226936-44226955 Msa0549210:CDS 45.0%
TACTGCAGTTGAGCCAAAAG+AGG + chr4_1:44227153-44227172 Msa0549210:three_prime_UTR 45.0%
AACCTGCTGCAGAAAAACCT+TGG + chr4_1:44227392-44227411 Msa0549210:three_prime_UTR 45.0%
! TTCCAAGGTTTTTCTGCAGC+AGG - chr4_1:44227397-44227416 None:intergenic 45.0%
!!! TTCGGAGCTCGTTTTTTCCA+AGG - chr4_1:44227412-44227431 None:intergenic 45.0%
GAGAGGTTAATCTTGGAGAC+CGG + chr4_1:44227444-44227463 Msa0549210:three_prime_UTR 45.0%
!!! TTAAAACTTTTTAAATTTTA+TGG + chr4_1:44223427-44223446 Msa0549210:intron 5.0%
! TTTGAGGTTGCAGCCACATG+TGG + chr4_1:44224324-44224343 Msa0549210:intron 50.0%
CAAAGACTACGCTAGTGGTG+AGG - chr4_1:44224406-44224425 None:intergenic 50.0%
GTTCATCATCATGGGGAAGG+TGG + chr4_1:44225105-44225124 Msa0549210:intron 50.0%
TCAGTTACGCTTACGTTGCG+AGG + chr4_1:44225210-44225229 Msa0549210:intron 50.0%
AGCAGAGGAGACAAGAGGTA+AGG - chr4_1:44226081-44226100 None:intergenic 50.0%
AAGCTAGCAGAGGAGACAAG+AGG - chr4_1:44226086-44226105 None:intergenic 50.0%
!!! GTCTCCTCTGCTAGCTTTTG+AGG + chr4_1:44226089-44226108 Msa0549210:intron 50.0%
TTTGCAGGTGGCGTGATAAG+TGG - chr4_1:44226273-44226292 None:intergenic 50.0%
ACTTATCACGCCACCTGCAA+AGG + chr4_1:44226272-44226291 Msa0549210:intron 50.0%
CACACTCAAGACGAAGGTTG+GGG - chr4_1:44226298-44226317 None:intergenic 50.0%
GCCTTTCACACTCAAGACGA+AGG - chr4_1:44226304-44226323 None:intergenic 50.0%
AGTTTCCCTGAGGGGCAATT+TGG - chr4_1:44226514-44226533 None:intergenic 50.0%
!!! GGCCAGACCCACTTGTTTTT+TGG + chr4_1:44226685-44226704 Msa0549210:intron 50.0%
! TCGAGGTGTGCATCCTCTTT+TGG - chr4_1:44227169-44227188 None:intergenic 50.0%
! CCTCTGGTTTCACAGTTTCG+AGG - chr4_1:44227186-44227205 None:intergenic 50.0%
CCTCGAAACTGTGAAACCAG+AGG + chr4_1:44227183-44227202 Msa0549210:three_prime_UTR 50.0%
GCACAGACCTTCCACAGTTG+CGG + chr4_1:44225066-44225085 Msa0549210:CDS 55.0%
ACCTTCCACAGTTGCGGAAG+AGG + chr4_1:44225072-44225091 Msa0549210:CDS 55.0%
ACCTCTTCCGCAACTGTGGA+AGG - chr4_1:44225076-44225095 None:intergenic 55.0%
CTTGACCTCTTCCGCAACTG+TGG - chr4_1:44225080-44225099 None:intergenic 55.0%
CACAGTTGCGGAAGAGGTCA+AGG + chr4_1:44225078-44225097 Msa0549210:CDS 55.0%
AAACTGCGCTTAGTGGTGGC+TGG + chr4_1:44225606-44225625 Msa0549210:CDS 55.0%
GGCAACTTTGTAGCTGCCTC+TGG - chr4_1:44227202-44227221 None:intergenic 55.0%
ACGCTAGTGGTGAGGGACAC+AGG - chr4_1:44224398-44224417 None:intergenic 60.0%
! GCGTGCTTTGGATGCTGCAG+AGG + chr4_1:44225274-44225293 Msa0549210:CDS 60.0%
GCAGGTGGCGTGATAAGTGG+TGG - chr4_1:44226270-44226289 None:intergenic 60.0%
AAGGTTGGGGCCTTTGCAGG+TGG - chr4_1:44226285-44226304 None:intergenic 60.0%
ACGAAGGTTGGGGCCTTTGC+AGG - chr4_1:44226288-44226307 None:intergenic 60.0%
!! CTGGCACGAGAAGAGCGCCT+AGG - chr4_1:44224690-44224709 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 44223172 44227673 44223172 ID=Msa0549210;Name=Msa0549210
chr4_1 mRNA 44223172 44227673 44223172 ID=Msa0549210-mRNA-1;Parent=Msa0549210;Name=Msa0549210-mRNA-1;_AED=0.09;_eAED=0.09;_QI=73|1|1|1|0.85|0.87|8|669|267
chr4_1 exon 44223172 44223244 44223172 ID=Msa0549210-mRNA-1:exon:11403;Parent=Msa0549210-mRNA-1
chr4_1 exon 44223558 44223641 44223558 ID=Msa0549210-mRNA-1:exon:11404;Parent=Msa0549210-mRNA-1
chr4_1 exon 44224491 44224556 44224491 ID=Msa0549210-mRNA-1:exon:11405;Parent=Msa0549210-mRNA-1
chr4_1 exon 44224642 44224737 44224642 ID=Msa0549210-mRNA-1:exon:11406;Parent=Msa0549210-mRNA-1
chr4_1 exon 44225062 44225123 44225062 ID=Msa0549210-mRNA-1:exon:11407;Parent=Msa0549210-mRNA-1
chr4_1 exon 44225214 44225379 44225214 ID=Msa0549210-mRNA-1:exon:11408;Parent=Msa0549210-mRNA-1
chr4_1 exon 44225500 44225706 44225500 ID=Msa0549210-mRNA-1:exon:11409;Parent=Msa0549210-mRNA-1
chr4_1 exon 44226882 44227673 44226882 ID=Msa0549210-mRNA-1:exon:11410;Parent=Msa0549210-mRNA-1
chr4_1 five_prime_UTR 44223172 44223244 44223172 ID=Msa0549210-mRNA-1:five_prime_utr;Parent=Msa0549210-mRNA-1
chr4_1 CDS 44223558 44223641 44223558 ID=Msa0549210-mRNA-1:cds;Parent=Msa0549210-mRNA-1
chr4_1 CDS 44224491 44224556 44224491 ID=Msa0549210-mRNA-1:cds;Parent=Msa0549210-mRNA-1
chr4_1 CDS 44224642 44224737 44224642 ID=Msa0549210-mRNA-1:cds;Parent=Msa0549210-mRNA-1
chr4_1 CDS 44225062 44225123 44225062 ID=Msa0549210-mRNA-1:cds;Parent=Msa0549210-mRNA-1
chr4_1 CDS 44225214 44225379 44225214 ID=Msa0549210-mRNA-1:cds;Parent=Msa0549210-mRNA-1
chr4_1 CDS 44225500 44225706 44225500 ID=Msa0549210-mRNA-1:cds;Parent=Msa0549210-mRNA-1
chr4_1 CDS 44226882 44227004 44226882 ID=Msa0549210-mRNA-1:cds;Parent=Msa0549210-mRNA-1
chr4_1 three_prime_UTR 44227005 44227673 44227005 ID=Msa0549210-mRNA-1:three_prime_utr;Parent=Msa0549210-mRNA-1
Gene Sequence

>Msa0549210

ATGGCAAACGGCTGTTCCACTTCCAGTAACAAGTATGAAGCGGCTAGAGAACAAAGACTTGAGGAAAACAAGAAGAGGTTTGAGGATTTAGGAATTTCAAAAATTTCCAAAACATTGACTGAAATCGCAAGTCCAGCAAAGAAATCCAAGAATCGTCTTCTCATACCAAAATCAAAGACTAATGTTGTTCTGGAACCTAGGCGCTCTTCTCGTGCCAGAAACCCAGTTCCTTCGTATCGTGAAGAGTTAGGCACAGACCTTCCACAGTTGCGGAAGAGGTCAAGGTCAAGTTCATCATCATGGGGAAGTTACGCTTACGTTGCGAGGCCACTGAATGAAATCAAAAAAGCTACAGATCAAGAAAGAAAGCGTGCTTTGGATGCTGCAGAGGCACTCCAAATCAATTTGCAGTCTTCGAATCCATCACTTATCAAGTCAATGGTTTGGTCTCACATTTATAGAGGTTTTTGGTTGGGTCTTCCAAGGAGATTTTGCGAGGAACATCTTCCAAAAATTGACTATAAAATGGTTTTAGAGGATGAAAAGGGATTAGAATATGATGTTGTTTACCTTAGCAAGAAAACTGCGCTTAGTGGTGGCTGGAGAGCATTTGCATTGAAACATAAACTGGATGATGGTGATGCAGTAGTTTTTGAATTGGTTGAAGCAGCAAGATTTAAGGTTTATATTGTTAGAGCATTTGAAAATATAGATGAAGAGGAAGAAAAAGAAGAAAGTGATGCTTTGGTGGAAGAAGGAAATATATGCACACATCCAAAGCAATCAAAGAATCTAAAACTGTGA
Protein sequence

>Msa0549210

MANGCSTSSNKYEAAREQRLEENKKRFEDLGISKISKTLTEIASPAKKSKNRLLIPKSKTNVVLEPRRSSRARNPVPSYREELGTDLPQLRKRSRSSSSSWGSYAYVARPLNEIKKATDQERKRALDAAEALQINLQSSNPSLIKSMVWSHIYRGFWLGLPRRFCEEHLPKIDYKMVLEDEKGLEYDVVYLSKKTALSGGWRAFALKHKLDDGDAVVFELVEAARFKVYIVRAFENIDEEEEKEESDALVEEGNICTHPKQSKNLKL*