AlfalfaGEDB | Msa0552130

M. sativa cultivar zhongmu-4 / Msa0552130

KEGG: -
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0552130	XP_024636999.1	88.328	317	29	1	96	412	140	448	0.0	567

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0552130	sp\|P93604\|LRK10_WHEAT	42.692	260	131	8	159	412	234	481	6.37e-60	208

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0552130	A0A396I9I0	88.328	317	29	1	96	412	140	448	0.0	567

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0552130	MtrunA17_Chr4g0037001	88.328	317	29	1	96	412	140	448	0.0	567
Msa0552130	MtrunA17_Chr1g0158771	48.640	331	146	7	83	412	122	429	6.07e-94	294
Msa0552130	MtrunA17_Chr1g0160771	50.162	309	135	5	109	412	147	441	1.82e-89	283
Msa0552130	MtrunA17_Chr1g0158651	48.896	317	137	5	105	412	147	447	2.45e-89	282
Msa0552130	MtrunA17_Chr1g0160731	49.669	302	138	6	116	412	145	437	2.79e-86	274
Msa0552130	MtrunA17_Chr1g0157781	48.097	289	144	3	124	412	161	443	5.40e-86	273
Msa0552130	MtrunA17_Chr1g0157941	52.128	282	109	5	132	412	143	399	5.70e-86	272
Msa0552130	MtrunA17_Chr1g0160581	46.372	317	151	6	106	412	138	445	1.18e-85	273
Msa0552130	MtrunA17_Chr1g0158181	44.880	332	166	7	86	412	147	466	2.25e-85	273
Msa0552130	MtrunA17_Chr1g0157801	44.340	318	166	4	95	412	132	438	1.49e-84	270

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0552130	AT1G66910.1	40.711	253	138	4	170	412	236	486	2.29e-51	183
Msa0552130	AT1G66910.2	41.107	253	134	6	170	412	236	483	1.24e-50	181
Msa0552130	AT1G66920.2	52.941	153	69	2	261	412	296	446	1.08e-47	172
Msa0552130	AT1G66920.1	52.941	153	69	2	261	412	288	438	1.10e-47	172
Msa0552130	AT1G66980.3	48.295	176	86	3	238	412	799	970	7.16e-47	173
Msa0552130	AT1G66980.1	48.295	176	86	3	238	412	772	943	7.49e-47	172
Msa0552130	AT1G66980.4	48.295	176	86	3	238	412	755	926	7.79e-47	172
Msa0552130	AT1G66980.2	48.295	176	86	3	238	412	801	972	7.81e-47	172
Msa0552130	AT5G38280.1	50.980	153	72	2	261	412	320	470	1.22e-46	170
Msa0552130	AT5G38280.2	50.980	153	72	2	261	412	320	470	1.48e-46	170

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Find 72 sgRNAs with CRISPR-Local

Find 322 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTATCAAACGAAGTTC+AGG	0.376535	4_1:-48559057	Msa0552130:CDS
AAATCAGTTTAAGGAAGAAC+TGG	0.364992	4_1:-48559111	Msa0552130:CDS
AAATTCTGCAACGAACCTTT+TGG	0.356760	4_1:+48558882	Msa0552110:intron
AAGAATCTCAAATCAGTTTA+AGG	0.204613	4_1:-48559120	Msa0552130:CDS
AAGAATTAGGCAACAATACA+AGG	0.527901	4_1:+48559666	Msa0552110:intron
AAGGTCTTGGACTCTGGTAC+AGG	0.517832	4_1:+48559685	Msa0552110:intron
AATCAGTTTAAGGAAGAACT+GGG	0.560574	4_1:-48559110	Msa0552130:CDS
AATTTCACACCCAAGCATTG+AGG	0.575791	4_1:-48558702	None:intergenic
ACGTTGATGCGGGTCTCAAT+TGG	0.461217	4_1:-48559631	Msa0552130:CDS
ACTCTGGTACAGGACAACAA+GGG	0.802635	4_1:+48559695	Msa0552110:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTATCATCTAATGTCAAAGT+TGG	-	chr4_1:48558734-48558753	Msa0552130:CDS	30.0%
ATCATCTAATGTCAAAGTTG+GGG	-	chr4_1:48558736-48558755	Msa0552130:CDS	30.0%
AATGTTTATCATTCACCTGT+TGG	-	chr4_1:48558919-48558938	Msa0552130:CDS	30.0%
ATGTTTATCATTCACCTGTT+GGG	-	chr4_1:48558920-48558939	Msa0552130:CDS	30.0%
CTTTAACCATCCAAATTGTA+AGG	-	chr4_1:48558961-48558980	Msa0552130:CDS	30.0%
TTAACCATCCAAATTGTAAG+GGG	-	chr4_1:48558963-48558982	Msa0552130:CDS	30.0%
CAATGATAAATCCAACAAAG+AGG	+	chr4_1:48559473-48559492	Msa0552110:intron	30.0%
TGTTGGATTTATCATTGTTG+AGG	-	chr4_1:48559476-48559495	Msa0552130:intron	30.0%
TAATCAGAGTAATCACACAA+CGG	+	chr4_1:48559675-48559694	Msa0552110:intron	30.0%
AAGAATTAGGCAACAATACA+AGG	+	chr4_1:48559911-48559930	Msa0552110:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	48558704	48560892	48558704	ID=Msa0552130;Name=Msa0552130
chr4_1	mRNA	48558704	48560892	48558704	ID=Msa0552130-mRNA-1;Parent=Msa0552130;Name=Msa0552130-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0\|0\|0\|0.4\|1\|1\|5\|0\|413
chr4_1	exon	48560782	48560892	48560782	ID=Msa0552130-mRNA-1:exon:13337;Parent=Msa0552130-mRNA-1
chr4_1	exon	48560418	48560589	48560418	ID=Msa0552130-mRNA-1:exon:13336;Parent=Msa0552130-mRNA-1
chr4_1	exon	48559531	48559842	48559531	ID=Msa0552130-mRNA-1:exon:13335;Parent=Msa0552130-mRNA-1
chr4_1	exon	48559388	48559438	48559388	ID=Msa0552130-mRNA-1:exon:13334;Parent=Msa0552130-mRNA-1
chr4_1	exon	48558704	48559299	48558704	ID=Msa0552130-mRNA-1:exon:13333;Parent=Msa0552130-mRNA-1
chr4_1	CDS	48560782	48560892	48560782	ID=Msa0552130-mRNA-1:cds;Parent=Msa0552130-mRNA-1
chr4_1	CDS	48560418	48560589	48560418	ID=Msa0552130-mRNA-1:cds;Parent=Msa0552130-mRNA-1
chr4_1	CDS	48559531	48559842	48559531	ID=Msa0552130-mRNA-1:cds;Parent=Msa0552130-mRNA-1
chr4_1	CDS	48559388	48559438	48559388	ID=Msa0552130-mRNA-1:cds;Parent=Msa0552130-mRNA-1
chr4_1	CDS	48558704	48559299	48558704	ID=Msa0552130-mRNA-1:cds;Parent=Msa0552130-mRNA-1

Gene Sequence

>Msa0552130

ATGTTGTTGATTTCCGTTGTTGACGTTGTTGTATCATCTAATGTCAAAGTTGGGGAGTGTGTCATAATTGTTTTAGGCACATATGTTGTTGTTGTAGTCGCTGTTGAAGTTCATTCAACGCGCGTAGATTGGTACCACCTGCATGATGCATTAGTTGTTGTTGTCGTAGCATTGTTGGGTTGTCGTTGTCGTTGTAGCATTGTCGAGTTGTTGTCGTTGTCGCATTGTCGAGTTGTCGGTGTCGTTGTGGATAATGTTGTTGTTGTCGTTGTTGCTGTACCTTGTGCTTCTCACAAAAAAACATATGATGGATATGATAATATATTAACATCTGGAAACTTTTCTTGTCTCAACGTGCCGGGATTTGAAGTTATAGCAGTCAATTCCATATCATCAATAATTCGGGCACCCTTGTTGTCCTGTACCAGAGTCCAAGACCTTGTATTGTTGCCTAATTCTTTGTTTAACAACAACGTTGATGCGGGTCTCAATTGGCTTCAACCATACTGCAGAAGCTGTGAGAGTAAAGGTGGGCAATGCAAGGCCAACATAGACACATCTCGACACGAATTTGAATGCACAGGCATCGGTAAAGGTTTATCAAAAGAGAAAGTAATTGCTGGAATGCTTTTCAAAGCCTCGTCTTGTGGAATCCTAGGCCCATTTTCTCTTATATTTGTTGGAGTCACAGTATATTGTATCTATGTAGCTTGCATTGAAAGAAAGAATTACATAAGAATTAAAAAGTTTTTGAAGGATTATAAAGCTCTTAAACCTTCCAGATACTCTTATGCAGATATAAAAAGAATCTCAAATCAGTTTAAGGAAGAACTGGGGAAGGGAGCTTTTGGATATGTCTTCAAAGGAAAACTATCAAACGAAGTTCAGGTAGCTGTTAAGGTCCTAAACACTTCAATAAGAAATGGCCAAGAGTTCATCAATGAAATGGAAGCTATGTGTCAAATTCATCATGTCAACGTGGCTCGCTTGGTTGGTTTTTGTGCAGACGGAAACAAACGAGCCTTGGTGTATGAATTTTTACCAAAAGGTTCGTTGCAGAATTTCATATCGTCTGCAGACAATAAGGATGTCTTTTTAGGTTGGGAGAGGCTTCAAAACATTTCTCTTGGTATACCGAAGGGAATTGAGTATCTTCATCAAGGTTGTGATAAACAAATTCTCCATTTTGATATCAAACCTCATAATGTATTGCTAGACGACAATTTCACACCCAAGCATTGA

Protein sequence

>Msa0552130

MLLISVVDVVVSSNVKVGECVIIVLGTYVVVVVAVEVHSTRVDWYHLHDALVVVVVALLGCRCRCSIVELLSLSHCRVVGVVVDNVVVVVVAVPCASHKKTYDGYDNILTSGNFSCLNVPGFEVIAVNSISSIIRAPLLSCTRVQDLVLLPNSLFNNNVDAGLNWLQPYCRSCESKGGQCKANIDTSRHEFECTGIGKGLSKEKVIAGMLFKASSCGILGPFSLIFVGVTVYCIYVACIERKNYIRIKKFLKDYKALKPSRYSYADIKRISNQFKEELGKGAFGYVFKGKLSNEVQVAVKVLNTSIRNGQEFINEMEAMCQIHHVNVARLVGFCADGNKRALVYEFLPKGSLQNFISSADNKDVFLGWERLQNISLGIPKGIEYLHQGCDKQILHFDIKPHNVLLDDNFTPKH*