AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0555360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555360 MtrunA17_Chr4g0041301 100.000 142 0 0 1 142 1 142 5.50e-101 285
Msa0555360 MtrunA17_Chr2g0320211 91.304 138 12 0 5 142 3 140 2.67e-89 256
Msa0555360 MtrunA17_Chr6g0485281 50.435 115 57 0 4 118 9 123 5.81e-34 115
Msa0555360 MtrunA17_Chr6g0457491 87.273 55 7 0 88 142 1 55 7.87e-28 97.8
Msa0555360 MtrunA17_Chr7g0275831 90.000 40 4 0 92 131 1 40 5.29e-19 75.5
Msa0555360 MtrunA17_Chr4g0057871 55.844 77 21 1 32 95 9 85 2.33e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0555360 AT3G58680.1 84.507 142 22 0 1 142 1 142 1.32e-86 249
Msa0555360 AT2G42680.1 85.915 142 20 0 1 142 1 142 1.77e-86 249
Msa0555360 AT3G24500.1 50.360 139 67 1 5 141 7 145 1.09e-43 141
Msa0555360 AT3G24500.2 43.820 89 48 1 5 91 7 95 4.84e-17 71.6

Find 71 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTCATTGGCCTGGTTTGC+AGG 0.172981 4_1:+53379690 Msa0555360:three_prime_UTR
ATTGGTGTTCAAGAATTAAT+TGG 0.217200 4_1:-53379717 None:intergenic
GACGGCTTTCTCATCTTTCT+TGG 0.250063 4_1:-53377207 None:intergenic
ATCTTTCTTGGCGGCGGCGT+TGG 0.250269 4_1:-53377195 None:intergenic
TTGCTAATTATCTGCTGGTT+TGG 0.292459 4_1:-53379396 None:intergenic
GTTCCCAATCTTGAGAAATA+TGG 0.320777 4_1:-53377148 None:intergenic
TAGCTCAGTGAAGGTGTAAA+TGG 0.330318 4_1:-53377059 None:intergenic
TTTCTTGACGGTATCGATAT+CGG 0.338092 4_1:-53377252 None:intergenic
TCTTTCTTGGCGGCGGCGTT+GGG 0.340591 4_1:-53377194 None:intergenic
GGCACGGCGAGCGGCGTTGA+CGG 0.351560 4_1:-53377225 None:intergenic
GTCAACGCCGCTCGCCGTGC+CGG 0.353656 4_1:+53377227 Msa0555360:CDS
CCATCATTGTGGCTGTTAGA+AGG 0.359290 4_1:+53379874 Msa0555360:three_prime_UTR
GGGTTTGCATGTCACTCTTA+AGG 0.362036 4_1:+53379487 Msa0555360:three_prime_UTR
TTGTGTTTATCTTGGTTCAT+TGG 0.367573 4_1:+53379677 Msa0555360:three_prime_UTR
GTATATGTAAAACATATAAA+TGG 0.380935 4_1:+53379542 Msa0555360:three_prime_UTR
TGACAAGTACTCTGCAAATC+AGG 0.389884 4_1:-53379781 None:intergenic
TAGGCCATATTTCTCAAGAT+TGG 0.393130 4_1:+53377144 Msa0555360:CDS
AGCAAGTTGGAGAGAGCCCT+TGG 0.404653 4_1:+53379414 Msa0555360:CDS
GTTCATTGGCCTGGTTTGCA+GGG 0.414765 4_1:+53379691 Msa0555360:three_prime_UTR
TTGGTGTTCAAGAATTAATT+GGG 0.418275 4_1:-53379716 None:intergenic
CCAACTTGCTAATTATCTGC+TGG 0.425113 4_1:-53379401 None:intergenic
GTGATCCAAGAGTACGAGTC+AGG 0.425391 4_1:+53379363 Msa0555360:CDS
CTTTCCCTGACTCGTACTCT+TGG 0.428066 4_1:-53379368 None:intergenic
AAGTTACAAATTTCAAACTT+AGG 0.430321 4_1:-53379751 None:intergenic
AGGCCATATTTCTCAAGATT+GGG 0.431239 4_1:+53377145 Msa0555360:CDS
ATTTCGCTTATTCGAACAAC+AGG 0.432195 4_1:-53379584 None:intergenic
TTCTTGAACACCAATGGACT+TGG 0.442586 4_1:+53379725 Msa0555360:three_prime_UTR
ATCGGCGCCGGCACGGCGAG+CGG 0.444792 4_1:-53377234 None:intergenic
GCTTTCTTGCGGATGACGAC+TGG 0.447489 4_1:-53377170 None:intergenic
CTGATTTCATGATTGTGCGC+TGG 0.453008 4_1:+53379466 Msa0555360:three_prime_UTR
GAGATAAATATGTCAGGTCT+AGG 0.459208 4_1:+53377125 Msa0555360:exon
AGTGAGTGTTGTGTTTATCT+TGG 0.461235 4_1:+53379669 Msa0555360:three_prime_UTR
AATTATCTGCTGGTTTGGAA+TGG 0.474147 4_1:-53379391 None:intergenic
CAGCGCACAATCATGAAATC+AGG 0.477136 4_1:-53379465 None:intergenic
TTTATCTTGGTTCATTGGCC+TGG 0.479258 4_1:+53379682 Msa0555360:three_prime_UTR
TGATTTCATGATTGTGCGCT+GGG 0.486261 4_1:+53379467 Msa0555360:three_prime_UTR
GCACTTCATTGAACACTAAG+AGG 0.487095 4_1:+53377602 Msa0555360:CDS
ATGATGGTGAAGGTGGTTCA+TGG 0.487129 4_1:-53379858 None:intergenic
AATTGGGTCCCCTGCAAACC+AGG 0.492034 4_1:-53379700 None:intergenic
GGCTATAATGCAAGCTAGGA+TGG 0.493126 4_1:+53379201 Msa0555360:CDS
TTGAAAATCACTTGAAAGGA+GGG 0.498570 4_1:-53379624 None:intergenic
AATTAATTCTTGAACACCAA+TGG 0.503254 4_1:+53379719 Msa0555360:three_prime_UTR
TTCATTGAACACTAAGAGGC+TGG 0.503842 4_1:+53377606 Msa0555360:CDS
TGCCACGCAGTTTAGCTCCA+AGG 0.505877 4_1:-53379431 None:intergenic
AACAAAGAGATAAATATGTC+AGG 0.505887 4_1:+53377119 Msa0555360:exon
CCAGCAGATAATTAGCAAGT+TGG 0.509376 4_1:+53379401 Msa0555360:CDS
ATAGCCTTCTTGAGTTCAGT+TGG 0.514235 4_1:-53379184 None:intergenic
AGAAGGCTATAATGCAAGCT+AGG 0.515044 4_1:+53379197 Msa0555360:CDS
TTCATTGGCCTGGTTTGCAG+GGG 0.520676 4_1:+53379692 Msa0555360:three_prime_UTR
GAGGAGCGAACGTTTCTTGA+CGG 0.526730 4_1:-53377264 None:intergenic
CGGCGTTGGGAGCTTTCTTG+CGG 0.528475 4_1:-53377181 None:intergenic
GCCACGCAGTTTAGCTCCAA+GGG 0.531362 4_1:-53379430 None:intergenic
GGCTTTCTCATCTTTCTTGG+CGG 0.532271 4_1:-53377204 None:intergenic
ACTTAGGATTCCAAGTCCAT+TGG 0.537779 4_1:-53379735 None:intergenic
CTTGAAAATCACTTGAAAGG+AGG 0.538825 4_1:-53379625 None:intergenic
TACCTTGAAAATCACTTGAA+AGG 0.542425 4_1:-53379628 None:intergenic
CCTTCTAACAGCCACAATGA+TGG 0.543848 4_1:-53379874 None:intergenic
GAACACTAAGAGGCTGGATG+AGG 0.556069 4_1:+53377612 Msa0555360:CDS
GACGGTATCGATATCGGCGC+CGG 0.571328 4_1:-53377246 None:intergenic
TAGGGTTCGTAGCTCAGTGA+AGG 0.583142 4_1:-53377068 None:intergenic
TTTCTCATCTTTCTTGGCGG+CGG 0.585231 4_1:-53377201 None:intergenic
TGTACCAACTGAACTCAAGA+AGG 0.594355 4_1:+53379180 Msa0555360:CDS
TCGTACTCTTGGATCACTTG+AGG 0.598699 4_1:-53379357 None:intergenic
AGCCACAATGATGGTGAAGG+TGG 0.614245 4_1:-53379865 None:intergenic
TGATCCAAGAGTACGAGTCA+GGG 0.620671 4_1:+53379364 Msa0555360:CDS
TATCGATATCGGCGCCGGCA+CGG 0.624694 4_1:-53377241 None:intergenic
AACAGCCACAATGATGGTGA+AGG 0.631667 4_1:-53379868 None:intergenic
CGAACAACAGGTGAAAATCA+CGG 0.647680 4_1:-53379572 None:intergenic
GCCCTTGGAGCTAAACTGCG+TGG 0.653713 4_1:+53379429 Msa0555360:CDS
AACCACCTTCACCATCATTG+TGG 0.664900 4_1:+53379863 Msa0555360:three_prime_UTR
TCATTGTGGCTGTTAGAAGG+TGG 0.774728 4_1:+53379877 Msa0555360:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTAATTAATTAGTTTT+GGG - chr4_1:53377292-53377311 None:intergenic 10.0%
!!! AAACATTAATTAATTAGTTT+TGG - chr4_1:53377293-53377312 None:intergenic 10.0%
!!! AAATTTCAATATATTGATTA+TGG + chr4_1:53377483-53377502 Msa0555360:intron 10.0%
!!! TTTTTTTTTTTTTGTGATTT+TGG + chr4_1:53377512-53377531 Msa0555360:intron 10.0%
!!! TAATGTTTTTTTTTAACTCT+TGG + chr4_1:53377305-53377324 Msa0555360:intron 15.0%
!!! TTCGTAGATAAAAATAATTT+TGG + chr4_1:53377418-53377437 Msa0555360:intron 15.0%
!! TTCAAATTATATAATCTGAT+GGG - chr4_1:53378647-53378666 None:intergenic 15.0%
!! TTTCAAATTATATAATCTGA+TGG - chr4_1:53378648-53378667 None:intergenic 15.0%
!! GTATATGTAAAACATATAAA+TGG + chr4_1:53379542-53379561 Msa0555360:three_prime_UTR 15.0%
!!! TTTGATGATTATTCAGTATT+AGG + chr4_1:53377442-53377461 Msa0555360:intron 20.0%
!!! TTGATGATTATTCAGTATTA+GGG + chr4_1:53377443-53377462 Msa0555360:intron 20.0%
!!! TTTTTTTTTGTGATTTTGGA+TGG + chr4_1:53377516-53377535 Msa0555360:intron 20.0%
!!! TTTTTGAATGAGTAGTTATA+TGG + chr4_1:53377746-53377765 Msa0555360:intron 20.0%
!!! TTTTGAATGAGTAGTTATAT+GGG + chr4_1:53377747-53377766 Msa0555360:intron 20.0%
!!! TCATATAGAAAAATTTTGTG+TGG + chr4_1:53377823-53377842 Msa0555360:intron 20.0%
!!! ATTTTGAATTCATGATGTTT+TGG + chr4_1:53378006-53378025 Msa0555360:intron 20.0%
!! CAGAAAAGAAGAAAAAAAAA+TGG - chr4_1:53378227-53378246 None:intergenic 20.0%
!! AGGAGAAATAAAAAAATAAC+TGG + chr4_1:53378371-53378390 Msa0555360:intron 20.0%
!! GGAGAAATAAAAAAATAACT+GGG + chr4_1:53378372-53378391 Msa0555360:intron 20.0%
!! TCAAATTATATAATCTGATG+GGG - chr4_1:53378646-53378665 None:intergenic 20.0%
!! AACCCAATACATATAAATTA+CGG - chr4_1:53378830-53378849 None:intergenic 20.0%
!! CATTTCCCTAAAAAATATAA+TGG - chr4_1:53379084-53379103 None:intergenic 20.0%
!! CATGTTATCAATAACAAAAA+TGG + chr4_1:53379309-53379328 Msa0555360:intron 20.0%
!! AAGTTACAAATTTCAAACTT+AGG - chr4_1:53379754-53379773 None:intergenic 20.0%
! AACAAAGAGATAAATATGTC+AGG + chr4_1:53377119-53377138 Msa0555360:exon 25.0%
!!! AATTAATTAGTTTTGGGAAG+AGG - chr4_1:53377286-53377305 None:intergenic 25.0%
! TTTGAATGAGTAGTTATATG+GGG + chr4_1:53377748-53377767 Msa0555360:intron 25.0%
! ATATGATTCAAACTCAATCA+CGG + chr4_1:53378197-53378216 Msa0555360:intron 25.0%
! TAGATTTCGAAATCAAATTG+TGG + chr4_1:53378295-53378314 Msa0555360:intron 25.0%
! AGATTTCGAAATCAAATTGT+GGG + chr4_1:53378296-53378315 Msa0555360:intron 25.0%
! ATTAAGTAAATGCTAAACAG+TGG - chr4_1:53378440-53378459 None:intergenic 25.0%
! AGCATTTACTTAATTGTTTG+TGG + chr4_1:53378446-53378465 Msa0555360:intron 25.0%
! GCATTTACTTAATTGTTTGT+GGG + chr4_1:53378447-53378466 Msa0555360:intron 25.0%
! CATTTACTTAATTGTTTGTG+GGG + chr4_1:53378448-53378467 Msa0555360:intron 25.0%
! TCTCCGTAATTTATATGTAT+TGG + chr4_1:53378824-53378843 Msa0555360:intron 25.0%
! CTCCGTAATTTATATGTATT+GGG + chr4_1:53378825-53378844 Msa0555360:intron 25.0%
!! TCATGATCAAAATTTTCTCT+TGG + chr4_1:53378913-53378932 Msa0555360:intron 25.0%
! ATATCATGCTTAATAGATAG+AGG + chr4_1:53379047-53379066 Msa0555360:intron 25.0%
!!! TGTCCACCATTATATTTTTT+AGG + chr4_1:53379075-53379094 Msa0555360:intron 25.0%
!!! GTCCACCATTATATTTTTTA+GGG + chr4_1:53379076-53379095 Msa0555360:intron 25.0%
! TTCCCTAAAAAATATAATGG+TGG - chr4_1:53379081-53379100 None:intergenic 25.0%
!! TTGGTGTTCAAGAATTAATT+GGG - chr4_1:53379719-53379738 None:intergenic 25.0%
!! ATTGGTGTTCAAGAATTAAT+TGG - chr4_1:53379720-53379739 None:intergenic 25.0%
! AATTAATTCTTGAACACCAA+TGG + chr4_1:53379719-53379738 Msa0555360:three_prime_UTR 25.0%
! TGTTTTTCTGTGTCTGTTTA+GGG - chr4_1:53377089-53377108 None:intergenic 30.0%
TATGAAAATACTTTAGCTGC+AGG - chr4_1:53377808-53377827 None:intergenic 30.0%
! AAAATTTTGTGTGGAACATG+TGG + chr4_1:53377832-53377851 Msa0555360:intron 30.0%
TTCTACAGCATAAATTTGAG+CGG - chr4_1:53378060-53378079 None:intergenic 30.0%
!!! AACATATTTTGAGTTGTGCT+TGG - chr4_1:53378102-53378121 None:intergenic 30.0%
CAAAATATGTTTGCATAGCT+TGG + chr4_1:53378111-53378130 Msa0555360:intron 30.0%
CAGAAAACAGGACTAAAATA+TGG + chr4_1:53378336-53378355 Msa0555360:intron 30.0%
! AATTATAGGGTGGATTTGAA+TGG + chr4_1:53378472-53378491 Msa0555360:intron 30.0%
! AAATTTTCTCTTGGCTTCAT+TGG + chr4_1:53378922-53378941 Msa0555360:intron 30.0%
TAAAGAACATAAAGAGACTG+AGG - chr4_1:53379279-53379298 None:intergenic 30.0%
TTGAAAATCACTTGAAAGGA+GGG - chr4_1:53379627-53379646 None:intergenic 30.0%
TACCTTGAAAATCACTTGAA+AGG - chr4_1:53379631-53379650 None:intergenic 30.0%
TTGTGTTTATCTTGGTTCAT+TGG + chr4_1:53379677-53379696 Msa0555360:three_prime_UTR 30.0%
!!! AACCTTGTTTTTTCGACAAA+TGG - chr4_1:53379831-53379850 None:intergenic 30.0%
! GTGTTTTTCTGTGTCTGTTT+AGG - chr4_1:53377090-53377109 None:intergenic 35.0%
GAGATAAATATGTCAGGTCT+AGG + chr4_1:53377125-53377144 Msa0555360:exon 35.0%
TAGGCCATATTTCTCAAGAT+TGG + chr4_1:53377144-53377163 Msa0555360:CDS 35.0%
AGGCCATATTTCTCAAGATT+GGG + chr4_1:53377145-53377164 Msa0555360:CDS 35.0%
GTTCCCAATCTTGAGAAATA+TGG - chr4_1:53377151-53377170 None:intergenic 35.0%
!! TTTCTTGACGGTATCGATAT+CGG - chr4_1:53377255-53377274 None:intergenic 35.0%
! TGATTTTGGATGGTGATTGT+TGG + chr4_1:53377526-53377545 Msa0555360:intron 35.0%
CCATTGATAGAATAGAACCT+CGG + chr4_1:53377717-53377736 Msa0555360:intron 35.0%
!! CTTTAAGCACTGCTAACTTT+TGG + chr4_1:53377946-53377965 Msa0555360:intron 35.0%
GCTTGGACTAAAAATCAACA+TGG - chr4_1:53378085-53378104 None:intergenic 35.0%
! AAATATGGCTTGTACACTCA+AGG + chr4_1:53378351-53378370 Msa0555360:intron 35.0%
ATTGTTTGTGGGGCAATTAT+AGG + chr4_1:53378458-53378477 Msa0555360:intron 35.0%
TTGTTTGTGGGGCAATTATA+GGG + chr4_1:53378459-53378478 Msa0555360:intron 35.0%
! CATTGAAACTGTTTTCGCAA+AGG + chr4_1:53378526-53378545 Msa0555360:intron 35.0%
TGTATTGGAAGTCCATTTAG+CGG + chr4_1:53378569-53378588 Msa0555360:intron 35.0%
ACTGTCCTTGTAAAGTTGAA+TGG + chr4_1:53378760-53378779 Msa0555360:intron 35.0%
GCTAACCATTCAACTTTACA+AGG - chr4_1:53378768-53378787 None:intergenic 35.0%
AGTGACAACAAAGACGTTAA+AGG - chr4_1:53378798-53378817 None:intergenic 35.0%
ATCTCATCAAGCATGAGTTA+AGG - chr4_1:53378858-53378877 None:intergenic 35.0%
AATACAGCAACTGAACAATC+AGG - chr4_1:53378965-53378984 None:intergenic 35.0%
! AGGGAAATGTTAACCACTTT+TGG + chr4_1:53379095-53379114 Msa0555360:intron 35.0%
AACATAAAGAGACTGAGGTT+GGG - chr4_1:53379274-53379293 None:intergenic 35.0%
!! AATTATCTGCTGGTTTGGAA+TGG - chr4_1:53379394-53379413 None:intergenic 35.0%
! TTGCTAATTATCTGCTGGTT+TGG - chr4_1:53379399-53379418 None:intergenic 35.0%
ATTTCGCTTATTCGAACAAC+AGG - chr4_1:53379587-53379606 None:intergenic 35.0%
CTTGAAAATCACTTGAAAGG+AGG - chr4_1:53379628-53379647 None:intergenic 35.0%
!! CTCCTTTCAAGTGATTTTCA+AGG + chr4_1:53379626-53379645 Msa0555360:three_prime_UTR 35.0%
! AGTGAGTGTTGTGTTTATCT+TGG + chr4_1:53379669-53379688 Msa0555360:three_prime_UTR 35.0%
CTCCATTTGTCGAAAAAACA+AGG + chr4_1:53379826-53379845 Msa0555360:three_prime_UTR 35.0%
! TAGCTCAGTGAAGGTGTAAA+TGG - chr4_1:53377062-53377081 None:intergenic 40.0%
CGTATTGCGATTGTTGTAGT+TGG + chr4_1:53377335-53377354 Msa0555360:intron 40.0%
GCACTTCATTGAACACTAAG+AGG + chr4_1:53377602-53377621 Msa0555360:CDS 40.0%
TTCATTGAACACTAAGAGGC+TGG + chr4_1:53377606-53377625 Msa0555360:CDS 40.0%
CCGAGGTTCTATTCTATCAA+TGG - chr4_1:53377720-53377739 None:intergenic 40.0%
TCATTCAAAAATCGACACCG+AGG - chr4_1:53377737-53377756 None:intergenic 40.0%
GTTATATGGGGAGAATGATG+AGG + chr4_1:53377760-53377779 Msa0555360:intron 40.0%
TTTGTGTGGAACATGTGGTT+TGG + chr4_1:53377837-53377856 Msa0555360:intron 40.0%
ATGTTTGCATAGCTTGGACA+TGG + chr4_1:53378117-53378136 Msa0555360:intron 40.0%
TGTTTGCATAGCTTGGACAT+GGG + chr4_1:53378118-53378137 Msa0555360:intron 40.0%
TCAAATTGTGGGTCAAGTTC+CGG + chr4_1:53378307-53378326 Msa0555360:intron 40.0%
CAATGCATGAATCCGCTAAA+TGG - chr4_1:53378584-53378603 None:intergenic 40.0%
AGAGAGAGCGATCACATAAT+GGG - chr4_1:53378681-53378700 None:intergenic 40.0%
AAGAGAGAGCGATCACATAA+TGG - chr4_1:53378682-53378701 None:intergenic 40.0%
! TCTTGCTGTGTGTGTAATTG+TGG + chr4_1:53378702-53378721 Msa0555360:intron 40.0%
!! ACACAGCATGCTTTGGAATT+TGG + chr4_1:53379005-53379024 Msa0555360:intron 40.0%
ACAATCACATGACTCATGAC+TGG + chr4_1:53379136-53379155 Msa0555360:intron 40.0%
TGTACCAACTGAACTCAAGA+AGG + chr4_1:53379180-53379199 Msa0555360:CDS 40.0%
ATAGCCTTCTTGAGTTCAGT+TGG - chr4_1:53379187-53379206 None:intergenic 40.0%
AGAAGGCTATAATGCAAGCT+AGG + chr4_1:53379197-53379216 Msa0555360:CDS 40.0%
ACATAAAGAGACTGAGGTTG+GGG - chr4_1:53379273-53379292 None:intergenic 40.0%
GAACATAAAGAGACTGAGGT+TGG - chr4_1:53379275-53379294 None:intergenic 40.0%
!! CCAACTTGCTAATTATCTGC+TGG - chr4_1:53379404-53379423 None:intergenic 40.0%
CCAGCAGATAATTAGCAAGT+TGG + chr4_1:53379401-53379420 Msa0555360:CDS 40.0%
TGATTTCATGATTGTGCGCT+GGG + chr4_1:53379467-53379486 Msa0555360:three_prime_UTR 40.0%
CGAACAACAGGTGAAAATCA+CGG - chr4_1:53379575-53379594 None:intergenic 40.0%
TTTATCTTGGTTCATTGGCC+TGG + chr4_1:53379682-53379701 Msa0555360:three_prime_UTR 40.0%
! TTCTTGAACACCAATGGACT+TGG + chr4_1:53379725-53379744 Msa0555360:three_prime_UTR 40.0%
ACTTAGGATTCCAAGTCCAT+TGG - chr4_1:53379738-53379757 None:intergenic 40.0%
TGACAAGTACTCTGCAAATC+AGG - chr4_1:53379784-53379803 None:intergenic 40.0%
TTTCTCATCTTTCTTGGCGG+CGG - chr4_1:53377204-53377223 None:intergenic 45.0%
GGCTTTCTCATCTTTCTTGG+CGG - chr4_1:53377207-53377226 None:intergenic 45.0%
GACGGCTTTCTCATCTTTCT+TGG - chr4_1:53377210-53377229 None:intergenic 45.0%
!! TGTCTACTGACTCTTGCAAC+TGG + chr4_1:53377872-53377891 Msa0555360:intron 45.0%
TTGGTCCAATGTCCTCTAGT+AGG + chr4_1:53378150-53378169 Msa0555360:intron 45.0%
CGTTACCTACTAGAGGACAT+TGG - chr4_1:53378158-53378177 None:intergenic 45.0%
AGTCTGTCGTTACCTACTAG+AGG - chr4_1:53378165-53378184 None:intergenic 45.0%
TTCCGGATCATGCAGAAAAC+AGG + chr4_1:53378324-53378343 Msa0555360:intron 45.0%
! GTCCTGTTTTCTGCATGATC+CGG - chr4_1:53378329-53378348 None:intergenic 45.0%
TTTGTGGGGCAATTATAGGG+TGG + chr4_1:53378462-53378481 Msa0555360:intron 45.0%
! GTTTTCGCAAAGGAAAGCCT+TGG + chr4_1:53378536-53378555 Msa0555360:intron 45.0%
!! AAAGGAAAGCCTTGGCTCTA+AGG + chr4_1:53378544-53378563 Msa0555360:intron 45.0%
CAATACAAGCCTTAGAGCCA+AGG - chr4_1:53378556-53378575 None:intergenic 45.0%
!! CTTGGCTCTAAGGCTTGTAT+TGG + chr4_1:53378554-53378573 Msa0555360:intron 45.0%
GAGAGAGCGATCACATAATG+GGG - chr4_1:53378680-53378699 None:intergenic 45.0%
GATGTGCAGGAATCCAAAAG+TGG - chr4_1:53379111-53379130 None:intergenic 45.0%
GTGATTGTCAACAGATGTGC+AGG - chr4_1:53379124-53379143 None:intergenic 45.0%
GGCTATAATGCAAGCTAGGA+TGG + chr4_1:53379201-53379220 Msa0555360:CDS 45.0%
ATAAAGAGACTGAGGTTGGG+GGG - chr4_1:53379271-53379290 None:intergenic 45.0%
CATAAAGAGACTGAGGTTGG+GGG - chr4_1:53379272-53379291 None:intergenic 45.0%
TCGTACTCTTGGATCACTTG+AGG - chr4_1:53379360-53379379 None:intergenic 45.0%
! TGATCCAAGAGTACGAGTCA+GGG + chr4_1:53379364-53379383 Msa0555360:CDS 45.0%
CAGCGCACAATCATGAAATC+AGG - chr4_1:53379468-53379487 None:intergenic 45.0%
CTGATTTCATGATTGTGCGC+TGG + chr4_1:53379466-53379485 Msa0555360:three_prime_UTR 45.0%
GGGTTTGCATGTCACTCTTA+AGG + chr4_1:53379487-53379506 Msa0555360:three_prime_UTR 45.0%
! ATGATGGTGAAGGTGGTTCA+TGG - chr4_1:53379861-53379880 None:intergenic 45.0%
AACCACCTTCACCATCATTG+TGG + chr4_1:53379863-53379882 Msa0555360:three_prime_UTR 45.0%
! AACAGCCACAATGATGGTGA+AGG - chr4_1:53379871-53379890 None:intergenic 45.0%
CCTTCTAACAGCCACAATGA+TGG - chr4_1:53379877-53379896 None:intergenic 45.0%
CCATCATTGTGGCTGTTAGA+AGG + chr4_1:53379874-53379893 Msa0555360:three_prime_UTR 45.0%
TCATTGTGGCTGTTAGAAGG+TGG + chr4_1:53379877-53379896 Msa0555360:three_prime_UTR 45.0%
!!! TATTTTTTTTATATCTTAAT+TGG - chr4_1:53379964-53379983 None:intergenic 5.0%
TAGGGTTCGTAGCTCAGTGA+AGG - chr4_1:53377071-53377090 None:intergenic 50.0%
GAGGAGCGAACGTTTCTTGA+CGG - chr4_1:53377267-53377286 None:intergenic 50.0%
GAACACTAAGAGGCTGGATG+AGG + chr4_1:53377612-53377631 Msa0555360:CDS 50.0%
! TGGACATGGGAGTGTGATCT+TGG + chr4_1:53378131-53378150 Msa0555360:intron 50.0%
ACTGTGCACACAGCATGCTT+TGG + chr4_1:53378998-53379017 Msa0555360:intron 50.0%
! GTGATCCAAGAGTACGAGTC+AGG + chr4_1:53379363-53379382 Msa0555360:CDS 50.0%
! CTTTCCCTGACTCGTACTCT+TGG - chr4_1:53379371-53379390 None:intergenic 50.0%
! GTTCATTGGCCTGGTTTGCA+GGG + chr4_1:53379691-53379710 Msa0555360:three_prime_UTR 50.0%
! TTCATTGGCCTGGTTTGCAG+GGG + chr4_1:53379692-53379711 Msa0555360:three_prime_UTR 50.0%
! AGCCACAATGATGGTGAAGG+TGG - chr4_1:53379868-53379887 None:intergenic 50.0%
GCTTTCTTGCGGATGACGAC+TGG - chr4_1:53377173-53377192 None:intergenic 55.0%
! AGCAAGTTGGAGAGAGCCCT+TGG + chr4_1:53379414-53379433 Msa0555360:CDS 55.0%
! GCCACGCAGTTTAGCTCCAA+GGG - chr4_1:53379433-53379452 None:intergenic 55.0%
! TGCCACGCAGTTTAGCTCCA+AGG - chr4_1:53379434-53379453 None:intergenic 55.0%
! GGTTCATTGGCCTGGTTTGC+AGG + chr4_1:53379690-53379709 Msa0555360:three_prime_UTR 55.0%
AATTGGGTCCCCTGCAAACC+AGG - chr4_1:53379703-53379722 None:intergenic 55.0%
! CGGCGTTGGGAGCTTTCTTG+CGG - chr4_1:53377184-53377203 None:intergenic 60.0%
TCTTTCTTGGCGGCGGCGTT+GGG - chr4_1:53377197-53377216 None:intergenic 60.0%
ATCTTTCTTGGCGGCGGCGT+TGG - chr4_1:53377198-53377217 None:intergenic 60.0%
!! TATCGATATCGGCGCCGGCA+CGG - chr4_1:53377244-53377263 None:intergenic 60.0%
!! GACGGTATCGATATCGGCGC+CGG - chr4_1:53377249-53377268 None:intergenic 60.0%
GCCCTTGGAGCTAAACTGCG+TGG + chr4_1:53379429-53379448 Msa0555360:CDS 60.0%
! GGCACGGCGAGCGGCGTTGA+CGG - chr4_1:53377228-53377247 None:intergenic 75.0%
GTCAACGCCGCTCGCCGTGC+CGG + chr4_1:53377227-53377246 Msa0555360:CDS 75.0%
!!! ATCGGCGCCGGCACGGCGAG+CGG - chr4_1:53377237-53377256 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 53377050 53379996 53377050 ID=Msa0555360;Name=Msa0555360
chr4_1 mRNA 53377050 53379996 53377050 ID=Msa0555360-mRNA-1;Parent=Msa0555360;Name=Msa0555360-mRNA-1;_AED=0.04;_eAED=0.04;_QI=84|1|1|1|1|1|4|535|142
chr4_1 exon 53377050 53377275 53377050 ID=Msa0555360-mRNA-1:exon:15473;Parent=Msa0555360-mRNA-1
chr4_1 exon 53377569 53377652 53377569 ID=Msa0555360-mRNA-1:exon:15474;Parent=Msa0555360-mRNA-1
chr4_1 exon 53379173 53379255 53379173 ID=Msa0555360-mRNA-1:exon:15475;Parent=Msa0555360-mRNA-1
chr4_1 exon 53379342 53379996 53379342 ID=Msa0555360-mRNA-1:exon:15476;Parent=Msa0555360-mRNA-1
chr4_1 five_prime_UTR 53377050 53377133 53377050 ID=Msa0555360-mRNA-1:five_prime_utr;Parent=Msa0555360-mRNA-1
chr4_1 CDS 53377134 53377275 53377134 ID=Msa0555360-mRNA-1:cds;Parent=Msa0555360-mRNA-1
chr4_1 CDS 53377569 53377652 53377569 ID=Msa0555360-mRNA-1:cds;Parent=Msa0555360-mRNA-1
chr4_1 CDS 53379173 53379255 53379173 ID=Msa0555360-mRNA-1:cds;Parent=Msa0555360-mRNA-1
chr4_1 CDS 53379342 53379461 53379342 ID=Msa0555360-mRNA-1:cds;Parent=Msa0555360-mRNA-1
chr4_1 three_prime_UTR 53379462 53379996 53379462 ID=Msa0555360-mRNA-1:three_prime_utr;Parent=Msa0555360-mRNA-1
Gene Sequence

>Msa0555360

ATGTCAGGTCTAGGCCATATTTCTCAAGATTGGGAACCAGTCGTCATCCGCAAGAAAGCTCCCAACGCCGCCGCCAAGAAAGATGAGAAAGCCGTCAACGCCGCTCGCCGTGCCGGCGCCGATATCGATACCGTCAAGAAACATAATGCTGCAACAAACAAAGCTGCATCTAGCAGCACTTCATTGAACACTAAGAGGCTGGATGAGGATACTGAGAATCTAGCTCATGATCGTGTACCAACTGAACTCAAGAAGGCTATAATGCAAGCTAGGATGGACAAAAAGCTTACTCAGTCTCAGCTTGCTCAAATCATCAATGAGAAGCCTCAAGTGATCCAAGAGTACGAGTCAGGGAAAGCCATTCCAAACCAGCAGATAATTAGCAAGTTGGAGAGAGCCCTTGGAGCTAAACTGCGTGGCAAGAAATGA
Protein sequence

>Msa0555360

MSGLGHISQDWEPVVIRKKAPNAAAKKDEKAVNAARRAGADIDTVKKHNAATNKAASSSTSLNTKRLDEDTENLAHDRVPTELKKAIMQARMDKKLTQSQLAQIINEKPQVIQEYESGKAIPNQQIISKLERALGAKLRGKK*