Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0559590 | XP_003608360.1 | 38.197 | 233 | 99 | 8 | 347 | 540 | 31 | 257 | 9.21e-22 | 113 |
| Msa0559590 | XP_003608360.1 | 59.848 | 132 | 45 | 2 | 537 | 664 | 321 | 448 | 1.51e-30 | 140 |
| Msa0559590 | XP_003608360.1 | 74.930 | 355 | 35 | 5 | 1 | 301 | 1 | 355 | 1.05e-165 | 503 |
| Msa0559590 | XP_003608360.1 | 97.917 | 48 | 1 | 0 | 294 | 341 | 620 | 667 | 2.62e-18 | 102 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0559590 | sp|Q9LSL5|LRK92_ARATH | 33.166 | 398 | 149 | 15 | 369 | 664 | 55 | 437 | 8.33e-40 | 159 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0559590 | G7JU23 | 38.197 | 233 | 99 | 8 | 347 | 540 | 31 | 257 | 4.40e-22 | 113 |
| Msa0559590 | G7JU23 | 59.848 | 132 | 45 | 2 | 537 | 664 | 321 | 448 | 7.21e-31 | 140 |
| Msa0559590 | G7JU23 | 74.930 | 355 | 35 | 5 | 1 | 301 | 1 | 355 | 4.99e-166 | 503 |
| Msa0559590 | G7JU23 | 97.917 | 48 | 1 | 0 | 294 | 341 | 620 | 667 | 1.25e-18 | 102 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0559590 | MtrunA17_Chr4g0049551 | 74.930 | 355 | 35 | 5 | 1 | 301 | 1 | 355 | 9.61e-170 | 503 |
| Msa0559590 | MtrunA17_Chr4g0049551 | 59.848 | 132 | 45 | 2 | 537 | 664 | 321 | 448 | 1.39e-34 | 140 |
| Msa0559590 | MtrunA17_Chr4g0049551 | 38.197 | 233 | 99 | 8 | 347 | 540 | 31 | 257 | 8.47e-26 | 113 |
| Msa0559590 | MtrunA17_Chr4g0049551 | 97.917 | 48 | 1 | 0 | 294 | 341 | 620 | 667 | 2.41e-22 | 102 |
| Msa0559590 | MtrunA17_Chr4g0049551 | 52.459 | 61 | 29 | 0 | 662 | 722 | 604 | 664 | 8.20e-13 | 72.0 |
| Msa0559590 | MtrunA17_Chr4g0049561 | 64.286 | 420 | 56 | 6 | 342 | 667 | 19 | 438 | 3.40e-159 | 475 |
| Msa0559590 | MtrunA17_Chr4g0049561 | 34.604 | 341 | 167 | 12 | 14 | 301 | 5 | 342 | 2.73e-35 | 142 |
| Msa0559590 | MtrunA17_Chr4g0049561 | 92.188 | 64 | 5 | 0 | 663 | 726 | 592 | 655 | 1.02e-29 | 125 |
| Msa0559590 | MtrunA17_Chr7g0274651 | 42.898 | 352 | 141 | 7 | 10 | 301 | 13 | 364 | 1.11e-76 | 260 |
| Msa0559590 | MtrunA17_Chr7g0274651 | 62.205 | 127 | 44 | 1 | 542 | 664 | 331 | 457 | 1.32e-43 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0559590 | AT5G65600.1 | 33.166 | 398 | 149 | 15 | 369 | 664 | 55 | 437 | 8.47e-41 | 159 |
| Msa0559590 | AT5G10530.1 | 33.929 | 336 | 129 | 11 | 414 | 664 | 95 | 422 | 3.15e-40 | 157 |
| Msa0559590 | AT5G10530.1 | 26.689 | 296 | 165 | 14 | 45 | 294 | 33 | 322 | 6.27e-13 | 72.8 |
| Msa0559590 | AT3G55550.1 | 50.000 | 116 | 54 | 1 | 553 | 664 | 318 | 433 | 2.22e-31 | 131 |
| Msa0559590 | AT5G06740.1 | 34.494 | 316 | 135 | 10 | 417 | 665 | 106 | 416 | 3.84e-31 | 130 |
| Msa0559590 | AT5G06740.2 | 34.494 | 316 | 135 | 10 | 417 | 665 | 118 | 428 | 4.05e-31 | 130 |
| Msa0559590 | AT3G59730.1 | 49.123 | 114 | 54 | 1 | 554 | 663 | 308 | 421 | 2.63e-27 | 117 |
| Msa0559590 | AT3G59700.1 | 49.123 | 114 | 54 | 1 | 554 | 663 | 311 | 424 | 7.08e-27 | 117 |
| Msa0559590 | AT3G59750.1 | 48.246 | 114 | 55 | 1 | 554 | 663 | 276 | 389 | 1.49e-26 | 115 |
| Msa0559590 | AT3G59740.1 | 49.550 | 111 | 52 | 1 | 554 | 660 | 306 | 416 | 2.24e-26 | 115 |
Find 157 sgRNAs with CRISPR-Local
Find 415 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAACAATTACAGTGTC+GGG | 0.492245 | 4_1:+58780728 | Msa0559590:CDS |
| AAAAGGGGTCTGGACCTAAG+AGG | 0.545718 | 4_1:+58781544 | Msa0559590:CDS |
| AAACAAGATGGGATTGAAGG+TGG | 0.597183 | 4_1:+58781401 | Msa0559590:CDS |
| AAACACCGCCAAAACCACCT+TGG | 0.613588 | 4_1:-58784894 | None:intergenic |
| AAAGAGCCACCAGAGCTTGA+TGG | 0.425435 | 4_1:+58783832 | Msa0559590:CDS |
| AAAGGATATCAGCAGATTCA+AGG | 0.486374 | 4_1:+58784959 | Msa0559590:CDS |
| AAAGTTAATCATATAGGAGG+TGG | 0.519984 | 4_1:+58784087 | Msa0559590:CDS |
| AACAACAAAGGAAAACTTGG+TGG | 0.578066 | 4_1:-58780815 | None:intergenic |
| AACAAGATGGGATTGAAGGT+GGG | 0.552389 | 4_1:+58781402 | Msa0559590:CDS |
| AACTACTTCTGATCAGGATA+TGG | 0.360219 | 4_1:+58784777 | Msa0559590:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACATCACCATTCTTAAAGTA+GGG | - | chr4_1:58780643-58780662 | None:intergenic | 30.0% | |
| CAAAACAACAATTACAGTGT+CGG | + | chr4_1:58780727-58780746 | Msa0559590:CDS | 30.0% | |
| AAAACAACAATTACAGTGTC+GGG | + | chr4_1:58780728-58780747 | Msa0559590:CDS | 30.0% | |
| TTGAAGAATATTTCACAAGG+AGG | + | chr4_1:58780904-58780923 | Msa0559590:CDS | 30.0% | |
| TGTTGAATATTTGTCAACCA+CGG | - | chr4_1:58781090-58781109 | None:intergenic | 30.0% | |
| GGTTGACAAATATTCAACAA+TGG | + | chr4_1:58781091-58781110 | Msa0559590:CDS | 30.0% | |
| TATGATATGTACTCTTCAAC+TGG | - | chr4_1:58781201-58781220 | None:intergenic | 30.0% | |
| TTGAACTAAATGACCATGAT+CGG | - | chr4_1:58781311-58781330 | None:intergenic | 30.0% | |
| ATGGATGATGTATTCCAAAA+GGG | + | chr4_1:58781528-58781547 | Msa0559590:CDS | 30.0% | |
| AGAAACTTAGTACAACTTCT+TGG | + | chr4_1:58781768-58781787 | Msa0559590:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 58780526 | 58785797 | 58780526 | ID=Msa0559590;Name=Msa0559590 |
| chr4_1 | mRNA | 58780526 | 58785797 | 58780526 | ID=Msa0559590-mRNA-1;Parent=Msa0559590;Name=Msa0559590-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|0.3|0.18|11|0|726 |
| chr4_1 | exon | 58780526 | 58780753 | 58780526 | ID=Msa0559590-mRNA-1:exon:18030;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58780772 | 58780992 | 58780772 | ID=Msa0559590-mRNA-1:exon:18031;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58781071 | 58781296 | 58781071 | ID=Msa0559590-mRNA-1:exon:18032;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58781360 | 58781565 | 58781360 | ID=Msa0559590-mRNA-1:exon:18033;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58782382 | 58782524 | 58782382 | ID=Msa0559590-mRNA-1:exon:18034;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58783773 | 58783989 | 58783773 | ID=Msa0559590-mRNA-1:exon:18035;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58784038 | 58784250 | 58784038 | ID=Msa0559590-mRNA-1:exon:18036;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58784311 | 58784467 | 58784311 | ID=Msa0559590-mRNA-1:exon:18037;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58784742 | 58784922 | 58784742 | ID=Msa0559590-mRNA-1:exon:18038;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58784935 | 58785135 | 58784935 | ID=Msa0559590-mRNA-1:exon:18039;Parent=Msa0559590-mRNA-1 |
| chr4_1 | exon | 58785610 | 58785797 | 58785610 | ID=Msa0559590-mRNA-1:exon:18040;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58780526 | 58780753 | 58780526 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58780772 | 58780992 | 58780772 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58781071 | 58781296 | 58781071 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58781360 | 58781565 | 58781360 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58782382 | 58782524 | 58782382 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58783773 | 58783989 | 58783773 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58784038 | 58784250 | 58784038 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58784311 | 58784467 | 58784311 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58784742 | 58784922 | 58784742 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58784935 | 58785135 | 58784935 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| chr4_1 | CDS | 58785610 | 58785797 | 58785610 | ID=Msa0559590-mRNA-1:cds;Parent=Msa0559590-mRNA-1 |
| Gene Sequence |
>Msa0559590 ATGGCACCTTTGCATACTAGTCTTGACACTCAAACTCTTGTTTTACGTTATGCAATAGGAACAATTTCCACCCTCCAAATGATCCATCTTGCAAACTCACTATCATTTGATTACCCCTACTTTAAGAATGGTGATGTCAACTGGGAAGGGGATGCTTCTCCTTATAAAGGAGCTATTCAAATCTCTTCTAATACCCTTGACCAAAACAACAATTACAGTGTCGGGCGAATGCATCTCTGGGATTTGAACTCTGGAAACCTCTCTGACTTCACCACCAAGTTTTCCTTTGTTGTTTATTCAGACAAAAGAGATTATGGAGATGGAATGGTATTCTTCTTGGCTGATCCAGATCTCCCACTTTTGAAGAATATTTCACAAGGAGGTGGTCTTGGTCTTGTAGATGGAGATCAAGTGCTAAAATCAACTCAACACTCTTTTGTAGCAGTGGAGTCTAACATAACTAAGCCGTGGTTGACAAATATTCAACAATGGAGAGTTTACAACTGCAGCATTGAGTACAAATCAAGCACTCTAAATTTGAGTGTTTCATTCACCATGTATGATGATGATGATAAACCAGTTGAAGAGTACATATCATACAAAGTTGATTTGAGAGATTTCTTACCAGAGAGGGTTATAGTTGGCTTCTCTGCTGCAACAGGAAGATTGTATGAGGTAGTGGCACCAATTGCCAGTCCTACAATTAATGAAAAAGAAAACAAGATGGGATTGAAGGTGGGGCTTGGAATTGGAACAGGCTTGGCTGTGAGTTTATCAGGGCTGCTTTGTACTCTCTTGTGGAAGAGGAGTAGAGAAAGAAAAGAGTCAGGTTTTGATCTCAACATGGATGATGTATTCCAAAAGGGGTCTGGACCTAAGAGGCCTTCTATAAGACAAGTGATTCAGGTTCTCAAATTTGAGTCTCCTTCACCAATACTTCCAGAAAAAATGCCTGTGCCAACCTATCTTCCTCCAACAATAAAAGCACTTTTTTCTTCAATTTCGTCTATATATTGGACAAATATCCCTCTTGCACATGCTCACCTTGTGTCTTTTGATTACCCTATGTTTAGTTACGATTGTAAAGAGCCACCAGAGCTTGATGGAGATGCCACCATTGTAGATTCTGACAAAGTTATCCGACTCACAGGGTACCCTGATGACCCAAATAAGGCTTCTGGTGTTGGTCGAGTCACAAGTCCCAAACTTATCAAACTTTATGAGAATAGCTCAAATCAAGGTTCTTATGGAGATGGTTTGGCATTCTTCCTGGCAAGCTCAAATCTCCCAAAAGTTAATCATATAGGAGGTGGAGGGGGTTTTGGTCTTGTGCCTGAAAGCAAAGTTGCTTTAAACTCAACTGAGTATTCGTTTGTGTCAGTGGAGTTTGACACGTACCAAAATTCTTGGGACCCAAGATTCAACCATGTAGGAGTGAATGTCAATTCTGTGGTCATTGAGTACAGTTCAAGCAATAACTTTCTGACTGTTTCTTTCGCTGGATACAGATTGAATGCATGGCAAGAGCCGCAAAACTTTTCACACATCATTGATCTAAGAGAATATTTAAAAGACTATGTTATTGTTGGCATATCAGCTTCAACAGGAAAAAAGTATAAAGGAAAAGAAGAAGAACCTTCTTCAGAAACTACTTCTGATCAGGATATGGATGATGAATTTCGAATGAATGCTGGACCTAAGAAGATTAGTTATTATGAATTGTTGAATGCAACAAACAACTTTGAAGAGACACAAAAGCTTGGCCAAGGTGGTTTTGGCGGTGTTTATAAGGACTCAAACTCAGTTGCAGCTATAAAAAGGATATCAGCAGATTCAAGGCAGGGTATAAAGCAATACTCAGCAGAAGTGAAGATCATTAGCCAACTGAGGCATAGAAATTTGGTGAAACTCAATGGTTGGTGCCACAAGAAGAATGAACTCATCCTAATATACGAATACATGCCTAATGGCAGCTTAGACTTTCATCTTTTTCTTGTTGGACTTTGGTGTGCTAATCCAGATAACACTTCAAGACCATCTATAAAGAAAGTGATTAAGGTGCTCAATTTTGAAGCTCCATTACCAATTCTTCCACAAAACATGCCATTCTCAGCCTCTCTGTCTCCTACAACTAATGAGCAATTTTTCTCCGTTCCTTCTTTCTTTAGGTCTACAGGTTAG |
| Protein sequence |
>Msa0559590 MAPLHTSLDTQTLVLRYAIGTISTLQMIHLANSLSFDYPYFKNGDVNWEGDASPYKGAIQISSNTLDQNNNYSVGRMHLWDLNSGNLSDFTTKFSFVVYSDKRDYGDGMVFFLADPDLPLLKNISQGGGLGLVDGDQVLKSTQHSFVAVESNITKPWLTNIQQWRVYNCSIEYKSSTLNLSVSFTMYDDDDKPVEEYISYKVDLRDFLPERVIVGFSAATGRLYEVVAPIASPTINEKENKMGLKVGLGIGTGLAVSLSGLLCTLLWKRSRERKESGFDLNMDDVFQKGSGPKRPSIRQVIQVLKFESPSPILPEKMPVPTYLPPTIKALFSSISSIYWTNIPLAHAHLVSFDYPMFSYDCKEPPELDGDATIVDSDKVIRLTGYPDDPNKASGVGRVTSPKLIKLYENSSNQGSYGDGLAFFLASSNLPKVNHIGGGGGFGLVPESKVALNSTEYSFVSVEFDTYQNSWDPRFNHVGVNVNSVVIEYSSSNNFLTVSFAGYRLNAWQEPQNFSHIIDLREYLKDYVIVGISASTGKKYKGKEEEPSSETTSDQDMDDEFRMNAGPKKISYYELLNATNNFEETQKLGQGGFGGVYKDSNSVAAIKRISADSRQGIKQYSAEVKIISQLRHRNLVKLNGWCHKKNELILIYEYMPNGSLDFHLFLVGLWCANPDNTSRPSIKKVIKVLNFEAPLPILPQNMPFSASLSPTTNEQFFSVPSFFRSTG* |
