AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0562200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562200 MtrunA17_Chr8g0375251 96.465 679 24 0 1 679 93 771 0.0 1362
Msa0562200 MtrunA17_Chr4g0063461 57.851 121 34 1 25 145 7 110 9.93e-43 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562200 AT2G02160.1 40.191 627 282 28 3 596 5 571 8.65e-106 336

Find 218 sgRNAs with CRISPR-Local

Find 308 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAGTCCTTCTTTCTATT+TGG 0.122370 4_1:-61836106 None:intergenic
ATTTGGGCTGATCCCTTTCT+AGG 0.173898 4_1:-61836089 None:intergenic
GAGAGAGCCGCCACAGAAAT+TGG 0.190492 4_1:-61833797 None:intergenic
GATCCATCGATCTCTTCTTC+TGG 0.192710 4_1:+61833860 Msa0562200:five_prime_UTR
GAATGTGATGATGGGCTTTA+AGG 0.209581 4_1:-61833703 None:intergenic
AGAATTTCACTAAGTGGTTT+TGG 0.245906 4_1:-61836680 None:intergenic
TATCATGGGGAAGATGAATT+TGG 0.267482 4_1:+61835903 Msa0562200:CDS
GCTTGTTTACTACCATTATA+AGG 0.280011 4_1:-61835369 None:intergenic
TTTCTCAGAGAGTCGTCTCC+TGG 0.281678 4_1:+61835837 Msa0562200:CDS
CCTCTTCTCAGATTGAGATT+TGG 0.282214 4_1:+61833838 Msa0562200:five_prime_UTR
AGTCTTGCTGAACTTAAGTA+TGG 0.284200 4_1:+61836563 Msa0562200:CDS
TATTAACTCTCTAACGTTAC+AGG 0.288251 4_1:-61837305 None:intergenic
ATGAGTCCTTCTTTCTATTT+GGG 0.297001 4_1:-61836105 None:intergenic
TTCTCCCTTGACATGCAAAA+AGG 0.299512 4_1:+61834002 Msa0562200:CDS
TCCTCTTTCCAAATGATTGT+TGG 0.310747 4_1:-61836607 None:intergenic
ATCCATCGATCTCTTCTTCT+GGG 0.318828 4_1:+61833861 Msa0562200:five_prime_UTR
TTGGAAAGAGGAAAAGCTTC+AGG 0.319306 4_1:+61836618 Msa0562200:CDS
TTGACGGATCTCTCATTTGC+AGG 0.321400 4_1:-61836275 None:intergenic
ATCGATCTCTTCTTCTGGGT+TGG 0.324812 4_1:+61833865 Msa0562200:five_prime_UTR
CAGCCTCTTGACGGGTTGTT+AGG 0.337201 4_1:+61835264 Msa0562200:intron
CAACACCTGCAACATTTGCT+TGG 0.342566 4_1:-61835590 None:intergenic
CCAAAAGGTTTCTTAAAACT+TGG 0.344005 4_1:-61835531 None:intergenic
TCGATCTCTTCTTCTGGGTT+GGG 0.344716 4_1:+61833866 Msa0562200:five_prime_UTR
AAGAACAACACCAATCTTCT+TGG 0.345542 4_1:-61837041 None:intergenic
ACCCATGCACCGCCTTATAA+TGG 0.352929 4_1:+61835357 Msa0562200:CDS
GGAAGAAGAGTCCAAGATTC+AGG 0.353435 4_1:+61836811 Msa0562200:CDS
CCGGCAACTGCTGCTGCTGC+TGG 0.354940 4_1:+61835294 Msa0562200:CDS
GCCGTTTCCCTTCCTGCGCT+TGG 0.358186 4_1:-61836183 None:intergenic
ATCAGTGGCCGGTTGTCATC+TGG 0.358804 4_1:+61836416 Msa0562200:CDS
TCGTATTCATTCAAAGAGTC+TGG 0.359110 4_1:-61835954 None:intergenic
AGAAATTCTGATTACTATCA+TGG 0.361394 4_1:+61835888 Msa0562200:CDS
AGAGTAAGAGAATGTGATGA+TGG 0.369394 4_1:-61833712 None:intergenic
TTAAGCATGTAGGCCTCAAC+TGG 0.379353 4_1:+61837163 Msa0562200:three_prime_UTR
ATCTCTCATTTGCAGGCAAC+TGG 0.384962 4_1:-61836268 None:intergenic
TTACTGCCATTGGTAGGGTT+TGG 0.386685 4_1:-61835459 None:intergenic
AATAATGACCAACAATCATT+TGG 0.389220 4_1:+61836599 Msa0562200:CDS
CGTCATTGTCCTTACCACTA+TGG 0.395917 4_1:-61835812 None:intergenic
ATCCTAGAGATTGTTGGTAT+TGG 0.401206 4_1:+61835066 Msa0562200:CDS
TTTCTAGGTTCCCAGCCATA+TGG 0.401770 4_1:-61836074 None:intergenic
ATCATATGAGTCATAACCTT+GGG 0.402504 4_1:-61836039 None:intergenic
TCCATCAGCAGCCTGAATCT+TGG 0.402576 4_1:-61836822 None:intergenic
CTATGGAAATTGTGATCATC+TGG 0.404436 4_1:-61835795 None:intergenic
AAGTTTCTCCTCTCGTCATC+TGG 0.405913 4_1:-61836494 None:intergenic
CGATGAAGCTGCCGAAGATC+AGG 0.408429 4_1:+61836898 Msa0562200:CDS
AAATCAAGAGCAAGAATATA+TGG 0.412298 4_1:+61836994 Msa0562200:CDS
GTACATCATCTTCATATTGT+TGG 0.423888 4_1:-61836646 None:intergenic
TAAAACAAGTAAGCGTGGTT+TGG 0.434567 4_1:-61833680 None:intergenic
GACTTAGACACAGGAAGAAC+CGG 0.436176 4_1:+61836359 Msa0562200:CDS
AGGATCCAGGATAGATTAAG+AGG 0.436261 4_1:+61836464 Msa0562200:CDS
ACTTCCCAAGCAAATGTTGC+AGG 0.436467 4_1:+61835585 Msa0562200:CDS
ACCTTCATACTCCTGATCTT+CGG 0.437886 4_1:-61836909 None:intergenic
GAAGATGATGTACCATTTGA+AGG 0.438650 4_1:+61836656 Msa0562200:CDS
CAAGTTAAAGAGACAATATA+AGG 0.443412 4_1:-61837125 None:intergenic
GAATCTGAAGTTGTGGCTGT+TGG 0.444668 4_1:-61833906 None:intergenic
CTTATATTGTCTCTTTAACT+TGG 0.445069 4_1:+61837126 Msa0562200:three_prime_UTR
ACCTTCCTCTTAATCTATCC+TGG 0.445108 4_1:-61836469 None:intergenic
AACACCTGCAACATTTGCTT+GGG 0.445165 4_1:-61835589 None:intergenic
TCCTTCAGAATTTCACTAAG+TGG 0.448374 4_1:-61836686 None:intergenic
AGGTGACTGTCACCACCATT+TGG 0.450256 4_1:-61836329 None:intergenic
GAAATTCTGATTACTATCAT+GGG 0.451701 4_1:+61835889 Msa0562200:CDS
ACACGATTAGACCGGGCATT+AGG 0.453044 4_1:-61835756 None:intergenic
GATTGTTGGTATTGGTTGAA+TGG 0.456384 4_1:+61835074 Msa0562200:CDS
ATCTGTGGCGTGGGGACAGA+TGG 0.457178 4_1:-61835318 None:intergenic
ATATGGCTGGGAACCTAGAA+AGG 0.461958 4_1:+61836076 Msa0562200:CDS
TCTGAGACCTCAACGCTGTC+AGG 0.466096 4_1:-61836143 None:intergenic
CGGATGACGGAATGAACACT+TGG 0.466296 4_1:-61835111 None:intergenic
CTCAAGATGAAGCCTTGAAA+AGG 0.468804 4_1:+61833953 Msa0562200:CDS
AATATATGGAAGATGAAGAT+GGG 0.468862 4_1:+61837008 Msa0562200:CDS
AGAGAGCCGCCACAGAAATT+GGG 0.468937 4_1:-61833796 None:intergenic
AACTTGGATTCTCAGCTCCT+TGG 0.469416 4_1:-61835515 None:intergenic
AATCTGAAGTTGTGGCTGTT+GGG 0.469633 4_1:-61833905 None:intergenic
TGACTCTAGCATACTCACTA+TGG 0.470886 4_1:-61835043 None:intergenic
GCATTAGGCCCATTGGTTGG+CGG 0.480720 4_1:-61835741 None:intergenic
AGAATAAATCTTCCACCAAA+TGG 0.481461 4_1:+61836314 Msa0562200:CDS
AATGAAGGAATTACCGAAAA+CGG 0.483376 4_1:+61836770 Msa0562200:CDS
CATGTACCGCCACCACCTGC+AGG 0.483658 4_1:+61835675 Msa0562200:CDS
GATGACTTTGCCAAGAAGAT+TGG 0.484215 4_1:+61837031 Msa0562200:CDS
GCCTCATGAGGGAAGGATCC+AGG 0.484648 4_1:+61836451 Msa0562200:CDS
TACCATTATAAGGCGGTGCA+TGG 0.485504 4_1:-61835359 None:intergenic
CAACCAATGGGCCTAATGCC+CGG 0.485721 4_1:+61835745 Msa0562200:CDS
TTAGGCCCATTGGTTGGCGG+TGG 0.486496 4_1:-61835738 None:intergenic
TTCTCAGAGAGTCGTCTCCT+GGG 0.488365 4_1:+61835838 Msa0562200:CDS
GAAATTCTGAAGGAGAAGAA+AGG 0.491627 4_1:+61836695 Msa0562200:CDS
TCCAAGCGCAGGAAGGGAAA+CGG 0.492405 4_1:+61836182 Msa0562200:CDS
GAATATATGGAAGATGAAGA+TGG 0.492589 4_1:+61837007 Msa0562200:CDS
GCCTCGGAAACGATTATTGA+CGG 0.493252 4_1:-61836291 None:intergenic
CAAGTGTTCATTCCGTCATC+CGG 0.493411 4_1:+61835112 Msa0562200:CDS
TTTACATGCAGCCTCTTGAC+GGG 0.494749 4_1:+61835256 Msa0562200:intron
GTCATCATGTTTGCTACTGT+GGG 0.496484 4_1:-61836741 None:intergenic
TGGATTCTCAGCTCCTTGGT+TGG 0.496891 4_1:-61835511 None:intergenic
TGGTAGGGTTTGGTCCATGC+AGG 0.497954 4_1:-61835449 None:intergenic
TCCAAGATTCAGGCTGCTGA+TGG 0.498699 4_1:+61836821 Msa0562200:CDS
GAAGCCTTCCAGATGACAAC+CGG 0.499100 4_1:-61836424 None:intergenic
CTGGGTTGGGTTGTTCGCCA+TGG 0.501508 4_1:+61833879 Msa0562200:exon
AAGGCTTCATCTTGAGGAGA+AGG 0.502963 4_1:-61833946 None:intergenic
TTATCTAACCCTGCAGGTGG+TGG 0.503622 4_1:-61835684 None:intergenic
TCATCTTGAGGAGAAGGTGT+TGG 0.504643 4_1:-61833940 None:intergenic
TGGAAAGAGGAAAAGCTTCA+GGG 0.504669 4_1:+61836619 Msa0562200:CDS
CTGAAGGAGAAGAAAGGAGT+TGG 0.505461 4_1:+61836701 Msa0562200:CDS
GTATGAGTATGAACAAGTTG+AGG 0.507239 4_1:+61836967 Msa0562200:CDS
GAGAAGAGGAGAAGAAGTAG+AGG 0.508002 4_1:-61833824 None:intergenic
AGTTCTCCACCGCCAACCAA+TGG 0.509937 4_1:+61835732 Msa0562200:CDS
TCTTTGAATGAATACGATGT+AGG 0.510886 4_1:+61835960 Msa0562200:CDS
AAAGGAGTTGGAGCTGGTGC+AGG 0.510993 4_1:+61836713 Msa0562200:CDS
GTTCTCCACCGCCAACCAAT+GGG 0.511996 4_1:+61835733 Msa0562200:CDS
CATGATGACAATAAGAATGA+AGG 0.513449 4_1:+61836755 Msa0562200:CDS
GAGTAAGAGAATGTGATGAT+GGG 0.516605 4_1:-61833711 None:intergenic
AGACCGGGCATTAGGCCCAT+TGG 0.518925 4_1:-61835748 None:intergenic
ACCAACAATCATTTGGAAAG+AGG 0.519008 4_1:+61836606 Msa0562200:CDS
TGTCATCATGTTTGCTACTG+TGG 0.520700 4_1:-61836742 None:intergenic
TCTAACCCTGCAGGTGGTGG+CGG 0.521071 4_1:-61835681 None:intergenic
CTTTGAATGAATACGATGTA+GGG 0.521877 4_1:+61835961 Msa0562200:CDS
CGGAACATTGTCAGAGAGCA+AGG 0.523049 4_1:+61836790 Msa0562200:CDS
GATCACATGCAAGAGCCATA+TGG 0.523697 4_1:+61836059 Msa0562200:CDS
GAGTCAATCCTAGAGATTGT+TGG 0.524919 4_1:+61835060 Msa0562200:CDS
TATGAGTATGAACAAGTTGA+GGG 0.525313 4_1:+61836968 Msa0562200:CDS
GTCCCTCTCTAGGTGACTGC+CGG 0.526777 4_1:-61836378 None:intergenic
GTTGCCGCAATCTGTGGCGT+GGG 0.527866 4_1:-61835327 None:intergenic
CTGTGTCTAAGTCCCTCTCT+AGG 0.527897 4_1:-61836349 None:intergenic
ACATGCAAGAGCCATATGGC+TGG 0.534478 4_1:+61836063 Msa0562200:CDS
GCAGCAGCAGCAGTTGCCGG+TGG 0.534503 4_1:-61835291 None:intergenic
GCCTCATTATCTAACCCTGC+AGG 0.535034 4_1:-61835690 None:intergenic
GTGGAAGATTTATTCTGCCT+CGG 0.535587 4_1:-61836307 None:intergenic
ACCTGCAGGGTTAGATAATG+AGG 0.535845 4_1:+61835689 Msa0562200:CDS
GCTTCCATCATATCAACTAA+AGG 0.541333 4_1:-61837355 None:intergenic
GAGAAGAAAGGAGTTGGAGC+TGG 0.544404 4_1:+61836707 Msa0562200:CDS
CTCTGTCCAAGTCCCTCTCT+AGG 0.544981 4_1:-61836388 None:intergenic
CGTTTATCCAAGCGCAGGAA+GGG 0.547030 4_1:+61836176 Msa0562200:CDS
ACCATTATAAGGCGGTGCAT+GGG 0.548023 4_1:-61835358 None:intergenic
GATGATCACAATTTCCATAG+TGG 0.554285 4_1:+61835798 Msa0562200:CDS
TGGCAGTAAAATAGCTACAC+AGG 0.556312 4_1:+61835473 Msa0562200:CDS
AGCAAGAAAGTAGACACAAT+CGG 0.559051 4_1:-61833980 None:intergenic
GATCGAATGAGGACCGAAGT+GGG 0.559665 4_1:+61836525 Msa0562200:CDS
TCCAGGATAGATTAAGAGGA+AGG 0.562641 4_1:+61836468 Msa0562200:CDS
CGGGCATTAGGCCCATTGGT+TGG 0.564557 4_1:-61835744 None:intergenic
TCCTGGATCCTTCCCTCATG+AGG 0.568928 4_1:-61836452 None:intergenic
TGTCCCCACGCCACAGATTG+CGG 0.569632 4_1:+61835323 Msa0562200:CDS
TGACTGTCACCACCATTTGG+TGG 0.569677 4_1:-61836326 None:intergenic
CGTATTCATTCAAAGAGTCT+GGG 0.569875 4_1:-61835953 None:intergenic
GACAGATGTGCCTTCCTGCA+TGG 0.570126 4_1:+61835435 Msa0562200:CDS
CCAAATCTCAATCTGAGAAG+AGG 0.573248 4_1:-61833838 None:intergenic
TTGAAAGAAAATGCAAGGCA+CGG 0.577753 4_1:-61835391 None:intergenic
CTTCAGCAGTCGATCGAATG+AGG 0.577765 4_1:+61836514 Msa0562200:CDS
CTAGAGAGGGACTTAGACAC+AGG 0.578671 4_1:+61836350 Msa0562200:CDS
TGTTGCCGCAATCTGTGGCG+TGG 0.581148 4_1:-61835328 None:intergenic
GACAGATATTACCCCAGTTG+AGG 0.582522 4_1:-61837176 None:intergenic
GCAGATGTTGCCGCAATCTG+TGG 0.582954 4_1:-61835333 None:intergenic
TCGTTTATCCAAGCGCAGGA+AGG 0.583309 4_1:+61836175 Msa0562200:CDS
GATCAGCCCAAATAGAAAGA+AGG 0.583430 4_1:+61836099 Msa0562200:CDS
GATGAATTTGGTAAAACAAG+AGG 0.583764 4_1:+61835915 Msa0562200:CDS
TCACAATTTCCATAGTGGTA+AGG 0.586818 4_1:+61835803 Msa0562200:CDS
TTGTGGCTGTTGGGCAACCA+TGG 0.587839 4_1:-61833896 None:intergenic
GAACCGGCAGTCACCTAGAG+AGG 0.588474 4_1:+61836375 Msa0562200:CDS
GTTGATGCACACGATTAGAC+CGG 0.589529 4_1:-61835764 None:intergenic
TTCAATCGCCTCATGAGGGA+AGG 0.590666 4_1:+61836444 Msa0562200:CDS
GATCATATGAGTCATAACCT+TGG 0.590754 4_1:-61836040 None:intergenic
CAGTCACCTAGAGAGGGACT+TGG 0.591478 4_1:+61836382 Msa0562200:CDS
AAGGCTTCAATCGCCTCATG+AGG 0.592807 4_1:+61836439 Msa0562200:CDS
ATCTGTCGTCGCTCATGATG+AGG 0.592954 4_1:+61836211 Msa0562200:CDS
ACCACTTAGTGAAATTCTGA+AGG 0.593072 4_1:+61836685 Msa0562200:CDS
AACCAATACCAACAATCTCT+AGG 0.593370 4_1:-61835068 None:intergenic
CGATCGAATGAGGACCGAAG+TGG 0.593485 4_1:+61836524 Msa0562200:CDS
GACATCGTTTATCCAAGCGC+AGG 0.594290 4_1:+61836171 Msa0562200:CDS
GTCTTGCTGAACTTAAGTAT+GGG 0.596178 4_1:+61836564 Msa0562200:CDS
GATCAGGAGTATGAAGGTGA+AGG 0.597456 4_1:+61836914 Msa0562200:CDS
GCCGAAGATCAGGAGTATGA+AGG 0.599647 4_1:+61836908 Msa0562200:CDS
TCTCTACCCAATTTCTGTGG+CGG 0.599837 4_1:+61833790 Msa0562200:five_prime_UTR
CATGCAAGAGCCATATGGCT+GGG 0.599944 4_1:+61836064 Msa0562200:CDS
CACGGTCGATCATCTGAGGA+AGG 0.602447 4_1:+61836866 Msa0562200:CDS
TAAAGAAACTCACCGGATGA+CGG 0.603837 4_1:-61835124 None:intergenic
CTAGAGAGGGACTTGGACAG+AGG 0.604960 4_1:+61836389 Msa0562200:CDS
CGGGTTGTTAGGAACACCAC+CGG 0.605352 4_1:+61835275 Msa0562200:CDS
TGAATATGAGCAAGGTGATG+AGG 0.606447 4_1:+61836943 Msa0562200:CDS
GTTCCTAACAACCCGTCAAG+AGG 0.607813 4_1:-61835267 None:intergenic
TATGGCTGGGAACCTAGAAA+GGG 0.609578 4_1:+61836077 Msa0562200:CDS
ATCATGTTTGCTACTGTGGG+AGG 0.610033 4_1:-61836738 None:intergenic
GATAGATTTCGTGTGCCCCA+AGG 0.610131 4_1:+61836023 Msa0562200:CDS
ACAAGAGGTCAAGATGAAAG+AGG 0.612476 4_1:+61835930 Msa0562200:CDS
ATATATGGAAGATGAAGATG+GGG 0.612975 4_1:+61837009 Msa0562200:CDS
CTCTCTCTACCCAATTTCTG+TGG 0.614772 4_1:+61833787 Msa0562200:five_prime_UTR
TAAGCATGTAGGCCTCAACT+GGG 0.615366 4_1:+61837164 Msa0562200:three_prime_UTR
TTGCTGAACTTAAGTATGGG+AGG 0.621411 4_1:+61836567 Msa0562200:CDS
GTGGTGACAGTCACCTAGAG+AGG 0.623462 4_1:+61836336 Msa0562200:CDS
TCGTAGTCCTGACAGCGTTG+AGG 0.624829 4_1:+61836136 Msa0562200:CDS
ACAGAGGAAGAATCAGTGGC+CGG 0.626828 4_1:+61836405 Msa0562200:CDS
TTGTGATCATCTGGCAAACG+AGG 0.626895 4_1:-61835786 None:intergenic
TCCGTCAATAATCGTTTCCG+AGG 0.626973 4_1:+61836290 Msa0562200:CDS
AACTCACGGTCGATCATCTG+AGG 0.627869 4_1:+61836862 Msa0562200:CDS
GACAATACTGATGTAACTCA+CGG 0.628504 4_1:+61836848 Msa0562200:CDS
TTGGCGCAGAGAACCCACTT+CGG 0.629325 4_1:-61836538 None:intergenic
GGCAGTAAAATAGCTACACA+GGG 0.630874 4_1:+61835474 Msa0562200:CDS
AGGGTTTGGTCCATGCAGGA+AGG 0.631683 4_1:-61835445 None:intergenic
AACCCAGAAGAAGAGATCGA+TGG 0.631981 4_1:-61833863 None:intergenic
TAAGGTAAAACAAGTAAGCG+TGG 0.639199 4_1:-61833685 None:intergenic
GAAGGTTGCCAGATGACGAG+AGG 0.639411 4_1:+61836486 Msa0562200:CDS
GGATGACGGAATGAACACTT+GGG 0.640470 4_1:-61835110 None:intergenic
AGGCTTCAATCGCCTCATGA+GGG 0.642774 4_1:+61836440 Msa0562200:CDS
ATGTACCGCCACCACCTGCA+GGG 0.643731 4_1:+61835676 Msa0562200:CDS
AAATTCTGATTACTATCATG+GGG 0.645440 4_1:+61835890 Msa0562200:CDS
TTGCCGCAATCTGTGGCGTG+GGG 0.646254 4_1:-61835326 None:intergenic
GTGGCCGGTTGTCATCTGGA+AGG 0.646666 4_1:+61836420 Msa0562200:CDS
CATGGACCAAACCCTACCAA+TGG 0.648418 4_1:+61835453 Msa0562200:CDS
ATAAATCTTCCACCAAATGG+TGG 0.648459 4_1:+61836317 Msa0562200:CDS
TTGGACAGAGGAAGAATCAG+TGG 0.649407 4_1:+61836401 Msa0562200:CDS
GGAGACTATGAATATGAGCA+AGG 0.650461 4_1:+61836935 Msa0562200:CDS
TTGATGCACACGATTAGACC+GGG 0.654044 4_1:-61835763 None:intergenic
TGCGCTTGGATAAACGATGT+CGG 0.656360 4_1:-61836169 None:intergenic
TCAATGACAATGACCAACCA+AGG 0.673022 4_1:+61835498 Msa0562200:CDS
AACCGGCAGTCACCTAGAGA+GGG 0.673757 4_1:+61836376 Msa0562200:CDS
TGAGTCTGAATCTGAAGTTG+TGG 0.677604 4_1:-61833913 None:intergenic
TGGTGACAGTCACCTAGAGA+GGG 0.684816 4_1:+61836337 Msa0562200:CDS
TCATTATCTAACCCTGCAGG+TGG 0.685323 4_1:-61835687 None:intergenic
TGTTTACTACCATTATAAGG+CGG 0.686484 4_1:-61835366 None:intergenic
TCATATGAGTCATAACCTTG+GGG 0.704228 4_1:-61836038 None:intergenic
CTCTAGCATACTCACTATGG+CGG 0.707134 4_1:-61835040 None:intergenic
GCTACAAGAACATCAAACCC+AGG 0.714968 4_1:-61835855 None:intergenic
AAGCATGTAGGCCTCAACTG+GGG 0.762858 4_1:+61837165 Msa0562200:three_prime_UTR
GCAGTAAAATAGCTACACAG+GGG 0.802739 4_1:+61835475 Msa0562200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTAATAATAATA+GGG - chr4_1:61834655-61834674 None:intergenic 0.0%
!! ATAATTGATAAAGAAAAAAA+GGG - chr4_1:61834059-61834078 None:intergenic 10.0%
!! TATAATTGATAAAGAAAAAA+AGG - chr4_1:61834060-61834079 None:intergenic 10.0%
!!! TTTATCAATTATATCTTTTA+GGG + chr4_1:61834067-61834086 Msa0562200:intron 10.0%
!!! AAATTTAATTTTGATTAATG+GGG + chr4_1:61834149-61834168 Msa0562200:intron 10.0%
!!! CTTTATCAATTATATCTTTT+AGG + chr4_1:61834066-61834085 Msa0562200:intron 15.0%
!! CATAACTCAATTAAAATAAA+AGG - chr4_1:61835168-61835187 None:intergenic 15.0%
!!! TTGTTCTTTCATAATTTTTA+TGG + chr4_1:61837056-61837075 Msa0562200:exon 15.0%
!!! TCTACAATTCAACAATTTTA+GGG - chr4_1:61834211-61834230 None:intergenic 20.0%
!!! ATCTACAATTCAACAATTTT+AGG - chr4_1:61834212-61834231 None:intergenic 20.0%
!! AATACTCTAAACTTAATGAA+AGG - chr4_1:61834564-61834583 None:intergenic 20.0%
!! CATCTGTTATTAAGAATATA+AGG + chr4_1:61834918-61834937 Msa0562200:intron 20.0%
!!! AACTTAAACATATTTTTCTG+TGG - chr4_1:61835648-61835667 None:intergenic 20.0%
!! ACAGAAAAATATGTTTAAGT+TGG + chr4_1:61835647-61835666 Msa0562200:CDS 20.0%
!!! TGATATTTTTATGTAAGAAG+TGG + chr4_1:61837249-61837268 Msa0562200:three_prime_UTR 20.0%
!!! GATATTTTTATGTAAGAAGT+GGG + chr4_1:61837250-61837269 Msa0562200:three_prime_UTR 20.0%
!!! ATATTTTTATGTAAGAAGTG+GGG + chr4_1:61837251-61837270 Msa0562200:three_prime_UTR 20.0%
!!! AAAAAGGGCTTTTTAGAAAA+AGG - chr4_1:61834044-61834063 None:intergenic 25.0%
!!! TTTCAGATTCGAATTAGTTT+TGG + chr4_1:61834090-61834109 Msa0562200:intron 25.0%
!!! TAATTTTGATTAATGGGGTT+TGG + chr4_1:61834154-61834173 Msa0562200:intron 25.0%
! ACAAAAAAATCTTCATGGAT+GGG - chr4_1:61834237-61834256 None:intergenic 25.0%
! AACAAAAAAATCTTCATGGA+TGG - chr4_1:61834238-61834257 None:intergenic 25.0%
! GTCTAACAAAAAAATCTTCA+TGG - chr4_1:61834242-61834261 None:intergenic 25.0%
!!! GTTAGACTGTGAATTTTTTA+TGG + chr4_1:61834255-61834274 Msa0562200:intron 25.0%
!!! TGATCATCTTGTTATTTGAT+TGG + chr4_1:61834305-61834324 Msa0562200:intron 25.0%
!! TCCTTTTGTATTTAACATAG+TGG + chr4_1:61834365-61834384 Msa0562200:intron 25.0%
!! CCTTTTGTATTTAACATAGT+GGG + chr4_1:61834366-61834385 Msa0562200:intron 25.0%
! AATAAGCTATTTATCCAAAC+AGG + chr4_1:61834628-61834647 Msa0562200:intron 25.0%
! ATAAGCTATTTATCCAAACA+GGG + chr4_1:61834629-61834648 Msa0562200:intron 25.0%
! AGTATTTGAGTCTTGATTAT+AGG + chr4_1:61834687-61834706 Msa0562200:intron 25.0%
! TTCTCTTCTTTATTGAAACA+GGG + chr4_1:61835002-61835021 Msa0562200:intron 25.0%
! AGAAATTCTGATTACTATCA+TGG + chr4_1:61835888-61835907 Msa0562200:CDS 25.0%
! GAAATTCTGATTACTATCAT+GGG + chr4_1:61835889-61835908 Msa0562200:CDS 25.0%
! AAATTCTGATTACTATCATG+GGG + chr4_1:61835890-61835909 Msa0562200:CDS 25.0%
! AATAATGACCAACAATCATT+TGG + chr4_1:61836599-61836618 Msa0562200:CDS 25.0%
! AAATCAAGAGCAAGAATATA+TGG + chr4_1:61836994-61837013 Msa0562200:CDS 25.0%
! AATATATGGAAGATGAAGAT+GGG + chr4_1:61837008-61837027 Msa0562200:CDS 25.0%
! CAAGTTAAAGAGACAATATA+AGG - chr4_1:61837128-61837147 None:intergenic 25.0%
! CTTATATTGTCTCTTTAACT+TGG + chr4_1:61837126-61837145 Msa0562200:three_prime_UTR 25.0%
!!! TTTTCCTTTAGTTGATATGA+TGG + chr4_1:61837351-61837370 Msa0562200:three_prime_UTR 25.0%
!! TTTTTATGGATTGCGTTTCT+TGG + chr4_1:61834269-61834288 Msa0562200:intron 30.0%
AAAGGAATGATCAAAAAGAG+AGG - chr4_1:61834351-61834370 None:intergenic 30.0%
CCCACTATGTTAAATACAAA+AGG - chr4_1:61834369-61834388 None:intergenic 30.0%
!!! TGTGTTTTTTTGAGTAATCC+TGG + chr4_1:61834737-61834756 Msa0562200:intron 30.0%
AGTTAACAAAAACAACTTCC+AGG - chr4_1:61834758-61834777 None:intergenic 30.0%
!!! ACATGCTGCTTTTATTTTTG+TGG + chr4_1:61834847-61834866 Msa0562200:intron 30.0%
GTTCTCTTCTTTATTGAAAC+AGG + chr4_1:61835001-61835020 Msa0562200:intron 30.0%
AGTCAAAAGATAGGACAAAA+TGG - chr4_1:61835203-61835222 None:intergenic 30.0%
ATACAGACAAGTCAAAAGAT+AGG - chr4_1:61835212-61835231 None:intergenic 30.0%
!!! TATCTTTTGACTTGTCTGTA+TGG + chr4_1:61835211-61835230 Msa0562200:intron 30.0%
TGTTTACTACCATTATAAGG+CGG - chr4_1:61835369-61835388 None:intergenic 30.0%
GCTTGTTTACTACCATTATA+AGG - chr4_1:61835372-61835391 None:intergenic 30.0%
! CCTTGCATTTTCTTTCAAAA+GGG + chr4_1:61835396-61835415 Msa0562200:CDS 30.0%
! CTTGCATTTTCTTTCAAAAG+GGG + chr4_1:61835397-61835416 Msa0562200:CDS 30.0%
CCAAAAGGTTTCTTAAAACT+TGG - chr4_1:61835534-61835553 None:intergenic 30.0%
!!! CCAAGTTTTAAGAAACCTTT+TGG + chr4_1:61835531-61835550 Msa0562200:CDS 30.0%
!!! AGTTTTAAGAAACCTTTTGG+CGG + chr4_1:61835534-61835553 Msa0562200:CDS 30.0%
GATGAATTTGGTAAAACAAG+AGG + chr4_1:61835915-61835934 Msa0562200:CDS 30.0%
TCTTTGAATGAATACGATGT+AGG + chr4_1:61835960-61835979 Msa0562200:CDS 30.0%
CTTTGAATGAATACGATGTA+GGG + chr4_1:61835961-61835980 Msa0562200:CDS 30.0%
ATCATATGAGTCATAACCTT+GGG - chr4_1:61836042-61836061 None:intergenic 30.0%
ATGAGTCCTTCTTTCTATTT+GGG - chr4_1:61836108-61836127 None:intergenic 30.0%
AGAATAAATCTTCCACCAAA+TGG + chr4_1:61836314-61836333 Msa0562200:CDS 30.0%
! TTAAGTTCAGCAAGACTTTT+TGG - chr4_1:61836560-61836579 None:intergenic 30.0%
GTACATCATCTTCATATTGT+TGG - chr4_1:61836649-61836668 None:intergenic 30.0%
! AGAATTTCACTAAGTGGTTT+TGG - chr4_1:61836683-61836702 None:intergenic 30.0%
CATGATGACAATAAGAATGA+AGG + chr4_1:61836755-61836774 Msa0562200:CDS 30.0%
AATGAAGGAATTACCGAAAA+CGG + chr4_1:61836770-61836789 Msa0562200:CDS 30.0%
! TATGAGTATGAACAAGTTGA+GGG + chr4_1:61836968-61836987 Msa0562200:CDS 30.0%
GAATATATGGAAGATGAAGA+TGG + chr4_1:61837007-61837026 Msa0562200:CDS 30.0%
ATATATGGAAGATGAAGATG+GGG + chr4_1:61837009-61837028 Msa0562200:CDS 30.0%
!!! TTTTTTCTGCTTAAGCATGT+AGG + chr4_1:61837153-61837172 Msa0562200:three_prime_UTR 30.0%
TATTAACTCTCTAACGTTAC+AGG - chr4_1:61837308-61837327 None:intergenic 30.0%
TAAAACAAGTAAGCGTGGTT+TGG - chr4_1:61833683-61833702 None:intergenic 35.0%
TAAGGTAAAACAAGTAAGCG+TGG - chr4_1:61833688-61833707 None:intergenic 35.0%
GAGTAAGAGAATGTGATGAT+GGG - chr4_1:61833714-61833733 None:intergenic 35.0%
AGAGTAAGAGAATGTGATGA+TGG - chr4_1:61833715-61833734 None:intergenic 35.0%
AGCAAGAAAGTAGACACAAT+CGG - chr4_1:61833983-61834002 None:intergenic 35.0%
! AGAACCTTTTTGCATGTCAA+GGG - chr4_1:61834009-61834028 None:intergenic 35.0%
! AAGAACCTTTTTGCATGTCA+AGG - chr4_1:61834010-61834029 None:intergenic 35.0%
!! GTATTTAACATAGTGGGTGA+AGG + chr4_1:61834372-61834391 Msa0562200:intron 35.0%
GTGAAGGAAATTAGGATGAA+CGG + chr4_1:61834388-61834407 Msa0562200:intron 35.0%
TGAAGGAAATTAGGATGAAC+GGG + chr4_1:61834389-61834408 Msa0562200:intron 35.0%
GAACCCTAATTGTGATTGTT+AGG + chr4_1:61834418-61834437 Msa0562200:intron 35.0%
GGTCCTAACAATCACAATTA+GGG - chr4_1:61834424-61834443 None:intergenic 35.0%
TGGTCCTAACAATCACAATT+AGG - chr4_1:61834425-61834444 None:intergenic 35.0%
CATGTGTATAGTGTAGTGAA+TGG + chr4_1:61834538-61834557 Msa0562200:intron 35.0%
AATAATAATAGGGCCCTGTT+TGG - chr4_1:61834645-61834664 None:intergenic 35.0%
ACTGCTAACAAACACTATAC+AGG - chr4_1:61834717-61834736 None:intergenic 35.0%
ATCCTAGAGATTGTTGGTAT+TGG + chr4_1:61835066-61835085 Msa0562200:CDS 35.0%
AACCAATACCAACAATCTCT+AGG - chr4_1:61835071-61835090 None:intergenic 35.0%
!! GATTGTTGGTATTGGTTGAA+TGG + chr4_1:61835074-61835093 Msa0562200:CDS 35.0%
TTGAAAGAAAATGCAAGGCA+CGG - chr4_1:61835394-61835413 None:intergenic 35.0%
! GCCTTGCATTTTCTTTCAAA+AGG + chr4_1:61835395-61835414 Msa0562200:CDS 35.0%
!! CCCTTTTGAAAGAAAATGCA+AGG - chr4_1:61835399-61835418 None:intergenic 35.0%
! CAAAAGGGGTTTTGCTTAAA+AGG + chr4_1:61835411-61835430 Msa0562200:CDS 35.0%
! CTATTTTACTGCCATTGGTA+GGG - chr4_1:61835467-61835486 None:intergenic 35.0%
!! TGTAGCTATTTTACTGCCAT+TGG - chr4_1:61835472-61835491 None:intergenic 35.0%
TTGAAAAACATGCACCAGAA+AGG + chr4_1:61835559-61835578 Msa0562200:CDS 35.0%
CTATGGAAATTGTGATCATC+TGG - chr4_1:61835798-61835817 None:intergenic 35.0%
GATGATCACAATTTCCATAG+TGG + chr4_1:61835798-61835817 Msa0562200:CDS 35.0%
TCACAATTTCCATAGTGGTA+AGG + chr4_1:61835803-61835822 Msa0562200:CDS 35.0%
TATCATGGGGAAGATGAATT+TGG + chr4_1:61835903-61835922 Msa0562200:CDS 35.0%
CGTATTCATTCAAAGAGTCT+GGG - chr4_1:61835956-61835975 None:intergenic 35.0%
TCGTATTCATTCAAAGAGTC+TGG - chr4_1:61835957-61835976 None:intergenic 35.0%
TCATATGAGTCATAACCTTG+GGG - chr4_1:61836041-61836060 None:intergenic 35.0%
GATCATATGAGTCATAACCT+TGG - chr4_1:61836043-61836062 None:intergenic 35.0%
GATGAGTCCTTCTTTCTATT+TGG - chr4_1:61836109-61836128 None:intergenic 35.0%
!!! AGCAAAGTCATCGATTTTCT+CGG + chr4_1:61836234-61836253 Msa0562200:CDS 35.0%
ATAAATCTTCCACCAAATGG+TGG + chr4_1:61836317-61836336 Msa0562200:CDS 35.0%
AGTCTTGCTGAACTTAAGTA+TGG + chr4_1:61836563-61836582 Msa0562200:CDS 35.0%
GTCTTGCTGAACTTAAGTAT+GGG + chr4_1:61836564-61836583 Msa0562200:CDS 35.0%
ACCAACAATCATTTGGAAAG+AGG + chr4_1:61836606-61836625 Msa0562200:CDS 35.0%
TCCTCTTTCCAAATGATTGT+TGG - chr4_1:61836610-61836629 None:intergenic 35.0%
GAAGATGATGTACCATTTGA+AGG + chr4_1:61836656-61836675 Msa0562200:CDS 35.0%
ACCACTTAGTGAAATTCTGA+AGG + chr4_1:61836685-61836704 Msa0562200:CDS 35.0%
TCCTTCAGAATTTCACTAAG+TGG - chr4_1:61836689-61836708 None:intergenic 35.0%
! GAAATTCTGAAGGAGAAGAA+AGG + chr4_1:61836695-61836714 Msa0562200:CDS 35.0%
GACAATACTGATGTAACTCA+CGG + chr4_1:61836848-61836867 Msa0562200:CDS 35.0%
GTATGAGTATGAACAAGTTG+AGG + chr4_1:61836967-61836986 Msa0562200:CDS 35.0%
AAGAACAACACCAATCTTCT+TGG - chr4_1:61837044-61837063 None:intergenic 35.0%
GCTTCCATCATATCAACTAA+AGG - chr4_1:61837358-61837377 None:intergenic 35.0%
!! GAATGTGATGATGGGCTTTA+AGG - chr4_1:61833706-61833725 None:intergenic 40.0%
CCAAATCTCAATCTGAGAAG+AGG - chr4_1:61833841-61833860 None:intergenic 40.0%
CCTCTTCTCAGATTGAGATT+TGG + chr4_1:61833838-61833857 Msa0562200:five_prime_UTR 40.0%
ATCCATCGATCTCTTCTTCT+GGG + chr4_1:61833861-61833880 Msa0562200:five_prime_UTR 40.0%
! AATCTGAAGTTGTGGCTGTT+GGG - chr4_1:61833908-61833927 None:intergenic 40.0%
! TGAGTCTGAATCTGAAGTTG+TGG - chr4_1:61833916-61833935 None:intergenic 40.0%
!! CTTTTCAAGGCTTCATCTTG+AGG - chr4_1:61833955-61833974 None:intergenic 40.0%
CTCAAGATGAAGCCTTGAAA+AGG + chr4_1:61833953-61833972 Msa0562200:CDS 40.0%
!! ACAATCGGTGTTCCTTTTCA+AGG - chr4_1:61833968-61833987 None:intergenic 40.0%
TTCTCCCTTGACATGCAAAA+AGG + chr4_1:61834002-61834021 Msa0562200:CDS 40.0%
!! CATAGTGGGTGAAGGAAATT+AGG + chr4_1:61834380-61834399 Msa0562200:intron 40.0%
GAAGGAAATTAGGATGAACG+GGG + chr4_1:61834390-61834409 Msa0562200:intron 40.0%
! GACATCAGCTTACACTTTTG+TGG - chr4_1:61834493-61834512 None:intergenic 40.0%
GCTGATGTCAATTGAGCATT+TGG + chr4_1:61834504-61834523 Msa0562200:intron 40.0%
TGACTCTAGCATACTCACTA+TGG - chr4_1:61835046-61835065 None:intergenic 40.0%
GAGTCAATCCTAGAGATTGT+TGG + chr4_1:61835060-61835079 Msa0562200:CDS 40.0%
TAAAGAAACTCACCGGATGA+CGG - chr4_1:61835127-61835146 None:intergenic 40.0%
! TTTTACATGCAGCCTCTTGA+CGG + chr4_1:61835255-61835274 Msa0562200:intron 40.0%
!! GCTATTTTACTGCCATTGGT+AGG - chr4_1:61835468-61835487 None:intergenic 40.0%
TGGCAGTAAAATAGCTACAC+AGG + chr4_1:61835473-61835492 Msa0562200:CDS 40.0%
GGCAGTAAAATAGCTACACA+GGG + chr4_1:61835474-61835493 Msa0562200:CDS 40.0%
GCAGTAAAATAGCTACACAG+GGG + chr4_1:61835475-61835494 Msa0562200:CDS 40.0%
TCAATGACAATGACCAACCA+AGG + chr4_1:61835498-61835517 Msa0562200:CDS 40.0%
!! TGTTTTTCAATGCCGCCAAA+AGG - chr4_1:61835549-61835568 None:intergenic 40.0%
AACACCTGCAACATTTGCTT+GGG - chr4_1:61835592-61835611 None:intergenic 40.0%
!! GCAGTCTCAAATTTTTGGAC+AGG - chr4_1:61835624-61835643 None:intergenic 40.0%
! GTGGAGCAGTCTCAAATTTT+TGG - chr4_1:61835629-61835648 None:intergenic 40.0%
ACAAGAGGTCAAGATGAAAG+AGG + chr4_1:61835930-61835949 Msa0562200:CDS 40.0%
GATCAGCCCAAATAGAAAGA+AGG + chr4_1:61836099-61836118 Msa0562200:CDS 40.0%
!!! AAGTCATCGATTTTCTCGGA+AGG + chr4_1:61836238-61836257 Msa0562200:CDS 40.0%
GTGGAAGATTTATTCTGCCT+CGG - chr4_1:61836310-61836329 None:intergenic 40.0%
AGGATCCAGGATAGATTAAG+AGG + chr4_1:61836464-61836483 Msa0562200:CDS 40.0%
TCCAGGATAGATTAAGAGGA+AGG + chr4_1:61836468-61836487 Msa0562200:CDS 40.0%
ACCTTCCTCTTAATCTATCC+TGG - chr4_1:61836472-61836491 None:intergenic 40.0%
TTGCTGAACTTAAGTATGGG+AGG + chr4_1:61836567-61836586 Msa0562200:CDS 40.0%
TTGGAAAGAGGAAAAGCTTC+AGG + chr4_1:61836618-61836637 Msa0562200:CDS 40.0%
TGGAAAGAGGAAAAGCTTCA+GGG + chr4_1:61836619-61836638 Msa0562200:CDS 40.0%
!!! AGTGGTTTTGGACCTTCAAA+TGG - chr4_1:61836671-61836690 None:intergenic 40.0%
! GTCATCATGTTTGCTACTGT+GGG - chr4_1:61836744-61836763 None:intergenic 40.0%
! TGTCATCATGTTTGCTACTG+TGG - chr4_1:61836745-61836764 None:intergenic 40.0%
! CTCTGACAATGTTCCGTTTT+CGG - chr4_1:61836786-61836805 None:intergenic 40.0%
ACCTTCATACTCCTGATCTT+CGG - chr4_1:61836912-61836931 None:intergenic 40.0%
GGAGACTATGAATATGAGCA+AGG + chr4_1:61836935-61836954 Msa0562200:CDS 40.0%
TGAATATGAGCAAGGTGATG+AGG + chr4_1:61836943-61836962 Msa0562200:CDS 40.0%
GATGACTTTGCCAAGAAGAT+TGG + chr4_1:61837031-61837050 Msa0562200:CDS 40.0%
CTCTCTCTACCCAATTTCTG+TGG + chr4_1:61833787-61833806 Msa0562200:five_prime_UTR 45.0%
TCTCTACCCAATTTCTGTGG+CGG + chr4_1:61833790-61833809 Msa0562200:five_prime_UTR 45.0%
GAGAAGAGGAGAAGAAGTAG+AGG - chr4_1:61833827-61833846 None:intergenic 45.0%
GATCCATCGATCTCTTCTTC+TGG + chr4_1:61833860-61833879 Msa0562200:five_prime_UTR 45.0%
AACCCAGAAGAAGAGATCGA+TGG - chr4_1:61833866-61833885 None:intergenic 45.0%
ATCGATCTCTTCTTCTGGGT+TGG + chr4_1:61833865-61833884 Msa0562200:five_prime_UTR 45.0%
TCGATCTCTTCTTCTGGGTT+GGG + chr4_1:61833866-61833885 Msa0562200:five_prime_UTR 45.0%
! GAATCTGAAGTTGTGGCTGT+TGG - chr4_1:61833909-61833928 None:intergenic 45.0%
! TCATCTTGAGGAGAAGGTGT+TGG - chr4_1:61833943-61833962 None:intergenic 45.0%
AAGGCTTCATCTTGAGGAGA+AGG - chr4_1:61833949-61833968 None:intergenic 45.0%
CTCTAGCATACTCACTATGG+CGG - chr4_1:61835043-61835062 None:intergenic 45.0%
!! GGATGACGGAATGAACACTT+GGG - chr4_1:61835113-61835132 None:intergenic 45.0%
CAAGTGTTCATTCCGTCATC+CGG + chr4_1:61835112-61835131 Msa0562200:CDS 45.0%
! GTGCTGGTAAAGAAACTCAC+CGG - chr4_1:61835134-61835153 None:intergenic 45.0%
AAAGGACAGAAAACTCGTGC+TGG - chr4_1:61835150-61835169 None:intergenic 45.0%
TTTACATGCAGCCTCTTGAC+GGG + chr4_1:61835256-61835275 Msa0562200:intron 45.0%
ACCATTATAAGGCGGTGCAT+GGG - chr4_1:61835361-61835380 None:intergenic 45.0%
TACCATTATAAGGCGGTGCA+TGG - chr4_1:61835362-61835381 None:intergenic 45.0%
TTACTGCCATTGGTAGGGTT+TGG - chr4_1:61835462-61835481 None:intergenic 45.0%
AACTTGGATTCTCAGCTCCT+TGG - chr4_1:61835518-61835537 None:intergenic 45.0%
! GCTTGGGAAGTTTTCCTTTC+TGG - chr4_1:61835576-61835595 None:intergenic 45.0%
ACTTCCCAAGCAAATGTTGC+AGG + chr4_1:61835585-61835604 Msa0562200:CDS 45.0%
CAACACCTGCAACATTTGCT+TGG - chr4_1:61835593-61835612 None:intergenic 45.0%
TCATTATCTAACCCTGCAGG+TGG - chr4_1:61835690-61835709 None:intergenic 45.0%
ACCTGCAGGGTTAGATAATG+AGG + chr4_1:61835689-61835708 Msa0562200:CDS 45.0%
TTGATGCACACGATTAGACC+GGG - chr4_1:61835766-61835785 None:intergenic 45.0%
GTTGATGCACACGATTAGAC+CGG - chr4_1:61835767-61835786 None:intergenic 45.0%
TTGTGATCATCTGGCAAACG+AGG - chr4_1:61835789-61835808 None:intergenic 45.0%
CGTCATTGTCCTTACCACTA+TGG - chr4_1:61835815-61835834 None:intergenic 45.0%
GCTACAAGAACATCAAACCC+AGG - chr4_1:61835858-61835877 None:intergenic 45.0%
GATCACATGCAAGAGCCATA+TGG + chr4_1:61836059-61836078 Msa0562200:CDS 45.0%
TTTCTAGGTTCCCAGCCATA+TGG - chr4_1:61836077-61836096 None:intergenic 45.0%
ATATGGCTGGGAACCTAGAA+AGG + chr4_1:61836076-61836095 Msa0562200:CDS 45.0%
TATGGCTGGGAACCTAGAAA+GGG + chr4_1:61836077-61836096 Msa0562200:CDS 45.0%
ATTTGGGCTGATCCCTTTCT+AGG - chr4_1:61836092-61836111 None:intergenic 45.0%
TGCGCTTGGATAAACGATGT+CGG - chr4_1:61836172-61836191 None:intergenic 45.0%
ATCTCTCATTTGCAGGCAAC+TGG - chr4_1:61836271-61836290 None:intergenic 45.0%
! TTGACGGATCTCTCATTTGC+AGG - chr4_1:61836278-61836297 None:intergenic 45.0%
TCCGTCAATAATCGTTTCCG+AGG + chr4_1:61836290-61836309 Msa0562200:CDS 45.0%
GCCTCGGAAACGATTATTGA+CGG - chr4_1:61836294-61836313 None:intergenic 45.0%
GACTTAGACACAGGAAGAAC+CGG + chr4_1:61836359-61836378 Msa0562200:CDS 45.0%
TTGGACAGAGGAAGAATCAG+TGG + chr4_1:61836401-61836420 Msa0562200:CDS 45.0%
AAGTTTCTCCTCTCGTCATC+TGG - chr4_1:61836497-61836516 None:intergenic 45.0%
CTGAAGGAGAAGAAAGGAGT+TGG + chr4_1:61836701-61836720 Msa0562200:CDS 45.0%
! ATCATGTTTGCTACTGTGGG+AGG - chr4_1:61836741-61836760 None:intergenic 45.0%
GGAAGAAGAGTCCAAGATTC+AGG + chr4_1:61836811-61836830 Msa0562200:CDS 45.0%
! GATCAGGAGTATGAAGGTGA+AGG + chr4_1:61836914-61836933 Msa0562200:CDS 45.0%
TTAAGCATGTAGGCCTCAAC+TGG + chr4_1:61837163-61837182 Msa0562200:three_prime_UTR 45.0%
TAAGCATGTAGGCCTCAACT+GGG + chr4_1:61837164-61837183 Msa0562200:three_prime_UTR 45.0%
GACAGATATTACCCCAGTTG+AGG - chr4_1:61837179-61837198 None:intergenic 45.0%
!!! AAAAATTTAATTTTGATTAA+TGG + chr4_1:61834147-61834166 Msa0562200:intron 5.0%
!!! AAAATTTAATTTTGATTAAT+GGG + chr4_1:61834148-61834167 Msa0562200:intron 5.0%
!! TAATAAAATAACAAAAATTA+TGG - chr4_1:61834445-61834464 None:intergenic 5.0%
!!! CTTTTTATTTTAATAATAAT+AGG - chr4_1:61834656-61834675 None:intergenic 5.0%
GAGAGAGAGAGAGAGAGTAG+AGG - chr4_1:61833772-61833791 None:intergenic 50.0%
AGAGAGCCGCCACAGAAATT+GGG - chr4_1:61833799-61833818 None:intergenic 50.0%
TTGTGGCTGTGCGAATCATG+TGG + chr4_1:61834864-61834883 Msa0562200:intron 50.0%
!! CGGATGACGGAATGAACACT+TGG - chr4_1:61835114-61835133 None:intergenic 50.0%
GTTCCTAACAACCCGTCAAG+AGG - chr4_1:61835270-61835289 None:intergenic 50.0%
ACCCATGCACCGCCTTATAA+TGG + chr4_1:61835357-61835376 Msa0562200:CDS 50.0%
CATGGACCAAACCCTACCAA+TGG + chr4_1:61835453-61835472 Msa0562200:CDS 50.0%
TGGATTCTCAGCTCCTTGGT+TGG - chr4_1:61835514-61835533 None:intergenic 50.0%
TTATCTAACCCTGCAGGTGG+TGG - chr4_1:61835687-61835706 None:intergenic 50.0%
GCCTCATTATCTAACCCTGC+AGG - chr4_1:61835693-61835712 None:intergenic 50.0%
ACACGATTAGACCGGGCATT+AGG - chr4_1:61835759-61835778 None:intergenic 50.0%
TTTCTCAGAGAGTCGTCTCC+TGG + chr4_1:61835837-61835856 Msa0562200:CDS 50.0%
TTCTCAGAGAGTCGTCTCCT+GGG + chr4_1:61835838-61835857 Msa0562200:CDS 50.0%
GATAGATTTCGTGTGCCCCA+AGG + chr4_1:61836023-61836042 Msa0562200:CDS 50.0%
ACATGCAAGAGCCATATGGC+TGG + chr4_1:61836063-61836082 Msa0562200:CDS 50.0%
CATGCAAGAGCCATATGGCT+GGG + chr4_1:61836064-61836083 Msa0562200:CDS 50.0%
GACATCGTTTATCCAAGCGC+AGG + chr4_1:61836171-61836190 Msa0562200:CDS 50.0%
TCGTTTATCCAAGCGCAGGA+AGG + chr4_1:61836175-61836194 Msa0562200:CDS 50.0%
CGTTTATCCAAGCGCAGGAA+GGG + chr4_1:61836176-61836195 Msa0562200:CDS 50.0%
ATCTGTCGTCGCTCATGATG+AGG + chr4_1:61836211-61836230 Msa0562200:CDS 50.0%
TGACTGTCACCACCATTTGG+TGG - chr4_1:61836329-61836348 None:intergenic 50.0%
AGGTGACTGTCACCACCATT+TGG - chr4_1:61836332-61836351 None:intergenic 50.0%
TGGTGACAGTCACCTAGAGA+GGG + chr4_1:61836337-61836356 Msa0562200:CDS 50.0%
CTGTGTCTAAGTCCCTCTCT+AGG - chr4_1:61836352-61836371 None:intergenic 50.0%
CTAGAGAGGGACTTAGACAC+AGG + chr4_1:61836350-61836369 Msa0562200:CDS 50.0%
ACAGAGGAAGAATCAGTGGC+CGG + chr4_1:61836405-61836424 Msa0562200:CDS 50.0%
GAAGCCTTCCAGATGACAAC+CGG - chr4_1:61836427-61836446 None:intergenic 50.0%
AAGGCTTCAATCGCCTCATG+AGG + chr4_1:61836439-61836458 Msa0562200:CDS 50.0%
AGGCTTCAATCGCCTCATGA+GGG + chr4_1:61836440-61836459 Msa0562200:CDS 50.0%
TTCAATCGCCTCATGAGGGA+AGG + chr4_1:61836444-61836463 Msa0562200:CDS 50.0%
CTTCAGCAGTCGATCGAATG+AGG + chr4_1:61836514-61836533 Msa0562200:CDS 50.0%
GATCGAATGAGGACCGAAGT+GGG + chr4_1:61836525-61836544 Msa0562200:CDS 50.0%
GAGAAGAAAGGAGTTGGAGC+TGG + chr4_1:61836707-61836726 Msa0562200:CDS 50.0%
CGGAACATTGTCAGAGAGCA+AGG + chr4_1:61836790-61836809 Msa0562200:CDS 50.0%
TCCAAGATTCAGGCTGCTGA+TGG + chr4_1:61836821-61836840 Msa0562200:CDS 50.0%
TCCATCAGCAGCCTGAATCT+TGG - chr4_1:61836825-61836844 None:intergenic 50.0%
AACTCACGGTCGATCATCTG+AGG + chr4_1:61836862-61836881 Msa0562200:CDS 50.0%
! GCCGAAGATCAGGAGTATGA+AGG + chr4_1:61836908-61836927 Msa0562200:CDS 50.0%
AAGCATGTAGGCCTCAACTG+GGG + chr4_1:61837165-61837184 Msa0562200:three_prime_UTR 50.0%
GAGAGAGCCGCCACAGAAAT+TGG - chr4_1:61833800-61833819 None:intergenic 55.0%
TTGTGGCTGTTGGGCAACCA+TGG - chr4_1:61833899-61833918 None:intergenic 55.0%
!! CAGCCTCTTGACGGGTTGTT+AGG + chr4_1:61835264-61835283 Msa0562200:intron 55.0%
CGGGTTGTTAGGAACACCAC+CGG + chr4_1:61835275-61835294 Msa0562200:CDS 55.0%
GCAGATGTTGCCGCAATCTG+TGG - chr4_1:61835336-61835355 None:intergenic 55.0%
GACAGATGTGCCTTCCTGCA+TGG + chr4_1:61835435-61835454 Msa0562200:CDS 55.0%
! AGGGTTTGGTCCATGCAGGA+AGG - chr4_1:61835448-61835467 None:intergenic 55.0%
! TGGTAGGGTTTGGTCCATGC+AGG - chr4_1:61835452-61835471 None:intergenic 55.0%
AGTTCTCCACCGCCAACCAA+TGG + chr4_1:61835732-61835751 Msa0562200:CDS 55.0%
GTTCTCCACCGCCAACCAAT+GGG + chr4_1:61835733-61835752 Msa0562200:CDS 55.0%
! GCATTAGGCCCATTGGTTGG+CGG - chr4_1:61835744-61835763 None:intergenic 55.0%
CAACCAATGGGCCTAATGCC+CGG + chr4_1:61835745-61835764 Msa0562200:CDS 55.0%
TCGTAGTCCTGACAGCGTTG+AGG + chr4_1:61836136-61836155 Msa0562200:CDS 55.0%
! TCTGAGACCTCAACGCTGTC+AGG - chr4_1:61836146-61836165 None:intergenic 55.0%
TCCAAGCGCAGGAAGGGAAA+CGG + chr4_1:61836182-61836201 Msa0562200:CDS 55.0%
GTGGTGACAGTCACCTAGAG+AGG + chr4_1:61836336-61836355 Msa0562200:CDS 55.0%
AACCGGCAGTCACCTAGAGA+GGG + chr4_1:61836376-61836395 Msa0562200:CDS 55.0%
CAGTCACCTAGAGAGGGACT+TGG + chr4_1:61836343-61836362 Msa0562200:CDS 55.0%
CTCTGTCCAAGTCCCTCTCT+AGG - chr4_1:61836391-61836410 None:intergenic 55.0%
CTAGAGAGGGACTTGGACAG+AGG + chr4_1:61836389-61836408 Msa0562200:CDS 55.0%
ATCAGTGGCCGGTTGTCATC+TGG + chr4_1:61836416-61836435 Msa0562200:CDS 55.0%
TCCTGGATCCTTCCCTCATG+AGG - chr4_1:61836455-61836474 None:intergenic 55.0%
GAAGGTTGCCAGATGACGAG+AGG + chr4_1:61836486-61836505 Msa0562200:CDS 55.0%
CGATCGAATGAGGACCGAAG+TGG + chr4_1:61836524-61836543 Msa0562200:CDS 55.0%
TTGGCGCAGAGAACCCACTT+CGG - chr4_1:61836541-61836560 None:intergenic 55.0%
AAAGGAGTTGGAGCTGGTGC+AGG + chr4_1:61836713-61836732 Msa0562200:CDS 55.0%
CACGGTCGATCATCTGAGGA+AGG + chr4_1:61836866-61836885 Msa0562200:CDS 55.0%
CGATGAAGCTGCCGAAGATC+AGG + chr4_1:61836898-61836917 Msa0562200:CDS 55.0%
! CTGGGTTGGGTTGTTCGCCA+TGG + chr4_1:61833879-61833898 Msa0562200:exon 60.0%
ATCTGTGGCGTGGGGACAGA+TGG - chr4_1:61835321-61835340 None:intergenic 60.0%
TGTCCCCACGCCACAGATTG+CGG + chr4_1:61835323-61835342 Msa0562200:CDS 60.0%
TTGCCGCAATCTGTGGCGTG+GGG - chr4_1:61835329-61835348 None:intergenic 60.0%
GTTGCCGCAATCTGTGGCGT+GGG - chr4_1:61835330-61835349 None:intergenic 60.0%
TGTTGCCGCAATCTGTGGCG+TGG - chr4_1:61835331-61835350 None:intergenic 60.0%
ATGTACCGCCACCACCTGCA+GGG + chr4_1:61835676-61835695 Msa0562200:CDS 60.0%
! TCTAACCCTGCAGGTGGTGG+CGG - chr4_1:61835684-61835703 None:intergenic 60.0%
! TTAGGCCCATTGGTTGGCGG+TGG - chr4_1:61835741-61835760 None:intergenic 60.0%
CGGGCATTAGGCCCATTGGT+TGG - chr4_1:61835747-61835766 None:intergenic 60.0%
AGACCGGGCATTAGGCCCAT+TGG - chr4_1:61835751-61835770 None:intergenic 60.0%
GAACCGGCAGTCACCTAGAG+AGG + chr4_1:61836375-61836394 Msa0562200:CDS 60.0%
GTCCCTCTCTAGGTGACTGC+CGG - chr4_1:61836381-61836400 None:intergenic 60.0%
GTGGCCGGTTGTCATCTGGA+AGG + chr4_1:61836420-61836439 Msa0562200:CDS 60.0%
GCCTCATGAGGGAAGGATCC+AGG + chr4_1:61836451-61836470 Msa0562200:CDS 60.0%
CCAGCAGCAGCAGCAGTTGC+CGG - chr4_1:61835297-61835316 None:intergenic 65.0%
CATGTACCGCCACCACCTGC+AGG + chr4_1:61835675-61835694 Msa0562200:CDS 65.0%
GCCGTTTCCCTTCCTGCGCT+TGG - chr4_1:61836186-61836205 None:intergenic 65.0%
GCAGCAGCAGCAGTTGCCGG+TGG - chr4_1:61835294-61835313 None:intergenic 70.0%
CCGGCAACTGCTGCTGCTGC+TGG + chr4_1:61835294-61835313 Msa0562200:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 61833617 61837396 61833617 ID=Msa0562200;Name=Msa0562200
chr4_1 mRNA 61833617 61837396 61833617 ID=Msa0562200-mRNA-1;Parent=Msa0562200;Name=Msa0562200-mRNA-1;_AED=0.00;_eAED=0.00;_QI=281|1|1|1|1|1|3|327|679
chr4_1 exon 61833617 61834023 61833617 ID=Msa0562200-mRNA-1:exon:19497;Parent=Msa0562200-mRNA-1
chr4_1 exon 61835023 61835133 61835023 ID=Msa0562200-mRNA-1:exon:19498;Parent=Msa0562200-mRNA-1
chr4_1 exon 61835267 61837396 61835267 ID=Msa0562200-mRNA-1:exon:19499;Parent=Msa0562200-mRNA-1
chr4_1 five_prime_UTR 61833617 61833897 61833617 ID=Msa0562200-mRNA-1:five_prime_utr;Parent=Msa0562200-mRNA-1
chr4_1 CDS 61833898 61834023 61833898 ID=Msa0562200-mRNA-1:cds;Parent=Msa0562200-mRNA-1
chr4_1 CDS 61835023 61835133 61835023 ID=Msa0562200-mRNA-1:cds;Parent=Msa0562200-mRNA-1
chr4_1 CDS 61835267 61837069 61835267 ID=Msa0562200-mRNA-1:cds;Parent=Msa0562200-mRNA-1
chr4_1 three_prime_UTR 61837070 61837396 61837070 ID=Msa0562200-mRNA-1:three_prime_utr;Parent=Msa0562200-mRNA-1
Gene Sequence

>Msa0562200

ATGGTTGCCCAACAGCCACAACTTCAGATTCAGACTCAGCAACCAACACCTTCTCCTCAAGATGAAGCCTTGAAAAGGAACACCGATTGTGTCTACTTTCTTGCTTCTCCCTTGACATGCAAAAAGGGAAATGAATGTGAGTACCGCCATAGTGAGTATGCTAGAGTCAATCCTAGAGATTGTTGGTATTGGTTGAATGGTAACTGCTTGAATCCCAAGTGTTCATTCCGTCATCCGCCTCTTGACGGGTTGTTAGGAACACCACCGGCAACTGCTGCTGCTGCTGGACCATCTGTCCCCACGCCACAGATTGCGGCAACATCTGCAACCCATGCACCGCCTTATAATGGTAGTAAACAAGCCGTGCCTTGCATTTTCTTTCAAAAGGGGTTTTGCTTAAAAGGTGACAGATGTGCCTTCCTGCATGGACCAAACCCTACCAATGGCAGTAAAATAGCTACACAGGGGTCAATGACAATGACCAACCAAGGAGCTGAGAATCCAAGTTTTAAGAAACCTTTTGGCGGCATTGAAAAACATGCACCAGAAAGGAAAACTTCCCAAGCAAATGTTGCAGGTGTTGAAGCTAAACCTGTCCAAAAATTTGAGACTGCTCCACAGAAAAATATGTTTAAGTTGGTGAAGCATGTACCGCCACCACCTGCAGGGTTAGATAATGAGGCTTCGCGATTTAAGACAACTAGTTCTCCACCGCCAACCAATGGGCCTAATGCCCGGTCTAATCGTGTGCATCAACCTCGTTTGCCAGATGATCACAATTTCCATAGTGGTAAGGACAATGACGAGTTTCTCAGAGAGTCGTCTCCTGGGTTTGATGTTCTTGTAGCTGACGAACTTAGAAATTCTGATTACTATCATGGGGAAGATGAATTTGGTAAAACAAGAGGTCAAGATGAAAGAGGCCCAGACTCTTTGAATGAATACGATGTAGGGCATTCTGCTGATTATAGTTTAGCTGCTGATATTGATCGAGATAGATTTCGTGTGCCCCAAGGTTATGACTCATATGATCACATGCAAGAGCCATATGGCTGGGAACCTAGAAAGGGATCAGCCCAAATAGAAAGAAGGACTCATCACAGATCTCGTAGTCCTGACAGCGTTGAGGTCTCAGATCTCCGACATCGTTTATCCAAGCGCAGGAAGGGAAACGGCTTAAAATCTGTCGTCGCTCATGATGAGGAGCAAAGTCATCGATTTTCTCGGAAGGATTCACACCAGTTGCCTGCAAATGAGAGATCCGTCAATAATCGTTTCCGAGGCAGAATAAATCTTCCACCAAATGGTGGTGACAGTCACCTAGAGAGGGACTTAGACACAGGAAGAACCGGCAGTCACCTAGAGAGGGACTTGGACAGAGGAAGAATCAGTGGCCGGTTGTCATCTGGAAGGCTTCAATCGCCTCATGAGGGAAGGATCCAGGATAGATTAAGAGGAAGGTTGCCAGATGACGAGAGGAGAAACTTCAGCAGTCGATCGAATGAGGACCGAAGTGGGTTCTCTGCGCCAAAAAGTCTTGCTGAACTTAAGTATGGGAGGAACACTGAGAATAATGACCAACAATCATTTGGAAAGAGGAAAAGCTTCAGGGATCACCAACAATATGAAGATGATGTACCATTTGAAGGTCCAAAACCACTTAGTGAAATTCTGAAGGAGAAGAAAGGAGTTGGAGCTGGTGCAGGCTCCTCCCACAGTAGCAAACATGATGACAATAAGAATGAAGGAATTACCGAAAACGGAACATTGTCAGAGAGCAAGGAAGAAGAGTCCAAGATTCAGGCTGCTGATGGAGTTGACAATACTGATGTAACTCACGGTCGATCATCTGAGGAAGGAATGATATACGATGAAGCTGCCGAAGATCAGGAGTATGAAGGTGAAGGAGACTATGAATATGAGCAAGGTGATGAGGAGTATGAGTATGAACAAGTTGAGGGTGAAAATCAAGAGCAAGAATATATGGAAGATGAAGATGGGGATGACTTTGCCAAGAAGATTGGTGTTGTTCTTTCATAA
Protein sequence

>Msa0562200

MVAQQPQLQIQTQQPTPSPQDEALKRNTDCVYFLASPLTCKKGNECEYRHSEYARVNPRDCWYWLNGNCLNPKCSFRHPPLDGLLGTPPATAAAAGPSVPTPQIAATSATHAPPYNGSKQAVPCIFFQKGFCLKGDRCAFLHGPNPTNGSKIATQGSMTMTNQGAENPSFKKPFGGIEKHAPERKTSQANVAGVEAKPVQKFETAPQKNMFKLVKHVPPPPAGLDNEASRFKTTSSPPPTNGPNARSNRVHQPRLPDDHNFHSGKDNDEFLRESSPGFDVLVADELRNSDYYHGEDEFGKTRGQDERGPDSLNEYDVGHSADYSLAADIDRDRFRVPQGYDSYDHMQEPYGWEPRKGSAQIERRTHHRSRSPDSVEVSDLRHRLSKRRKGNGLKSVVAHDEEQSHRFSRKDSHQLPANERSVNNRFRGRINLPPNGGDSHLERDLDTGRTGSHLERDLDRGRISGRLSSGRLQSPHEGRIQDRLRGRLPDDERRNFSSRSNEDRSGFSAPKSLAELKYGRNTENNDQQSFGKRKSFRDHQQYEDDVPFEGPKPLSEILKEKKGVGAGAGSSHSSKHDDNKNEGITENGTLSESKEEESKIQAADGVDNTDVTHGRSSEEGMIYDEAAEDQEYEGEGDYEYEQGDEEYEYEQVEGENQEQEYMEDEDGDDFAKKIGVVLS*