AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0563600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563600 MtrunA17_Chr8g0377401 95.652 253 11 0 1 253 1 253 3.12e-159 449
Msa0563600 MtrunA17_Chr8g0377421 60.405 346 75 2 1 330 1 300 6.36e-137 395
Msa0563600 MtrunA17_Chr3g0122411 50.993 453 185 12 1 435 1 434 1.82e-116 347
Msa0563600 MtrunA17_Chr8g0377391 77.976 168 21 1 259 410 1 168 2.41e-81 248
Msa0563600 MtrunA17_Chr8g0377431 55.844 231 59 5 1 215 1 204 1.22e-57 188
Msa0563600 MtrunA17_Chr8g0360361 57.955 88 37 0 159 246 62 149 4.07e-29 112
Msa0563600 MtrunA17_Chr7g0258471 65.789 76 26 0 159 234 139 214 4.28e-28 115
Msa0563600 MtrunA17_Chr8g0377411 63.043 92 32 1 331 422 1 90 6.82e-28 106
Msa0563600 MtrunA17_Chr4g0046991 58.140 86 34 1 150 235 63 146 3.20e-27 106
Msa0563600 MtrunA17_Chr8g0334671 44.521 146 70 4 94 234 120 259 5.42e-27 110
Msa0563600 MtrunA17_Chr7g0231911 45.802 131 51 2 116 234 99 221 2.23e-26 108
Msa0563600 MtrunA17_Chr5g0420051 58.667 75 31 0 160 234 179 253 9.26e-25 105
Msa0563600 MtrunA17_Chr4g0061651 39.490 157 71 5 99 234 42 195 4.83e-24 103
Msa0563600 MtrunA17_Chr2g0282521 57.333 75 32 0 160 234 62 136 6.74e-24 97.1
Msa0563600 MtrunA17_Chr7g0224821 51.765 85 41 0 150 234 65 149 3.47e-23 100
Msa0563600 MtrunA17_Chr3g0132091 45.872 109 57 1 162 270 73 179 8.15e-23 99.0
Msa0563600 MtrunA17_Chr2g0308321 52.273 88 41 1 147 234 157 243 1.38e-22 100
Msa0563600 MtrunA17_Chr8g0386271 44.737 114 60 1 162 275 99 209 2.42e-21 95.5
Msa0563600 MtrunA17_Chr1g0162871 45.872 109 51 2 127 234 75 176 2.88e-21 97.1
Msa0563600 MtrunA17_Chr2g0302791 46.218 119 56 2 127 241 104 218 4.06e-21 96.7
Msa0563600 MtrunA17_Chr1g0175031 47.115 104 37 2 123 208 17 120 4.82e-21 88.6
Msa0563600 MtrunA17_Chr1g0194121 56.757 74 32 0 161 234 146 219 1.01e-20 95.1
Msa0563600 MtrunA17_Chr2g0286661 34.483 174 97 5 162 329 103 265 2.57e-20 94.0
Msa0563600 MtrunA17_Chr7g0270541 40.426 141 75 3 146 286 129 260 3.81e-20 93.6
Msa0563600 MtrunA17_Chr2g0317041 50.769 65 31 1 144 208 82 145 1.76e-14 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0563600 AT1G27360.2 43.137 255 116 8 1 234 1 247 1.18e-40 149
Msa0563600 AT1G27360.1 43.137 255 116 8 1 234 1 247 1.18e-40 149
Msa0563600 AT1G27360.3 43.137 255 116 8 1 234 1 247 1.18e-40 149
Msa0563600 AT1G27360.4 43.137 255 116 8 1 234 1 247 1.18e-40 149
Msa0563600 AT5G43270.3 34.562 434 195 20 1 393 1 386 4.32e-37 140
Msa0563600 AT5G43270.1 34.562 434 195 20 1 393 1 386 4.32e-37 140
Msa0563600 AT5G43270.2 34.562 434 195 20 1 393 1 386 4.32e-37 140
Msa0563600 AT1G27370.6 41.633 245 114 7 12 234 11 248 1.26e-36 137
Msa0563600 AT1G27370.1 41.633 245 114 7 12 234 11 248 3.70e-36 137
Msa0563600 AT1G27370.5 41.633 245 114 7 12 234 11 248 3.70e-36 137
Msa0563600 AT1G27370.3 41.633 245 114 7 12 234 11 248 3.70e-36 137
Msa0563600 AT1G27370.2 41.633 245 114 7 12 234 11 248 3.70e-36 137
Msa0563600 AT1G27370.4 41.633 245 114 7 12 234 11 248 3.70e-36 137
Msa0563600 AT1G27370.7 41.633 245 114 7 12 234 11 248 3.70e-36 137
Msa0563600 AT2G42200.1 45.517 145 56 4 160 304 72 193 1.07e-28 116
Msa0563600 AT3G57920.1 45.312 128 53 3 129 243 27 150 6.77e-27 110
Msa0563600 AT1G69170.1 62.069 87 30 2 150 234 111 196 8.31e-27 111
Msa0563600 AT1G02065.1 49.123 114 52 1 127 234 147 260 1.40e-26 109
Msa0563600 AT2G33810.1 58.667 75 31 0 161 235 53 127 9.66e-25 99.4
Msa0563600 AT3G15270.1 56.757 74 32 0 161 234 62 135 3.25e-23 96.7
Msa0563600 AT1G53160.2 58.108 74 31 0 161 234 53 126 2.71e-22 94.0
Msa0563600 AT1G53160.1 58.108 74 31 0 161 234 53 126 2.71e-22 94.0
Msa0563600 AT1G53160.3 58.108 74 31 0 161 234 53 126 3.01e-22 93.6
Msa0563600 AT5G50570.1 54.667 75 34 0 160 234 99 173 4.55e-22 97.4
Msa0563600 AT5G50570.2 54.667 75 34 0 160 234 99 173 4.55e-22 97.4
Msa0563600 AT5G50670.1 54.667 75 34 0 160 234 99 173 4.55e-22 97.4
Msa0563600 AT5G50670.2 54.667 75 34 0 160 234 99 173 4.55e-22 97.4
Msa0563600 AT3G60030.1 57.534 73 31 0 162 234 127 199 3.11e-21 97.1
Msa0563600 AT2G47070.1 50.538 93 39 1 142 234 93 178 1.46e-20 95.1
Msa0563600 AT1G20980.1 56.000 75 33 0 160 234 118 192 5.15e-20 93.6
Msa0563600 AT1G76580.2 56.000 75 33 0 160 234 112 186 2.06e-19 91.3
Msa0563600 AT1G76580.3 56.000 75 33 0 160 234 112 186 2.44e-19 91.3
Msa0563600 AT1G76580.1 56.000 75 33 0 160 234 112 186 3.95e-19 90.9
Msa0563600 AT1G02065.2 46.067 89 42 1 127 209 147 235 8.24e-17 80.1
Msa0563600 AT5G18830.3 46.575 73 39 0 162 234 138 210 6.90e-16 80.5
Msa0563600 AT5G18830.1 46.575 73 39 0 162 234 138 210 6.95e-16 80.5
Msa0563600 AT5G18830.2 46.575 73 39 0 162 234 138 210 7.59e-16 80.5
Msa0563600 AT2G47070.2 60.417 48 19 0 187 234 2 49 1.90e-11 66.6

Find 77 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCTGTTTGAGTTTGA+TGG 0.225842 4_1:-64041413 Msa0563600:CDS
CATTGATCCTCTTGGTTTCA+AGG 0.239743 4_1:+64040300 None:intergenic
CAAGGACTTTGGATGTAAAA+TGG 0.260990 4_1:+64040318 None:intergenic
TCAATCTAAAACTTGGTAAA+AGG 0.269090 4_1:-64041860 Msa0563600:CDS
ACTTTCAAATGCAAATTTAC+TGG 0.299044 4_1:+64041983 None:intergenic
TATAACTACCATAGGAGATT+TGG 0.321384 4_1:+64041677 None:intergenic
GCTTCAATAGGCCCTTCTTT+AGG 0.325945 4_1:+64041927 None:intergenic
CCTCTTGGTTTCAAGGACTT+TGG 0.327846 4_1:+64040307 None:intergenic
AGCTTTACCTGCTACATTGC+TGG 0.356873 4_1:+64041631 None:intergenic
ACAAAGATGATTGACTTGAT+TGG 0.359832 4_1:+64041318 None:intergenic
CTCTAGCCTCATCAGAATAT+TGG 0.364549 4_1:-64039876 Msa0563600:CDS
GTGCCAATGTTGTCGTTTAT+AGG 0.365991 4_1:+64040380 None:intergenic
TATGGTAGTTATAGATGGTT+TGG 0.373519 4_1:-64041667 Msa0563600:CDS
GACATTCATGTTAGTCCAAC+TGG 0.375986 4_1:+64039737 None:intergenic
GCAGTCTTTGAATAAGCAAA+TGG 0.377299 4_1:+64040470 None:intergenic
CAAAGATGATTGACTTGATT+GGG 0.382950 4_1:+64041319 None:intergenic
CCGCCTCTCTGCCAGGTTGA+AGG 0.385535 4_1:-64041765 Msa0563600:CDS
TGCTATGGCTTGAGCGGTAC+TGG 0.386033 4_1:+64039921 None:intergenic
TCTCCTATGGTAGTTATAGA+TGG 0.387501 4_1:-64041672 Msa0563600:CDS
CTGACCTGCATTGATCCTCT+TGG 0.395116 4_1:+64040292 None:intergenic
CTCTGCCAGGTTGAAGGTTG+TGG 0.395224 4_1:-64041759 Msa0563600:CDS
CTCCCTCTTCACAGTGGGAA+TGG 0.400387 4_1:-64042220 Msa0563600:CDS
CCAACTGGAAGTGGTTATTC+TGG 0.417567 4_1:-64042089 Msa0563600:CDS
CATCAAACTCAAACAGATCA+TGG 0.444519 4_1:+64041414 None:intergenic
TCCCTCTTCACAGTGGGAAT+GGG 0.446844 4_1:-64042219 Msa0563600:CDS
GGTGCATTAGAAGCTTCAAT+AGG 0.450824 4_1:+64041915 None:intergenic
ATGTTCTAGAGAAACGGATT+TGG 0.460049 4_1:+64039957 None:intergenic
AAAATTGAATTACCTAAAGA+AGG 0.460103 4_1:-64041939 Msa0563600:CDS
TGGTTGTTCGATACGCCAAC+TGG 0.470221 4_1:-64042104 Msa0563600:CDS
ATCTGAATGTTCTAGAGAAA+CGG 0.471048 4_1:+64039951 None:intergenic
CAATCAGTTCATCACCAACT+AGG 0.472722 4_1:-64042019 Msa0563600:CDS
TTCGATACGCCAACTGGAAG+TGG 0.476010 4_1:-64042098 Msa0563600:CDS
CCATTGCTCAATCTAAAACT+TGG 0.477854 4_1:-64041867 Msa0563600:CDS
TCTAATTCGATACTCCAGTT+TGG 0.479917 4_1:+64042143 None:intergenic
ACATTGTAAGTTCTGAGCAT+TGG 0.495722 4_1:+64041788 None:intergenic
ACAATGTCCGCCTCTCTGCC+AGG 0.504313 4_1:-64041772 Msa0563600:CDS
TGACAGAAGAGAGGAAGCAC+AGG 0.512878 4_1:+64040005 None:intergenic
GACGTGTCATCTTTAGTTGT+TGG 0.515414 4_1:-64041825 Msa0563600:CDS
CAGTCTTTGAATAAGCAAAT+GGG 0.517662 4_1:+64040471 None:intergenic
GATAGCATAGTTACAGTACT+TGG 0.519974 4_1:+64040356 None:intergenic
CTCTTCTGTCAACCAGTCCG+TGG 0.520942 4_1:-64039993 Msa0563600:CDS
TTTACCATTGGAATCATCAT+GGG 0.526394 4_1:+64041959 None:intergenic
AGATCATTCCAAATCTCCTA+TGG 0.535644 4_1:-64041685 Msa0563600:CDS
TGTCATCTTTAGTTGTTGGA+AGG 0.541340 4_1:-64041821 Msa0563600:CDS
TTGTGGCCTTGACCTTTCAT+CGG 0.541799 4_1:-64041742 Msa0563600:CDS
CTGCCTCGTTGTGTGTGCTA+TGG 0.544802 4_1:+64039906 None:intergenic
CGCATATGAATCCCACGGAC+TGG 0.545835 4_1:+64039981 None:intergenic
TAGACCGAGAAAATAATGTC+TGG 0.549417 4_1:-64042124 Msa0563600:CDS
AATCAGTTCATCACCAACTA+GGG 0.553568 4_1:-64042018 Msa0563600:CDS
AAACCATCTATAACTACCAT+AGG 0.563385 4_1:+64041669 None:intergenic
TCCCATTCCCACTGTGAAGA+GGG 0.564064 4_1:+64042218 None:intergenic
ACTGGTGAAAACACGACTCA+AGG 0.568330 4_1:+64039755 None:intergenic
TGAGTTTGAGATCCACATGC+TGG 0.569545 4_1:+64039825 None:intergenic
TCTTCTGTCAACCAGTCCGT+GGG 0.576912 4_1:-64039992 Msa0563600:CDS
CAAAATCTCCCTCTTCACAG+TGG 0.588317 4_1:-64042226 Msa0563600:CDS
AAATTGAATTACCTAAAGAA+GGG 0.590524 4_1:-64041938 Msa0563600:CDS
TGGTAAAAGGATGAAATTCG+AGG 0.590856 4_1:-64041847 Msa0563600:CDS
CAAGGCCACAACCTTCAACC+TGG 0.591428 4_1:+64041754 None:intergenic
ATCCCATTCCCACTGTGAAG+AGG 0.600244 4_1:+64042217 None:intergenic
CTAGATACACAAGAAAGCAT+TGG 0.604314 4_1:+64041894 None:intergenic
AAAATCTCCCTCTTCACAGT+GGG 0.605421 4_1:-64042225 Msa0563600:CDS
AAGCCTATAAACGACAACAT+TGG 0.614145 4_1:-64040383 Msa0563600:CDS
GAAACCAAGAGGATCAATGC+AGG 0.614595 4_1:-64040296 Msa0563600:intron
AGTTCCCATGATGATTCCAA+TGG 0.616986 4_1:-64041963 Msa0563600:CDS
CCAGAATAACCACTTCCAGT+TGG 0.626070 4_1:+64042089 None:intergenic
CAATATTCTGATGAGGCTAG+AGG 0.629859 4_1:+64039877 None:intergenic
AATATGTCGATTCCAGCATG+TGG 0.630965 4_1:-64039837 Msa0563600:CDS
CAACCTTCAACCTGGCAGAG+AGG 0.635133 4_1:+64041762 None:intergenic
CCAAAGTCCTTGAAACCAAG+AGG 0.636381 4_1:-64040307 Msa0563600:CDS
TTTGGATGTAAAATGGACAT+CGG 0.653646 4_1:+64040325 None:intergenic
CGGACTGGTTGACAGAAGAG+AGG 0.658311 4_1:+64039996 None:intergenic
ACTGTGCCAATATTCTGATG+AGG 0.658353 4_1:+64039870 None:intergenic
CCTTCAACCTGGCAGAGAGG+CGG 0.660903 4_1:+64041765 None:intergenic
TCTTTGTCTTCGTCACCCTG+TGG 0.667989 4_1:-64041302 Msa0563600:intron
TTGGTCGCATATGAATCCCA+CGG 0.682290 4_1:+64039976 None:intergenic
TGTGTGTGCTATGGCTTGAG+CGG 0.704952 4_1:+64039915 None:intergenic
AAGCCATAGCACACACAACG+AGG 0.721368 4_1:-64039909 Msa0563600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAACATAATCTATTTATTT+TGG - chr4_1:64041784-64041803 Msa0563600:CDS 10.0%
!!! AGGAAATAATAGTATTATTT+TGG - chr4_1:64040416-64040435 Msa0563600:CDS 15.0%
!! AAACATAAATGAACTAAAAT+TGG + chr4_1:64041387-64041406 None:intergenic 15.0%
!! AAAATTGAATTACCTAAAGA+AGG - chr4_1:64040047-64040066 Msa0563600:CDS 20.0%
!! AAATTGAATTACCTAAAGAA+GGG - chr4_1:64040048-64040067 Msa0563600:CDS 20.0%
!!! CAGTTTTACAATAATGATTA+CGG + chr4_1:64040647-64040666 None:intergenic 20.0%
!!! CTCATTTTTCATTTATTTCA+TGG - chr4_1:64041015-64041034 Msa0563600:intron 20.0%
!!! ATTTATTTCATGGATCTTTT+TGG - chr4_1:64041025-64041044 Msa0563600:intron 20.0%
!!! AGTTGAATTTTTATTACATG+AGG + chr4_1:64041329-64041348 None:intergenic 20.0%
!! TGTAATTGATTAGAGTAATA+TGG - chr4_1:64041844-64041863 Msa0563600:CDS 20.0%
!! ATTTTCAGTAGCTTTTGTAT+CGG + chr4_1:64039808-64039827 None:intergenic 25.0%
! AAAAATTACAACTACCAAAC+TGG - chr4_1:64039829-64039848 Msa0563600:CDS 25.0%
!!! TAATTTTTGGTTCTACATCA+GGG - chr4_1:64039928-64039947 Msa0563600:CDS 25.0%
! ACTTTCAAATGCAAATTTAC+TGG + chr4_1:64040006-64040025 None:intergenic 25.0%
!! AGGTAATTCAATTTTACCAT+TGG + chr4_1:64040042-64040061 None:intergenic 25.0%
!! TCAATCTAAAACTTGGTAAA+AGG - chr4_1:64040126-64040145 Msa0563600:intron 25.0%
!!! ATTAAACTTGCTGTCTTTTT+TGG - chr4_1:64040493-64040512 Msa0563600:CDS 25.0%
! GAAAAATAAGTATTACCACA+GGG + chr4_1:64040702-64040721 None:intergenic 25.0%
! AGAAAAATAAGTATTACCAC+AGG + chr4_1:64040703-64040722 None:intergenic 25.0%
! AATCAAATAACAAGCAGAAA+CGG + chr4_1:64040745-64040764 None:intergenic 25.0%
!!! TTCTGCTTGTTATTTGATTT+AGG - chr4_1:64040746-64040765 Msa0563600:intron 25.0%
! GATTAAGCATCTTTATCTTT+AGG + chr4_1:64041070-64041089 None:intergenic 25.0%
!!! TGTATAGTGGATTTACTTAA+AGG - chr4_1:64041212-64041231 Msa0563600:intron 25.0%
! GATTAGAAGATAAAAGAGAA+TGG + chr4_1:64041460-64041479 None:intergenic 25.0%
!!! AAGAGAAAATCTTTTGTTTG+GGG + chr4_1:64041582-64041601 None:intergenic 25.0%
!!! CAAGAGAAAATCTTTTGTTT+GGG + chr4_1:64041583-64041602 None:intergenic 25.0%
!! TCAAGAGAAAATCTTTTGTT+TGG + chr4_1:64041584-64041603 None:intergenic 25.0%
! AAACTTAGTAGAAAATCATG+AGG + chr4_1:64041744-64041763 None:intergenic 25.0%
! TTTTCAGTAGCTTTTGTATC+GGG + chr4_1:64039807-64039826 None:intergenic 30.0%
! ACAACCAGACATTATTTTCT+CGG + chr4_1:64039869-64039888 None:intergenic 30.0%
!!! CTAATTTTTGGTTCTACATC+AGG - chr4_1:64039927-64039946 Msa0563600:CDS 30.0%
TTTACCATTGGAATCATCAT+GGG + chr4_1:64040030-64040049 None:intergenic 30.0%
! TTTTACCATTGGAATCATCA+TGG + chr4_1:64040031-64040050 None:intergenic 30.0%
! AATAATGTTTTGCCGATGAA+AGG + chr4_1:64040259-64040278 None:intergenic 30.0%
TATAACTACCATAGGAGATT+TGG + chr4_1:64040312-64040331 None:intergenic 30.0%
AAACCATCTATAACTACCAT+AGG + chr4_1:64040320-64040339 None:intergenic 30.0%
TATGGTAGTTATAGATGGTT+TGG - chr4_1:64040319-64040338 Msa0563600:CDS 30.0%
TTAACAACGGTATGATTGTT+TGG - chr4_1:64040380-64040399 Msa0563600:CDS 30.0%
TGTTTGGATCTAATTGACAT+AGG - chr4_1:64040396-64040415 Msa0563600:CDS 30.0%
!! CAAAGATGATTGACTTGATT+GGG + chr4_1:64040670-64040689 None:intergenic 30.0%
!! ACAAAGATGATTGACTTGAT+TGG + chr4_1:64040671-64040690 None:intergenic 30.0%
!! TTGATTTAGGTTGTTAAACC+TGG - chr4_1:64040759-64040778 Msa0563600:intron 30.0%
!!! GACTTCTAGATTTTTTTTCC+AGG + chr4_1:64040780-64040799 None:intergenic 30.0%
TTCCTGCTTACTAACTTTAT+AGG - chr4_1:64040899-64040918 Msa0563600:intron 30.0%
TACCTATAAAGTTAGTAAGC+AGG + chr4_1:64040904-64040923 None:intergenic 30.0%
TTATCTTATTCGTGTCACAA+AGG + chr4_1:64041092-64041111 None:intergenic 30.0%
ATAATAACCATGCAGAGTAA+TGG - chr4_1:64041145-64041164 Msa0563600:intron 30.0%
TCATATTAGAGTTGCTAGAA+GGG - chr4_1:64041168-64041187 Msa0563600:intron 30.0%
AAACAGTTATCGTGATTGTT+TGG - chr4_1:64041242-64041261 Msa0563600:intron 30.0%
TGATGTTCCTAAAACTAGAT+TGG - chr4_1:64041280-64041299 Msa0563600:intron 30.0%
AACTAAAATTGGCTACTCTT+CGG + chr4_1:64041376-64041395 None:intergenic 30.0%
CAGTCTTTGAATAAGCAAAT+GGG + chr4_1:64041518-64041537 None:intergenic 30.0%
!! AGAGAAAATCTTTTGTTTGG+GGG + chr4_1:64041581-64041600 None:intergenic 30.0%
TTTGGATGTAAAATGGACAT+CGG + chr4_1:64041664-64041683 None:intergenic 30.0%
TTACTCTAATCAATTACACC+TGG + chr4_1:64041842-64041861 None:intergenic 30.0%
AAATGAATAACACTGTGCAA+GGG + chr4_1:64041891-64041910 None:intergenic 30.0%
!! ATCTGAATGTTCTAGAGAAA+CGG + chr4_1:64042038-64042057 None:intergenic 30.0%
TCTAATTCGATACTCCAGTT+TGG + chr4_1:64039846-64039865 None:intergenic 35.0%
TAGACCGAGAAAATAATGTC+TGG - chr4_1:64039862-64039881 Msa0563600:CDS 35.0%
AATCAGTTCATCACCAACTA+GGG - chr4_1:64039968-64039987 Msa0563600:CDS 35.0%
! GTTTTGCTAATATCCCTAGT+TGG + chr4_1:64039984-64040003 None:intergenic 35.0%
! CTAGATACACAAGAAAGCAT+TGG + chr4_1:64040095-64040114 None:intergenic 35.0%
!!! CCAAGTTTTAGATTGAGCAA+TGG + chr4_1:64040122-64040141 None:intergenic 35.0%
! CCATTGCTCAATCTAAAACT+TGG - chr4_1:64040119-64040138 Msa0563600:intron 35.0%
TGGTAAAAGGATGAAATTCG+AGG - chr4_1:64040139-64040158 Msa0563600:intron 35.0%
TGTCATCTTTAGTTGTTGGA+AGG - chr4_1:64040165-64040184 Msa0563600:intron 35.0%
!! ACATTGTAAGTTCTGAGCAT+TGG + chr4_1:64040201-64040220 None:intergenic 35.0%
AGATCATTCCAAATCTCCTA+TGG - chr4_1:64040301-64040320 Msa0563600:CDS 35.0%
TCTCCTATGGTAGTTATAGA+TGG - chr4_1:64040314-64040333 Msa0563600:CDS 35.0%
CAGGTAAAGCTTCTTAACAA+CGG - chr4_1:64040367-64040386 Msa0563600:CDS 35.0%
!!! AACTTGCTGTCTTTTTTGGA+TGG - chr4_1:64040497-64040516 Msa0563600:intron 35.0%
CACTTTGCTTAAAATGCTGA+AGG - chr4_1:64040547-64040566 Msa0563600:intron 35.0%
CATCAAACTCAAACAGATCA+TGG + chr4_1:64040575-64040594 None:intergenic 35.0%
! CATGATCTGTTTGAGTTTGA+TGG - chr4_1:64040573-64040592 Msa0563600:intron 35.0%
AAGCAGAAACGGATATAAAC+TGG + chr4_1:64040734-64040753 None:intergenic 35.0%
! AGTGACTATTGTAACTTGCA+AGG + chr4_1:64040928-64040947 None:intergenic 35.0%
AATATGACCATTACTCTGCA+TGG + chr4_1:64041155-64041174 None:intergenic 35.0%
GTCATATTAGAGTTGCTAGA+AGG - chr4_1:64041167-64041186 Msa0563600:intron 35.0%
!!! ACTCACACCAATCTAGTTTT+AGG + chr4_1:64041290-64041309 None:intergenic 35.0%
GCAGTCTTTGAATAAGCAAA+TGG + chr4_1:64041519-64041538 None:intergenic 35.0%
!! GCTGTTTTGTATGTTTTGCA+AGG + chr4_1:64041553-64041572 None:intergenic 35.0%
AAGCCTATAAACGACAACAT+TGG - chr4_1:64041603-64041622 Msa0563600:intron 35.0%
GATAGCATAGTTACAGTACT+TGG + chr4_1:64041633-64041652 None:intergenic 35.0%
CAAGGACTTTGGATGTAAAA+TGG + chr4_1:64041671-64041690 None:intergenic 35.0%
CAAATGAATAACACTGTGCA+AGG + chr4_1:64041892-64041911 None:intergenic 35.0%
! CTTGCACAGTGTTATTCATT+TGG - chr4_1:64041890-64041909 Msa0563600:CDS 35.0%
! TTTGGTAGAATACCTTTTGC+AGG - chr4_1:64041908-64041927 Msa0563600:CDS 35.0%
!! ATGTTCTAGAGAAACGGATT+TGG + chr4_1:64042032-64042051 None:intergenic 35.0%
AAAATCTCCCTCTTCACAGT+GGG - chr4_1:64039761-64039780 Msa0563600:CDS 40.0%
!! CTTTTGTATCGGGTAATGGT+AGG + chr4_1:64039797-64039816 None:intergenic 40.0%
! GTAGCTTTTGTATCGGGTAA+TGG + chr4_1:64039801-64039820 None:intergenic 40.0%
!!! TCTGGCTCTGAGCTAATTTT+TGG - chr4_1:64039915-64039934 Msa0563600:CDS 40.0%
CAATCAGTTCATCACCAACT+AGG - chr4_1:64039967-64039986 Msa0563600:CDS 40.0%
AGTTCCCATGATGATTCCAA+TGG - chr4_1:64040023-64040042 Msa0563600:CDS 40.0%
GGTGCATTAGAAGCTTCAAT+AGG + chr4_1:64040074-64040093 None:intergenic 40.0%
GACGTGTCATCTTTAGTTGT+TGG - chr4_1:64040161-64040180 Msa0563600:intron 40.0%
! AGGGATTGCTATTAGAGTTG+TGG - chr4_1:64041187-64041206 Msa0563600:intron 40.0%
! GGGATTGCTATTAGAGTTGT+GGG - chr4_1:64041188-64041207 Msa0563600:intron 40.0%
! GTGCCAATGTTGTCGTTTAT+AGG + chr4_1:64041609-64041628 None:intergenic 40.0%
CATTGATCCTCTTGGTTTCA+AGG + chr4_1:64041689-64041708 None:intergenic 40.0%
CATGGTGGTAGCAATCAATA+TGG + chr4_1:64041810-64041829 None:intergenic 40.0%
TACACCTGGCAATATTTGCA+TGG + chr4_1:64041828-64041847 None:intergenic 40.0%
! AATACCTTTTGCAGGTGTAG+AGG - chr4_1:64041916-64041935 Msa0563600:CDS 40.0%
AAATCCTCTACACCTGCAAA+AGG + chr4_1:64041923-64041942 None:intergenic 40.0%
CAATATTCTGATGAGGCTAG+AGG + chr4_1:64042112-64042131 None:intergenic 40.0%
CTCTAGCCTCATCAGAATAT+TGG - chr4_1:64042110-64042129 Msa0563600:CDS 40.0%
ACTGTGCCAATATTCTGATG+AGG + chr4_1:64042119-64042138 None:intergenic 40.0%
!! AATATGTCGATTCCAGCATG+TGG - chr4_1:64042149-64042168 Msa0563600:CDS 40.0%
CAAAATCTCCCTCTTCACAG+TGG - chr4_1:64039760-64039779 Msa0563600:CDS 45.0%
CCAGAATAACCACTTCCAGT+TGG + chr4_1:64039900-64039919 None:intergenic 45.0%
CCAACTGGAAGTGGTTATTC+TGG - chr4_1:64039897-64039916 Msa0563600:CDS 45.0%
GCTTCAATAGGCCCTTCTTT+AGG + chr4_1:64040062-64040081 None:intergenic 45.0%
! TTGTGGCCTTGACCTTTCAT+CGG - chr4_1:64040244-64040263 Msa0563600:intron 45.0%
! GTTTTGCCGATGAAAGGTCA+AGG + chr4_1:64040253-64040272 None:intergenic 45.0%
! GATTTTGCCAGCAATGTAGC+AGG - chr4_1:64040348-64040367 Msa0563600:CDS 45.0%
AGCTTTACCTGCTACATTGC+TGG + chr4_1:64040358-64040377 None:intergenic 45.0%
! AGTCTAAGACCATGCTATGC+AGG - chr4_1:64040796-64040815 Msa0563600:intron 45.0%
CAACTATAGCCTGCATAGCA+TGG + chr4_1:64040808-64040827 None:intergenic 45.0%
! TAGAGTTGTGGGCTGTATAG+TGG - chr4_1:64041199-64041218 Msa0563600:intron 45.0%
CCTCTTGGTTTCAAGGACTT+TGG + chr4_1:64041682-64041701 None:intergenic 45.0%
CCAAAGTCCTTGAAACCAAG+AGG - chr4_1:64041679-64041698 Msa0563600:CDS 45.0%
GAAACCAAGAGGATCAATGC+AGG - chr4_1:64041690-64041709 Msa0563600:CDS 45.0%
ACCACCATGCAAATATTGCC+AGG - chr4_1:64041821-64041840 Msa0563600:CDS 45.0%
ACCTGGCAATATTTGCATGG+TGG + chr4_1:64041825-64041844 None:intergenic 45.0%
TTGGTCGCATATGAATCCCA+CGG + chr4_1:64042013-64042032 None:intergenic 45.0%
! TGAGTTTGAGATCCACATGC+TGG + chr4_1:64042164-64042183 None:intergenic 45.0%
ACTGGTGAAAACACGACTCA+AGG + chr4_1:64042234-64042253 None:intergenic 45.0%
! TGAGTCGTGTTTTCACCAGT+TGG - chr4_1:64042234-64042253 Msa0563600:CDS 45.0%
TCCCTCTTCACAGTGGGAAT+GGG - chr4_1:64039767-64039786 Msa0563600:CDS 50.0%
TCCCATTCCCACTGTGAAGA+GGG + chr4_1:64039771-64039790 None:intergenic 50.0%
ATCCCATTCCCACTGTGAAG+AGG + chr4_1:64039772-64039791 None:intergenic 50.0%
TGGTTGTTCGATACGCCAAC+TGG - chr4_1:64039882-64039901 Msa0563600:CDS 50.0%
TTCGATACGCCAACTGGAAG+TGG - chr4_1:64039888-64039907 Msa0563600:CDS 50.0%
TCTTTGTCTTCGTCACCCTG+TGG - chr4_1:64040684-64040703 Msa0563600:intron 50.0%
! CTGACCTGCATTGATCCTCT+TGG + chr4_1:64041697-64041716 None:intergenic 50.0%
! TGACAGAAGAGAGGAAGCAC+AGG + chr4_1:64041984-64042003 None:intergenic 50.0%
TCTTCTGTCAACCAGTCCGT+GGG - chr4_1:64041994-64042013 Msa0563600:CDS 50.0%
TGTGTGTGCTATGGCTTGAG+CGG + chr4_1:64042074-64042093 None:intergenic 50.0%
AAGCCATAGCACACACAACG+AGG - chr4_1:64042077-64042096 Msa0563600:CDS 50.0%
CTCCCTCTTCACAGTGGGAA+TGG - chr4_1:64039766-64039785 Msa0563600:CDS 55.0%
CAACCTTCAACCTGGCAGAG+AGG + chr4_1:64040227-64040246 None:intergenic 55.0%
! CTCTGCCAGGTTGAAGGTTG+TGG - chr4_1:64040227-64040246 Msa0563600:intron 55.0%
CAAGGCCACAACCTTCAACC+TGG + chr4_1:64040235-64040254 None:intergenic 55.0%
!! CGGACTGGTTGACAGAAGAG+AGG + chr4_1:64041993-64042012 None:intergenic 55.0%
CTCTTCTGTCAACCAGTCCG+TGG - chr4_1:64041993-64042012 Msa0563600:CDS 55.0%
CGCATATGAATCCCACGGAC+TGG + chr4_1:64042008-64042027 None:intergenic 55.0%
TGCTATGGCTTGAGCGGTAC+TGG + chr4_1:64042068-64042087 None:intergenic 55.0%
CTGCCTCGTTGTGTGTGCTA+TGG + chr4_1:64042083-64042102 None:intergenic 55.0%
ACAATGTCCGCCTCTCTGCC+AGG - chr4_1:64040214-64040233 Msa0563600:intron 60.0%
CCTTCAACCTGGCAGAGAGG+CGG + chr4_1:64040224-64040243 None:intergenic 60.0%
! CCGCCTCTCTGCCAGGTTGA+AGG - chr4_1:64040221-64040240 Msa0563600:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 64039747 64042261 64039747 ID=Msa0563600;Name=Msa0563600
chr4_1 mRNA 64039747 64042261 64039747 ID=Msa0563600-mRNA-1;Parent=Msa0563600;Name=Msa0563600-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|1|1|4|0|435
chr4_1 exon 64041639 64042261 64041639 ID=Msa0563600-mRNA-1:exon:20424;Parent=Msa0563600-mRNA-1
chr4_1 exon 64041303 64041439 64041303 ID=Msa0563600-mRNA-1:exon:20423;Parent=Msa0563600-mRNA-1
chr4_1 exon 64040297 64040512 64040297 ID=Msa0563600-mRNA-1:exon:20422;Parent=Msa0563600-mRNA-1
chr4_1 exon 64039747 64040078 64039747 ID=Msa0563600-mRNA-1:exon:20421;Parent=Msa0563600-mRNA-1
chr4_1 CDS 64041639 64042261 64041639 ID=Msa0563600-mRNA-1:cds;Parent=Msa0563600-mRNA-1
chr4_1 CDS 64041303 64041439 64041303 ID=Msa0563600-mRNA-1:cds;Parent=Msa0563600-mRNA-1
chr4_1 CDS 64040297 64040512 64040297 ID=Msa0563600-mRNA-1:cds;Parent=Msa0563600-mRNA-1
chr4_1 CDS 64039747 64040078 64039747 ID=Msa0563600-mRNA-1:cds;Parent=Msa0563600-mRNA-1
Gene Sequence

>Msa0563600

ATGGATTTGGATGCAAAATCTCCCTCTTCACAGTGGGAATGGGATCACCTACCATTACCCGATACAAAAGCTACTGAAAATAAAAAATTACAACTACCAAACTGGAGTATCGAATTAGACCGAGAAAATAATGTCTGGTTGTTCGATACGCCAACTGGAAGTGGTTATTCTGGCTCTGAGCTAATTTTTGGTTCTACATCAGGGAGTTCGAAATCAGATTCAATCAGTTCATCACCAACTAGGGATATTAGCAAAACCAGTAAATTTGCATTTGAAAGTTCCCATGATGATTCCAATGGTAAAATTGAATTACCTAAAGAAGGGCCTATTGAAGCTTCTAATGCACCAATGCTTTCTTGTGTATCTAGTGAACCATTGCTCAATCTAAAACTTGGTAAAAGGATGAAATTCGAGGACGTGTCATCTTTAGTTGTTGGAAGGAAATGTAAGTCCAATGCTCAGAACTTACAATGTCCGCCTCTCTGCCAGGTTGAAGGTTGTGGCCTTGACCTTTCATCGGCAAAACATTATTATCGCAAACGAAGAGTTTGTGTAGATCATTCCAAATCTCCTATGGTAGTTATAGATGGTTTGGAACGACGATTTTGCCAGCAATGTAGCAGGTTCCATGATCTGTTTGAGTTTGATGGAAAGAAGAAAAGCTGCAGACGACGTCTTTCACAACACAATGCATTGCGCCGTAATCATTATTGTAAAACTGCCCAATCAAGTCAATCATCTTTGTCTTCGTCACCCTGTGATGAGAAGCAACAGATGAACCCATTTGCTTATTCAAAGACTGCTATAAATTTAGCCTTGCAAAACATACAAAACAGCGAGCTCCCCCAAACAAAAGATTTTCTCTTGAAGCCTATAAACGACAACATTGGCACACCAAGTACTGTAACTATGCTATCTGATGATTCCGATGTCCATTTTACATCCAAAGTCCTTGAAACCAAGAGGATCAATGCAGGTGTAGAGGATTTCATTAGCTTCTCTGATACAGATGCTACACAAGACTTCACCTGTGCTTCCTCTCTTCTGTCAACCAGTCCGTGGGATTCATATGCGACCAAATCCGTTTCTCTAGAACATTCAGATTGTACAACCAGTACCGCTCAAGCCATAGCACACACAACGAGGCAGTGTATGCCTCTAGCCTCATCAGAATATTGGCACAGTGTTCTTGATCAATATGTCGATTCCAGCATGTGGATCTCAAACTCAGACTGTGAAGAAAGCGATCATTTTCAAGAATTTCAATGTTCTGAGAACCTTGAGTCGTGTTTTCACCAGTTGGACTAA
Protein sequence

>Msa0563600

MDLDAKSPSSQWEWDHLPLPDTKATENKKLQLPNWSIELDRENNVWLFDTPTGSGYSGSELIFGSTSGSSKSDSISSSPTRDISKTSKFAFESSHDDSNGKIELPKEGPIEASNAPMLSCVSSEPLLNLKLGKRMKFEDVSSLVVGRKCKSNAQNLQCPPLCQVEGCGLDLSSAKHYYRKRRVCVDHSKSPMVVIDGLERRFCQQCSRFHDLFEFDGKKKSCRRRLSQHNALRRNHYCKTAQSSQSSLSSSPCDEKQQMNPFAYSKTAINLALQNIQNSELPQTKDFLLKPINDNIGTPSTVTMLSDDSDVHFTSKVLETKRINAGVEDFISFSDTDATQDFTCASSLLSTSPWDSYATKSVSLEHSDCTTSTAQAIAHTTRQCMPLASSEYWHSVLDQYVDSSMWISNSDCEESDHFQEFQCSENLESCFHQLD*