Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563920 | KEH20638.1 | 66.197 | 213 | 61 | 2 | 3 | 206 | 179 | 389 | 2.57e-88 | 275 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563920 | A0A072TUV6 | 66.197 | 213 | 61 | 2 | 3 | 206 | 179 | 389 | 1.23e-88 | 275 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0563920 | TR | mTERF |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563920 | MtrunA17_Chr8g0377801 | 66.197 | 213 | 61 | 2 | 3 | 206 | 179 | 389 | 3.16e-92 | 275 |
| Msa0563920 | MtrunA17_Chr8g0377781 | 67.476 | 206 | 60 | 2 | 4 | 202 | 167 | 372 | 4.75e-87 | 261 |
| Msa0563920 | MtrunA17_Chr2g0296191 | 33.117 | 154 | 103 | 0 | 53 | 206 | 359 | 512 | 1.94e-19 | 85.9 |
| Msa0563920 | MtrunA17_Chr2g0296191 | 34.228 | 149 | 98 | 0 | 53 | 201 | 846 | 994 | 7.47e-18 | 81.3 |
| Msa0563920 | MtrunA17_Chr2g0295731 | 30.323 | 155 | 108 | 0 | 51 | 205 | 224 | 378 | 2.99e-19 | 84.7 |
| Msa0563920 | MtrunA17_Chr2g0296211 | 32.051 | 156 | 106 | 0 | 49 | 204 | 203 | 358 | 5.27e-19 | 84.0 |
| Msa0563920 | MtrunA17_Chr2g0296251 | 33.553 | 152 | 101 | 0 | 53 | 204 | 247 | 398 | 1.20e-18 | 82.8 |
| Msa0563920 | MtrunA17_Chr2g0296701 | 32.432 | 148 | 100 | 0 | 57 | 204 | 9 | 156 | 1.30e-18 | 79.3 |
| Msa0563920 | MtrunA17_Chr2g0296551 | 33.784 | 148 | 98 | 0 | 57 | 204 | 37 | 184 | 3.43e-18 | 79.0 |
| Msa0563920 | MtrunA17_Chr2g0315731 | 31.361 | 169 | 115 | 1 | 37 | 204 | 235 | 403 | 5.59e-18 | 81.3 |
| Msa0563920 | MtrunA17_Chr2g0296711 | 30.061 | 163 | 114 | 0 | 42 | 204 | 224 | 386 | 1.04e-17 | 80.5 |
| Msa0563920 | MtrunA17_Chr2g0296741 | 30.921 | 152 | 105 | 0 | 53 | 204 | 252 | 403 | 2.33e-17 | 79.3 |
| Msa0563920 | MtrunA17_Chr2g0296171 | 32.484 | 157 | 103 | 1 | 53 | 206 | 244 | 400 | 5.83e-17 | 78.2 |
| Msa0563920 | MtrunA17_Chr2g0296221 | 32.026 | 153 | 104 | 0 | 53 | 205 | 263 | 415 | 6.43e-17 | 78.2 |
| Msa0563920 | MtrunA17_Chr2g0296131 | 30.065 | 153 | 107 | 0 | 51 | 203 | 9 | 161 | 2.44e-16 | 73.6 |
| Msa0563920 | MtrunA17_Chr2g0296751 | 31.783 | 129 | 88 | 0 | 76 | 204 | 1 | 129 | 4.52e-16 | 72.8 |
| Msa0563920 | MtrunA17_Chr2g0296151 | 29.697 | 165 | 116 | 0 | 40 | 204 | 206 | 370 | 7.51e-16 | 75.1 |
| Msa0563920 | MtrunA17_Chr2g0296721 | 30.263 | 152 | 106 | 0 | 53 | 204 | 231 | 382 | 8.48e-16 | 75.1 |
| Msa0563920 | MtrunA17_Chr2g0286221 | 28.402 | 169 | 117 | 3 | 37 | 204 | 4 | 169 | 9.98e-16 | 72.0 |
| Msa0563920 | MtrunA17_Chr2g0296121 | 28.659 | 164 | 117 | 0 | 41 | 204 | 293 | 456 | 1.02e-15 | 74.7 |
| Msa0563920 | MtrunA17_Chr2g0296291 | 32.237 | 152 | 103 | 0 | 53 | 204 | 247 | 398 | 1.59e-15 | 74.3 |
| Msa0563920 | MtrunA17_Chr4g0067121 | 29.221 | 154 | 109 | 0 | 51 | 204 | 227 | 380 | 2.10e-15 | 73.6 |
| Msa0563920 | MtrunA17_Chr2g0295761 | 28.947 | 152 | 107 | 1 | 54 | 204 | 238 | 389 | 1.36e-13 | 68.6 |
| Msa0563920 | MtrunA17_Chr4g0067141 | 28.481 | 158 | 113 | 0 | 45 | 202 | 213 | 370 | 1.84e-13 | 68.2 |
| Msa0563920 | MtrunA17_Chr2g0295741 | 26.316 | 171 | 123 | 1 | 34 | 204 | 209 | 376 | 1.92e-13 | 68.2 |
| Msa0563920 | MtrunA17_Chr2g0286191 | 29.032 | 155 | 106 | 3 | 51 | 204 | 208 | 359 | 1.92e-13 | 68.2 |
| Msa0563920 | MtrunA17_Chr2g0296161 | 27.027 | 148 | 108 | 0 | 57 | 204 | 11 | 158 | 4.31e-13 | 64.7 |
| Msa0563920 | MtrunA17_Chr4g0001831 | 29.032 | 155 | 104 | 2 | 51 | 202 | 217 | 368 | 8.38e-13 | 66.2 |
| Msa0563920 | MtrunA17_Chr8g0339041 | 30.667 | 150 | 99 | 3 | 56 | 202 | 201 | 348 | 1.06e-12 | 65.9 |
| Msa0563920 | MtrunA17_Chr2g0296301 | 25.166 | 151 | 113 | 0 | 53 | 203 | 215 | 365 | 8.41e-12 | 63.2 |
| Msa0563920 | MtrunA17_Chr2g0296111 | 29.870 | 154 | 107 | 1 | 51 | 204 | 218 | 370 | 1.47e-11 | 62.8 |
| Msa0563920 | MtrunA17_Chr2g0296231 | 28.834 | 163 | 114 | 1 | 42 | 204 | 220 | 380 | 1.74e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0563920 | AT5G64950.1 | 36.866 | 217 | 122 | 4 | 5 | 206 | 164 | 380 | 1.44e-34 | 126 |
| Msa0563920 | AT1G21150.3 | 28.977 | 176 | 115 | 3 | 26 | 198 | 179 | 347 | 4.00e-18 | 81.6 |
| Msa0563920 | AT1G21150.1 | 28.977 | 176 | 115 | 3 | 26 | 198 | 215 | 383 | 5.33e-18 | 81.6 |
| Msa0563920 | AT1G21150.2 | 28.977 | 176 | 115 | 3 | 26 | 198 | 279 | 447 | 6.77e-18 | 81.6 |
| Msa0563920 | AT5G07900.1 | 30.000 | 150 | 104 | 1 | 53 | 201 | 250 | 399 | 4.24e-17 | 79.0 |
| Msa0563920 | AT1G61990.2 | 28.729 | 181 | 91 | 3 | 56 | 198 | 225 | 405 | 8.32e-16 | 75.5 |
| Msa0563920 | AT1G61990.1 | 28.729 | 181 | 91 | 3 | 56 | 198 | 225 | 405 | 8.32e-16 | 75.5 |
| Msa0563920 | AT1G62010.1 | 26.471 | 170 | 89 | 3 | 56 | 189 | 216 | 385 | 2.27e-11 | 62.4 |
| Msa0563920 | AT1G61970.2 | 32.727 | 110 | 70 | 2 | 82 | 187 | 290 | 399 | 2.80e-11 | 62.4 |
| Msa0563920 | AT1G61970.7 | 32.727 | 110 | 70 | 2 | 82 | 187 | 290 | 399 | 2.80e-11 | 62.4 |
| Msa0563920 | AT1G61970.6 | 32.727 | 110 | 70 | 2 | 82 | 187 | 290 | 399 | 2.80e-11 | 62.4 |
| Msa0563920 | AT1G61970.3 | 32.727 | 110 | 70 | 2 | 82 | 187 | 290 | 399 | 2.80e-11 | 62.4 |
| Msa0563920 | AT1G61970.5 | 32.727 | 110 | 70 | 2 | 82 | 187 | 290 | 399 | 2.80e-11 | 62.4 |
| Msa0563920 | AT1G61970.4 | 32.727 | 110 | 70 | 2 | 82 | 187 | 290 | 399 | 2.80e-11 | 62.4 |
| Msa0563920 | AT1G61970.1 | 32.727 | 110 | 70 | 2 | 82 | 187 | 290 | 399 | 2.80e-11 | 62.4 |
Find 28 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGATTTCATTCAGCATTT+TGG | 0.077878 | 4_1:-64376821 | Msa0563920:CDS |
| TCTGATACCCTAAGCAAATT+TGG | 0.167535 | 4_1:+64376758 | None:intergenic |
| GTTTGAGCCATAAAACATTT+AGG | 0.239728 | 4_1:-64376850 | Msa0563920:CDS |
| AACAATGCCACAACTCTCTA+AGG | 0.240848 | 4_1:+64377018 | None:intergenic |
| GAGCTTGCAAATGTTCAAAA+AGG | 0.301950 | 4_1:-64376783 | Msa0563920:CDS |
| TAGAGAGTTGTGGCATTGTT+GGG | 0.304274 | 4_1:-64377015 | Msa0563920:CDS |
| TTTATCTGAGGACACGTTCT+TGG | 0.315238 | 4_1:-64376546 | Msa0563920:CDS |
| TTGTGGCATTGTTGGGGATC+AGG | 0.331357 | 4_1:-64377008 | Msa0563920:CDS |
| AAAAGGTTCCAAATTTGCTT+AGG | 0.354562 | 4_1:-64376766 | Msa0563920:CDS |
| GGTATCAGAGAAGAAGATAA+AGG | 0.405797 | 4_1:-64376744 | Msa0563920:CDS |
| CAAAGTCAGCATTGGTTACT+CGG | 0.409355 | 4_1:-64376685 | Msa0563920:CDS |
| TTAGAGAGTTGTGGCATTGT+TGG | 0.418272 | 4_1:-64377016 | Msa0563920:CDS |
| CTCTAAGGGCAACAAATGTT+TGG | 0.420557 | 4_1:+64377033 | None:intergenic |
| AAAGGTTCCAAATTTGCTTA+GGG | 0.440166 | 4_1:-64376765 | Msa0563920:CDS |
| TTTAACACATAAACAAAGCT+TGG | 0.454268 | 4_1:+64376569 | None:intergenic |
| CGAGTAACCAATGCTGACTT+TGG | 0.457799 | 4_1:+64376686 | None:intergenic |
| TCAGAGAAGAAGATAAAGGT+TGG | 0.472055 | 4_1:-64376740 | Msa0563920:CDS |
| ATCACAAAATCTATGTAAGA+AGG | 0.510006 | 4_1:-64376594 | Msa0563920:CDS |
| GGCATTATTGATAGCTTACA+AGG | 0.510418 | 4_1:-64376489 | Msa0563920:CDS |
| AGAGAGTTGTGGCATTGTTG+GGG | 0.519144 | 4_1:-64377014 | Msa0563920:CDS |
| GCAACAAATGTTTGGTACAC+TGG | 0.546385 | 4_1:+64377041 | None:intergenic |
| TATGCTGCCAAAGTCAGCAT+TGG | 0.579046 | 4_1:-64376693 | Msa0563920:CDS |
| TTGTTGCCCTTAGAGAGTTG+TGG | 0.581308 | 4_1:-64377025 | Msa0563920:CDS |
| TTATGTGTTAAATTTATCTG+AGG | 0.602383 | 4_1:-64376558 | Msa0563920:CDS |
| ACAATGCCACAACTCTCTAA+GGG | 0.616141 | 4_1:+64377019 | None:intergenic |
| TAGTTTCTAATACGAGATTG+TGG | 0.627705 | 4_1:+64376947 | None:intergenic |
| ATGCTATACATCAAAATCAG+AGG | 0.630674 | 4_1:+64376662 | None:intergenic |
| AAAGTTTAAAGAAAATGCAG+AGG | 0.668498 | 4_1:-64376510 | Msa0563920:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATGTGTTAAATTTATCTG+AGG | - | chr4_1:64376997-64377016 | Msa0563920:CDS | 20.0% |
| !!! | GCTTTTTCCTAAATGTTTTA+TGG | + | chr4_1:64376715-64376734 | None:intergenic | 25.0% |
| !! | TTGATTTCATTCAGCATTTT+GGG | - | chr4_1:64376735-64376754 | Msa0563920:CDS | 25.0% |
| ! | ATCACAAAATCTATGTAAGA+AGG | - | chr4_1:64376961-64376980 | Msa0563920:CDS | 25.0% |
| ! | TTTAACACATAAACAAAGCT+TGG | + | chr4_1:64376989-64377008 | None:intergenic | 25.0% |
| !! | TCTGCATTTTCTTTAAACTT+TGG | + | chr4_1:64377046-64377065 | None:intergenic | 25.0% |
| ! | AAAGTTTAAAGAAAATGCAG+AGG | - | chr4_1:64377045-64377064 | Msa0563920:CDS | 25.0% |
| TAGTTTCTAATACGAGATTG+TGG | + | chr4_1:64376611-64376630 | None:intergenic | 30.0% | |
| ! | TGTTTTGCAAGCTGAAAAAT+TGG | - | chr4_1:64376631-64376650 | Msa0563920:CDS | 30.0% |
| ! | GTTTTGCAAGCTGAAAAATT+GGG | - | chr4_1:64376632-64376651 | Msa0563920:CDS | 30.0% |
| ! | CAAGCATTCTTGAATTTTGA+TGG | + | chr4_1:64376661-64376680 | None:intergenic | 30.0% |
| GTTTGAGCCATAAAACATTT+AGG | - | chr4_1:64376705-64376724 | Msa0563920:CDS | 30.0% | |
| ! | CTTGATTTCATTCAGCATTT+TGG | - | chr4_1:64376734-64376753 | Msa0563920:CDS | 30.0% |
| ! | AAAAGGTTCCAAATTTGCTT+AGG | - | chr4_1:64376789-64376808 | Msa0563920:CDS | 30.0% |
| ! | AAAGGTTCCAAATTTGCTTA+GGG | - | chr4_1:64376790-64376809 | Msa0563920:CDS | 30.0% |
| ATGCTATACATCAAAATCAG+AGG | + | chr4_1:64376896-64376915 | None:intergenic | 30.0% | |
| !! | TCTGATTTTGATGTATAGCA+TGG | - | chr4_1:64376895-64376914 | Msa0563920:CDS | 30.0% |
| ATCTGAAAAACTCTAAATCG+AGG | + | chr4_1:64376935-64376954 | None:intergenic | 30.0% | |
| !!! | AATCAAGTTTTGAGCTACTG+TGG | - | chr4_1:64376481-64376500 | Msa0563920:CDS | 35.0% |
| GAGCTTGCAAATGTTCAAAA+AGG | - | chr4_1:64376772-64376791 | Msa0563920:CDS | 35.0% | |
| TCTGATACCCTAAGCAAATT+TGG | + | chr4_1:64376800-64376819 | None:intergenic | 35.0% | |
| GGTATCAGAGAAGAAGATAA+AGG | - | chr4_1:64376811-64376830 | Msa0563920:CDS | 35.0% | |
| TCAGAGAAGAAGATAAAGGT+TGG | - | chr4_1:64376815-64376834 | Msa0563920:CDS | 35.0% | |
| !! | GATTTTGATGTATAGCATGG+AGG | - | chr4_1:64376898-64376917 | Msa0563920:CDS | 35.0% |
| ! | GGCATTATTGATAGCTTACA+AGG | - | chr4_1:64377066-64377085 | Msa0563920:CDS | 35.0% |
| GCAACAAATGTTTGGTACAC+TGG | + | chr4_1:64376517-64376536 | None:intergenic | 40.0% | |
| ! | CTCTAAGGGCAACAAATGTT+TGG | + | chr4_1:64376525-64376544 | None:intergenic | 40.0% |
| ACAATGCCACAACTCTCTAA+GGG | + | chr4_1:64376539-64376558 | None:intergenic | 40.0% | |
| AACAATGCCACAACTCTCTA+AGG | + | chr4_1:64376540-64376559 | None:intergenic | 40.0% | |
| TTAGAGAGTTGTGGCATTGT+TGG | - | chr4_1:64376539-64376558 | Msa0563920:CDS | 40.0% | |
| TAGAGAGTTGTGGCATTGTT+GGG | - | chr4_1:64376540-64376559 | Msa0563920:CDS | 40.0% | |
| !! | CAAAGTCAGCATTGGTTACT+CGG | - | chr4_1:64376870-64376889 | Msa0563920:CDS | 40.0% |
| TTTATCTGAGGACACGTTCT+TGG | - | chr4_1:64377009-64377028 | Msa0563920:CDS | 40.0% | |
| !!! | AAGTTTTGAGCTACTGTGGC+TGG | - | chr4_1:64376485-64376504 | Msa0563920:CDS | 45.0% |
| TTGTTGCCCTTAGAGAGTTG+TGG | - | chr4_1:64376530-64376549 | Msa0563920:CDS | 45.0% | |
| AGAGAGTTGTGGCATTGTTG+GGG | - | chr4_1:64376541-64376560 | Msa0563920:CDS | 45.0% | |
| TATGCTGCCAAAGTCAGCAT+TGG | - | chr4_1:64376862-64376881 | Msa0563920:CDS | 45.0% | |
| !! | CGAGTAACCAATGCTGACTT+TGG | + | chr4_1:64376872-64376891 | None:intergenic | 45.0% |
| TTGTGGCATTGTTGGGGATC+AGG | - | chr4_1:64376547-64376566 | Msa0563920:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 64376475 | 64377102 | 64376475 | ID=Msa0563920;Name=Msa0563920 |
| chr4_1 | mRNA | 64376475 | 64377102 | 64376475 | ID=Msa0563920-mRNA-1;Parent=Msa0563920;Name=Msa0563920-mRNA-1;_AED=0.42;_eAED=0.43;_QI=0|0|0|1|0|0|2|0|206 |
| chr4_1 | exon | 64376960 | 64377102 | 64376960 | ID=Msa0563920-mRNA-1:exon:20607;Parent=Msa0563920-mRNA-1 |
| chr4_1 | exon | 64376475 | 64376952 | 64376475 | ID=Msa0563920-mRNA-1:exon:20606;Parent=Msa0563920-mRNA-1 |
| chr4_1 | CDS | 64376960 | 64377102 | 64376960 | ID=Msa0563920-mRNA-1:cds;Parent=Msa0563920-mRNA-1 |
| chr4_1 | CDS | 64376475 | 64376952 | 64376475 | ID=Msa0563920-mRNA-1:cds;Parent=Msa0563920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
