AlfalfaGEDB | Msa0566370

M. sativa cultivar zhongmu-4 / Msa0566370

KEGG: -
GO: -
PFAM: PF00320.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0566370	AET04474.1	85.093	161	19	1	1	161	1	156	1.52e-87	263

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0566370	G7L9K0	85.093	161	19	1	1	161	1	156	7.26e-88	263

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TFs/TRs:

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Gene ID	Type	Classification
Msa0566370	TF	C2C2-GATA

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 30 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACTTGTTACTAACAG+TGG	0.608058	4_1:+67432670	Msa0566370:CDS
ACCGGCTGGTTTAAGAAGAA+CGG	0.594026	4_1:-67437020	None:intergenic
AGATGAGTCATCCTCAGAAG+AGG	0.600054	4_1:+67436940	Msa0566370:CDS
AGCAACTCCAGCATGGCGCC+AGG	0.436034	4_1:+67432552	Msa0566370:CDS
ATCCTCAAACAACGTCTCTT+TGG	0.317203	4_1:-67436842	None:intergenic
ATCTGAATCAGAAACATCAA+TGG	0.539843	4_1:-67432731	None:intergenic
CAATCAGAAGATGACACCCA+TGG	0.614905	4_1:+67436868	Msa0566370:CDS
CATAAAACTGGCTTGTCTTT+TGG	0.254968	4_1:-67432577	None:intergenic
CATCACCAGCAACTCCAGCA+TGG	0.651498	4_1:+67432545	Msa0566370:CDS
CCAAGACCAATTTCATTTGA+AGG	0.412288	4_1:-67436995	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCTGAATCAGAAACATCAA+TGG	-	chr4_1:67432734-67432753	None:intergenic	30.0%
CAATAATGAATTAGTCCAAG+TGG	+	chr4_1:67433026-67433045	Msa0566370:intron	30.0%
AATCCAAAATTCCATCGTTT+AGG	-	chr4_1:67433426-67433445	None:intergenic	30.0%
TGGACTAAGATACCAAAACT+GGG	+	chr4_1:67432609-67432628	Msa0566370:CDS	35.0%
GAAAACAACGTTTGAGAGAA+TGG	-	chr4_1:67432648-67432667	None:intergenic	35.0%
TTAAAATCCGGAATGCACAA+GGG	-	chr4_1:67433136-67433155	None:intergenic	35.0%
ATGAATGTGGAAGTGACATA+TGG	+	chr4_1:67434632-67434651	Msa0566370:intron	35.0%
TCATAGGGACTAAAAGTTGA+AGG	+	chr4_1:67436676-67436695	Msa0566370:intron	35.0%
CCAAGACCAATTTCATTTGA+AGG	-	chr4_1:67436998-67437017	None:intergenic	35.0%
CCACATGCATTGCATAAAAC+TGG	-	chr4_1:67432592-67432611	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	67432538	67437054	67432538	ID=Msa0566370;Name=Msa0566370
chr4_1	mRNA	67432538	67437054	67432538	ID=Msa0566370-mRNA-1;Parent=Msa0566370;Name=Msa0566370-mRNA-1;_AED=0.28;_eAED=0.27;_QI=0\|0\|0\|1\|1\|1\|2\|0\|161
chr4_1	exon	67432538	67432757	67432538	ID=Msa0566370-mRNA-1:exon:22125;Parent=Msa0566370-mRNA-1
chr4_1	exon	67436789	67437054	67436789	ID=Msa0566370-mRNA-1:exon:22126;Parent=Msa0566370-mRNA-1
chr4_1	CDS	67432538	67432757	67432538	ID=Msa0566370-mRNA-1:cds;Parent=Msa0566370-mRNA-1
chr4_1	CDS	67436789	67437054	67436789	ID=Msa0566370-mRNA-1:cds;Parent=Msa0566370-mRNA-1

Gene Sequence

>Msa0566370

ATGGATTCATCACCAGCAACTCCAGCATGGCGCCAGGGACCAAAAGACAAGCCAGTTTTATGCAATGCATGTGGACTAAGATACCAAAACTGGGGAAACCTCAAAGACCATTCTCTCAAACGTTGTTTTCCAAAAGAACTTGTTACTAACAGTGGCAACAGTAGCAACAAGAGTCTCAACGAAAGTTTATCAACCATTGATGTTTCTGATTCAGATAATGAGAATTTTTACTTAGATGAAAAAGATTCAGATTCTTGCAAGTCTGCAGTCTCTCCAAAGAGACGTTGTTTGAGGATTATCAATCAGAAGATGACACCCATGGAAAAGTTTCAACAAGAACTTCTCGATGAGTTGAAAAATCACGAGATACCAGATGAGTCATCCTCAGAAGAGGCCTTATTATTCAAAAATGTGAACAACTTTATACCTTCAAATGAAATTGGTCTTGGAGCCGTTCTTCTTAAACCAGCCGGTTCTTCTATGTAG

Protein sequence

>Msa0566370

MDSSPATPAWRQGPKDKPVLCNACGLRYQNWGNLKDHSLKRCFPKELVTNSGNSSNKSLNESLSTIDVSDSDNENFYLDEKDSDSCKSAVSPKRRCLRIINQKMTPMEKFQQELLDELKNHEIPDESSSEEALLFKNVNNFIPSNEIGLGAVLLKPAGSSM*