Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566380 | AET04474.1 | 92.308 | 156 | 12 | 0 | 1 | 156 | 1 | 156 | 1.93e-95 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566380 | G7L9K0 | 92.308 | 156 | 12 | 0 | 1 | 156 | 1 | 156 | 9.24e-96 | 283 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0566380 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATACCTTCAAATGAAAT+TGG | 0.214941 | 4_1:+67443571 | Msa0566380:CDS |
| CATAAAACTGGCTTGTCTTT+TGG | 0.254968 | 4_1:-67443077 | None:intergenic |
| CTTTGAGGTTTCCCCAGTTT+TGG | 0.256311 | 4_1:-67443121 | None:intergenic |
| ATTCTCAAACTACGTCTCTT+TGG | 0.299791 | 4_1:-67443424 | None:intergenic |
| TCAAACAATAAGGCCTCTTC+TGG | 0.301830 | 4_1:-67443535 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.314815 | 4_1:-67443050 | None:intergenic |
| CTGTTCGTAACAAGTTCTTT+TGG | 0.340372 | 4_1:-67443167 | None:intergenic |
| TTTGGTCCCTGGCGCCATGC+TGG | 0.374123 | 4_1:-67443059 | None:intergenic |
| GAAAACAACGTTTGAGAGAA+TGG | 0.387976 | 4_1:-67443145 | None:intergenic |
| GATGTTTCTGACTCAGATAA+TGG | 0.394861 | 4_1:+67443236 | Msa0566380:CDS |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | 4_1:+67443108 | Msa0566380:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | 4_1:-67443089 | None:intergenic |
| CCTTCAAATGAAATTGGCCT+TGG | 0.419265 | 4_1:+67443577 | Msa0566380:CDS |
| CCAAGGCCAATTTCATTTGA+AGG | 0.421742 | 4_1:-67443577 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.436034 | 4_1:+67443052 | Msa0566380:CDS |
| GTTTGAGAGAATGGTCTTTG+AGG | 0.475222 | 4_1:-67443136 | None:intergenic |
| TCTTCTGGAGATGACTCATC+TGG | 0.489997 | 4_1:-67443520 | None:intergenic |
| TCTTGTTGAAACTTCTCCAT+CGG | 0.530728 | 4_1:-67443466 | None:intergenic |
| TTCTACATAGAAGAACCGTC+TGG | 0.563851 | 4_1:-67443616 | None:intergenic |
| ATCTGAGTCAGAAACATCAA+TGG | 0.564933 | 4_1:-67443231 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.585142 | 4_1:+67443053 | Msa0566380:CDS |
| AGATGAGTCATCTCCAGAAG+AGG | 0.604525 | 4_1:+67443522 | Msa0566380:CDS |
| GCGGTTCTTCTTAAACCAGA+CGG | 0.605404 | 4_1:+67443601 | Msa0566380:CDS |
| AAATGAAATTGGCCTTGGAG+CGG | 0.608265 | 4_1:+67443582 | Msa0566380:CDS |
| CAATCAGAAGATGACACCGA+TGG | 0.614119 | 4_1:+67443450 | Msa0566380:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | 4_1:+67443109 | Msa0566380:CDS |
| GTTTAAGAAGAACCGCTCCA+AGG | 0.637380 | 4_1:-67443594 | None:intergenic |
| AAAGAACTTGTTACGAACAG+TGG | 0.644687 | 4_1:+67443170 | Msa0566380:CDS |
| CATCACCAGCAACTCCAGCA+TGG | 0.651498 | 4_1:+67443045 | Msa0566380:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | 4_1:+67443110 | Msa0566380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTAAAGAAAAGAGTGAAAA+TGG | - | chr4_1:67443284-67443303 | None:intergenic | 25.0% |
| !!! | TGATATATACTACTTTTGTG+AGG | + | chr4_1:67443305-67443324 | Msa0566380:intron | 25.0% |
| !!! | GATATATACTACTTTTGTGA+GGG | + | chr4_1:67443306-67443325 | Msa0566380:intron | 25.0% |
| !! | GTTCACATTTTCAAACAATA+AGG | - | chr4_1:67443548-67443567 | None:intergenic | 25.0% |
| ! | TTCATACCTTCAAATGAAAT+TGG | + | chr4_1:67443571-67443590 | Msa0566380:CDS | 25.0% |
| !!! | CATAAAACTGGCTTGTCTTT+TGG | - | chr4_1:67443080-67443099 | None:intergenic | 35.0% |
| TGGACTAAGATACCAAAACT+GGG | + | chr4_1:67443109-67443128 | Msa0566380:CDS | 35.0% | |
| GAAAACAACGTTTGAGAGAA+TGG | - | chr4_1:67443148-67443167 | None:intergenic | 35.0% | |
| ! | CTGTTCGTAACAAGTTCTTT+TGG | - | chr4_1:67443170-67443189 | None:intergenic | 35.0% |
| !! | AAAGAACTTGTTACGAACAG+TGG | + | chr4_1:67443170-67443189 | Msa0566380:CDS | 35.0% |
| ATCTGAGTCAGAAACATCAA+TGG | - | chr4_1:67443234-67443253 | None:intergenic | 35.0% | |
| ! | GATGTTTCTGACTCAGATAA+TGG | + | chr4_1:67443236-67443255 | Msa0566380:CDS | 35.0% |
| !! | ATATACTACTTTTGTGAGGG+TGG | + | chr4_1:67443309-67443328 | Msa0566380:intron | 35.0% |
| !! | TATACTACTTTTGTGAGGGT+GGG | + | chr4_1:67443310-67443329 | Msa0566380:intron | 35.0% |
| ATTCTCAAACTACGTCTCTT+TGG | - | chr4_1:67443427-67443446 | None:intergenic | 35.0% | |
| TCTTGTTGAAACTTCTCCAT+CGG | - | chr4_1:67443469-67443488 | None:intergenic | 35.0% | |
| CCACATGCATTGCATAAAAC+TGG | - | chr4_1:67443092-67443111 | None:intergenic | 40.0% | |
| !! | CCAGTTTTATGCAATGCATG+TGG | + | chr4_1:67443089-67443108 | Msa0566380:CDS | 40.0% |
| ! | GTGGACTAAGATACCAAAAC+TGG | + | chr4_1:67443108-67443127 | Msa0566380:CDS | 40.0% |
| GGACTAAGATACCAAAACTG+GGG | + | chr4_1:67443110-67443129 | Msa0566380:CDS | 40.0% | |
| GTTTGAGAGAATGGTCTTTG+AGG | - | chr4_1:67443139-67443158 | None:intergenic | 40.0% | |
| TCAAACAATAAGGCCTCTTC+TGG | - | chr4_1:67443538-67443557 | None:intergenic | 40.0% | |
| CCAAGGCCAATTTCATTTGA+AGG | - | chr4_1:67443580-67443599 | None:intergenic | 40.0% | |
| ! | CCTTCAAATGAAATTGGCCT+TGG | + | chr4_1:67443577-67443596 | Msa0566380:CDS | 40.0% |
| !! | AAATGAAATTGGCCTTGGAG+CGG | + | chr4_1:67443582-67443601 | Msa0566380:CDS | 40.0% |
| !!! | CTTTGAGGTTTCCCCAGTTT+TGG | - | chr4_1:67443124-67443143 | None:intergenic | 45.0% |
| CAATCAGAAGATGACACCGA+TGG | + | chr4_1:67443450-67443469 | Msa0566380:CDS | 45.0% | |
| ! | TCTTCTGGAGATGACTCATC+TGG | - | chr4_1:67443523-67443542 | None:intergenic | 45.0% |
| AGATGAGTCATCTCCAGAAG+AGG | + | chr4_1:67443522-67443541 | Msa0566380:CDS | 45.0% | |
| GTTTAAGAAGAACCGCTCCA+AGG | - | chr4_1:67443597-67443616 | None:intergenic | 45.0% | |
| GCGGTTCTTCTTAAACCAGA+CGG | + | chr4_1:67443601-67443620 | Msa0566380:CDS | 45.0% | |
| CATCACCAGCAACTCCAGCA+TGG | + | chr4_1:67443045-67443064 | Msa0566380:CDS | 55.0% | |
| !! | CTGGCTTGTCTTTTGGTCCC+TGG | - | chr4_1:67443073-67443092 | None:intergenic | 55.0% |
| TGGCGCCATGCTGGAGTTGC+TGG | - | chr4_1:67443053-67443072 | None:intergenic | 65.0% | |
| AGCAACTCCAGCATGGCGCC+AGG | + | chr4_1:67443052-67443071 | Msa0566380:CDS | 65.0% | |
| GCAACTCCAGCATGGCGCCA+GGG | + | chr4_1:67443053-67443072 | Msa0566380:CDS | 65.0% | |
| TTTGGTCCCTGGCGCCATGC+TGG | - | chr4_1:67443062-67443081 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 67443038 | 67443636 | 67443038 | ID=Msa0566380;Name=Msa0566380 |
| chr4_1 | mRNA | 67443038 | 67443636 | 67443038 | ID=Msa0566380-mRNA-1;Parent=Msa0566380;Name=Msa0566380-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|1|2|0|156 |
| chr4_1 | exon | 67443038 | 67443257 | 67443038 | ID=Msa0566380-mRNA-1:exon:22123;Parent=Msa0566380-mRNA-1 |
| chr4_1 | exon | 67443386 | 67443636 | 67443386 | ID=Msa0566380-mRNA-1:exon:22124;Parent=Msa0566380-mRNA-1 |
| chr4_1 | CDS | 67443038 | 67443257 | 67443038 | ID=Msa0566380-mRNA-1:cds;Parent=Msa0566380-mRNA-1 |
| chr4_1 | CDS | 67443386 | 67443636 | 67443386 | ID=Msa0566380-mRNA-1:cds;Parent=Msa0566380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
