AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0570690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0570690 MtrunA17_Chr4g0043891 92.800 125 2 2 11 135 1 118 2.50e-78 232
Msa0570690 MtrunA17_Chr8g0392991 60.345 116 35 3 16 131 94 198 3.41e-37 130
Msa0570690 MtrunA17_Chr7g0238431 71.429 70 20 0 11 80 1 70 1.16e-31 112
Msa0570690 MtrunA17_Chr5g0436661 39.552 134 71 2 18 141 33 166 3.40e-28 102
Msa0570690 MtrunA17_Chr5g0405351 67.164 67 21 1 14 79 26 92 2.13e-27 103
Msa0570690 MtrunA17_Chr1g0153171 68.750 64 20 0 17 80 89 152 1.07e-26 100
Msa0570690 MtrunA17_Chr3g0125111 84.615 52 8 0 29 80 91 142 1.70e-26 97.4
Msa0570690 MtrunA17_Chr8g0385441 84.615 52 8 0 29 80 93 144 1.74e-26 97.4
Msa0570690 MtrunA17_Chr4g0031341 66.667 63 21 0 18 80 25 87 5.33e-26 98.6
Msa0570690 MtrunA17_Chr7g0223471 46.377 69 37 0 13 81 42 110 1.11e-18 80.9
Msa0570690 MtrunA17_Chr1g0212511 46.341 82 38 1 18 99 85 160 4.80e-18 77.8
Msa0570690 MtrunA17_Chr2g0283141 44.928 69 38 0 13 81 50 118 6.29e-18 79.0
Msa0570690 MtrunA17_Chr3g0144021 42.623 61 35 0 18 78 82 142 1.01e-13 65.5
Msa0570690 MtrunA17_Chr6g0460691 39.474 76 46 0 17 92 31 106 2.57e-13 65.5
Msa0570690 MtrunA17_Chr7g0222981 36.559 93 49 1 18 110 68 150 6.68e-13 62.8
Msa0570690 MtrunA17_Chr7g0254251 45.098 51 28 0 17 67 27 77 4.77e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0570690 AT3G18010.1 54.472 123 44 3 16 135 74 187 4.04e-36 127
Msa0570690 AT2G28610.1 65.714 70 24 0 11 80 1 70 5.52e-30 108
Msa0570690 AT5G59340.1 64.706 68 24 0 16 83 12 79 1.44e-27 103
Msa0570690 AT3G11260.1 48.421 95 39 1 18 102 24 118 9.56e-27 99.0
Msa0570690 AT2G17950.1 60.759 79 30 1 14 91 34 112 2.20e-26 100
Msa0570690 AT1G46480.2 63.768 69 24 1 12 80 85 152 3.89e-26 97.8
Msa0570690 AT2G01500.1 74.545 55 14 0 18 72 61 115 8.62e-26 98.6
Msa0570690 AT1G46480.1 63.768 69 24 1 12 80 85 152 2.02e-25 97.4
Msa0570690 AT5G05770.1 59.375 64 26 0 18 81 29 92 1.64e-23 89.0
Msa0570690 AT2G33880.1 44.286 70 39 0 13 82 50 119 1.69e-17 77.8
Msa0570690 AT2G33880.2 44.286 70 39 0 13 82 50 119 2.42e-17 77.4
Msa0570690 AT5G45980.1 44.776 67 37 0 13 79 50 116 4.15e-16 73.6
Msa0570690 AT4G35550.1 49.180 61 31 0 18 78 99 159 1.16e-15 72.0
Msa0570690 AT1G20700.2 45.000 60 33 0 18 77 33 92 3.74e-14 65.5
Msa0570690 AT1G20700.3 45.000 60 33 0 18 77 95 154 1.31e-13 65.5
Msa0570690 AT1G20700.1 45.000 60 33 0 18 77 95 154 1.45e-13 65.5
Msa0570690 AT3G03660.1 49.057 53 27 0 17 69 32 84 3.20e-13 65.1
Msa0570690 AT3G03660.2 49.057 53 27 0 17 69 32 84 5.09e-13 64.7
Msa0570690 AT3G03660.4 49.057 53 27 0 17 69 32 84 7.81e-13 64.3
Msa0570690 AT3G03660.3 49.057 53 27 0 17 69 32 84 1.02e-12 64.3
Msa0570690 AT5G17810.1 43.396 53 30 0 17 69 20 72 2.19e-11 60.1
Msa0570690 AT5G17810.2 43.396 53 30 0 17 69 20 72 2.19e-11 60.1
Msa0570690 AT5G17810.3 43.396 53 30 0 17 69 20 72 2.22e-11 60.1

Find 29 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTCTCGCCTTGTGATTC+TGG 0.194023 4_1:-73010564 None:intergenic
GCTTCGCAGCTTCGACAATT+TGG 0.206061 4_1:+73010508 Msa0570690:CDS
ATTTCTCTTGTCTTTGTTGA+TGG 0.263889 4_1:+73011261 Msa0570690:CDS
AGAGAAAGAGAAGTACGTTA+TGG 0.359866 4_1:+73010651 Msa0570690:CDS
CGACAATTTGGAAAGATTGA+AGG 0.396848 4_1:+73010520 Msa0570690:CDS
GAGAAAGAGAAGTACGTTAT+GGG 0.401887 4_1:+73010652 Msa0570690:CDS
GAAGCTGCGAAGCTATTTGT+TGG 0.428703 4_1:-73010498 None:intergenic
CTTGCCTATACAATTCTTCA+AGG 0.428836 4_1:-73010447 None:intergenic
TAGTCCTTGAAGAATTGTAT+AGG 0.431151 4_1:+73010443 Msa0570690:CDS
GTTGGACTCCACCTTGAACT+TGG 0.444756 4_1:-73010406 None:intergenic
CTTGAGATGGAGGAAACAAT+TGG 0.464032 4_1:+73010604 Msa0570690:CDS
TGCATCCTCAACCAAGTTCA+AGG 0.472729 4_1:+73010395 Msa0570690:CDS
CTCCACCTTGAACTTGGTTG+AGG 0.502185 4_1:-73010400 None:intergenic
TGTTGGATTTGTTCAGCTGA+TGG 0.503667 4_1:-73010481 None:intergenic
TTGTTCACTGCAGTTTGAAG+TGG 0.506780 4_1:-73011225 None:intergenic
TCAAGGACTAGTAATTGAAC+TGG 0.509163 4_1:-73010430 None:intergenic
GAAGCGTCGTCGCCTTGAGA+TGG 0.530642 4_1:+73010591 Msa0570690:CDS
ACTAGTAATTGAACTGGTGT+TGG 0.537149 4_1:-73010424 None:intergenic
CTTGAAGAATTGTATAGGCA+AGG 0.540070 4_1:+73010448 Msa0570690:CDS
TCAGAAAAGCAAGAAGCTGC+AGG 0.540109 4_1:+73010679 Msa0570690:CDS
CAATTGTTTCCTCCATCTCA+AGG 0.548253 4_1:-73010603 None:intergenic
GCGTCGTCGCCTTGAGATGG+AGG 0.576407 4_1:+73010594 Msa0570690:CDS
TTGAAGAATTGTATAGGCAA+GGG 0.590766 4_1:+73010449 Msa0570690:CDS
ACAGCTCATGCAGCTGAGCA+AGG 0.595931 4_1:+73010358 None:intergenic
AAACTGCAGTGAACAAGCAG+AGG 0.632631 4_1:+73011232 Msa0570690:CDS
AGTTAAAGAGACAAAGAAGT+GGG 0.667079 4_1:+73011199 Msa0570690:CDS
CTATTGGTTCCAGAATCACA+AGG 0.685006 4_1:+73010555 Msa0570690:CDS
GAGTTAAAGAGACAAAGAAG+TGG 0.689037 4_1:+73011198 Msa0570690:CDS
ATCCTCAACCAAGTTCAAGG+TGG 0.762805 4_1:+73010398 Msa0570690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTTTTCTTTCAAATA+TGG - chr4_1:73011025-73011044 None:intergenic 10.0%
!!! AAATTTTTTTCTTTCAAATA+TGG - chr4_1:73011025-73011044 None:intergenic 10.0%
!!! TTGTTGTTTATTCAATTTAA+TGG + chr4_1:73011046-73011065 Msa0570690:intron 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG + chr4_1:73011077-73011096 Msa0570690:intron 15.0%
!!! TTGTTGTTTATTCAATTTAA+TGG + chr4_1:73011046-73011065 Msa0570690:intron 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG + chr4_1:73011077-73011096 Msa0570690:intron 15.0%
!! TCATGAGTGAAATATAATAT+AGG - chr4_1:73010763-73010782 None:intergenic 20.0%
!!! AAAGATAAATAGCTTTTGTA+AGG + chr4_1:73010819-73010838 Msa0570690:intron 20.0%
!! AGAAAAAAAAATGACCAAAT+GGG - chr4_1:73010885-73010904 None:intergenic 20.0%
!! AAGAAAAAAAAATGACCAAA+TGG - chr4_1:73010886-73010905 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG - chr4_1:73010918-73010937 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG - chr4_1:73011001-73011020 None:intergenic 20.0%
!! TCATGAGTGAAATATAATAT+AGG - chr4_1:73010763-73010782 None:intergenic 20.0%
!!! AAAGATAAATAGCTTTTGTA+AGG + chr4_1:73010819-73010838 Msa0570690:intron 20.0%
!! AGAAAAAAAAATGACCAAAT+GGG - chr4_1:73010885-73010904 None:intergenic 20.0%
!! AAGAAAAAAAAATGACCAAA+TGG - chr4_1:73010886-73010905 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG - chr4_1:73010918-73010937 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG - chr4_1:73011001-73011020 None:intergenic 20.0%
! AAGGAAAAAATGTGTTCTAT+TGG + chr4_1:73010539-73010558 Msa0570690:CDS 25.0%
! TACAGCTAGATATTATCTTT+AGG + chr4_1:73010704-73010723 Msa0570690:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG + chr4_1:73010705-73010724 Msa0570690:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG + chr4_1:73010715-73010734 Msa0570690:intron 25.0%
! AAATATCAATTTAGCCCATT+TGG + chr4_1:73010868-73010887 Msa0570690:intron 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG - chr4_1:73010919-73010938 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG + chr4_1:73010916-73010935 Msa0570690:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG + chr4_1:73010970-73010989 Msa0570690:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG + chr4_1:73011146-73011165 Msa0570690:intron 25.0%
! AAGGAAAAAATGTGTTCTAT+TGG + chr4_1:73010539-73010558 Msa0570690:CDS 25.0%
! TACAGCTAGATATTATCTTT+AGG + chr4_1:73010704-73010723 Msa0570690:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG + chr4_1:73010705-73010724 Msa0570690:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG + chr4_1:73010715-73010734 Msa0570690:intron 25.0%
! AAATATCAATTTAGCCCATT+TGG + chr4_1:73010868-73010887 Msa0570690:intron 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG - chr4_1:73010919-73010938 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG + chr4_1:73010916-73010935 Msa0570690:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG + chr4_1:73010970-73010989 Msa0570690:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG + chr4_1:73011146-73011165 Msa0570690:intron 25.0%
TAGTCCTTGAAGAATTGTAT+AGG + chr4_1:73010443-73010462 Msa0570690:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG + chr4_1:73010449-73010468 Msa0570690:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG + chr4_1:73010727-73010746 Msa0570690:intron 30.0%
! AATAGCTTTTGTAAGGATCT+CGG + chr4_1:73010826-73010845 Msa0570690:intron 30.0%
ACACACACTTCAAATTGTAA+GGG - chr4_1:73011116-73011135 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG + chr4_1:73011199-73011218 Msa0570690:CDS 30.0%
TAGTCCTTGAAGAATTGTAT+AGG + chr4_1:73010443-73010462 Msa0570690:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG + chr4_1:73010449-73010468 Msa0570690:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG + chr4_1:73010727-73010746 Msa0570690:intron 30.0%
! AATAGCTTTTGTAAGGATCT+CGG + chr4_1:73010826-73010845 Msa0570690:intron 30.0%
ACACACACTTCAAATTGTAA+GGG - chr4_1:73011116-73011135 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG + chr4_1:73011199-73011218 Msa0570690:CDS 30.0%
ACTAGTAATTGAACTGGTGT+TGG - chr4_1:73010427-73010446 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG - chr4_1:73010433-73010452 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG - chr4_1:73010450-73010469 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG + chr4_1:73010448-73010467 Msa0570690:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG + chr4_1:73010520-73010539 Msa0570690:CDS 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG + chr4_1:73010651-73010670 Msa0570690:CDS 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG + chr4_1:73010652-73010671 Msa0570690:CDS 35.0%
CACACACACTTCAAATTGTA+AGG - chr4_1:73011117-73011136 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG + chr4_1:73011153-73011172 Msa0570690:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG + chr4_1:73011170-73011189 Msa0570690:intron 35.0%
GAGTTAAAGAGACAAAGAAG+TGG + chr4_1:73011198-73011217 Msa0570690:CDS 35.0%
ACTAGTAATTGAACTGGTGT+TGG - chr4_1:73010427-73010446 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG - chr4_1:73010433-73010452 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG - chr4_1:73010450-73010469 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG + chr4_1:73010448-73010467 Msa0570690:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG + chr4_1:73010520-73010539 Msa0570690:CDS 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG + chr4_1:73010651-73010670 Msa0570690:CDS 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG + chr4_1:73010652-73010671 Msa0570690:CDS 35.0%
CACACACACTTCAAATTGTA+AGG - chr4_1:73011117-73011136 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG + chr4_1:73011153-73011172 Msa0570690:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG + chr4_1:73011170-73011189 Msa0570690:intron 35.0%
GAGTTAAAGAGACAAAGAAG+TGG + chr4_1:73011198-73011217 Msa0570690:CDS 35.0%
! TGTTGGATTTGTTCAGCTGA+TGG - chr4_1:73010484-73010503 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG + chr4_1:73010555-73010574 Msa0570690:CDS 40.0%
CAATTGTTTCCTCCATCTCA+AGG - chr4_1:73010606-73010625 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG + chr4_1:73010604-73010623 Msa0570690:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG + chr4_1:73011173-73011192 Msa0570690:intron 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG + chr4_1:73011176-73011195 Msa0570690:intron 40.0%
TTGTTCACTGCAGTTTGAAG+TGG - chr4_1:73011228-73011247 None:intergenic 40.0%
! TGTTGGATTTGTTCAGCTGA+TGG - chr4_1:73010484-73010503 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG + chr4_1:73010555-73010574 Msa0570690:CDS 40.0%
CAATTGTTTCCTCCATCTCA+AGG - chr4_1:73010606-73010625 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG + chr4_1:73010604-73010623 Msa0570690:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG + chr4_1:73011173-73011192 Msa0570690:intron 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG + chr4_1:73011176-73011195 Msa0570690:intron 40.0%
TTGTTCACTGCAGTTTGAAG+TGG - chr4_1:73011228-73011247 None:intergenic 40.0%
TGCATCCTCAACCAAGTTCA+AGG + chr4_1:73010395-73010414 Msa0570690:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG + chr4_1:73010398-73010417 Msa0570690:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG - chr4_1:73010501-73010520 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG + chr4_1:73010679-73010698 Msa0570690:CDS 45.0%
AAACTGCAGTGAACAAGCAG+AGG + chr4_1:73011232-73011251 Msa0570690:CDS 45.0%
TGCATCCTCAACCAAGTTCA+AGG + chr4_1:73010395-73010414 Msa0570690:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG + chr4_1:73010398-73010417 Msa0570690:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG - chr4_1:73010501-73010520 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG + chr4_1:73010679-73010698 Msa0570690:CDS 45.0%
AAACTGCAGTGAACAAGCAG+AGG + chr4_1:73011232-73011251 Msa0570690:CDS 45.0%
CTCCACCTTGAACTTGGTTG+AGG - chr4_1:73010403-73010422 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG - chr4_1:73010409-73010428 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG + chr4_1:73010508-73010527 Msa0570690:CDS 50.0%
CTCCACCTTGAACTTGGTTG+AGG - chr4_1:73010403-73010422 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG - chr4_1:73010409-73010428 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG + chr4_1:73010508-73010527 Msa0570690:CDS 50.0%
GCTCTCTCGCCTTGTGATTC+TGG - chr4_1:73010567-73010586 None:intergenic 55.0%
GCTCTCTCGCCTTGTGATTC+TGG - chr4_1:73010567-73010586 None:intergenic 55.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG + chr4_1:73010591-73010610 Msa0570690:CDS 60.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG + chr4_1:73010591-73010610 Msa0570690:CDS 60.0%
GCGTCGTCGCCTTGAGATGG+AGG + chr4_1:73010594-73010613 Msa0570690:CDS 65.0%
GCGTCGTCGCCTTGAGATGG+AGG + chr4_1:73010594-73010613 Msa0570690:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 73010364 73011280 73010364 ID=Msa0570690;Name=Msa0570690
chr4_1 mRNA 73010364 73011280 73010364 ID=Msa0570690-mRNA-1;Parent=Msa0570690;Name=Msa0570690-mRNA-1;_AED=0.15;_eAED=0.18;_QI=0|0|0|1|1|1|2|0|143
chr4_1 exon 73010364 73010700 73010364 ID=Msa0570690-mRNA-1:exon:24999;Parent=Msa0570690-mRNA-1
chr4_1 exon 73011186 73011280 73011186 ID=Msa0570690-mRNA-1:exon:25000;Parent=Msa0570690-mRNA-1
chr4_1 CDS 73010364 73010700 73010364 ID=Msa0570690-mRNA-1:cds;Parent=Msa0570690-mRNA-1
chr4_1 CDS 73011186 73011280 73011186 ID=Msa0570690-mRNA-1:cds;Parent=Msa0570690-mRNA-1
Gene Sequence

>Msa0570690

CATGCAGCTGAGCAAGGTACAAGCAATACTATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAGGTCTGTAATTTCTCTTGTCTTTGTTGA

Protein sequence

>Msa0570690

HAAEQGTSNTMHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRRRLEMEETIGSSSTEDKKEKEKYVMGNSEKQEAAETGGGVKETKKWATTSNCSEQAEVCNFSCLC*