AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0575140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575140 MtrunA17_Chr8g0392151 97.510 241 6 0 1 241 1 241 1.50e-179 492
Msa0575140 MtrunA17_Chr1g0161601 46.809 94 49 1 14 107 49 141 4.19e-25 96.7
Msa0575140 MtrunA17_Chr4g0065041 33.793 145 74 3 15 158 10 133 4.89e-21 88.6
Msa0575140 MtrunA17_Chr2g0297251 32.738 168 88 5 15 181 39 182 1.61e-20 87.4
Msa0575140 MtrunA17_Chr7g0230511 30.345 145 78 4 15 158 11 133 4.19e-20 85.9
Msa0575140 MtrunA17_Chr1g0179781 35.374 147 71 6 15 158 9 134 4.10e-19 82.4
Msa0575140 MtrunA17_Chr5g0411151 35.616 146 71 5 15 158 37 161 2.06e-17 78.6
Msa0575140 MtrunA17_Chr7g0265291 31.792 173 92 6 15 182 9 160 3.25e-17 77.4
Msa0575140 MtrunA17_Chr4g0040071 34.932 146 72 5 15 158 17 141 5.87e-17 77.0
Msa0575140 MtrunA17_Chr8g0366581 33.951 162 82 6 1 158 19 159 1.46e-16 76.3
Msa0575140 MtrunA17_Chr5g0395181 32.877 146 75 5 15 158 23 147 7.53e-16 73.9
Msa0575140 MtrunA17_Chr2g0320551 32.215 149 84 5 15 159 6 141 6.27e-15 70.5
Msa0575140 MtrunA17_Chr2g0320561 30.323 155 91 5 15 165 6 147 3.96e-14 69.7
Msa0575140 MtrunA17_Chr4g0020811 30.323 155 90 6 15 164 8 149 4.70e-13 65.1
Msa0575140 MtrunA17_Chr2g0308401 30.323 155 90 6 15 164 6 147 4.80e-13 65.1
Msa0575140 MtrunA17_Chr8g0386511 31.250 112 54 3 50 158 4 95 1.85e-11 60.5
Msa0575140 MtrunA17_Chr2g0320521 34.737 95 58 3 15 105 8 102 2.29e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575140 AT2G01818.1 43.421 152 66 4 15 161 13 149 7.00e-36 127
Msa0575140 AT3G60670.1 33.333 147 76 3 15 160 9 134 3.41e-22 92.0
Msa0575140 AT2G12646.1 33.793 145 74 3 15 158 8 131 1.08e-20 88.2
Msa0575140 AT2G12646.2 33.793 145 74 3 15 158 8 131 1.08e-20 88.2
Msa0575140 AT2G12646.3 33.793 145 74 3 15 158 5 128 1.13e-20 88.2
Msa0575140 AT1G21000.1 36.735 147 70 5 15 159 18 143 1.38e-18 82.4
Msa0575140 AT1G21000.2 36.735 147 70 5 15 159 15 140 1.40e-18 82.0
Msa0575140 AT1G43000.2 30.769 234 123 11 15 237 18 223 5.82e-18 80.1
Msa0575140 AT1G43000.1 30.769 234 123 11 15 237 11 216 7.58e-18 79.7
Msa0575140 AT4G17900.1 32.515 163 86 5 1 159 20 162 9.72e-18 79.7
Msa0575140 AT1G76590.1 38.060 134 61 4 27 159 33 145 2.07e-17 79.0
Msa0575140 AT4G17900.2 33.333 147 76 4 15 159 22 148 6.79e-17 77.0
Msa0575140 AT1G31040.1 31.579 152 81 3 15 165 21 150 8.95e-17 77.4
Msa0575140 AT2G27930.3 33.333 150 85 5 15 161 6 143 6.47e-16 73.6
Msa0575140 AT2G27930.2 33.333 150 85 5 15 161 6 143 6.47e-16 73.6
Msa0575140 AT2G27930.1 33.333 147 83 5 15 158 6 140 1.78e-15 72.8
Msa0575140 AT2G27930.4 33.333 147 83 5 15 158 6 140 1.78e-15 72.8
Msa0575140 AT5G46710.2 31.902 163 84 5 1 161 21 158 2.32e-15 72.0
Msa0575140 AT2G01818.2 39.175 97 44 2 65 161 4 85 3.17e-15 71.2
Msa0575140 AT5G46710.1 31.677 161 83 5 1 159 21 156 7.27e-15 71.6
Msa0575140 AT1G32700.1 31.447 159 86 5 2 158 1 138 8.43e-15 71.2
Msa0575140 AT5G46710.3 31.447 159 82 5 3 159 7 140 2.19e-14 70.1
Msa0575140 AT1G32700.2 35.294 119 55 4 41 158 2 99 6.53e-12 62.8

Find 69 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTGATAAGTTGCTTTCTT+TGG 0.204312 4_1:-78925693 None:intergenic
CAGGGTATCTTGAACAATTA+AGG 0.231703 4_1:+78926358 Msa0575140:three_prime_UTR
TTTATACTTGAAACCCAATA+TGG 0.234084 4_1:-78926402 None:intergenic
ATGATTCCGGTTCCGAATTA+AGG 0.235495 4_1:-78926015 None:intergenic
CTTTGACTGCTCAAATATTC+AGG 0.261064 4_1:+78925186 Msa0575140:CDS
ACTATTCGTATCAAAGTCTT+AGG 0.272266 4_1:-78926481 None:intergenic
CAATCTCTATTCCTTTCTTT+TGG 0.279770 4_1:-78924962 None:intergenic
AACTCCATTATACCCCATAT+TGG 0.300205 4_1:+78926388 Msa0575140:three_prime_UTR
AAGCTCATTCTATTCATAGA+AGG 0.351713 4_1:+78925104 Msa0575140:CDS
ACTCCATTATACCCCATATT+GGG 0.359414 4_1:+78926389 Msa0575140:three_prime_UTR
GATTTACAGATATTCCTATC+AGG 0.363443 4_1:+78925132 Msa0575140:CDS
TTCGTTTCTTAGCATATGTT+TGG 0.366680 4_1:+78926130 Msa0575140:exon
TGATTTGGACCTTGGCTTTG+AGG 0.368181 4_1:-78925896 None:intergenic
AATCAAAGGAAAAGAAATAA+AGG 0.369890 4_1:-78926299 None:intergenic
TTAATTTCATCACCATCGTT+TGG 0.373365 4_1:+78926537 Msa0575140:three_prime_UTR
AAACCCAATATGGGGTATAA+TGG 0.373437 4_1:-78926392 None:intergenic
ATAACTTCTAATAGGATGTT+TGG 0.374838 4_1:-78926254 None:intergenic
TTGAGTGATGATTTGGACCT+TGG 0.380468 4_1:-78925904 None:intergenic
TATGACTGATAACCTTAATT+CGG 0.380930 4_1:+78926003 Msa0575140:CDS
TATTTGTGATAACTTCTAAT+AGG 0.390180 4_1:-78926262 None:intergenic
GCTTCAGAAATAGATGATTC+CGG 0.410307 4_1:-78926028 None:intergenic
TTATACTTGAAACCCAATAT+GGG 0.425759 4_1:-78926401 None:intergenic
CAAAAGAAAGGAATAGAGAT+TGG 0.426289 4_1:+78924963 Msa0575140:CDS
GTTAGAATTCTCATGGTCAA+TGG 0.432521 4_1:+78926631 Msa0575140:intron
CTTACATATCGAATAAAGAT+AGG 0.448689 4_1:+78925600 Msa0575140:CDS
TGATAACCTTAATTCGGAAC+CGG 0.455008 4_1:+78926009 Msa0575140:CDS
AGTATAAACCGAGGAGTTTG+AGG 0.476733 4_1:+78926417 Msa0575140:three_prime_UTR
CATAGTAAAGTCAATAGCTT+CGG 0.480348 4_1:-78925984 None:intergenic
GACTTATAGATCGGAGGCCG+AGG 0.481284 4_1:-78925638 None:intergenic
AGGGTATCTTGAACAATTAA+GGG 0.484551 4_1:+78926359 Msa0575140:three_prime_UTR
ATGGCGGAAAACATCCTGAT+AGG 0.486299 4_1:-78925146 None:intergenic
GCTCTTATGAATAGTAGCTC+TGG 0.499499 4_1:+78924992 Msa0575140:CDS
CCGTGCGGCAATTTGAAGCT+AGG 0.504383 4_1:+78926181 Msa0575140:three_prime_UTR
ATTATCCTAAAATGATAACT+TGG 0.504942 4_1:-78926331 None:intergenic
TTATCAGCTAGATTTAAGTC+AGG 0.519647 4_1:+78925707 Msa0575140:CDS
AGGATCAATCACCAAAAGAA+AGG 0.527569 4_1:+78924951 Msa0575140:CDS
CCGATCTATAAGTCTAAAGT+TGG 0.532512 4_1:+78925647 Msa0575140:CDS
TGAAGCTGAGCCATGTGGGC+GGG 0.537407 4_1:+78926045 Msa0575140:CDS
CGTTGTGAGTAGGAAAATCA+TGG 0.557197 4_1:-78926226 None:intergenic
AGTGCTTCTGCAGCTCAGAA+TGG 0.561001 4_1:-78925165 None:intergenic
CCAACTTTAGACTTATAGAT+CGG 0.562220 4_1:-78925647 None:intergenic
ATTAAGAAATCAAGATGGGA+TGG 0.562575 4_1:-78926608 None:intergenic
GTTGAATCTTTCAACCAAAG+TGG 0.563737 4_1:+78925938 Msa0575140:CDS
TTTGGTAGCTCGTTGTGAGT+AGG 0.568439 4_1:-78926236 None:intergenic
GGCAGTACATGTGAAGAATG+TGG 0.570625 4_1:+78925731 Msa0575140:CDS
TAAGAAACGAAAACAAAGAC+AGG 0.585409 4_1:-78926118 None:intergenic
GCAGTACATGTGAAGAATGT+GGG 0.588854 4_1:+78925732 Msa0575140:CDS
CTGAAGCTGAGCCATGTGGG+CGG 0.592373 4_1:+78926044 Msa0575140:CDS
TCAGCTAGATTTAAGTCAGG+AGG 0.597898 4_1:+78925710 Msa0575140:CDS
CTGGTGCTCAATTATATGCA+AGG 0.606595 4_1:+78925781 Msa0575140:CDS
GGATTGTGCATCTCAAACCT+CGG 0.608993 4_1:+78925621 Msa0575140:CDS
TACAAGATGAGCATAGTCGC+TGG 0.613510 4_1:+78925762 Msa0575140:CDS
CAACTGTACCTCAAACTCCT+CGG 0.623775 4_1:-78926425 None:intergenic
ATAGGGGAGCCTCAAAGCCA+AGG 0.624380 4_1:+78925887 Msa0575140:CDS
TTTCTGAAGCTGAGCCATGT+GGG 0.625236 4_1:+78926041 Msa0575140:CDS
TATACTTGAAACCCAATATG+GGG 0.636266 4_1:-78926400 None:intergenic
ACAACTTCAACCCGCCCACA+TGG 0.636500 4_1:-78926055 None:intergenic
AAAGACAGGCCTCTGAGGAG+TGG 0.638363 4_1:-78926104 None:intergenic
GTGAGATGAATTCCAAACGA+TGG 0.640882 4_1:-78926549 None:intergenic
GCTTCTGCAGCTCAGAATGG+CGG 0.645960 4_1:-78925162 None:intergenic
TGATGATGCATCTACCACTT+TGG 0.646189 4_1:-78925952 None:intergenic
ATTGTGCTGTTAGATTCTGT+AGG 0.654870 4_1:+78925071 Msa0575140:CDS
ATTTCTGAAGCTGAGCCATG+TGG 0.657682 4_1:+78926040 Msa0575140:CDS
ACTTTAGACTTATAGATCGG+AGG 0.659735 4_1:-78925644 None:intergenic
AAAACAAAGACAGGCCTCTG+AGG 0.661748 4_1:-78926109 None:intergenic
CCTAGCTTCAAATTGCCGCA+CGG 0.665330 4_1:-78926181 None:intergenic
ACACAAGCAAGTAGAAAATG+CGG 0.685653 4_1:-78924423 None:intergenic
TGGGTTTCAAGTATAAACCG+AGG 0.708166 4_1:+78926408 Msa0575140:three_prime_UTR
TGTGAGTAGGAAAATCATGG+TGG 0.769697 4_1:-78926223 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAATATGAATCAAA+TGG - chr4_1:78924658-78924677 None:intergenic 10.0%
!!! TTTATTTTTGTTTTAAATGT+TGG + chr4_1:78924670-78924689 Msa0575140:intron 10.0%
!! AAAATAAAATATGAATCAAA+TGG - chr4_1:78924658-78924677 None:intergenic 10.0%
!!! TTTATTTTTGTTTTAAATGT+TGG + chr4_1:78924670-78924689 Msa0575140:intron 10.0%
!! TTATTCAATGTAATTTCAAT+AGG - chr4_1:78925387-78925406 None:intergenic 15.0%
!!! ATTGAATAAATTTTCCATAT+GGG + chr4_1:78925398-78925417 Msa0575140:intron 15.0%
!! AATAAACACAATTTATTTCA+TGG - chr4_1:78925453-78925472 None:intergenic 15.0%
!! TTATTCAATGTAATTTCAAT+AGG - chr4_1:78925387-78925406 None:intergenic 15.0%
!!! ATTGAATAAATTTTCCATAT+GGG + chr4_1:78925398-78925417 Msa0575140:intron 15.0%
!! AATAAACACAATTTATTTCA+TGG - chr4_1:78925453-78925472 None:intergenic 15.0%
!!! TATTTTGAATATCTGAGATT+AGG + chr4_1:78924772-78924791 Msa0575140:intron 20.0%
!!! TGTTTTGATTTGTTTTAATC+TGG + chr4_1:78925312-78925331 Msa0575140:intron 20.0%
!!! CATTGAATAAATTTTCCATA+TGG + chr4_1:78925397-78925416 Msa0575140:intron 20.0%
!! AATTTATAATTCAACCCATA+TGG - chr4_1:78925415-78925434 None:intergenic 20.0%
!! AGTTTATGATCAATAACAAT+TGG - chr4_1:78925525-78925544 None:intergenic 20.0%
!! TATTTGTGATAACTTCTAAT+AGG - chr4_1:78926265-78926284 None:intergenic 20.0%
!! AATCAAAGGAAAAGAAATAA+AGG - chr4_1:78926302-78926321 None:intergenic 20.0%
!! ATTATCCTAAAATGATAACT+TGG - chr4_1:78926334-78926353 None:intergenic 20.0%
!! TAACATTAAGAAATCAAGAT+GGG - chr4_1:78926615-78926634 None:intergenic 20.0%
!!! TATTTTGAATATCTGAGATT+AGG + chr4_1:78924772-78924791 Msa0575140:intron 20.0%
!!! TGTTTTGATTTGTTTTAATC+TGG + chr4_1:78925312-78925331 Msa0575140:intron 20.0%
!!! CATTGAATAAATTTTCCATA+TGG + chr4_1:78925397-78925416 Msa0575140:intron 20.0%
!! AATTTATAATTCAACCCATA+TGG - chr4_1:78925415-78925434 None:intergenic 20.0%
!! AGTTTATGATCAATAACAAT+TGG - chr4_1:78925525-78925544 None:intergenic 20.0%
!! TATTTGTGATAACTTCTAAT+AGG - chr4_1:78926265-78926284 None:intergenic 20.0%
!! AATCAAAGGAAAAGAAATAA+AGG - chr4_1:78926302-78926321 None:intergenic 20.0%
!! ATTATCCTAAAATGATAACT+TGG - chr4_1:78926334-78926353 None:intergenic 20.0%
!! TAACATTAAGAAATCAAGAT+GGG - chr4_1:78926615-78926634 None:intergenic 20.0%
! AAAAAGAGAGAATGTGAAAA+GGG - chr4_1:78924372-78924391 None:intergenic 25.0%
!! AATTGTTCTTACCATGTTTT+TGG - chr4_1:78924462-78924481 None:intergenic 25.0%
! CAAAACTTTATAAGAATCCA+TGG - chr4_1:78924504-78924523 None:intergenic 25.0%
!! CTGATTCTGAATTAAGTTTT+TGG + chr4_1:78924870-78924889 Msa0575140:intron 25.0%
!!! TTCTGAATTAAGTTTTTGGT+TGG + chr4_1:78924874-78924893 Msa0575140:intron 25.0%
!!! TCTGAATTAAGTTTTTGGTT+GGG + chr4_1:78924875-78924894 Msa0575140:intron 25.0%
!!! TTGATTTGTTTTAATCTGGT+TGG + chr4_1:78925316-78925335 Msa0575140:intron 25.0%
! CTTACATATCGAATAAAGAT+AGG + chr4_1:78925600-78925619 Msa0575140:CDS 25.0%
!!! TTTCTTTGGATTTTGATTCA+GGG - chr4_1:78925682-78925701 None:intergenic 25.0%
!! ACAAACATGCTCTTATTTAA+TGG + chr4_1:78925806-78925825 Msa0575140:intron 25.0%
! TATGACTGATAACCTTAATT+CGG + chr4_1:78926003-78926022 Msa0575140:CDS 25.0%
! ATAACTTCTAATAGGATGTT+TGG - chr4_1:78926257-78926276 None:intergenic 25.0%
! TTATACTTGAAACCCAATAT+GGG - chr4_1:78926404-78926423 None:intergenic 25.0%
! TTTATACTTGAAACCCAATA+TGG - chr4_1:78926405-78926424 None:intergenic 25.0%
! CTAACATTAAGAAATCAAGA+TGG - chr4_1:78926616-78926635 None:intergenic 25.0%
! TCTTAATGTTAGAATTCTCA+TGG + chr4_1:78926624-78926643 Msa0575140:intron 25.0%
! AAAAAGAGAGAATGTGAAAA+GGG - chr4_1:78924372-78924391 None:intergenic 25.0%
!! AATTGTTCTTACCATGTTTT+TGG - chr4_1:78924462-78924481 None:intergenic 25.0%
! CAAAACTTTATAAGAATCCA+TGG - chr4_1:78924504-78924523 None:intergenic 25.0%
!! CTGATTCTGAATTAAGTTTT+TGG + chr4_1:78924870-78924889 Msa0575140:intron 25.0%
!!! TTCTGAATTAAGTTTTTGGT+TGG + chr4_1:78924874-78924893 Msa0575140:intron 25.0%
!!! TCTGAATTAAGTTTTTGGTT+GGG + chr4_1:78924875-78924894 Msa0575140:intron 25.0%
!!! TTGATTTGTTTTAATCTGGT+TGG + chr4_1:78925316-78925335 Msa0575140:intron 25.0%
! CTTACATATCGAATAAAGAT+AGG + chr4_1:78925600-78925619 Msa0575140:CDS 25.0%
!!! TTTCTTTGGATTTTGATTCA+GGG - chr4_1:78925682-78925701 None:intergenic 25.0%
!! ACAAACATGCTCTTATTTAA+TGG + chr4_1:78925806-78925825 Msa0575140:intron 25.0%
! TATGACTGATAACCTTAATT+CGG + chr4_1:78926003-78926022 Msa0575140:CDS 25.0%
! ATAACTTCTAATAGGATGTT+TGG - chr4_1:78926257-78926276 None:intergenic 25.0%
! TTATACTTGAAACCCAATAT+GGG - chr4_1:78926404-78926423 None:intergenic 25.0%
! TTTATACTTGAAACCCAATA+TGG - chr4_1:78926405-78926424 None:intergenic 25.0%
! CTAACATTAAGAAATCAAGA+TGG - chr4_1:78926616-78926635 None:intergenic 25.0%
! TCTTAATGTTAGAATTCTCA+TGG + chr4_1:78926624-78926643 Msa0575140:intron 25.0%
GAAAAAGAGAGAATGTGAAA+AGG - chr4_1:78924373-78924392 None:intergenic 30.0%
!!! CATGTTTTTGGTTTGAGATT+GGG - chr4_1:78924450-78924469 None:intergenic 30.0%
!!! TCAATTTCCTGTTTTTTCCA+TGG + chr4_1:78924484-78924503 Msa0575140:intron 30.0%
TAAGAATCCATGGAAAAAAC+AGG - chr4_1:78924494-78924513 None:intergenic 30.0%
AACGAAATCAGAATCAGAAT+AGG - chr4_1:78924535-78924554 None:intergenic 30.0%
GAAATTTGCATGTGAAGATT+TGG + chr4_1:78924734-78924753 Msa0575140:intron 30.0%
! GAATATCTGAGATTAGGTTT+AGG + chr4_1:78924778-78924797 Msa0575140:intron 30.0%
!! GGTTCTTGAATTGATTTTGT+GGG + chr4_1:78924896-78924915 Msa0575140:intron 30.0%
!!! TGAATAATGATGGATTTTGC+AGG + chr4_1:78924922-78924941 Msa0575140:intron 30.0%
! CAATCTCTATTCCTTTCTTT+TGG - chr4_1:78924965-78924984 None:intergenic 30.0%
CAAAAGAAAGGAATAGAGAT+TGG + chr4_1:78924963-78924982 Msa0575140:CDS 30.0%
AAGCTCATTCTATTCATAGA+AGG + chr4_1:78925104-78925123 Msa0575140:CDS 30.0%
GATTTACAGATATTCCTATC+AGG + chr4_1:78925132-78925151 Msa0575140:CDS 30.0%
GATAGACTTTAATGATCTGA+TGG + chr4_1:78925340-78925359 Msa0575140:intron 30.0%
TCAATAGGTTCAACCATTAA+TGG - chr4_1:78925372-78925391 None:intergenic 30.0%
AACAAACAATGACAGTACAA+AGG - chr4_1:78925479-78925498 None:intergenic 30.0%
CCAACTTTAGACTTATAGAT+CGG - chr4_1:78925650-78925669 None:intergenic 30.0%
!!! CTTTCTTTGGATTTTGATTC+AGG - chr4_1:78925683-78925702 None:intergenic 30.0%
TTATCAGCTAGATTTAAGTC+AGG + chr4_1:78925707-78925726 Msa0575140:CDS 30.0%
!!! CTTCTTTTTGAGTGATGATT+TGG - chr4_1:78925914-78925933 None:intergenic 30.0%
CATAGTAAAGTCAATAGCTT+CGG - chr4_1:78925987-78926006 None:intergenic 30.0%
TAAGAAACGAAAACAAAGAC+AGG - chr4_1:78926121-78926140 None:intergenic 30.0%
TTCGTTTCTTAGCATATGTT+TGG + chr4_1:78926130-78926149 Msa0575140:exon 30.0%
CTTGGAAGCAAAAAAATCAA+AGG - chr4_1:78926316-78926335 None:intergenic 30.0%
! TGCTTCCAAGTTATCATTTT+AGG + chr4_1:78926326-78926345 Msa0575140:three_prime_UTR 30.0%
!!! TCATTTTAGGATAATGAGAC+AGG + chr4_1:78926339-78926358 Msa0575140:three_prime_UTR 30.0%
!!! CATTTTAGGATAATGAGACA+GGG + chr4_1:78926340-78926359 Msa0575140:three_prime_UTR 30.0%
! AGGGTATCTTGAACAATTAA+GGG + chr4_1:78926359-78926378 Msa0575140:three_prime_UTR 30.0%
TATACTTGAAACCCAATATG+GGG - chr4_1:78926403-78926422 None:intergenic 30.0%
ACTATTCGTATCAAAGTCTT+AGG - chr4_1:78926484-78926503 None:intergenic 30.0%
TTAATTTCATCACCATCGTT+TGG + chr4_1:78926537-78926556 Msa0575140:three_prime_UTR 30.0%
ATTAAGAAATCAAGATGGGA+TGG - chr4_1:78926611-78926630 None:intergenic 30.0%
GAAAAAGAGAGAATGTGAAA+AGG - chr4_1:78924373-78924392 None:intergenic 30.0%
!!! CATGTTTTTGGTTTGAGATT+GGG - chr4_1:78924450-78924469 None:intergenic 30.0%
!!! TCAATTTCCTGTTTTTTCCA+TGG + chr4_1:78924484-78924503 Msa0575140:intron 30.0%
TAAGAATCCATGGAAAAAAC+AGG - chr4_1:78924494-78924513 None:intergenic 30.0%
AACGAAATCAGAATCAGAAT+AGG - chr4_1:78924535-78924554 None:intergenic 30.0%
GAAATTTGCATGTGAAGATT+TGG + chr4_1:78924734-78924753 Msa0575140:intron 30.0%
! GAATATCTGAGATTAGGTTT+AGG + chr4_1:78924778-78924797 Msa0575140:intron 30.0%
!! GGTTCTTGAATTGATTTTGT+GGG + chr4_1:78924896-78924915 Msa0575140:intron 30.0%
!!! TGAATAATGATGGATTTTGC+AGG + chr4_1:78924922-78924941 Msa0575140:intron 30.0%
! CAATCTCTATTCCTTTCTTT+TGG - chr4_1:78924965-78924984 None:intergenic 30.0%
CAAAAGAAAGGAATAGAGAT+TGG + chr4_1:78924963-78924982 Msa0575140:CDS 30.0%
AAGCTCATTCTATTCATAGA+AGG + chr4_1:78925104-78925123 Msa0575140:CDS 30.0%
GATTTACAGATATTCCTATC+AGG + chr4_1:78925132-78925151 Msa0575140:CDS 30.0%
GATAGACTTTAATGATCTGA+TGG + chr4_1:78925340-78925359 Msa0575140:intron 30.0%
TCAATAGGTTCAACCATTAA+TGG - chr4_1:78925372-78925391 None:intergenic 30.0%
AACAAACAATGACAGTACAA+AGG - chr4_1:78925479-78925498 None:intergenic 30.0%
CCAACTTTAGACTTATAGAT+CGG - chr4_1:78925650-78925669 None:intergenic 30.0%
!!! CTTTCTTTGGATTTTGATTC+AGG - chr4_1:78925683-78925702 None:intergenic 30.0%
TTATCAGCTAGATTTAAGTC+AGG + chr4_1:78925707-78925726 Msa0575140:CDS 30.0%
!!! CTTCTTTTTGAGTGATGATT+TGG - chr4_1:78925914-78925933 None:intergenic 30.0%
CATAGTAAAGTCAATAGCTT+CGG - chr4_1:78925987-78926006 None:intergenic 30.0%
TAAGAAACGAAAACAAAGAC+AGG - chr4_1:78926121-78926140 None:intergenic 30.0%
TTCGTTTCTTAGCATATGTT+TGG + chr4_1:78926130-78926149 Msa0575140:exon 30.0%
CTTGGAAGCAAAAAAATCAA+AGG - chr4_1:78926316-78926335 None:intergenic 30.0%
! TGCTTCCAAGTTATCATTTT+AGG + chr4_1:78926326-78926345 Msa0575140:three_prime_UTR 30.0%
!!! TCATTTTAGGATAATGAGAC+AGG + chr4_1:78926339-78926358 Msa0575140:three_prime_UTR 30.0%
!!! CATTTTAGGATAATGAGACA+GGG + chr4_1:78926340-78926359 Msa0575140:three_prime_UTR 30.0%
! AGGGTATCTTGAACAATTAA+GGG + chr4_1:78926359-78926378 Msa0575140:three_prime_UTR 30.0%
TATACTTGAAACCCAATATG+GGG - chr4_1:78926403-78926422 None:intergenic 30.0%
ACTATTCGTATCAAAGTCTT+AGG - chr4_1:78926484-78926503 None:intergenic 30.0%
TTAATTTCATCACCATCGTT+TGG + chr4_1:78926537-78926556 Msa0575140:three_prime_UTR 30.0%
ATTAAGAAATCAAGATGGGA+TGG - chr4_1:78926611-78926630 None:intergenic 30.0%
ACACAAGCAAGTAGAAAATG+CGG - chr4_1:78924426-78924445 None:intergenic 35.0%
!!! CCATGTTTTTGGTTTGAGAT+TGG - chr4_1:78924451-78924470 None:intergenic 35.0%
CCAATCTCAAACCAAAAACA+TGG + chr4_1:78924448-78924467 Msa0575140:exon 35.0%
!! GGGTTCTTGAATTGATTTTG+TGG + chr4_1:78924895-78924914 Msa0575140:intron 35.0%
!! TTGTGGGTACTGAATAATGA+TGG + chr4_1:78924912-78924931 Msa0575140:intron 35.0%
!!! ATAATGATGGATTTTGCAGG+TGG + chr4_1:78924925-78924944 Msa0575140:intron 35.0%
!!! TAATGATGGATTTTGCAGGT+GGG + chr4_1:78924926-78924945 Msa0575140:intron 35.0%
AGGATCAATCACCAAAAGAA+AGG + chr4_1:78924951-78924970 Msa0575140:CDS 35.0%
! ATTGTGCTGTTAGATTCTGT+AGG + chr4_1:78925071-78925090 Msa0575140:CDS 35.0%
CTTTGACTGCTCAAATATTC+AGG + chr4_1:78925186-78925205 Msa0575140:CDS 35.0%
! CTGATGGACATTACCATTAA+TGG + chr4_1:78925356-78925375 Msa0575140:intron 35.0%
CGTACAATTGTTCCATGATT+TGG + chr4_1:78925573-78925592 Msa0575140:intron 35.0%
TATGTAAGTCTGCCAAATCA+TGG - chr4_1:78925588-78925607 None:intergenic 35.0%
ACTTTAGACTTATAGATCGG+AGG - chr4_1:78925647-78925666 None:intergenic 35.0%
! CCGATCTATAAGTCTAAAGT+TGG + chr4_1:78925647-78925666 Msa0575140:CDS 35.0%
! AGCTGATAAGTTGCTTTCTT+TGG - chr4_1:78925696-78925715 None:intergenic 35.0%
! ATCATGTGCTGTTGTTAGAT+AGG + chr4_1:78925869-78925888 Msa0575140:intron 35.0%
! TCATGTGCTGTTGTTAGATA+GGG + chr4_1:78925870-78925889 Msa0575140:intron 35.0%
GTTGAATCTTTCAACCAAAG+TGG + chr4_1:78925938-78925957 Msa0575140:CDS 35.0%
! AAGTCAATAGCTTCGGTTTT+CGG - chr4_1:78925980-78925999 None:intergenic 35.0%
TGATAACCTTAATTCGGAAC+CGG + chr4_1:78926009-78926028 Msa0575140:CDS 35.0%
GCTTCAGAAATAGATGATTC+CGG - chr4_1:78926031-78926050 None:intergenic 35.0%
! CAGGGTATCTTGAACAATTA+AGG + chr4_1:78926358-78926377 Msa0575140:three_prime_UTR 35.0%
AACTCCATTATACCCCATAT+TGG + chr4_1:78926388-78926407 Msa0575140:three_prime_UTR 35.0%
ACTCCATTATACCCCATATT+GGG + chr4_1:78926389-78926408 Msa0575140:three_prime_UTR 35.0%
AAACCCAATATGGGGTATAA+TGG - chr4_1:78926395-78926414 None:intergenic 35.0%
GTTAGAATTCTCATGGTCAA+TGG + chr4_1:78926631-78926650 Msa0575140:intron 35.0%
ACACAAGCAAGTAGAAAATG+CGG - chr4_1:78924426-78924445 None:intergenic 35.0%
!!! CCATGTTTTTGGTTTGAGAT+TGG - chr4_1:78924451-78924470 None:intergenic 35.0%
CCAATCTCAAACCAAAAACA+TGG + chr4_1:78924448-78924467 Msa0575140:exon 35.0%
!! GGGTTCTTGAATTGATTTTG+TGG + chr4_1:78924895-78924914 Msa0575140:intron 35.0%
!! TTGTGGGTACTGAATAATGA+TGG + chr4_1:78924912-78924931 Msa0575140:intron 35.0%
!!! ATAATGATGGATTTTGCAGG+TGG + chr4_1:78924925-78924944 Msa0575140:intron 35.0%
!!! TAATGATGGATTTTGCAGGT+GGG + chr4_1:78924926-78924945 Msa0575140:intron 35.0%
AGGATCAATCACCAAAAGAA+AGG + chr4_1:78924951-78924970 Msa0575140:CDS 35.0%
! ATTGTGCTGTTAGATTCTGT+AGG + chr4_1:78925071-78925090 Msa0575140:CDS 35.0%
CTTTGACTGCTCAAATATTC+AGG + chr4_1:78925186-78925205 Msa0575140:CDS 35.0%
! CTGATGGACATTACCATTAA+TGG + chr4_1:78925356-78925375 Msa0575140:intron 35.0%
CGTACAATTGTTCCATGATT+TGG + chr4_1:78925573-78925592 Msa0575140:intron 35.0%
TATGTAAGTCTGCCAAATCA+TGG - chr4_1:78925588-78925607 None:intergenic 35.0%
ACTTTAGACTTATAGATCGG+AGG - chr4_1:78925647-78925666 None:intergenic 35.0%
! CCGATCTATAAGTCTAAAGT+TGG + chr4_1:78925647-78925666 Msa0575140:CDS 35.0%
! AGCTGATAAGTTGCTTTCTT+TGG - chr4_1:78925696-78925715 None:intergenic 35.0%
! ATCATGTGCTGTTGTTAGAT+AGG + chr4_1:78925869-78925888 Msa0575140:intron 35.0%
! TCATGTGCTGTTGTTAGATA+GGG + chr4_1:78925870-78925889 Msa0575140:intron 35.0%
GTTGAATCTTTCAACCAAAG+TGG + chr4_1:78925938-78925957 Msa0575140:CDS 35.0%
! AAGTCAATAGCTTCGGTTTT+CGG - chr4_1:78925980-78925999 None:intergenic 35.0%
TGATAACCTTAATTCGGAAC+CGG + chr4_1:78926009-78926028 Msa0575140:CDS 35.0%
GCTTCAGAAATAGATGATTC+CGG - chr4_1:78926031-78926050 None:intergenic 35.0%
! CAGGGTATCTTGAACAATTA+AGG + chr4_1:78926358-78926377 Msa0575140:three_prime_UTR 35.0%
AACTCCATTATACCCCATAT+TGG + chr4_1:78926388-78926407 Msa0575140:three_prime_UTR 35.0%
ACTCCATTATACCCCATATT+GGG + chr4_1:78926389-78926408 Msa0575140:three_prime_UTR 35.0%
AAACCCAATATGGGGTATAA+TGG - chr4_1:78926395-78926414 None:intergenic 35.0%
GTTAGAATTCTCATGGTCAA+TGG + chr4_1:78926631-78926650 Msa0575140:intron 35.0%
!!! AATGATGGATTTTGCAGGTG+GGG + chr4_1:78924927-78924946 Msa0575140:intron 40.0%
GCTCTTATGAATAGTAGCTC+TGG + chr4_1:78924992-78925011 Msa0575140:CDS 40.0%
TCAGCTAGATTTAAGTCAGG+AGG + chr4_1:78925710-78925729 Msa0575140:CDS 40.0%
GCAGTACATGTGAAGAATGT+GGG + chr4_1:78925732-78925751 Msa0575140:CDS 40.0%
CTGGTGCTCAATTATATGCA+AGG + chr4_1:78925781-78925800 Msa0575140:CDS 40.0%
! CATGTGCTGTTGTTAGATAG+GGG + chr4_1:78925871-78925890 Msa0575140:intron 40.0%
! TTGAGTGATGATTTGGACCT+TGG - chr4_1:78925907-78925926 None:intergenic 40.0%
! TGATGATGCATCTACCACTT+TGG - chr4_1:78925955-78925974 None:intergenic 40.0%
ATGATTCCGGTTCCGAATTA+AGG - chr4_1:78926018-78926037 None:intergenic 40.0%
TGTGAGTAGGAAAATCATGG+TGG - chr4_1:78926226-78926245 None:intergenic 40.0%
CGTTGTGAGTAGGAAAATCA+TGG - chr4_1:78926229-78926248 None:intergenic 40.0%
! TGGGTTTCAAGTATAAACCG+AGG + chr4_1:78926408-78926427 Msa0575140:three_prime_UTR 40.0%
AGTATAAACCGAGGAGTTTG+AGG + chr4_1:78926417-78926436 Msa0575140:three_prime_UTR 40.0%
GTGAGATGAATTCCAAACGA+TGG - chr4_1:78926552-78926571 None:intergenic 40.0%
!!! AATGATGGATTTTGCAGGTG+GGG + chr4_1:78924927-78924946 Msa0575140:intron 40.0%
GCTCTTATGAATAGTAGCTC+TGG + chr4_1:78924992-78925011 Msa0575140:CDS 40.0%
TCAGCTAGATTTAAGTCAGG+AGG + chr4_1:78925710-78925729 Msa0575140:CDS 40.0%
GCAGTACATGTGAAGAATGT+GGG + chr4_1:78925732-78925751 Msa0575140:CDS 40.0%
CTGGTGCTCAATTATATGCA+AGG + chr4_1:78925781-78925800 Msa0575140:CDS 40.0%
! CATGTGCTGTTGTTAGATAG+GGG + chr4_1:78925871-78925890 Msa0575140:intron 40.0%
! TTGAGTGATGATTTGGACCT+TGG - chr4_1:78925907-78925926 None:intergenic 40.0%
! TGATGATGCATCTACCACTT+TGG - chr4_1:78925955-78925974 None:intergenic 40.0%
ATGATTCCGGTTCCGAATTA+AGG - chr4_1:78926018-78926037 None:intergenic 40.0%
TGTGAGTAGGAAAATCATGG+TGG - chr4_1:78926226-78926245 None:intergenic 40.0%
CGTTGTGAGTAGGAAAATCA+TGG - chr4_1:78926229-78926248 None:intergenic 40.0%
! TGGGTTTCAAGTATAAACCG+AGG + chr4_1:78926408-78926427 Msa0575140:three_prime_UTR 40.0%
AGTATAAACCGAGGAGTTTG+AGG + chr4_1:78926417-78926436 Msa0575140:three_prime_UTR 40.0%
GTGAGATGAATTCCAAACGA+TGG - chr4_1:78926552-78926571 None:intergenic 40.0%
! ATGGCGGAAAACATCCTGAT+AGG - chr4_1:78925149-78925168 None:intergenic 45.0%
GGATTGTGCATCTCAAACCT+CGG + chr4_1:78925621-78925640 Msa0575140:CDS 45.0%
GGCAGTACATGTGAAGAATG+TGG + chr4_1:78925731-78925750 Msa0575140:CDS 45.0%
TACAAGATGAGCATAGTCGC+TGG + chr4_1:78925762-78925781 Msa0575140:CDS 45.0%
!! TGATTTGGACCTTGGCTTTG+AGG - chr4_1:78925899-78925918 None:intergenic 45.0%
!! ATTTCTGAAGCTGAGCCATG+TGG + chr4_1:78926040-78926059 Msa0575140:CDS 45.0%
!! TTTCTGAAGCTGAGCCATGT+GGG + chr4_1:78926041-78926060 Msa0575140:CDS 45.0%
! TCTGAGGAGTGGTTTTTCTC+GGG - chr4_1:78926096-78926115 None:intergenic 45.0%
!! CTCTGAGGAGTGGTTTTTCT+CGG - chr4_1:78926097-78926116 None:intergenic 45.0%
AAAACAAAGACAGGCCTCTG+AGG - chr4_1:78926112-78926131 None:intergenic 45.0%
! ATTTTCACAGCATCACCGTG+CGG + chr4_1:78926166-78926185 Msa0575140:three_prime_UTR 45.0%
! TTTGGTAGCTCGTTGTGAGT+AGG - chr4_1:78926239-78926258 None:intergenic 45.0%
CAACTGTACCTCAAACTCCT+CGG - chr4_1:78926428-78926447 None:intergenic 45.0%
! ATGGCGGAAAACATCCTGAT+AGG - chr4_1:78925149-78925168 None:intergenic 45.0%
GGATTGTGCATCTCAAACCT+CGG + chr4_1:78925621-78925640 Msa0575140:CDS 45.0%
GGCAGTACATGTGAAGAATG+TGG + chr4_1:78925731-78925750 Msa0575140:CDS 45.0%
TACAAGATGAGCATAGTCGC+TGG + chr4_1:78925762-78925781 Msa0575140:CDS 45.0%
!! TGATTTGGACCTTGGCTTTG+AGG - chr4_1:78925899-78925918 None:intergenic 45.0%
!! ATTTCTGAAGCTGAGCCATG+TGG + chr4_1:78926040-78926059 Msa0575140:CDS 45.0%
!! TTTCTGAAGCTGAGCCATGT+GGG + chr4_1:78926041-78926060 Msa0575140:CDS 45.0%
! TCTGAGGAGTGGTTTTTCTC+GGG - chr4_1:78926096-78926115 None:intergenic 45.0%
!! CTCTGAGGAGTGGTTTTTCT+CGG - chr4_1:78926097-78926116 None:intergenic 45.0%
AAAACAAAGACAGGCCTCTG+AGG - chr4_1:78926112-78926131 None:intergenic 45.0%
! ATTTTCACAGCATCACCGTG+CGG + chr4_1:78926166-78926185 Msa0575140:three_prime_UTR 45.0%
! TTTGGTAGCTCGTTGTGAGT+AGG - chr4_1:78926239-78926258 None:intergenic 45.0%
CAACTGTACCTCAAACTCCT+CGG - chr4_1:78926428-78926447 None:intergenic 45.0%
! ATGGATTTTGCAGGTGGGGA+AGG + chr4_1:78924931-78924950 Msa0575140:intron 50.0%
AGTGCTTCTGCAGCTCAGAA+TGG - chr4_1:78925168-78925187 None:intergenic 50.0%
CGAGAAAAACCACTCCTCAG+AGG + chr4_1:78926095-78926114 Msa0575140:CDS 50.0%
CCTAGCTTCAAATTGCCGCA+CGG - chr4_1:78926184-78926203 None:intergenic 50.0%
! ATGGATTTTGCAGGTGGGGA+AGG + chr4_1:78924931-78924950 Msa0575140:intron 50.0%
AGTGCTTCTGCAGCTCAGAA+TGG - chr4_1:78925168-78925187 None:intergenic 50.0%
CGAGAAAAACCACTCCTCAG+AGG + chr4_1:78926095-78926114 Msa0575140:CDS 50.0%
CCTAGCTTCAAATTGCCGCA+CGG - chr4_1:78926184-78926203 None:intergenic 50.0%
GCTTCTGCAGCTCAGAATGG+CGG - chr4_1:78925165-78925184 None:intergenic 55.0%
GACTTATAGATCGGAGGCCG+AGG - chr4_1:78925641-78925660 None:intergenic 55.0%
ATAGGGGAGCCTCAAAGCCA+AGG + chr4_1:78925887-78925906 Msa0575140:CDS 55.0%
ACAACTTCAACCCGCCCACA+TGG - chr4_1:78926058-78926077 None:intergenic 55.0%
! AAAGACAGGCCTCTGAGGAG+TGG - chr4_1:78926107-78926126 None:intergenic 55.0%
! CCGTGCGGCAATTTGAAGCT+AGG + chr4_1:78926181-78926200 Msa0575140:three_prime_UTR 55.0%
GCTTCTGCAGCTCAGAATGG+CGG - chr4_1:78925165-78925184 None:intergenic 55.0%
GACTTATAGATCGGAGGCCG+AGG - chr4_1:78925641-78925660 None:intergenic 55.0%
ATAGGGGAGCCTCAAAGCCA+AGG + chr4_1:78925887-78925906 Msa0575140:CDS 55.0%
ACAACTTCAACCCGCCCACA+TGG - chr4_1:78926058-78926077 None:intergenic 55.0%
! AAAGACAGGCCTCTGAGGAG+TGG - chr4_1:78926107-78926126 None:intergenic 55.0%
! CCGTGCGGCAATTTGAAGCT+AGG + chr4_1:78926181-78926200 Msa0575140:three_prime_UTR 55.0%
CTGAAGCTGAGCCATGTGGG+CGG + chr4_1:78926044-78926063 Msa0575140:CDS 60.0%
TGAAGCTGAGCCATGTGGGC+GGG + chr4_1:78926045-78926064 Msa0575140:CDS 60.0%
CTGAAGCTGAGCCATGTGGG+CGG + chr4_1:78926044-78926063 Msa0575140:CDS 60.0%
TGAAGCTGAGCCATGTGGGC+GGG + chr4_1:78926045-78926064 Msa0575140:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 78924348 78926687 78924348 ID=Msa0575140;Name=Msa0575140
chr4_1 mRNA 78924348 78926687 78924348 ID=Msa0575140-mRNA-1;Parent=Msa0575140;Name=Msa0575140-mRNA-1;_AED=0.07;_eAED=0.07;_QI=119|1|1|1|0.75|0.6|5|518|241
chr4_1 exon 78924348 78924469 78924348 ID=Msa0575140-mRNA-1:exon:28046;Parent=Msa0575140-mRNA-1
chr4_1 exon 78924944 78925207 78924944 ID=Msa0575140-mRNA-1:exon:28047;Parent=Msa0575140-mRNA-1
chr4_1 exon 78925599 78925802 78925599 ID=Msa0575140-mRNA-1:exon:28048;Parent=Msa0575140-mRNA-1
chr4_1 exon 78925887 78926616 78925887 ID=Msa0575140-mRNA-1:exon:28049;Parent=Msa0575140-mRNA-1
chr4_1 exon 78926645 78926687 78926645 ID=Msa0575140-mRNA-1:exon:28050;Parent=Msa0575140-mRNA-1
chr4_1 five_prime_UTR 78924348 78924466 78924348 ID=Msa0575140-mRNA-1:five_prime_utr;Parent=Msa0575140-mRNA-1
chr4_1 CDS 78924467 78924469 78924467 ID=Msa0575140-mRNA-1:cds;Parent=Msa0575140-mRNA-1
chr4_1 CDS 78924944 78925207 78924944 ID=Msa0575140-mRNA-1:cds;Parent=Msa0575140-mRNA-1
chr4_1 CDS 78925599 78925802 78925599 ID=Msa0575140-mRNA-1:cds;Parent=Msa0575140-mRNA-1
chr4_1 CDS 78925887 78926141 78925887 ID=Msa0575140-mRNA-1:cds;Parent=Msa0575140-mRNA-1
chr4_1 three_prime_UTR 78926142 78926616 78926142 ID=Msa0575140-mRNA-1:three_prime_utr;Parent=Msa0575140-mRNA-1
chr4_1 three_prime_UTR 78926645 78926687 78926645 ID=Msa0575140-mRNA-1:three_prime_utr;Parent=Msa0575140-mRNA-1
Gene Sequence

>Msa0575140

ATGGTGGGGAAGGATCAATCACCAAAAGAAAGGAATAGAGATTGGATTAGAGCTCTTATGAATAGTAGCTCTGGCTATTGTGATGATCATCGCGATCTTCGATCTAATGAGAAGAATACTTTCTGTGTAGATTGTGCTGTTAGATTCTGTAGGCACTGTAAAGAAGCTCATTCTATTCATAGAAGGTTTCAGATTTACAGATATTCCTATCAGGATGTTTTCCGCCATTCTGAGCTGCAGAAGCACTTTGACTGCTCAAATATTCAGACTTACATATCGAATAAAGATAGGATTGTGCATCTCAAACCTCGGCCTCCGATCTATAAGTCTAAAGTTGGTGATCAATGCCCTGAATCAAAATCCAAAGAAAGCAACTTATCAGCTAGATTTAAGTCAGGAGGCAGTACATGTGAAGAATGTGGGAAACACTTACAAGATGAGCATAGTCGCTGGTGCTCAATTATATGCAAGATAGGGGAGCCTCAAAGCCAAGGTCCAAATCATCACTCAAAAAGAAGTTTAGTTGAATCTTTCAACCAAAGTGGTAGATGCATCATCACTCCGAAAACCGAAGCTATTGACTTTACTATGACTGATAACCTTAATTCGGAACCGGAATCATCTATTTCTGAAGCTGAGCCATGTGGGCGGGTTGAAGTTGTGAATTTCAGAAAGCGCCCGAGAAAAACCACTCCTCAGAGGCCTGTCTTTGTTTTCGTTTCTTAG
Protein sequence

>Msa0575140

MVGKDQSPKERNRDWIRALMNSSSGYCDDHRDLRSNEKNTFCVDCAVRFCRHCKEAHSIHRRFQIYRYSYQDVFRHSELQKHFDCSNIQTYISNKDRIVHLKPRPPIYKSKVGDQCPESKSKESNLSARFKSGGSTCEECGKHLQDEHSRWCSIICKIGEPQSQGPNHHSKRSLVESFNQSGRCIITPKTEAIDFTMTDNLNSEPESSISEAEPCGRVEVVNFRKRPRKTTPQRPVFVFVS*