AlfalfaGEDB | Msa0575600

M. sativa cultivar zhongmu-4 / Msa0575600

KEGG: K12606|K13754
GO: GO:0000003 GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000932 GO:0000956 GO:0001932 GO:0001934 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005102 GO:0005154 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007044 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007166 GO:0007276 GO:0007283 GO:0007346 GO:0007548 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010562 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0010648 GO:0010948 GO:0016043 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019220 GO:0019221 GO:0019222 GO:0019439 GO:0019899 GO:0019900 GO:0019904 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0030014 GO:0030015 GO:0030330 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031401 GO:0031570 GO:0031571 GO:0031581 GO:0032268 GO:0032269 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0033135 GO:0033138 GO:0033143 GO:0033144 GO:0033146 GO:0033147 GO:0033554 GO:0034097 GO:0034243 GO:0034248 GO:0034249 GO:0034329 GO:0034330 GO:0034641 GO:0034655 GO:0035556 GO:0035770 GO:0036464 GO:0042058 GO:0042221 GO:0042325 GO:0042327 GO:0042770 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045742 GO:0045786 GO:0045787 GO:0045893 GO:0045930 GO:0045935 GO:0045937 GO:0045944 GO:0046483 GO:0046700 GO:0046983 GO:0048232 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048609 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051254 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070851 GO:0070887 GO:0071156 GO:0071158 GO:0071310 GO:0071345 GO:0071704 GO:0071840 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:1901184 GO:1901186 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902680 GO:1902806 GO:1902807 GO:1903047 GO:1903506 GO:1903508 GO:1990904 GO:2000045 GO:2000112 GO:2000113 GO:2000134 GO:2000325 GO:2000327 GO:2001141
PFAM: PF04078.16

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575600	XP_003619723.2	89.783	323	33	0	1	323	1	323	0.0	598

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575600	sp\|Q6P819\|CNOT9_XENTR	56.538	260	113	0	43	302	28	287	2.93e-98	295

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575600	G7KNV7	89.783	323	33	0	1	323	1	323	0.0	598

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TFs/TRs:

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Gene ID	Type	Classification
Msa0575600	TR	Rcd1-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575600	MtrunA17_Chr8g0392781	81.006	358	33	2	1	323	1	358	0.0	573
Msa0575600	MtrunA17_Chr6g0477171	89.055	201	22	0	59	259	45	245	6.33e-129	368
Msa0575600	MtrunA17_Chr7g0224601	63.158	304	108	2	11	310	1	304	2.69e-125	361
Msa0575600	MtrunA17_Chr3g0099561	63.082	279	103	0	38	316	26	304	8.65e-119	344
Msa0575600	MtrunA17_Chr1g0177151	58.366	257	103	1	1	257	1	253	6.18e-102	300
Msa0575600	MtrunA17_Chr5g0444621	51.373	255	116	2	27	279	13	261	7.83e-77	236
Msa0575600	MtrunA17_Chr3g0083811	50.862	116	57	0	186	301	1	116	2.80e-35	124
Msa0575600	MtrunA17_Chr3g0083821	69.048	84	26	0	81	164	37	120	5.51e-32	116
Msa0575600	MtrunA17_Chr6g0477181	88.136	59	7	0	1	59	1	59	2.78e-29	108
Msa0575600	MtrunA17_Chr5g0444631	67.925	53	17	0	112	164	4	56	2.96e-18	77.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0575600	AT3G20800.1	59.032	310	119	2	6	310	5	311	5.10e-120	348
Msa0575600	AT5G12980.1	56.554	267	116	0	32	298	29	295	9.75e-103	304
Msa0575600	AT2G32550.3	32.900	231	143	6	67	287	42	270	7.57e-28	110
Msa0575600	AT2G32550.2	32.900	231	143	6	67	287	81	309	1.11e-27	110
Msa0575600	AT2G32550.1	32.500	200	128	4	104	298	29	226	7.27e-25	100

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Find 52 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCAGGAACCTTTAGTAGC+AGG	0.521492	4_1:+79520726	Msa0575600:CDS
AACCTTAAATACTCAAACTG+TGG	0.565441	4_1:-79518400	None:intergenic
AACTTTATAAGACGAGGTGT+AGG	0.638935	4_1:-79519500	None:intergenic
AAGAACCACTGCCCAAACGA+TGG	0.643808	4_1:+79515679	Msa0575600:CDS
AAGACCCAACAACATGGAAG+TGG	0.564589	4_1:+79520673	Msa0575600:CDS
AAGGTAACCCATGACTTCTT+TGG	0.326447	4_1:-79519104	None:intergenic
ACAAAGATGTTGTTCTTGAA+TGG	0.471978	4_1:+79518073	Msa0575600:CDS
AGACCCAACAACATGGAAGT+GGG	0.650091	4_1:+79520674	Msa0575600:CDS
AGAGTTTGCTAATGTAATGC+CGG	0.558847	4_1:-79519682	None:intergenic
AGGAAGGGATACAAATACAA+AGG	0.647299	4_1:-79518358	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATCGAAGATAGATTCGTA+AGG	-	chr4_1:79515781-79515800	None:intergenic	30.0%
TTGTTAGCCATTCTAAATAG+TGG	+	chr4_1:79515914-79515933	Msa0575600:intron	30.0%
TGTTAGCCATTCTAAATAGT+GGG	+	chr4_1:79515915-79515934	Msa0575600:intron	30.0%
TTCTATCCCACTATTTAGAA+TGG	-	chr4_1:79515924-79515943	None:intergenic	30.0%
TAGGATTATTATCTGACACA+TGG	+	chr4_1:79516071-79516090	Msa0575600:intron	30.0%
TTACCTATGGATTTAGTGAA+AGG	-	chr4_1:79516209-79516228	None:intergenic	30.0%
CGTGTATCAATTCAAGTATA+GGG	-	chr4_1:79516284-79516303	None:intergenic	30.0%
ACGTGTATCAATTCAAGTAT+AGG	-	chr4_1:79516285-79516304	None:intergenic	30.0%
AAGTATAAGAACACCCATTT+GGG	-	chr4_1:79516529-79516548	None:intergenic	30.0%
AAAGTATAAGAACACCCATT+TGG	-	chr4_1:79516530-79516549	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	79515599	79520797	79515599	ID=Msa0575600;Name=Msa0575600
chr4_1	mRNA	79515599	79520797	79515599	ID=Msa0575600-mRNA-1;Parent=Msa0575600;Name=Msa0575600-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0\|0\|0\|1\|1\|1\|9\|0\|323
chr4_1	exon	79515599	79515775	79515599	ID=Msa0575600-mRNA-1:exon:28378;Parent=Msa0575600-mRNA-1
chr4_1	exon	79517304	79517375	79517304	ID=Msa0575600-mRNA-1:exon:28379;Parent=Msa0575600-mRNA-1
chr4_1	exon	79517754	79517843	79517754	ID=Msa0575600-mRNA-1:exon:28380;Parent=Msa0575600-mRNA-1
chr4_1	exon	79518052	79518094	79518052	ID=Msa0575600-mRNA-1:exon:28381;Parent=Msa0575600-mRNA-1
chr4_1	exon	79518350	79518456	79518350	ID=Msa0575600-mRNA-1:exon:28382;Parent=Msa0575600-mRNA-1
chr4_1	exon	79519097	79519192	79519097	ID=Msa0575600-mRNA-1:exon:28383;Parent=Msa0575600-mRNA-1
chr4_1	exon	79519371	79519561	79519371	ID=Msa0575600-mRNA-1:exon:28384;Parent=Msa0575600-mRNA-1
chr4_1	exon	79519680	79519749	79519680	ID=Msa0575600-mRNA-1:exon:28385;Parent=Msa0575600-mRNA-1
chr4_1	exon	79520672	79520797	79520672	ID=Msa0575600-mRNA-1:exon:28386;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79515599	79515775	79515599	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79517304	79517375	79517304	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79517754	79517843	79517754	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79518052	79518094	79518052	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79518350	79518456	79518350	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79519097	79519192	79519097	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79519371	79519561	79519371	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79519680	79519749	79519680	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1
chr4_1	CDS	79520672	79520797	79520672	ID=Msa0575600-mRNA-1:cds;Parent=Msa0575600-mRNA-1

Gene Sequence

>Msa0575600

ATGGACAAAATTTCTCCTCATTCGCTCACAATGAAGAATGAATCTTCGTCTGTAGCTCCTCTTGGTGCTTCTACTCAATCAAGAACCACTGCCCAAACGATGGCTTGCATGGAACGCCTTGTTATTGAACTCAGCAATCCTGATCTTCGAGAAAACGCTCTTCGTGTGCTCTCCAAGAGGTATGAGTTATCTCCAGAACTTGCATCTTTATTATGGAATTCTTTTGGTATTATTGCAGTACTTTTACAGGAAATAACTTCAATATATTGTACTCTTTCACCACCGACTCTTACTCTAGCACAATCAACTCGAGTGTGTAACGTTCTTGCACTACTTCAGTGTGTGGCATCTCACTCTGAAACAAAGATGTTGTTCTTGAATGCTAGCATACCTTTGTATTTGTATCCCTTCCTTAAAACAAAAGACAAGTCACCACAGTTTGAGTATTTAAGGTTTGCAAGTCTTGGAGTCATTGGCGCCTTAGTGAAGGTTAACACCAAAGAAGTCATGGGTTACCTTATTTTAAGCGAGATAATTCCATTGTGCTTAAGCAACATGGAGATTGGAAATGAAATATCTAAAACTACTGCAACATTTATAATGCATAAAATTTTATTGGATGATGGTGGTTTGGCTTACGTTTGTGCTACAGCAGAGCGCTTTTTTGCAGTACGTCGTGTTTTGGACATGATGTTCGAAAGTCTTGACAAGCAACCTACACCTCGTCTTATAAAGTTTATAATTCCTTGCTATGCTCGTTTGTCTGATGATCGCTGGGCCGGCATTACATTAGCAAACTCTCTTCCTAGTGTGTTTAGAGACACCATTTTTCTTAATCAATTTCGTGAAGACCCAACAACATGGAAGTGGGTAAAGCACTTGCAGGAGAATGTGAGAAAGAACCAGGAACCTTTAGTAGCAGGTGGAGGAGAAAACAATGATATTATGGAGTCGTCAACATCAGGACAATGA

Protein sequence

>Msa0575600

MDKISPHSLTMKNESSSVAPLGASTQSRTTAQTMACMERLVIELSNPDLRENALRVLSKRYELSPELASLLWNSFGIIAVLLQEITSIYCTLSPPTLTLAQSTRVCNVLALLQCVASHSETKMLFLNASIPLYLYPFLKTKDKSPQFEYLRFASLGVIGALVKVNTKEVMGYLILSEIIPLCLSNMEIGNEISKTTATFIMHKILLDDGGLAYVCATAERFFAVRRVLDMMFESLDKQPTPRLIKFIIPCYARLSDDRWAGITLANSLPSVFRDTIFLNQFREDPTTWKWVKHLQENVRKNQEPLVAGGGENNDIMESSTSGQ*