AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0575790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575790 MtrunA17_Chr8g0393051 84.496 129 8 2 1 128 1 118 9.51e-74 215
Msa0575790 MtrunA17_Chr2g0285821 74.265 136 25 3 1 128 1 134 6.62e-66 196
Msa0575790 MtrunA17_Chr8g0376421 50.000 128 55 4 2 127 4 124 3.50e-30 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575790 AT1G30135.1 41.353 133 65 6 1 127 3 128 4.61e-22 85.1
Msa0575790 AT2G34600.1 39.535 129 73 5 1 127 20 145 1.90e-16 71.2

Find 41 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGATGTCACAGAGTTTC+AGG 0.175518 4_1:-79721392 Msa0575790:intron
GAAATGCTAACGTGTGTTAT+TGG 0.280506 4_1:-79721020 Msa0575790:three_prime_UTR
CTTCTTCAATGGATTGATCT+TGG 0.305845 4_1:+79720875 None:intergenic
TGCCAGCATTAAGATGGATT+TGG 0.344981 4_1:+79721257 None:intergenic
AGATGGAGATGATGGTTATA+AGG 0.359318 4_1:-79720931 Msa0575790:three_prime_UTR
GATAAGATTTACTTCTTCAA+TGG 0.365241 4_1:+79720864 None:intergenic
GGAAAGCCCAATGCAAAATC+AGG 0.374177 4_1:-79721455 Msa0575790:CDS
CAAATGAGGATCGCAATAAT+AGG 0.376605 4_1:-79720730 Msa0575790:three_prime_UTR
CAGAATCATAGAGAGGAAAA+AGG 0.376693 4_1:+79721640 None:intergenic
CATCTTCCCATCATAGAATA+TGG 0.389620 4_1:+79721420 None:intergenic
CCTTATCAATTGTATAGCCC+TGG 0.405940 4_1:-79721159 Msa0575790:CDS
TTTATTTGCCAGCATTAAGA+TGG 0.422633 4_1:+79721251 None:intergenic
TTCTCTTATCTTACATGTGG+TGG 0.433401 4_1:+79721598 None:intergenic
TTAAATTGCAAATAAATAGA+TGG 0.437016 4_1:-79720948 Msa0575790:three_prime_UTR
TAATGACTATCAAATTTAGA+AGG 0.445845 4_1:-79720534 Msa0575790:three_prime_UTR
CTTCAATGGATTGATCTTGG+AGG 0.455964 4_1:+79720878 None:intergenic
CCGCTGACCATATTCTATGA+TGG 0.457336 4_1:-79721427 Msa0575790:CDS
TTCATCAATCACAACATGCT+TGG 0.472054 4_1:+79720829 None:intergenic
AGTCATTAATAGTTGATGCT+TGG 0.478573 4_1:+79720549 None:intergenic
TCTACGGAAGAGAAAGAATA+GGG 0.480305 4_1:-79721100 Msa0575790:CDS
CAATTGTATAGCCCTGGGTC+TGG 0.487445 4_1:-79721153 Msa0575790:CDS
GATCTTTGCAACGGTTTCTA+CGG 0.498780 4_1:-79721116 Msa0575790:CDS
CTTATCAATTGTATAGCCCT+GGG 0.504415 4_1:-79721158 Msa0575790:CDS
TTCTACGGAAGAGAAAGAAT+AGG 0.504999 4_1:-79721101 Msa0575790:CDS
CGCTGACCATATTCTATGAT+GGG 0.509614 4_1:-79721426 Msa0575790:CDS
TAAGGAGGCTGCACTATTGT+TGG 0.509772 4_1:+79721177 None:intergenic
GGCCAAATCCATCTTAATGC+TGG 0.519970 4_1:-79721259 Msa0575790:CDS
GCAAAGATCTCTTCATTGAA+AGG 0.520559 4_1:+79721130 None:intergenic
GCTGTTCGCTCTGATCCTCT+TGG 0.521479 4_1:+79721201 None:intergenic
TTCATTGAAAGGCCAGACCC+AGG 0.532257 4_1:+79721141 None:intergenic
CCAGGGCTATACAATTGATA+AGG 0.533546 4_1:+79721159 None:intergenic
TGAGGATCGCAATAATAGGT+TGG 0.547830 4_1:-79720726 Msa0575790:three_prime_UTR
CAATGAAGAGATCTTTGCAA+CGG 0.550495 4_1:-79721125 Msa0575790:CDS
AAATAAATAGATGGAGATGA+TGG 0.553280 4_1:-79720939 Msa0575790:three_prime_UTR
AAAGGAAGAATTCAATGAGA+AGG 0.564203 4_1:-79720808 Msa0575790:three_prime_UTR
GATGATGGTTATAAGGAACA+TGG 0.598363 4_1:-79720924 Msa0575790:three_prime_UTR
GGGCTATACAATTGATAAGG+AGG 0.613096 4_1:+79721162 None:intergenic
TCATTGAAAGGCCAGACCCA+GGG 0.615225 4_1:+79721142 None:intergenic
AGCATGTTGTGATTGATGAA+AGG 0.640805 4_1:-79720826 Msa0575790:three_prime_UTR
CCATCATAGAATATGGTCAG+CGG 0.671722 4_1:+79721427 None:intergenic
TGATTGCCAGAATCATAGAG+AGG 0.688733 4_1:+79721633 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTCAAAAAAATAAATAAA+TGG - chr4_1:79720570-79720589 Msa0575790:three_prime_UTR 10.0%
!! CTTCAAAAAAATAAATAAAT+GGG - chr4_1:79720571-79720590 Msa0575790:three_prime_UTR 10.0%
!! TCTTCAAAAAAATAAATAAA+TGG - chr4_1:79720570-79720589 Msa0575790:three_prime_UTR 10.0%
!! CTTCAAAAAAATAAATAAAT+GGG - chr4_1:79720571-79720590 Msa0575790:three_prime_UTR 10.0%
!! AAGAAAATATAGTAAAAATG+AGG - chr4_1:79720186-79720205 Msa0575790:intron 15.0%
!! AGTATAACAAATTGAAAAAA+TGG + chr4_1:79720322-79720341 None:intergenic 15.0%
!!! TTTTTCAATTTGTTATACTA+TGG - chr4_1:79720323-79720342 Msa0575790:intron 15.0%
!!! TATGGTTTTCTTTTTATATT+TGG - chr4_1:79720341-79720360 Msa0575790:intron 15.0%
!!! ATGGTTTTCTTTTTATATTT+GGG - chr4_1:79720342-79720361 Msa0575790:intron 15.0%
!!! TCATTTTTTCATTTAGTTAT+TGG + chr4_1:79720540-79720559 None:intergenic 15.0%
!! TTAAATTGCAAATAAATAGA+TGG - chr4_1:79720924-79720943 Msa0575790:three_prime_UTR 15.0%
!!! TCTTTTTCTTATAAAGTAAA+GGG + chr4_1:79721550-79721569 None:intergenic 15.0%
!! GAAAAAAATTAAAGTTGTAT+TGG - chr4_1:79721639-79721658 Msa0575790:CDS 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr4_1:79721679-79721698 Msa0575790:exon 15.0%
!! AAGAAAATATAGTAAAAATG+AGG - chr4_1:79720186-79720205 Msa0575790:intron 15.0%
!! AGTATAACAAATTGAAAAAA+TGG + chr4_1:79720322-79720341 None:intergenic 15.0%
!!! TTTTTCAATTTGTTATACTA+TGG - chr4_1:79720323-79720342 Msa0575790:intron 15.0%
!!! TATGGTTTTCTTTTTATATT+TGG - chr4_1:79720341-79720360 Msa0575790:intron 15.0%
!!! ATGGTTTTCTTTTTATATTT+GGG - chr4_1:79720342-79720361 Msa0575790:intron 15.0%
!!! TCATTTTTTCATTTAGTTAT+TGG + chr4_1:79720540-79720559 None:intergenic 15.0%
!! TTAAATTGCAAATAAATAGA+TGG - chr4_1:79720924-79720943 Msa0575790:three_prime_UTR 15.0%
!!! TCTTTTTCTTATAAAGTAAA+GGG + chr4_1:79721550-79721569 None:intergenic 15.0%
!! GAAAAAAATTAAAGTTGTAT+TGG - chr4_1:79721639-79721658 Msa0575790:CDS 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr4_1:79721679-79721698 Msa0575790:exon 15.0%
!! AAAATATAGTAAAAATGAGG+AGG - chr4_1:79720189-79720208 Msa0575790:intron 20.0%
!! ACAAATTGAAAAAATGGAAA+TGG + chr4_1:79720316-79720335 None:intergenic 20.0%
!!! CAAATTTGTCTTTATCTTTT+AGG - chr4_1:79720830-79720849 Msa0575790:three_prime_UTR 20.0%
!! TAATGACTATCAAATTTAGA+AGG - chr4_1:79721220-79721239 Msa0575790:CDS 20.0%
!! GACTATATTCCAAAAAAAAT+TGG - chr4_1:79721264-79721283 Msa0575790:intron 20.0%
!!! TAAACTGTTCCAATTTTTTT+TGG + chr4_1:79721276-79721295 None:intergenic 20.0%
!! TAATGACTATCAAATTTAGA+AGG - chr4_1:79721220-79721239 Msa0575790:CDS 20.0%
!! GACTATATTCCAAAAAAAAT+TGG - chr4_1:79721264-79721283 Msa0575790:intron 20.0%
!!! GTCTTTTTCTTATAAAGTAA+AGG + chr4_1:79721551-79721570 None:intergenic 20.0%
!! AAAAGTTTGAGTATTTATTG+AGG - chr4_1:79721604-79721623 Msa0575790:intron 20.0%
!! AAAGTTTGAGTATTTATTGA+GGG - chr4_1:79721605-79721624 Msa0575790:intron 20.0%
!! AAAATATAGTAAAAATGAGG+AGG - chr4_1:79720189-79720208 Msa0575790:intron 20.0%
!! ACAAATTGAAAAAATGGAAA+TGG + chr4_1:79720316-79720335 None:intergenic 20.0%
!!! CAAATTTGTCTTTATCTTTT+AGG - chr4_1:79720830-79720849 Msa0575790:three_prime_UTR 20.0%
!! TAATGACTATCAAATTTAGA+AGG - chr4_1:79721220-79721239 Msa0575790:CDS 20.0%
!! GACTATATTCCAAAAAAAAT+TGG - chr4_1:79721264-79721283 Msa0575790:intron 20.0%
!!! TAAACTGTTCCAATTTTTTT+TGG + chr4_1:79721276-79721295 None:intergenic 20.0%
!! TAATGACTATCAAATTTAGA+AGG - chr4_1:79721220-79721239 Msa0575790:CDS 20.0%
!! GACTATATTCCAAAAAAAAT+TGG - chr4_1:79721264-79721283 Msa0575790:intron 20.0%
!!! GTCTTTTTCTTATAAAGTAA+AGG + chr4_1:79721551-79721570 None:intergenic 20.0%
!! AAAAGTTTGAGTATTTATTG+AGG - chr4_1:79721604-79721623 Msa0575790:intron 20.0%
!! AAAGTTTGAGTATTTATTGA+GGG - chr4_1:79721605-79721624 Msa0575790:intron 20.0%
! TTAATTTCTATCTCCATTGA+AGG - chr4_1:79720383-79720402 Msa0575790:intron 25.0%
! TAATTTCTATCTCCATTGAA+GGG - chr4_1:79720384-79720403 Msa0575790:intron 25.0%
!!! AAATTTGATTTTGCTAGTGA+TGG + chr4_1:79720817-79720836 None:intergenic 25.0%
! AAATAAATAGATGGAGATGA+TGG - chr4_1:79720933-79720952 Msa0575790:three_prime_UTR 25.0%
! GATAAGATTTACTTCTTCAA+TGG + chr4_1:79721011-79721030 None:intergenic 25.0%
!! AACTCATTTTCTTCTACTTT+TGG + chr4_1:79721111-79721130 None:intergenic 25.0%
!!! TACAAGACACCAATTTTTTT+TGG + chr4_1:79721394-79721413 None:intergenic 25.0%
! TTAATTTCTATCTCCATTGA+AGG - chr4_1:79720383-79720402 Msa0575790:intron 25.0%
! TAATTTCTATCTCCATTGAA+GGG - chr4_1:79720384-79720403 Msa0575790:intron 25.0%
!!! AAATTTGATTTTGCTAGTGA+TGG + chr4_1:79720817-79720836 None:intergenic 25.0%
! AAATAAATAGATGGAGATGA+TGG - chr4_1:79720933-79720952 Msa0575790:three_prime_UTR 25.0%
! GATAAGATTTACTTCTTCAA+TGG + chr4_1:79721011-79721030 None:intergenic 25.0%
!! AACTCATTTTCTTCTACTTT+TGG + chr4_1:79721111-79721130 None:intergenic 25.0%
!!! TACAAGACACCAATTTTTTT+TGG + chr4_1:79721394-79721413 None:intergenic 25.0%
AATTTCTATCTCCATTGAAG+GGG - chr4_1:79720385-79720404 Msa0575790:intron 30.0%
TTTATTTGCCAGCATTAAGA+TGG + chr4_1:79720624-79720643 None:intergenic 30.0%
AAAGGAAGAATTCAATGAGA+AGG - chr4_1:79721064-79721083 Msa0575790:exon 30.0%
!!! GTTTTCTAAGATGTCAAATG+AGG - chr4_1:79721128-79721147 Msa0575790:CDS 30.0%
! AGTCATTAATAGTTGATGCT+TGG + chr4_1:79721208-79721227 None:intergenic 30.0%
! AGTCATTAATAGTTGATGCT+TGG + chr4_1:79721208-79721227 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - chr4_1:79721433-79721452 Msa0575790:CDS 30.0%
TTGGCACACAGATTAAAAAA+TGG - chr4_1:79721458-79721477 Msa0575790:CDS 30.0%
AGACAAACATAAAGTTGCAT+TGG - chr4_1:79721567-79721586 Msa0575790:intron 30.0%
GTATTTATTGAGGGTAAAGT+TGG - chr4_1:79721614-79721633 Msa0575790:CDS 30.0%
AATTTCTATCTCCATTGAAG+GGG - chr4_1:79720385-79720404 Msa0575790:intron 30.0%
TTTATTTGCCAGCATTAAGA+TGG + chr4_1:79720624-79720643 None:intergenic 30.0%
AAAGGAAGAATTCAATGAGA+AGG - chr4_1:79721064-79721083 Msa0575790:exon 30.0%
!!! GTTTTCTAAGATGTCAAATG+AGG - chr4_1:79721128-79721147 Msa0575790:CDS 30.0%
! AGTCATTAATAGTTGATGCT+TGG + chr4_1:79721208-79721227 None:intergenic 30.0%
! AGTCATTAATAGTTGATGCT+TGG + chr4_1:79721208-79721227 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - chr4_1:79721433-79721452 Msa0575790:CDS 30.0%
TTGGCACACAGATTAAAAAA+TGG - chr4_1:79721458-79721477 Msa0575790:CDS 30.0%
AGACAAACATAAAGTTGCAT+TGG - chr4_1:79721567-79721586 Msa0575790:intron 30.0%
GTATTTATTGAGGGTAAAGT+TGG - chr4_1:79721614-79721633 Msa0575790:CDS 30.0%
CAGAATCATAGAGAGGAAAA+AGG + chr4_1:79720235-79720254 None:intergenic 35.0%
! CTTTTTCCTCTCTATGATTC+TGG - chr4_1:79720233-79720252 Msa0575790:intron 35.0%
TTCTCTTATCTTACATGTGG+TGG + chr4_1:79720277-79720296 None:intergenic 35.0%
GCATTCTCTTATCTTACATG+TGG + chr4_1:79720280-79720299 None:intergenic 35.0%
ATTTCTATCTCCATTGAAGG+GGG - chr4_1:79720386-79720405 Msa0575790:intron 35.0%
CATCTTCCCATCATAGAATA+TGG + chr4_1:79720455-79720474 None:intergenic 35.0%
CTTATCAATTGTATAGCCCT+GGG - chr4_1:79720714-79720733 Msa0575790:three_prime_UTR 35.0%
GCAAAGATCTCTTCATTGAA+AGG + chr4_1:79720745-79720764 None:intergenic 35.0%
CAATGAAGAGATCTTTGCAA+CGG - chr4_1:79720747-79720766 Msa0575790:three_prime_UTR 35.0%
TTCTACGGAAGAGAAAGAAT+AGG - chr4_1:79720771-79720790 Msa0575790:three_prime_UTR 35.0%
TCTACGGAAGAGAAAGAATA+GGG - chr4_1:79720772-79720791 Msa0575790:three_prime_UTR 35.0%
! TGATTTTGCTAGTGATGGTA+TGG + chr4_1:79720812-79720831 None:intergenic 35.0%
GAAATGCTAACGTGTGTTAT+TGG - chr4_1:79720852-79720871 Msa0575790:three_prime_UTR 35.0%
! AGATGGAGATGATGGTTATA+AGG - chr4_1:79720941-79720960 Msa0575790:three_prime_UTR 35.0%
! GATGATGGTTATAAGGAACA+TGG - chr4_1:79720948-79720967 Msa0575790:three_prime_UTR 35.0%
!! CTTCTTCAATGGATTGATCT+TGG + chr4_1:79721000-79721019 None:intergenic 35.0%
TTCATCAATCACAACATGCT+TGG + chr4_1:79721046-79721065 None:intergenic 35.0%
AGCATGTTGTGATTGATGAA+AGG - chr4_1:79721046-79721065 Msa0575790:three_prime_UTR 35.0%
CAAATGAGGATCGCAATAAT+AGG - chr4_1:79721142-79721161 Msa0575790:CDS 35.0%
!!! GACACTCATTTTGAAACGTA+GGG + chr4_1:79721428-79721447 None:intergenic 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr4_1:79721705-79721724 Msa0575790:five_prime_UTR 35.0%
CAGAATCATAGAGAGGAAAA+AGG + chr4_1:79720235-79720254 None:intergenic 35.0%
! CTTTTTCCTCTCTATGATTC+TGG - chr4_1:79720233-79720252 Msa0575790:intron 35.0%
TTCTCTTATCTTACATGTGG+TGG + chr4_1:79720277-79720296 None:intergenic 35.0%
GCATTCTCTTATCTTACATG+TGG + chr4_1:79720280-79720299 None:intergenic 35.0%
ATTTCTATCTCCATTGAAGG+GGG - chr4_1:79720386-79720405 Msa0575790:intron 35.0%
CATCTTCCCATCATAGAATA+TGG + chr4_1:79720455-79720474 None:intergenic 35.0%
CTTATCAATTGTATAGCCCT+GGG - chr4_1:79720714-79720733 Msa0575790:three_prime_UTR 35.0%
GCAAAGATCTCTTCATTGAA+AGG + chr4_1:79720745-79720764 None:intergenic 35.0%
CAATGAAGAGATCTTTGCAA+CGG - chr4_1:79720747-79720766 Msa0575790:three_prime_UTR 35.0%
TTCTACGGAAGAGAAAGAAT+AGG - chr4_1:79720771-79720790 Msa0575790:three_prime_UTR 35.0%
TCTACGGAAGAGAAAGAATA+GGG - chr4_1:79720772-79720791 Msa0575790:three_prime_UTR 35.0%
! TGATTTTGCTAGTGATGGTA+TGG + chr4_1:79720812-79720831 None:intergenic 35.0%
GAAATGCTAACGTGTGTTAT+TGG - chr4_1:79720852-79720871 Msa0575790:three_prime_UTR 35.0%
! AGATGGAGATGATGGTTATA+AGG - chr4_1:79720941-79720960 Msa0575790:three_prime_UTR 35.0%
! GATGATGGTTATAAGGAACA+TGG - chr4_1:79720948-79720967 Msa0575790:three_prime_UTR 35.0%
!! CTTCTTCAATGGATTGATCT+TGG + chr4_1:79721000-79721019 None:intergenic 35.0%
TTCATCAATCACAACATGCT+TGG + chr4_1:79721046-79721065 None:intergenic 35.0%
AGCATGTTGTGATTGATGAA+AGG - chr4_1:79721046-79721065 Msa0575790:three_prime_UTR 35.0%
CAAATGAGGATCGCAATAAT+AGG - chr4_1:79721142-79721161 Msa0575790:CDS 35.0%
!!! GACACTCATTTTGAAACGTA+GGG + chr4_1:79721428-79721447 None:intergenic 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr4_1:79721705-79721724 Msa0575790:five_prime_UTR 35.0%
TGATTGCCAGAATCATAGAG+AGG + chr4_1:79720242-79720261 None:intergenic 40.0%
TTTCTATCTCCATTGAAGGG+GGG - chr4_1:79720387-79720406 Msa0575790:intron 40.0%
! GCTGTTCCTGATTTTGCATT+GGG + chr4_1:79720426-79720445 None:intergenic 40.0%
CCATCATAGAATATGGTCAG+CGG + chr4_1:79720448-79720467 None:intergenic 40.0%
CGCTGACCATATTCTATGAT+GGG - chr4_1:79720446-79720465 Msa0575790:intron 40.0%
TACTGATGTCACAGAGTTTC+AGG - chr4_1:79720480-79720499 Msa0575790:three_prime_UTR 40.0%
GGTTAATGTGAATGTTGTGC+AGG - chr4_1:79720592-79720611 Msa0575790:intron 40.0%
! TGCCAGCATTAAGATGGATT+TGG + chr4_1:79720618-79720637 None:intergenic 40.0%
GAAAAAGTGAAGACACCAAG+AGG - chr4_1:79720656-79720675 Msa0575790:intron 40.0%
GGGCTATACAATTGATAAGG+AGG + chr4_1:79720713-79720732 None:intergenic 40.0%
CCAGGGCTATACAATTGATA+AGG + chr4_1:79720716-79720735 None:intergenic 40.0%
CCTTATCAATTGTATAGCCC+TGG - chr4_1:79720713-79720732 Msa0575790:three_prime_UTR 40.0%
GATCTTTGCAACGGTTTCTA+CGG - chr4_1:79720756-79720775 Msa0575790:three_prime_UTR 40.0%
!! CTTCAATGGATTGATCTTGG+AGG + chr4_1:79720997-79721016 None:intergenic 40.0%
TGAGGATCGCAATAATAGGT+TGG - chr4_1:79721146-79721165 Msa0575790:CDS 40.0%
!!! CGACACTCATTTTGAAACGT+AGG + chr4_1:79721429-79721448 None:intergenic 40.0%
!!! ATGAGTGTCGTTTTAGGTGT+TGG - chr4_1:79721439-79721458 Msa0575790:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+AGG - chr4_1:79721709-79721728 Msa0575790:five_prime_UTR 40.0%
TGATTGCCAGAATCATAGAG+AGG + chr4_1:79720242-79720261 None:intergenic 40.0%
TTTCTATCTCCATTGAAGGG+GGG - chr4_1:79720387-79720406 Msa0575790:intron 40.0%
! GCTGTTCCTGATTTTGCATT+GGG + chr4_1:79720426-79720445 None:intergenic 40.0%
CCATCATAGAATATGGTCAG+CGG + chr4_1:79720448-79720467 None:intergenic 40.0%
CGCTGACCATATTCTATGAT+GGG - chr4_1:79720446-79720465 Msa0575790:intron 40.0%
TACTGATGTCACAGAGTTTC+AGG - chr4_1:79720480-79720499 Msa0575790:three_prime_UTR 40.0%
GGTTAATGTGAATGTTGTGC+AGG - chr4_1:79720592-79720611 Msa0575790:intron 40.0%
! TGCCAGCATTAAGATGGATT+TGG + chr4_1:79720618-79720637 None:intergenic 40.0%
GAAAAAGTGAAGACACCAAG+AGG - chr4_1:79720656-79720675 Msa0575790:intron 40.0%
GGGCTATACAATTGATAAGG+AGG + chr4_1:79720713-79720732 None:intergenic 40.0%
CCAGGGCTATACAATTGATA+AGG + chr4_1:79720716-79720735 None:intergenic 40.0%
CCTTATCAATTGTATAGCCC+TGG - chr4_1:79720713-79720732 Msa0575790:three_prime_UTR 40.0%
GATCTTTGCAACGGTTTCTA+CGG - chr4_1:79720756-79720775 Msa0575790:three_prime_UTR 40.0%
!! CTTCAATGGATTGATCTTGG+AGG + chr4_1:79720997-79721016 None:intergenic 40.0%
TGAGGATCGCAATAATAGGT+TGG - chr4_1:79721146-79721165 Msa0575790:CDS 40.0%
!!! CGACACTCATTTTGAAACGT+AGG + chr4_1:79721429-79721448 None:intergenic 40.0%
!!! ATGAGTGTCGTTTTAGGTGT+TGG - chr4_1:79721439-79721458 Msa0575790:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+AGG - chr4_1:79721709-79721728 Msa0575790:five_prime_UTR 40.0%
GGAAAGCCCAATGCAAAATC+AGG - chr4_1:79720417-79720436 Msa0575790:intron 45.0%
! GGCTGTTCCTGATTTTGCAT+TGG + chr4_1:79720427-79720446 None:intergenic 45.0%
CCGCTGACCATATTCTATGA+TGG - chr4_1:79720445-79720464 Msa0575790:intron 45.0%
TTGGCCTAGTCAAGATCAGA+GGG + chr4_1:79720521-79720540 None:intergenic 45.0%
! ATTGGCCTAGTCAAGATCAG+AGG + chr4_1:79720522-79720541 None:intergenic 45.0%
GGCCAAATCCATCTTAATGC+TGG - chr4_1:79720613-79720632 Msa0575790:intron 45.0%
TAAGGAGGCTGCACTATTGT+TGG + chr4_1:79720698-79720717 None:intergenic 45.0%
GGAAAGCCCAATGCAAAATC+AGG - chr4_1:79720417-79720436 Msa0575790:intron 45.0%
! GGCTGTTCCTGATTTTGCAT+TGG + chr4_1:79720427-79720446 None:intergenic 45.0%
CCGCTGACCATATTCTATGA+TGG - chr4_1:79720445-79720464 Msa0575790:intron 45.0%
TTGGCCTAGTCAAGATCAGA+GGG + chr4_1:79720521-79720540 None:intergenic 45.0%
! ATTGGCCTAGTCAAGATCAG+AGG + chr4_1:79720522-79720541 None:intergenic 45.0%
GGCCAAATCCATCTTAATGC+TGG - chr4_1:79720613-79720632 Msa0575790:intron 45.0%
TAAGGAGGCTGCACTATTGT+TGG + chr4_1:79720698-79720717 None:intergenic 45.0%
TCAAGATCAGAGGGCGGTAA+TGG + chr4_1:79720512-79720531 None:intergenic 50.0%
CAATTGTATAGCCCTGGGTC+TGG - chr4_1:79720719-79720738 Msa0575790:three_prime_UTR 50.0%
TCATTGAAAGGCCAGACCCA+GGG + chr4_1:79720733-79720752 None:intergenic 50.0%
TTCATTGAAAGGCCAGACCC+AGG + chr4_1:79720734-79720753 None:intergenic 50.0%
TCAAGATCAGAGGGCGGTAA+TGG + chr4_1:79720512-79720531 None:intergenic 50.0%
CAATTGTATAGCCCTGGGTC+TGG - chr4_1:79720719-79720738 Msa0575790:three_prime_UTR 50.0%
TCATTGAAAGGCCAGACCCA+GGG + chr4_1:79720733-79720752 None:intergenic 50.0%
TTCATTGAAAGGCCAGACCC+AGG + chr4_1:79720734-79720753 None:intergenic 50.0%
CCTCTTCTACCCCCCTTCAA+TGG + chr4_1:79720399-79720418 None:intergenic 55.0%
CCATTGAAGGGGGGTAGAAG+AGG - chr4_1:79720396-79720415 Msa0575790:intron 55.0%
ACCGCCCTCTGATCTTGACT+AGG - chr4_1:79720514-79720533 Msa0575790:three_prime_UTR 55.0%
GCCTAGTCAAGATCAGAGGG+CGG + chr4_1:79720518-79720537 None:intergenic 55.0%
GCTGTTCGCTCTGATCCTCT+TGG + chr4_1:79720674-79720693 None:intergenic 55.0%
CCTCTTCTACCCCCCTTCAA+TGG + chr4_1:79720399-79720418 None:intergenic 55.0%
CCATTGAAGGGGGGTAGAAG+AGG - chr4_1:79720396-79720415 Msa0575790:intron 55.0%
ACCGCCCTCTGATCTTGACT+AGG - chr4_1:79720514-79720533 Msa0575790:three_prime_UTR 55.0%
GCCTAGTCAAGATCAGAGGG+CGG + chr4_1:79720518-79720537 None:intergenic 55.0%
GCTGTTCGCTCTGATCCTCT+TGG + chr4_1:79720674-79720693 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 79720141 79721753 79720141 ID=Msa0575790;Name=Msa0575790
chr4_1 mRNA 79720141 79721753 79720141 ID=Msa0575790-mRNA-1;Parent=Msa0575790;Name=Msa0575790-mRNA-1;_AED=0.04;_eAED=0.04;_QI=62|1|0.8|1|0.5|0.4|5|501|128
chr4_1 exon 79720141 79720163 79720141 ID=Msa0575790-mRNA-1:exon:28499;Parent=Msa0575790-mRNA-1
chr4_1 exon 79720475 79720593 79720475 ID=Msa0575790-mRNA-1:exon:28498;Parent=Msa0575790-mRNA-1
chr4_1 exon 79720712 79721280 79720712 ID=Msa0575790-mRNA-1:exon:28497;Parent=Msa0575790-mRNA-1
chr4_1 exon 79721393 79721489 79721393 ID=Msa0575790-mRNA-1:exon:28496;Parent=Msa0575790-mRNA-1
chr4_1 exon 79721612 79721753 79721612 ID=Msa0575790-mRNA-1:exon:28495;Parent=Msa0575790-mRNA-1
chr4_1 five_prime_UTR 79721692 79721753 79721692 ID=Msa0575790-mRNA-1:five_prime_utr;Parent=Msa0575790-mRNA-1
chr4_1 CDS 79721612 79721691 79721612 ID=Msa0575790-mRNA-1:cds;Parent=Msa0575790-mRNA-1
chr4_1 CDS 79721393 79721489 79721393 ID=Msa0575790-mRNA-1:cds;Parent=Msa0575790-mRNA-1
chr4_1 CDS 79721071 79721280 79721071 ID=Msa0575790-mRNA-1:cds;Parent=Msa0575790-mRNA-1
chr4_1 three_prime_UTR 79720712 79721070 79720712 ID=Msa0575790-mRNA-1:three_prime_utr;Parent=Msa0575790-mRNA-1
chr4_1 three_prime_UTR 79720475 79720593 79720475 ID=Msa0575790-mRNA-1:three_prime_utr;Parent=Msa0575790-mRNA-1
chr4_1 three_prime_UTR 79720141 79720163 79720141 ID=Msa0575790-mRNA-1:three_prime_utr;Parent=Msa0575790-mRNA-1
Gene Sequence

>Msa0575790

ATGAGGAGGAATTGTAACTTAGAACTCTGCCTTTTTCCTCTCTATGATTCTGGCAATCACAACAACAGCAACCACCACATGGGGGTAGAAGAGGAAAGCCCAATGCAAAATCAGGAACAGCCGCTGACCATATTCTATGATGGGAAGATGTGTCTTACTGATGTCACAGAGTTTCAGGCCAAATCCATCTTAATGCTGGCAAATAAAATTAAGTTGCAAGAAAAAGTGAAGACACCAAGAGGATCAGAGCGAACAGCACCAACAATAGTGCAGCCTCCTTATCAATTGTATAGCCCTGGGTCTGGCCTTTCAATGAAGAGATCTTTGCAACGGTTTCTACGGAAGAGAAAGAATAGGGTACAAGAAGCATCTCCATACCATCACTAG
Protein sequence

>Msa0575790

MRRNCNLELCLFPLYDSGNHNNSNHHMGVEEESPMQNQEQPLTIFYDGKMCLTDVTEFQAKSILMLANKIKLQEKVKTPRGSERTAPTIVQPPYQLYSPGSGLSMKRSLQRFLRKRKNRVQEASPYHH*