AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0575930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575930 MtrunA17_Chr4g0036071 73.913 69 18 0 1 69 334 402 9.32e-27 101
Msa0575930 MtrunA17_Chr2g0306581 83.673 49 8 0 15 63 1 49 7.69e-22 82.4
Msa0575930 MtrunA17_Chr1g0156051 67.797 59 19 0 1 59 361 419 1.35e-19 81.6
Msa0575930 MtrunA17_Chr2g0329551 58.824 68 28 0 2 69 196 263 1.81e-18 78.2
Msa0575930 MtrunA17_Chr6g0463571 67.273 55 18 0 11 65 135 189 3.26e-17 73.9
Msa0575930 MtrunA17_Chr5g0432701 63.333 60 22 0 10 69 142 201 4.24e-17 73.9
Msa0575930 MtrunA17_Chr3g0107491 63.333 60 22 0 10 69 160 219 5.11e-17 73.9
Msa0575930 MtrunA17_Chr3g0128781 56.667 60 26 0 10 69 91 150 1.56e-15 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0575930 AT2G21230.4 66.667 69 23 0 1 69 344 412 6.86e-23 91.3
Msa0575930 AT2G21230.1 66.667 69 23 0 1 69 344 412 6.86e-23 91.3
Msa0575930 AT2G21230.3 66.667 69 23 0 1 69 344 412 7.71e-23 91.3
Msa0575930 AT4G38900.2 70.769 65 19 0 5 69 372 436 8.32e-23 90.9
Msa0575930 AT4G38900.3 70.769 65 19 0 5 69 372 436 8.32e-23 90.9
Msa0575930 AT2G21230.2 67.647 68 22 0 2 69 345 412 5.97e-22 88.6
Msa0575930 AT4G38900.1 64.789 71 19 1 5 69 372 442 1.52e-20 84.7
Msa0575930 AT2G40620.1 66.667 60 20 0 10 69 131 190 6.72e-19 79.3
Msa0575930 AT2G31370.5 57.353 68 29 0 2 69 176 243 8.18e-18 76.6
Msa0575930 AT2G31370.2 57.353 68 29 0 2 69 176 243 9.48e-18 76.6
Msa0575930 AT2G31370.4 57.353 68 29 0 2 69 176 243 9.48e-18 76.6
Msa0575930 AT2G31370.1 57.353 68 29 0 2 69 176 243 9.48e-18 76.6
Msa0575930 AT2G31370.7 57.353 68 29 0 2 69 176 243 9.48e-18 76.6
Msa0575930 AT2G31370.3 57.353 68 29 0 2 69 176 243 9.48e-18 76.6
Msa0575930 AT2G31370.6 57.353 68 29 0 2 69 176 243 9.48e-18 76.6
Msa0575930 AT1G06070.1 55.882 68 30 0 2 69 185 252 7.95e-17 73.9
Msa0575930 AT1G06850.2 63.333 60 22 0 10 69 131 190 1.52e-16 71.6
Msa0575930 AT1G06850.1 63.333 60 22 0 10 69 131 190 2.60e-16 72.0
Msa0575930 AT1G43700.1 66.667 48 16 0 15 62 182 229 6.49e-15 68.2

Find 53 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGGTTATTTCAGGATTT+TGG 0.101232 4_1:-79906194 Msa0575930:intron
GGACCTTAGCAAACTGTATT+TGG 0.168656 4_1:-79905131 Msa0575930:three_prime_UTR
GCTTAAGATGCGATATATTT+TGG 0.170519 4_1:-79906140 Msa0575930:CDS
AATAAATTTATAAGGAGAAT+TGG 0.255363 4_1:-79905106 Msa0575930:three_prime_UTR
TGCTACCAACACTGCTTTCT+CGG 0.291207 4_1:+79905999 None:intergenic
ATTTGGAAAATAAATTTATA+AGG 0.294569 4_1:-79905114 Msa0575930:three_prime_UTR
TTCGGAGTTGAAGAAAATTA+TGG 0.328298 4_1:-79906342 Msa0575930:CDS
AAATTGTTAAAGAGCTTTGT+AGG 0.344037 4_1:-79905152 Msa0575930:three_prime_UTR
GGACTGTAATCTTGAACTTC+TGG 0.344694 4_1:+79905827 None:intergenic
AACACTGCTTTCTCGGTTCT+AGG 0.357024 4_1:+79906006 None:intergenic
GCAACGGCACAACGAATTCT+TGG 0.368486 4_1:+79905887 None:intergenic
GAGGTGTCATATTCTGAAAT+TGG 0.368658 4_1:+79906068 None:intergenic
ATCTAATGATTATAATCAAC+TGG 0.374871 4_1:+79905216 None:intergenic
ATAAATTTATAAGGAGAATT+GGG 0.377114 4_1:-79905105 Msa0575930:three_prime_UTR
TTTCTGAAATGCCTTCAGTT+TGG 0.388967 4_1:-79905014 Msa0575930:three_prime_UTR
AAAATAATACCTGGCCTTTG+TGG 0.394261 4_1:+79905788 None:intergenic
ATTGAAGGTCTTGTTGTGTT+TGG 0.431019 4_1:+79905312 None:intergenic
GTTCAGACTCTGAATACTAA+AGG 0.439473 4_1:-79906103 Msa0575930:CDS
TTGTGGAACCAAGCTCCATA+TGG 0.454668 4_1:+79905805 None:intergenic
CTATTCAGTTCCTTTGTGAA+TGG 0.458037 4_1:-79905345 Msa0575930:three_prime_UTR
AATAGATGCAGTGCTCTAGA+AGG 0.460695 4_1:+79905363 None:intergenic
GAAAGTTGTTGAGATTGTTA+TGG 0.462676 4_1:-79906312 Msa0575930:CDS
TCAGTTCCTTTGTGAATGGC+AGG 0.462751 4_1:-79905341 Msa0575930:three_prime_UTR
ATATCGCATCTTAAGCTCAA+TGG 0.469206 4_1:+79906147 None:intergenic
TTCAAGATTACAGTCCCATA+TGG 0.472759 4_1:-79905820 Msa0575930:three_prime_UTR
CAGCACCTCTTAGCTCGCTT+CGG 0.474909 4_1:+79906284 None:intergenic
GAATTCGTTGTGCCGTTGCT+AGG 0.480369 4_1:-79905883 Msa0575930:three_prime_UTR
TTAGACTGAGAAGAATGCTT+CGG 0.481879 4_1:-79906360 Msa0575930:intron
TTCAGACTCTGAATACTAAA+GGG 0.482971 4_1:-79906102 Msa0575930:CDS
AAATTGTGTAAAGAACAATA+TGG 0.484537 4_1:+79905037 None:intergenic
ACAACAAGACCTTCAATTGA+AGG 0.487972 4_1:-79905306 Msa0575930:three_prime_UTR
CTATGTGTAGGATGGAGGTT+TGG 0.488964 4_1:-79905460 Msa0575930:intron
GCTCTAGAAGGTTGTGCATA+GGG 0.490885 4_1:+79905375 None:intergenic
TTCCACATGTTATATACAGT+TGG 0.506432 4_1:+79905256 None:intergenic
TTACAGTCCCATATGGAGCT+TGG 0.523649 4_1:-79905813 Msa0575930:three_prime_UTR
TTTCCAAATACAGTTTGCTA+AGG 0.524653 4_1:+79905128 None:intergenic
TGTGGAACCAAGCTCCATAT+GGG 0.530718 4_1:+79905806 None:intergenic
TGCTCTAGAAGGTTGTGCAT+AGG 0.543792 4_1:+79905374 None:intergenic
AGGTTTGGCAACTTTGAGCA+TGG 0.546338 4_1:-79905445 Msa0575930:three_prime_UTR
ATGTGAGCTGCTCCTCGTAA+AGG 0.555373 4_1:+79905402 None:intergenic
CATATCTATGTGTAGGATGG+AGG 0.564335 4_1:-79905465 Msa0575930:intron
TAGAACCGAGAAAGCAGTGT+TGG 0.572911 4_1:-79906004 Msa0575930:intron
AGCTTGGTTCCACAAAGGCC+AGG 0.580658 4_1:-79905797 Msa0575930:intron
CTGATCCGAAGCGAGCTAAG+AGG 0.581733 4_1:-79906289 Msa0575930:intron
TGAAGCAAACTCAGCAAGAG+AGG 0.599458 4_1:+79906049 None:intergenic
TGCCAACTGTATATAACATG+TGG 0.599624 4_1:-79905258 Msa0575930:three_prime_UTR
CGAAAACTGATCCAAACTGA+AGG 0.608245 4_1:+79905003 None:intergenic
TGAGCTGCTCCTCGTAAAGG+TGG 0.621017 4_1:+79905405 None:intergenic
CAATCTCCTGCCATTCACAA+AGG 0.622463 4_1:+79905335 None:intergenic
TAACTTTCGCCACCTTTACG+AGG 0.625121 4_1:-79905414 Msa0575930:three_prime_UTR
TTTACGAGGAGCAGCTCACA+TGG 0.634002 4_1:-79905400 Msa0575930:three_prime_UTR
TATGGAGCTTGGTTCCACAA+AGG 0.680414 4_1:-79905802 Msa0575930:three_prime_UTR
AGTGCTCGAGAACCTAGCAA+CGG 0.707214 4_1:+79905871 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGGAAAATAAATTTATA+AGG - chr4_1:79906925-79906944 Msa0575930:intron 10.0%
!! ATCATAAAAAATAAAATGAA+TGG + chr4_1:79906973-79906992 None:intergenic 10.0%
!! ATTTGGAAAATAAATTTATA+AGG - chr4_1:79906925-79906944 Msa0575930:intron 10.0%
!! ATCATAAAAAATAAAATGAA+TGG + chr4_1:79906973-79906992 None:intergenic 10.0%
!! AAAAAAGACTACAAATTAAT+AGG + chr4_1:79904983-79905002 None:intergenic 15.0%
!! AATAAAAAATCTCAAGAAAA+AGG + chr4_1:79905050-79905069 None:intergenic 15.0%
!!! TTTTCTTGAGATTTTTTATT+TGG - chr4_1:79905050-79905069 Msa0575930:three_prime_UTR 15.0%
!!! GAAAAAAATCTACTTTTTTT+TGG + chr4_1:79905180-79905199 None:intergenic 15.0%
!! TACCATTATAAATGATAATT+TGG - chr4_1:79905217-79905236 Msa0575930:three_prime_UTR 15.0%
!! TTCCAAATTATCATTTATAA+TGG + chr4_1:79905222-79905241 None:intergenic 15.0%
!! ATTATAAATGATAATTTGGA+AGG - chr4_1:79905221-79905240 Msa0575930:three_prime_UTR 15.0%
!!! AATTATGTTTTGACTAAATT+AGG - chr4_1:79905298-79905317 Msa0575930:three_prime_UTR 15.0%
!! AAAAACATTTAAACTTCATA+AGG + chr4_1:79905393-79905412 None:intergenic 15.0%
!!! ATTTAACTTGGATATTTTTT+TGG - chr4_1:79905827-79905846 Msa0575930:three_prime_UTR 15.0%
!!! TTTAACTTGGATATTTTTTT+GGG - chr4_1:79905828-79905847 Msa0575930:three_prime_UTR 15.0%
!! AGAAATGATCAATAAAAATT+TGG + chr4_1:79906072-79906091 None:intergenic 15.0%
!! TGTTAAAAAAAAATAATACC+TGG + chr4_1:79906263-79906282 None:intergenic 15.0%
!! AATAAATTTATAAGGAGAAT+TGG - chr4_1:79906933-79906952 Msa0575930:intron 15.0%
!! ATAAATTTATAAGGAGAATT+GGG - chr4_1:79906934-79906953 Msa0575930:intron 15.0%
!! AAAAAAGACTACAAATTAAT+AGG + chr4_1:79904983-79905002 None:intergenic 15.0%
!! AATAAAAAATCTCAAGAAAA+AGG + chr4_1:79905050-79905069 None:intergenic 15.0%
!!! TTTTCTTGAGATTTTTTATT+TGG - chr4_1:79905050-79905069 Msa0575930:three_prime_UTR 15.0%
!!! GAAAAAAATCTACTTTTTTT+TGG + chr4_1:79905180-79905199 None:intergenic 15.0%
!! TACCATTATAAATGATAATT+TGG - chr4_1:79905217-79905236 Msa0575930:three_prime_UTR 15.0%
!! TTCCAAATTATCATTTATAA+TGG + chr4_1:79905222-79905241 None:intergenic 15.0%
!! ATTATAAATGATAATTTGGA+AGG - chr4_1:79905221-79905240 Msa0575930:three_prime_UTR 15.0%
!!! AATTATGTTTTGACTAAATT+AGG - chr4_1:79905298-79905317 Msa0575930:three_prime_UTR 15.0%
!! AAAAACATTTAAACTTCATA+AGG + chr4_1:79905393-79905412 None:intergenic 15.0%
!!! ATTTAACTTGGATATTTTTT+TGG - chr4_1:79905827-79905846 Msa0575930:three_prime_UTR 15.0%
!!! TTTAACTTGGATATTTTTTT+GGG - chr4_1:79905828-79905847 Msa0575930:three_prime_UTR 15.0%
!! AGAAATGATCAATAAAAATT+TGG + chr4_1:79906072-79906091 None:intergenic 15.0%
!! TGTTAAAAAAAAATAATACC+TGG + chr4_1:79906263-79906282 None:intergenic 15.0%
!! AATAAATTTATAAGGAGAAT+TGG - chr4_1:79906933-79906952 Msa0575930:intron 15.0%
!! ATAAATTTATAAGGAGAATT+GGG - chr4_1:79906934-79906953 Msa0575930:intron 15.0%
!!! TTGTGATTTATGTGTATTTT+AGG - chr4_1:79905411-79905430 Msa0575930:three_prime_UTR 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG - chr4_1:79905417-79905436 Msa0575930:three_prime_UTR 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG - chr4_1:79905797-79905816 Msa0575930:intron 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG - chr4_1:79905838-79905857 Msa0575930:three_prime_UTR 20.0%
!! GTGTTAAATTATTATGACAA+CGG - chr4_1:79906126-79906145 Msa0575930:CDS 20.0%
!! AATTGAAGGATGAAAAAAAA+TGG - chr4_1:79906747-79906766 Msa0575930:intron 20.0%
!! ATTGAAGGATGAAAAAAAAT+GGG - chr4_1:79906748-79906767 Msa0575930:intron 20.0%
!! ATCTAATGATTATAATCAAC+TGG + chr4_1:79906826-79906845 None:intergenic 20.0%
!! AAATTGTGTAAAGAACAATA+TGG + chr4_1:79907005-79907024 None:intergenic 20.0%
!!! ATTTTAAGAATGTTGTTGAT+TGG + chr4_1:79907069-79907088 None:intergenic 20.0%
!!! TTGTGATTTATGTGTATTTT+AGG - chr4_1:79905411-79905430 Msa0575930:three_prime_UTR 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG - chr4_1:79905417-79905436 Msa0575930:three_prime_UTR 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG - chr4_1:79905797-79905816 Msa0575930:intron 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG - chr4_1:79905838-79905857 Msa0575930:three_prime_UTR 20.0%
!! GTGTTAAATTATTATGACAA+CGG - chr4_1:79906126-79906145 Msa0575930:CDS 20.0%
!! AATTGAAGGATGAAAAAAAA+TGG - chr4_1:79906747-79906766 Msa0575930:intron 20.0%
!! ATTGAAGGATGAAAAAAAAT+GGG - chr4_1:79906748-79906767 Msa0575930:intron 20.0%
!! ATCTAATGATTATAATCAAC+TGG + chr4_1:79906826-79906845 None:intergenic 20.0%
!! AAATTGTGTAAAGAACAATA+TGG + chr4_1:79907005-79907024 None:intergenic 20.0%
!!! ATTTTAAGAATGTTGTTGAT+TGG + chr4_1:79907069-79907088 None:intergenic 20.0%
! CTAAATTACAATCTGTCATT+TGG - chr4_1:79905015-79905034 Msa0575930:three_prime_UTR 25.0%
!!! GGTATTGTTCTTTTTAACTA+TGG - chr4_1:79905071-79905090 Msa0575930:three_prime_UTR 25.0%
!!! AAATCTACTTTTTTTTGGCT+AGG + chr4_1:79905175-79905194 None:intergenic 25.0%
! ATAAATGATAATTTGGAAGG+AGG - chr4_1:79905224-79905243 Msa0575930:three_prime_UTR 25.0%
! ATATGTATCTTGACTCTATT+TGG - chr4_1:79905341-79905360 Msa0575930:three_prime_UTR 25.0%
!!! TATTTGGTCTTTGAGATATT+TGG - chr4_1:79905357-79905376 Msa0575930:three_prime_UTR 25.0%
!!! TTTACTGCTCTTATTTTACT+TGG - chr4_1:79905550-79905569 Msa0575930:intron 25.0%
! CATCAAAATTAGTTCAAATC+AGG + chr4_1:79905593-79905612 None:intergenic 25.0%
!!! TTGAACTAATTTTGATGTCT+TGG - chr4_1:79905596-79905615 Msa0575930:intron 25.0%
! TATGGATGGTTAATTTAACT+TGG - chr4_1:79905815-79905834 Msa0575930:three_prime_UTR 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG + chr4_1:79906292-79906311 None:intergenic 25.0%
! AAGTTCAGTATTCTATAGTA+TGG - chr4_1:79906312-79906331 Msa0575930:CDS 25.0%
!!! TTTTTTCATCCTTCAATTGA+AGG + chr4_1:79906745-79906764 None:intergenic 25.0%
!! AAATTGTTAAAGAGCTTTGT+AGG - chr4_1:79906887-79906906 Msa0575930:intron 25.0%
! CTAAATTACAATCTGTCATT+TGG - chr4_1:79905015-79905034 Msa0575930:three_prime_UTR 25.0%
!!! GGTATTGTTCTTTTTAACTA+TGG - chr4_1:79905071-79905090 Msa0575930:three_prime_UTR 25.0%
!!! AAATCTACTTTTTTTTGGCT+AGG + chr4_1:79905175-79905194 None:intergenic 25.0%
! ATAAATGATAATTTGGAAGG+AGG - chr4_1:79905224-79905243 Msa0575930:three_prime_UTR 25.0%
! ATATGTATCTTGACTCTATT+TGG - chr4_1:79905341-79905360 Msa0575930:three_prime_UTR 25.0%
!!! TATTTGGTCTTTGAGATATT+TGG - chr4_1:79905357-79905376 Msa0575930:three_prime_UTR 25.0%
!!! TTTACTGCTCTTATTTTACT+TGG - chr4_1:79905550-79905569 Msa0575930:intron 25.0%
! CATCAAAATTAGTTCAAATC+AGG + chr4_1:79905593-79905612 None:intergenic 25.0%
!!! TTGAACTAATTTTGATGTCT+TGG - chr4_1:79905596-79905615 Msa0575930:intron 25.0%
! TATGGATGGTTAATTTAACT+TGG - chr4_1:79905815-79905834 Msa0575930:three_prime_UTR 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG + chr4_1:79906292-79906311 None:intergenic 25.0%
! AAGTTCAGTATTCTATAGTA+TGG - chr4_1:79906312-79906331 Msa0575930:CDS 25.0%
!!! TTTTTTCATCCTTCAATTGA+AGG + chr4_1:79906745-79906764 None:intergenic 25.0%
!! AAATTGTTAAAGAGCTTTGT+AGG - chr4_1:79906887-79906906 Msa0575930:intron 25.0%
TTGTAATTTAGATGAGGCAT+GGG + chr4_1:79905006-79905025 None:intergenic 30.0%
ATTGTAATTTAGATGAGGCA+TGG + chr4_1:79905007-79905026 None:intergenic 30.0%
GACAGATTGTAATTTAGATG+AGG + chr4_1:79905012-79905031 None:intergenic 30.0%
!!! GCATTTTTTTGACTTCATGT+TGG - chr4_1:79905105-79905124 Msa0575930:three_prime_UTR 30.0%
ACAATGTGTGCTTACAATAT+GGG - chr4_1:79905487-79905506 Msa0575930:intron 30.0%
TTCGGAGTTGAAGAAAATTA+TGG - chr4_1:79905697-79905716 Msa0575930:intron 30.0%
GAAAGTTGTTGAGATTGTTA+TGG - chr4_1:79905727-79905746 Msa0575930:intron 30.0%
GTTAGTAATGTCAATATGGA+TGG - chr4_1:79905801-79905820 Msa0575930:three_prime_UTR 30.0%
!! TTTGGGTTATTTCAGGATTT+TGG - chr4_1:79905845-79905864 Msa0575930:three_prime_UTR 30.0%
! GCTTAAGATGCGATATATTT+TGG - chr4_1:79905899-79905918 Msa0575930:intron 30.0%
TTCAGACTCTGAATACTAAA+GGG - chr4_1:79905937-79905956 Msa0575930:intron 30.0%
! ATTAGCTTCTTAGTAATCAG+AGG + chr4_1:79906103-79906122 None:intergenic 30.0%
TTGTCACTGTTGTCAAATAA+AGG - chr4_1:79906379-79906398 Msa0575930:intron 30.0%
TTGTCAAATAAAGGCTATAG+TGG - chr4_1:79906388-79906407 Msa0575930:intron 30.0%
TTCCACATGTTATATACAGT+TGG + chr4_1:79906786-79906805 None:intergenic 30.0%
!! TTTCCAAATACAGTTTGCTA+AGG + chr4_1:79906914-79906933 None:intergenic 30.0%
TTGTAATTTAGATGAGGCAT+GGG + chr4_1:79905006-79905025 None:intergenic 30.0%
ATTGTAATTTAGATGAGGCA+TGG + chr4_1:79905007-79905026 None:intergenic 30.0%
GACAGATTGTAATTTAGATG+AGG + chr4_1:79905012-79905031 None:intergenic 30.0%
!!! GCATTTTTTTGACTTCATGT+TGG - chr4_1:79905105-79905124 Msa0575930:three_prime_UTR 30.0%
ACAATGTGTGCTTACAATAT+GGG - chr4_1:79905487-79905506 Msa0575930:intron 30.0%
TTCGGAGTTGAAGAAAATTA+TGG - chr4_1:79905697-79905716 Msa0575930:intron 30.0%
GAAAGTTGTTGAGATTGTTA+TGG - chr4_1:79905727-79905746 Msa0575930:intron 30.0%
GTTAGTAATGTCAATATGGA+TGG - chr4_1:79905801-79905820 Msa0575930:three_prime_UTR 30.0%
!! TTTGGGTTATTTCAGGATTT+TGG - chr4_1:79905845-79905864 Msa0575930:three_prime_UTR 30.0%
! GCTTAAGATGCGATATATTT+TGG - chr4_1:79905899-79905918 Msa0575930:intron 30.0%
TTCAGACTCTGAATACTAAA+GGG - chr4_1:79905937-79905956 Msa0575930:intron 30.0%
! ATTAGCTTCTTAGTAATCAG+AGG + chr4_1:79906103-79906122 None:intergenic 30.0%
TTGTCACTGTTGTCAAATAA+AGG - chr4_1:79906379-79906398 Msa0575930:intron 30.0%
TTGTCAAATAAAGGCTATAG+TGG - chr4_1:79906388-79906407 Msa0575930:intron 30.0%
TTCCACATGTTATATACAGT+TGG + chr4_1:79906786-79906805 None:intergenic 30.0%
!! TTTCCAAATACAGTTTGCTA+AGG + chr4_1:79906914-79906933 None:intergenic 30.0%
ACTCATTTACCTCTCATTGT+TGG + chr4_1:79905274-79905293 None:intergenic 35.0%
GACAATGTGTGCTTACAATA+TGG - chr4_1:79905486-79905505 Msa0575930:intron 35.0%
TTAGACTGAGAAGAATGCTT+CGG - chr4_1:79905679-79905698 Msa0575930:intron 35.0%
!!! ATTTCAGGATTTTGGCTAAC+AGG - chr4_1:79905853-79905872 Msa0575930:three_prime_UTR 35.0%
ATATCGCATCTTAAGCTCAA+TGG + chr4_1:79905895-79905914 None:intergenic 35.0%
!!! TATTTTGGAGCTAGAACACA+AGG - chr4_1:79905914-79905933 Msa0575930:intron 35.0%
GTTCAGACTCTGAATACTAA+AGG - chr4_1:79905936-79905955 Msa0575930:intron 35.0%
!! GAGGTGTCATATTCTGAAAT+TGG + chr4_1:79905974-79905993 None:intergenic 35.0%
TTCAAGATTACAGTCCCATA+TGG - chr4_1:79906219-79906238 Msa0575930:intron 35.0%
! AAAATAATACCTGGCCTTTG+TGG + chr4_1:79906254-79906273 None:intergenic 35.0%
TGCATTGCATATCTATGTGT+AGG - chr4_1:79906567-79906586 Msa0575930:intron 35.0%
TTGCATATCTATGTGTAGGA+TGG - chr4_1:79906571-79906590 Msa0575930:intron 35.0%
CTATTCAGTTCCTTTGTGAA+TGG - chr4_1:79906694-79906713 Msa0575930:intron 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG + chr4_1:79906730-79906749 None:intergenic 35.0%
ACAACAAGACCTTCAATTGA+AGG - chr4_1:79906733-79906752 Msa0575930:intron 35.0%
TGCCAACTGTATATAACATG+TGG - chr4_1:79906781-79906800 Msa0575930:intron 35.0%
!! TTTCTGAAATGCCTTCAGTT+TGG - chr4_1:79907025-79907044 Msa0575930:intron 35.0%
ACTCATTTACCTCTCATTGT+TGG + chr4_1:79905274-79905293 None:intergenic 35.0%
GACAATGTGTGCTTACAATA+TGG - chr4_1:79905486-79905505 Msa0575930:intron 35.0%
TTAGACTGAGAAGAATGCTT+CGG - chr4_1:79905679-79905698 Msa0575930:intron 35.0%
!!! ATTTCAGGATTTTGGCTAAC+AGG - chr4_1:79905853-79905872 Msa0575930:three_prime_UTR 35.0%
ATATCGCATCTTAAGCTCAA+TGG + chr4_1:79905895-79905914 None:intergenic 35.0%
!!! TATTTTGGAGCTAGAACACA+AGG - chr4_1:79905914-79905933 Msa0575930:intron 35.0%
GTTCAGACTCTGAATACTAA+AGG - chr4_1:79905936-79905955 Msa0575930:intron 35.0%
!! GAGGTGTCATATTCTGAAAT+TGG + chr4_1:79905974-79905993 None:intergenic 35.0%
TTCAAGATTACAGTCCCATA+TGG - chr4_1:79906219-79906238 Msa0575930:intron 35.0%
! AAAATAATACCTGGCCTTTG+TGG + chr4_1:79906254-79906273 None:intergenic 35.0%
TGCATTGCATATCTATGTGT+AGG - chr4_1:79906567-79906586 Msa0575930:intron 35.0%
TTGCATATCTATGTGTAGGA+TGG - chr4_1:79906571-79906590 Msa0575930:intron 35.0%
CTATTCAGTTCCTTTGTGAA+TGG - chr4_1:79906694-79906713 Msa0575930:intron 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG + chr4_1:79906730-79906749 None:intergenic 35.0%
ACAACAAGACCTTCAATTGA+AGG - chr4_1:79906733-79906752 Msa0575930:intron 35.0%
TGCCAACTGTATATAACATG+TGG - chr4_1:79906781-79906800 Msa0575930:intron 35.0%
!! TTTCTGAAATGCCTTCAGTT+TGG - chr4_1:79907025-79907044 Msa0575930:intron 35.0%
ATGTCTAAGCTGTAACTCCT+TGG - chr4_1:79905139-79905158 Msa0575930:three_prime_UTR 40.0%
TAAGGCTCCATTCATTGCTA+AGG - chr4_1:79905435-79905454 Msa0575930:three_prime_UTR 40.0%
!!! GATTTTGGCTAACAGGCAAT+CGG - chr4_1:79905860-79905879 Msa0575930:three_prime_UTR 40.0%
GGACTGTAATCTTGAACTTC+TGG + chr4_1:79906215-79906234 None:intergenic 40.0%
! GACAAAAACCACTGCTGATT+AGG + chr4_1:79906364-79906383 None:intergenic 40.0%
CCACGAATCGCAGAAAATAA+TGG + chr4_1:79906509-79906528 None:intergenic 40.0%
! CCATTATTTTCTGCGATTCG+TGG - chr4_1:79906506-79906525 Msa0575930:intron 40.0%
CATATCTATGTGTAGGATGG+AGG - chr4_1:79906574-79906593 Msa0575930:intron 40.0%
! AATAGATGCAGTGCTCTAGA+AGG + chr4_1:79906679-79906698 None:intergenic 40.0%
GGACCTTAGCAAACTGTATT+TGG - chr4_1:79906908-79906927 Msa0575930:intron 40.0%
CGAAAACTGATCCAAACTGA+AGG + chr4_1:79907039-79907058 None:intergenic 40.0%
ATGTCTAAGCTGTAACTCCT+TGG - chr4_1:79905139-79905158 Msa0575930:three_prime_UTR 40.0%
TAAGGCTCCATTCATTGCTA+AGG - chr4_1:79905435-79905454 Msa0575930:three_prime_UTR 40.0%
!!! GATTTTGGCTAACAGGCAAT+CGG - chr4_1:79905860-79905879 Msa0575930:three_prime_UTR 40.0%
GGACTGTAATCTTGAACTTC+TGG + chr4_1:79906215-79906234 None:intergenic 40.0%
! GACAAAAACCACTGCTGATT+AGG + chr4_1:79906364-79906383 None:intergenic 40.0%
CCACGAATCGCAGAAAATAA+TGG + chr4_1:79906509-79906528 None:intergenic 40.0%
! CCATTATTTTCTGCGATTCG+TGG - chr4_1:79906506-79906525 Msa0575930:intron 40.0%
CATATCTATGTGTAGGATGG+AGG - chr4_1:79906574-79906593 Msa0575930:intron 40.0%
! AATAGATGCAGTGCTCTAGA+AGG + chr4_1:79906679-79906698 None:intergenic 40.0%
GGACCTTAGCAAACTGTATT+TGG - chr4_1:79906908-79906927 Msa0575930:intron 40.0%
CGAAAACTGATCCAAACTGA+AGG + chr4_1:79907039-79907058 None:intergenic 40.0%
GGCTAGGTTAAAAGATGCCA+AGG + chr4_1:79905159-79905178 None:intergenic 45.0%
ACCTCTCATTGTTGGTGTGA+GGG + chr4_1:79905266-79905285 None:intergenic 45.0%
TACCTCTCATTGTTGGTGTG+AGG + chr4_1:79905267-79905286 None:intergenic 45.0%
CTCCATTCATTGCTAAGGCT+GGG - chr4_1:79905440-79905459 Msa0575930:three_prime_UTR 45.0%
TTCCCAGCCTTAGCAATGAA+TGG + chr4_1:79905445-79905464 None:intergenic 45.0%
TTGGACTGAGAAGACTGCTT+CGG - chr4_1:79905615-79905634 Msa0575930:intron 45.0%
TGAAGCAAACTCAGCAAGAG+AGG + chr4_1:79905993-79906012 None:intergenic 45.0%
AACACTGCTTTCTCGGTTCT+AGG + chr4_1:79906036-79906055 None:intergenic 45.0%
TAGAACCGAGAAAGCAGTGT+TGG - chr4_1:79906035-79906054 Msa0575930:CDS 45.0%
TGCTACCAACACTGCTTTCT+CGG + chr4_1:79906043-79906062 None:intergenic 45.0%
TTACAGTCCCATATGGAGCT+TGG - chr4_1:79906226-79906245 Msa0575930:intron 45.0%
TGTGGAACCAAGCTCCATAT+GGG + chr4_1:79906236-79906255 None:intergenic 45.0%
TTGTGGAACCAAGCTCCATA+TGG + chr4_1:79906237-79906256 None:intergenic 45.0%
TATGGAGCTTGGTTCCACAA+AGG - chr4_1:79906237-79906256 Msa0575930:intron 45.0%
! CCACTGCTGATTAGGAAAAC+TGG + chr4_1:79906356-79906375 None:intergenic 45.0%
! CCAGTTTTCCTAATCAGCAG+TGG - chr4_1:79906353-79906372 Msa0575930:CDS 45.0%
CTATGTGTAGGATGGAGGTT+TGG - chr4_1:79906579-79906598 Msa0575930:intron 45.0%
AGGTTTGGCAACTTTGAGCA+TGG - chr4_1:79906594-79906613 Msa0575930:intron 45.0%
TAACTTTCGCCACCTTTACG+AGG - chr4_1:79906625-79906644 Msa0575930:intron 45.0%
!! GCTCTAGAAGGTTGTGCATA+GGG + chr4_1:79906667-79906686 None:intergenic 45.0%
!! TGCTCTAGAAGGTTGTGCAT+AGG + chr4_1:79906668-79906687 None:intergenic 45.0%
TCAGTTCCTTTGTGAATGGC+AGG - chr4_1:79906698-79906717 Msa0575930:intron 45.0%
CAATCTCCTGCCATTCACAA+AGG + chr4_1:79906707-79906726 None:intergenic 45.0%
GGCTAGGTTAAAAGATGCCA+AGG + chr4_1:79905159-79905178 None:intergenic 45.0%
ACCTCTCATTGTTGGTGTGA+GGG + chr4_1:79905266-79905285 None:intergenic 45.0%
TACCTCTCATTGTTGGTGTG+AGG + chr4_1:79905267-79905286 None:intergenic 45.0%
CTCCATTCATTGCTAAGGCT+GGG - chr4_1:79905440-79905459 Msa0575930:three_prime_UTR 45.0%
TTCCCAGCCTTAGCAATGAA+TGG + chr4_1:79905445-79905464 None:intergenic 45.0%
TTGGACTGAGAAGACTGCTT+CGG - chr4_1:79905615-79905634 Msa0575930:intron 45.0%
TGAAGCAAACTCAGCAAGAG+AGG + chr4_1:79905993-79906012 None:intergenic 45.0%
AACACTGCTTTCTCGGTTCT+AGG + chr4_1:79906036-79906055 None:intergenic 45.0%
TAGAACCGAGAAAGCAGTGT+TGG - chr4_1:79906035-79906054 Msa0575930:CDS 45.0%
TGCTACCAACACTGCTTTCT+CGG + chr4_1:79906043-79906062 None:intergenic 45.0%
TTACAGTCCCATATGGAGCT+TGG - chr4_1:79906226-79906245 Msa0575930:intron 45.0%
TGTGGAACCAAGCTCCATAT+GGG + chr4_1:79906236-79906255 None:intergenic 45.0%
TTGTGGAACCAAGCTCCATA+TGG + chr4_1:79906237-79906256 None:intergenic 45.0%
TATGGAGCTTGGTTCCACAA+AGG - chr4_1:79906237-79906256 Msa0575930:intron 45.0%
! CCACTGCTGATTAGGAAAAC+TGG + chr4_1:79906356-79906375 None:intergenic 45.0%
! CCAGTTTTCCTAATCAGCAG+TGG - chr4_1:79906353-79906372 Msa0575930:CDS 45.0%
CTATGTGTAGGATGGAGGTT+TGG - chr4_1:79906579-79906598 Msa0575930:intron 45.0%
AGGTTTGGCAACTTTGAGCA+TGG - chr4_1:79906594-79906613 Msa0575930:intron 45.0%
TAACTTTCGCCACCTTTACG+AGG - chr4_1:79906625-79906644 Msa0575930:intron 45.0%
!! GCTCTAGAAGGTTGTGCATA+GGG + chr4_1:79906667-79906686 None:intergenic 45.0%
!! TGCTCTAGAAGGTTGTGCAT+AGG + chr4_1:79906668-79906687 None:intergenic 45.0%
TCAGTTCCTTTGTGAATGGC+AGG - chr4_1:79906698-79906717 Msa0575930:intron 45.0%
CAATCTCCTGCCATTCACAA+AGG + chr4_1:79906707-79906726 None:intergenic 45.0%
!! TTGGTGTGAGGGTGCTACAT+TGG + chr4_1:79905255-79905274 None:intergenic 50.0%
ACCCTCACACCAACAATGAG+AGG - chr4_1:79905262-79905281 Msa0575930:three_prime_UTR 50.0%
GCTCCATTCATTGCTAAGGC+TGG - chr4_1:79905439-79905458 Msa0575930:three_prime_UTR 50.0%
TGCTAAGGCTGGGAAGTTGA+AGG - chr4_1:79905450-79905469 Msa0575930:three_prime_UTR 50.0%
TAAGGCTGGGAAGTTGAAGG+AGG - chr4_1:79905453-79905472 Msa0575930:three_prime_UTR 50.0%
AAGACTGCTTCGGAGGAAGA+AGG - chr4_1:79905625-79905644 Msa0575930:intron 50.0%
GCAACGGCACAACGAATTCT+TGG + chr4_1:79906155-79906174 None:intergenic 50.0%
! GAATTCGTTGTGCCGTTGCT+AGG - chr4_1:79906156-79906175 Msa0575930:CDS 50.0%
AGTGCTCGAGAACCTAGCAA+CGG + chr4_1:79906171-79906190 None:intergenic 50.0%
TATAGTGGCGCTTTGCGTAG+TGG - chr4_1:79906403-79906422 Msa0575930:intron 50.0%
CGATTCGTGGTTGACATCAC+TGG - chr4_1:79906519-79906538 Msa0575930:intron 50.0%
ATGTGAGCTGCTCCTCGTAA+AGG + chr4_1:79906640-79906659 None:intergenic 50.0%
TTTACGAGGAGCAGCTCACA+TGG - chr4_1:79906639-79906658 Msa0575930:intron 50.0%
!! TTGGTGTGAGGGTGCTACAT+TGG + chr4_1:79905255-79905274 None:intergenic 50.0%
ACCCTCACACCAACAATGAG+AGG - chr4_1:79905262-79905281 Msa0575930:three_prime_UTR 50.0%
GCTCCATTCATTGCTAAGGC+TGG - chr4_1:79905439-79905458 Msa0575930:three_prime_UTR 50.0%
TGCTAAGGCTGGGAAGTTGA+AGG - chr4_1:79905450-79905469 Msa0575930:three_prime_UTR 50.0%
TAAGGCTGGGAAGTTGAAGG+AGG - chr4_1:79905453-79905472 Msa0575930:three_prime_UTR 50.0%
AAGACTGCTTCGGAGGAAGA+AGG - chr4_1:79905625-79905644 Msa0575930:intron 50.0%
GCAACGGCACAACGAATTCT+TGG + chr4_1:79906155-79906174 None:intergenic 50.0%
! GAATTCGTTGTGCCGTTGCT+AGG - chr4_1:79906156-79906175 Msa0575930:CDS 50.0%
AGTGCTCGAGAACCTAGCAA+CGG + chr4_1:79906171-79906190 None:intergenic 50.0%
TATAGTGGCGCTTTGCGTAG+TGG - chr4_1:79906403-79906422 Msa0575930:intron 50.0%
CGATTCGTGGTTGACATCAC+TGG - chr4_1:79906519-79906538 Msa0575930:intron 50.0%
ATGTGAGCTGCTCCTCGTAA+AGG + chr4_1:79906640-79906659 None:intergenic 50.0%
TTTACGAGGAGCAGCTCACA+TGG - chr4_1:79906639-79906658 Msa0575930:intron 50.0%
GACTGAGAAGACTGCTTCGG+AGG - chr4_1:79905618-79905637 Msa0575930:intron 55.0%
CTGATCCGAAGCGAGCTAAG+AGG - chr4_1:79905750-79905769 Msa0575930:intron 55.0%
CAGCACCTCTTAGCTCGCTT+CGG + chr4_1:79905758-79905777 None:intergenic 55.0%
AGCTTGGTTCCACAAAGGCC+AGG - chr4_1:79906242-79906261 Msa0575930:intron 55.0%
TGAGCTGCTCCTCGTAAAGG+TGG + chr4_1:79906637-79906656 None:intergenic 55.0%
GACTGAGAAGACTGCTTCGG+AGG - chr4_1:79905618-79905637 Msa0575930:intron 55.0%
CTGATCCGAAGCGAGCTAAG+AGG - chr4_1:79905750-79905769 Msa0575930:intron 55.0%
CAGCACCTCTTAGCTCGCTT+CGG + chr4_1:79905758-79905777 None:intergenic 55.0%
AGCTTGGTTCCACAAAGGCC+AGG - chr4_1:79906242-79906261 Msa0575930:intron 55.0%
TGAGCTGCTCCTCGTAAAGG+TGG + chr4_1:79906637-79906656 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 79904939 79907122 79904939 ID=Msa0575930;Name=Msa0575930
chr4_1 mRNA 79904939 79907122 79904939 ID=Msa0575930-mRNA-1;Parent=Msa0575930;Name=Msa0575930-mRNA-1;_AED=0.30;_eAED=0.31;_QI=0|0.75|0.6|1|0.5|0.4|5|671|91
chr4_1 exon 79904939 79905472 79904939 ID=Msa0575930-mRNA-1:exon:28557;Parent=Msa0575930-mRNA-1
chr4_1 exon 79905798 79905915 79905798 ID=Msa0575930-mRNA-1:exon:28556;Parent=Msa0575930-mRNA-1
chr4_1 exon 79906005 79906201 79906005 ID=Msa0575930-mRNA-1:exon:28555;Parent=Msa0575930-mRNA-1
chr4_1 exon 79906290 79906378 79906290 ID=Msa0575930-mRNA-1:exon:28554;Parent=Msa0575930-mRNA-1
chr4_1 exon 79907114 79907122 79907114 ID=Msa0575930-mRNA-1:exon:28553;Parent=Msa0575930-mRNA-1
chr4_1 CDS 79907114 79907122 79907114 ID=Msa0575930-mRNA-1:cds;Parent=Msa0575930-mRNA-1
chr4_1 CDS 79906290 79906378 79906290 ID=Msa0575930-mRNA-1:cds;Parent=Msa0575930-mRNA-1
chr4_1 CDS 79906024 79906201 79906024 ID=Msa0575930-mRNA-1:cds;Parent=Msa0575930-mRNA-1
chr4_1 three_prime_UTR 79906005 79906023 79906005 ID=Msa0575930-mRNA-1:three_prime_utr;Parent=Msa0575930-mRNA-1
chr4_1 three_prime_UTR 79905798 79905915 79905798 ID=Msa0575930-mRNA-1:three_prime_utr;Parent=Msa0575930-mRNA-1
chr4_1 three_prime_UTR 79904939 79905472 79904939 ID=Msa0575930-mRNA-1:three_prime_utr;Parent=Msa0575930-mRNA-1
Gene Sequence

>Msa0575930

ATGGGACAAACTGAGAAGAATGCTTCGGAGTTGAAGAAAATTATGGCTAATGAGAAAGTTGTTGAGATTGTTATGGCTGATCCGAAGCGAGCTAAGAGGATTTTGGCTAACAGGCAATCGGCTGCGCGTTCCATTGAGCTTAAGATGCGATATATTTTGGAGCTAGAACACAAGGTTCAGACTCTGAATACTAAAGGGTTGTTTCTTCTCCAATTTCAGAATATGACACCTCTCTTGCTGAGTTTGCTTCAGCAATTGTTGAGTTATGAAGCCTAG
Protein sequence

>Msa0575930

MGQTEKNASELKKIMANEKVVEIVMADPKRAKRILANRQSAARSIELKMRYILELEHKVQTLNTKGLFLLQFQNMTPLLLSLLQQLLSYEA*