AlfalfaGEDB | Msa0576670

M. sativa cultivar zhongmu-4 / Msa0576670

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576670	AET05311.1	86.611	478	44	4	11	470	1	476	0.0	830

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576670	sp\|Q8L3W1\|VRN1_ARATH	30.861	337	172	14	49	327	6	339	5.56e-34	133

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576670	G7L911	86.611	478	44	4	11	470	1	476	0.0	830

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TFs/TRs:

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Gene ID	Type	Classification
Msa0576670	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576670	MtrunA17_Chr8g0390841	82.635	501	44	5	11	470	1	499	0.0	817
Msa0576670	MtrunA17_Chr1g0208771	65.256	449	125	7	43	470	1	439	0.0	577
Msa0576670	MtrunA17_Chr1g0163071	47.525	303	124	10	65	336	2	300	2.32e-78	246
Msa0576670	MtrunA17_Chr7g0233561	35.127	316	148	9	47	324	16	312	2.31e-51	177
Msa0576670	MtrunA17_Chr3g0131891	33.435	329	164	9	47	343	17	322	3.99e-43	155
Msa0576670	MtrunA17_Chr7g0233541	34.237	295	143	11	65	324	2	280	1.26e-40	147
Msa0576670	MtrunA17_Chr3g0103181	31.818	308	141	9	47	323	10	279	8.68e-39	142
Msa0576670	MtrunA17_Chr7g0233521	34.138	290	133	9	65	324	2	263	5.00e-36	134
Msa0576670	MtrunA17_Chr7g0233531	33.218	289	137	7	65	324	2	263	1.60e-35	133
Msa0576670	MtrunA17_Chr3g0103191	31.560	282	127	11	41	290	4	251	1.33e-33	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0576670	AT3G18990.1	30.861	337	172	14	49	327	6	339	5.65e-35	133
Msa0576670	AT3G18990.2	30.746	335	171	14	49	325	6	337	4.87e-34	132
Msa0576670	AT3G18960.2	36.538	104	60	1	48	145	29	132	1.08e-15	75.9
Msa0576670	AT3G18960.1	36.538	104	60	1	48	145	29	132	1.65e-15	75.9
Msa0576670	AT4G01580.1	36.538	104	60	1	48	145	29	132	2.95e-15	74.7
Msa0576670	AT1G49475.1	26.573	143	93	3	23	154	3	144	2.69e-13	68.9
Msa0576670	AT1G49480.3	34.821	112	53	5	234	327	115	224	8.20e-13	68.2
Msa0576670	AT1G49480.1	34.821	112	53	5	234	327	115	224	8.20e-13	68.2
Msa0576670	AT1G49480.2	34.821	112	53	5	234	327	115	224	8.20e-13	68.2
Msa0576670	AT3G18960.3	37.500	88	49	1	64	145	8	95	1.24e-11	63.5

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Find 74 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTATCATGTCACATAA+AGG	0.413575	4_1:+80724582	Msa0576670:CDS
AAACATTCCCTTCCACCGCA+AGG	0.615531	4_1:+80724418	Msa0576670:CDS
AAACCTGTGGCATGTAAGAT+TGG	0.435151	4_1:+80725262	Msa0576670:CDS
AACTTGGTTGACGGTTCGGA+TGG	0.514973	4_1:-80725293	None:intergenic
AAGTATTTGAAGGAAAAGCA+AGG	0.649480	4_1:+80724208	Msa0576670:intron
AATTTATTATACCTTTAACT+TGG	0.446187	4_1:-80723774	None:intergenic
ACACATTATTCTATAGACCA+TGG	0.617727	4_1:+80723484	Msa0576670:CDS
ACCTCAACAAATATTCTAGA+TGG	0.390301	4_1:+80722311	Msa0576670:CDS
ACTCAACTTGGTTGACGGTT+CGG	0.425773	4_1:-80725297	None:intergenic
ACTCCAACCTGTACTCAACT+TGG	0.532809	4_1:-80725309	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATAAGCTGTTTATCCAAAC+GGG	-	chr4_1:80720921-80720940	None:intergenic	30.0%
CTATAAGCTGTTTATCCAAA+CGG	-	chr4_1:80720922-80720941	None:intergenic	30.0%
GATAAACAGCTTATAGCATT+AGG	+	chr4_1:80720926-80720945	Msa0576670:intron	30.0%
CAAAATAAGTCAATCCAAAC+GGG	+	chr4_1:80721685-80721704	Msa0576670:intron	30.0%
GATTATTGAATATTCCCGTT+TGG	-	chr4_1:80721702-80721721	None:intergenic	30.0%
TTATAGGAAAACCTTAGGTT+TGG	-	chr4_1:80721958-80721977	None:intergenic	30.0%
ACCTCAACAAATATTCTAGA+TGG	+	chr4_1:80722311-80722330	Msa0576670:CDS	30.0%
AAGAGAAAGTCGTCATAAAA+TGG	+	chr4_1:80722782-80722801	Msa0576670:intron	30.0%
ATCATATTACCCTATTACCA+AGG	-	chr4_1:80723134-80723153	None:intergenic	30.0%
GCTTTCACTAAGAAATATAG+TGG	+	chr4_1:80723349-80723368	Msa0576670:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	80720643	80725370	80720643	ID=Msa0576670;Name=Msa0576670
chr4_1	mRNA	80720643	80725370	80720643	ID=Msa0576670-mRNA-1;Parent=Msa0576670;Name=Msa0576670-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0\|0\|0\|0.88\|0.87\|0.66\|9\|0\|470
chr4_1	exon	80720643	80720651	80720643	ID=Msa0576670-mRNA-1:exon:28942;Parent=Msa0576670-mRNA-1
chr4_1	exon	80722161	80722340	80722161	ID=Msa0576670-mRNA-1:exon:28943;Parent=Msa0576670-mRNA-1
chr4_1	exon	80723337	80723549	80723337	ID=Msa0576670-mRNA-1:exon:28944;Parent=Msa0576670-mRNA-1
chr4_1	exon	80723568	80723784	80723568	ID=Msa0576670-mRNA-1:exon:28945;Parent=Msa0576670-mRNA-1
chr4_1	exon	80723891	80724054	80723891	ID=Msa0576670-mRNA-1:exon:28946;Parent=Msa0576670-mRNA-1
chr4_1	exon	80724220	80724439	80724220	ID=Msa0576670-mRNA-1:exon:28947;Parent=Msa0576670-mRNA-1
chr4_1	exon	80724526	80724603	80724526	ID=Msa0576670-mRNA-1:exon:28948;Parent=Msa0576670-mRNA-1
chr4_1	exon	80724851	80725002	80724851	ID=Msa0576670-mRNA-1:exon:28949;Parent=Msa0576670-mRNA-1
chr4_1	exon	80725194	80725370	80725194	ID=Msa0576670-mRNA-1:exon:28950;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80720643	80720651	80720643	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80722161	80722340	80722161	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80723337	80723549	80723337	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80723568	80723784	80723568	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80723891	80724054	80723891	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80724220	80724439	80724220	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80724526	80724603	80724526	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80724851	80725002	80724851	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1
chr4_1	CDS	80725194	80725370	80725194	ID=Msa0576670-mRNA-1:cds;Parent=Msa0576670-mRNA-1

Gene Sequence

>Msa0576670

ATGAATAACTCAATGATCATTAACCGACTTATGCCTCCCATTCACAGGAAAGGTGATCGATCGCTAAACATTAATACAAATCATCACAATTCTGAAGATTTATCAATGACTTGCAACCCAAGAAACTTGCATTCTGCTATCCGTTTCTTTAAGATTGTTACCTCAACAAATATTCTAGATGGAACCCTAAAAATTCCAAATGCTTTCACTAAGAAATATAGTGGTGATTTGTCAAACCCAATGTTTCTCAAGACTCCAGATGACAAAAAATGGGAAGTACATTTGACCAAAAAGGATGGTGATATTTGGATTCACAAGGGTTGGAATGAATTTGCTACACATTATTCTATAGACCATGGACACATGCTGATGTTTCAATATGAAAAAACCTCTCATTTTGAGAGTACTTTGGAAATTGAATATCACGTTGATAGTAATAATCAACATGAACAGAACAAACTTGTTGAAAATTTGGATGAAAAACCTACATGCAAGAAGACTAGATCAAAATCACAAATTTCAAGTCCTCAACCACATAAGAAATTGAGAATTGGCGCAAGTGAAGATGTTGGAACAAGCTCCAAATTGAAAAAAATTCCTAAACTTGTCCAAGTTAAAGAGGAGATTGAAGATACCACTAAATGCCTAAATGTGAAGCATGATCAAGAGCCGAATAATTCATCTTCAAAAATTGCTGAAGCTTTAAACAAAGCAAAAAACTACAAAACTAACAATCCCTTTTTCACTGTTGTCATGACGTATTCCTATGCTAATAAATACATGGAAAAGCAAGGCGTTATCACCCTTCAAGTCATGGATAATGAGAGAACTTGGAATGTTAAACATTGTGGGAGACATATTAGTACTGGATGGAAAAATTTTGCATTTGATAATAATTTGAAAGTGGGAGATGTTTGTCTTTTTGAGATGATCAAAAGCAATTCTTATGCTTTCAAAGTACTAATTTTTCGACTAGGCGAAAAACATTCCCTTCCACCGCAAGTTAATGGAGATGGAATTAATTGGGTTGAAACAGCAAGAATCCCTAGAGTTGAACATAAAACTATCATGTCACATAAAGGAAAGAAGGCAACTCAAAATTCACTGAATGCTAGTTCACGCTCTTTCAAAAGTTTTGAAGTTATGAAAGAATCCAATCAATTCACATCCACTCTGAAAAATCCACATTTCACTATCAAAGTGAAATCAAGTCATGCGGATGTTTACAAACCGCGTATACACAACTCGTTCTCCGAGAAGTACTTGTGTCACAAGAAGATTGTAACGTTACAGTTTAATAAAAACCTGTGGCATGTAAGATTGGCGAGCTGTCCATCCGAACCGTCAACCAAGTTGAGTACAGGTTGGAGTAAGTTTGTAGAGGAAAATAAATTGGAGGATGGAGATACA

Protein sequence

>Msa0576670

MNNSMIINRLMPPIHRKGDRSLNINTNHHNSEDLSMTCNPRNLHSAIRFFKIVTSTNILDGTLKIPNAFTKKYSGDLSNPMFLKTPDDKKWEVHLTKKDGDIWIHKGWNEFATHYSIDHGHMLMFQYEKTSHFESTLEIEYHVDSNNQHEQNKLVENLDEKPTCKKTRSKSQISSPQPHKKLRIGASEDVGTSSKLKKIPKLVQVKEEIEDTTKCLNVKHDQEPNNSSSKIAEALNKAKNYKTNNPFFTVVMTYSYANKYMEKQGVITLQVMDNERTWNVKHCGRHISTGWKNFAFDNNLKVGDVCLFEMIKSNSYAFKVLIFRLGEKHSLPPQVNGDGINWVETARIPRVEHKTIMSHKGKKATQNSLNASSRSFKSFEVMKESNQFTSTLKNPHFTIKVKSSHADVYKPRIHNSFSEKYLCHKKIVTLQFNKNLWHVRLASCPSEPSTKLSTGWSKFVEENKLEDGDT