AlfalfaGEDB | Msa0583000

M. sativa cultivar zhongmu-4 / Msa0583000

KEGG: -
GO: GO:0000278 GO:0000281 GO:0000910 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007049 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010324 GO:0010562 GO:0010604 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0022402 GO:0023014 GO:0023052 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032506 GO:0033554 GO:0033674 GO:0035556 GO:0036089 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051301 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0061024 GO:0061640 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0080090 GO:0090702 GO:0099024 GO:0099120 GO:0140096 GO:1901564 GO:1903047
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0583000	XP_015387578.1	89.474	133	7	1	1	126	1	133	2.41e-78	238

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0583000	sp\|Q9LJD8\|M3KE1_ARATH	87.500	128	9	1	1	121	1	128	5.41e-68	224

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0583000	MtrunA17_Chr4g0038501	94.531	128	0	1	1	121	1	128	2.60e-75	242
Msa0583000	MtrunA17_Chr4g0038091	92.188	128	3	1	1	121	1	128	6.64e-74	238
Msa0583000	MtrunA17_Chr1g0205931	38.793	116	56	3	19	121	54	167	1.37e-22	91.7
Msa0583000	MtrunA17_Chr7g0272841	33.621	116	62	2	19	121	67	180	1.45e-19	83.2
Msa0583000	MtrunA17_Chr8g0384301	34.646	127	71	3	5	121	284	408	7.03e-17	75.5
Msa0583000	MtrunA17_Chr5g0419441	38.393	112	56	3	19	123	238	343	6.52e-16	72.8
Msa0583000	MtrunA17_Chr1g0202831	32.308	130	66	4	2	121	403	520	1.66e-13	65.9
Msa0583000	MtrunA17_Chr5g0405421	28.682	129	81	3	10	128	89	216	5.49e-13	64.3
Msa0583000	MtrunA17_Chr5g0424581	33.028	109	59	4	23	121	128	232	8.38e-13	63.5
Msa0583000	MtrunA17_Chr5g0424651	33.028	109	59	4	23	121	324	428	9.16e-13	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0583000	AT3G13530.1	87.500	128	9	1	1	121	1	128	5.50e-69	224
Msa0583000	AT3G07980.2	86.719	128	10	1	1	121	1	128	3.07e-68	222
Msa0583000	AT3G07980.1	86.719	128	10	1	1	121	1	128	3.41e-68	222
Msa0583000	AT1G09000.1	40.179	112	52	3	23	121	72	181	3.30e-22	91.3
Msa0583000	AT1G09000.2	40.179	112	52	3	23	121	72	181	3.88e-22	90.9
Msa0583000	AT1G54960.1	37.931	116	57	3	19	121	67	180	3.68e-21	88.2
Msa0583000	AT1G54960.2	37.931	116	57	3	19	121	67	180	3.68e-21	88.2
Msa0583000	AT3G06030.1	35.345	116	60	2	19	121	67	180	5.53e-21	87.8
Msa0583000	AT1G69220.1	35.385	130	70	4	1	123	231	353	9.33e-17	75.5
Msa0583000	AT4G12020.1	36.607	112	57	4	20	121	1626	1733	4.22e-14	68.2

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Find 51 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGTGTGCTATTATAA+CGG	0.503563	4_2:-1344956	None:intergenic
AAAGGTTTGGACTTGGAGAA+TGG	0.441540	4_2:+1346097	Msa0583000:CDS
AACAGTTAAGCCTACAAACC+TGG	0.445198	4_2:-1348858	None:intergenic
AACGAAATCCTAAACGGCAG+TGG	0.574645	4_2:-1345087	None:intergenic
AACGGCGGTGGTGGCAGTGG+CGG	0.485336	4_2:-1345123	None:intergenic
AATCGGCGGCGACATGGATA+CGG	0.466925	4_2:+1345035	Msa0583000:five_prime_UTR
ACAGTTAAGCCTACAAACCT+GGG	0.610281	4_2:-1348857	None:intergenic
ACATGGATACGGAAATTTGA+GGG	0.567198	4_2:+1345046	Msa0583000:five_prime_UTR
AGATGCTGGGAGATGAGATT+GGG	0.503455	4_2:+1346050	Msa0583000:intron
AGGATAAACGAAATCCTAAA+CGG	0.502333	4_2:-1345093	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTGACTGAAGCAAAAATG+GGG	-	chr4_2:1344997-1345016	None:intergenic	30.0%
CTATTTGACTGAAGCAAAAA+TGG	-	chr4_2:1344999-1345018	None:intergenic	30.0%
AGGATAAACGAAATCCTAAA+CGG	-	chr4_2:1345096-1345115	None:intergenic	30.0%
ATTTGATTAGTGGAAGGTTT+AGG	+	chr4_2:1345307-1345326	Msa0583000:intron	30.0%
AAATGATTGTAGTAATGGTG+AGG	+	chr4_2:1345417-1345436	Msa0583000:intron	30.0%
CAGTACAATTGAAATAGAGA+TGG	+	chr4_2:1345581-1345600	Msa0583000:intron	30.0%
TACAATTGAAATAGAGATGG+TGG	+	chr4_2:1345584-1345603	Msa0583000:intron	30.0%
CAATGATTGTGTGATTAAAG+CGG	+	chr4_2:1346297-1346316	Msa0583000:intron	30.0%
AATGATTGTGTGATTAAAGC+GGG	+	chr4_2:1346298-1346317	Msa0583000:intron	30.0%
AATATCATCATTAGTGTCGT+AGG	+	chr4_2:1346669-1346688	Msa0583000:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	1344942	1348888	1344942	ID=Msa0583000;Name=Msa0583000
chr4_2	mRNA	1344942	1348888	1344942	ID=Msa0583000-mRNA-1;Parent=Msa0583000;Name=Msa0583000-mRNA-1;_AED=0.06;_eAED=0.06;_QI=211\|0.66\|0.75\|1\|1\|1\|4\|0\|128
chr4_2	exon	1344942	1345212	1344942	ID=Msa0583000-mRNA-1:exon:366;Parent=Msa0583000-mRNA-1
chr4_2	exon	1346052	1346186	1346052	ID=Msa0583000-mRNA-1:exon:367;Parent=Msa0583000-mRNA-1
chr4_2	exon	1348598	1348671	1348598	ID=Msa0583000-mRNA-1:exon:368;Parent=Msa0583000-mRNA-1
chr4_2	exon	1348771	1348888	1348771	ID=Msa0583000-mRNA-1:exon:369;Parent=Msa0583000-mRNA-1
chr4_2	five_prime_UTR	1344942	1345152	1344942	ID=Msa0583000-mRNA-1:five_prime_utr;Parent=Msa0583000-mRNA-1
chr4_2	CDS	1345153	1345212	1345153	ID=Msa0583000-mRNA-1:cds;Parent=Msa0583000-mRNA-1
chr4_2	CDS	1346052	1346186	1346052	ID=Msa0583000-mRNA-1:cds;Parent=Msa0583000-mRNA-1
chr4_2	CDS	1348598	1348671	1348598	ID=Msa0583000-mRNA-1:cds;Parent=Msa0583000-mRNA-1
chr4_2	CDS	1348771	1348888	1348771	ID=Msa0583000-mRNA-1:cds;Parent=Msa0583000-mRNA-1

Gene Sequence

>Msa0583000

ATGTCGCGTCAGTCTACTGGTTCCGCTTTCACCAAATCCAAAACTCTTGATAATAAATATATGCTGGGAGATGAGATTGGGAAAGGAGCTTATGGTCGAGTTTACAAAGGTTTGGACTTGGAGAATGGAGATTTTGTTGCAATTAAACAAGTTTCTTTGGAGAATATTGCTCAGGAGGATCTCAACATTATCATGAATTTGAATCACAAAAACATTGTAAAATATCTTGGATCTTTGAAGACAAAGAGTCACCTACACATAATTCTTGAGTATGTTGAGAATGGCTCACTTGCAAATATTATCAAGCCAAATAAATTTGGACCCTTTCCAGAATCGTTGGTTGCAGTTTATATTGCCCAGGTTTGTAGGCTTAACTGTTTGTGCTAA

Protein sequence

>Msa0583000

MSRQSTGSAFTKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNIIMNLNHKNIVKYLGSLKTKSHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYIAQVCRLNCLC*