AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0583860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0583860 MtrunA17_Chr1g0163231 70.455 308 38 2 1 275 1 288 7.88e-149 423
Msa0583860 MtrunA17_Chr1g0171611 65.605 314 37 4 1 276 1 281 6.56e-134 385
Msa0583860 MtrunA17_Chr1g0163181 40.909 418 174 10 44 397 20 428 2.75e-94 290
Msa0583860 MtrunA17_Chr1g0163201 42.184 403 155 10 44 397 18 391 4.07e-89 275
Msa0583860 MtrunA17_Chr1g0163251 42.515 334 129 11 25 302 3 329 7.69e-69 223
Msa0583860 MtrunA17_Chr1g0163261 41.509 318 103 8 1 268 1 285 1.82e-67 216
Msa0583860 MtrunA17_Chr1g0163241 57.241 145 55 4 27 167 7 148 3.26e-46 156
Msa0583860 MtrunA17_Chr1g0163111 42.466 219 107 7 184 402 33 232 1.50e-42 149
Msa0583860 MtrunA17_Chr1g0163151 39.720 214 100 5 184 397 36 220 4.40e-41 145
Msa0583860 MtrunA17_Chr1g0163191 63.000 100 37 0 44 143 20 119 1.09e-38 136
Msa0583860 MtrunA17_Chr1g0162151 39.910 223 86 9 181 403 38 212 2.92e-32 121
Msa0583860 MtrunA17_Chr1g0162161 41.892 148 65 4 42 174 1 142 3.36e-29 111
Msa0583860 MtrunA17_Chr6g0476171 31.203 266 148 7 137 397 9 244 7.10e-25 102
Msa0583860 MtrunA17_Chr1g0163121 41.791 134 67 4 42 165 8 140 3.51e-24 97.4
Msa0583860 MtrunA17_Chr1g0163081 46.078 102 54 1 42 143 3 103 6.87e-23 94.0
Msa0583860 MtrunA17_Chr1g0163101 41.667 132 66 4 45 169 33 160 7.18e-23 94.7
Msa0583860 MtrunA17_Chr8g0390841 23.529 510 271 18 2 399 16 518 1.27e-22 99.8
Msa0583860 MtrunA17_Chr1g0208771 23.179 453 257 11 38 399 6 458 1.54e-22 99.0
Msa0583860 MtrunA17_Chr1g0163131 41.176 153 79 5 253 396 154 304 3.39e-22 96.3
Msa0583860 MtrunA17_Chr1g0163131 36.667 150 83 5 36 176 27 173 5.47e-22 95.5
Msa0583860 MtrunA17_Chr1g0163141 42.574 101 55 2 40 140 8 105 4.65e-22 92.8
Msa0583860 MtrunA17_Chr7g0233561 28.873 284 142 9 41 264 27 310 5.03e-22 95.9
Msa0583860 MtrunA17_Chr6g0476181 38.931 131 73 3 45 174 2 126 7.75e-21 88.6
Msa0583860 MtrunA17_Chr7g0233521 28.464 267 137 8 45 264 2 261 5.74e-20 89.4
Msa0583860 MtrunA17_Chr3g0103181 25.357 280 158 8 27 264 8 278 1.23e-19 88.2
Msa0583860 MtrunA17_Chr7g0233531 28.044 271 133 8 45 264 2 261 3.96e-19 86.7
Msa0583860 MtrunA17_Chr7g0233541 27.972 286 131 10 45 264 2 278 1.73e-17 82.4
Msa0583860 MtrunA17_Chr3g0131891 26.394 269 147 8 45 264 35 301 1.80e-17 82.8
Msa0583860 MtrunA17_Chr3g0103151 32.168 143 88 3 27 166 8 144 2.88e-17 78.6
Msa0583860 MtrunA17_Chr5g0419201 33.577 137 85 2 45 175 32 168 5.89e-17 78.6
Msa0583860 MtrunA17_Chr3g0103171 25.564 266 153 9 41 264 2 264 8.50e-17 79.7
Msa0583860 MtrunA17_Chr6g0467351 36.641 131 76 3 44 171 30 156 4.96e-16 77.0
Msa0583860 MtrunA17_Chr1g0163091 50.588 85 38 3 184 268 41 121 5.11e-15 72.4
Msa0583860 MtrunA17_Chr7g0233571 43.373 83 45 1 45 125 26 108 8.46e-15 73.2
Msa0583860 MtrunA17_Chr3g0103191 34.167 120 76 2 44 160 25 144 9.55e-15 73.6
Msa0583860 MtrunA17_Chr1g0163161 35.385 130 69 5 57 174 1 127 2.03e-14 69.7
Msa0583860 MtrunA17_Chr1g0163221 25.256 293 158 11 45 282 27 313 8.47e-14 72.4
Msa0583860 MtrunA17_Chr4g0067431 23.077 286 150 9 44 264 30 310 2.83e-13 70.5
Msa0583860 MtrunA17_Chr3g0077451 23.715 253 148 8 41 270 8 238 3.21e-13 68.9
Msa0583860 MtrunA17_Chr1g0163071 37.349 83 50 1 45 125 2 84 1.23e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 67 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTGCAGATGACCCTTTA+AGG 0.106528 4_2:+2667301 None:intergenic
GTGCTCAACAGGAAATATTT+TGG 0.162791 4_2:-2668228 Msa0583860:CDS
AATATCTTAACCTTAAAATA+TGG 0.178478 4_2:+2668106 None:intergenic
TCTATTGTGATGATAATATT+TGG 0.191054 4_2:-2666959 Msa0583860:CDS
TGCAGGTTGGCATAAATTTA+AGG 0.202920 4_2:-2666217 Msa0583860:CDS
ATATTTGGTTTAAGAGAAAT+TGG 0.232188 4_2:-2668189 Msa0583860:CDS
AGAAATTTGTGGAGAAATAT+TGG 0.241294 4_2:-2668291 Msa0583860:CDS
TTTGGGAGTCTAAGAATTAC+TGG 0.267570 4_2:+2668253 None:intergenic
TATTTGAATGACAACCTTAA+AGG 0.276541 4_2:-2667315 Msa0583860:CDS
AGTCTAAGAATTACTGGATT+TGG 0.299817 4_2:+2668259 None:intergenic
ACAATAATCATTTATGAATA+AGG 0.308180 4_2:+2668333 None:intergenic
ATATTTCTCCACAAATTTCT+TGG 0.318034 4_2:+2668294 None:intergenic
ACTATCTTCAAGAACTCTTC+TGG 0.329140 4_2:+2668548 None:intergenic
TGGAGCGTGACGGTGATATT+TGG 0.333381 4_2:-2668204 Msa0583860:CDS
AACTTCACCTCCACTTTCAT+AGG 0.341509 4_2:+2667258 None:intergenic
ATTTCCTGTTGAGCACCATT+TGG 0.373513 4_2:+2668235 None:intergenic
ATTTGAATGACAACCTTAAA+GGG 0.391511 4_2:-2667314 Msa0583860:CDS
ACTCCCAAATGGTGCTCAAC+AGG 0.399322 4_2:-2668239 Msa0583860:CDS
TGGAAGAAAAGAGGCATTGA+AGG 0.400150 4_2:-2666939 Msa0583860:CDS
TATTTCTCCACAAATTTCTT+GGG 0.405795 4_2:+2668295 None:intergenic
TTTCCTGTTGAGCACCATTT+GGG 0.415520 4_2:+2668236 None:intergenic
TCATCTGCAAGCATTAGATT+TGG 0.416468 4_2:-2667291 Msa0583860:CDS
AAATTCGTGCAGTTCACAAA+TGG 0.417597 4_2:-2666300 Msa0583860:CDS
GAAGATTGCGGATTGAAGAT+TGG 0.417689 4_2:-2666195 Msa0583860:CDS
AATTCGTGCAGTTCACAAAT+GGG 0.433283 4_2:-2666299 Msa0583860:CDS
ATTAACCAGCAAAGAATCTC+TGG 0.438972 4_2:-2667929 Msa0583860:intron
GAAATCATGAACTCTACATA+AGG 0.443348 4_2:-2668523 Msa0583860:CDS
ATATCTTAACCTTAAAATAT+GGG 0.446492 4_2:+2668107 None:intergenic
GAACAATGACAAGGTTATGA+AGG 0.451012 4_2:-2666987 Msa0583860:intron
ACAAACCAGAGATTCTTTGC+TGG 0.459376 4_2:+2667924 None:intergenic
ATAAGGATATGTAATGCAAC+AGG 0.467639 4_2:+2668350 None:intergenic
GAGAGTTCCCAAGAAATTTG+TGG 0.469633 4_2:-2668302 Msa0583860:CDS
TTTGCAAAGTCTCTAAAAGA+TGG 0.473533 4_2:-2668160 Msa0583860:CDS
TTTGGCTTGGGTGATAGTTA+TGG 0.473644 4_2:+2667107 None:intergenic
ATGAGAATGAGAGAACTTGT+AGG 0.473892 4_2:-2666245 Msa0583860:CDS
TGTGTTTCAGCAGACTTAGA+AGG 0.481816 4_2:-2666507 Msa0583860:intron
TATTTGGTTTAAGAGAAATT+GGG 0.491470 4_2:-2668188 Msa0583860:CDS
GATATTTGGTCACAATACAT+TGG 0.502848 4_2:-2668089 Msa0583860:CDS
GATAATATTTGGAAGAAAAG+AGG 0.503729 4_2:-2666948 Msa0583860:CDS
AGAACTTGTAGGTTCTATGC+AGG 0.508431 4_2:-2666234 Msa0583860:CDS
AGAACATACTCGAGAGATCA+TGG 0.514858 4_2:+2666901 None:intergenic
AGAACATGCATTGTCACCTC+TGG 0.519227 4_2:-2667216 Msa0583860:CDS
CATAAATTTAAGGAAGATTG+CGG 0.529511 4_2:-2666207 Msa0583860:CDS
GAGGCATTGAAGGAAGTTCA+AGG 0.529967 4_2:-2666929 Msa0583860:CDS
AAATCATGAACTCTACATAA+GGG 0.530814 4_2:-2668522 Msa0583860:CDS
CTTGTAGGTTCTATGCAGGT+TGG 0.536785 4_2:-2666230 Msa0583860:CDS
TTCATAGATGAAAGTTGTGA+AGG 0.538273 4_2:-2668043 Msa0583860:CDS
ATAGTTATGGTGAATGAAAG+TGG 0.542900 4_2:+2667120 None:intergenic
ACACGAATCTCTATTGTGCT+CGG 0.549823 4_2:+2666468 None:intergenic
GTAATTCTTAGACTCCCAAA+TGG 0.561023 4_2:-2668250 Msa0583860:CDS
GATGGCAATGTTAATAGTGC+TGG 0.575875 4_2:-2667986 Msa0583860:CDS
CTTAGAAGGTGGTAATGTGA+TGG 0.576917 4_2:-2666493 Msa0583860:CDS
TGAGAACATTCCTATGAAAG+TGG 0.579488 4_2:-2667268 Msa0583860:CDS
GGCAAATGGCTTCCAACCAG+AGG 0.587307 4_2:+2667200 None:intergenic
CATGCATTGTCACCTCTGGT+TGG 0.594204 4_2:-2667212 Msa0583860:CDS
TATGCTCTTGAAAAGTCACT+TGG 0.596669 4_2:+2667336 None:intergenic
CATAAATAATCCTACCGGGT+AGG 0.597503 4_2:+2666437 None:intergenic
AAGAGACATAAAGAGTGTCA+TGG 0.604494 4_2:-2666324 Msa0583860:CDS
TTCGTGCAGTTCACAAATGG+GGG 0.622353 4_2:-2666297 Msa0583860:CDS
ATTCGTGCAGTTCACAAATG+GGG 0.622898 4_2:-2666298 Msa0583860:CDS
GTTTCAGCAGACTTAGAAGG+TGG 0.625758 4_2:-2666504 Msa0583860:intron
ATAGTGCTGGCACATCTCAA+AGG 0.625943 4_2:-2667973 Msa0583860:CDS
ATAACTATCACCCAAGCCAA+AGG 0.651162 4_2:-2667105 Msa0583860:intron
GCAGAACAAACAACTAGTGG+TGG 0.662011 4_2:+2666103 None:intergenic
TCGTGTGAACTCTACCTACC+CGG 0.663506 4_2:-2666451 Msa0583860:intron
GAAGTTGTTGAACTGAGTGA+TGG 0.670925 4_2:-2668004 Msa0583860:CDS
GAACATTCCTATGAAAGTGG+AGG 0.681609 4_2:-2667265 Msa0583860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAATAAAATAAAATTACA+AGG + chr4_2:2666817-2666836 None:intergenic 10.0%
!!! TGTAATTTTATTTTATTTTG+AGG - chr4_2:2666817-2666836 Msa0583860:intron 10.0%
!!! ATTTTTTAGAGATTTTAAAT+AGG - chr4_2:2667808-2667827 Msa0583860:intron 10.0%
!!! AAATAATTTGTAATATATGA+GGG + chr4_2:2667992-2668011 None:intergenic 10.0%
!!! TAAATAATTTGTAATATATG+AGG + chr4_2:2667993-2668012 None:intergenic 10.0%
!! ACAAATTATTTAATATTTCA+AGG - chr4_2:2668001-2668020 Msa0583860:CDS 10.0%
!! ATTATTAACAAATGTATAGT+TGG + chr4_2:2666279-2666298 None:intergenic 15.0%
!! ACAATAATCATTTATGAATA+AGG + chr4_2:2666349-2666368 None:intergenic 15.0%
!! ATATCTTAACCTTAAAATAT+GGG + chr4_2:2666575-2666594 None:intergenic 15.0%
!! AATATCTTAACCTTAAAATA+TGG + chr4_2:2666576-2666595 None:intergenic 15.0%
!!! TATTTTAAGGTTAAGATATT+TGG - chr4_2:2666576-2666595 Msa0583860:intron 15.0%
!!! TTCATCTATGAATTTTATAT+TGG + chr4_2:2666628-2666647 None:intergenic 15.0%
!!! TAAGTATAATGTTTTATAGT+AGG + chr4_2:2667024-2667043 None:intergenic 15.0%
!!! AAAAAAGTTACTAGTAATTT+TGG - chr4_2:2667275-2667294 Msa0583860:CDS 15.0%
!!! AACTTGTAAGTTATATTTTT+TGG + chr4_2:2667503-2667522 None:intergenic 15.0%
!! AAAAAATATAACTTACAAGT+TGG - chr4_2:2667502-2667521 Msa0583860:intron 15.0%
!!! CTTTTTTAAATAAAGTTTGA+AGG + chr4_2:2667605-2667624 None:intergenic 15.0%
!!! CTTTTCAAAAAATTATAACT+AGG - chr4_2:2667864-2667883 Msa0583860:intron 15.0%
!!! ATAAAATTTAAAACAGAACT+TGG + chr4_2:2667932-2667951 None:intergenic 15.0%
!!! ATATTTGGTTTAAGAGAAAT+TGG - chr4_2:2666490-2666509 Msa0583860:CDS 20.0%
!!! TATTTGGTTTAAGAGAAATT+GGG - chr4_2:2666491-2666510 Msa0583860:CDS 20.0%
!!! GCTTTAGTTTTCAAATATTT+AGG - chr4_2:2666546-2666565 Msa0583860:intron 20.0%
!!! TTAGTTTTCAAATATTTAGG+TGG - chr4_2:2666549-2666568 Msa0583860:intron 20.0%
!!! ATCTATTTTAGCCATTTATA+AGG + chr4_2:2666850-2666869 None:intergenic 20.0%
!!! TTTTTGTTTTTTAACTTTGC+AGG - chr4_2:2666869-2666888 Msa0583860:intron 20.0%
!!! AAGTTATATTTTTTGGCAAA+TGG + chr4_2:2667496-2667515 None:intergenic 20.0%
!! CAATTGATATTAAGCAAATT+TGG + chr4_2:2667654-2667673 None:intergenic 20.0%
!! TCTATTGTGATGATAATATT+TGG - chr4_2:2667720-2667739 Msa0583860:intron 20.0%
!!! CTTGAACTAGTTTTTTTAAT+TGG + chr4_2:2668029-2668048 None:intergenic 20.0%
!! TTTAACATAAATAATCCTAC+CGG + chr4_2:2668250-2668269 None:intergenic 20.0%
!! AGGATTATTTATGTTAAATG+TGG - chr4_2:2668252-2668271 Msa0583860:CDS 20.0%
! AAATCATGAACTCTACATAA+GGG - chr4_2:2666157-2666176 Msa0583860:CDS 25.0%
!! GATTGTGATTTGTAATTGAA+AGG + chr4_2:2666257-2666276 None:intergenic 25.0%
! TATTTCTCCACAAATTTCTT+GGG + chr4_2:2666387-2666406 None:intergenic 25.0%
! ATATTTCTCCACAAATTTCT+TGG + chr4_2:2666388-2666407 None:intergenic 25.0%
! AGAAATTTGTGGAGAAATAT+TGG - chr4_2:2666388-2666407 Msa0583860:intron 25.0%
!!! AAGTTTCTAAGGTTTTAGAT+AGG + chr4_2:2667114-2667133 None:intergenic 25.0%
! CTAAAACCTTAGAAACTTAA+AGG - chr4_2:2667116-2667135 Msa0583860:CDS 25.0%
! AAGTTTCAAAGGTTACATAT+AGG + chr4_2:2667143-2667162 None:intergenic 25.0%
!!! ATGTAACCTTTGAAACTTAA+AGG - chr4_2:2667145-2667164 Msa0583860:CDS 25.0%
! GTGAAATAGCCAATAAATAA+TGG + chr4_2:2667220-2667239 None:intergenic 25.0%
! TAAACCTTGAATTGAAATAG+AGG - chr4_2:2667317-2667336 Msa0583860:CDS 25.0%
! TATTTGAATGACAACCTTAA+AGG - chr4_2:2667364-2667383 Msa0583860:intron 25.0%
! ATTTGAATGACAACCTTAAA+GGG - chr4_2:2667365-2667384 Msa0583860:intron 25.0%
! GATAATATTTGGAAGAAAAG+AGG - chr4_2:2667731-2667750 Msa0583860:intron 25.0%
!! TAACTAGGTTTTCTTACTTT+AGG - chr4_2:2667879-2667898 Msa0583860:intron 25.0%
! TTAACATAAATAATCCTACC+GGG + chr4_2:2668249-2668268 None:intergenic 25.0%
!! TCATAATATTGCACTTTTAC+AGG + chr4_2:2668418-2668437 None:intergenic 25.0%
! CATAAATTTAAGGAAGATTG+CGG - chr4_2:2668472-2668491 Msa0583860:intron 25.0%
GAAATCATGAACTCTACATA+AGG - chr4_2:2666156-2666175 Msa0583860:CDS 30.0%
ATAAGGATATGTAATGCAAC+AGG + chr4_2:2666332-2666351 None:intergenic 30.0%
AGTCTAAGAATTACTGGATT+TGG + chr4_2:2666423-2666442 None:intergenic 30.0%
! TGCTCAACAGGAAATATTTT+GGG - chr4_2:2666452-2666471 Msa0583860:CDS 30.0%
! TTTGCAAAGTCTCTAAAAGA+TGG - chr4_2:2666519-2666538 Msa0583860:intron 30.0%
GATATTTGGTCACAATACAT+TGG - chr4_2:2666590-2666609 Msa0583860:intron 30.0%
TTCATAGATGAAAGTTGTGA+AGG - chr4_2:2666636-2666655 Msa0583860:intron 30.0%
! GAGGTATTCTACCTTATAAA+TGG - chr4_2:2666836-2666855 Msa0583860:intron 30.0%
!!! TGTTTTTTAACTTTGCAGGT+AGG - chr4_2:2666873-2666892 Msa0583860:intron 30.0%
! GACAATTCAAGTTCAAAACT+TGG + chr4_2:2666968-2666987 None:intergenic 30.0%
! ATAGGTCCTTTAAGTTTCTA+AGG + chr4_2:2667125-2667144 None:intergenic 30.0%
! ATAGGTCCTTTAAGTTTCAA+AGG + chr4_2:2667154-2667173 None:intergenic 30.0%
GTTACAAACGTGAAACTTAA+AGG - chr4_2:2667176-2667195 Msa0583860:CDS 30.0%
GGTATTTGGCCATTATTTAT+TGG - chr4_2:2667208-2667227 Msa0583860:CDS 30.0%
GTATCCTCTATTTCAATTCA+AGG + chr4_2:2667324-2667343 None:intergenic 30.0%
ATAGTTATGGTGAATGAAAG+TGG + chr4_2:2667562-2667581 None:intergenic 30.0%
AAGTTTGAAGGAAAAACCTT+TGG + chr4_2:2667593-2667612 None:intergenic 30.0%
TTGTCATTGTTCAAAGGAAT+TGG + chr4_2:2667684-2667703 None:intergenic 30.0%
AATTCCTTTGAACAATGACA+AGG - chr4_2:2667683-2667702 Msa0583860:intron 30.0%
ATAACCTTGTCATTGTTCAA+AGG + chr4_2:2667690-2667709 None:intergenic 30.0%
ATTTGTAATATATGAGGGAG+AGG + chr4_2:2667987-2668006 None:intergenic 30.0%
ACTATCTTCAAGAACTCTTC+TGG + chr4_2:2666134-2666153 None:intergenic 35.0%
TTTGGGAGTCTAAGAATTAC+TGG + chr4_2:2666429-2666448 None:intergenic 35.0%
GTAATTCTTAGACTCCCAAA+TGG - chr4_2:2666429-2666448 Msa0583860:intron 35.0%
! GTGCTCAACAGGAAATATTT+TGG - chr4_2:2666451-2666470 Msa0583860:intron 35.0%
!! TCAACAGGAAATATTTTGGG+TGG - chr4_2:2666455-2666474 Msa0583860:CDS 35.0%
! TTTAGGTGGCCCATATTTTA+AGG - chr4_2:2666563-2666582 Msa0583860:intron 35.0%
ACATCTCAAAGGAGAAAAAG+TGG - chr4_2:2666717-2666736 Msa0583860:intron 35.0%
ATTAACCAGCAAAGAATCTC+TGG - chr4_2:2666750-2666769 Msa0583860:intron 35.0%
! ACAAAGGAGGCATAATTAAG+AGG - chr4_2:2666897-2666916 Msa0583860:intron 35.0%
! CAAAGGAGGCATAATTAAGA+GGG - chr4_2:2666898-2666917 Msa0583860:intron 35.0%
! TTACGCTCATGGCTATATTT+TGG - chr4_2:2666994-2667013 Msa0583860:intron 35.0%
!! GTTTCGAGTTTGTTTCAGAT+AGG - chr4_2:2667062-2667081 Msa0583860:intron 35.0%
!!! GGTTTTAGATAGGTCCTTAA+CGG + chr4_2:2667104-2667123 None:intergenic 35.0%
GTTTCACGTTTGTAACAGAT+AGG + chr4_2:2667172-2667191 None:intergenic 35.0%
TATGCTCTTGAAAAGTCACT+TGG + chr4_2:2667346-2667365 None:intergenic 35.0%
TCATCTGCAAGCATTAGATT+TGG - chr4_2:2667388-2667407 Msa0583860:intron 35.0%
TGAGAACATTCCTATGAAAG+TGG - chr4_2:2667411-2667430 Msa0583860:intron 35.0%
GAACAATGACAAGGTTATGA+AGG - chr4_2:2667692-2667711 Msa0583860:intron 35.0%
!! AAGAGACATAAAGAGTGTCA+TGG - chr4_2:2668355-2668374 Msa0583860:CDS 35.0%
AAATTCGTGCAGTTCACAAA+TGG - chr4_2:2668379-2668398 Msa0583860:intron 35.0%
AATTCGTGCAGTTCACAAAT+GGG - chr4_2:2668380-2668399 Msa0583860:intron 35.0%
ATGAGAATGAGAGAACTTGT+AGG - chr4_2:2668434-2668453 Msa0583860:intron 35.0%
! TGCAGGTTGGCATAAATTTA+AGG - chr4_2:2668462-2668481 Msa0583860:intron 35.0%
GAGAGTTCCCAAGAAATTTG+TGG - chr4_2:2666377-2666396 Msa0583860:intron 40.0%
TTTCCTGTTGAGCACCATTT+GGG + chr4_2:2666446-2666465 None:intergenic 40.0%
ATTTCCTGTTGAGCACCATT+TGG + chr4_2:2666447-2666466 None:intergenic 40.0%
GAAGTTGTTGAACTGAGTGA+TGG - chr4_2:2666675-2666694 Msa0583860:intron 40.0%
GATGGCAATGTTAATAGTGC+TGG - chr4_2:2666693-2666712 Msa0583860:intron 40.0%
ACAAACCAGAGATTCTTTGC+TGG + chr4_2:2666758-2666777 None:intergenic 40.0%
AACTTTGCAGGTAGGAACAA+AGG - chr4_2:2666881-2666900 Msa0583860:intron 40.0%
TTGTCACAATCTTACGCTCA+TGG - chr4_2:2666983-2667002 Msa0583860:intron 40.0%
!! CTTAACGGAGGTTGATAGAA+AGG + chr4_2:2667089-2667108 None:intergenic 40.0%
CTTTCTATCAACCTCCGTTA+AGG - chr4_2:2667087-2667106 Msa0583860:intron 40.0%
TTTAGATAGGTCCTTAACGG+AGG + chr4_2:2667101-2667120 None:intergenic 40.0%
TAATGGCCAAATACCTTCCT+GGG + chr4_2:2667203-2667222 None:intergenic 40.0%
ATAATGGCCAAATACCTTCC+TGG + chr4_2:2667204-2667223 None:intergenic 40.0%
GAACATTCCTATGAAAGTGG+AGG - chr4_2:2667414-2667433 Msa0583860:intron 40.0%
AACTTCACCTCCACTTTCAT+AGG + chr4_2:2667424-2667443 None:intergenic 40.0%
!! TTTGGCTTGGGTGATAGTTA+TGG + chr4_2:2667575-2667594 None:intergenic 40.0%
ATAACTATCACCCAAGCCAA+AGG - chr4_2:2667574-2667593 Msa0583860:intron 40.0%
! GAAGGAAAAACCTTTGGCTT+GGG + chr4_2:2667587-2667606 None:intergenic 40.0%
TGAAGGAAAAACCTTTGGCT+TGG + chr4_2:2667588-2667607 None:intergenic 40.0%
!! TGGAAGAAAAGAGGCATTGA+AGG - chr4_2:2667740-2667759 Msa0583860:intron 40.0%
AGAACATACTCGAGAGATCA+TGG + chr4_2:2667781-2667800 None:intergenic 40.0%
TGTGTTTCAGCAGACTTAGA+AGG - chr4_2:2668172-2668191 Msa0583860:CDS 40.0%
! CTTAGAAGGTGGTAATGTGA+TGG - chr4_2:2668186-2668205 Msa0583860:CDS 40.0%
ACACGAATCTCTATTGTGCT+CGG + chr4_2:2668214-2668233 None:intergenic 40.0%
CATAAATAATCCTACCGGGT+AGG + chr4_2:2668245-2668264 None:intergenic 40.0%
ATTCGTGCAGTTCACAAATG+GGG - chr4_2:2668381-2668400 Msa0583860:intron 40.0%
TCACAAATGGGGGAAAATCA+TGG - chr4_2:2668392-2668411 Msa0583860:intron 40.0%
AGAACTTGTAGGTTCTATGC+AGG - chr4_2:2668445-2668464 Msa0583860:intron 40.0%
! GAAGATTGCGGATTGAAGAT+TGG - chr4_2:2668484-2668503 Msa0583860:intron 40.0%
!! ATATTTTGGGTGGAGCGTGA+CGG - chr4_2:2666465-2666484 Msa0583860:CDS 45.0%
!! ATAGTGCTGGCACATCTCAA+AGG - chr4_2:2666706-2666725 Msa0583860:intron 45.0%
TTTGCAGGTAGGAACAAAGG+AGG - chr4_2:2666884-2666903 Msa0583860:intron 45.0%
ACGTGAAACTTAAAGGACCC+AGG - chr4_2:2667183-2667202 Msa0583860:CDS 45.0%
GAAACTTAAAGGACCCAGGA+AGG - chr4_2:2667187-2667206 Msa0583860:CDS 45.0%
!! AAAGGACCCAGGAAGGTATT+TGG - chr4_2:2667194-2667213 Msa0583860:CDS 45.0%
TGCTTGCAGATGACCCTTTA+AGG + chr4_2:2667381-2667400 None:intergenic 45.0%
AGAACATGCATTGTCACCTC+TGG - chr4_2:2667463-2667482 Msa0583860:intron 45.0%
!! GAGGCATTGAAGGAAGTTCA+AGG - chr4_2:2667750-2667769 Msa0583860:intron 45.0%
GTTTCAGCAGACTTAGAAGG+TGG - chr4_2:2668175-2668194 Msa0583860:CDS 45.0%
TTCGTGCAGTTCACAAATGG+GGG - chr4_2:2668382-2668401 Msa0583860:intron 45.0%
CTTGTAGGTTCTATGCAGGT+TGG - chr4_2:2668449-2668468 Msa0583860:intron 45.0%
ACTCCCAAATGGTGCTCAAC+AGG - chr4_2:2666440-2666459 Msa0583860:intron 50.0%
TGGAGCGTGACGGTGATATT+TGG - chr4_2:2666475-2666494 Msa0583860:CDS 50.0%
CATGCATTGTCACCTCTGGT+TGG - chr4_2:2667467-2667486 Msa0583860:intron 50.0%
TCGTGTGAACTCTACCTACC+CGG - chr4_2:2668228-2668247 Msa0583860:CDS 50.0%
GGCAAATGGCTTCCAACCAG+AGG + chr4_2:2667482-2667501 None:intergenic 55.0%
GTGAACTCTACCTACCCGGT+AGG - chr4_2:2668232-2668251 Msa0583860:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 2666116 2668585 2666116 ID=Msa0583860;Name=Msa0583860
chr4_2 mRNA 2666116 2668585 2666116 ID=Msa0583860-mRNA-1;Parent=Msa0583860;Name=Msa0583860-mRNA-1;_AED=0.11;_eAED=0.13;_QI=0|0|0|0.85|0.5|0.71|7|0|403
chr4_2 exon 2668515 2668585 2668515 ID=Msa0583860-mRNA-1:exon:768;Parent=Msa0583860-mRNA-1
chr4_2 exon 2667930 2668381 2667930 ID=Msa0583860-mRNA-1:exon:767;Parent=Msa0583860-mRNA-1
chr4_2 exon 2667811 2667833 2667811 ID=Msa0583860-mRNA-1:exon:766;Parent=Msa0583860-mRNA-1
chr4_2 exon 2667106 2667364 2667106 ID=Msa0583860-mRNA-1:exon:765;Parent=Msa0583860-mRNA-1
chr4_2 exon 2666910 2666996 2666910 ID=Msa0583860-mRNA-1:exon:764;Parent=Msa0583860-mRNA-1
chr4_2 exon 2666452 2666519 2666452 ID=Msa0583860-mRNA-1:exon:763;Parent=Msa0583860-mRNA-1
chr4_2 exon 2666116 2666367 2666116 ID=Msa0583860-mRNA-1:exon:762;Parent=Msa0583860-mRNA-1
chr4_2 CDS 2668515 2668585 2668515 ID=Msa0583860-mRNA-1:cds;Parent=Msa0583860-mRNA-1
chr4_2 CDS 2667930 2668381 2667930 ID=Msa0583860-mRNA-1:cds;Parent=Msa0583860-mRNA-1
chr4_2 CDS 2667811 2667833 2667811 ID=Msa0583860-mRNA-1:cds;Parent=Msa0583860-mRNA-1
chr4_2 CDS 2667106 2667364 2667106 ID=Msa0583860-mRNA-1:cds;Parent=Msa0583860-mRNA-1
chr4_2 CDS 2666910 2666996 2666910 ID=Msa0583860-mRNA-1:cds;Parent=Msa0583860-mRNA-1
chr4_2 CDS 2666452 2666519 2666452 ID=Msa0583860-mRNA-1:cds;Parent=Msa0583860-mRNA-1
chr4_2 CDS 2666116 2666367 2666116 ID=Msa0583860-mRNA-1:cds;Parent=Msa0583860-mRNA-1
Gene Sequence

>Msa0583860

ATGTCATTACATCATCCAGAAGAGTTCTTGAAGATAGTTCGAAATCATGAACTCTACATAAGGGAGAGCTTTTTCTTCTTCCTGTTGCATTACATATCCTTATTCATAAATGATTATTGTCTGTTGCAGAGAGTTCCCAAGAAATTTGTGGAGAAATATTGGAAAAGAATACCAAATCCAGTAATTCTTAGACTCCCAAATGGTGCTCAACAGGAAATATTTTGGGTGGAGCGTGACGGTGATATTTGGTTTAAGAGAAATTGGGAAAAATTTGCAAAGTCTCTAAAAGATGGATATGCTTTAGTTTTCAAATATTTAGGTGGCCCATATTTTAAGGTTAAGATATTTGGTCACAATACATTGGAAAAAGACTATTCCAATATAAAATTCATAGATGAAAGTTGTGAAGGTAGAGAAGAAGTTCAAGAAGTTGTTGAACTGAGTGATGGCAATGTTAATAGTGCTGGCACATCTCAAAGGAGAAAAAGTGGCAAGAGAAAAATTAACCAGCAAAGAATCTCTGATTTTTGTTTTTTAACTTTGCAGAGGATACCAAGTGACTTTTCAAGAGCATATTTGAATGACAACCTTAAAGGGTCATCTGCAAGCATTAGATTTGGTGAGAACATTCCTATGAAAGTGGAGGTGAAGTTTCAAGATATCAAGAATAATAGAACATGCATTGTCACCTCTGGTTGGAAGCCATTTGCCAAAAAATATAACTTACAAGTTGGCAATGTATGTAAATTTGTGATGACTCAACTTCAACCACTTTCATTCACCATAACTATCACCCAAGCCAAAGGTTATGAAGGAATAATCTATTGTGATGATAATATTTGGAAGAAAAGAGGCATTGAAGGAAGTTCAAGGAGCTGCCATGATCTCTCGACAGACTTAGAAGGTGGTAATGTGATGGAACCGAGCACAATAGAGATTCGTGTGAACTCTACCTACCCGTACATTAAACATGAGTTTCTTAAGAGACATAAAGAGTGTCATGGAAAATTCGTGCAGTTCACAAATGGGGGAAAATCATGGCCTGTAAAAGTGCAATATTATGAGAATGAGAGAACTTGTAGGTTCTATGCAGGTTGGCATAAATTTAAGGAAGATTGCGGATTGAAGATTGGAGATACTTGCAAATTGAAGTTGATTGATGAAAAAATGTTTGTGTTTAAAGTTTCAATTGAAAGAATGCACCACCACTAG
Protein sequence

>Msa0583860

MSLHHPEEFLKIVRNHELYIRESFFFFLLHYISLFINDYCLLQRVPKKFVEKYWKRIPNPVILRLPNGAQQEIFWVERDGDIWFKRNWEKFAKSLKDGYALVFKYLGGPYFKVKIFGHNTLEKDYSNIKFIDESCEGREEVQEVVELSDGNVNSAGTSQRRKSGKRKINQQRISDFCFLTLQRIPSDFSRAYLNDNLKGSSASIRFGENIPMKVEVKFQDIKNNRTCIVTSGWKPFAKKYNLQVGNVCKFVMTQLQPLSFTITITQAKGYEGIIYCDDNIWKKRGIEGSSRSCHDLSTDLEGGNVMEPSTIEIRVNSTYPYIKHEFLKRHKECHGKFVQFTNGGKSWPVKVQYYENERTCRFYAGWHKFKEDCGLKIGDTCKLKLIDEKMFVFKVSIERMHHH*