AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0584020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0584020 MtrunA17_Chr2g0330591 60.982 387 62 5 1 299 261 646 3.86e-149 440
Msa0584020 MtrunA17_Chr2g0330621 77.841 176 26 2 1 163 28 203 5.79e-92 271
Msa0584020 MtrunA17_Chr2g0330631 76.582 158 17 1 162 299 2 159 1.87e-79 241
Msa0584020 MtrunA17_Chr8g0341151 35.844 385 154 11 1 299 255 632 1.43e-58 201
Msa0584020 MtrunA17_Chr8g0340931 36.010 386 155 11 1 299 259 639 1.65e-58 201
Msa0584020 MtrunA17_Chr8g0341011 35.401 387 158 11 1 299 260 642 3.94e-57 197
Msa0584020 MtrunA17_Chr8g0340871 34.704 389 159 11 1 299 265 648 1.55e-56 195
Msa0584020 MtrunA17_Chr8g0340891 34.031 382 154 9 5 299 267 637 2.91e-56 194
Msa0584020 MtrunA17_Chr8g0347661 34.465 383 162 11 1 299 267 644 1.62e-55 192
Msa0584020 MtrunA17_Chr8g0340911 34.656 378 161 8 5 299 265 639 4.01e-55 191
Msa0584020 MtrunA17_Chr8g0340881 34.439 392 153 11 1 299 263 643 1.26e-51 181
Msa0584020 MtrunA17_Chr6g0487571 31.579 380 173 8 4 299 267 643 7.47e-48 171
Msa0584020 MtrunA17_Chr8g0358831 34.454 357 147 9 27 299 254 607 1.89e-46 167
Msa0584020 MtrunA17_Chr8g0341101 32.692 364 159 11 16 299 332 689 7.28e-44 159
Msa0584020 MtrunA17_Chr8g0341061 33.155 374 165 13 6 299 318 686 8.61e-41 150
Msa0584020 MtrunA17_Chr8g0341361 35.249 261 80 6 127 299 1 260 6.66e-36 134
Msa0584020 MtrunA17_Chr8g0341111 40.000 165 71 2 160 299 391 552 2.34e-28 114
Msa0584020 MtrunA17_Chr8g0340941 29.897 291 119 9 1 220 259 535 4.41e-28 113
Msa0584020 MtrunA17_Chr8g0341231 40.341 176 82 6 18 172 298 471 7.44e-27 110
Msa0584020 MtrunA17_Chr8g0340971 39.333 150 68 4 169 299 17 162 7.44e-26 103
Msa0584020 MtrunA17_Chr8g0341131 54.878 82 37 0 218 299 9 90 1.18e-23 94.7
Msa0584020 MtrunA17_Chr8g0341081 52.874 87 41 0 213 299 34 120 3.57e-23 97.4
Msa0584020 MtrunA17_Chr8g0341221 55.172 87 38 1 214 299 24 110 1.45e-22 93.2
Msa0584020 MtrunA17_Chr8g0341251 51.724 87 42 0 213 299 35 121 6.81e-22 94.0
Msa0584020 MtrunA17_Chr8g0340951 50.575 87 43 0 213 299 29 115 6.84e-22 91.3
Msa0584020 MtrunA17_Chr2g0330691 86.364 44 6 0 256 299 1 44 1.12e-18 82.8
Msa0584020 MtrunA17_Chr2g0330701 73.333 45 12 0 175 219 29 73 4.05e-17 74.7
Msa0584020 MtrunA17_Chr8g0341141 31.551 187 109 6 1 169 261 446 5.09e-17 81.3
Msa0584020 MtrunA17_Chr8g0341171 30.675 163 70 5 156 299 2 140 1.95e-16 75.1
Msa0584020 MtrunA17_Chr7g0263591 23.367 398 183 11 19 299 284 676 1.25e-15 77.4
Msa0584020 MtrunA17_Chr5g0423891 33.333 159 85 2 161 299 78 235 1.58e-15 76.6
Msa0584020 MtrunA17_Chr8g0341091 32.530 166 96 4 5 159 315 475 5.14e-15 75.5
Msa0584020 MtrunA17_Chr8g0376131 45.238 84 44 1 218 299 41 124 5.31e-13 68.9
Msa0584020 MtrunA17_Chr8g0365691 42.667 75 41 1 218 290 300 374 1.27e-12 67.8
Msa0584020 MtrunA17_Chr1g0181731 41.176 85 50 0 215 299 586 670 3.05e-12 67.0
Msa0584020 MtrunA17_Chr6g0455651 40.244 82 49 0 218 299 107 188 3.84e-12 66.2
Msa0584020 MtrunA17_Chr8g0373501 38.095 84 50 1 218 299 326 409 4.87e-12 66.2
Msa0584020 MtrunA17_Chr7g0237401 40.000 85 48 2 218 299 82 166 7.92e-12 65.5
Msa0584020 MtrunA17_Chr8g0352381 36.905 84 51 1 218 299 520 603 8.54e-12 65.9
Msa0584020 MtrunA17_Chr6g0485721 39.759 83 48 1 218 298 87 169 8.57e-12 65.1
Msa0584020 MtrunA17_Chr6g0485761 39.286 84 49 1 218 299 284 367 9.73e-12 65.5
Msa0584020 MtrunA17_Chr2g0318301 35.714 84 52 1 218 299 491 574 1.61e-11 65.1
Msa0584020 MtrunA17_Chr1g0160771 30.097 206 107 7 123 299 175 372 1.79e-11 64.7
Msa0584020 MtrunA17_Chr7g0219671 39.506 81 47 1 221 299 525 605 2.23e-11 64.3
Msa0584020 MtrunA17_Chr5g0432871 33.621 116 61 3 197 296 571 686 2.51e-11 64.3
Msa0584020 MtrunA17_Chr6g0487561 35.780 109 65 3 2 107 274 380 2.83e-11 63.5
Msa0584020 MtrunA17_Chr7g0219601 30.337 178 92 5 150 299 418 591 3.32e-11 63.9
Msa0584020 MtrunA17_Chr7g0219781 39.506 81 47 1 221 299 528 608 3.58e-11 63.9
Msa0584020 MtrunA17_Chr1g0190581 36.905 84 51 1 218 299 511 594 3.79e-11 63.9
Msa0584020 MtrunA17_Chr7g0219571 29.677 155 82 4 147 299 478 607 4.53e-11 63.5
Msa0584020 MtrunA17_Chr3g0123141 41.791 67 39 0 226 292 607 673 4.72e-11 63.5
Msa0584020 MtrunA17_Chr5g0441021 37.234 94 47 2 218 299 59 152 4.88e-11 62.8
Msa0584020 MtrunA17_Chr7g0219631 37.975 79 47 1 218 294 51 129 5.72e-11 59.3
Msa0584020 MtrunA17_Chr3g0144481 42.667 75 41 1 218 290 78 152 6.21e-11 62.8
Msa0584020 MtrunA17_Chr7g0219721 40.741 81 46 1 221 299 525 605 7.41e-11 62.8
Msa0584020 MtrunA17_Chr1g0203631 38.272 81 48 1 221 299 985 1065 7.58e-11 63.2
Msa0584020 MtrunA17_Chr8g0341181 52.830 53 24 1 118 169 21 73 8.34e-11 58.9
Msa0584020 MtrunA17_Chr7g0219691 40.741 81 46 1 221 299 525 605 8.34e-11 62.8
Msa0584020 MtrunA17_Chr7g0219651 28.676 136 71 2 190 299 1457 1592 8.84e-11 62.8
Msa0584020 MtrunA17_Chr8g0341271 35.135 111 67 2 1 110 191 297 8.84e-11 61.6
Msa0584020 MtrunA17_Chr5g0403891 38.095 84 50 1 218 299 245 328 8.93e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0584020 AT2G19210.1 38.083 386 149 10 2 299 264 647 6.77e-66 221
Msa0584020 AT1G51870.1 39.157 332 147 7 19 299 275 602 2.67e-60 206
Msa0584020 AT1G51880.1 38.875 391 138 12 1 299 256 637 3.61e-60 206
Msa0584020 AT1G51880.2 38.303 389 143 11 1 299 274 655 4.51e-60 206
Msa0584020 AT1G51880.3 38.095 399 138 12 1 299 274 663 5.68e-59 202
Msa0584020 AT2G19230.2 34.865 370 158 10 11 299 273 640 1.28e-57 199
Msa0584020 AT2G19230.1 34.771 371 158 10 11 299 273 641 3.44e-57 198
Msa0584020 AT4G29990.2 32.732 388 169 9 2 299 260 645 6.07e-56 192
Msa0584020 AT4G29990.1 32.732 388 169 9 2 299 260 645 1.22e-55 193
Msa0584020 AT1G51860.2 36.693 387 143 12 14 299 126 511 5.42e-55 190
Msa0584020 AT1G51890.4 35.013 377 152 9 15 299 202 577 2.07e-54 189
Msa0584020 AT1G51890.3 35.013 377 152 9 15 299 266 641 2.07e-54 188
Msa0584020 AT1G51870.2 35.696 381 144 8 19 299 275 654 2.54e-54 189
Msa0584020 AT1G51860.1 36.693 387 143 12 14 299 270 655 3.08e-54 189
Msa0584020 AT1G51890.1 35.013 377 152 9 15 299 266 641 3.57e-54 189
Msa0584020 AT1G51890.2 35.013 377 152 9 15 299 266 641 4.62e-54 189
Msa0584020 AT1G51805.2 32.598 408 156 11 2 299 227 625 1.11e-53 187
Msa0584020 AT1G51805.1 32.598 408 156 11 2 299 251 649 1.43e-53 187
Msa0584020 AT1G05700.1 33.967 368 155 8 18 299 276 641 4.33e-53 186
Msa0584020 AT1G51890.5 34.865 370 148 9 22 299 1 369 5.66e-53 182
Msa0584020 AT1G51810.1 32.250 400 169 9 1 299 124 522 1.81e-50 177
Msa0584020 AT1G51850.1 31.683 404 169 10 1 299 229 630 2.21e-49 176
Msa0584020 AT3G21340.2 31.671 401 163 11 7 299 267 664 2.47e-49 174
Msa0584020 AT1G51850.2 31.683 404 169 10 1 299 249 650 2.49e-49 176
Msa0584020 AT3G21340.1 31.671 401 163 11 7 299 267 664 5.58e-49 174
Msa0584020 AT1G05700.2 31.328 399 155 8 18 299 276 672 1.05e-48 174
Msa0584020 AT1G51820.1 30.693 404 173 9 1 299 249 650 1.31e-48 173
Msa0584020 AT1G51830.1 31.750 400 169 11 1 299 62 458 4.94e-48 171
Msa0584020 AT1G51830.2 31.750 400 169 11 1 299 69 465 5.42e-48 170
Msa0584020 AT2G19190.1 32.808 381 168 9 5 299 267 645 6.45e-47 169
Msa0584020 AT1G51910.1 35.904 376 141 11 19 299 275 645 9.53e-47 168
Msa0584020 AT3G46400.1 32.143 392 172 11 1 299 258 648 4.78e-46 166
Msa0584020 AT3G46420.1 35.673 342 158 12 13 299 269 603 9.10e-46 165
Msa0584020 AT1G07560.1 32.703 370 156 11 19 299 277 642 2.45e-45 164
Msa0584020 AT3G46330.2 32.647 340 169 9 19 299 276 614 2.46e-44 161
Msa0584020 AT5G59680.1 32.637 383 153 9 18 299 274 652 3.50e-44 160
Msa0584020 AT2G28970.2 31.407 398 173 9 1 299 120 516 6.38e-44 159
Msa0584020 AT2G28970.1 31.407 398 173 9 1 299 155 551 8.01e-44 159
Msa0584020 AT4G20450.2 31.806 371 163 9 19 299 293 663 8.06e-44 160
Msa0584020 AT1G51820.2 30.607 379 156 9 26 299 12 388 8.63e-44 158
Msa0584020 AT4G20450.1 31.806 371 163 9 19 299 293 663 1.12e-43 159
Msa0584020 AT1G49100.1 28.788 396 175 8 6 299 263 653 6.43e-42 154
Msa0584020 AT1G51800.1 30.691 391 157 10 18 299 272 657 9.80e-42 154
Msa0584020 AT3G46330.1 31.148 366 164 10 19 299 276 638 2.91e-41 152
Msa0584020 AT2G14440.1 29.843 382 177 11 2 299 261 635 7.75e-41 151
Msa0584020 AT2G28990.2 30.100 402 174 9 1 299 252 649 2.94e-40 149
Msa0584020 AT2G28990.1 30.100 402 174 9 1 299 252 649 2.94e-40 149
Msa0584020 AT5G59650.1 32.663 398 166 12 1 299 257 651 3.85e-40 149
Msa0584020 AT2G29000.1 31.892 370 160 9 19 299 271 637 2.97e-39 147
Msa0584020 AT1G51790.3 26.943 386 190 9 5 299 264 648 3.85e-39 146
Msa0584020 AT1G51790.1 26.943 386 190 9 5 299 264 648 3.85e-39 146
Msa0584020 AT2G14510.1 29.659 381 181 12 1 299 259 634 6.57e-39 145
Msa0584020 AT2G14510.2 29.659 381 181 12 1 299 267 642 7.01e-39 145
Msa0584020 AT5G59670.2 30.890 382 181 12 1 299 255 636 3.32e-38 144
Msa0584020 AT5G59670.1 30.890 382 181 12 1 299 255 636 3.32e-38 144
Msa0584020 AT5G16900.4 28.117 377 175 8 19 299 83 459 6.62e-38 142
Msa0584020 AT1G07550.1 30.871 379 178 13 1 299 257 631 1.22e-37 142
Msa0584020 AT5G16900.1 28.117 377 175 8 19 299 269 645 1.66e-37 141
Msa0584020 AT5G16900.3 28.117 377 175 8 19 299 269 645 1.66e-37 141
Msa0584020 AT5G59650.2 31.034 406 170 13 1 299 257 659 7.18e-36 137
Msa0584020 AT4G29180.3 30.570 386 173 12 5 299 268 649 8.45e-34 131
Msa0584020 AT4G29180.2 30.334 389 173 12 5 299 268 652 1.47e-33 130
Msa0584020 AT5G59660.2 32.911 316 122 9 49 275 222 536 3.13e-31 123
Msa0584020 AT5G59660.1 32.911 316 122 9 49 275 244 558 3.26e-31 123
Msa0584020 AT4G29180.1 29.016 386 180 11 5 299 268 650 5.17e-31 123
Msa0584020 AT5G59660.3 32.602 319 122 9 49 275 244 561 8.68e-31 122
Msa0584020 AT5G16900.2 26.923 364 170 8 19 286 269 632 1.27e-30 121
Msa0584020 AT3G46370.2 38.947 190 90 8 2 172 176 358 1.74e-25 106
Msa0584020 AT3G46370.2 45.000 100 55 0 200 299 460 559 3.45e-20 91.3
Msa0584020 AT3G46370.1 38.947 190 90 8 2 172 176 358 1.74e-25 106
Msa0584020 AT3G46370.1 45.000 100 55 0 200 299 460 559 3.45e-20 91.3
Msa0584020 AT2G04300.2 53.333 90 42 0 210 299 565 654 9.76e-25 104
Msa0584020 AT2G04300.2 35.979 189 97 9 4 172 267 451 6.97e-23 99.4
Msa0584020 AT2G04300.1 53.333 90 42 0 210 299 524 613 1.03e-24 104
Msa0584020 AT2G04300.1 30.811 185 71 7 4 172 267 410 7.71e-15 75.5
Msa0584020 AT3G46340.1 36.598 194 99 7 1 172 258 449 2.39e-24 103
Msa0584020 AT3G46340.1 48.352 91 46 1 210 299 568 658 1.66e-19 89.4
Msa0584020 AT5G59616.1 39.726 146 61 5 18 143 12 150 4.49e-23 93.2
Msa0584020 AT1G51790.2 30.978 184 111 4 5 172 264 447 1.11e-20 92.8
Msa0584020 AT1G51790.2 39.175 97 58 1 203 299 605 700 8.40e-14 72.4
Msa0584020 AT2G28960.1 45.556 90 49 0 210 299 424 513 1.91e-19 89.0
Msa0584020 AT2G28960.1 37.423 163 82 7 20 163 142 303 4.55e-17 82.0
Msa0584020 AT2G28960.2 45.556 90 49 0 210 299 556 645 2.37e-19 89.0
Msa0584020 AT2G28960.2 37.423 163 82 7 20 163 274 435 5.58e-17 81.6
Msa0584020 AT4G29450.1 33.146 178 85 5 148 299 481 650 2.12e-15 77.0
Msa0584020 AT4G29450.1 29.134 127 80 2 18 134 281 407 2.24e-12 67.8
Msa0584020 AT1G51620.2 45.556 90 48 1 210 299 30 118 1.14e-14 73.9
Msa0584020 AT5G28680.1 42.857 84 46 1 218 299 510 593 7.79e-13 69.3
Msa0584020 AT5G28680.2 42.857 84 46 1 218 299 510 593 7.79e-13 69.3
Msa0584020 AT3G04690.1 42.857 84 46 1 218 299 506 589 1.03e-12 68.9
Msa0584020 AT4G39110.1 39.286 84 49 1 218 299 515 598 2.40e-12 67.8
Msa0584020 AT4G32710.1 42.857 84 46 1 218 299 378 461 7.17e-12 66.2
Msa0584020 AT1G49270.1 36.905 84 51 1 218 299 325 408 9.45e-12 65.9
Msa0584020 AT1G10620.1 44.000 75 40 1 218 290 359 433 9.81e-12 65.9
Msa0584020 AT2G21480.1 36.905 84 51 1 218 299 514 597 1.06e-11 65.9
Msa0584020 AT5G61350.1 39.759 83 48 1 219 299 515 597 1.72e-11 65.1
Msa0584020 AT1G79620.3 45.333 75 39 1 218 290 627 701 1.72e-11 65.1
Msa0584020 AT1G23540.1 41.333 75 42 1 218 290 360 434 1.86e-11 65.1
Msa0584020 AT1G79620.2 45.333 75 39 1 218 290 627 701 1.91e-11 65.1
Msa0584020 AT1G23540.2 41.333 75 42 1 218 290 360 434 2.05e-11 64.7
Msa0584020 AT2G37050.2 29.891 184 87 6 148 296 497 673 2.13e-11 64.7
Msa0584020 AT1G79620.1 45.333 75 39 1 218 290 627 701 2.25e-11 65.1
Msa0584020 AT1G79620.5 45.333 75 39 1 218 290 653 727 2.34e-11 64.7
Msa0584020 AT3G24550.1 39.286 84 49 1 218 299 269 352 2.49e-11 64.7
Msa0584020 AT2G37050.1 29.891 184 87 6 148 296 497 673 3.26e-11 64.3
Msa0584020 AT1G70460.1 41.333 75 42 1 218 290 342 416 4.91e-11 63.9
Msa0584020 AT2G37050.4 29.189 185 88 5 148 296 497 674 5.25e-11 63.5
Msa0584020 AT5G54590.1 39.024 82 50 0 218 299 104 185 7.46e-11 62.0
Msa0584020 AT2G37050.3 29.189 185 88 5 148 296 497 674 7.68e-11 63.2
Msa0584020 AT3G24540.1 31.783 129 65 4 192 299 129 255 9.85e-11 62.8

Find 63 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAACTTCCAGCGGTTTAAT+TGG 0.192363 4_2:+2936564 Msa0584020:intron
TTTGATTTGCATCGAGCTTC+TGG 0.227717 4_2:-2934878 None:intergenic
AAATACTTGACAGGTCTTAT+AGG 0.242464 4_2:+2938287 Msa0584020:CDS
CTAAATGTGAAGTCAACTTA+TGG 0.264476 4_2:+2936367 Msa0584020:intron
TTAATTGGAACTATAGCTTC+TGG 0.326254 4_2:+2936579 Msa0584020:CDS
AATACTTGACAGGTCTTATA+GGG 0.376188 4_2:+2938288 Msa0584020:CDS
CAATGTCGTCGAGACATATA+TGG 0.381553 4_2:+2935058 Msa0584020:CDS
GATTCATGCAACAAGAGAAA+TGG 0.383099 4_2:+2937599 Msa0584020:CDS
TAAATGTGAAGTCAACTTAT+GGG 0.390794 4_2:+2936368 Msa0584020:intron
TACCAACATAGCTGCAGTTT+AGG 0.411492 4_2:-2936446 None:intergenic
CTTATGGGATCAAGAGAAAC+TGG 0.413837 4_2:+2936383 Msa0584020:CDS
GAAGATACAAAGGAATGAAT+AGG 0.428109 4_2:-2934947 None:intergenic
TTTGAAAAGGTTGTGGAGAA+AGG 0.428970 4_2:+2938016 Msa0584020:CDS
CAGAAACTTTGAAAAGGTTG+TGG 0.430911 4_2:+2938009 Msa0584020:CDS
ATATTCTATTGACTTCAGAT+TGG 0.437899 4_2:-2936607 None:intergenic
CCACCAACTGATACTACTAA+TGG 0.439249 4_2:-2937638 None:intergenic
CTAAGCTGCCAGTGACAACT+TGG 0.444235 4_2:+2934791 None:intergenic
TAATTGGAACTATAGCTTCT+GGG 0.444237 4_2:+2936580 Msa0584020:CDS
GTGAAACGAAGTTGGAACTT+TGG 0.448803 4_2:+2934997 Msa0584020:CDS
GTTGTGGAGAAAGGAGCATC+TGG 0.469808 4_2:+2938025 Msa0584020:CDS
CTTATAGGGTATTGTGATGA+TGG 0.472954 4_2:+2938302 Msa0584020:CDS
ATTCATGCAACAAGAGAAAT+GGG 0.480576 4_2:+2937600 Msa0584020:CDS
GGCCTAAACTGCAGCTATGT+TGG 0.481031 4_2:+2936444 Msa0584020:CDS
GGCATTATACTTGGGAAGGA+AGG 0.485026 4_2:-2934820 None:intergenic
CAACAAGAGAAATGGGAGTA+AGG 0.487221 4_2:+2937607 Msa0584020:CDS
ATATGTGGAGGCATTATACT+TGG 0.487647 4_2:-2934829 None:intergenic
CTGGAACAGTCTACCATGGC+TGG 0.488403 4_2:+2938044 Msa0584020:CDS
GGAAAATTCCAAGTTGTCAC+TGG 0.491907 4_2:-2934799 None:intergenic
AGTTATTAGTGAAACGAAGT+TGG 0.493413 4_2:+2934989 Msa0584020:CDS
GTACTCCAGTGTCTTACTTG+TGG 0.508313 4_2:+2936418 Msa0584020:CDS
CATTACCAGAAACTTTGAAA+AGG 0.512003 4_2:+2938003 Msa0584020:CDS
TCTCTTGTTGCATGAATCTG+AGG 0.513564 4_2:-2937594 None:intergenic
ACTTACAAGTATATGATCCT+TGG 0.514030 4_2:-2936477 None:intergenic
AAGTAAGACACTGGAGTACA+AGG 0.521279 4_2:-2936414 None:intergenic
ACTCTAGATTAGAGCCATGT+TGG 0.527280 4_2:-2938327 None:intergenic
GTTCATCATAAATACTTGAC+AGG 0.530148 4_2:+2938278 Msa0584020:CDS
CCAGTGTCTTACTTGTGGAA+TGG 0.545016 4_2:+2936423 Msa0584020:CDS
TAATAACTGATATATAATAC+AGG 0.545081 4_2:-2934974 None:intergenic
ATAGAAAGCCAAACCTACCT+TGG 0.547821 4_2:-2937718 None:intergenic
GTCTCGACGACATTGAATAG+TGG 0.551109 4_2:-2935050 None:intergenic
GTTCCATTAGTAGTATCAGT+TGG 0.554832 4_2:+2937635 Msa0584020:CDS
CAGATATCCTTGAGTTGATG+AGG 0.561128 4_2:-2938107 None:intergenic
GTGCATGTACACATAATATG+TGG 0.566614 4_2:-2934844 None:intergenic
CCATTAGTAGTATCAGTTGG+TGG 0.569905 4_2:+2937638 Msa0584020:CDS
TGGGATCAAGAGAAACTGGC+AGG 0.578914 4_2:+2936387 Msa0584020:CDS
CTATCTTCCTCATCAACTCA+AGG 0.579253 4_2:+2938100 Msa0584020:CDS
TGGAGGCATTATACTTGGGA+AGG 0.584867 4_2:-2934824 None:intergenic
AAACTGCAGCTATGTTGGTA+CGG 0.585202 4_2:+2936449 Msa0584020:CDS
TACAGGTTCTGAAGATACAA+AGG 0.605212 4_2:-2934957 None:intergenic
CCATTCCACAAGTAAGACAC+TGG 0.617935 4_2:-2936423 None:intergenic
CGTCGAGACATATATGGCAA+AGG 0.622385 4_2:+2935064 Msa0584020:CDS
TATGTGGAGGCATTATACTT+GGG 0.630511 4_2:-2934828 None:intergenic
TATCATGATCAATCCAGCCA+TGG 0.635701 4_2:-2938057 None:intergenic
TCAGAAACATATCAAACCGA+TGG 0.637155 4_2:+2935098 Msa0584020:CDS
TCGACGACATTGAATAGTGG+TGG 0.637303 4_2:-2935047 None:intergenic
ATATCTGCAATTTCAAGCAG+AGG 0.641697 4_2:+2938123 Msa0584020:CDS
CATGTACACATAATATGTGG+AGG 0.657421 4_2:-2934841 None:intergenic
GCATCTGGAACAGTCTACCA+TGG 0.666857 4_2:+2938040 Msa0584020:CDS
ATGTTGGTACGGATTCGCCA+AGG 0.677061 4_2:+2936460 Msa0584020:CDS
ACTACAAGAGACACCAAGGT+AGG 0.680841 4_2:+2937705 Msa0584020:CDS
CTGGATTGATCATGATACTG+AGG 0.708911 4_2:+2938063 Msa0584020:CDS
TTGTGATGATGGCACCAACA+TGG 0.719558 4_2:+2938313 Msa0584020:CDS
ATATACTACAAGAGACACCA+AGG 0.741720 4_2:+2937701 Msa0584020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTATTTTTATTAAA+TGG + chr4_2:2935387-2935406 Msa0584020:intron 0.0%
!!! ATTTTTATTTTTATTTTAAT+TGG - chr4_2:2936120-2936139 None:intergenic 0.0%
!!! AAAATAAAAAAACAGTTTTT+TGG + chr4_2:2935719-2935738 Msa0584020:intron 10.0%
!!! CTTAATTTTTTAAAAAGAAT+TGG + chr4_2:2935912-2935931 Msa0584020:intron 10.0%
!!! TAAATATTTTTCTGATTTTT+TGG + chr4_2:2936039-2936058 Msa0584020:intron 10.0%
!!! TTTTATTTCTTCTTTATATT+TGG + chr4_2:2937299-2937318 Msa0584020:intron 10.0%
!! ATTTATATATCAAAAATGTA+TGG + chr4_2:2937493-2937512 Msa0584020:intron 10.0%
!! ATAAAAATAGATGATTTAAT+TGG + chr4_2:2937773-2937792 Msa0584020:intron 10.0%
!! TAAAAATAGATGATTTAATT+GGG + chr4_2:2937774-2937793 Msa0584020:intron 10.0%
!! TAATAACTGATATATAATAC+AGG - chr4_2:2934977-2934996 None:intergenic 15.0%
!! TTTAAAAATAAAATCTGATG+TGG - chr4_2:2935433-2935452 None:intergenic 15.0%
!!! ATTTTTCTACTAAAAAAATC+TGG - chr4_2:2935698-2935717 None:intergenic 15.0%
!!! TACTTCGTTTTTTTTAAATT+AGG - chr4_2:2935826-2935845 None:intergenic 15.0%
!!! TCTATTTTTATTCTATTGTT+CGG - chr4_2:2937764-2937783 None:intergenic 15.0%
!! ATAATTTGACAATTTATGAT+CGG + chr4_2:2937902-2937921 Msa0584020:intron 15.0%
!!! AGTTCAACATTTTTGTAAAT+GGG + chr4_2:2934907-2934926 Msa0584020:CDS 20.0%
!! TCTGAAAAAAAAAATTTACG+AGG - chr4_2:2935310-2935329 None:intergenic 20.0%
!!! GGAAAAAAAATAATTTTACG+AGG + chr4_2:2936205-2936224 Msa0584020:intron 20.0%
!! TCAATTAATTATATTGCTTG+AGG + chr4_2:2936825-2936844 Msa0584020:intron 20.0%
!!! TTTTATTTACTGAAGAAACT+TGG + chr4_2:2936900-2936919 Msa0584020:intron 20.0%
!! AAAACTATACAAATTATGCA+AGG - chr4_2:2937003-2937022 None:intergenic 20.0%
!! TAGCCAAGTAATATATTAAT+GGG - chr4_2:2937039-2937058 None:intergenic 20.0%
!! AACTTTAAATAGAACATACT+TGG - chr4_2:2937418-2937437 None:intergenic 20.0%
!! GAGTTCAACATTTTTGTAAA+TGG + chr4_2:2934906-2934925 Msa0584020:CDS 25.0%
! AACTAAATAGTACATACCAT+CGG - chr4_2:2935117-2935136 None:intergenic 25.0%
! TTCTTACAATCTTATTCGTA+TGG + chr4_2:2935213-2935232 Msa0584020:intron 25.0%
! TCTTACAATCTTATTCGTAT+GGG + chr4_2:2935214-2935233 Msa0584020:intron 25.0%
! AGGTAAGACATTTAAAAATC+TGG - chr4_2:2935806-2935825 None:intergenic 25.0%
! GAAATAACCTATATTACAGA+GGG + chr4_2:2936266-2936285 Msa0584020:intron 25.0%
! TAGCATCAACTATTATCATA+AGG - chr4_2:2936345-2936364 None:intergenic 25.0%
! TAAATGTGAAGTCAACTTAT+GGG + chr4_2:2936368-2936387 Msa0584020:intron 25.0%
! TCATATACTTGTAAGTTATG+TGG + chr4_2:2936484-2936503 Msa0584020:intron 25.0%
!!! ATATTCTATTGACTTCAGAT+TGG - chr4_2:2936610-2936629 None:intergenic 25.0%
!!! CAAGGTTTATTTTTCTTTTG+GGG - chr4_2:2936985-2937004 None:intergenic 25.0%
!!! GCAAGGTTTATTTTTCTTTT+GGG - chr4_2:2936986-2937005 None:intergenic 25.0%
!!! TGCAAGGTTTATTTTTCTTT+TGG - chr4_2:2936987-2937006 None:intergenic 25.0%
! TACCCCATTAATATATTACT+TGG + chr4_2:2937033-2937052 Msa0584020:intron 25.0%
! AGCCAAGTAATATATTAATG+GGG - chr4_2:2937038-2937057 None:intergenic 25.0%
! GTAGCCAAGTAATATATTAA+TGG - chr4_2:2937040-2937059 None:intergenic 25.0%
!! TTGTCGCTTCATTAAATATA+AGG + chr4_2:2937213-2937232 Msa0584020:intron 25.0%
!! AGCTAATACAATTTTTACGA+GGG - chr4_2:2937327-2937346 None:intergenic 25.0%
!! AAGCTAATACAATTTTTACG+AGG - chr4_2:2937328-2937347 None:intergenic 25.0%
!!! GTGTGATTTTTAAGATAATC+AGG + chr4_2:2937857-2937876 Msa0584020:intron 25.0%
! ACAATTTATGATCGGTAAAT+TGG + chr4_2:2937910-2937929 Msa0584020:intron 25.0%
GAAGATACAAAGGAATGAAT+AGG - chr4_2:2934950-2934969 None:intergenic 30.0%
AGTTATTAGTGAAACGAAGT+TGG + chr4_2:2934989-2935008 Msa0584020:CDS 30.0%
CATGTAAATAATGAAGTGCA+TGG - chr4_2:2935188-2935207 None:intergenic 30.0%
CATGCACTTCATTATTTACA+TGG + chr4_2:2935186-2935205 Msa0584020:intron 30.0%
ACCAAACCGAAAATTTCTTA+GGG - chr4_2:2935281-2935300 None:intergenic 30.0%
AACCAAACCGAAAATTTCTT+AGG - chr4_2:2936233-2936252 None:intergenic 30.0%
TGGAAAATTAATTTCACACG+TGG - chr4_2:2935413-2935432 None:intergenic 30.0%
! TGTAAAATGTTTTTCACACG+TGG + chr4_2:2936094-2936113 Msa0584020:intron 30.0%
AAACCAAACCGAAAATTTCT+TGG + chr4_2:2936232-2936251 Msa0584020:intron 30.0%
AACCAAACCGAAAATTTCTT+GGG + chr4_2:2936233-2936252 Msa0584020:intron 30.0%
GGAAATAACCTATATTACAG+AGG + chr4_2:2936265-2936284 Msa0584020:intron 30.0%
!!! GTTTTTTCCCTCTGTAATAT+AGG - chr4_2:2936276-2936295 None:intergenic 30.0%
CTATACAGTGAATACGAATA+GGG - chr4_2:2936307-2936326 None:intergenic 30.0%
ACTATACAGTGAATACGAAT+AGG - chr4_2:2936308-2936327 None:intergenic 30.0%
! CATAAGTTGACTTCACATTT+AGG - chr4_2:2936369-2936388 None:intergenic 30.0%
CTAAATGTGAAGTCAACTTA+TGG + chr4_2:2936367-2936386 Msa0584020:intron 30.0%
ACTTACAAGTATATGATCCT+TGG - chr4_2:2936480-2936499 None:intergenic 30.0%
TTAATTGGAACTATAGCTTC+TGG + chr4_2:2936579-2936598 Msa0584020:CDS 30.0%
TAATTGGAACTATAGCTTCT+GGG + chr4_2:2936580-2936599 Msa0584020:CDS 30.0%
TTGTCGAATAACAATTTGAC+TGG + chr4_2:2936710-2936729 Msa0584020:intron 30.0%
ATCTTCATCTAAGTTATCCT+AGG + chr4_2:2936789-2936808 Msa0584020:intron 30.0%
ATTTACTGAAGAAACTTGGA+AGG + chr4_2:2936904-2936923 Msa0584020:intron 30.0%
! GCTACTCTAAACAATTTTGT+TGG + chr4_2:2937055-2937074 Msa0584020:intron 30.0%
! CTAATACAATTTTTACGAGG+GGG - chr4_2:2937325-2937344 None:intergenic 30.0%
! GCTAATACAATTTTTACGAG+GGG - chr4_2:2937326-2937345 None:intergenic 30.0%
! ATTACTGTATGTGCAAGTTT+TGG + chr4_2:2937551-2937570 Msa0584020:intron 30.0%
ATTCATGCAACAAGAGAAAT+GGG + chr4_2:2937600-2937619 Msa0584020:CDS 30.0%
! CATTACCAGAAACTTTGAAA+AGG + chr4_2:2938003-2938022 Msa0584020:CDS 30.0%
ATGAACTACAGCAAAAAGTT+TGG - chr4_2:2938264-2938283 None:intergenic 30.0%
GTTCATCATAAATACTTGAC+AGG + chr4_2:2938278-2938297 Msa0584020:CDS 30.0%
! AAATACTTGACAGGTCTTAT+AGG + chr4_2:2938287-2938306 Msa0584020:CDS 30.0%
! AATACTTGACAGGTCTTATA+GGG + chr4_2:2938288-2938307 Msa0584020:CDS 30.0%
!! TATGTGGAGGCATTATACTT+GGG - chr4_2:2934831-2934850 None:intergenic 35.0%
!! ATATGTGGAGGCATTATACT+TGG - chr4_2:2934832-2934851 None:intergenic 35.0%
CATGTACACATAATATGTGG+AGG - chr4_2:2934844-2934863 None:intergenic 35.0%
GTGCATGTACACATAATATG+TGG - chr4_2:2934847-2934866 None:intergenic 35.0%
!! TACAGGTTCTGAAGATACAA+AGG - chr4_2:2934960-2934979 None:intergenic 35.0%
TCAGAAACATATCAAACCGA+TGG + chr4_2:2935098-2935117 Msa0584020:CDS 35.0%
! TTCCTTTTGTAGTCATTTCC+AGG + chr4_2:2935249-2935268 Msa0584020:intron 35.0%
AACCTGGAAATGACTACAAA+AGG - chr4_2:2935254-2935273 None:intergenic 35.0%
AAACCGAAAATTTCTTAGGG+GGG - chr4_2:2935278-2935297 None:intergenic 35.0%
CAAACCGAAAATTTCTTAGG+GGG - chr4_2:2935279-2935298 None:intergenic 35.0%
CCAAACCGAAAATTTCTTAG+GGG - chr4_2:2935280-2935299 None:intergenic 35.0%
!! TACATTTTGCTGAGTTGGAT+GGG - chr4_2:2936168-2936187 None:intergenic 35.0%
!! TTACATTTTGCTGAGTTGGA+TGG - chr4_2:2936167-2936186 None:intergenic 35.0%
! CGTATTACATTTTGCTGAGT+TGG - chr4_2:2936163-2936182 None:intergenic 35.0%
ACTCAGCAAAATGTAATACG+TGG + chr4_2:2935350-2935369 Msa0584020:intron 35.0%
ACTCAGCAAAATGTAATACG+TGG - chr4_2:2935350-2935369 None:intergenic 35.0%
! CGTATTACATTTTGCTGAGT+TGG + chr4_2:2936163-2936182 Msa0584020:intron 35.0%
!! TTACATTTTGCTGAGTTGGA+TGG + chr4_2:2936167-2936186 Msa0584020:intron 35.0%
!! TACATTTTGCTGAGTTGGAT+GGG + chr4_2:2936168-2936187 Msa0584020:intron 35.0%
! GTTTCCTCCCAAGAAATTTT+CGG - chr4_2:2936243-2936262 None:intergenic 35.0%
AAATTTCTTGGGAGGAAACT+TGG + chr4_2:2936244-2936263 Msa0584020:intron 35.0%
AGTTCCTGAAGGATTATCAT+CGG - chr4_2:2936530-2936549 None:intergenic 35.0%
TTAACTTCCAGCGGTTTAAT+TGG + chr4_2:2936564-2936583 Msa0584020:intron 35.0%
TATAGTTCCAATTAAACCGC+TGG - chr4_2:2936574-2936593 None:intergenic 35.0%
TTGAATAATCAAGTCAGCCT+AGG - chr4_2:2936809-2936828 None:intergenic 35.0%
TACTATTCTTTGAACGCACA+AGG - chr4_2:2937386-2937405 None:intergenic 35.0%
ACTAACACATCACGTACAAA+AGG - chr4_2:2937463-2937482 None:intergenic 35.0%
GATTCATGCAACAAGAGAAA+TGG + chr4_2:2937599-2937618 Msa0584020:CDS 35.0%
GTTCCATTAGTAGTATCAGT+TGG + chr4_2:2937635-2937654 Msa0584020:CDS 35.0%
ATATACTACAAGAGACACCA+AGG + chr4_2:2937701-2937720 Msa0584020:CDS 35.0%
GCTTTCTATTCCATTGACAT+TGG + chr4_2:2937732-2937751 Msa0584020:intron 35.0%
TCTATTCCATTGACATTGGT+AGG + chr4_2:2937736-2937755 Msa0584020:intron 35.0%
! CACAACCTTTTCAAAGTTTC+TGG - chr4_2:2938011-2938030 None:intergenic 35.0%
! CAGAAACTTTGAAAAGGTTG+TGG + chr4_2:2938009-2938028 Msa0584020:CDS 35.0%
TTTGAAAAGGTTGTGGAGAA+AGG + chr4_2:2938016-2938035 Msa0584020:CDS 35.0%
ATATCTGCAATTTCAAGCAG+AGG + chr4_2:2938123-2938142 Msa0584020:CDS 35.0%
CTTGTGAACTGAAGGAATTA+TGG + chr4_2:2938217-2938236 Msa0584020:intron 35.0%
!! CTTATAGGGTATTGTGATGA+TGG + chr4_2:2938302-2938321 Msa0584020:CDS 35.0%
GGAAAATTCCAAGTTGTCAC+TGG - chr4_2:2934802-2934821 None:intergenic 40.0%
!! TTTGATTTGCATCGAGCTTC+TGG - chr4_2:2934881-2934900 None:intergenic 40.0%
GTGAAACGAAGTTGGAACTT+TGG + chr4_2:2934997-2935016 Msa0584020:CDS 40.0%
CAATGTCGTCGAGACATATA+TGG + chr4_2:2935058-2935077 Msa0584020:CDS 40.0%
! TTCCCCCCCTAAGAAATTTT+CGG + chr4_2:2935272-2935291 Msa0584020:intron 40.0%
AACCGAAAATTTCTTAGGGG+GGG - chr4_2:2935277-2935296 None:intergenic 40.0%
! CCCCTAAGAAATTTTCGGTT+TGG + chr4_2:2935277-2935296 Msa0584020:intron 40.0%
!! ACATTTTGCTGAGTTGGATG+GGG - chr4_2:2936169-2936188 None:intergenic 40.0%
!! ACATTTTGCTGAGTTGGATG+GGG + chr4_2:2936169-2936188 Msa0584020:intron 40.0%
! CTCCCAAGAAATTTTCGGTT+TGG - chr4_2:2936238-2936257 None:intergenic 40.0%
CAAACCGAAAATTTCTTGGG+AGG + chr4_2:2936236-2936255 Msa0584020:intron 40.0%
CTTATGGGATCAAGAGAAAC+TGG + chr4_2:2936383-2936402 Msa0584020:CDS 40.0%
AAGTAAGACACTGGAGTACA+AGG - chr4_2:2936417-2936436 None:intergenic 40.0%
TACCAACATAGCTGCAGTTT+AGG - chr4_2:2936449-2936468 None:intergenic 40.0%
AAACTGCAGCTATGTTGGTA+CGG + chr4_2:2936449-2936468 Msa0584020:CDS 40.0%
!! TTCTGGAACTTAACTTCCAG+CGG + chr4_2:2936555-2936574 Msa0584020:intron 40.0%
AAGGAAATCAGCTATCAGCA+GGG + chr4_2:2936923-2936942 Msa0584020:intron 40.0%
GCGAATCCCTTCATAACAAT+GGG + chr4_2:2936946-2936965 Msa0584020:intron 40.0%
CGAATCCCTTCATAACAATG+GGG + chr4_2:2936947-2936966 Msa0584020:intron 40.0%
TGCTACCCCATTGTTATGAA+GGG - chr4_2:2936955-2936974 None:intergenic 40.0%
ATGCTACCCCATTGTTATGA+AGG - chr4_2:2936956-2936975 None:intergenic 40.0%
TCATAACAATGGGGTAGCAT+TGG + chr4_2:2936956-2936975 Msa0584020:intron 40.0%
TCTCTTGTTGCATGAATCTG+AGG - chr4_2:2937597-2937616 None:intergenic 40.0%
CAACAAGAGAAATGGGAGTA+AGG + chr4_2:2937607-2937626 Msa0584020:CDS 40.0%
CCACCAACTGATACTACTAA+TGG - chr4_2:2937641-2937660 None:intergenic 40.0%
CCATTAGTAGTATCAGTTGG+TGG + chr4_2:2937638-2937657 Msa0584020:CDS 40.0%
ATAGAAAGCCAAACCTACCT+TGG - chr4_2:2937721-2937740 None:intergenic 40.0%
TATCATGATCAATCCAGCCA+TGG - chr4_2:2938060-2938079 None:intergenic 40.0%
CTGGATTGATCATGATACTG+AGG + chr4_2:2938063-2938082 Msa0584020:CDS 40.0%
CTATCTTCCTCATCAACTCA+AGG + chr4_2:2938100-2938119 Msa0584020:CDS 40.0%
!! CAGATATCCTTGAGTTGATG+AGG - chr4_2:2938110-2938129 None:intergenic 40.0%
ACGATGTTCTTGTGAACTGA+AGG + chr4_2:2938209-2938228 Msa0584020:intron 40.0%
! GGTTTTCCTTTCTGATTCAC+AGG + chr4_2:2938238-2938257 Msa0584020:intron 40.0%
GGCATTATACTTGGGAAGGA+AGG - chr4_2:2934823-2934842 None:intergenic 45.0%
!! TGGAGGCATTATACTTGGGA+AGG - chr4_2:2934827-2934846 None:intergenic 45.0%
TCGACGACATTGAATAGTGG+TGG - chr4_2:2935050-2935069 None:intergenic 45.0%
GTCTCGACGACATTGAATAG+TGG - chr4_2:2935053-2935072 None:intergenic 45.0%
CGTCGAGACATATATGGCAA+AGG + chr4_2:2935064-2935083 Msa0584020:CDS 45.0%
!! CATTTTGCTGAGTTGGATGG+GGG - chr4_2:2936170-2936189 None:intergenic 45.0%
!! TGTAATACGTGGCGCTGATT+TGG + chr4_2:2935361-2935380 Msa0584020:intron 45.0%
GTACTCCAGTGTCTTACTTG+TGG + chr4_2:2936418-2936437 Msa0584020:CDS 45.0%
CCATTCCACAAGTAAGACAC+TGG - chr4_2:2936426-2936445 None:intergenic 45.0%
CCAGTGTCTTACTTGTGGAA+TGG + chr4_2:2936423-2936442 Msa0584020:CDS 45.0%
TACGCCGATGATAATCCTTC+AGG + chr4_2:2936523-2936542 Msa0584020:intron 45.0%
CCAGAAGCTATAGTTCCTGA+AGG - chr4_2:2936541-2936560 None:intergenic 45.0%
CCTTCAGGAACTATAGCTTC+TGG + chr4_2:2936538-2936557 Msa0584020:intron 45.0%
CGTAGCTGAGATAGGAAATC+AGG - chr4_2:2936743-2936762 None:intergenic 45.0%
! TCAAGAAGCGTAGCTGAGAT+AGG - chr4_2:2936751-2936770 None:intergenic 45.0%
GAAGGAAATCAGCTATCAGC+AGG + chr4_2:2936922-2936941 Msa0584020:intron 45.0%
AGGAAATCAGCTATCAGCAG+GGG + chr4_2:2936924-2936943 Msa0584020:intron 45.0%
GGCGAATCCCTTCATAACAA+TGG + chr4_2:2936945-2936964 Msa0584020:intron 45.0%
! AATTGTATTAGCTTCGCCGC+TGG + chr4_2:2937334-2937353 Msa0584020:intron 45.0%
GAATTGATCCTGATAGCCAG+CGG - chr4_2:2937353-2937372 None:intergenic 45.0%
GAACGCACAAGGAGTTGTAT+TGG - chr4_2:2937375-2937394 None:intergenic 45.0%
ACTACAAGAGACACCAAGGT+AGG + chr4_2:2937705-2937724 Msa0584020:CDS 45.0%
AAGAGACACCAAGGTAGGTT+TGG + chr4_2:2937710-2937729 Msa0584020:intron 45.0%
GTTTGGCCTGTGAATCAGAA+AGG - chr4_2:2938247-2938266 None:intergenic 45.0%
!! TTGTGATGATGGCACCAACA+TGG + chr4_2:2938313-2938332 Msa0584020:CDS 45.0%
!!! TTTAATATTTTTTTAGTTTT+GGG - chr4_2:2935490-2935509 None:intergenic 5.0%
!!! TTTTAATATTTTTTTAGTTT+TGG - chr4_2:2935491-2935510 None:intergenic 5.0%
!!! TTAATTTTTTAAAAAGAATT+GGG + chr4_2:2935913-2935932 Msa0584020:intron 5.0%
AATTTCTTAGGGGGGGAACC+TGG - chr4_2:2935270-2935289 None:intergenic 50.0%
! TTTGCTGAGTTGGATGGGGT+AGG + chr4_2:2936173-2936192 Msa0584020:intron 50.0%
! TTGCTGAGTTGGATGGGGTA+GGG + chr4_2:2936174-2936193 Msa0584020:intron 50.0%
TGGGATCAAGAGAAACTGGC+AGG + chr4_2:2936387-2936406 Msa0584020:CDS 50.0%
GGCCTAAACTGCAGCTATGT+TGG + chr4_2:2936444-2936463 Msa0584020:CDS 50.0%
ATGTTGGTACGGATTCGCCA+AGG + chr4_2:2936460-2936479 Msa0584020:CDS 50.0%
TCGGCTCCTACCAATGTCAA+TGG - chr4_2:2937745-2937764 None:intergenic 50.0%
!! GTTGTGGAGAAAGGAGCATC+TGG + chr4_2:2938025-2938044 Msa0584020:CDS 50.0%
! GCATCTGGAACAGTCTACCA+TGG + chr4_2:2938040-2938059 Msa0584020:CDS 50.0%
! TTGCTGAGTTGGATGGGGGA+GGG - chr4_2:2935339-2935358 None:intergenic 55.0%
! TTTGCTGAGTTGGATGGGGG+AGG - chr4_2:2935340-2935359 None:intergenic 55.0%
! TGCTGAGTTGGATGGGGTAG+GGG + chr4_2:2936175-2936194 Msa0584020:intron 55.0%
!!! GGATGGGGTAGGGGGTATTT+TGG + chr4_2:2936184-2936203 Msa0584020:intron 55.0%
CTGGAACAGTCTACCATGGC+TGG + chr4_2:2938044-2938063 Msa0584020:CDS 55.0%
! TGCTGAGTTGGATGGGGGAG+GGG - chr4_2:2935338-2935357 None:intergenic 60.0%
! GCTGAGTTGGATGGGGTAGG+GGG + chr4_2:2936176-2936195 Msa0584020:intron 60.0%
! TAGCTTCGCCGCTGGCTATC+AGG + chr4_2:2937342-2937361 Msa0584020:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 2934798 2938346 2934798 ID=Msa0584020;Name=Msa0584020
chr4_2 mRNA 2934798 2938346 2934798 ID=Msa0584020-mRNA-1;Parent=Msa0584020;Name=Msa0584020-mRNA-1;_AED=0.18;_eAED=0.21;_QI=0|0|0|1|0.6|0.83|6|0|299
chr4_2 exon 2934798 2935119 2934798 ID=Msa0584020-mRNA-1:exon:837;Parent=Msa0584020-mRNA-1
chr4_2 exon 2936379 2936493 2936379 ID=Msa0584020-mRNA-1:exon:838;Parent=Msa0584020-mRNA-1
chr4_2 exon 2936575 2936631 2936575 ID=Msa0584020-mRNA-1:exon:839;Parent=Msa0584020-mRNA-1
chr4_2 exon 2937568 2937726 2937568 ID=Msa0584020-mRNA-1:exon:840;Parent=Msa0584020-mRNA-1
chr4_2 exon 2937985 2938144 2937985 ID=Msa0584020-mRNA-1:exon:841;Parent=Msa0584020-mRNA-1
chr4_2 exon 2938260 2938346 2938260 ID=Msa0584020-mRNA-1:exon:842;Parent=Msa0584020-mRNA-1
chr4_2 CDS 2934798 2935119 2934798 ID=Msa0584020-mRNA-1:cds;Parent=Msa0584020-mRNA-1
chr4_2 CDS 2936379 2936493 2936379 ID=Msa0584020-mRNA-1:cds;Parent=Msa0584020-mRNA-1
chr4_2 CDS 2936575 2936631 2936575 ID=Msa0584020-mRNA-1:cds;Parent=Msa0584020-mRNA-1
chr4_2 CDS 2937568 2937726 2937568 ID=Msa0584020-mRNA-1:cds;Parent=Msa0584020-mRNA-1
chr4_2 CDS 2937985 2938144 2937985 ID=Msa0584020-mRNA-1:cds;Parent=Msa0584020-mRNA-1
chr4_2 CDS 2938260 2938346 2938260 ID=Msa0584020-mRNA-1:cds;Parent=Msa0584020-mRNA-1
Gene Sequence

>Msa0584020

GCCAGTGACAACTTGGAATTTTCCTTCCTTCCCAAGTATAATGCCTCCACATATTATGTGTACATGCACTTTGCTGAAATCCAGAAGCTCGATGCAAATCAAATTAGAGAGTTCAACATTTTTGTAAATGGGCAGCTTATAAATAGTGACCTATTCATTCCTTTGTATCTTCAGAACCTGTATTATATATCAGTTATTAGTGAAACGAAGTTGGAACTTTGGTTTAATAAGACTAGTAGATCAACTCTTCCACCACTATTCAATGTCGTCGAGACATATATGGCAAAGGACTTCTTACAATCAGAAACATATCAAACCGATGTCAACTTATGGGATCAAGAGAAACTGGCAGGAGATCCTTGTACTCCAGTGTCTTACTTGTGGAATGGCCTAAACTGCAGCTATGTTGGTACGGATTCGCCAAGGATCATATACTTCGGTTTAATTGGAACTATAGCTTCTGGGATATCCAATCTGAAGTCAATAGAATATTTTTTTGGTAGAAATTCAAATCTTTGCACCTCAGATTCATGCAACAAGAGAAATGGGAGTAAGGTTTTAGTTCCATTAGTAGTATCAGTTGGTGGAGCTTTCATTATACTAGTAGTAGCAATGATTTCTTTTCGCATATACTACAAGAGACACCAAGGTAGCTATGAAGAAGTGTTAAACATTACCAGAAACTTTGAAAAGGTTGTGGAGAAAGGAGCATCTGGAACAGTCTACCATGGCTGGATTGATCATGATACTGAGGTAGCTGTCAAAATGCTATCTTCCTCATCAACTCAAGGATATCTGCAATTTCAAGCAGAGGCCAAACTTTTTGCTGTAGTTCATCATAAATACTTGACAGGTCTTATAGGGTATTGTGATGATGGCACCAACATGGCTCTAATCTAG
Protein sequence

>Msa0584020

ASDNLEFSFLPKYNASTYYVYMHFAEIQKLDANQIREFNIFVNGQLINSDLFIPLYLQNLYYISVISETKLELWFNKTSRSTLPPLFNVVETYMAKDFLQSETYQTDVNLWDQEKLAGDPCTPVSYLWNGLNCSYVGTDSPRIIYFGLIGTIASGISNLKSIEYFFGRNSNLCTSDSCNKRNGSKVLVPLVVSVGGAFIILVVAMISFRIYYKRHQGSYEEVLNITRNFEKVVEKGASGTVYHGWIDHDTEVAVKMLSSSSTQGYLQFQAEAKLFAVVHHKYLTGLIGYCDDGTNMALI*