AlfalfaGEDB | Msa0584770

M. sativa cultivar zhongmu-4 / Msa0584770

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0584770	AES87959.1	83.761	117	18	1	1	116	1	117	1.41e-57	184

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
Msa0584770	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0584770	MtrunA17_Chr4g0019481	83.761	117	18	1	1	116	1	117	1.29e-61	184
Msa0584770	MtrunA17_Chr4g0019471	74.809	131	18	2	1	116	1	131	6.39e-57	172
Msa0584770	MtrunA17_Chr4g0019551	77.778	117	25	1	1	116	1	117	3.38e-48	150

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 17 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTCAAATGGTTGCACATG+TGG	0.522527	4_2:-4758019	None:intergenic
AATGGTTGCACATGTGGAGG+TGG	0.555990	4_2:-4758013	None:intergenic
ACTCGATTCTAATATAAAAT+CGG	0.323743	4_2:-4757840	None:intergenic
ACTGCTAAAGCTTCACGAGA+TGG	0.614779	4_2:-4757794	None:intergenic
AGGTCGAAAGCTCGAACAGG+AGG	0.636300	4_2:-4758049	None:intergenic
ATATTAGAATCGAGTAAACA+AGG	0.624706	4_2:+4757848	Msa0584770:CDS
CGAAAGCTCGAACAGGAGGT+TGG	0.564077	4_2:-4758045	None:intergenic
CTCATTCATCTGGTGTATAA+GGG	0.375685	4_2:-4758078	None:intergenic
CTCGATTCTAATATAAAATC+GGG	0.421980	4_2:-4757839	None:intergenic
CTGGTGTATAAGGGAGATTG+AGG	0.611261	4_2:-4758069	None:intergenic

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CRISPR-GE

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badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGTCAAATGGTTGCACATG+TGG	-	chr4_2:4758022-4758041	None:intergenic	40.0%
	ACTGCTAAAGCTTCACGAGA+TGG	-	chr4_2:4757797-4757816	None:intergenic	45.0%
	TCAAATGGTTGCACATGTGG+AGG	-	chr4_2:4758019-4758038	None:intergenic	45.0%
	CTGGTGTATAAGGGAGATTG+AGG	-	chr4_2:4758072-4758091	None:intergenic	45.0%
	AATGGTTGCACATGTGGAGG+TGG	-	chr4_2:4758016-4758035	None:intergenic	50.0%
	TTGAGGTCGAAAGCTCGAAC+AGG	-	chr4_2:4758055-4758074	None:intergenic	50.0%
	CGAAAGCTCGAACAGGAGGT+TGG	-	chr4_2:4758048-4758067	None:intergenic	55.0%
	AGGTCGAAAGCTCGAACAGG+AGG	-	chr4_2:4758052-4758071	None:intergenic	55.0%
!	CTCGATTCTAATATAAAATC+GGG	-	chr4_2:4757842-4757861	None:intergenic	25.0%
!	ATATTAGAATCGAGTAAACA+AGG	+	chr4_2:4757848-4757867	Msa0584770:CDS	25.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	4757749	4758099	4757749	ID=Msa0584770;Name=Msa0584770
chr4_2	mRNA	4757749	4758099	4757749	ID=Msa0584770-mRNA-1;Parent=Msa0584770;Name=Msa0584770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|116
chr4_2	exon	4757749	4758099	4757749	ID=Msa0584770-mRNA-1:exon:1196;Parent=Msa0584770-mRNA-1
chr4_2	CDS	4757749	4758099	4757749	ID=Msa0584770-mRNA-1:cds;Parent=Msa0584770-mRNA-1

Gene Sequence	>Msa0584770 ATGTCAAAGCTAGAATTCTTATGCAGTGAATGTGACACAATGTTTCCATCTCGTGAAGCTTTAGCAGTGCATGTTATCGAAGAACATCATCCCGATTTTATATTAGAATCGAGTAAACAAGGTCCACCACCAAATAACCAGCCCGAACCAGAAAATGCTCAGCCTCAACCACAAAATGTTGAACATCAACCACAAAATGCACTAGCTCCACCACAAAATGTTGAACAACAACCACAAAATGCACCAGCTCCACCACAAAATGCACCACCTCCACATGTGCAACCATTTGACTTGAACCAACCTCCTGTTCGAGCTTTCGACCTCAATCTCCCTTATACACCAGATGAATGA
Protein sequence	>Msa0584770 MSKLEFLCSECDTMFPSREALAVHVIEEHHPDFILESSKQGPPPNNQPEPENAQPQPQNVEHQPQNALAPPQNVEQQPQNAPAPPQNAPPPHVQPFDLNQPPVRAFDLNLPYTPDE*