Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0584770 | AES87959.1 | 83.761 | 117 | 18 | 1 | 1 | 116 | 1 | 117 | 1.41e-57 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0584770 | TF | C2H2 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0584770 | MtrunA17_Chr4g0019481 | 83.761 | 117 | 18 | 1 | 1 | 116 | 1 | 117 | 1.29e-61 | 184 |
| Msa0584770 | MtrunA17_Chr4g0019471 | 74.809 | 131 | 18 | 2 | 1 | 116 | 1 | 131 | 6.39e-57 | 172 |
| Msa0584770 | MtrunA17_Chr4g0019551 | 77.778 | 117 | 25 | 1 | 1 | 116 | 1 | 117 | 3.38e-48 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGGTTCGGGCTGGTTATT+TGG | 0.202950 | 4_2:-4757878 | None:intergenic |
| TCTCATTCATCTGGTGTATA+AGG | 0.203991 | 4_2:-4758079 | None:intergenic |
| ACTCGATTCTAATATAAAAT+CGG | 0.323743 | 4_2:-4757840 | None:intergenic |
| CTCATTCATCTGGTGTATAA+GGG | 0.375685 | 4_2:-4758078 | None:intergenic |
| CTCGATTCTAATATAAAATC+GGG | 0.421980 | 4_2:-4757839 | None:intergenic |
| GGAGGTTGGTTCAAGTCAAA+TGG | 0.461699 | 4_2:-4758031 | None:intergenic |
| TTGAGGTCGAAAGCTCGAAC+AGG | 0.496472 | 4_2:-4758052 | None:intergenic |
| TCGGGCTGGTTATTTGGTGG+TGG | 0.505201 | 4_2:-4757872 | None:intergenic |
| AAGTCAAATGGTTGCACATG+TGG | 0.522527 | 4_2:-4758019 | None:intergenic |
| AATGGTTGCACATGTGGAGG+TGG | 0.555990 | 4_2:-4758013 | None:intergenic |
| GGTTCGGGCTGGTTATTTGG+TGG | 0.558588 | 4_2:-4757875 | None:intergenic |
| CGAAAGCTCGAACAGGAGGT+TGG | 0.564077 | 4_2:-4758045 | None:intergenic |
| CTGGTGTATAAGGGAGATTG+AGG | 0.611261 | 4_2:-4758069 | None:intergenic |
| ACTGCTAAAGCTTCACGAGA+TGG | 0.614779 | 4_2:-4757794 | None:intergenic |
| ATATTAGAATCGAGTAAACA+AGG | 0.624706 | 4_2:+4757848 | Msa0584770:CDS |
| AGGTCGAAAGCTCGAACAGG+AGG | 0.636300 | 4_2:-4758049 | None:intergenic |
| TCAAATGGTTGCACATGTGG+AGG | 0.671560 | 4_2:-4758016 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACTCGATTCTAATATAAAAT+CGG | - | chr4_2:4757843-4757862 | None:intergenic | 20.0% |
| ! | CTCGATTCTAATATAAAATC+GGG | - | chr4_2:4757842-4757861 | None:intergenic | 25.0% |
| ! | ATATTAGAATCGAGTAAACA+AGG | + | chr4_2:4757848-4757867 | Msa0584770:CDS | 25.0% |
| ! | TGTTCAACATTTTGTGGTTG+AGG | - | chr4_2:4757914-4757933 | None:intergenic | 35.0% |
| !! | GGTTGATGTTCAACATTTTG+TGG | - | chr4_2:4757920-4757939 | None:intergenic | 35.0% |
| ! | TGTTGTTCAACATTTTGTGG+TGG | - | chr4_2:4757959-4757978 | None:intergenic | 35.0% |
| !! | GGTTGTTGTTCAACATTTTG+TGG | - | chr4_2:4757962-4757981 | None:intergenic | 35.0% |
| AAGTCAAATGGTTGCACATG+TGG | - | chr4_2:4758022-4758041 | None:intergenic | 40.0% | |
| ACTGCTAAAGCTTCACGAGA+TGG | - | chr4_2:4757797-4757816 | None:intergenic | 45.0% | |
| !!! | AGGCTGAGCATTTTCTGGTT+CGG | - | chr4_2:4757894-4757913 | None:intergenic | 45.0% |
| TCAAATGGTTGCACATGTGG+AGG | - | chr4_2:4758019-4758038 | None:intergenic | 45.0% | |
| ! | GGAGGTTGGTTCAAGTCAAA+TGG | - | chr4_2:4758034-4758053 | None:intergenic | 45.0% |
| CTGGTGTATAAGGGAGATTG+AGG | - | chr4_2:4758072-4758091 | None:intergenic | 45.0% | |
| !! | TCTGGTTCGGGCTGGTTATT+TGG | - | chr4_2:4757881-4757900 | None:intergenic | 50.0% |
| !!! | GGCTGAGCATTTTCTGGTTC+GGG | - | chr4_2:4757893-4757912 | None:intergenic | 50.0% |
| ! | GGTTGAGGCTGAGCATTTTC+TGG | - | chr4_2:4757899-4757918 | None:intergenic | 50.0% |
| ! | GGTGGAGCTAGTGCATTTTG+TGG | - | chr4_2:4757941-4757960 | None:intergenic | 50.0% |
| ! | TGTGGAGGTGGTGCATTTTG+TGG | - | chr4_2:4758004-4758023 | None:intergenic | 50.0% |
| AATGGTTGCACATGTGGAGG+TGG | - | chr4_2:4758016-4758035 | None:intergenic | 50.0% | |
| TTGAGGTCGAAAGCTCGAAC+AGG | - | chr4_2:4758055-4758074 | None:intergenic | 50.0% | |
| !! | TCGGGCTGGTTATTTGGTGG+TGG | - | chr4_2:4757875-4757894 | None:intergenic | 55.0% |
| !! | GGTTCGGGCTGGTTATTTGG+TGG | - | chr4_2:4757878-4757897 | None:intergenic | 55.0% |
| !!! | GAGCATTTTCTGGTTCGGGC+TGG | - | chr4_2:4757889-4757908 | None:intergenic | 55.0% |
| ! | GGTGGAGCTGGTGCATTTTG+TGG | - | chr4_2:4757983-4758002 | None:intergenic | 55.0% |
| !! | GGTGCATTTTGTGGTGGAGC+TGG | - | chr4_2:4757995-4758014 | None:intergenic | 55.0% |
| ! | GGAGGTGGTGCATTTTGTGG+TGG | - | chr4_2:4758001-4758020 | None:intergenic | 55.0% |
| CGAAAGCTCGAACAGGAGGT+TGG | - | chr4_2:4758048-4758067 | None:intergenic | 55.0% | |
| AGGTCGAAAGCTCGAACAGG+AGG | - | chr4_2:4758052-4758071 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 4757749 | 4758099 | 4757749 | ID=Msa0584770;Name=Msa0584770 |
| chr4_2 | mRNA | 4757749 | 4758099 | 4757749 | ID=Msa0584770-mRNA-1;Parent=Msa0584770;Name=Msa0584770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|116 |
| chr4_2 | exon | 4757749 | 4758099 | 4757749 | ID=Msa0584770-mRNA-1:exon:1196;Parent=Msa0584770-mRNA-1 |
| chr4_2 | CDS | 4757749 | 4758099 | 4757749 | ID=Msa0584770-mRNA-1:cds;Parent=Msa0584770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
