AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0585130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585130 MtrunA17_Chr4g0014321 77.778 99 22 0 1 99 1 99 2.28e-50 160
Msa0585130 MtrunA17_Chr4g0014321 53.247 77 36 0 76 152 155 231 3.06e-20 83.2
Msa0585130 MtrunA17_Chr4g0013041 72.727 99 25 1 1 99 1 97 3.82e-46 149
Msa0585130 MtrunA17_Chr8g0351751 63.366 101 35 1 1 99 1 101 1.32e-40 136
Msa0585130 MtrunA17_Chr2g0296831 60.185 108 43 0 1 108 1 108 8.33e-37 122
Msa0585130 MtrunA17_Chr3g0110131 61.616 99 38 0 1 99 1 99 4.23e-35 122
Msa0585130 MtrunA17_Chr3g0107381 51.515 99 48 0 1 99 1 99 1.18e-34 120
Msa0585130 MtrunA17_Chr4g0014031 62.626 99 37 0 1 99 1 99 1.80e-34 120
Msa0585130 MtrunA17_Chr4g0014121 65.657 99 34 0 1 99 29 127 9.66e-34 117
Msa0585130 MtrunA17_Chr3g0107331 50.000 96 48 0 4 99 3 98 3.14e-33 115
Msa0585130 MtrunA17_Chr4g0014131 60.606 99 39 0 1 99 1 99 3.54e-30 109
Msa0585130 MtrunA17_Chr5g0432691 47.917 96 50 0 1 96 1 96 1.14e-28 103
Msa0585130 MtrunA17_Chr2g0283941 46.939 98 52 0 1 98 1 98 1.44e-28 105
Msa0585130 MtrunA17_Chr3g0107361 44.211 95 53 0 5 99 4 98 4.01e-28 102
Msa0585130 MtrunA17_Chr3g0107341 45.263 95 52 0 5 99 4 98 4.66e-28 101
Msa0585130 MtrunA17_Chr3g0107351 41.121 107 63 0 5 111 4 110 1.13e-27 100
Msa0585130 MtrunA17_Chr3g0107461 43.617 94 53 0 1 94 1 94 1.14e-26 98.2
Msa0585130 MtrunA17_Chr5g0420351 37.391 115 71 1 1 115 1 114 1.24e-25 95.5
Msa0585130 MtrunA17_Chr1g0188681 42.424 99 56 1 1 99 1 98 6.43e-25 93.6
Msa0585130 MtrunA17_Chr1g0197261 38.596 114 69 1 1 114 1 113 5.84e-24 91.3
Msa0585130 MtrunA17_Chr1g0197321 39.394 99 59 1 1 99 1 98 5.93e-24 91.3
Msa0585130 MtrunA17_Chr1g0197461 42.708 96 54 1 4 99 3 97 1.06e-23 90.5
Msa0585130 MtrunA17_Chr2g0296861 35.789 95 61 0 1 95 1 95 8.96e-22 85.5
Msa0585130 MtrunA17_Chr7g0267601 41.053 95 56 0 4 98 7 101 6.62e-20 85.1
Msa0585130 MtrunA17_Chr4g0031421 49.275 69 35 0 29 97 1 69 9.36e-20 80.1
Msa0585130 MtrunA17_Chr4g0008701 38.947 95 58 0 4 98 7 101 2.21e-19 83.6
Msa0585130 MtrunA17_Chr3g0105791 47.826 69 36 0 29 97 1 69 6.82e-19 77.8
Msa0585130 MtrunA17_Chr1g0193311 41.414 99 57 1 1 99 1 98 1.13e-18 77.4
Msa0585130 MtrunA17_Chr7g0218151 38.384 99 60 1 1 99 1 98 5.53e-18 75.9
Msa0585130 MtrunA17_Chr3g0106501 42.254 71 41 0 29 99 1 71 1.10e-16 72.4
Msa0585130 MtrunA17_Chr1g0188661 37.374 99 61 1 1 99 1 98 1.74e-16 72.0
Msa0585130 MtrunA17_Chr3g0128721 36.667 90 57 0 4 93 7 96 6.57e-16 73.6
Msa0585130 MtrunA17_Chr4g0012761 38.384 99 60 1 1 99 1 98 1.17e-15 69.7
Msa0585130 MtrunA17_Chr1g0188651 40.404 99 58 1 1 99 1 98 1.57e-15 69.3
Msa0585130 MtrunA17_Chr3g0106421 40.000 85 49 1 29 111 1 85 8.91e-15 68.6
Msa0585130 MtrunA17_Chr1g0212151 36.000 100 57 3 5 98 5 103 9.69e-14 67.0
Msa0585130 MtrunA17_Chr6g0457851 37.000 100 56 4 5 98 15 113 7.15e-13 64.7
Msa0585130 MtrunA17_Chr6g0449861 36.000 100 57 3 5 98 13 111 1.50e-11 60.8
Msa0585130 MtrunA17_Chr6g0449851 37.000 100 56 3 5 98 5 103 2.23e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585130 AT5G48670.1 56.000 100 44 0 1 100 1 100 4.81e-29 108
Msa0585130 AT3G05860.2 39.062 128 71 2 1 128 1 121 2.66e-28 103
Msa0585130 AT3G05860.3 43.243 111 57 1 1 111 1 105 7.71e-28 103
Msa0585130 AT3G05860.1 43.243 111 57 1 1 111 1 105 1.02e-27 103
Msa0585130 AT5G27960.1 43.810 105 59 0 4 108 2 106 9.15e-26 100
Msa0585130 AT1G65300.1 47.917 96 50 0 4 99 3 98 9.16e-26 99.4
Msa0585130 AT5G26650.1 43.810 105 59 0 4 108 2 106 1.02e-25 100
Msa0585130 AT1G65330.1 46.739 92 49 0 5 96 4 95 4.98e-24 94.7
Msa0585130 AT5G27810.1 50.000 68 34 0 29 96 1 68 5.62e-20 80.1
Msa0585130 AT1G31640.1 40.000 95 56 1 5 99 4 97 7.92e-20 85.1
Msa0585130 AT5G26580.1 40.404 99 45 1 1 99 1 85 1.55e-19 83.6
Msa0585130 AT1G31630.1 40.217 92 54 1 5 96 4 94 2.46e-19 82.8
Msa0585130 AT5G06500.1 40.196 102 61 0 1 102 1 102 2.52e-19 81.6
Msa0585130 AT5G26630.1 40.000 115 69 0 1 115 1 115 2.89e-19 80.9
Msa0585130 AT2G28700.1 36.735 98 62 0 1 98 1 98 5.01e-19 82.0
Msa0585130 AT2G40210.1 37.374 99 62 0 1 99 1 99 3.99e-17 77.0
Msa0585130 AT1G22590.2 31.633 98 67 0 1 98 1 98 2.12e-16 72.0
Msa0585130 AT5G58890.1 40.506 79 40 2 1 72 1 79 1.32e-11 61.2
Msa0585130 AT5G55690.1 33.028 109 60 3 1 102 1 103 2.89e-11 60.1
Msa0585130 AT5G55690.2 33.028 109 60 3 1 102 1 103 2.89e-11 60.1

Find 42 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGCTTGTGCAGTAGTTTA+TGG 0.155587 4_2:+5646032 Msa0585130:CDS
CACAACAATGACTGGGAAAA+AGG 0.190222 4_2:+5645902 None:intergenic
TTCATTTAAGGTAGTAGATT+TGG 0.253282 4_2:-5646570 None:intergenic
TCATTGTCGATGATGATTCT+AGG 0.260008 4_2:+5645937 Msa0585130:CDS
GGATCTCTGGTTGAGGCTCA+TGG 0.322708 4_2:-5646058 None:intergenic
ATGTTGTCGCCATTATTCAT+AGG 0.374511 4_2:-5646462 None:intergenic
TGTGGTGATGGCCGGATCTC+TGG 0.406734 4_2:-5646071 None:intergenic
GGAAAGCAACATACAATAGA+AGG 0.409123 4_2:+5645958 Msa0585130:CDS
GATCTCTGGTTGAGGCTCAT+GGG 0.424537 4_2:-5646057 None:intergenic
TCAAAATTCAGAACAATGAC+TGG 0.434871 4_2:+5646113 Msa0585130:CDS
AGGATGGGTTCTGGCAATGC+TGG 0.443129 4_2:+5646435 Msa0585130:intron
TAAATGGCAAGGATGGGTTC+TGG 0.450639 4_2:+5646426 Msa0585130:intron
GGAGATGAGTCGGTGCCCTT+TGG 0.451552 4_2:+5646525 Msa0585130:CDS
ATGAGCCTCAACCAGAGATC+CGG 0.456813 4_2:+5646060 Msa0585130:CDS
AAGAGACTTACATGAAAGAA+AGG 0.463147 4_2:+5646165 Msa0585130:CDS
GCTGGAATGCCTATGAATAA+TGG 0.487571 4_2:+5646453 Msa0585130:CDS
AGAGATCCGGCCATCACCAC+AGG 0.494291 4_2:+5646073 Msa0585130:CDS
AGTAGATTTGGCAAAACTCT+AGG 0.518146 4_2:-5646558 None:intergenic
GAACTCCCTGTGGTGATGGC+CGG 0.518923 4_2:-5646079 None:intergenic
ATCACCACAGGGAGTTCAAA+CGG 0.524260 4_2:+5646085 Msa0585130:CDS
CTAGGATCGACCTCGCCAAA+GGG 0.529567 4_2:-5646540 None:intergenic
ATCTCTGGTTGAGGCTCATG+GGG 0.542152 4_2:-5646056 None:intergenic
AGAATCATCATCGACAATGA+AGG 0.542377 4_2:-5645934 None:intergenic
GTTTGAACTCCCTGTGGTGA+TGG 0.554673 4_2:-5646083 None:intergenic
ATTCAGAACAATGACTGGGC+GGG 0.563336 4_2:+5646118 Msa0585130:CDS
AACATACAATAGAAGGCGGA+AGG 0.566279 4_2:+5645965 Msa0585130:CDS
TCTAGGATCGACCTCGCCAA+AGG 0.568977 4_2:-5646541 None:intergenic
AGAGACTTACATGAAAGAAA+GGG 0.577279 4_2:+5646166 Msa0585130:CDS
TATGAACTAAGCACTTTGTG+TGG 0.579542 4_2:+5646005 Msa0585130:CDS
GATGGCCGGATCTCTGGTTG+AGG 0.581738 4_2:-5646065 None:intergenic
GCGGAAGGGTCTAATGAAGA+AGG 0.591410 4_2:+5645980 Msa0585130:CDS
CAAAATTCAGAACAATGACT+GGG 0.592691 4_2:+5646114 Msa0585130:CDS
GGCTTGATGATGAATAGTAA+TGG 0.609105 4_2:+5646504 Msa0585130:CDS
TGAGTCGGTGCCCTTTGGCG+AGG 0.619442 4_2:+5646530 Msa0585130:CDS
GCGGGAGAAGAGCAACAAAA+CGG 0.630975 4_2:+5646136 Msa0585130:CDS
GAATAGTAATGGAGATGAGT+CGG 0.650993 4_2:+5646515 Msa0585130:CDS
AAACTGAAGATACAAAGGCA+TGG 0.659981 4_2:+5646195 Msa0585130:CDS
ACATACAATAGAAGGCGGAA+GGG 0.677825 4_2:+5645966 Msa0585130:CDS
GAGATCCGGCCATCACCACA+GGG 0.683037 4_2:+5646074 Msa0585130:CDS
AATTCAGAACAATGACTGGG+CGG 0.687254 4_2:+5646117 Msa0585130:CDS
AAGCAACATACAATAGAAGG+CGG 0.694844 4_2:+5645961 Msa0585130:CDS
AGCACCGTTTGAACTCCCTG+TGG 0.787870 4_2:-5646089 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCAAAACATCTTTGT+TGG + chr4_2:5646298-5646317 Msa0585130:intron 20.0%
!! TATTCATTACTGAATGTAAA+TGG - chr4_2:5646408-5646427 None:intergenic 20.0%
!! TACATTCAGTAATGAATAAA+TGG + chr4_2:5646410-5646429 Msa0585130:intron 20.0%
!!! ATTGAAAGTCAATCTTTTAT+GGG + chr4_2:5646483-5646502 Msa0585130:CDS 20.0%
! TCTAAAAACTGAAGATACAA+AGG + chr4_2:5646190-5646209 Msa0585130:CDS 25.0%
! TATGCAGAATAATATGTCAT+TGG + chr4_2:5646275-5646294 Msa0585130:intron 25.0%
!! CATTGAAAGTCAATCTTTTA+TGG + chr4_2:5646482-5646501 Msa0585130:CDS 25.0%
TCAAAATTCAGAACAATGAC+TGG + chr4_2:5646113-5646132 Msa0585130:CDS 30.0%
CAAAATTCAGAACAATGACT+GGG + chr4_2:5646114-5646133 Msa0585130:CDS 30.0%
AAGAGACTTACATGAAAGAA+AGG + chr4_2:5646165-5646184 Msa0585130:CDS 30.0%
AGAGACTTACATGAAAGAAA+GGG + chr4_2:5646166-5646185 Msa0585130:CDS 30.0%
TCATTACTGAATGTAAATGG+TGG - chr4_2:5646405-5646424 None:intergenic 30.0%
TCAGTAATGAATAAATGGCA+AGG + chr4_2:5646415-5646434 Msa0585130:intron 30.0%
TAATGAATAAATGGCAAGGA+TGG + chr4_2:5646419-5646438 Msa0585130:intron 30.0%
AATGAATAAATGGCAAGGAT+GGG + chr4_2:5646420-5646439 Msa0585130:intron 30.0%
AGAATCATCATCGACAATGA+AGG - chr4_2:5645937-5645956 None:intergenic 35.0%
TCATTGTCGATGATGATTCT+AGG + chr4_2:5645937-5645956 Msa0585130:CDS 35.0%
GGAAAGCAACATACAATAGA+AGG + chr4_2:5645958-5645977 Msa0585130:CDS 35.0%
AAGCAACATACAATAGAAGG+CGG + chr4_2:5645961-5645980 Msa0585130:CDS 35.0%
TATGAACTAAGCACTTTGTG+TGG + chr4_2:5646005-5646024 Msa0585130:CDS 35.0%
AAACTGAAGATACAAAGGCA+TGG + chr4_2:5646195-5646214 Msa0585130:CDS 35.0%
! GATACAAAGGCATGGTAATA+AGG + chr4_2:5646203-5646222 Msa0585130:intron 35.0%
GTTGATTGACCACAAATTGA+AGG + chr4_2:5646320-5646339 Msa0585130:intron 35.0%
TTACTGAATGTAAATGGTGG+TGG - chr4_2:5646402-5646421 None:intergenic 35.0%
ATGTTGTCGCCATTATTCAT+AGG - chr4_2:5646465-5646484 None:intergenic 35.0%
!! GGCTTGATGATGAATAGTAA+TGG + chr4_2:5646504-5646523 Msa0585130:CDS 35.0%
! GAATAGTAATGGAGATGAGT+CGG + chr4_2:5646515-5646534 Msa0585130:CDS 35.0%
AGTAGATTTGGCAAAACTCT+AGG - chr4_2:5646561-5646580 None:intergenic 35.0%
AACATACAATAGAAGGCGGA+AGG + chr4_2:5645965-5645984 Msa0585130:CDS 40.0%
ACATACAATAGAAGGCGGAA+GGG + chr4_2:5645966-5645985 Msa0585130:CDS 40.0%
GAAGCTTGTGCAGTAGTTTA+TGG + chr4_2:5646032-5646051 Msa0585130:CDS 40.0%
AATTCAGAACAATGACTGGG+CGG + chr4_2:5646117-5646136 Msa0585130:CDS 40.0%
AAGGCATGGTAATAAGGAGA+AGG + chr4_2:5646209-5646228 Msa0585130:intron 40.0%
GATTGACCACAAATTGAAGG+AGG + chr4_2:5646323-5646342 Msa0585130:intron 40.0%
TACCAACCTCCTTCAATTTG+TGG - chr4_2:5646332-5646351 None:intergenic 40.0%
!! AATTGAAGGAGGTTGGTAGA+AGG + chr4_2:5646334-5646353 Msa0585130:intron 40.0%
TTGGAAGCAGAGGATAATAG+TGG + chr4_2:5646357-5646376 Msa0585130:intron 40.0%
!! ATGTAAATGGTGGTGGCATT+TGG - chr4_2:5646395-5646414 None:intergenic 40.0%
GCTGGAATGCCTATGAATAA+TGG + chr4_2:5646453-5646472 Msa0585130:CDS 40.0%
ATCACCACAGGGAGTTCAAA+CGG + chr4_2:5646085-5646104 Msa0585130:CDS 45.0%
ATTCAGAACAATGACTGGGC+GGG + chr4_2:5646118-5646137 Msa0585130:CDS 45.0%
GACCACAAATTGAAGGAGGT+TGG + chr4_2:5646327-5646346 Msa0585130:intron 45.0%
! TAAATGGCAAGGATGGGTTC+TGG + chr4_2:5646426-5646445 Msa0585130:intron 45.0%
GCGGAAGGGTCTAATGAAGA+AGG + chr4_2:5645980-5645999 Msa0585130:CDS 50.0%
ATCTCTGGTTGAGGCTCATG+GGG - chr4_2:5646059-5646078 None:intergenic 50.0%
GATCTCTGGTTGAGGCTCAT+GGG - chr4_2:5646060-5646079 None:intergenic 50.0%
ATGAGCCTCAACCAGAGATC+CGG + chr4_2:5646060-5646079 Msa0585130:CDS 50.0%
GTTTGAACTCCCTGTGGTGA+TGG - chr4_2:5646086-5646105 None:intergenic 50.0%
GCGGGAGAAGAGCAACAAAA+CGG + chr4_2:5646136-5646155 Msa0585130:CDS 50.0%
!! GAAGGAGGTTGGTAGAAGGT+TGG + chr4_2:5646338-5646357 Msa0585130:intron 50.0%
TGGTAGAAGGTTGGAAGCAG+AGG + chr4_2:5646347-5646366 Msa0585130:intron 50.0%
GGATCTCTGGTTGAGGCTCA+TGG - chr4_2:5646061-5646080 None:intergenic 55.0%
AGCACCGTTTGAACTCCCTG+TGG - chr4_2:5646092-5646111 None:intergenic 55.0%
! AGGATGGGTTCTGGCAATGC+TGG + chr4_2:5646435-5646454 Msa0585130:intron 55.0%
CTAGGATCGACCTCGCCAAA+GGG - chr4_2:5646543-5646562 None:intergenic 55.0%
! TCTAGGATCGACCTCGCCAA+AGG - chr4_2:5646544-5646563 None:intergenic 55.0%
GATGGCCGGATCTCTGGTTG+AGG - chr4_2:5646068-5646087 None:intergenic 60.0%
!! TGTGGTGATGGCCGGATCTC+TGG - chr4_2:5646074-5646093 None:intergenic 60.0%
AGAGATCCGGCCATCACCAC+AGG + chr4_2:5646073-5646092 Msa0585130:CDS 60.0%
GAGATCCGGCCATCACCACA+GGG + chr4_2:5646074-5646093 Msa0585130:CDS 60.0%
!! GAACTCCCTGTGGTGATGGC+CGG - chr4_2:5646082-5646101 None:intergenic 60.0%
! GGAGATGAGTCGGTGCCCTT+TGG + chr4_2:5646525-5646544 Msa0585130:CDS 60.0%
! TGAGTCGGTGCCCTTTGGCG+AGG + chr4_2:5646530-5646549 Msa0585130:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 5645909 5646587 5645909 ID=Msa0585130;Name=Msa0585130
chr4_2 mRNA 5645909 5646587 5645909 ID=Msa0585130-mRNA-1;Parent=Msa0585130;Name=Msa0585130-mRNA-1;_AED=0.19;_eAED=0.21;_QI=0|0|0|1|1|1|2|0|152
chr4_2 exon 5645909 5646216 5645909 ID=Msa0585130-mRNA-1:exon:1355;Parent=Msa0585130-mRNA-1
chr4_2 exon 5646437 5646587 5646437 ID=Msa0585130-mRNA-1:exon:1356;Parent=Msa0585130-mRNA-1
chr4_2 CDS 5645909 5646216 5645909 ID=Msa0585130-mRNA-1:cds;Parent=Msa0585130-mRNA-1
chr4_2 CDS 5646437 5646587 5646437 ID=Msa0585130-mRNA-1:cds;Parent=Msa0585130-mRNA-1
Gene Sequence

>Msa0585130

ATGACTGGGAAAAAGGTGAAACTTGCCTTCATTGTCGATGATGATTCTAGGAAAGCAACATACAATAGAAGGCGGAAGGGTCTAATGAAGAAGGTTTATGAACTAAGCACTTTGTGTGGAGTTGAAGCTTGTGCAGTAGTTTATGGACCCCATGAGCCTCAACCAGAGATCCGGCCATCACCACAGGGAGTTCAAACGGTGCTCTCAAAATTCAGAACAATGACTGGGCGGGAGAAGAGCAACAAAACGGTTGATCAAGAGACTTACATGAAAGAAAGGGTTCTAAAAACTGAAGATACAAAGGCATGGATGGGTTCTGGCAATGCTGGAATGCCTATGAATAATGGCGACAACATTGAAAGTCAATCTTTTATGGGCTTGATGATGAATAGTAATGGAGATGAGTCGGTGCCCTTTGGCGAGGTCGATCCTAGAGTTTTGCCAAATCTACTACCTTAA

Protein sequence

>Msa0585130

MTGKKVKLAFIVDDDSRKATYNRRRKGLMKKVYELSTLCGVEACAVVYGPHEPQPEIRPSPQGVQTVLSKFRTMTGREKSNKTVDQETYMKERVLKTEDTKAWMGSGNAGMPMNNGDNIESQSFMGLMMNSNGDESVPFGEVDPRVLPNLLP*