AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0591820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0591820 MtrunA17_Chr4g0009851 87.500 312 38 1 1 311 1 312 0.0 565
Msa0591820 MtrunA17_Chr4g0009981 84.556 259 40 0 53 311 1 259 2.14e-167 465
Msa0591820 MtrunA17_Chr4g0009901 71.250 240 69 0 1 240 1 240 1.90e-120 347
Msa0591820 MtrunA17_Chr4g0011071 77.372 137 31 0 55 191 35 171 7.12e-75 227
Msa0591820 MtrunA17_Chr6g0471041 46.711 304 141 8 1 294 1 293 2.03e-73 232
Msa0591820 MtrunA17_Chr1g0161431 36.667 360 163 8 13 307 14 373 7.43e-66 217
Msa0591820 MtrunA17_Chr4g0009991 76.923 117 26 1 101 217 1 116 1.15e-57 181
Msa0591820 MtrunA17_Chr3g0123131 40.254 236 113 3 13 220 16 251 1.95e-57 194
Msa0591820 MtrunA17_Chr4g0067981 40.598 234 113 2 13 220 34 267 5.91e-56 192
Msa0591820 MtrunA17_Chr8g0376881 39.744 234 115 3 13 220 28 261 1.36e-55 188
Msa0591820 MtrunA17_Chr2g0294911 41.379 232 112 2 13 220 32 263 1.88e-55 189
Msa0591820 MtrunA17_Chr7g0275401 37.295 244 124 3 13 227 8 251 3.45e-51 171
Msa0591820 MtrunA17_Chr1g0149841 39.407 236 115 3 13 220 8 243 1.97e-49 171
Msa0591820 MtrunA17_Chr8g0391911 40.000 230 112 3 14 217 13 242 2.28e-48 162
Msa0591820 MtrunA17_Chr8g0383961 42.481 266 115 8 1 254 1 240 3.91e-48 162
Msa0591820 MtrunA17_Chr3g0137101 38.333 240 116 3 13 220 11 250 1.71e-47 168
Msa0591820 MtrunA17_Chr7g0275891 33.670 297 152 7 13 266 8 302 1.14e-44 155
Msa0591820 MtrunA17_Chr5g0423031 71.910 89 25 0 1 89 1 89 6.34e-43 145
Msa0591820 MtrunA17_Chr1g0149851 33.113 302 158 6 13 270 8 309 6.92e-43 154
Msa0591820 MtrunA17_Chr4g0074401 32.794 247 138 4 13 231 20 266 1.84e-42 154
Msa0591820 MtrunA17_Chr4g0010001 71.910 89 25 0 1 89 1 89 1.94e-42 141
Msa0591820 MtrunA17_Chr4g0074411 34.016 244 136 4 13 231 21 264 3.24e-42 154
Msa0591820 MtrunA17_Chr2g0304841 34.127 252 129 4 13 227 21 272 1.76e-41 152
Msa0591820 MtrunA17_Chr3g0134461 39.130 207 99 4 13 217 11 192 3.04e-40 139
Msa0591820 MtrunA17_Chr3g0134481 34.389 221 107 3 4 223 4 187 4.39e-38 133
Msa0591820 MtrunA17_Chr6g0456631 43.243 148 82 1 14 159 13 160 2.18e-37 131
Msa0591820 MtrunA17_Chr4g0074421 35.912 181 94 3 59 217 2 182 2.40e-36 129
Msa0591820 MtrunA17_Chr1g0149831 37.778 180 82 3 68 220 1 177 3.40e-30 114
Msa0591820 MtrunA17_Chr6g0473731 31.319 182 107 3 89 257 10 186 2.03e-22 97.1
Msa0591820 MtrunA17_Chr7g0223971 28.169 213 97 4 91 254 1 206 1.51e-17 82.8
Msa0591820 MtrunA17_Chr5g0417521 47.826 69 31 2 168 231 15 83 1.03e-13 70.9
Msa0591820 MtrunA17_Chr2g0324891 37.363 91 55 1 136 224 127 217 1.30e-13 70.9
Msa0591820 MtrunA17_Chr7g0261911 43.011 93 48 3 159 249 253 342 1.76e-13 70.9
Msa0591820 MtrunA17_Chr7g0254981 41.758 91 50 2 166 255 48 136 2.23e-13 69.3
Msa0591820 MtrunA17_Chr6g0461641 55.556 54 23 1 168 220 42 95 5.45e-13 68.9
Msa0591820 MtrunA17_Chr4g0040481 46.552 58 31 0 167 224 157 214 6.45e-13 68.9
Msa0591820 MtrunA17_Chr5g0400241 34.940 83 54 0 167 249 310 392 1.26e-12 68.2
Msa0591820 MtrunA17_Chr4g0042451 51.852 54 25 1 168 220 17 70 1.36e-12 67.4
Msa0591820 MtrunA17_Chr2g0321461 53.704 54 24 1 168 220 45 98 2.16e-12 67.0
Msa0591820 MtrunA17_Chr2g0291021 57.407 54 22 1 168 220 49 102 6.03e-12 65.5
Msa0591820 MtrunA17_Chr8g0345901 28.689 122 83 2 14 131 36 157 7.33e-12 65.9
Msa0591820 MtrunA17_Chr7g0274471 47.222 72 36 2 168 237 218 289 1.01e-11 65.5
Msa0591820 MtrunA17_Chr4g0054981 50.000 54 27 0 167 220 309 362 1.05e-11 65.5
Msa0591820 MtrunA17_Chr7g0242521 51.429 70 25 3 155 220 5 69 2.44e-11 62.8
Msa0591820 MtrunA17_Chr7g0255711 50.000 54 27 0 167 220 90 143 2.50e-11 63.5
Msa0591820 MtrunA17_Chr1g0198601 46.575 73 31 2 149 220 199 264 3.04e-11 63.5
Msa0591820 MtrunA17_Chr3g0127941 29.600 125 78 3 14 131 56 177 3.14e-11 63.9
Msa0591820 MtrunA17_Chr1g0190301 50.000 64 30 2 159 220 165 228 3.31e-11 63.5
Msa0591820 MtrunA17_Chr4g0031961 43.478 69 38 1 156 224 120 187 3.74e-11 63.2
Msa0591820 MtrunA17_Chr7g0258551 49.123 57 28 1 165 220 209 265 4.03e-11 63.2
Msa0591820 MtrunA17_Chr1g0175091 44.118 68 35 2 154 220 246 311 5.63e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0591820 AT2G01760.1 38.431 255 118 5 13 230 10 262 5.45e-55 183
Msa0591820 AT1G67710.1 41.350 237 110 4 13 220 10 246 1.61e-53 183
Msa0591820 AT3G16857.1 38.583 254 110 6 13 220 36 289 6.65e-50 176
Msa0591820 AT3G16857.2 38.583 254 110 6 13 220 36 289 7.33e-50 176
Msa0591820 AT2G01760.2 34.629 283 118 6 13 230 10 290 5.71e-49 168
Msa0591820 AT4G31920.1 35.772 246 143 4 13 246 16 258 7.62e-47 166
Msa0591820 AT2G25180.1 37.768 233 119 5 13 220 16 247 1.17e-43 157
Msa0591820 AT5G58080.1 37.768 233 117 5 13 220 17 246 6.44e-40 148
Msa0591820 AT3G16857.3 37.156 218 91 6 49 220 2 219 1.89e-38 144
Msa0591820 AT2G01760.3 36.181 199 89 4 68 230 1 197 4.59e-37 135
Msa0591820 AT1G67710.2 40.884 181 79 3 68 220 1 181 3.97e-34 130
Msa0591820 AT5G49240.1 30.508 236 137 4 9 217 37 272 3.06e-30 116
Msa0591820 AT1G49190.1 26.074 326 194 9 16 303 36 352 1.73e-27 110
Msa0591820 AT3G62670.1 28.689 244 149 6 6 226 33 274 9.35e-27 109
Msa0591820 AT1G67710.3 39.752 161 69 3 88 220 12 172 4.75e-26 107
Msa0591820 AT5G07210.2 27.586 261 133 7 14 220 16 274 1.45e-24 104
Msa0591820 AT5G07210.1 26.766 269 133 8 14 220 16 282 4.72e-22 97.1
Msa0591820 AT1G68210.1 24.013 304 170 7 8 255 7 305 6.19e-19 87.8
Msa0591820 AT2G27070.1 25.758 264 137 9 14 220 16 277 6.49e-19 87.4
Msa0591820 AT5G29000.1 34.161 161 90 5 103 254 123 276 1.81e-17 82.4
Msa0591820 AT5G29000.3 34.161 161 90 5 103 254 123 276 1.81e-17 82.4
Msa0591820 AT5G29000.2 34.161 161 90 5 103 254 166 319 1.87e-17 82.8
Msa0591820 AT5G29000.4 36.429 140 73 5 103 233 166 298 2.09e-17 81.6
Msa0591820 AT1G49190.2 31.933 119 80 1 16 133 36 154 3.47e-15 76.6
Msa0591820 AT5G44190.1 50.000 60 30 0 165 224 144 203 9.57e-14 71.6
Msa0591820 AT5G44190.2 50.000 60 30 0 165 224 144 203 1.13e-13 71.2
Msa0591820 AT3G04030.1 55.556 54 23 1 168 220 45 98 4.11e-13 69.7
Msa0591820 AT3G04030.3 55.556 54 23 1 168 220 45 98 4.60e-13 69.7
Msa0591820 AT4G28610.1 46.067 89 44 3 168 254 225 311 6.31e-13 69.3
Msa0591820 AT2G01060.1 50.769 65 31 1 157 220 4 68 8.72e-13 67.8
Msa0591820 AT5G18240.2 55.556 54 23 1 168 220 45 98 8.94e-13 68.6
Msa0591820 AT5G18240.3 55.556 54 23 1 168 220 45 98 8.94e-13 68.6
Msa0591820 AT5G18240.5 55.556 54 23 1 168 220 45 98 1.02e-12 68.6
Msa0591820 AT3G04450.3 50.000 58 28 1 164 220 234 291 1.04e-12 68.2
Msa0591820 AT3G04450.1 42.500 80 39 2 164 242 234 307 1.05e-12 68.6
Msa0591820 AT5G18240.1 55.556 54 23 1 168 220 45 98 1.11e-12 68.6
Msa0591820 AT5G18240.4 55.556 54 23 1 168 220 45 98 1.11e-12 68.6
Msa0591820 AT3G04450.4 42.500 80 39 2 164 242 194 267 1.46e-12 68.2
Msa0591820 AT3G04450.2 42.500 80 39 2 164 242 194 267 1.46e-12 68.2
Msa0591820 AT5G60100.2 30.894 123 79 3 14 131 64 185 1.56e-12 68.2
Msa0591820 AT5G60100.7 30.894 123 79 3 14 131 64 185 1.79e-12 67.8
Msa0591820 AT5G60100.6 30.894 123 79 3 14 131 64 185 1.79e-12 67.8
Msa0591820 AT5G60100.4 30.894 123 79 3 14 131 64 185 1.79e-12 67.8
Msa0591820 AT5G60100.5 30.894 123 79 3 14 131 64 185 2.02e-12 67.8
Msa0591820 AT5G60100.3 30.894 123 79 3 14 131 64 185 2.02e-12 67.8
Msa0591820 AT5G60100.1 30.894 123 79 3 14 131 64 185 2.02e-12 67.8
Msa0591820 AT5G59570.2 43.056 72 38 1 149 220 125 193 2.40e-12 66.6
Msa0591820 AT5G59570.1 43.056 72 38 1 149 220 125 193 2.40e-12 66.6
Msa0591820 AT3G04030.2 52.830 53 25 0 168 220 45 97 2.68e-12 67.4
Msa0591820 AT5G24470.1 28.571 126 80 3 13 131 49 171 3.73e-12 67.4
Msa0591820 AT2G20570.1 48.276 58 30 0 167 224 152 209 4.02e-12 67.0
Msa0591820 AT3G46640.2 44.156 77 40 2 144 220 123 196 7.64e-12 65.5
Msa0591820 AT3G46640.1 44.156 77 40 2 144 220 123 196 7.64e-12 65.5
Msa0591820 AT3G46640.3 44.156 77 40 2 144 220 123 196 8.36e-12 65.5
Msa0591820 AT1G69580.1 51.852 54 25 1 168 220 34 87 9.01e-12 65.5
Msa0591820 AT2G40970.1 50.000 54 27 0 167 220 104 157 9.05e-12 64.7
Msa0591820 AT5G05090.1 43.529 85 36 3 136 220 61 133 9.39e-12 64.7
Msa0591820 AT1G69580.2 51.852 54 25 1 168 220 34 87 9.75e-12 65.5
Msa0591820 AT4G18020.8 38.158 76 44 1 150 225 281 353 1.53e-11 65.1
Msa0591820 AT4G18020.11 38.158 76 44 1 150 225 281 353 1.53e-11 65.1
Msa0591820 AT4G18020.4 38.158 76 44 1 150 225 281 353 1.53e-11 65.1
Msa0591820 AT4G18020.5 38.158 76 44 1 150 225 281 353 1.53e-11 65.1
Msa0591820 AT4G18020.10 38.158 76 44 1 150 225 281 353 1.53e-11 65.1
Msa0591820 AT4G18020.7 38.158 76 44 1 150 225 281 353 1.53e-11 65.1
Msa0591820 AT4G18020.3 38.158 76 44 1 150 225 281 353 1.67e-11 65.1
Msa0591820 AT4G18020.2 38.158 76 44 1 150 225 281 353 1.67e-11 65.1
Msa0591820 AT4G18020.1 38.158 76 44 1 150 225 281 353 1.67e-11 65.1
Msa0591820 AT4G18020.6 38.158 76 44 1 150 225 281 353 1.67e-11 65.1
Msa0591820 AT4G18020.9 38.158 76 44 1 150 225 281 353 1.67e-11 65.1
Msa0591820 AT3G10760.1 50.000 54 27 0 167 220 104 157 1.94e-11 64.3
Msa0591820 AT2G20570.2 49.091 55 28 0 170 224 171 225 2.92e-11 64.3
Msa0591820 AT4G04605.1 37.624 101 51 3 166 264 12 102 4.62e-11 62.0
Msa0591820 AT5G06800.3 53.704 54 24 1 168 220 192 245 9.50e-11 62.0

Find 54 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGTTGCCAGCCATTTAC+AGG 0.283862 4_2:-16703890 Msa0591820:intron
GTGTAATCGATGCAATTATA+AGG 0.312923 4_2:-16704712 Msa0591820:CDS
ACATCTCCTCGACGAAATTA+AGG 0.321564 4_2:+16704733 None:intergenic
CAACAACATTGGGTGGAAAT+TGG 0.345706 4_2:-16703229 Msa0591820:CDS
GATAAATCATCATCAACATA+TGG 0.380393 4_2:+16703157 None:intergenic
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4_2:-16704296 Msa0591820:CDS
CGAATATTGGACTAAGCCTT+TGG 0.392176 4_2:-16704283 Msa0591820:CDS
ATCAGAAGCCATGCTGCATT+TGG 0.416557 4_2:+16704581 None:intergenic
CAAAGAAGGCCCGTTTGTCT+TGG 0.418636 4_2:-16704113 Msa0591820:CDS
GTTTCAAGAAAATAGAAAAC+GGG 0.418887 4_2:-16704172 Msa0591820:CDS
CTTTCTTTCCAACATGTTGA+AGG 0.419409 4_2:+16704557 None:intergenic
TGGCAACATGTTATAAGAAA+AGG 0.422808 4_2:-16704237 Msa0591820:CDS
TGATGGTTTGTCGGTGAAAA+GGG 0.427097 4_2:+16704803 None:intergenic
AAAAGTGTTGCTCATAATGT+TGG 0.434928 4_2:+16703201 None:intergenic
AAAAGAAACACTTGCAAAGA+AGG 0.435703 4_2:-16704127 Msa0591820:CDS
CAGAATTATGAAGAAAACTT+TGG 0.439068 4_2:+16703281 None:intergenic
TCAACATATGGGAACAAGTT+GGG 0.453000 4_2:+16703169 None:intergenic
ATGATGGTTTGTCGGTGAAA+AGG 0.455733 4_2:+16704802 None:intergenic
CTTCAACATGTTGGAAAGAA+AGG 0.459586 4_2:-16704556 Msa0591820:CDS
AATAAATTCTTACTAATGAC+AGG 0.461511 4_2:+16704444 None:intergenic
TTCTCGCGACCAAGACAAAC+GGG 0.470376 4_2:+16704104 None:intergenic
TGTAATCGATGCAATTATAA+GGG 0.476930 4_2:-16704711 Msa0591820:intron
TTGCGATGCACAACAACATT+GGG 0.477561 4_2:-16703239 Msa0591820:CDS
TTGAGACCTTCTGATAATGA+TGG 0.479264 4_2:+16704786 None:intergenic
TACTCACTCAATACCGGAGC+AGG 0.481604 4_2:-16703305 Msa0591820:CDS
TTCTGATAATGATGGTTTGT+CGG 0.493949 4_2:+16704794 None:intergenic
AAGTCGTAAACATCCATGTT+TGG 0.494866 4_2:+16704498 None:intergenic
ATATGAAATTGTTGGAAGCT+CGG 0.504899 4_2:-16704196 Msa0591820:CDS
TGTTTCAAGAAAATAGAAAA+CGG 0.506234 4_2:-16704173 Msa0591820:CDS
AGGAAAGAATCATACTATCA+AGG 0.510494 4_2:+16704043 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4_2:-16704312 Msa0591820:CDS
AAGCTAAATTGTGTTTCAAG+AGG 0.513615 4_2:+16703334 None:intergenic
AGAAAACTTTGGTCCTGCTC+CGG 0.527471 4_2:+16703292 None:intergenic
TTCTGATGCCTTCAACATGT+TGG 0.527887 4_2:-16704565 Msa0591820:CDS
TTATGAAGTCTATTGAATGT+GGG 0.532174 4_2:-16704311 Msa0591820:CDS
AAAACGAACCTGTAAATGGC+TGG 0.538065 4_2:+16703882 None:intergenic
AGCTCAACCGATGAGACAAA+AGG 0.540383 4_2:-16703382 Msa0591820:CDS
ATTCTCGCGACCAAGACAAA+CGG 0.543227 4_2:+16704103 None:intergenic
CTACGTGCAGTCAATCAACT+AGG 0.547867 4_2:-16704066 Msa0591820:CDS
ATTGCGATGCACAACAACAT+TGG 0.547991 4_2:-16703240 Msa0591820:CDS
ATAAATCATCATCAACATAT+GGG 0.548211 4_2:+16703158 None:intergenic
ATCAACATATGGGAACAAGT+TGG 0.552596 4_2:+16703168 None:intergenic
AGAAGCTACTCACTCAATAC+CGG 0.567403 4_2:-16703311 Msa0591820:CDS
ATCATTATCAGAAGGTCTCA+AGG 0.582734 4_2:-16704784 Msa0591820:CDS
TTCAGTTACCAAATGCAGCA+TGG 0.588519 4_2:-16704589 Msa0591820:intron
CGATGCACAACAACATTGGG+TGG 0.592869 4_2:-16703236 Msa0591820:CDS
AGAACTTTCTGCAGAATCAA+CGG 0.593830 4_2:+16704335 None:intergenic
TCTTATAACATGTTGCCACA+TGG 0.594344 4_2:+16704242 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.598969 4_2:-16704310 Msa0591820:CDS
TGATGCTCATATGCCAAACA+TGG 0.604750 4_2:-16704511 Msa0591820:CDS
CACTCTCCTTAATTTCGTCG+AGG 0.625060 4_2:-16704739 Msa0591820:CDS
GACAAACCATCATTATCAGA+AGG 0.636966 4_2:-16704792 Msa0591820:CDS
ATGATTTATCATCACTTGTG+TGG 0.696325 4_2:-16703144 Msa0591820:CDS
TTTCAAGAAAATAGAAAACG+GGG 0.722642 4_2:-16704171 Msa0591820:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAAAATATGAAATTGT+TGG - chr4_2:16703744-16703763 Msa0591820:intron 15.0%
!! AACATAGTAAAAAAGTAATT+AGG + chr4_2:16703928-16703947 None:intergenic 15.0%
!! AACAAAAAATATGAAATTGT+TGG - chr4_2:16703744-16703763 Msa0591820:intron 15.0%
!! AACATAGTAAAAAAGTAATT+AGG + chr4_2:16703928-16703947 None:intergenic 15.0%
!! AAAGATGAAAACTTTGAATA+AGG + chr4_2:16703280-16703299 None:intergenic 20.0%
!!! TTCAAAGTTTTCATCTTTTT+TGG - chr4_2:16703282-16703301 Msa0591820:CDS 20.0%
!! AATAAATTCTTACTAATGAC+AGG + chr4_2:16703507-16703526 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG - chr4_2:16703590-16703609 Msa0591820:intron 20.0%
!! TGTTTCAAGAAAATAGAAAA+CGG - chr4_2:16703775-16703794 Msa0591820:intron 20.0%
!! TGAAAAATAAGAGAAAAACA+TGG + chr4_2:16703982-16704001 None:intergenic 20.0%
!!! ATGTTTTTCTCTTATTTTTC+AGG - chr4_2:16703981-16704000 Msa0591820:intron 20.0%
!!! TGTTTTTCTCTTATTTTTCA+GGG - chr4_2:16703982-16704001 Msa0591820:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG + chr4_2:16704014-16704033 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG - chr4_2:16704013-16704032 Msa0591820:intron 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG + chr4_2:16704045-16704064 None:intergenic 20.0%
!! TGATTGTAAACTGAAATTTA+TGG - chr4_2:16704477-16704496 Msa0591820:CDS 20.0%
!! ATAAATCATCATCAACATAT+GGG + chr4_2:16704793-16704812 None:intergenic 20.0%
!! AAAGATGAAAACTTTGAATA+AGG + chr4_2:16703280-16703299 None:intergenic 20.0%
!!! TTCAAAGTTTTCATCTTTTT+TGG - chr4_2:16703282-16703301 Msa0591820:CDS 20.0%
!! AATAAATTCTTACTAATGAC+AGG + chr4_2:16703507-16703526 None:intergenic 20.0%
!!! TTATTTCTTTTTTGTAGTGA+TGG - chr4_2:16703590-16703609 Msa0591820:intron 20.0%
!! TGTTTCAAGAAAATAGAAAA+CGG - chr4_2:16703775-16703794 Msa0591820:intron 20.0%
!! TGAAAAATAAGAGAAAAACA+TGG + chr4_2:16703982-16704001 None:intergenic 20.0%
!!! ATGTTTTTCTCTTATTTTTC+AGG - chr4_2:16703981-16704000 Msa0591820:intron 20.0%
!!! TGTTTTTCTCTTATTTTTCA+GGG - chr4_2:16703982-16704001 Msa0591820:intron 20.0%
!!! ATGATTTTCAGAATCTTTTT+TGG + chr4_2:16704014-16704033 None:intergenic 20.0%
!!! AAAAAAGATTCTGAAAATCA+TGG - chr4_2:16704013-16704032 Msa0591820:intron 20.0%
!!! AACATGTTCTTTTTTCATAT+CGG + chr4_2:16704045-16704064 None:intergenic 20.0%
!! TGATTGTAAACTGAAATTTA+TGG - chr4_2:16704477-16704496 Msa0591820:CDS 20.0%
!! ATAAATCATCATCAACATAT+GGG + chr4_2:16704793-16704812 None:intergenic 20.0%
! TGTAATCGATGCAATTATAA+GGG - chr4_2:16703237-16703256 Msa0591820:CDS 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4_2:16703636-16703655 Msa0591820:intron 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4_2:16703637-16703656 Msa0591820:intron 25.0%
! GTTTCAAGAAAATAGAAAAC+GGG - chr4_2:16703776-16703795 Msa0591820:intron 25.0%
! TTTCAAGAAAATAGAAAACG+GGG - chr4_2:16703777-16703796 Msa0591820:intron 25.0%
!!! GTTTTTCTCTTATTTTTCAG+GGG - chr4_2:16703983-16704002 Msa0591820:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG + chr4_2:16704044-16704063 None:intergenic 25.0%
! ATGCATAAGCTATTAAATTC+AGG + chr4_2:16704298-16704317 None:intergenic 25.0%
! TGAATTTAATAGCTTATGCA+TGG - chr4_2:16704297-16704316 Msa0591820:CDS 25.0%
! TAACAGCTAGAAAAAGAATT+TGG + chr4_2:16704332-16704351 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG + chr4_2:16704441-16704460 None:intergenic 25.0%
! CAGAATTATGAAGAAAACTT+TGG + chr4_2:16704670-16704689 None:intergenic 25.0%
! GATAAATCATCATCAACATA+TGG + chr4_2:16704794-16704813 None:intergenic 25.0%
! TGTAATCGATGCAATTATAA+GGG - chr4_2:16703237-16703256 Msa0591820:CDS 25.0%
! ATTATGAAGTCTATTGAATG+TGG - chr4_2:16703636-16703655 Msa0591820:intron 25.0%
! TTATGAAGTCTATTGAATGT+GGG - chr4_2:16703637-16703656 Msa0591820:intron 25.0%
! GTTTCAAGAAAATAGAAAAC+GGG - chr4_2:16703776-16703795 Msa0591820:intron 25.0%
! TTTCAAGAAAATAGAAAACG+GGG - chr4_2:16703777-16703796 Msa0591820:intron 25.0%
!!! GTTTTTCTCTTATTTTTCAG+GGG - chr4_2:16703983-16704002 Msa0591820:intron 25.0%
!!! ACATGTTCTTTTTTCATATC+GGG + chr4_2:16704044-16704063 None:intergenic 25.0%
! ATGCATAAGCTATTAAATTC+AGG + chr4_2:16704298-16704317 None:intergenic 25.0%
! TGAATTTAATAGCTTATGCA+TGG - chr4_2:16704297-16704316 Msa0591820:CDS 25.0%
! TAACAGCTAGAAAAAGAATT+TGG + chr4_2:16704332-16704351 None:intergenic 25.0%
! AGTAGTATATTATCAACAAG+AGG + chr4_2:16704441-16704460 None:intergenic 25.0%
! CAGAATTATGAAGAAAACTT+TGG + chr4_2:16704670-16704689 None:intergenic 25.0%
! GATAAATCATCATCAACATA+TGG + chr4_2:16704794-16704813 None:intergenic 25.0%
! TTCTGATAATGATGGTTTGT+CGG + chr4_2:16703157-16703176 None:intergenic 30.0%
GTGTAATCGATGCAATTATA+AGG - chr4_2:16703236-16703255 Msa0591820:CDS 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4_2:16703638-16703657 Msa0591820:intron 30.0%
!! TTGAACTGTTTTTCAACCAA+AGG + chr4_2:16703684-16703703 None:intergenic 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4_2:16703711-16703730 Msa0591820:intron 30.0%
ATATGAAATTGTTGGAAGCT+CGG - chr4_2:16703752-16703771 Msa0591820:intron 30.0%
AAAAGAAACACTTGCAAAGA+AGG - chr4_2:16703821-16703840 Msa0591820:intron 30.0%
AGGAAAGAATCATACTATCA+AGG + chr4_2:16703908-16703927 None:intergenic 30.0%
TACACAATTACAACAGACTT+AGG + chr4_2:16704170-16704189 None:intergenic 30.0%
TTGTATAACATGCAAGACTA+TGG + chr4_2:16704213-16704232 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+CGG + chr4_2:16704423-16704442 None:intergenic 30.0%
AAGCTAAATTGTGTTTCAAG+AGG + chr4_2:16704617-16704636 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG + chr4_2:16704750-16704769 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG - chr4_2:16704804-16704823 Msa0591820:CDS 30.0%
! TTCTGATAATGATGGTTTGT+CGG + chr4_2:16703157-16703176 None:intergenic 30.0%
GTGTAATCGATGCAATTATA+AGG - chr4_2:16703236-16703255 Msa0591820:CDS 30.0%
TATGAAGTCTATTGAATGTG+GGG - chr4_2:16703638-16703657 Msa0591820:intron 30.0%
!! TTGAACTGTTTTTCAACCAA+AGG + chr4_2:16703684-16703703 None:intergenic 30.0%
TGGCAACATGTTATAAGAAA+AGG - chr4_2:16703711-16703730 Msa0591820:intron 30.0%
ATATGAAATTGTTGGAAGCT+CGG - chr4_2:16703752-16703771 Msa0591820:intron 30.0%
AAAAGAAACACTTGCAAAGA+AGG - chr4_2:16703821-16703840 Msa0591820:intron 30.0%
AGGAAAGAATCATACTATCA+AGG + chr4_2:16703908-16703927 None:intergenic 30.0%
TACACAATTACAACAGACTT+AGG + chr4_2:16704170-16704189 None:intergenic 30.0%
TTGTATAACATGCAAGACTA+TGG + chr4_2:16704213-16704232 None:intergenic 30.0%
AGAGGATATATAGAAGCTAA+CGG + chr4_2:16704423-16704442 None:intergenic 30.0%
AAGCTAAATTGTGTTTCAAG+AGG + chr4_2:16704617-16704636 None:intergenic 30.0%
!! AAAAGTGTTGCTCATAATGT+TGG + chr4_2:16704750-16704769 None:intergenic 30.0%
ATGATTTATCATCACTTGTG+TGG - chr4_2:16704804-16704823 Msa0591820:CDS 30.0%
GACAAACCATCATTATCAGA+AGG - chr4_2:16703156-16703175 Msa0591820:CDS 35.0%
! TTGAGACCTTCTGATAATGA+TGG + chr4_2:16703165-16703184 None:intergenic 35.0%
ATCATTATCAGAAGGTCTCA+AGG - chr4_2:16703164-16703183 Msa0591820:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG + chr4_2:16703394-16703413 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG - chr4_2:16703392-16703411 Msa0591820:CDS 35.0%
AAGTCGTAAACATCCATGTT+TGG + chr4_2:16703453-16703472 None:intergenic 35.0%
!!! GACAGGAATTTTGAGTTGTA+GGG + chr4_2:16703490-16703509 None:intergenic 35.0%
!!! TGACAGGAATTTTGAGTTGT+AGG + chr4_2:16703491-16703510 None:intergenic 35.0%
AGAACTTTCTGCAGAATCAA+CGG + chr4_2:16703616-16703635 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG - chr4_2:16703691-16703710 Msa0591820:intron 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4_2:16703709-16703728 None:intergenic 35.0%
! CTTTGCAAGTGTTTCTTTTG+AGG + chr4_2:16703820-16703839 None:intergenic 35.0%
TGCAAAAACGAACCTGTAAA+TGG + chr4_2:16704073-16704092 None:intergenic 35.0%
AAAAGATCACTGACAAACCT+AGG + chr4_2:16704146-16704165 None:intergenic 35.0%
! CATCTTACCTTTTGTCTCAT+CGG + chr4_2:16704576-16704595 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG + chr4_2:16704782-16704801 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG + chr4_2:16704783-16704802 None:intergenic 35.0%
GACAAACCATCATTATCAGA+AGG - chr4_2:16703156-16703175 Msa0591820:CDS 35.0%
! TTGAGACCTTCTGATAATGA+TGG + chr4_2:16703165-16703184 None:intergenic 35.0%
ATCATTATCAGAAGGTCTCA+AGG - chr4_2:16703164-16703183 Msa0591820:CDS 35.0%
CTTTCTTTCCAACATGTTGA+AGG + chr4_2:16703394-16703413 None:intergenic 35.0%
CTTCAACATGTTGGAAAGAA+AGG - chr4_2:16703392-16703411 Msa0591820:CDS 35.0%
AAGTCGTAAACATCCATGTT+TGG + chr4_2:16703453-16703472 None:intergenic 35.0%
!!! GACAGGAATTTTGAGTTGTA+GGG + chr4_2:16703490-16703509 None:intergenic 35.0%
!!! TGACAGGAATTTTGAGTTGT+AGG + chr4_2:16703491-16703510 None:intergenic 35.0%
AGAACTTTCTGCAGAATCAA+CGG + chr4_2:16703616-16703635 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG - chr4_2:16703691-16703710 Msa0591820:intron 35.0%
TCTTATAACATGTTGCCACA+TGG + chr4_2:16703709-16703728 None:intergenic 35.0%
! CTTTGCAAGTGTTTCTTTTG+AGG + chr4_2:16703820-16703839 None:intergenic 35.0%
TGCAAAAACGAACCTGTAAA+TGG + chr4_2:16704073-16704092 None:intergenic 35.0%
AAAAGATCACTGACAAACCT+AGG + chr4_2:16704146-16704165 None:intergenic 35.0%
! CATCTTACCTTTTGTCTCAT+CGG + chr4_2:16704576-16704595 None:intergenic 35.0%
TCAACATATGGGAACAAGTT+GGG + chr4_2:16704782-16704801 None:intergenic 35.0%
ATCAACATATGGGAACAAGT+TGG + chr4_2:16704783-16704802 None:intergenic 35.0%
! TGATGGTTTGTCGGTGAAAA+GGG + chr4_2:16703148-16703167 None:intergenic 40.0%
! ATGATGGTTTGTCGGTGAAA+AGG + chr4_2:16703149-16703168 None:intergenic 40.0%
ACATCTCCTCGACGAAATTA+AGG + chr4_2:16703218-16703237 None:intergenic 40.0%
TTCAGTTACCAAATGCAGCA+TGG - chr4_2:16703359-16703378 Msa0591820:CDS 40.0%
TTCTGATGCCTTCAACATGT+TGG - chr4_2:16703383-16703402 Msa0591820:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG - chr4_2:16703437-16703456 Msa0591820:intron 40.0%
CGAATATTGGACTAAGCCTT+TGG - chr4_2:16703665-16703684 Msa0591820:intron 40.0%
AAAACGAACCTGTAAATGGC+TGG + chr4_2:16704069-16704088 None:intergenic 40.0%
AAACCTAGGCAACTAGACTT+AGG + chr4_2:16704132-16704151 None:intergenic 40.0%
GAGTAGCTTCAGCTACTTAT+AGG - chr4_2:16704253-16704272 Msa0591820:CDS 40.0%
CAGGTTCGTTTCATTTACAC+CGG + chr4_2:16704279-16704298 None:intergenic 40.0%
AGAAGCTACTCACTCAATAC+CGG - chr4_2:16704637-16704656 Msa0591820:intron 40.0%
ATTGCGATGCACAACAACAT+TGG - chr4_2:16704708-16704727 Msa0591820:intron 40.0%
TTGCGATGCACAACAACATT+GGG - chr4_2:16704709-16704728 Msa0591820:intron 40.0%
CAACAACATTGGGTGGAAAT+TGG - chr4_2:16704719-16704738 Msa0591820:CDS 40.0%
! TGATGGTTTGTCGGTGAAAA+GGG + chr4_2:16703148-16703167 None:intergenic 40.0%
! ATGATGGTTTGTCGGTGAAA+AGG + chr4_2:16703149-16703168 None:intergenic 40.0%
ACATCTCCTCGACGAAATTA+AGG + chr4_2:16703218-16703237 None:intergenic 40.0%
TTCAGTTACCAAATGCAGCA+TGG - chr4_2:16703359-16703378 Msa0591820:CDS 40.0%
TTCTGATGCCTTCAACATGT+TGG - chr4_2:16703383-16703402 Msa0591820:CDS 40.0%
TGATGCTCATATGCCAAACA+TGG - chr4_2:16703437-16703456 Msa0591820:intron 40.0%
CGAATATTGGACTAAGCCTT+TGG - chr4_2:16703665-16703684 Msa0591820:intron 40.0%
AAAACGAACCTGTAAATGGC+TGG + chr4_2:16704069-16704088 None:intergenic 40.0%
AAACCTAGGCAACTAGACTT+AGG + chr4_2:16704132-16704151 None:intergenic 40.0%
GAGTAGCTTCAGCTACTTAT+AGG - chr4_2:16704253-16704272 Msa0591820:CDS 40.0%
CAGGTTCGTTTCATTTACAC+CGG + chr4_2:16704279-16704298 None:intergenic 40.0%
AGAAGCTACTCACTCAATAC+CGG - chr4_2:16704637-16704656 Msa0591820:intron 40.0%
ATTGCGATGCACAACAACAT+TGG - chr4_2:16704708-16704727 Msa0591820:intron 40.0%
TTGCGATGCACAACAACATT+GGG - chr4_2:16704709-16704728 Msa0591820:intron 40.0%
CAACAACATTGGGTGGAAAT+TGG - chr4_2:16704719-16704738 Msa0591820:CDS 40.0%
CACTCTCCTTAATTTCGTCG+AGG - chr4_2:16703209-16703228 Msa0591820:CDS 45.0%
ATCAGAAGCCATGCTGCATT+TGG + chr4_2:16703370-16703389 None:intergenic 45.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4_2:16703652-16703671 Msa0591820:intron 45.0%
ATTCTCGCGACCAAGACAAA+CGG + chr4_2:16703848-16703867 None:intergenic 45.0%
CTACGTGCAGTCAATCAACT+AGG - chr4_2:16703882-16703901 Msa0591820:intron 45.0%
ACATGTTGCCAGCCATTTAC+AGG - chr4_2:16704058-16704077 Msa0591820:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG - chr4_2:16704126-16704145 Msa0591820:CDS 45.0%
AGCTTCAGCTACTTATAGGC+CGG - chr4_2:16704257-16704276 Msa0591820:CDS 45.0%
AGCTCAACCGATGAGACAAA+AGG - chr4_2:16704566-16704585 Msa0591820:CDS 45.0%
AGAAAACTTTGGTCCTGCTC+CGG + chr4_2:16704659-16704678 None:intergenic 45.0%
CACTCTCCTTAATTTCGTCG+AGG - chr4_2:16703209-16703228 Msa0591820:CDS 45.0%
ATCAGAAGCCATGCTGCATT+TGG + chr4_2:16703370-16703389 None:intergenic 45.0%
AATGTGGGGCTTGCGAATAT+TGG - chr4_2:16703652-16703671 Msa0591820:intron 45.0%
ATTCTCGCGACCAAGACAAA+CGG + chr4_2:16703848-16703867 None:intergenic 45.0%
CTACGTGCAGTCAATCAACT+AGG - chr4_2:16703882-16703901 Msa0591820:intron 45.0%
ACATGTTGCCAGCCATTTAC+AGG - chr4_2:16704058-16704077 Msa0591820:CDS 45.0%
GTACCTAAGTCTAGTTGCCT+AGG - chr4_2:16704126-16704145 Msa0591820:CDS 45.0%
AGCTTCAGCTACTTATAGGC+CGG - chr4_2:16704257-16704276 Msa0591820:CDS 45.0%
AGCTCAACCGATGAGACAAA+AGG - chr4_2:16704566-16704585 Msa0591820:CDS 45.0%
AGAAAACTTTGGTCCTGCTC+CGG + chr4_2:16704659-16704678 None:intergenic 45.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4_2:16703835-16703854 Msa0591820:intron 50.0%
TTCTCGCGACCAAGACAAAC+GGG + chr4_2:16703847-16703866 None:intergenic 50.0%
TACTCACTCAATACCGGAGC+AGG - chr4_2:16704643-16704662 Msa0591820:intron 50.0%
CGATGCACAACAACATTGGG+TGG - chr4_2:16704712-16704731 Msa0591820:CDS 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG - chr4_2:16703835-16703854 Msa0591820:intron 50.0%
TTCTCGCGACCAAGACAAAC+GGG + chr4_2:16703847-16703866 None:intergenic 50.0%
TACTCACTCAATACCGGAGC+AGG - chr4_2:16704643-16704662 Msa0591820:intron 50.0%
CGATGCACAACAACATTGGG+TGG - chr4_2:16704712-16704731 Msa0591820:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 16703141 16704829 16703141 ID=Msa0591820;Name=Msa0591820
chr4_2 mRNA 16703141 16704829 16703141 ID=Msa0591820-mRNA-1;Parent=Msa0591820;Name=Msa0591820-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0|0|1|1|1|5|0|311
chr4_2 exon 16704712 16704829 16704712 ID=Msa0591820-mRNA-1:exon:4852;Parent=Msa0591820-mRNA-1
chr4_2 exon 16704457 16704606 16704457 ID=Msa0591820-mRNA-1:exon:4851;Parent=Msa0591820-mRNA-1
chr4_2 exon 16704058 16704363 16704058 ID=Msa0591820-mRNA-1:exon:4850;Parent=Msa0591820-mRNA-1
chr4_2 exon 16703891 16703967 16703891 ID=Msa0591820-mRNA-1:exon:4849;Parent=Msa0591820-mRNA-1
chr4_2 exon 16703141 16703425 16703141 ID=Msa0591820-mRNA-1:exon:4848;Parent=Msa0591820-mRNA-1
chr4_2 CDS 16704712 16704829 16704712 ID=Msa0591820-mRNA-1:cds;Parent=Msa0591820-mRNA-1
chr4_2 CDS 16704457 16704606 16704457 ID=Msa0591820-mRNA-1:cds;Parent=Msa0591820-mRNA-1
chr4_2 CDS 16704058 16704363 16704058 ID=Msa0591820-mRNA-1:cds;Parent=Msa0591820-mRNA-1
chr4_2 CDS 16703891 16703967 16703891 ID=Msa0591820-mRNA-1:cds;Parent=Msa0591820-mRNA-1
chr4_2 CDS 16703141 16703425 16703141 ID=Msa0591820-mRNA-1:cds;Parent=Msa0591820-mRNA-1
Gene Sequence

>Msa0591820

ATGGCCCTTTTCACCGACAAACCATCATTATCAGAAGGTCTCAAGGTTCTTGCAATTGATCATGACATCACTCTCCTTAATTTCGTCGAGGAGATGTGTAATCGATGCAATTATAAGGTTACCAAATGCAGCATGGCTTCTGATGCCTTCAACATGTTGGAAAGAAAGGACTGTTTCGATGTAATATTGATTGATGCTCATATGCCAAACATGGATGTTTACGACTTTGTGCAAAATGTTACCCTACAACTCAAAATTCCTGTCATTATGATGGCCGTTGATTCTGCAGAAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTGGTTGAAAAACAGTTCAAGACCATGTGGCAACATGTTATAAGAAAAGGTTTGACTCAAAACAAAAAATATGAAATTGTTGGAAGCTCGGTGTTTCAAGAAAATAGAAAACGGGGTAGAGAAGATGCTAATGCCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCGCGAGAATTGCATCAGCAGTTTCTACGTGCAGTCAATCAACTAGGCCTTGATAGGGCAACGCCAAAAAAGATTCTGAAAATCATGGATGTTCCCGATATGAAAAAAGAACATGTTGCCAGCCATTTACAGAAGTATAGAAATCAGTTTAAGAGCTCAACCGATGAGACAAAAGGTAAGATGAAAAAATCAAAGAAGAGTCCTCTTGAAACACAATTTAGCTTAGAAGCTACTCACTCAATACCGGAGCAGGACCAAAGTTTTCTTCATAATTCTGCGACACAATGTCGCGACAATTGCGATGCACAACAACATTGGGTGGAAATTGGTGAATCCAACATTATGAGCAACACTTTTTCTGACTTTCCCAACTTGTTCCCATATGTTGATGATGATTTATCATCACTTGTGTGGTGA
Protein sequence

>Msa0591820

MALFTDKPSLSEGLKVLAIDHDITLLNFVEEMCNRCNYKVTKCSMASDAFNMLERKDCFDVILIDAHMPNMDVYDFVQNVTLQLKIPVIMMAVDSAESSIMKSIECGACEYWTKPLVEKQFKTMWQHVIRKGLTQNKKYEIVGSSVFQENRKRGREDANASKETLAKKARLSWSRELHQQFLRAVNQLGLDRATPKKILKIMDVPDMKKEHVASHLQKYRNQFKSSTDETKGKMKKSKKSPLETQFSLEATHSIPEQDQSFLHNSATQCRDNCDAQQHWVEIGESNIMSNTFSDFPNLFPYVDDDLSSLVW*