AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0591880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0591880 MtrunA17_Chr4g0009901 87.226 274 35 0 1 274 1 274 0.0 501
Msa0591880 MtrunA17_Chr4g0009851 79.253 241 49 1 1 240 1 241 8.56e-138 390
Msa0591880 MtrunA17_Chr4g0009981 77.128 188 43 0 53 240 1 188 1.76e-102 299
Msa0591880 MtrunA17_Chr4g0011071 79.851 134 27 0 58 191 38 171 6.44e-77 231
Msa0591880 MtrunA17_Chr1g0161431 45.749 247 103 3 10 225 11 257 5.69e-68 221
Msa0591880 MtrunA17_Chr4g0009991 82.051 117 20 1 101 217 1 116 2.94e-63 194
Msa0591880 MtrunA17_Chr4g0067981 39.759 249 124 2 10 232 31 279 2.45e-56 191
Msa0591880 MtrunA17_Chr2g0294911 40.833 240 118 2 10 225 29 268 1.26e-55 188
Msa0591880 MtrunA17_Chr6g0471041 45.570 237 118 4 10 244 14 241 3.24e-55 183
Msa0591880 MtrunA17_Chr3g0123131 39.313 262 129 4 10 241 13 274 4.29e-55 186
Msa0591880 MtrunA17_Chr8g0376881 39.918 243 120 3 9 225 24 266 6.09e-54 182
Msa0591880 MtrunA17_Chr4g0010001 84.270 89 14 0 1 89 1 89 7.32e-53 167
Msa0591880 MtrunA17_Chr8g0391911 40.486 247 121 3 10 230 9 255 4.52e-52 171
Msa0591880 MtrunA17_Chr5g0423031 84.270 89 14 0 1 89 1 89 2.04e-50 164
Msa0591880 MtrunA17_Chr5g0423031 59.259 81 31 1 184 262 80 160 5.38e-18 79.7
Msa0591880 MtrunA17_Chr1g0149841 39.271 247 122 3 7 225 2 248 2.25e-49 170
Msa0591880 MtrunA17_Chr7g0275401 37.200 250 128 4 7 227 2 251 2.79e-47 159
Msa0591880 MtrunA17_Chr8g0383961 43.590 234 101 7 1 229 1 208 4.99e-46 155
Msa0591880 MtrunA17_Chr3g0137101 36.694 248 125 3 10 225 8 255 6.64e-45 160
Msa0591880 MtrunA17_Chr7g0275891 35.521 259 126 5 7 224 2 260 1.16e-44 154
Msa0591880 MtrunA17_Chr6g0456631 46.405 153 80 1 10 160 9 161 1.09e-43 146
Msa0591880 MtrunA17_Chr4g0074401 34.661 251 134 5 10 231 17 266 7.90e-42 151
Msa0591880 MtrunA17_Chr1g0149851 35.094 265 129 5 7 228 2 266 8.69e-42 150
Msa0591880 MtrunA17_Chr4g0074411 34.818 247 136 4 10 231 18 264 2.25e-41 150
Msa0591880 MtrunA17_Chr2g0304841 32.677 254 134 3 10 226 18 271 1.64e-40 148
Msa0591880 MtrunA17_Chr3g0134461 35.714 210 108 4 10 217 8 192 8.20e-35 124
Msa0591880 MtrunA17_Chr3g0134481 34.597 211 102 3 10 219 8 183 4.05e-34 122
Msa0591880 MtrunA17_Chr4g0074421 35.912 181 94 3 59 217 2 182 1.44e-32 118
Msa0591880 MtrunA17_Chr1g0149831 37.297 185 86 3 68 225 1 182 4.29e-30 112
Msa0591880 MtrunA17_Chr6g0473731 34.416 154 89 2 89 233 10 160 1.64e-19 87.8
Msa0591880 MtrunA17_Chr7g0223971 32.620 187 74 5 91 229 1 183 1.85e-17 82.0
Msa0591880 MtrunA17_Chr5g0417521 61.818 55 20 1 168 221 15 69 2.54e-17 80.5
Msa0591880 MtrunA17_Chr6g0461641 63.636 55 19 1 168 221 42 96 8.54e-17 79.3
Msa0591880 MtrunA17_Chr2g0321461 55.072 69 29 2 154 221 32 99 5.15e-16 77.0
Msa0591880 MtrunA17_Chr4g0031961 50.725 69 34 0 157 225 120 188 5.88e-15 73.6
Msa0591880 MtrunA17_Chr7g0261911 48.052 77 39 1 161 236 255 331 9.58e-15 73.9
Msa0591880 MtrunA17_Chr2g0324891 42.553 94 51 2 133 224 125 217 1.09e-14 73.6
Msa0591880 MtrunA17_Chr7g0254981 44.318 88 46 2 159 243 41 128 1.23e-14 72.4
Msa0591880 MtrunA17_Chr7g0255711 55.932 59 26 0 167 225 90 148 2.06e-14 71.6
Msa0591880 MtrunA17_Chr7g0274471 52.857 70 30 2 168 234 218 287 3.58e-14 72.0
Msa0591880 MtrunA17_Chr4g0042451 56.364 55 23 1 168 221 17 71 3.89e-14 71.2
Msa0591880 MtrunA17_Chr1g0190301 44.681 94 48 3 157 249 165 255 8.23e-14 70.9
Msa0591880 MtrunA17_Chr4g0040481 55.172 58 26 0 167 224 157 214 1.42e-13 70.1
Msa0591880 MtrunA17_Chr2g0283951 43.902 82 40 3 150 229 75 152 2.02e-13 69.7
Msa0591880 MtrunA17_Chr1g0198601 47.500 80 34 2 149 227 199 271 2.15e-13 69.3
Msa0591880 MtrunA17_Chr1g0162931 50.000 68 32 2 154 220 5 71 4.30e-13 67.8
Msa0591880 MtrunA17_Chr4g0054981 55.357 56 25 0 167 222 309 364 5.26e-13 68.6
Msa0591880 MtrunA17_Chr3g0095611 51.562 64 30 1 158 220 9 72 5.77e-13 66.2
Msa0591880 MtrunA17_Chr1g0175091 50.847 59 28 1 164 221 254 312 6.76e-13 68.2
Msa0591880 MtrunA17_Chr7g0243081 48.438 64 32 1 158 220 61 124 8.18e-13 67.8
Msa0591880 MtrunA17_Chr5g0400241 52.542 59 28 0 167 225 310 368 1.33e-12 67.4
Msa0591880 MtrunA17_Chr7g0258551 52.632 57 26 1 165 220 209 265 1.46e-12 67.0
Msa0591880 MtrunA17_Chr2g0291021 53.425 73 30 3 168 236 49 121 1.94e-12 66.2
Msa0591880 MtrunA17_Chr4g0062921 46.753 77 39 2 158 233 226 301 2.71e-12 66.2
Msa0591880 MtrunA17_Chr2g0320231 44.595 74 37 2 148 220 80 150 4.63e-12 65.5
Msa0591880 MtrunA17_Chr8g0356501 54.237 59 26 1 164 221 33 91 4.65e-12 65.5
Msa0591880 MtrunA17_Chr8g0379121 44.776 67 36 1 155 220 98 164 5.38e-12 65.5
Msa0591880 MtrunA17_Chr1g0210911 47.143 70 34 2 154 220 6 75 6.88e-12 64.7
Msa0591880 MtrunA17_Chr2g0310011 54.237 59 26 1 164 221 31 89 6.92e-12 64.7
Msa0591880 MtrunA17_Chr1g0175071 54.098 61 27 1 164 223 173 233 1.24e-11 63.9
Msa0591880 MtrunA17_Chr1g0197171 56.364 55 23 1 168 221 44 98 1.63e-11 63.5
Msa0591880 MtrunA17_Chr5g0422621 52.459 61 28 1 167 226 197 257 1.75e-11 63.5
Msa0591880 MtrunA17_Chr4g0045361 50.820 61 29 1 166 225 272 332 1.79e-11 63.9
Msa0591880 MtrunA17_Chr5g0409731 47.692 65 31 2 168 229 25 89 2.77e-11 62.8
Msa0591880 MtrunA17_Chr2g0288601 52.830 53 24 1 166 217 69 121 4.42e-11 59.3
Msa0591880 MtrunA17_Chr2g0290991 48.485 66 33 1 164 228 35 100 4.46e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0591880 AT2G01760.1 38.489 278 130 6 7 245 4 279 3.85e-56 185
Msa0591880 AT1G67710.1 39.773 264 128 6 11 243 8 271 6.99e-51 174
Msa0591880 AT2G01760.2 34.967 306 130 7 7 245 4 307 3.64e-50 170
Msa0591880 AT3G16857.1 37.918 269 121 5 10 232 33 301 1.26e-49 174
Msa0591880 AT3G16857.2 37.918 269 121 5 10 232 33 301 1.79e-49 173
Msa0591880 AT2G25180.1 34.859 284 155 6 10 268 13 291 3.38e-44 158
Msa0591880 AT4G31920.1 36.481 233 136 3 10 230 13 245 8.80e-44 156
Msa0591880 AT2G01760.3 37.500 216 95 5 68 245 1 214 1.38e-37 135
Msa0591880 AT5G58080.1 37.037 243 121 6 10 225 14 251 1.77e-37 140
Msa0591880 AT3G16857.3 36.522 230 100 5 49 232 2 231 2.33e-37 139
Msa0591880 AT1G67710.2 41.398 186 81 4 68 225 1 186 1.61e-34 130
Msa0591880 AT3G62670.1 33.333 222 125 6 28 226 53 274 2.83e-30 117
Msa0591880 AT5G49240.1 30.508 236 137 4 9 217 37 272 3.14e-30 115
Msa0591880 AT1G49190.1 31.200 250 137 8 10 226 30 277 6.08e-30 116
Msa0591880 AT1G67710.3 39.247 186 83 5 88 243 12 197 2.66e-26 107
Msa0591880 AT5G07210.2 28.148 270 138 7 14 229 16 283 1.01e-23 100
Msa0591880 AT5G07210.1 27.338 278 138 8 14 229 16 291 3.23e-21 93.6
Msa0591880 AT1G68210.1 25.993 277 142 7 8 225 7 279 3.57e-18 84.7
Msa0591880 AT1G49190.2 30.709 127 83 2 10 133 30 154 1.04e-16 80.1
Msa0591880 AT5G29000.4 42.157 102 51 3 120 220 190 284 1.34e-16 78.6
Msa0591880 AT5G29000.1 42.157 102 51 3 120 220 147 241 2.26e-16 78.6
Msa0591880 AT5G29000.3 42.157 102 51 3 120 220 147 241 2.26e-16 78.6
Msa0591880 AT5G18240.2 55.556 72 30 2 151 221 29 99 2.29e-16 78.6
Msa0591880 AT5G18240.3 55.556 72 30 2 151 221 29 99 2.29e-16 78.6
Msa0591880 AT5G18240.1 55.556 72 30 2 151 221 29 99 2.38e-16 78.6
Msa0591880 AT5G18240.4 55.556 72 30 2 151 221 29 99 2.38e-16 78.6
Msa0591880 AT5G29000.2 41.748 103 52 3 120 221 190 285 2.49e-16 78.6
Msa0591880 AT3G04030.1 61.818 55 20 1 168 221 45 99 2.67e-16 78.6
Msa0591880 AT5G18240.5 55.556 72 30 2 151 221 29 99 2.72e-16 78.6
Msa0591880 AT3G04030.3 61.818 55 20 1 168 221 45 99 2.83e-16 78.6
Msa0591880 AT2G27070.1 25.651 269 141 8 14 225 16 282 7.23e-16 77.8
Msa0591880 AT3G46640.3 44.681 94 49 2 144 237 123 213 2.91e-15 75.1
Msa0591880 AT3G04030.2 57.407 54 23 0 168 221 45 98 3.01e-15 75.5
Msa0591880 AT3G46640.2 44.681 94 49 2 144 237 123 213 3.41e-15 74.7
Msa0591880 AT3G46640.1 44.681 94 49 2 144 237 123 213 3.41e-15 74.7
Msa0591880 AT5G05090.1 55.932 59 26 0 167 225 80 138 8.84e-15 72.8
Msa0591880 AT5G59570.2 53.333 60 28 0 166 225 139 198 1.05e-14 73.2
Msa0591880 AT5G59570.1 53.333 60 28 0 166 225 139 198 1.05e-14 73.2
Msa0591880 AT3G10760.1 55.932 59 26 0 167 225 104 162 1.88e-14 72.8
Msa0591880 AT2G40970.1 54.237 59 27 0 167 225 104 162 2.80e-14 71.2
Msa0591880 AT3G04450.1 53.448 58 26 1 164 220 234 291 3.10e-14 72.4
Msa0591880 AT3G04450.4 53.448 58 26 1 164 220 194 251 3.25e-14 72.4
Msa0591880 AT3G04450.2 53.448 58 26 1 164 220 194 251 3.25e-14 72.4
Msa0591880 AT5G44190.1 56.667 60 26 0 165 224 144 203 3.89e-14 72.0
Msa0591880 AT3G04450.3 53.448 58 26 1 164 220 234 291 4.04e-14 71.6
Msa0591880 AT5G44190.2 56.667 60 26 0 165 224 144 203 4.60e-14 71.6
Msa0591880 AT2G01060.1 52.703 74 33 2 166 238 13 85 5.80e-14 70.9
Msa0591880 AT4G28610.1 60.000 55 21 1 168 221 225 279 1.46e-13 70.5
Msa0591880 AT2G40260.1 41.584 101 52 3 134 233 51 145 2.17e-13 70.1
Msa0591880 AT3G24120.1 38.235 102 54 2 164 256 37 138 2.56e-13 68.9
Msa0591880 AT3G24120.2 38.235 102 54 2 164 256 37 138 2.65e-13 68.9
Msa0591880 AT1G69580.1 49.231 65 32 1 168 231 34 98 3.16e-13 69.3
Msa0591880 AT1G69580.2 49.231 65 32 1 168 231 34 98 3.37e-13 68.9
Msa0591880 AT4G13640.2 40.594 101 41 3 168 267 37 119 3.55e-13 68.6
Msa0591880 AT4G13640.1 40.594 101 41 3 168 267 37 119 3.70e-13 68.6
Msa0591880 AT2G20570.1 49.275 69 35 0 156 224 141 209 5.11e-13 68.9
Msa0591880 AT1G14600.1 61.538 52 19 1 170 220 25 76 1.24e-12 66.6
Msa0591880 AT1G25550.1 54.839 62 27 1 167 227 208 269 1.66e-12 67.0
Msa0591880 AT3G12730.1 34.746 118 73 3 149 264 3 118 1.68e-12 65.9
Msa0591880 AT5G06800.3 58.182 55 22 1 168 221 192 246 1.80e-12 66.6
Msa0591880 AT5G06800.1 51.471 68 30 2 168 234 192 257 1.90e-12 67.0
Msa0591880 AT1G79430.2 42.857 91 46 2 164 253 30 115 2.18e-12 66.6
Msa0591880 AT5G06800.2 58.182 55 22 1 168 221 192 246 3.03e-12 66.6
Msa0591880 AT4G04605.1 45.333 75 39 2 166 238 12 86 3.94e-12 64.7
Msa0591880 AT3G13040.3 53.571 56 25 1 167 221 240 295 4.02e-12 66.2
Msa0591880 AT3G13040.1 53.571 56 25 1 167 221 240 295 4.02e-12 66.2
Msa0591880 AT3G13040.2 53.571 56 25 1 167 221 240 295 4.02e-12 66.2
Msa0591880 AT2G20570.2 58.182 55 23 0 170 224 171 225 5.05e-12 65.9
Msa0591880 AT4G18020.8 50.000 66 32 1 159 224 288 352 5.44e-12 65.9
Msa0591880 AT4G18020.11 50.000 66 32 1 159 224 288 352 5.44e-12 65.9
Msa0591880 AT4G18020.4 50.000 66 32 1 159 224 288 352 5.44e-12 65.9
Msa0591880 AT4G18020.5 50.000 66 32 1 159 224 288 352 5.44e-12 65.9
Msa0591880 AT4G18020.10 50.000 66 32 1 159 224 288 352 5.44e-12 65.9
Msa0591880 AT4G18020.7 50.000 66 32 1 159 224 288 352 5.44e-12 65.9
Msa0591880 AT4G18020.3 50.000 66 32 1 159 224 288 352 5.88e-12 65.9
Msa0591880 AT4G18020.2 50.000 66 32 1 159 224 288 352 5.88e-12 65.9
Msa0591880 AT4G18020.1 50.000 66 32 1 159 224 288 352 5.88e-12 65.9
Msa0591880 AT4G18020.6 50.000 66 32 1 159 224 288 352 5.88e-12 65.9
Msa0591880 AT4G18020.9 50.000 66 32 1 159 224 288 352 5.88e-12 65.9
Msa0591880 AT2G20400.1 59.259 54 21 1 168 220 231 284 8.61e-12 65.1
Msa0591880 AT2G20400.2 59.259 54 21 1 168 220 231 284 8.79e-12 65.1
Msa0591880 AT2G03500.1 50.000 62 30 1 167 227 232 293 1.63e-11 64.3
Msa0591880 AT2G42660.1 45.312 64 34 1 158 220 40 103 2.63e-11 62.8
Msa0591880 AT5G45580.1 54.545 55 24 1 168 221 7 61 3.31e-11 62.8
Msa0591880 AT5G45580.2 42.105 76 43 1 168 242 23 98 3.78e-11 62.8
Msa0591880 AT2G38300.1 48.214 56 28 1 166 220 52 107 5.08e-11 62.8
Msa0591880 AT2G02060.2 55.769 52 22 1 170 220 31 82 9.70e-11 61.2
Msa0591880 AT2G02060.1 55.769 52 22 1 170 220 31 82 9.70e-11 61.2

Find 67 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTTTCCAACAAATTAA+AGG 0.224235 4_2:-16844862 None:intergenic
AATTGCATCAACGATTTCTA+TGG 0.228648 4_2:+16845350 Msa0591880:CDS
CAAAATGATTCTCAAAATTA+TGG 0.243357 4_2:+16845497 Msa0591880:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 4_2:+16845542 Msa0591880:CDS
CTCAAAATTATGGATGTTCC+TGG 0.317981 4_2:+16845507 Msa0591880:CDS
AGAAATCGTTGATGCAATTC+TGG 0.327125 4_2:-16845346 None:intergenic
CTTTAATTTGTTGGAAAGAA+TGG 0.333344 4_2:+16844863 Msa0591880:CDS
TTCTGATGCCTTTAATTTGT+TGG 0.335924 4_2:+16844854 Msa0591880:CDS
CTAAGAATTCTTTAATTAAA+CGG 0.349796 4_2:-16845862 None:intergenic
TGAGTTGTAGGGTAACATTT+TGG 0.376494 4_2:-16844947 None:intergenic
AAAGTGACTAACCTTGATAA+TGG 0.378712 4_2:-16844696 None:intergenic
CTGAATGGATTGATCAAAAT+CGG 0.378920 4_2:+16845767 Msa0591880:CDS
AATGTGGGGCTTGCGAATAT+TGG 0.385194 4_2:+16845137 Msa0591880:CDS
AATTATGGATGTTCCTGGTT+TGG 0.386251 4_2:+16845512 Msa0591880:CDS
ATTATGGATGTTCCTGGTTT+GGG 0.388266 4_2:+16845513 Msa0591880:CDS
ATCCTACCTTCACAACCTTA+TGG 0.394112 4_2:+16845831 Msa0591880:CDS
TCTTTAATTAAACGGCATCT+AGG 0.403700 4_2:-16845854 None:intergenic
TGACAGGAATGTTGAGTTGT+AGG 0.408636 4_2:-16844959 None:intergenic
ATCAGAAGCTGTGTTACATT+TGG 0.412873 4_2:-16844838 None:intergenic
AATAAAGAATATGAAATTGT+TGG 0.416766 4_2:+16845229 Msa0591880:CDS
CAAAGAAGGCCCGTTTGTCT+TGG 0.418636 4_2:+16845320 Msa0591880:CDS
TGATCAAGAAAATAGAAAAC+GGG 0.418887 4_2:+16845261 Msa0591880:CDS
TGGCAACATGTTATAAGAAA+AGG 0.422808 4_2:+16845196 Msa0591880:CDS
AATGTCGTTTGCAGAAATAT+AGG 0.426550 4_2:+16845671 Msa0591880:intron
ATATGAAATTGTTGGAAGCT+TGG 0.432524 4_2:+16845237 Msa0591880:CDS
AAAAGAAACACTTGCAAAGA+AGG 0.435703 4_2:+16845306 Msa0591880:CDS
TGTAATCGATTCCATTATCA+AGG 0.450836 4_2:+16844685 Msa0591880:CDS
AAGAAATTCTTACTAATGAC+AGG 0.461835 4_2:-16844975 None:intergenic
AAGTCATAAACATCCATGTT+TGG 0.465091 4_2:-16844921 None:intergenic
TTGAGACCAGCAGGGAATGA+TGG 0.467536 4_2:-16844610 None:intergenic
TTCTGGCGACCAAGACAAAC+GGG 0.468019 4_2:-16845329 None:intergenic
TTGATCAAGAAAATAGAAAA+CGG 0.477078 4_2:+16845260 Msa0591880:CDS
ACGATGACTTTCTAGCTGAA+TGG 0.481545 4_2:+16845791 Msa0591880:CDS
GATGTTCCTGGTTTGGGTGT+AGG 0.487511 4_2:+16845519 Msa0591880:CDS
GAATGGATTGACTGTGGGGC+GGG 0.487587 4_2:+16845808 Msa0591880:CDS
TAGCTGAATGGATTGACTGT+GGG 0.492334 4_2:+16845803 Msa0591880:CDS
CACTTTCCTCAACTCTATTG+AGG 0.492388 4_2:+16844657 Msa0591880:CDS
GCAATAACCTTGAGACCAGC+AGG 0.494358 4_2:-16844619 None:intergenic
TAAACGGCATCTAGGCCATA+AGG 0.496018 4_2:-16845846 None:intergenic
ATTCTGGCGACCAAGACAAA+CGG 0.509916 4_2:-16845330 None:intergenic
ATTATGAAGTCTATTGAATG+TGG 0.513075 4_2:+16845121 Msa0591880:CDS
TAGGATTCATTTGAAGAGAT+CGG 0.515087 4_2:+16845690 Msa0591880:CDS
GTGATAGCTCTCAAGCTGAA+TGG 0.519299 4_2:+16845752 Msa0591880:CDS
GACAGGAATGTTGAGTTGTA+GGG 0.519779 4_2:-16844958 None:intergenic
ATCATTCCCTGCTGGTCTCA+AGG 0.520371 4_2:+16844612 Msa0591880:CDS
AGGAAAGAAGCATACTATCA+AGG 0.524721 4_2:-16845390 None:intergenic
TTATGAAGTCTATTGAATGT+GGG 0.532174 4_2:+16845122 Msa0591880:CDS
GACAACCCATCATTCCCTGC+TGG 0.539365 4_2:+16844604 Msa0591880:CDS
TGAGACCAGCAGGGAATGAT+GGG 0.539790 4_2:-16844609 None:intergenic
CTATGGGCAGTCAATCAACT+AGG 0.548837 4_2:+16845367 Msa0591880:CDS
CTTCATAATAGAACTTTCTG+TGG 0.551255 4_2:-16845107 None:intergenic
ATTGCATCAACGATTTCTAT+GGG 0.557361 4_2:+16845351 Msa0591880:CDS
CTAGCTGAATGGATTGACTG+TGG 0.582882 4_2:+16845802 Msa0591880:CDS
GAAAGTGATGTCATGATCAA+TGG 0.586165 4_2:-16844641 None:intergenic
TCTTATAACATGTTGCCACA+TGG 0.594344 4_2:-16845191 None:intergenic
TATGAAGTCTATTGAATGTG+GGG 0.598969 4_2:+16845123 Msa0591880:CDS
ACATGTCCTACACCCAAACC+AGG 0.611300 4_2:-16845525 None:intergenic
GGCCATAAGGTTGTGAAGGT+AGG 0.618309 4_2:-16845833 None:intergenic
AGCTGAATGGATTGACTGTG+GGG 0.620097 4_2:+16845804 Msa0591880:CDS
TCTAGGCCATAAGGTTGTGA+AGG 0.622701 4_2:-16845837 None:intergenic
ACATCTCCTCAATAGAGTTG+AGG 0.625514 4_2:-16844663 None:intergenic
TAATGCTCATATGCCAAACA+TGG 0.628020 4_2:+16844908 Msa0591880:CDS
TGAATGGATTGACTGTGGGG+CGG 0.657142 4_2:+16845807 Msa0591880:CDS
CGGCTGATGAAACAAAACAC+GGG 0.673622 4_2:+16845710 Msa0591880:CDS
CAATAACCTTGAGACCAGCA+GGG 0.699486 4_2:-16844618 None:intergenic
TCGGCTGATGAAACAAAACA+CGG 0.707340 4_2:+16845709 Msa0591880:CDS
GATCAAGAAAATAGAAAACG+GGG 0.722642 4_2:+16845262 Msa0591880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAGAATATGAAATTGT+TGG + chr4_2:16845229-16845248 Msa0591880:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4_2:16845498-16845517 None:intergenic 15.0%
!! AATAAAGAATATGAAATTGT+TGG + chr4_2:16845229-16845248 Msa0591880:CDS 15.0%
!!! ATAATTTTGAGAATCATTTT+GGG - chr4_2:16845498-16845517 None:intergenic 15.0%
!! TTGATCAAGAAAATAGAAAA+CGG + chr4_2:16845260-16845279 Msa0591880:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4_2:16845499-16845518 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4_2:16845497-16845516 Msa0591880:CDS 20.0%
!! ATAATTTAATTAGACTCCTT+CGG - chr4_2:16845576-16845595 None:intergenic 20.0%
!! TTGATCAAGAAAATAGAAAA+CGG + chr4_2:16845260-16845279 Msa0591880:CDS 20.0%
!!! CATAATTTTGAGAATCATTT+TGG - chr4_2:16845499-16845518 None:intergenic 20.0%
!! CAAAATGATTCTCAAAATTA+TGG + chr4_2:16845497-16845516 Msa0591880:CDS 20.0%
!! ATAATTTAATTAGACTCCTT+CGG - chr4_2:16845576-16845595 None:intergenic 20.0%
! CATTCTTTCCAACAAATTAA+AGG - chr4_2:16844865-16844884 None:intergenic 25.0%
!!! CTTTAATTTGTTGGAAAGAA+TGG + chr4_2:16844863-16844882 Msa0591880:CDS 25.0%
! AAGAAATTCTTACTAATGAC+AGG - chr4_2:16844978-16844997 None:intergenic 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_2:16845075-16845094 Msa0591880:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_2:16845121-16845140 Msa0591880:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_2:16845122-16845141 Msa0591880:CDS 25.0%
!!! TTGAACTGTTTTTCAACAAA+AGG - chr4_2:16845169-16845188 None:intergenic 25.0%
! TGATCAAGAAAATAGAAAAC+GGG + chr4_2:16845261-16845280 Msa0591880:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4_2:16845413-16845432 None:intergenic 25.0%
! CATTCTTTCCAACAAATTAA+AGG - chr4_2:16844865-16844884 None:intergenic 25.0%
!!! CTTTAATTTGTTGGAAAGAA+TGG + chr4_2:16844863-16844882 Msa0591880:CDS 25.0%
! AAGAAATTCTTACTAATGAC+AGG - chr4_2:16844978-16844997 None:intergenic 25.0%
!!! TTATTTCTCTTTTGTAGTGA+TGG + chr4_2:16845075-16845094 Msa0591880:intron 25.0%
! ATTATGAAGTCTATTGAATG+TGG + chr4_2:16845121-16845140 Msa0591880:CDS 25.0%
! TTATGAAGTCTATTGAATGT+GGG + chr4_2:16845122-16845141 Msa0591880:CDS 25.0%
!!! TTGAACTGTTTTTCAACAAA+AGG - chr4_2:16845169-16845188 None:intergenic 25.0%
! TGATCAAGAAAATAGAAAAC+GGG + chr4_2:16845261-16845280 Msa0591880:CDS 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG - chr4_2:16845413-16845432 None:intergenic 25.0%
! TGTAATCGATTCCATTATCA+AGG + chr4_2:16844685-16844704 Msa0591880:CDS 30.0%
AAAGTGACTAACCTTGATAA+TGG - chr4_2:16844699-16844718 None:intergenic 30.0%
!! TTCTGATGCCTTTAATTTGT+TGG + chr4_2:16844854-16844873 Msa0591880:CDS 30.0%
AAGTCATAAACATCCATGTT+TGG - chr4_2:16844924-16844943 None:intergenic 30.0%
CTTCATAATAGAACTTTCTG+TGG - chr4_2:16845110-16845129 None:intergenic 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_2:16845123-16845142 Msa0591880:CDS 30.0%
TGGCAACATGTTATAAGAAA+AGG + chr4_2:16845196-16845215 Msa0591880:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4_2:16845237-16845256 Msa0591880:CDS 30.0%
GATCAAGAAAATAGAAAACG+GGG + chr4_2:16845262-16845281 Msa0591880:CDS 30.0%
AAAAGAAACACTTGCAAAGA+AGG + chr4_2:16845306-16845325 Msa0591880:CDS 30.0%
AATTGCATCAACGATTTCTA+TGG + chr4_2:16845350-16845369 Msa0591880:CDS 30.0%
ATTGCATCAACGATTTCTAT+GGG + chr4_2:16845351-16845370 Msa0591880:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4_2:16845671-16845690 Msa0591880:intron 30.0%
TAGGATTCATTTGAAGAGAT+CGG + chr4_2:16845690-16845709 Msa0591880:CDS 30.0%
!! CTGAATGGATTGATCAAAAT+CGG + chr4_2:16845767-16845786 Msa0591880:CDS 30.0%
! TGTAATCGATTCCATTATCA+AGG + chr4_2:16844685-16844704 Msa0591880:CDS 30.0%
AAAGTGACTAACCTTGATAA+TGG - chr4_2:16844699-16844718 None:intergenic 30.0%
!! TTCTGATGCCTTTAATTTGT+TGG + chr4_2:16844854-16844873 Msa0591880:CDS 30.0%
AAGTCATAAACATCCATGTT+TGG - chr4_2:16844924-16844943 None:intergenic 30.0%
CTTCATAATAGAACTTTCTG+TGG - chr4_2:16845110-16845129 None:intergenic 30.0%
TATGAAGTCTATTGAATGTG+GGG + chr4_2:16845123-16845142 Msa0591880:CDS 30.0%
TGGCAACATGTTATAAGAAA+AGG + chr4_2:16845196-16845215 Msa0591880:CDS 30.0%
ATATGAAATTGTTGGAAGCT+TGG + chr4_2:16845237-16845256 Msa0591880:CDS 30.0%
GATCAAGAAAATAGAAAACG+GGG + chr4_2:16845262-16845281 Msa0591880:CDS 30.0%
AAAAGAAACACTTGCAAAGA+AGG + chr4_2:16845306-16845325 Msa0591880:CDS 30.0%
AATTGCATCAACGATTTCTA+TGG + chr4_2:16845350-16845369 Msa0591880:CDS 30.0%
ATTGCATCAACGATTTCTAT+GGG + chr4_2:16845351-16845370 Msa0591880:CDS 30.0%
! AATGTCGTTTGCAGAAATAT+AGG + chr4_2:16845671-16845690 Msa0591880:intron 30.0%
TAGGATTCATTTGAAGAGAT+CGG + chr4_2:16845690-16845709 Msa0591880:CDS 30.0%
!! CTGAATGGATTGATCAAAAT+CGG + chr4_2:16845767-16845786 Msa0591880:CDS 30.0%
GAAAGTGATGTCATGATCAA+TGG - chr4_2:16844644-16844663 None:intergenic 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4_2:16844841-16844860 None:intergenic 35.0%
TAATGCTCATATGCCAAACA+TGG + chr4_2:16844908-16844927 Msa0591880:CDS 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4_2:16844950-16844969 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG + chr4_2:16845176-16845195 Msa0591880:CDS 35.0%
TCTTATAACATGTTGCCACA+TGG - chr4_2:16845194-16845213 None:intergenic 35.0%
AGAAATCGTTGATGCAATTC+TGG - chr4_2:16845349-16845368 None:intergenic 35.0%
! AGGAAAGAAGCATACTATCA+AGG - chr4_2:16845393-16845412 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+TGG + chr4_2:16845507-16845526 Msa0591880:CDS 35.0%
AATTATGGATGTTCCTGGTT+TGG + chr4_2:16845512-16845531 Msa0591880:CDS 35.0%
ATTATGGATGTTCCTGGTTT+GGG + chr4_2:16845513-16845532 Msa0591880:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4_2:16845542-16845561 Msa0591880:CDS 35.0%
!! TTTACAGGTTTGTTTTCCGA+AGG + chr4_2:16845557-16845576 Msa0591880:intron 35.0%
ACGGGAAGAAAAAAACTAAG+TGG + chr4_2:16845728-16845747 Msa0591880:CDS 35.0%
GAAAGTGATGTCATGATCAA+TGG - chr4_2:16844644-16844663 None:intergenic 35.0%
ATCAGAAGCTGTGTTACATT+TGG - chr4_2:16844841-16844860 None:intergenic 35.0%
TAATGCTCATATGCCAAACA+TGG + chr4_2:16844908-16844927 Msa0591880:CDS 35.0%
! TGAGTTGTAGGGTAACATTT+TGG - chr4_2:16844950-16844969 None:intergenic 35.0%
AAAAACAGTTCAAGACCATG+TGG + chr4_2:16845176-16845195 Msa0591880:CDS 35.0%
TCTTATAACATGTTGCCACA+TGG - chr4_2:16845194-16845213 None:intergenic 35.0%
AGAAATCGTTGATGCAATTC+TGG - chr4_2:16845349-16845368 None:intergenic 35.0%
! AGGAAAGAAGCATACTATCA+AGG - chr4_2:16845393-16845412 None:intergenic 35.0%
CTCAAAATTATGGATGTTCC+TGG + chr4_2:16845507-16845526 Msa0591880:CDS 35.0%
AATTATGGATGTTCCTGGTT+TGG + chr4_2:16845512-16845531 Msa0591880:CDS 35.0%
ATTATGGATGTTCCTGGTTT+GGG + chr4_2:16845513-16845532 Msa0591880:CDS 35.0%
ACATGTTGCTAGTCATTTAC+AGG + chr4_2:16845542-16845561 Msa0591880:CDS 35.0%
!! TTTACAGGTTTGTTTTCCGA+AGG + chr4_2:16845557-16845576 Msa0591880:intron 35.0%
ACGGGAAGAAAAAAACTAAG+TGG + chr4_2:16845728-16845747 Msa0591880:CDS 35.0%
CACTTTCCTCAACTCTATTG+AGG + chr4_2:16844657-16844676 Msa0591880:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_2:16844666-16844685 None:intergenic 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4_2:16844961-16844980 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4_2:16844962-16844981 None:intergenic 40.0%
TCGGCTGATGAAACAAAACA+CGG + chr4_2:16845709-16845728 Msa0591880:CDS 40.0%
!! ACGATGACTTTCTAGCTGAA+TGG + chr4_2:16845791-16845810 Msa0591880:CDS 40.0%
!! TAGCTGAATGGATTGACTGT+GGG + chr4_2:16845803-16845822 Msa0591880:CDS 40.0%
ATCCTACCTTCACAACCTTA+TGG + chr4_2:16845831-16845850 Msa0591880:CDS 40.0%
CACTTTCCTCAACTCTATTG+AGG + chr4_2:16844657-16844676 Msa0591880:CDS 40.0%
ACATCTCCTCAATAGAGTTG+AGG - chr4_2:16844666-16844685 None:intergenic 40.0%
! GACAGGAATGTTGAGTTGTA+GGG - chr4_2:16844961-16844980 None:intergenic 40.0%
! TGACAGGAATGTTGAGTTGT+AGG - chr4_2:16844962-16844981 None:intergenic 40.0%
TCGGCTGATGAAACAAAACA+CGG + chr4_2:16845709-16845728 Msa0591880:CDS 40.0%
!! ACGATGACTTTCTAGCTGAA+TGG + chr4_2:16845791-16845810 Msa0591880:CDS 40.0%
!! TAGCTGAATGGATTGACTGT+GGG + chr4_2:16845803-16845822 Msa0591880:CDS 40.0%
ATCCTACCTTCACAACCTTA+TGG + chr4_2:16845831-16845850 Msa0591880:CDS 40.0%
CAATAACCTTGAGACCAGCA+GGG - chr4_2:16844621-16844640 None:intergenic 45.0%
AATGTGGGGCTTGCGAATAT+TGG + chr4_2:16845137-16845156 Msa0591880:CDS 45.0%
ATTCTGGCGACCAAGACAAA+CGG - chr4_2:16845333-16845352 None:intergenic 45.0%
CTATGGGCAGTCAATCAACT+AGG + chr4_2:16845367-16845386 Msa0591880:CDS 45.0%
CGGCTGATGAAACAAAACAC+GGG + chr4_2:16845710-16845729 Msa0591880:CDS 45.0%
GTGATAGCTCTCAAGCTGAA+TGG + chr4_2:16845752-16845771 Msa0591880:CDS 45.0%
!! CTAGCTGAATGGATTGACTG+TGG + chr4_2:16845802-16845821 Msa0591880:CDS 45.0%
!! AGCTGAATGGATTGACTGTG+GGG + chr4_2:16845804-16845823 Msa0591880:CDS 45.0%
TCTAGGCCATAAGGTTGTGA+AGG - chr4_2:16845840-16845859 None:intergenic 45.0%
TAAACGGCATCTAGGCCATA+AGG - chr4_2:16845849-16845868 None:intergenic 45.0%
CAATAACCTTGAGACCAGCA+GGG - chr4_2:16844621-16844640 None:intergenic 45.0%
AATGTGGGGCTTGCGAATAT+TGG + chr4_2:16845137-16845156 Msa0591880:CDS 45.0%
ATTCTGGCGACCAAGACAAA+CGG - chr4_2:16845333-16845352 None:intergenic 45.0%
CTATGGGCAGTCAATCAACT+AGG + chr4_2:16845367-16845386 Msa0591880:CDS 45.0%
CGGCTGATGAAACAAAACAC+GGG + chr4_2:16845710-16845729 Msa0591880:CDS 45.0%
GTGATAGCTCTCAAGCTGAA+TGG + chr4_2:16845752-16845771 Msa0591880:CDS 45.0%
!! CTAGCTGAATGGATTGACTG+TGG + chr4_2:16845802-16845821 Msa0591880:CDS 45.0%
!! AGCTGAATGGATTGACTGTG+GGG + chr4_2:16845804-16845823 Msa0591880:CDS 45.0%
TCTAGGCCATAAGGTTGTGA+AGG - chr4_2:16845840-16845859 None:intergenic 45.0%
TAAACGGCATCTAGGCCATA+AGG - chr4_2:16845849-16845868 None:intergenic 45.0%
TGAGACCAGCAGGGAATGAT+GGG - chr4_2:16844612-16844631 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG - chr4_2:16844613-16844632 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG + chr4_2:16844612-16844631 Msa0591880:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG - chr4_2:16844622-16844641 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_2:16845320-16845339 Msa0591880:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_2:16845332-16845351 None:intergenic 50.0%
!! GATGTTCCTGGTTTGGGTGT+AGG + chr4_2:16845519-16845538 Msa0591880:CDS 50.0%
ACATGTCCTACACCCAAACC+AGG - chr4_2:16845528-16845547 None:intergenic 50.0%
!! TGAATGGATTGACTGTGGGG+CGG + chr4_2:16845807-16845826 Msa0591880:CDS 50.0%
GGCCATAAGGTTGTGAAGGT+AGG - chr4_2:16845836-16845855 None:intergenic 50.0%
TGAGACCAGCAGGGAATGAT+GGG - chr4_2:16844612-16844631 None:intergenic 50.0%
TTGAGACCAGCAGGGAATGA+TGG - chr4_2:16844613-16844632 None:intergenic 50.0%
! ATCATTCCCTGCTGGTCTCA+AGG + chr4_2:16844612-16844631 Msa0591880:CDS 50.0%
GCAATAACCTTGAGACCAGC+AGG - chr4_2:16844622-16844641 None:intergenic 50.0%
CAAAGAAGGCCCGTTTGTCT+TGG + chr4_2:16845320-16845339 Msa0591880:CDS 50.0%
TTCTGGCGACCAAGACAAAC+GGG - chr4_2:16845332-16845351 None:intergenic 50.0%
!! GATGTTCCTGGTTTGGGTGT+AGG + chr4_2:16845519-16845538 Msa0591880:CDS 50.0%
ACATGTCCTACACCCAAACC+AGG - chr4_2:16845528-16845547 None:intergenic 50.0%
!! TGAATGGATTGACTGTGGGG+CGG + chr4_2:16845807-16845826 Msa0591880:CDS 50.0%
GGCCATAAGGTTGTGAAGGT+AGG - chr4_2:16845836-16845855 None:intergenic 50.0%
GACAACCCATCATTCCCTGC+TGG + chr4_2:16844604-16844623 Msa0591880:CDS 55.0%
!! GAATGGATTGACTGTGGGGC+GGG + chr4_2:16845808-16845827 Msa0591880:CDS 55.0%
GACAACCCATCATTCCCTGC+TGG + chr4_2:16844604-16844623 Msa0591880:CDS 55.0%
!! GAATGGATTGACTGTGGGGC+GGG + chr4_2:16845808-16845827 Msa0591880:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 16844589 16845875 16844589 ID=Msa0591880;Name=Msa0591880
chr4_2 mRNA 16844589 16845875 16844589 ID=Msa0591880-mRNA-1;Parent=Msa0591880;Name=Msa0591880-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.25|0.2|1|1|1|5|17|274
chr4_2 exon 16844589 16844706 16844589 ID=Msa0591880-mRNA-1:exon:4853;Parent=Msa0591880-mRNA-1
chr4_2 exon 16844835 16844984 16844835 ID=Msa0591880-mRNA-1:exon:4854;Parent=Msa0591880-mRNA-1
chr4_2 exon 16845092 16845397 16845092 ID=Msa0591880-mRNA-1:exon:4855;Parent=Msa0591880-mRNA-1
chr4_2 exon 16845487 16845563 16845487 ID=Msa0591880-mRNA-1:exon:4856;Parent=Msa0591880-mRNA-1
chr4_2 exon 16845685 16845875 16845685 ID=Msa0591880-mRNA-1:exon:4857;Parent=Msa0591880-mRNA-1
chr4_2 CDS 16844589 16844706 16844589 ID=Msa0591880-mRNA-1:cds;Parent=Msa0591880-mRNA-1
chr4_2 CDS 16844835 16844984 16844835 ID=Msa0591880-mRNA-1:cds;Parent=Msa0591880-mRNA-1
chr4_2 CDS 16845092 16845397 16845092 ID=Msa0591880-mRNA-1:cds;Parent=Msa0591880-mRNA-1
chr4_2 CDS 16845487 16845563 16845487 ID=Msa0591880-mRNA-1:cds;Parent=Msa0591880-mRNA-1
chr4_2 CDS 16845685 16845858 16845685 ID=Msa0591880-mRNA-1:cds;Parent=Msa0591880-mRNA-1
chr4_2 three_prime_UTR 16845859 16845875 16845859 ID=Msa0591880-mRNA-1:three_prime_utr;Parent=Msa0591880-mRNA-1
Gene Sequence

>Msa0591880

ATGGCTGTTTTCGTTGACAACCCATCATTCCCTGCTGGTCTCAAGGTTATTGCCATTGATCATGACATCACTTTCCTCAACTCTATTGAGGAGATGTGTAATCGATTCCATTATCAAGTTACCAAATGTAACACAGCTTCTGATGCCTTTAATTTGTTGGAAAGAATGGACTGTTTCGATGTAATGTTGATTAATGCTCATATGCCAAACATGGATGTTTATGACTTTGTCCAAAATGTTACCCTACAACTCAACATTCCTGTCATTATGATGGCTGTTGATTCCACAGAAAGTTCTATTATGAAGTCTATTGAATGTGGGGCTTGCGAATATTGGACTAAGCCTTTTGTTGAAAAACAGTTCAAGACCATGTGGCAACATGTTATAAGAAAAGGTTTGACTGAAAATAAAGAATATGAAATTGTTGGAAGCTTGGTTGATCAAGAAAATAGAAAACGGGGTAGAGAAGATTCTAATGTCTCAAAAGAAACACTTGCAAAGAAGGCCCGTTTGTCTTGGTCGCCAGAATTGCATCAACGATTTCTATGGGCAGTCAATCAACTAGGCCTTGATAAAGCAAGACCCAAAATGATTCTCAAAATTATGGATGTTCCTGGTTTGGGTGTAGGACATGTTGCTAGTCATTTACAGAAATATAGGATTCATTTGAAGAGATCGGCTGATGAAACAAAACACGGGAAGAAAAAAACTAAGTGGAGTGATAGCTCTCAAGCTGAATGGATTGATCAAAATCGGAACGATGACTTTCTAGCTGAATGGATTGACTGTGGGGCGGGATCCTACCTTCACAACCTTATGGCCTAG
Protein sequence

>Msa0591880

MAVFVDNPSFPAGLKVIAIDHDITFLNSIEEMCNRFHYQVTKCNTASDAFNLLERMDCFDVMLINAHMPNMDVYDFVQNVTLQLNIPVIMMAVDSTESSIMKSIECGACEYWTKPFVEKQFKTMWQHVIRKGLTENKEYEIVGSLVDQENRKRGREDSNVSKETLAKKARLSWSPELHQRFLWAVNQLGLDKARPKMILKIMDVPGLGVGHVASHLQKYRIHLKRSADETKHGKKKTKWSDSSQAEWIDQNRNDDFLAEWIDCGAGSYLHNLMA*